Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000648

Physical Location & Seq

Genomic Coordinates Forward (+)

16306717 .. 16312273

Gene Structure 5557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1187200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGAGCAGAAGCAAAGCATACAAGGATTCAAGGGCCACTCAAGGTTGCCAAATTTTGCAATTCCGAAACGCTACGATCTTCATCTCAAGCTTGATCTCTCGTCAGCATGCGCCTTCTCCGGCGTTGTGGAGATTCACGTTAGCATAGTCGAGGAGACCAAGTTTCTTATCTTGAACGCTCTTGAACTTGATGTTCATGAAGTCTTGTTCACCAGCTCTCATGGCCAACTTTACCGCCCGTGTGATGTTGTTTTGGACGGTGATGATGAATCTCTTGTTTTGGTGTTTGATCAAGCACTTGGTGTTGGTGAAGGAGTTTTGGGGATTGAGTTTTCTGCAGTCCTTAACGCGCACTTGAAAGGGTTCTATAAATGCACTTATATGGATGGAGGAGAGAAGAAGAATATGGCAGTTACTCAATTCGAACCTGTGGATGCACGGCGGTGCTTTCCATGTTGGGACGAACCTGCCCTCAAGGCCACCTTCAAGATAGCAGTAGATGTACCGCCGGAGCTGACAGCTTTGTCCAATATGCCAATTATCAATGAGAAGCTTGATGCAAATGTTAAGACTGTTTATTTTGAGGAGTCTCCTATCATGTCAACTTATTTAGTGGCTGTTGTTGTTGGCGTGTTCGATTATATTGAAGACACAACATCCGACGGGGTACTGGTTCGTGCATATTGCCCTGTTGGGAAGAGTGACAAAGGGGAGTTTGCTCTTAATGTCGCTGTTAAGACGCTCGATCTTTTCTCCAAATACTTTTCAACGCCGTACCCTCTCCCCAAACTCGATATGGTTGCAGTTCCTGAATTTTCTGGTGGGGCAATGGAGAATTATGGTTTGATCACATACCGTGAAAATGAACTGCTTTACGATCCTTTGCATTCCACAACAGCAAGGAAGCAGACAATGGCAATTGTTGTAGCGCACGAAGTAGCACATCAGTGGTTTGGCAACCTGGTGACGATGGAATGGTGGTCCGATTTGTGGCTTAATGAGGGTTTTGCAACATGGGTAAGTTATATGGCCACAGATATTTTATTTCCCGAGTGGAAAATATGGGCTCAATTTCTTCAACAAACTACTGGCGGCCTAGTTAAGGATGCACTGGAGCAGTCACATCCAATTCAGGTGGAAATAGACCATGCTCGCTCAATTCTTGAAGTCTTTGATGATATCAGCTATAAAAAGGGATCAGCTGTTATTCGAATGCTGCAGGGTTATCTTGGGGATGACATATTGCAGAAATCGTTAAGTTCCTACATTAAAAGATTTTCTGGCAAAAATGCGAAGACAGAAGATTTATGGAGTGTTCTTTCGGAGGAATCTGGTGTCAAAGTCAGTGAAATGATGGATGGTTGGACAAAGGAAAAAGGATATCCTGTTATCTCCGTAAAAGCCAAAGAGCATATTTTGGAATTTGAGCAGACACAGTTTTTGTCATCCGGTTCGCATGGTGATGGGAAATGGATTGTTCCAATAACTTTTTCACTTGGTTCGTATGAGAGGCGCAGGAATTTCCTTTTGGAAACAAAGTCCAGAGAAGTGAACATATCAGATCTTGTTAATTCATCTGATAATGATTTAAAGGATAAAGAGAAATATGATGAACAACTGTGGGTCAAGGTTAACATCGAGCAGAGCGGTTTCTATAGGGTCAATTACGAAGATAAGCTTGCAGCTCGGCTTAGGAAGGCTATTGAGCATAATAGCTTAGAAGCAACTGACAAATTCGGCATTCTGGATGATGCATATGCTCTTTGTGAAACTTGTGAACAATCACTTTCATCTTTGCTTTCCTTGATGGATGCATACAGAAAAGAAGAAGAGTATATTGTCGTAACAAAATTGATAGATGTTTGTTATAATGTCATTAATATTTCAAGTGAGGCCATCCCAGATTTGGCAAATGAGTTGAAAAAGTTCTTCATTAGTCTCCTCCTGTTTCCAGCAGAAAAACTTGGTTGGGAATCAGTACCAGGAGAGAATCACTTCGGTGCACTTCTGAGAGCAGAAATCTTGCAAGCTTTGGTTACATTTGGCCATGATAAAACTCAAAAAGATGCATTAGACCGATTTCAGACATTGTTGAACGATAGAAACACTCCGCTTCTTTCAGCTGATACAAGAGGGGCTGCTTACATTGCCGTGATGAGAAATGCTAGCAGCTCCAATAGAGAAGGTTTTGAGTCCCTATTAAATTTTTACAGAGAAGCGAATACAGTGCAAGAGAAGGAAAGGATTCTACGGTTTTTGGCGTCTTCTCCAGACCCAGATACAATTCTGGAGGTTCTGAACTTTTTCTTATCCGAAGAGGTTCGAGATCAAGACATTATATATGGACTTTTTGGTATAAGCTCAGAATGCCGCGAAACAGCGTGGAGGTGGTTAAAGGAGAATTGGGACCTGATCCTGACCAAATACGGCGCTGGGATGCTGCTCACCCATTTCGTAAAAGACATTGTGACACCGCTTTGCAGCAACGAAAAAGCTGATGAGGTTGAAGAATTTTTCGCTAGCCGGGCGCATCCTGCAATTTCCATGACTTTGGAGCAAAGCATTGCGCAAGTCCGAATCAAGGCGAGGTGGGTAGAACACATGAGGCAAGAGCAATCCCTTGAAGGGCAAGTCAGAGAACTAGCAGGCAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006518 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009628 GO:0009987 GO:0016787 GO:0019538 GO:0033218 GO:0033554 GO:0034641 GO:0036293 GO:0036294 GO:0042221 GO:0042277 GO:0043167 GO:0043169 GO:0043170 GO:0043171 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0070006 GO:0070011 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ERAP1_C Peptidase_M1

Protein Analysis

886

Amino Acids

100.32

Weight (kDa)

4.99

Isoelectric Point (pI)

40.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	22 - 204	1.6e-49	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	239 - 455	8.4e-85	Peptidase family M1 domain
ERAP1_C	PF11838	541 - 859	2.8e-77	ERAP1-like C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000648)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33090
fragaria_vesca	FvH4_2g31431 FvH4_2g31440 FvH4_6g34250 FvH4_6g34262 FvH4_6g34263
malus_domestica	MD08G1066400.v1.1 MD08G1066500.v1.1 MD08G1067400.v1.1 MD09G1070300.v1.1 MD09G1187200.v1.1 MD10G1090900.v1.1 MD15G1054400.v1.1 MD17G1171400.v1.1
prunus_persica	Prupe.1G407900_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G457300_v2.0.a1 Prupe.3G044500_v2.0.a1 Prupe.3G044500_v2.0.a1
pyrus_communis	pycom08g05520 pycom08g05550 pycom08g05560 pycom08g05590 pycom09g10370 pycom09g10380 pycom10g07440 pycom10g07460 pycom15g05130 pycom15g05150 pycom17g16900
rosa_chinensis	RchiOBHm_Chr2g0144571 RchiOBHm_Chr2g0144611 RchiOBHm_Chr4g0390591 RchiOBHm_Chr6g0311371 RchiOBHm_Chr6g0311381
rosa_laevigata	RLG00000009010 RLG00000009289 RLG00000010411 RLG00000010412 RLG00000020090 RLG00000020092 RLG00000036974
rosa_multiflora	Rmu_co8212940.1_g000001 Rmu_sc0005789.1_g000001 Rmu_sc0008536.1_g000010 Rmu_sc0008536.1_g000011 Rmu_sc0009300.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056400 Rroxscaffold_2G00084720 Rroxscaffold_2G00101200 Rroxscaffold_7G00157670 Rroxscaffold_7G00157680
rosa_rugosa	Rorug02G0387900 Rorug02G0388000 Rorug02G0388100 Rorug06G0388300 Rorug06G0388400
rosa_samantha	Rh2AG440000 Rh2AG440100 Rh2BG450000 Rh2BG450300 Rh2BG450500 Rh2CG427000 Rh2CG427200 Rh2DG459900 Rh2DG460300 Rh2DG460500 Rh6AG502600 Rh6AG502700 Rh6BG513300 Rh6CG519100 Rh6CG519200
rosa_wichuraiana	Rw2G036000 Rw6G043770 Rw6G043780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1869
AasI	GACNNNNNNGTC	1 cut(s)	523
Acc16I	TGCGCA	1 cut(s)	2568
Acc36I	ACCTGC	1 cut(s)	475
AccBSI	CCGCTC	1 cut(s)	1647
AccII	CGCG	2 cut(s)	350, 2373
AciI	CCGC	8 cut(s)	235, 442, 506, 1092, 1647, 2111, 2371, 2474
AclWI	GGATC	3 cut(s)	872, 1204, 2407
AcoI	YGGCCR	3 cut(s)	223, 1029, 2044
AcsI	RAATTY	7 cut(s)	52, 812, 1421, 1519, 1733, 2203, 2510
AcyI	GRCGYC	1 cut(s)	2261
AdeI	CACNNNGTG	1 cut(s)	302
AfaI	GTAC	4 cut(s)	504, 669, 776, 1980
AfiI	CCNNNNNNNGG	5 cut(s)	990, 1102, 1906, 2581, 2625
AjnI	CCWGG	2 cut(s)	960, 1981
AjuI	GAANNNNNNNTTGG	4 cut(s)	1474, 1506, 1511, 1543
AleI	CACNNNNGTG	3 cut(s)	442, 946, 1998
Alw21I	GWGCWC	1 cut(s)	2005
Alw26I	GTCTC	3 cut(s)	149, 594, 1943
Alw44I	GTGCAC	1 cut(s)	2001
AlwI	GGATC	3 cut(s)	872, 1204, 2407
AlwNI	CAGNNNCTG	1 cut(s)	809
Ama87I	CYCGRG	1 cut(s)	1049
AoxI	GGCC	7 cut(s)	34, 223, 477, 1029, 1093, 1893, 2044
ApaLI	GTGCAC	1 cut(s)	2001
ApeKI	GCWGC	6 cut(s)	1216, 1682, 2138, 2169, 2440, 2481
ApoI	RAATTY	7 cut(s)	52, 812, 1421, 1519, 1733, 2203, 2510
ArsI	GACNNNNNNTTYG	2 cut(s)	1423, 1455
AseI	ATTAAT	1 cut(s)	1878
Asp700I	GAANNNNTTC	6 cut(s)	362, 1925, 1994, 2244, 2303, 2319
AspLEI	GCGC	7 cut(s)	113, 352, 931, 1515, 2432, 2530, 2569
AspS9I	GGNCC	3 cut(s)	34, 981, 2407
AsuC2I	CCSGG	1 cut(s)	2525
AsuHPI	GGTGA	6 cut(s)	202, 272, 320, 976, 1472, 2437
AsuII	TTCGAA	2 cut(s)	423, 1210
AsuNHI	GCTAGC	2 cut(s)	2165, 2519
AvaI	CYCGRG	1 cut(s)	1049
AvaII	GGWCC	2 cut(s)	981, 2407
BaeGI	GKGCMC	1 cut(s)	2005
BalI	TGGCCA	3 cut(s)	225, 1031, 2046
BanII	GRGCYC	1 cut(s)	1069
BbsI	GAAGAC	3 cut(s)	654, 1301, 2256
Bbv12I	GWGCWC	1 cut(s)	2005
BbvI	GCAGC	6 cut(s)	1203, 1694, 2125, 2181, 2427, 2493
BccI	CCATC	7 cut(s)	380, 964, 1348, 1352, 1457, 1801, 1904
BceAI	ACGGC	4 cut(s)	455, 757, 2135, 2443
BcgI	CGANNNNNNTGC	4 cut(s)	782, 816, 2209, 2243
BciT130I	CCWGG	2 cut(s)	962, 1983
BclI	TGATCA	2 cut(s)	289, 846
BcnI	CCSGG	1 cut(s)	2525
BcoDI	GTCTC	3 cut(s)	149, 594, 1943
BfaI	CTAG	4 cut(s)	1097, 2166, 2520, 2642
BfmI	CTRYAG	3 cut(s)	336, 1217, 1654
BfoI	RGCGCY	1 cut(s)	2433
BfuAI	ACCTGC	1 cut(s)	475
BglI	GCCNNNNNGGC	1 cut(s)	476
BglII	AGATCT	1 cut(s)	1561
BisI	GCNGC	8 cut(s)	1093, 1217, 1683, 2139, 2170, 2371, 2441, 2482
BlsI	GCNGC	8 cut(s)	1094, 1218, 1684, 2140, 2171, 2372, 2442, 2483
Bme1390I	CCNGG	3 cut(s)	962, 1983, 2525
Bme18I	GGWCC	2 cut(s)	981, 2407
BmeT110I	CYCGRG	1 cut(s)	1049
BmgT120I	GGNCC	3 cut(s)	34, 981, 2407
BmiI	GGNNCC	1 cut(s)	2408
BmrFI	CCNGG	3 cut(s)	962, 1983, 2525
BmsI	GCATC	8 cut(s)	424, 547, 1096, 1741, 1801, 2056, 2427, 2539
BmtI	GCTAGC	2 cut(s)	2169, 2523
BpiI	GAAGAC	3 cut(s)	654, 1301, 2256
BpmI	CTGGAG	3 cut(s)	1133, 2253, 2309
Bpu10I	CCTNAGC	1 cut(s)	1691
Bpu14I	TTCGAA	2 cut(s)	423, 1210
BpuEI	CTTGAG	3 cut(s)	25, 71, 458
BpuMI	CCSGG	1 cut(s)	2525
BsaBI	GATNNNNATC	1 cut(s)	81
BsaHI	GRCGYC	1 cut(s)	2261
BsaI	GGTCTC	1 cut(s)	149
BsaWI	WCCGGW	1 cut(s)	1448
BsaXI	ACNNNNNCTCC	2 cut(s)	146, 176
Bsc4I	CCNNNNNNNGG	5 cut(s)	990, 1102, 1906, 2581, 2625
Bse1I	ACTGG	3 cut(s)	675, 1093, 1116
Bse3DI	GCAATG	3 cut(s)	834, 2145, 2562
Bse8I	GATNNNNATC	1 cut(s)	81
BseBI	CCWGG	2 cut(s)	962, 1983
BseJI	GATNNNNATC	1 cut(s)	81
BseLI	CCNNNNNNNGG	5 cut(s)	990, 1102, 1906, 2581, 2625
BseMI	GCAATG	3 cut(s)	834, 2145, 2562
BseMII	CTCAG	2 cut(s)	2000, 2376
BseNI	ACTGG	3 cut(s)	675, 1093, 1116
BseRI	GAGGAG	4 cut(s)	167, 405, 599, 1931
BseSI	GKGCMC	1 cut(s)	2005
BseXI	GCAGC	6 cut(s)	1203, 1694, 2125, 2181, 2427, 2493
BseYI	CCCAGC	1 cut(s)	2432
Bsh1236I	CGCG	2 cut(s)	350, 2373
BshFI	GGCC	7 cut(s)	36, 225, 479, 1031, 1095, 1895, 2046
BsiHKAI	GWGCWC	1 cut(s)	2005
BsiHKCI	CYCGRG	1 cut(s)	1049
BsiSI	CCGG	4 cut(s)	120, 509, 1449, 2524
BslFI	GGGAC	3 cut(s)	473, 2179, 2420
BslI	CCNNNNNNNGG	5 cut(s)	990, 1102, 1906, 2581, 2625
BsmAI	GTCTC	3 cut(s)	149, 594, 1943
BsmFI	GGGAC	3 cut(s)	473, 2179, 2420
BsmI	GAATGC	4 cut(s)	886, 1218, 1740, 2372
BsnI	GGCC	7 cut(s)	36, 225, 479, 1031, 1095, 1895, 2046
Bso31I	GGTCTC	1 cut(s)	149
BsoBI	CYCGRG	1 cut(s)	1049
Bsp119I	TTCGAA	2 cut(s)	423, 1210
Bsp1286I	GDGCHC	2 cut(s)	1069, 2005
BspACI	CCGC	8 cut(s)	235, 442, 506, 1092, 1647, 2111, 2371, 2474
BspANI	GGCC	7 cut(s)	36, 225, 479, 1031, 1095, 1895, 2046
BspCNI	CTCAG	2 cut(s)	2001, 2375
BspFNI	CGCG	2 cut(s)	350, 2373
BspHI	TCATGA	1 cut(s)	196
BspLI	GGNNCC	1 cut(s)	2408
BspMAI	CTGCAG	2 cut(s)	340, 1221
BspMI	ACCTGC	1 cut(s)	475
BspOI	GCTAGC	2 cut(s)	2169, 2523
BspPI	GGATC	3 cut(s)	872, 1204, 2407
BspT104I	TTCGAA	2 cut(s)	423, 1210
BspTNI	GGTCTC	1 cut(s)	149
BsrBI	CCGCTC	1 cut(s)	1647
BsrDI	GCAATG	3 cut(s)	834, 2145, 2562
BsrI	ACTGG	3 cut(s)	675, 1093, 1116
BssNI	GRCGYC	1 cut(s)	2261
Bst2UI	CCWGG	2 cut(s)	962, 1983
Bst4CI	ACNGT	7 cut(s)	260, 574, 857, 1437, 1620, 2227, 2253
Bst6I	CTCTTC	3 cut(s)	692, 1824, 2310
BstACI	GRCGYC	1 cut(s)	2261
BstBI	TTCGAA	2 cut(s)	423, 1210
BstC8I	GCNNGC	7 cut(s)	109, 1153, 1680, 2028, 2167, 2521, 2647
BstDEI	CTNAG	4 cut(s)	1691, 1717, 2009, 2362
BstENI	CCTNNNNNAGG	1 cut(s)	1100
BstFNI	CGCG	2 cut(s)	350, 2373
BstH2I	RGCGCY	1 cut(s)	2433
BstHHI	GCGC	7 cut(s)	113, 352, 931, 1515, 2432, 2530, 2569
BstMAI	GTCTC	3 cut(s)	149, 594, 1943
BstMWI	GCNNNNNNNGC	6 cut(s)	222, 476, 2147, 2367, 2525, 2534
BstNI	CCWGG	2 cut(s)	962, 1983
BstNSI	RCATGY	1 cut(s)	111
BstSCI	CCNGG	3 cut(s)	960, 1981, 2523
BstSFI	CTRYAG	3 cut(s)	336, 1217, 1654
BstSLI	GKGCMC	1 cut(s)	2005
BstUI	CGCG	2 cut(s)	350, 2373
BstV1I	GCAGC	6 cut(s)	1203, 1694, 2125, 2181, 2427, 2493
BstV2I	GAAGAC	3 cut(s)	654, 1301, 2256
BstX2I	RGATCY	1 cut(s)	1561
BstXI	CCANNNNNNTGG	1 cut(s)	2551
BstYI	RGATCY	1 cut(s)	1561
BsuRI	GGCC	7 cut(s)	36, 225, 479, 1031, 1095, 1895, 2046
BtsIMutI	CAGTG	4 cut(s)	953, 1109, 1351, 2232
BveI	ACCTGC	1 cut(s)	475
Cac8I	GCNNGC	7 cut(s)	109, 1153, 1680, 2028, 2167, 2521, 2647
CaiI	CAGNNNCTG	1 cut(s)	809
CciI	TCATGA	1 cut(s)	196
CfoI	GCGC	7 cut(s)	113, 352, 931, 1515, 2432, 2530, 2569
Cfr13I	GGNCC	3 cut(s)	34, 981, 2407
CseI	GACGC	2 cut(s)	748, 2250
CsiI	ACCWGGT	1 cut(s)	960
Csp6I	GTAC	4 cut(s)	503, 668, 775, 1979
CspCI	CAANNNNNGTGG	2 cut(s)	1116, 1151
CviQI	GTAC	4 cut(s)	503, 668, 775, 1979
DdeI	CTNAG	4 cut(s)	1691, 1717, 2009, 2362
DraI	TTTAAA	1 cut(s)	1590
DraIII	CACNNNGTG	1 cut(s)	302
DrdI	GACNNNNNNGTC	1 cut(s)	523
DseDI	GACNNNNNNGTC	1 cut(s)	523
EaeI	YGGCCR	3 cut(s)	223, 1029, 2044
Eam1104I	CTCTTC	3 cut(s)	692, 1824, 2310
EarI	CTCTTC	3 cut(s)	692, 1824, 2310
Eco24I	GRGCYC	1 cut(s)	1069
Eco31I	GGTCTC	1 cut(s)	149
Eco32I	GATATC	2 cut(s)	1180, 1382
Eco47I	GGWCC	2 cut(s)	981, 2407
Eco88I	CYCGRG	1 cut(s)	1049
EcoNI	CCTNNNNNAGG	1 cut(s)	1100
EcoO109I	RGGNCCY	1 cut(s)	2407
EcoRII	CCWGG	2 cut(s)	960, 1981
EcoRV	GATATC	2 cut(s)	1180, 1382
EcoT22I	ATGCAT	3 cut(s)	1756, 1816, 2071
EcoT38I	GRGCYC	1 cut(s)	1069
FalI	AAGNNNNNCTT	4 cut(s)	467, 499, 1662, 1694
FaqI	GGGAC	3 cut(s)	473, 2179, 2420
FauNDI	CATATG	1 cut(s)	1756
FbaI	TGATCA	2 cut(s)	289, 846
Fnu4HI	GCNGC	8 cut(s)	1093, 1217, 1683, 2139, 2170, 2371, 2441, 2482
FriOI	GRGCYC	1 cut(s)	1069
Fsp4HI	GCNGC	8 cut(s)	1093, 1217, 1683, 2139, 2170, 2371, 2441, 2482
FspBI	CTAG	4 cut(s)	1097, 2166, 2520, 2642
FspI	TGCGCA	1 cut(s)	2568
GlaI	GCGC	7 cut(s)	112, 351, 930, 1514, 2431, 2529, 2568
GluI	GCNGC	8 cut(s)	1093, 1217, 1683, 2139, 2170, 2371, 2441, 2482
GsaI	CCCAGC	1 cut(s)	2436
GsuI	CTGGAG	3 cut(s)	1133, 2253, 2309
HaeII	RGCGCY	1 cut(s)	2433
HaeIII	GGCC	7 cut(s)	36, 225, 479, 1031, 1095, 1895, 2046
HapII	CCGG	4 cut(s)	120, 509, 1449, 2524
HgaI	GACGC	2 cut(s)	748, 2250
HhaI	GCGC	7 cut(s)	113, 352, 931, 1515, 2432, 2530, 2569
Hin1I	GRCGYC	1 cut(s)	2261
Hin6I	GCGC	7 cut(s)	111, 350, 929, 1513, 2430, 2528, 2567
HinP1I	GCGC	7 cut(s)	111, 350, 929, 1513, 2430, 2528, 2567
HincII	GTYRAC	2 cut(s)	603, 1633
HindII	GTYRAC	2 cut(s)	603, 1633
HindIII	AAGCTT	4 cut(s)	89, 551, 1676, 2028
HpaI	GTTAAC	1 cut(s)	1633
HpaII	CCGG	4 cut(s)	120, 509, 1449, 2524
HphI	GGTGA	6 cut(s)	202, 272, 320, 976, 1472, 2437
Hpy166II	GTNNAC	6 cut(s)	210, 603, 1552, 1633, 1778, 2003
Hpy8I	GTNNAC	6 cut(s)	210, 603, 1552, 1633, 1778, 2003
Hpy99I	CGWCG	1 cut(s)	665
HpyAV	CCTTC	7 cut(s)	124, 305, 493, 1690, 2177, 2230, 2618
HpyCH4III	ACNGT	7 cut(s)	260, 574, 857, 1437, 1620, 2227, 2253
HpyCH4IV	ACGT	1 cut(s)	138
HpyF10VI	GCNNNNNNNGC	6 cut(s)	222, 476, 2147, 2367, 2525, 2534
HpyF3I	CTNAG	4 cut(s)	1691, 1717, 2009, 2362
HpySE526I	ACGT	1 cut(s)	138
Hsp92I	GRCGYC	1 cut(s)	2261
HspAI	GCGC	7 cut(s)	111, 350, 929, 1513, 2430, 2528, 2567
Ksp22I	TGATCA	2 cut(s)	289, 846
KspAI	GTTAAC	1 cut(s)	1633
LmnI	GCTCC	5 cut(s)	4, 511, 1114, 2177, 2554
Lsp1109I	GCAGC	6 cut(s)	1203, 1694, 2125, 2181, 2427, 2493
LweI	GCATC	8 cut(s)	424, 547, 1096, 1741, 1801, 2056, 2427, 2539
MabI	ACCWGGT	1 cut(s)	960
MaeI	CTAG	4 cut(s)	1097, 2166, 2520, 2642
MaeII	ACGT	1 cut(s)	138
MaeIII	GTNAC	7 cut(s)	412, 701, 964, 1119, 1843, 2035, 2467
MbiI	CCGCTC	1 cut(s)	1647
MfeI	CAATTG	1 cut(s)	918
MflI	RGATCY	1 cut(s)	1561
MhlI	GDGCHC	2 cut(s)	1069, 2005
MlsI	TGGCCA	3 cut(s)	225, 1031, 2046
MluNI	TGGCCA	3 cut(s)	225, 1031, 2046
MlyI	GAGTC	2 cut(s)	596, 2201
MmeI	TCCRAC	2 cut(s)	684, 1343
Mox20I	TGGCCA	3 cut(s)	225, 1031, 2046
Mph1103I	ATGCAT	3 cut(s)	1756, 1816, 2071
MroXI	GAANNNNTTC	6 cut(s)	362, 1925, 1994, 2244, 2303, 2319
MscI	TGGCCA	3 cut(s)	225, 1031, 2046
MslI	CAYNNNNRTG	6 cut(s)	380, 442, 946, 1461, 1998, 2150
Msp20I	TGGCCA	3 cut(s)	225, 1031, 2046
MspA1I	CMGCKG	2 cut(s)	1202, 2123
MspI	CCGG	4 cut(s)	120, 509, 1449, 2524
MspR9I	CCNGG	3 cut(s)	962, 1983, 2525
MunI	CAATTG	1 cut(s)	918
Mva1269I	GAATGC	4 cut(s)	886, 1218, 1740, 2372
MvaI	CCWGG	2 cut(s)	962, 1983
MvnI	CGCG	2 cut(s)	350, 2373
MwoI	GCNNNNNNNGC	6 cut(s)	222, 476, 2147, 2367, 2525, 2534
NciI	CCSGG	1 cut(s)	2525
NdeI	CATATG	1 cut(s)	1756
NheI	GCTAGC	2 cut(s)	2165, 2519
NlaIV	GGNNCC	1 cut(s)	2408
NmeAIII	GCCGAG	1 cut(s)	1666
NmuCI	GTSAC	4 cut(s)	701, 964, 1119, 2467
NsbI	TGCGCA	1 cut(s)	2568
NsiI	ATGCAT	3 cut(s)	1756, 1816, 2071
NspI	RCATGY	1 cut(s)	111
NspV	TTCGAA	2 cut(s)	423, 1210
OliI	CACNNNNGTG	3 cut(s)	442, 946, 1998
PaeI	GCATGC	1 cut(s)	111
PagI	TCATGA	1 cut(s)	196
PctI	GAATGC	4 cut(s)	886, 1218, 1740, 2372
PdmI	GAANNNNTTC	6 cut(s)	362, 1925, 1994, 2244, 2303, 2319
PfeI	GAWTC	8 cut(s)	27, 133, 269, 1328, 1973, 1990, 2245, 2577
PkrI	GCNGC	8 cut(s)	1094, 1218, 1684, 2140, 2171, 2372, 2442, 2483
PleI	GAGTC	2 cut(s)	595, 2200
PpsI	GAGTC	2 cut(s)	595, 2200
PpuMI	RGGWCCY	1 cut(s)	2407
PshBI	ATTAAT	1 cut(s)	1878
PsiI	TTATAA	1 cut(s)	1869
Psp5II	RGGWCCY	1 cut(s)	2407
Psp6I	CCWGG	2 cut(s)	960, 1981
PspFI	CCCAGC	1 cut(s)	2432
PspGI	CCWGG	2 cut(s)	960, 1981
PspN4I	GGNNCC	1 cut(s)	2408
PspPI	GGNCC	3 cut(s)	34, 981, 2407
PspPPI	RGGWCCY	1 cut(s)	2407
PstI	CTGCAG	2 cut(s)	340, 1221
PstNI	CAGNNNCTG	1 cut(s)	809
PsuI	RGATCY	1 cut(s)	1561
PvuII	CAGCTG	2 cut(s)	1202, 2123
RsaI	GTAC	4 cut(s)	504, 669, 776, 1980
RsaNI	GTAC	4 cut(s)	503, 668, 775, 1979
RseI	CAYNNNNRTG	6 cut(s)	380, 442, 946, 1461, 1998, 2150
SatI	GCNGC	8 cut(s)	1093, 1217, 1683, 2139, 2170, 2371, 2441, 2482
Sau96I	GGNCC	3 cut(s)	34, 981, 2407
SchI	GAGTC	2 cut(s)	596, 2201
ScrFI	CCNGG	3 cut(s)	962, 1983, 2525
SduI	GDGCHC	2 cut(s)	1069, 2005
SexAI	ACCWGGT	1 cut(s)	960
SfaNI	GCATC	8 cut(s)	424, 547, 1096, 1741, 1801, 2056, 2427, 2539
SfcI	CTRYAG	3 cut(s)	336, 1217, 1654
SfuI	TTCGAA	2 cut(s)	423, 1210
SinI	GGWCC	2 cut(s)	981, 2407
SmiMI	CAYNNNNRTG	6 cut(s)	380, 442, 946, 1461, 1998, 2150
SmlI	CTYRAG	3 cut(s)	40, 86, 473
SmoI	CTYRAG	3 cut(s)	40, 86, 473
SphI	GCATGC	1 cut(s)	111
SsiI	CCGC	8 cut(s)	235, 442, 506, 1092, 1647, 2111, 2371, 2474
SspI	AATATT	1 cut(s)	1882
SspMI	CTAG	4 cut(s)	1097, 2166, 2520, 2642
StyD4I	CCNGG	3 cut(s)	960, 1981, 2523
TaaI	ACNGT	7 cut(s)	260, 574, 857, 1437, 1620, 2227, 2253
TaiI	ACGT	1 cut(s)	141
TaqI	TCGA	8 cut(s)	150, 423, 636, 744, 792, 1210, 1638, 2323
TaqII	GACCGA	1 cut(s)	2091
TauI	GCSGC	2 cut(s)	1095, 2373
TfiI	GAWTC	8 cut(s)	27, 133, 269, 1328, 1973, 1990, 2245, 2577
TscAI	CASTG	4 cut(s)	953, 1116, 1351, 2232
TseFI	GTSAC	4 cut(s)	701, 964, 1119, 2467
TseI	GCWGC	6 cut(s)	1216, 1682, 2138, 2169, 2440, 2481
Tsp45I	GTSAC	4 cut(s)	701, 964, 1119, 2467
TspDTI	ATGAA	9 cut(s)	71, 185, 213, 282, 879, 1563, 1626, 1779, 1921
TspGWI	ACGGA	1 cut(s)	1384
TspRI	CASTG	4 cut(s)	953, 1116, 1351, 2232
VneI	GTGCAC	1 cut(s)	2001
VpaK11BI	GGWCC	2 cut(s)	981, 2407
VspI	ATTAAT	1 cut(s)	1878
XagI	CCTNNNNNAGG	1 cut(s)	1100
XapI	RAATTY	7 cut(s)	52, 812, 1421, 1519, 1733, 2203, 2510
XceI	RCATGY	1 cut(s)	111
XcmI	CCANNNNNNNNNTGG	2 cut(s)	793, 1903
XmnI	GAANNNNTTC	6 cut(s)	362, 1925, 1994, 2244, 2303, 2319
XspI	CTAG	4 cut(s)	1097, 2166, 2520, 2642
Zsp2I	ATGCAT	3 cut(s)	1756, 1816, 2071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.