Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000648

Physical Location & Seq

Genomic Coordinates Reverse (-)

13615192 .. 13621226

Gene Structure 6035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1090900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGGAGCAGTTCAAAGGCCAACCGCGGCTTCCTAAATTCGCCGTGCCAAAGCGATACTCCATACGTCTGAAACCCGACCTCATCGCTTGCAAATTCGCCGGCTCCGTCTCCATCGACCTCGATATCGTCTCCGATACTCGATTCATCGCCCTCAACGCTGCCGAGCTCTCCGTCGATGCCGCTTCCGTCTCTTTCACTCACTCTTCCAAGATCTTTAAGCCTTCAAAAGTTGAGATATTCGACGAAGACGGGGTTTTGGTTTTGGAGTTTGGAGAGACACTTCCGATTGGTTTGGGAGTTTTGGCTATTGGGTTTGAAGGTATTTTGAATGACAAGATGAAGGGATTTTTTAGAAGTACATATGAGCATAACAGTGAGAAGAAAAATATGGCAGTTACGCAGTTTCAACCAGTTGATGCCAGGCGGTGCTTTCCATGTTGGGATGAACCTGCTTGGAAGGCTACATTCAAGATTATATTGGATGATGTTCCGTCCGAATTAGTAGCTCTTTCCAACATGCCGGTTGGAGAAGAAAAAGTGGATGGACATATGAAGACAGTTACATATCAAGAATCTCCAATCATGTCTACATATTTGGTGGCCATAGTTGTTGGGTTGTTTGATTATATTGAAGATCATACTTCTGATGGGGTCATAGTGCGGGTATATTGTCCTGTTGGCAAGGCAGACCAAGGGAAGTTTGCTTTGGACCTTGCTGTCAGGACTCTTGAATTTTACAAAGATTATTTTTCTGTGCCGTACTCTTTGCCCAAATTGGATATGGTTGCAATTCCTGATTTTCTTGGGGCCATGGAGAATTATGGTTTAGTTACGTACCAAGAATCAGCTTTGCTCTTTGATGAACAAAATTCTGCAGCTGCCAACAAGCAAATGGTTTCAACTGCTGTGACACATGAATTGGCGCACCAGTGGTTTGGCAATCTTGTAACAATGGAATGGTGGACACACTTATGGCTGAACGAGGGATTTGCAACATGGGTGAGCTATTTAGCTACTGATAGCTTGTTCCCAGAGTGGAAAGTATGGACTCAGTTTGTTGATGAAAGTGCTAGGGGTCTTAAGCTGGATGGGCTTGAAGAATCTCACCCCATCGAGGTAGAGATCAATCATGCTGCTGAGGTTGATGAAATATTTGATGCAATAAGTTATAGAAAAGGAGCTTCTATTATTCGGATGCTACAAAGCTATCTTGGTGCGGAATGCTTCCAGTCACTTGCTTCATACATAAGAAAGCATGCTTCCTCAAATACTTTCACAGAAGATTTATGGGCTGCCCTTGAGGAGATATCTGGTGAACCTGTGAACAAGCTAATGAATTCATGGACTCAGCAAATGGGTTACCCTGTTATTTCTGTCAAAGTGAAAGATCAGAAATTGCAATTTGACCAGACACAATTCTACTCGAGTGGTTCCCAAGGTGATGGGCAATGGATTGTGCCAATAACATTATGCTGTGGCTCATATGATGTGCACAAGAGATTCCTACTACGAAACAAGTCTGAAAGTCTCGACATAAAAGAATTTCTGGGCTGCTCCCTAGCGGAGAAGGGATGTAGAAGTGCAAACAATAAAAACAATGCCTTATGCAGTTGGATCAAAGTCAACTTGGATCAGACTGGTTTTTACAGGGTGATATATTCAGATGAACTTTTACCTGCACTTAGAAATGTTATAGAAAAGAAAGACTTGTCTGCAACTGACAGATACGGAATCTTGGATGATTCATTTGCCCTTTCTATGGCTCGCCAGCAGTCTTTTGCTTCATTGCTTACGTTGTTGGATGCTTACAGAGAGGAACTTGACTATACAGTGCTGTCAAATTTGATTGCCATATGTTATAAGCTGGCAAGAATTGCAGCTGATGCTGTACCCGAGCTACTGAATCTCATAAAACAATCGTTTATTAGCCTTTTCCAGTACTCTGCAGAAAAGCTTGGTTGGGAGCCTAAACCTGGTGAGAGCCATTTAGATTCAATGCTAAGAGGAGAAATTTTAACTGCCCTTGCTGTGTTTGGACATGACCCAACAATTAATGAAGCAAGTAGGCGTTTTCGTGAATTCTTAGATAACCGAAATACGCCAGTCCTTCCTCCCGACATAAGAAAGGCAGTGTATGTGGCGGTAATGCAAAGAGTAAGCATGTCTAACAGATGGGGCTACGAATCTCTTCTTAGAGTTTACAGAGAGGCTGATCTAAGCGAGGAGAAAAACCGCATTATAAAATCATTAGCATCTTGTCCAGATCCGAGCATAGTACTGGAAGTTCTCAACCTTTTATTGTCTTCTGAGGTCCGCCTTCAGGATGCTGCCCGTGGGCTATCTTCTGTTAGTAGTGAAGGACGGGAAACAGCTTTGACGTGGCTGAAGGATAACTGGGAGCACATCTCCAAGACCTGGGGTTCTGGATTTGTAATTGCTAGCTTTGTCAGTGCAGTTGTTTCACCGTTGGCTTCATTTGAAAAGGTTAAGGAAGTAGATGAGTTCTTTAAGGTTCATCCCACCCCCTCGATAACCAGAACCTTGAAGCAGAGCATTGAGCGTGTGCAGATTAACGCCAAGTGGGTTCAGAGTGTTCAGGGTGAGACAGATCTTGCCGATGTTTTGAACGAGTTGGCATACAGGAAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006518 GO:0006807 GO:0006810 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009628 GO:0009914 GO:0009926 GO:0009987 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0031090 GO:0031406 GO:0033218 GO:0033293 GO:0033554 GO:0034641 GO:0036094 GO:0036293 GO:0036294 GO:0042221 GO:0042277 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043177 GO:0043226 GO:0043227 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060918 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ERAP1_C Peptidase_M1

Protein Analysis

884

Amino Acids

99.55

Weight (kDa)

5.18

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	15 - 199	1.5e-44	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	234 - 448	1e-80	Peptidase family M1 domain
ERAP1_C	PF11838	538 - 856	3.3e-81	ERAP1-like C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000648)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33090
fragaria_vesca	FvH4_2g31431 FvH4_2g31440 FvH4_6g34250 FvH4_6g34262 FvH4_6g34263
malus_domestica	MD08G1066400.v1.1 MD08G1066500.v1.1 MD08G1067400.v1.1 MD09G1070300.v1.1 MD09G1187200.v1.1 MD10G1090900.v1.1 MD15G1054400.v1.1 MD17G1171400.v1.1
prunus_persica	Prupe.1G407900_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G457300_v2.0.a1 Prupe.3G044500_v2.0.a1 Prupe.3G044500_v2.0.a1
pyrus_communis	pycom08g05520 pycom08g05550 pycom08g05560 pycom08g05590 pycom09g10370 pycom09g10380 pycom10g07440 pycom10g07460 pycom15g05130 pycom15g05150 pycom17g16900
rosa_chinensis	RchiOBHm_Chr2g0144571 RchiOBHm_Chr2g0144611 RchiOBHm_Chr4g0390591 RchiOBHm_Chr6g0311371 RchiOBHm_Chr6g0311381
rosa_laevigata	RLG00000009010 RLG00000009289 RLG00000010411 RLG00000010412 RLG00000020090 RLG00000020092 RLG00000036974
rosa_multiflora	Rmu_co8212940.1_g000001 Rmu_sc0005789.1_g000001 Rmu_sc0008536.1_g000010 Rmu_sc0008536.1_g000011 Rmu_sc0009300.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056400 Rroxscaffold_2G00084720 Rroxscaffold_2G00101200 Rroxscaffold_7G00157670 Rroxscaffold_7G00157680
rosa_rugosa	Rorug02G0387900 Rorug02G0388000 Rorug02G0388100 Rorug06G0388300 Rorug06G0388400
rosa_samantha	Rh2AG440000 Rh2AG440100 Rh2BG450000 Rh2BG450300 Rh2BG450500 Rh2CG427000 Rh2CG427200 Rh2DG459900 Rh2DG460300 Rh2DG460500 Rh6AG502600 Rh6AG502700 Rh6BG513300 Rh6CG519100 Rh6CG519200
rosa_wichuraiana	Rw2G036000 Rw6G043770 Rw6G043780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1860, 2240
AasI	GACNNNNNNGTC	1 cut(s)	716
Acc36I	ACCTGC	2 cut(s)	457, 1684
AccB7I	CCANNNNNTGG	1 cut(s)	2415
AccI	GTMKAC	1 cut(s)	587
AccII	CGCG	1 cut(s)	25
AclWI	GGATC	3 cut(s)	1622, 1638, 2258
AcoI	YGGCCR	1 cut(s)	600
AcsI	RAATTY	9 cut(s)	35, 92, 731, 868, 1336, 1541, 1840, 2010, 2078
AcuI	CTGAAG	2 cut(s)	2303, 2405
AfaI	GTAC	6 cut(s)	358, 761, 836, 1890, 1940, 2277
AfiI	CCNNNNNNNGG	5 cut(s)	1114, 1759, 1973, 2320, 2415
AflII	CTTAAG	1 cut(s)	1079
AjiI	CACGTC	1 cut(s)	2379
AjnI	CCWGG	3 cut(s)	419, 1972, 2414
AjuI	GAANNNNNNNTTGG	4 cut(s)	12, 44, 689, 721
Alw21I	GWGCWC	3 cut(s)	168, 1494, 2403
Alw26I	GTCTC	6 cut(s)	112, 133, 193, 269, 1532, 2597
Alw44I	GTGCAC	1 cut(s)	1490
AlwI	GGATC	3 cut(s)	1622, 1638, 2258
Ama87I	CYCGRG	2 cut(s)	1423, 1892
AoxI	GGCC	3 cut(s)	16, 600, 807
ApaLI	GTGCAC	1 cut(s)	1490
ApeKI	GCWGC	8 cut(s)	158, 875, 878, 1133, 1292, 1551, 1877, 2327
ApoI	RAATTY	9 cut(s)	35, 92, 731, 868, 1336, 1541, 1840, 2010, 2078
AseI	ATTAAT	1 cut(s)	2052
Asp700I	GAANNNNTTC	1 cut(s)	2187
AspLEI	GCGC	1 cut(s)	925
AspS9I	GGNCC	3 cut(s)	709, 807, 2311
AsuHPI	GGTGA	8 cut(s)	1012, 1097, 1325, 1451, 1663, 1988, 2454, 2612
AsuNHI	GCTAGC	1 cut(s)	2438
AvaI	CYCGRG	2 cut(s)	1423, 1892
AvaII	GGWCC	2 cut(s)	709, 2311
BaeGI	GKGCMC	1 cut(s)	1494
BalI	TGGCCA	1 cut(s)	602
BanII	GRGCYC	1 cut(s)	168
BbsI	GAAGAC	3 cut(s)	252, 560, 2295
Bbv12I	GWGCWC	3 cut(s)	168, 1494, 2403
BbvCI	CCTCAGC	1 cut(s)	1137
BbvI	GCAGC	8 cut(s)	145, 865, 887, 1120, 1279, 1538, 1889, 2314
BccI	CCATC	7 cut(s)	119, 536, 641, 1082, 1118, 1436, 2166
BceAI	ACGGC	2 cut(s)	26, 742
BcgI	CGANNNNNNTGC	2 cut(s)	971, 1005
BciT130I	CCWGG	3 cut(s)	421, 1974, 2416
BcoDI	GTCTC	6 cut(s)	112, 133, 193, 269, 1532, 2597
BfaI	CTAG	4 cut(s)	1071, 1559, 2439, 2650
BfmI	CTRYAG	2 cut(s)	873, 1944
BfrI	CTTAAG	1 cut(s)	1079
BfuAI	ACCTGC	2 cut(s)	457, 1684
BglII	AGATCT	2 cut(s)	210, 2608
BmcAI	AGTACT	2 cut(s)	1940, 2277
Bme1390I	CCNGG	3 cut(s)	421, 1974, 2416
Bme18I	GGWCC	2 cut(s)	709, 2311
BmeT110I	CYCGRG	2 cut(s)	1423, 1892
BmgBI	CACGTC	1 cut(s)	2379
BmgT120I	GGNCC	3 cut(s)	709, 807, 2311
BmiI	GGNNCC	4 cut(s)	103, 808, 1432, 1965
BmrFI	CCNGG	3 cut(s)	421, 1974, 2416
BmrI	ACTGGG	1 cut(s)	2404
BmsI	GCATC	8 cut(s)	166, 406, 1147, 1185, 1792, 1873, 2261, 2314
BmtI	GCTAGC	1 cut(s)	2442
BmuI	ACTGGG	1 cut(s)	2404
BpiI	GAAGAC	3 cut(s)	252, 560, 2295
BplI	GAGNNNNNCTC	2 cut(s)	2390, 2422
Bpu10I	CCTNAGC	1 cut(s)	1137
BpuEI	CTTGAG	1 cut(s)	1319
BsaAI	YACGTR	1 cut(s)	834
BsaJI	CCNNGG	6 cut(s)	23, 691, 810, 1435, 2332, 2415
Bsc4I	CCNNNNNNNGG	5 cut(s)	1114, 1759, 1973, 2320, 2415
Bse118I	RCCGGY	2 cut(s)	98, 520
Bse1I	ACTGG	8 cut(s)	410, 928, 1228, 1642, 1936, 2102, 2283, 2399
Bse3DI	GCAATG	2 cut(s)	1454, 1784
BseBI	CCWGG	3 cut(s)	421, 1974, 2416
BseDI	CCNNGG	6 cut(s)	23, 691, 810, 1435, 2332, 2415
BseLI	CCNNNNNNNGG	5 cut(s)	1114, 1759, 1973, 2320, 2415
BseMI	GCAATG	2 cut(s)	1454, 1784
BseMII	CTCAG	4 cut(s)	1064, 1128, 1361, 2298
BseNI	ACTGG	8 cut(s)	410, 928, 1228, 1642, 1936, 2102, 2283, 2399
BseRI	GAGGAG	3 cut(s)	1316, 2019, 2237
BseSI	GKGCMC	1 cut(s)	1494
BseXI	GCAGC	8 cut(s)	145, 865, 887, 1120, 1279, 1538, 1889, 2314
BsgI	GTGCAG	3 cut(s)	1662, 2472, 2585
Bsh1236I	CGCG	1 cut(s)	25
BshFI	GGCC	3 cut(s)	18, 602, 809
BsiHKAI	GWGCWC	3 cut(s)	168, 1494, 2403
BsiHKCI	CYCGRG	2 cut(s)	1423, 1892
BsiSI	CCGG	2 cut(s)	99, 521
BslI	CCNNNNNNNGG	5 cut(s)	1114, 1759, 1973, 2320, 2415
BsmAI	GTCTC	6 cut(s)	112, 133, 193, 269, 1532, 2597
BsmBI	CGTCTC	3 cut(s)	112, 133, 193
BsmI	GAATGC	1 cut(s)	1226
BsnI	GGCC	3 cut(s)	18, 602, 809
BsoBI	CYCGRG	2 cut(s)	1423, 1892
Bsp1286I	GDGCHC	3 cut(s)	168, 1494, 2403
Bsp143I	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
Bsp19I	CCATGG	1 cut(s)	810
BspANI	GGCC	3 cut(s)	18, 602, 809
BspCNI	CTCAG	4 cut(s)	1063, 1129, 1360, 2299
BspFNI	CGCG	1 cut(s)	25
BspLI	GGNNCC	4 cut(s)	103, 808, 1432, 1965
BspMAI	CTGCAG	2 cut(s)	877, 1948
BspMI	ACCTGC	2 cut(s)	457, 1684
BspOI	GCTAGC	1 cut(s)	2442
BspPI	GGATC	3 cut(s)	1622, 1638, 2258
BspTI	CTTAAG	1 cut(s)	1079
BsrDI	GCAATG	2 cut(s)	1454, 1784
BsrFI	RCCGGY	2 cut(s)	98, 520
BsrI	ACTGG	8 cut(s)	410, 928, 1228, 1642, 1936, 2102, 2283, 2399
BssAI	RCCGGY	2 cut(s)	98, 520
BssECI	CCNNGG	6 cut(s)	23, 691, 810, 1435, 2332, 2415
BssMI	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
BssT1I	CCWWGG	3 cut(s)	691, 810, 1435
Bst2UI	CCWGG	3 cut(s)	421, 1974, 2416
Bst4CI	ACNGT	4 cut(s)	374, 559, 1831, 2466
Bst6I	CTCTTC	2 cut(s)	208, 2193
BstAFI	CTTAAG	1 cut(s)	1079
BstAPI	GCANNNNNTGC	2 cut(s)	1874, 1883
BstBAI	YACGTR	1 cut(s)	834
BstC8I	GCNNGC	7 cut(s)	88, 100, 1257, 1765, 1769, 1866, 2440
BstDEI	CTNAG	9 cut(s)	1050, 1137, 1347, 1682, 2000, 2083, 2192, 2216, 2307
BstDSI	CCRYGG	3 cut(s)	23, 810, 2332
BstEII	GGTNACC	1 cut(s)	1358
BstFNI	CGCG	1 cut(s)	25
BstHHI	GCGC	1 cut(s)	925
BstKTI	GATC	9 cut(s)	213, 637, 1125, 1390, 1617, 1633, 2215, 2266, 2611
BstMAI	GTCTC	6 cut(s)	112, 133, 193, 269, 1532, 2597
BstMBI	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
BstMWI	GCNNNNNNNGC	5 cut(s)	155, 458, 1090, 1874, 1883
BstNI	CCWGG	3 cut(s)	421, 1974, 2416
BstNSI	RCATGY	3 cut(s)	520, 1259, 2164
BstPI	GGTNACC	1 cut(s)	1358
BstSCI	CCNGG	3 cut(s)	419, 1972, 2414
BstSFI	CTRYAG	2 cut(s)	873, 1944
BstSLI	GKGCMC	1 cut(s)	1494
BstSNI	TACGTA	1 cut(s)	834
BstUI	CGCG	1 cut(s)	25
BstV1I	GCAGC	8 cut(s)	145, 865, 887, 1120, 1279, 1538, 1889, 2314
BstV2I	GAAGAC	3 cut(s)	252, 560, 2295
BstX2I	RGATCY	3 cut(s)	210, 2263, 2608
BstXI	CCANNNNNNTGG	2 cut(s)	935, 1442
BstYI	RGATCY	3 cut(s)	210, 2263, 2608
BsuRI	GGCC	3 cut(s)	18, 602, 809
BtgI	CCRYGG	3 cut(s)	23, 810, 2332
BtgZI	GCGATG	2 cut(s)	67, 130
BtrI	CACGTC	1 cut(s)	2379
BtsI	GCAGTG	1 cut(s)	2136
BtsIMutI	CAGTG	5 cut(s)	379, 935, 1836, 2136, 2455
BveI	ACCTGC	2 cut(s)	457, 1684
Cac8I	GCNNGC	7 cut(s)	88, 100, 1257, 1765, 1769, 1866, 2440
CfoI	GCGC	1 cut(s)	925
Cfr10I	RCCGGY	2 cut(s)	98, 520
Cfr13I	GGNCC	3 cut(s)	709, 807, 2311
Cfr42I	CCGCGG	1 cut(s)	26
CsiI	ACCWGGT	1 cut(s)	1972
Csp6I	GTAC	6 cut(s)	357, 760, 835, 1889, 1939, 2276
CviQI	GTAC	6 cut(s)	357, 760, 835, 1889, 1939, 2276
DdeI	CTNAG	9 cut(s)	1050, 1137, 1347, 1682, 2000, 2083, 2192, 2216, 2307
DpnI	GATC	9 cut(s)	212, 636, 1124, 1389, 1616, 1632, 2214, 2265, 2610
DpnII	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
DrdI	GACNNNNNNGTC	1 cut(s)	716
DseDI	GACNNNNNNGTC	1 cut(s)	716
EaeI	YGGCCR	1 cut(s)	600
Eam1104I	CTCTTC	2 cut(s)	208, 2193
EarI	CTCTTC	2 cut(s)	208, 2193
EciI	GGCGGA	1 cut(s)	2303
Ecl136II	GAGCTC	1 cut(s)	166
Eco105I	TACGTA	1 cut(s)	834
Eco130I	CCWWGG	3 cut(s)	691, 810, 1435
Eco24I	GRGCYC	1 cut(s)	168
Eco32I	GATATC	2 cut(s)	124, 1308
Eco47I	GGWCC	2 cut(s)	709, 2311
Eco53kI	GAGCTC	1 cut(s)	166
Eco57I	CTGAAG	2 cut(s)	2303, 2405
Eco88I	CYCGRG	2 cut(s)	1423, 1892
Eco91I	GGTNACC	1 cut(s)	1358
EcoICRI	GAGCTC	1 cut(s)	166
EcoO65I	GGTNACC	1 cut(s)	1358
EcoRI	GAATTC	2 cut(s)	1336, 2078
EcoRII	CCWGG	3 cut(s)	419, 1972, 2414
EcoRV	GATATC	2 cut(s)	124, 1308
EcoT14I	CCWWGG	3 cut(s)	691, 810, 1435
EcoT38I	GRGCYC	1 cut(s)	168
ErhI	CCWWGG	3 cut(s)	691, 810, 1435
Esp3I	CGTCTC	3 cut(s)	112, 133, 193
FalI	AAGNNNNNCTT	2 cut(s)	1691, 1723
FauI	CCCGC	1 cut(s)	654
FauNDI	CATATG	4 cut(s)	361, 549, 1483, 1853
FblI	GTMKAC	1 cut(s)	587
FriOI	GRGCYC	1 cut(s)	168
FspBI	CTAG	4 cut(s)	1071, 1559, 2439, 2650
GlaI	GCGC	1 cut(s)	924
HaeIII	GGCC	3 cut(s)	18, 602, 809
HapII	CCGG	2 cut(s)	99, 521
HhaI	GCGC	1 cut(s)	925
Hin6I	GCGC	1 cut(s)	923
HinP1I	GCGC	1 cut(s)	923
HincII	GTYRAC	1 cut(s)	1624
HindII	GTYRAC	1 cut(s)	1624
HindIII	AAGCTT	1 cut(s)	1952
HpaII	CCGG	2 cut(s)	99, 521
HphI	GGTGA	8 cut(s)	1012, 1097, 1325, 1451, 1663, 1988, 2454, 2612
Hpy166II	GTNNAC	7 cut(s)	588, 963, 1316, 1324, 1492, 1624, 2200
Hpy8I	GTNNAC	7 cut(s)	588, 963, 1316, 1324, 1492, 1624, 2200
Hpy99I	CGWCG	2 cut(s)	176, 245
HpyAV	CCTTC	9 cut(s)	231, 311, 334, 451, 1561, 2117, 2327, 2351, 2380
HpyCH4III	ACNGT	4 cut(s)	374, 559, 1831, 2466
HpyCH4IV	ACGT	4 cut(s)	64, 833, 1793, 2378
HpyF10VI	GCNNNNNNNGC	5 cut(s)	155, 458, 1090, 1874, 1883
HpyF3I	CTNAG	9 cut(s)	1050, 1137, 1347, 1682, 2000, 2083, 2192, 2216, 2307
HpySE526I	ACGT	4 cut(s)	64, 833, 1793, 2378
HspAI	GCGC	1 cut(s)	923
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
KspI	CCGCGG	1 cut(s)	26
Kzo9I	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
LmnI	GCTCC	6 cut(s)	4, 107, 1178, 1559, 1963, 2398
Lsp1109I	GCAGC	8 cut(s)	145, 865, 887, 1120, 1279, 1538, 1889, 2314
LweI	GCATC	8 cut(s)	166, 406, 1147, 1185, 1792, 1873, 2261, 2314
MabI	ACCWGGT	1 cut(s)	1972
MaeI	CTAG	4 cut(s)	1071, 1559, 2439, 2650
MaeII	ACGT	4 cut(s)	64, 833, 1793, 2378
MaeIII	GTNAC	7 cut(s)	394, 559, 829, 907, 946, 1230, 1358
MalI	GATC	9 cut(s)	212, 636, 1124, 1389, 1616, 1632, 2214, 2265, 2610
MboI	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
MflI	RGATCY	3 cut(s)	210, 2263, 2608
MhlI	GDGCHC	3 cut(s)	168, 1494, 2403
MlsI	TGGCCA	1 cut(s)	602
MluNI	TGGCCA	1 cut(s)	602
MlyI	GAGTC	3 cut(s)	718, 1042, 1339
MmeI	TCCRAC	4 cut(s)	505, 537, 1592, 1779
Mox20I	TGGCCA	1 cut(s)	602
MroNI	GCCGGC	1 cut(s)	98
MroXI	GAANNNNTTC	1 cut(s)	2187
MscI	TGGCCA	1 cut(s)	602
MseI	TTAA	7 cut(s)	216, 1080, 2015, 2052, 2487, 2508, 2571
MslI	CAYNNNNRTG	2 cut(s)	372, 970
Msp20I	TGGCCA	1 cut(s)	602
MspA1I	CMGCKG	3 cut(s)	25, 878, 1880
MspCI	CTTAAG	1 cut(s)	1079
MspI	CCGG	2 cut(s)	99, 521
MspR9I	CCNGG	3 cut(s)	421, 1974, 2416
Mva1269I	GAATGC	1 cut(s)	1226
MvaI	CCWGG	3 cut(s)	421, 1974, 2416
MvnI	CGCG	1 cut(s)	25
MwoI	GCNNNNNNNGC	5 cut(s)	155, 458, 1090, 1874, 1883
NaeI	GCCGGC	1 cut(s)	100
NcoI	CCATGG	1 cut(s)	810
NdeI	CATATG	4 cut(s)	361, 549, 1483, 1853
NdeII	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
NgoMIV	GCCGGC	1 cut(s)	98
NheI	GCTAGC	1 cut(s)	2438
NlaIV	GGNNCC	4 cut(s)	103, 808, 1432, 1965
NmeAIII	GCCGAG	1 cut(s)	187
NmuCI	GTSAC	2 cut(s)	907, 1230
NspI	RCATGY	3 cut(s)	520, 1259, 2164
PaeI	GCATGC	1 cut(s)	1259
PaeR7I	CTCGAG	1 cut(s)	1423
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	1 cut(s)	1226
PdiI	GCCGGC	1 cut(s)	100
PdmI	GAANNNNTTC	1 cut(s)	2187
PflMI	CCANNNNNTGG	1 cut(s)	2415
PleI	GAGTC	3 cut(s)	718, 1042, 1339
PpsI	GAGTC	3 cut(s)	718, 1042, 1339
Ppu21I	YACGTR	1 cut(s)	834
PshBI	ATTAAT	1 cut(s)	2052
PsiI	TTATAA	2 cut(s)	1860, 2240
Psp124BI	GAGCTC	1 cut(s)	168
Psp6I	CCWGG	3 cut(s)	419, 1972, 2414
PspEI	GGTNACC	1 cut(s)	1358
PspGI	CCWGG	3 cut(s)	419, 1972, 2414
PspN4I	GGNNCC	4 cut(s)	103, 808, 1432, 1965
PspPI	GGNCC	3 cut(s)	709, 807, 2311
PspXI	VCTCGAGB	1 cut(s)	1423
PsrI	GAACNNNNNNTAC	2 cut(s)	2268, 2300
PstI	CTGCAG	2 cut(s)	877, 1948
PsuI	RGATCY	3 cut(s)	210, 2263, 2608
PvuII	CAGCTG	2 cut(s)	878, 1880
RsaI	GTAC	6 cut(s)	358, 761, 836, 1890, 1940, 2277
RsaNI	GTAC	6 cut(s)	357, 760, 835, 1889, 1939, 2276
RseI	CAYNNNNRTG	2 cut(s)	372, 970
SacI	GAGCTC	1 cut(s)	168
SacII	CCGCGG	1 cut(s)	26
SaqAI	TTAA	7 cut(s)	216, 1080, 2015, 2052, 2487, 2508, 2571
Sau3AI	GATC	9 cut(s)	210, 634, 1122, 1387, 1614, 1630, 2212, 2263, 2608
Sau96I	GGNCC	3 cut(s)	709, 807, 2311
ScaI	AGTACT	2 cut(s)	1940, 2277
SchI	GAGTC	3 cut(s)	718, 1042, 1339
ScrFI	CCNGG	3 cut(s)	421, 1974, 2416
SduI	GDGCHC	3 cut(s)	168, 1494, 2403
SexAI	ACCWGGT	1 cut(s)	1972
SfaNI	GCATC	8 cut(s)	166, 406, 1147, 1185, 1792, 1873, 2261, 2314
SfcI	CTRYAG	2 cut(s)	873, 1944
Sfr274I	CTCGAG	1 cut(s)	1423
Sfr303I	CCGCGG	1 cut(s)	26
SgrBI	CCGCGG	1 cut(s)	26
SinI	GGWCC	2 cut(s)	709, 2311
SlaI	CTCGAG	1 cut(s)	1423
SmiMI	CAYNNNNRTG	2 cut(s)	372, 970
SmlI	CTYRAG	3 cut(s)	1079, 1298, 1423
SmoI	CTYRAG	3 cut(s)	1079, 1298, 1423
SnaBI	TACGTA	1 cut(s)	834
SphI	GCATGC	1 cut(s)	1259
SspI	AATATT	1 cut(s)	1152
SspMI	CTAG	4 cut(s)	1071, 1559, 2439, 2650
SstI	GAGCTC	1 cut(s)	168
StyD4I	CCNGG	3 cut(s)	419, 1972, 2414
StyI	CCWWGG	3 cut(s)	691, 810, 1435
TaaI	ACNGT	4 cut(s)	374, 559, 1831, 2466
TaiI	ACGT	4 cut(s)	67, 836, 1796, 2381
TaqI	TCGA	9 cut(s)	114, 120, 139, 174, 240, 1113, 1424, 1530, 2528
TatI	WGTACW	3 cut(s)	356, 1938, 2275
TauI	GCSGC	2 cut(s)	28, 182
Tru1I	TTAA	7 cut(s)	216, 1080, 2015, 2052, 2487, 2508, 2571
Tru9I	TTAA	7 cut(s)	216, 1080, 2015, 2052, 2487, 2508, 2571
TscAI	CASTG	5 cut(s)	379, 935, 1836, 2136, 2455
TseFI	GTSAC	2 cut(s)	907, 1230
TseI	GCWGC	8 cut(s)	158, 875, 878, 1133, 1292, 1551, 1877, 2327
Tsp45I	GTSAC	2 cut(s)	907, 1230
TspGWI	ACGGA	5 cut(s)	94, 160, 175, 480, 1743
TspRI	CASTG	5 cut(s)	379, 935, 1836, 2136, 2455
Van91I	CCANNNNNTGG	1 cut(s)	2415
Vha464I	CTTAAG	1 cut(s)	1079
VneI	GTGCAC	1 cut(s)	1490
VpaK11BI	GGWCC	2 cut(s)	709, 2311
VspI	ATTAAT	1 cut(s)	2052
XapI	RAATTY	9 cut(s)	35, 92, 731, 868, 1336, 1541, 1840, 2010, 2078
XceI	RCATGY	3 cut(s)	520, 1259, 2164
XcmI	CCANNNNNNNNNTGG	2 cut(s)	778, 889
XhoI	CTCGAG	1 cut(s)	1423
XmiI	GTMKAC	1 cut(s)	587
XmnI	GAANNNNTTC	1 cut(s)	2187
XspI	CTAG	4 cut(s)	1071, 1559, 2439, 2650
ZrmI	AGTACT	2 cut(s)	1940, 2277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.