Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000648

Physical Location & Seq

Genomic Coordinates Forward (+)

3708654 .. 3714059

Gene Structure 5406 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1054400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGATGAGTCCCACTCGCACGCATTCGAAATCTGAACTCGAAAAACTCTCTCATCCCTCGCGAGTATCACACTCGTCGTCGCTTGCTATGGAGCAGTTCAAAGGCCAGCCCCGGCTTCCGAAATTCGCCGTCCCGAAACGGTACGACGTGAAGCTGAAGCCTGACCTCGCCGCCTGCAAATTCGGCGGCTCCGTCGCCATCGGCCTCGATATCGTCGCCGATACCAAATTCGTCGTCCTCAATGCCGCCGAGCTCACCGTCAACGCCGGTTCCGTCTCTTTCACTCACGGAGGCTCGTCCAAGGTGTTCAAGCCTTCGAAAGCTGAGACGTTCGAAGAGGATGGGATTTTGGTTTTGGAGTTTGGAGAGATGCTTCCGATTGGGTCTGGAGTTTTGGCTATTGAGTTTGAGGGAATTTTGAATGACAAGATGAAGGGGTTCTATAGAAGTACATATGAGTACAACGGTGAGAAGAAAAATATGGCAGTTACACAGTTTGAACCGGTTGATGCCAGGCGGTGCTTTCCATGTTGGGATGAACCTGCTTGCAAGGCTACATTCAAGATTACACTTGATGGTGTTCCGTCTGAACTAGTAGCCCTTTCCAACATGCCGATTGAAGAAGAAAAAGTTAATGGACATCTGAAGACAGTTTCATACGTAGAAACACCAATTATGTCTACATATTTGGTGGCCGTTGTTGTTGGATTGTTTGATTATGTTGAAGATCATACTTCTGATGGGGTCAAAGTGCGGGTATATTGTCAAGTTGGTAAGGCAGACCAAGGGAAATTTGCTTTGCACGTTGCTGTCAAGACACTTGAATTGTACAAAGATTACTTTGCTGTGCCATACCCTTTGCCCAAATTGGATATGGTTGCAATACCTGATTTCTCTGCTGGTGCCATGGAGAACTATGGCTTAGTTACATACCGAGAAACAGCTTTGCTTTTTGATGAACAACATTCTGCAGCTGTCAACAAGCAAAGGGTTGCGACTGTCGTGGCACATGAATTGGCACACCAGTGGTTTGGCAATCTTGTAACAATGGAATGGTGGACACATTTATGGCTGAATGAGGGATTTGCAACATGGGTAAGCTATTTGGCTACTGATAGCTTGTTCCCAGAGTGGAAAATATGGACTCAGTTTCTTGATGAACTTACTGATGGTCTTAAGCTGGATGGGCTTGAAGAATCTCACCCCATTGAGGTGGAGATCAATCATGCTGCTGAGGTTGATGAAATATTTGATGCAATAAGTTATAGAAAAGGTGCTTCTGTTATTCGGATGCTACAAAGCTATCTAGGTGCTGAAGTTTTCCAGCGGTCACTTGCTTCATATATAAAAAAGCATGCTTACTCAAATGCAAAGACAGAAGATTTGTGGGCTGCCCTTGAAGAGGGATCTGGTGAACCTGTAAACAAGCTAATGAATTCATGGACACAGCAAAAAGGTTATCCGGTTATTTCTGTCAAAGTTAAAGATCAGAAATTGGAGTTTGATCAGACACAATTCTATTCAAGTGGTTCCCAAGGCGATGGGCAATGGATTGTGCCGATCACATTATCCTGTGGCTCATATGATGTACGCAAGAATTTCTTACTACAAGCGAAGTCTGAAACTCTTGACATAAAGGAATTTCTGGGTTGCTCGGTAGGAAAGGTTGGATGTGGAGGCAACAAGGACAATGCAATATGCAGTTGGATAAAGGTCAATGTGGACCAGACTGGTTTTTACAGAGTGAAATATGAGGACGAACTTGCAGCTGCACTTAGAAACGCTATAGAAAAGAAACAATTGTCTGAAACTGACAGATTTGGCATTTTGGATGATTCATTTGCCCTATCAATGGCTCGCAAGCAGTCTTTTGCTTCATTGCTTACCTTGTTGAGTGCTTACAGAGAGGAACTTGACTATATCGTGCTGTCCAATTTGATTACTGTAAGTTATAAGCTCGCAAGAATTGCAGCTGATGCTGTACCCGGACTACTGGATCACATTAAGCAATTCTTTATTGGCCTTTTCCAGTATTCTGCTGAGAAGCTTGGTTGGCAGCCTAAACCCGGCGAAAGCCATTTAGATGCAATGTTGAGAGGAGAAATTTTGAATGTGCTTGCTGTGTTTGGACATGACCTGACATTAGATGAAGCAACTAGGCGTTTTCATGCCTTTTTAGAAGACAGAAACACGCCCCTCCTTCCCCCTGACATTAGAAAGGCAGTATATGTGGCTGTAATGCAAAGGGCAAGCGTATCTAACCAATCAAGCTATGAATCTCTTCTTAGACTCTACAGAGAGAGTGATTTAAGCCAGGAGAAGACCCGCATTCTAAGTTCTTTAGCATCATGTCCTGATCCCAATATTACATTGGAAGTTCTCAACTTTATATTGACTCCTGAGGTTCGCAGTCAGGATGCTGTCTTTGGACTTGCTGTTAGCAGTAAAGGACGGGACACAGCTTGGACGTGGATGAAGGAGAACTGGGAGCACATCTCAAAGACCTGGGGGTCTGGATTTCTAATTACTCGCTTTGTCAGTGCAATTGTTTCTCCGTTTGCTTCATTTGACAGGGTTAAGGAAATAGAGGAGTTCTTCAAAGCCCACCCCAACCCGGCGATAACCAGAACCTTGAAGCAGAGCATTGAGCGGGTACAGATTAACGCCAAGTGGGTTCAGAGCGTCCAGAGCGAGAAAAACCTTGCCGATGTTGTGACGGAGTTGGCCTACAGGAAATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006518 GO:0006807 GO:0006810 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009628 GO:0009914 GO:0009926 GO:0009987 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0031090 GO:0031406 GO:0033218 GO:0033293 GO:0033554 GO:0034641 GO:0036094 GO:0036293 GO:0036294 GO:0042221 GO:0042277 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043177 GO:0043226 GO:0043227 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060918 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ERAP1_C Peptidase_M1

Protein Analysis

914

Amino Acids

102.5

Weight (kDa)

5.69

Isoelectric Point (pI)

35.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	44 - 230	1.7e-47	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	265 - 481	1e-90	Peptidase family M1 domain
ERAP1_C	PF11838	569 - 886	1.1e-84	ERAP1-like C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000648)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33090
fragaria_vesca	FvH4_2g31431 FvH4_2g31440 FvH4_6g34250 FvH4_6g34262 FvH4_6g34263
malus_domestica	MD08G1066400.v1.1 MD08G1066500.v1.1 MD08G1067400.v1.1 MD09G1070300.v1.1 MD09G1187200.v1.1 MD10G1090900.v1.1 MD15G1054400.v1.1 MD17G1171400.v1.1
prunus_persica	Prupe.1G407900_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G457300_v2.0.a1 Prupe.3G044500_v2.0.a1 Prupe.3G044500_v2.0.a1
pyrus_communis	pycom08g05520 pycom08g05550 pycom08g05560 pycom08g05590 pycom09g10370 pycom09g10380 pycom10g07440 pycom10g07460 pycom15g05130 pycom15g05150 pycom17g16900
rosa_chinensis	RchiOBHm_Chr2g0144571 RchiOBHm_Chr2g0144611 RchiOBHm_Chr4g0390591 RchiOBHm_Chr6g0311371 RchiOBHm_Chr6g0311381
rosa_laevigata	RLG00000009010 RLG00000009289 RLG00000010411 RLG00000010412 RLG00000020090 RLG00000020092 RLG00000036974
rosa_multiflora	Rmu_co8212940.1_g000001 Rmu_sc0005789.1_g000001 Rmu_sc0008536.1_g000010 Rmu_sc0008536.1_g000011 Rmu_sc0009300.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056400 Rroxscaffold_2G00084720 Rroxscaffold_2G00101200 Rroxscaffold_7G00157670 Rroxscaffold_7G00157680
rosa_rugosa	Rorug02G0387900 Rorug02G0388000 Rorug02G0388100 Rorug06G0388300 Rorug06G0388400
rosa_samantha	Rh2AG440000 Rh2AG440100 Rh2BG450000 Rh2BG450300 Rh2BG450500 Rh2CG427000 Rh2CG427200 Rh2DG459900 Rh2DG460300 Rh2DG460500 Rh6AG502600 Rh6AG502700 Rh6BG513300 Rh6CG519100 Rh6CG519200
rosa_wichuraiana	Rw2G036000 Rw6G043770 Rw6G043780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1953
AasI	GACNNNNNNGTC	1 cut(s)	2509
Acc36I	ACCTGC	1 cut(s)	550
AccB1I	GGYRCC	1 cut(s)	902
AccBSI	CCGCTC	1 cut(s)	2650
AccI	GTMKAC	1 cut(s)	680
AccII	CGCG	1 cut(s)	61
AciI	CCGC	8 cut(s)	171, 186, 246, 517, 754, 1327, 2326, 2650
AclWI	GGATC	3 cut(s)	1414, 2004, 2351
AcoI	YGGCCR	1 cut(s)	693
AcsI	RAATTY	9 cut(s)	122, 179, 227, 414, 791, 1435, 1597, 1640, 2103
AcuI	CTGAAG	3 cut(s)	176, 665, 1335
AfaI	GTAC	7 cut(s)	143, 451, 461, 830, 1590, 1983, 2655
AfiI	CCNNNNNNNGG	5 cut(s)	1210, 1402, 1852, 2066, 2614
AflII	CTTAAG	1 cut(s)	1175
AgeI	ACCGGT	1 cut(s)	502
AhlI	ACTAGT	1 cut(s)	592
AjiI	CACGTC	2 cut(s)	148, 2469
AjnI	CCWGG	3 cut(s)	512, 2313, 2504
AleI	CACNNNNGTG	1 cut(s)	1024
Alw21I	GWGCWC	2 cut(s)	255, 2493
Alw26I	GTCTC	2 cut(s)	280, 320
AlwI	GGATC	3 cut(s)	1414, 2004, 2351
AoxI	GGCC	5 cut(s)	103, 202, 693, 2020, 2724
ApeKI	GCWGC	7 cut(s)	971, 1229, 1391, 1766, 1769, 1970, 2056
ApoI	RAATTY	9 cut(s)	122, 179, 227, 414, 791, 1435, 1597, 1640, 2103
ArsI	GACNNNNNNTTYG	4 cut(s)	219, 251, 1366, 1398
AsiGI	ACCGGT	1 cut(s)	502
Asp700I	GAANNNNTTC	2 cut(s)	437, 2280
AspS9I	GGNCC	1 cut(s)	1723
AsuC2I	CCSGG	4 cut(s)	112, 1986, 2067, 2615
AsuHPI	GGTGA	4 cut(s)	247, 479, 1193, 1424
AsuII	TTCGAA	3 cut(s)	26, 317, 333
AvaII	GGWCC	1 cut(s)	1723
AxyI	CCTNAGG	1 cut(s)	2400
BanI	GGYRCC	1 cut(s)	902
BanII	GRGCYC	1 cut(s)	255
BbsI	GAAGAC	3 cut(s)	653, 2187, 2327
Bbv12I	GWGCWC	2 cut(s)	255, 2493
BbvCI	CCTCAGC	1 cut(s)	1233
BbvI	GCAGC	7 cut(s)	983, 1216, 1378, 1756, 1778, 1982, 2068
BccI	CCATC	7 cut(s)	206, 335, 569, 734, 1163, 1178, 1535
BceAI	ACGGC	2 cut(s)	113, 680
BciT130I	CCWGG	3 cut(s)	514, 2315, 2506
BclI	TGATCA	1 cut(s)	1504
BcnI	CCSGG	4 cut(s)	112, 1986, 2067, 2615
BcoDI	GTCTC	2 cut(s)	280, 320
BcuI	ACTAGT	1 cut(s)	592
BfaI	CTAG	4 cut(s)	593, 1307, 2157, 2740
BfmI	CTRYAG	5 cut(s)	442, 969, 1785, 2293, 2728
BfrI	CTTAAG	1 cut(s)	1175
BfuAI	ACCTGC	1 cut(s)	550
Bme1390I	CCNGG	7 cut(s)	112, 514, 1986, 2067, 2315, 2506, 2615
Bme18I	GGWCC	1 cut(s)	1723
BmgBI	CACGTC	2 cut(s)	148, 2469
BmgT120I	GGNCC	1 cut(s)	1723
BmiI	GGNNCC	4 cut(s)	190, 271, 904, 1531
BmrFI	CCNGG	7 cut(s)	112, 514, 1986, 2067, 2315, 2506, 2615
BmrI	ACTGGG	1 cut(s)	2494
BmsI	GCATC	8 cut(s)	360, 499, 1243, 1281, 1966, 2074, 2354, 2407
BmuI	ACTGGG	1 cut(s)	2494
BpiI	GAAGAC	3 cut(s)	653, 2187, 2327
BplI	GAGNNNNNCTC	3 cut(s)	30, 2480, 2512
BpmI	CTGGAG	1 cut(s)	408
Bpu10I	CCTNAGC	1 cut(s)	1233
Bpu14I	TTCGAA	3 cut(s)	26, 317, 333
BpuMI	CCSGG	4 cut(s)	112, 1986, 2067, 2615
BsaAI	YACGTR	1 cut(s)	661
BsaJI	CCNNGG	6 cut(s)	110, 300, 784, 906, 1534, 2505
BsaWI	WCCGGW	2 cut(s)	502, 1462
Bsc4I	CCNNNNNNNGG	5 cut(s)	1210, 1402, 1852, 2066, 2614
Bse118I	RCCGGY	2 cut(s)	266, 502
Bse1I	ACTGG	5 cut(s)	1024, 1735, 1998, 2029, 2489
Bse21I	CCTNAGG	1 cut(s)	2400
Bse3DI	GCAATG	3 cut(s)	1553, 1877, 2094
BseBI	CCWGG	3 cut(s)	514, 2315, 2506
BseDI	CCNNGG	6 cut(s)	110, 300, 784, 906, 1534, 2505
BseGI	GGATG	9 cut(s)	52, 346, 541, 1189, 1296, 1676, 1837, 2422, 2478
BseLI	CCNNNNNNNGG	5 cut(s)	1210, 1402, 1852, 2066, 2614
BseMI	GCAATG	3 cut(s)	1553, 1877, 2094
BseMII	CTCAG	5 cut(s)	315, 1160, 1224, 2031, 2391
BseNI	ACTGG	5 cut(s)	1024, 1735, 1998, 2029, 2489
BseRI	GAGGAG	2 cut(s)	2112, 2603
BseXI	GCAGC	7 cut(s)	983, 1216, 1378, 1756, 1778, 1982, 2068
BsgI	GTGCAG	1 cut(s)	1755
Bsh1236I	CGCG	1 cut(s)	61
BshFI	GGCC	5 cut(s)	105, 204, 695, 2022, 2726
BshNI	GGYRCC	1 cut(s)	902
BshTI	ACCGGT	1 cut(s)	502
BsiHKAI	GWGCWC	2 cut(s)	255, 2493
BsiSI	CCGG	7 cut(s)	112, 267, 503, 1463, 1986, 2067, 2615
BslFI	GGGAC	2 cut(s)	116, 2468
BslI	CCNNNNNNNGG	5 cut(s)	1210, 1402, 1852, 2066, 2614
BsmAI	GTCTC	2 cut(s)	280, 320
BsmBI	CGTCTC	2 cut(s)	280, 320
BsmFI	GGGAC	2 cut(s)	116, 2468
BsmI	GAATGC	2 cut(s)	22, 2328
BsnI	GGCC	5 cut(s)	105, 204, 695, 2022, 2726
Bsp119I	TTCGAA	3 cut(s)	26, 317, 333
Bsp1286I	GDGCHC	2 cut(s)	255, 2493
Bsp1407I	TGTACA	1 cut(s)	828
Bsp143I	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
Bsp19I	CCATGG	1 cut(s)	906
Bsp68I	TCGCGA	1 cut(s)	61
BspACI	CCGC	8 cut(s)	171, 186, 246, 517, 754, 1327, 2326, 2650
BspANI	GGCC	5 cut(s)	105, 204, 695, 2022, 2726
BspCNI	CTCAG	5 cut(s)	316, 1159, 1225, 2032, 2392
BspFNI	CGCG	1 cut(s)	61
BspLI	GGNNCC	4 cut(s)	190, 271, 904, 1531
BspMAI	CTGCAG	1 cut(s)	973
BspMI	ACCTGC	1 cut(s)	550
BspPI	GGATC	3 cut(s)	1414, 2004, 2351
BspT104I	TTCGAA	3 cut(s)	26, 317, 333
BspT107I	GGYRCC	1 cut(s)	902
BspTI	CTTAAG	1 cut(s)	1175
BsrBI	CCGCTC	1 cut(s)	2650
BsrDI	GCAATG	3 cut(s)	1553, 1877, 2094
BsrFI	RCCGGY	2 cut(s)	266, 502
BsrGI	TGTACA	1 cut(s)	828
BsrI	ACTGG	5 cut(s)	1024, 1735, 1998, 2029, 2489
BssAI	RCCGGY	2 cut(s)	266, 502
BssECI	CCNNGG	6 cut(s)	110, 300, 784, 906, 1534, 2505
BssMI	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
BssT1I	CCWWGG	4 cut(s)	300, 784, 906, 1534
Bst2UI	CCWGG	3 cut(s)	514, 2315, 2506
Bst4CI	ACNGT	7 cut(s)	141, 259, 467, 495, 652, 1000, 1945
Bst6I	CTCTTC	3 cut(s)	330, 1395, 2286
BstAFI	CTTAAG	1 cut(s)	1175
BstAPI	GCANNNNNTGC	2 cut(s)	1967, 1976
BstAUI	TGTACA	1 cut(s)	828
BstBAI	YACGTR	1 cut(s)	661
BstBI	TTCGAA	3 cut(s)	26, 317, 333
BstDEI	CTNAG	9 cut(s)	324, 922, 1146, 1233, 1775, 2040, 2285, 2333, 2400
BstDSI	CCRYGG	1 cut(s)	906
BstENI	CCTNNNNNAGG	1 cut(s)	1400
BstF5I	GGATG	9 cut(s)	52, 346, 541, 1189, 1296, 1676, 1837, 2422, 2478
BstFNI	CGCG	1 cut(s)	61
BstKTI	GATC	8 cut(s)	730, 1221, 1409, 1489, 1507, 1563, 1999, 2359
BstMAI	GTCTC	2 cut(s)	280, 320
BstMBI	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
BstMWI	GCNNNNNNNGC	7 cut(s)	183, 551, 1186, 1967, 1976, 2053, 2688
BstNI	CCWGG	3 cut(s)	514, 2315, 2506
BstNSI	RCATGY	2 cut(s)	613, 1358
BstSCI	CCNGG	7 cut(s)	110, 512, 1984, 2065, 2313, 2504, 2613
BstSFI	CTRYAG	5 cut(s)	442, 969, 1785, 2293, 2728
BstSNI	TACGTA	1 cut(s)	661
BstUI	CGCG	1 cut(s)	61
BstV1I	GCAGC	7 cut(s)	983, 1216, 1378, 1756, 1778, 1982, 2068
BstV2I	GAAGAC	3 cut(s)	653, 2187, 2327
BstX2I	RGATCY	1 cut(s)	1406
BstXI	CCANNNNNNTGG	3 cut(s)	1031, 1213, 1541
BstYI	RGATCY	1 cut(s)	1406
Bsu36I	CCTNAGG	1 cut(s)	2400
BsuRI	GGCC	5 cut(s)	105, 204, 695, 2022, 2726
BtgI	CCRYGG	1 cut(s)	906
BtgZI	GCGATG	1 cut(s)	1554
BtrI	CACGTC	2 cut(s)	148, 2469
BtsCI	GGATG	9 cut(s)	52, 346, 541, 1189, 1296, 1676, 1837, 2422, 2478
BtsIMutI	CAGTG	2 cut(s)	1031, 2545
BtuMI	TCGCGA	1 cut(s)	61
BveI	ACCTGC	1 cut(s)	550
Cfr10I	RCCGGY	2 cut(s)	266, 502
Cfr13I	GGNCC	1 cut(s)	1723
CseI	GACGC	1 cut(s)	2671
Csp6I	GTAC	7 cut(s)	142, 450, 460, 829, 1589, 1982, 2654
CspAI	ACCGGT	1 cut(s)	502
CviQI	GTAC	7 cut(s)	142, 450, 460, 829, 1589, 1982, 2654
DdeI	CTNAG	9 cut(s)	324, 922, 1146, 1233, 1775, 2040, 2285, 2333, 2400
DpnI	GATC	8 cut(s)	729, 1220, 1408, 1488, 1506, 1562, 1998, 2358
DpnII	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
DrdI	GACNNNNNNGTC	1 cut(s)	2509
DseDI	GACNNNNNNGTC	1 cut(s)	2509
EaeI	YGGCCR	1 cut(s)	693
Eam1104I	CTCTTC	3 cut(s)	330, 1395, 2286
EarI	CTCTTC	3 cut(s)	330, 1395, 2286
Ecl136II	GAGCTC	1 cut(s)	253
Eco105I	TACGTA	1 cut(s)	661
Eco130I	CCWWGG	4 cut(s)	300, 784, 906, 1534
Eco24I	GRGCYC	1 cut(s)	255
Eco32I	GATATC	1 cut(s)	211
Eco47I	GGWCC	1 cut(s)	1723
Eco53kI	GAGCTC	1 cut(s)	253
Eco57I	CTGAAG	3 cut(s)	176, 665, 1335
Eco81I	CCTNAGG	1 cut(s)	2400
EcoICRI	GAGCTC	1 cut(s)	253
EcoNI	CCTNNNNNAGG	1 cut(s)	1400
EcoRI	GAATTC	1 cut(s)	1435
EcoRII	CCWGG	3 cut(s)	512, 2313, 2504
EcoRV	GATATC	1 cut(s)	211
EcoT14I	CCWWGG	4 cut(s)	300, 784, 906, 1534
EcoT38I	GRGCYC	1 cut(s)	255
ErhI	CCWWGG	4 cut(s)	300, 784, 906, 1534
Esp3I	CGTCTC	2 cut(s)	280, 320
FalI	AAGNNNNNCTT	4 cut(s)	1586, 1618, 1997, 2029
FaqI	GGGAC	2 cut(s)	116, 2468
FauI	CCCGC	3 cut(s)	747, 2333, 2643
FauNDI	CATATG	2 cut(s)	454, 1582
FbaI	TGATCA	1 cut(s)	1504
FblI	GTMKAC	1 cut(s)	680
FokI	GGATG	9 cut(s)	39, 353, 548, 1196, 1303, 1683, 1844, 2429, 2485
FriOI	GRGCYC	1 cut(s)	255
FspBI	CTAG	4 cut(s)	593, 1307, 2157, 2740
GsuI	CTGGAG	1 cut(s)	408
HaeIII	GGCC	5 cut(s)	105, 204, 695, 2022, 2726
HapII	CCGG	7 cut(s)	112, 267, 503, 1463, 1986, 2067, 2615
HgaI	GACGC	1 cut(s)	2671
HincII	GTYRAC	2 cut(s)	262, 979
HindII	GTYRAC	2 cut(s)	262, 979
HindIII	AAGCTT	1 cut(s)	2045
HinfI	GANTC	7 cut(s)	7, 1144, 1196, 1835, 2276, 2289, 2395
HpaII	CCGG	7 cut(s)	112, 267, 503, 1463, 1986, 2067, 2615
HphI	GGTGA	4 cut(s)	247, 479, 1193, 1424
Hpy166II	GTNNAC	7 cut(s)	262, 681, 979, 1059, 1415, 1423, 1723
Hpy8I	GTNNAC	7 cut(s)	262, 681, 979, 1059, 1415, 1423, 1723
Hpy99I	CGWCG	6 cut(s)	79, 82, 149, 197, 218, 236
HpyAV	CCTTC	4 cut(s)	324, 427, 2210, 2470
HpyCH4III	ACNGT	7 cut(s)	141, 259, 467, 495, 652, 1000, 1945
HpyCH4IV	ACGT	5 cut(s)	147, 329, 660, 804, 2468
HpyF10VI	GCNNNNNNNGC	7 cut(s)	183, 551, 1186, 1967, 1976, 2053, 2688
HpyF3I	CTNAG	9 cut(s)	324, 922, 1146, 1233, 1775, 2040, 2285, 2333, 2400
HpySE526I	ACGT	5 cut(s)	147, 329, 660, 804, 2468
Ksp22I	TGATCA	1 cut(s)	1504
Kzo9I	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
LmnI	GCTCC	3 cut(s)	91, 194, 2488
Lsp1109I	GCAGC	7 cut(s)	983, 1216, 1378, 1756, 1778, 1982, 2068
LweI	GCATC	8 cut(s)	360, 499, 1243, 1281, 1966, 2074, 2354, 2407
MaeI	CTAG	4 cut(s)	593, 1307, 2157, 2740
MaeII	ACGT	5 cut(s)	147, 329, 660, 804, 2468
MaeIII	GTNAC	5 cut(s)	487, 925, 1042, 1329, 2713
MalI	GATC	8 cut(s)	729, 1220, 1408, 1488, 1506, 1562, 1998, 2358
MbiI	CCGCTC	1 cut(s)	2650
MboI	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
MfeI	CAATTG	2 cut(s)	1799, 2544
MflI	RGATCY	1 cut(s)	1406
MhlI	GDGCHC	2 cut(s)	255, 2493
MlyI	GAGTC	4 cut(s)	16, 1138, 2283, 2389
MmeI	TCCRAC	4 cut(s)	630, 685, 1648, 1685
MroXI	GAANNNNTTC	2 cut(s)	437, 2280
MseI	TTAA	7 cut(s)	633, 1176, 1482, 2004, 2309, 2577, 2661
MslI	CAYNNNNRTG	4 cut(s)	1024, 1066, 1211, 2082
MspA1I	CMGCKG	4 cut(s)	974, 1327, 1769, 1973
MspCI	CTTAAG	1 cut(s)	1175
MspI	CCGG	7 cut(s)	112, 267, 503, 1463, 1986, 2067, 2615
MspR9I	CCNGG	7 cut(s)	112, 514, 1986, 2067, 2315, 2506, 2615
MunI	CAATTG	2 cut(s)	1799, 2544
Mva1269I	GAATGC	2 cut(s)	22, 2328
MvaI	CCWGG	3 cut(s)	514, 2315, 2506
MvnI	CGCG	1 cut(s)	61
MwoI	GCNNNNNNNGC	7 cut(s)	183, 551, 1186, 1967, 1976, 2053, 2688
NciI	CCSGG	4 cut(s)	112, 1986, 2067, 2615
NcoI	CCATGG	1 cut(s)	906
NdeI	CATATG	2 cut(s)	454, 1582
NdeII	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
NlaIV	GGNNCC	4 cut(s)	190, 271, 904, 1531
NmeAIII	GCCGAG	1 cut(s)	274
NmuCI	GTSAC	2 cut(s)	1329, 2713
NruI	TCGCGA	1 cut(s)	61
NspI	RCATGY	2 cut(s)	613, 1358
NspV	TTCGAA	3 cut(s)	26, 317, 333
OliI	CACNNNNGTG	1 cut(s)	1024
PaeI	GCATGC	1 cut(s)	1358
PcsI	WCGNNNNNNNCGW	2 cut(s)	189, 270
PctI	GAATGC	2 cut(s)	22, 2328
PdmI	GAANNNNTTC	2 cut(s)	437, 2280
PfeI	GAWTC	3 cut(s)	1196, 1835, 2276
PinAI	ACCGGT	1 cut(s)	502
PleI	GAGTC	4 cut(s)	15, 1138, 2283, 2389
PpsI	GAGTC	4 cut(s)	15, 1138, 2283, 2389
Ppu21I	YACGTR	1 cut(s)	661
PsiI	TTATAA	1 cut(s)	1953
Psp124BI	GAGCTC	1 cut(s)	255
Psp6I	CCWGG	3 cut(s)	512, 2313, 2504
PspGI	CCWGG	3 cut(s)	512, 2313, 2504
PspN4I	GGNNCC	4 cut(s)	190, 271, 904, 1531
PspPI	GGNCC	1 cut(s)	1723
PstI	CTGCAG	1 cut(s)	973
PsuI	RGATCY	1 cut(s)	1406
PvuII	CAGCTG	3 cut(s)	974, 1769, 1973
RruI	TCGCGA	1 cut(s)	61
RsaI	GTAC	7 cut(s)	143, 451, 461, 830, 1590, 1983, 2655
RsaNI	GTAC	7 cut(s)	142, 450, 460, 829, 1589, 1982, 2654
RseI	CAYNNNNRTG	4 cut(s)	1024, 1066, 1211, 2082
SacI	GAGCTC	1 cut(s)	255
SaqAI	TTAA	7 cut(s)	633, 1176, 1482, 2004, 2309, 2577, 2661
Sau3AI	GATC	8 cut(s)	727, 1218, 1406, 1486, 1504, 1560, 1996, 2356
Sau96I	GGNCC	1 cut(s)	1723
SchI	GAGTC	4 cut(s)	16, 1138, 2283, 2389
ScrFI	CCNGG	7 cut(s)	112, 514, 1986, 2067, 2315, 2506, 2615
SduI	GDGCHC	2 cut(s)	255, 2493
SfaNI	GCATC	8 cut(s)	360, 499, 1243, 1281, 1966, 2074, 2354, 2407
SfcI	CTRYAG	5 cut(s)	442, 969, 1785, 2293, 2728
SfuI	TTCGAA	3 cut(s)	26, 317, 333
SinI	GGWCC	1 cut(s)	1723
SmiMI	CAYNNNNRTG	4 cut(s)	1024, 1066, 1211, 2082
SmlI	CTYRAG	1 cut(s)	1175
SmoI	CTYRAG	1 cut(s)	1175
SnaBI	TACGTA	1 cut(s)	661
SpeI	ACTAGT	1 cut(s)	592
SphI	GCATGC	1 cut(s)	1358
SsiI	CCGC	8 cut(s)	171, 186, 246, 517, 754, 1327, 2326, 2650
SspI	AATATT	2 cut(s)	1248, 2365
SspMI	CTAG	4 cut(s)	593, 1307, 2157, 2740
SstI	GAGCTC	1 cut(s)	255
StyD4I	CCNGG	7 cut(s)	110, 512, 1984, 2065, 2313, 2504, 2613
StyI	CCWWGG	4 cut(s)	300, 784, 906, 1534
TaaI	ACNGT	7 cut(s)	141, 259, 467, 495, 652, 1000, 1945
TaiI	ACGT	5 cut(s)	150, 332, 663, 807, 2471
TaqI	TCGA	5 cut(s)	26, 39, 207, 317, 333
TatI	WGTACW	3 cut(s)	449, 459, 828
TauI	GCSGC	3 cut(s)	173, 189, 248
TfiI	GAWTC	3 cut(s)	1196, 1835, 2276
Tru1I	TTAA	7 cut(s)	633, 1176, 1482, 2004, 2309, 2577, 2661
Tru9I	TTAA	7 cut(s)	633, 1176, 1482, 2004, 2309, 2577, 2661
TscAI	CASTG	2 cut(s)	1031, 2545
TseFI	GTSAC	2 cut(s)	1329, 2713
TseI	GCWGC	7 cut(s)	971, 1229, 1391, 1766, 1769, 1970, 2056
Tsp45I	GTSAC	2 cut(s)	1329, 2713
TspGWI	ACGGA	6 cut(s)	181, 262, 303, 573, 2544, 2732
TspRI	CASTG	2 cut(s)	1031, 2545
Vha464I	CTTAAG	1 cut(s)	1175
VpaK11BI	GGWCC	1 cut(s)	1723
XagI	CCTNNNNNAGG	1 cut(s)	1400
XapI	RAATTY	9 cut(s)	122, 179, 227, 414, 791, 1435, 1597, 1640, 2103
XceI	RCATGY	2 cut(s)	613, 1358
XcmI	CCANNNNNNNNNTGG	2 cut(s)	871, 2368
XmiI	GTMKAC	1 cut(s)	680
XmnI	GAANNNNTTC	2 cut(s)	437, 2280
XspI	CTAG	4 cut(s)	593, 1307, 2157, 2740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.