Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0000648

Physical Location & Seq

Genomic Coordinates Reverse (-)

70851259 .. 70864588

Gene Structure 13330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG513300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGGCGGAGCAGTTCAAAGGCCAACCCCGCCTTCCCAAATTCGCCGCCCCCAAACGCTACGACGTTAGCCTCAAACCGGACCTCGCCGCCTGCAAATTCGCCGGTTCCGTCGCCATCGACCTCGATATCGTCTCCGATACCAACTTCATTGTCCTCAATGCCGCCGAGCTCGCCGTCGACACCGCAACCGTCTCTTTCACTCACAAAAACTCTTCAGAGGTTTTCAAGCCTTTGAAAGCTGAGACATTCGAAGAAGATGGCATTTTGGTTTTGGAGTTTGGAGAGACACTTCCGACTGGGCCGGGAGTTTTGGCCATTGGGTTTGAAGGAATTTTGAATGACAAGATGAAGGGGTTCTATAGAAGTACATATGAGCATAATGGGGAGAAGAAAAACATGGCTGTTACACAGTTTGAACCGGTTGATGCCAGGCGCTGCTTTCCGTGTTGGGATGAACCTGCTTGCAAGGCTACATTCAAAATTACATTGGAAGATGTACCATCTGAATTGGTAGCTCTTTCCAACATGCCGGTTTCACAAGAAAAAGTGAATGGACATTGGAAGACAGTTTCATATCAGGAGACACCAATTATGTCTACATATTTGGTGGCCATTGTTGTTGGATTGTTCCATTACGTTGAAGATCACACTTCTGATGGGGTCAAAGTGAGGGTCTATTGTCAAGTTGGTAAGGAAAACCAAGGGAAATTTGCTTTGCAGGTTGCTGTCAAGACACTTGAATTGTACAAAAAATACTTTGGTGTGCCTTACCCTTTGCCAAAATTGGATATGGTCGCAATCCCTGATTTTTCGGCTGGGGCCATGGAGAATTATGGTTTAGTTACATACCGTGAGACAGCTTTGCTCTTTGATGATCAGCATTCTTCAGCTTCCATCAAGCAAAATGTTACAATAACCGTAGCACATGAATTGGCACACCAGTGGTTTGGCAATCTTGTAACCATGGAATGGTGGACACATTTATGGCTCAATGAGGGATTTGCAACATGGGTGAGCTATTTAGCTACTGAGAGCTTGTTTCCTGAGTGGAAAATATGGACTCAGTTTCTTGATGAATCTACTGAGGGTCTTAAGCTGGATGGTCTTGAAGAATCCCACCCCATCGAGGTGGAGATAAATCATGCTACTGAGGTTGATGAAATATTTGATGCAATAAGTTATACAAAAGGTGCTTCTGTTATTAGGATGCTACAAAATTATCTTGGTGCTGAACCCTTTCAGAGGTCACTGGCTGCATATGTAAAGAAACATGCTTACTCAAATGCAAAGACAGAAGACTTATGGGCTGCCCTTGAAGAGGGATCTGGTGAACCTGTGAACAAGCTGATGAATTCGTGGACGCAGCAGAAAGGCTACCCAGTTGTTTCTGTCGAAGTCAAAGATCAAAAATTGAAGTTTGATCAGACACAATTCTTGTCGAGTGGTTCCCAAGGCGAAGGAGAATGGATTGTGCCAATAACATTATGCTGTGGCTCGTATGATTTACGCAAGAGCTTCCTACTCAAAACTAAGTCTGAAACTCTTGACATAAAGGAATTTCTAGGTTGTTCAATAGCAGAGACTGAAAGTGGAAGCATATGCAATAAAAACAATACAGTATGCAGTTGGATAAAAATCAATGTAGATCAAGCTGGTTTTTACAGGGTGAAATATGATGAGAAACTTGCAGCTACACTAAGAAATGCTATACAGAACAAACACTTGTCTGCAACGGACAGATTTGGCATTTTGGATGATTCATATGCCCTTTCTATGGCTCGTCAGCTGTCTTTTGCTTCGTTGCTTACCTTGTTGGCTGCTTACAAAGAGGAACTTGACTATACAGTCCTGTCAAATTTGATTTCTATTAGTCAAAAGCTCGCAAGAATTGCAGCTGATGCAATACCCGAGCTACTTGATCTCATCAATCAGTTCTTTATTGGCCTTCTTCAGTACTCTGCAGAGAAGCTTGGTTGGCAGCCGAAACCCGGTGAGGGCCATTTAGATGCAATGTTGAGGGAAGAAGTTTTGACCACGCTTGCTTTATTTGGACATGACCTAACAATTAATGAAGCAAGCAGGCGTTTTCATGCCTACTTAGATGACAGAAATACGCCTCTCCTTCCTCCTGACATTAGAAAGGCAGCATATGTGGCTGTAATGCAAACAGTAACCCAATCAAACAGAGCAGGCTATGACTCTCTTCTTAGAGTTTATAGAGAGAGTGATCAAAGTGAGGAGAAAAACCGCATTTTAGGTTCATTAGCATCCTGTCCAGATCCAGACATTATACTGGAAGTTCTCAACTTTTTGTTGACTTCAGAGGTCCGCAACCAGGATGCTATTTTTGGACTTGCTGTTTGTATTAAAGGACGGGAAACAGCTTGGACTTGGCTGAAGGATAACTGGGAGAAAATCTCAAAGACCTGGGGTTCTGGATTTCTAATTACTCGCTTTGTCAGTGCAATTGTTTCACCGCATGCTTCATTTGAGAAGGTCGAGGAAGTAGAGGAGTTCTTCAAAGCCCATCCTAACCCTGCCATAACTAGAACCTTGAAGCAGAGCATGGAGCGAGTACAGATTAACGCCAAGTGGGTTCAGAGCGTTCAGGGTGAGAAAGATCTTCCCAAGGTTTTGAAGGAGTTGGCTTCCAGGAAATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006518 GO:0006807 GO:0006810 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009628 GO:0009914 GO:0009926 GO:0009987 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0031090 GO:0031406 GO:0033218 GO:0033293 GO:0033554 GO:0034641 GO:0036094 GO:0036293 GO:0036294 GO:0042221 GO:0042277 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043177 GO:0043226 GO:0043227 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060918 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ERAP1_C Peptidase_M1

Protein Analysis

887

Amino Acids

99.79

Weight (kDa)

5.34

Isoelectric Point (pI)

30.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	17 - 202	4.1e-51	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	237 - 453	2.7e-88	Peptidase family M1 domain
ERAP1_C	PF11838	543 - 860	5.1e-84	ERAP1-like C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000648)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33090
fragaria_vesca	FvH4_2g31431 FvH4_2g31440 FvH4_6g34250 FvH4_6g34262 FvH4_6g34263
malus_domestica	MD08G1066400.v1.1 MD08G1066500.v1.1 MD08G1067400.v1.1 MD09G1070300.v1.1 MD09G1187200.v1.1 MD10G1090900.v1.1 MD15G1054400.v1.1 MD17G1171400.v1.1
prunus_persica	Prupe.1G407900_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G408000_v2.0.a1 Prupe.1G457300_v2.0.a1 Prupe.3G044500_v2.0.a1 Prupe.3G044500_v2.0.a1
pyrus_communis	pycom08g05520 pycom08g05550 pycom08g05560 pycom08g05590 pycom09g10370 pycom09g10380 pycom10g07440 pycom10g07460 pycom15g05130 pycom15g05150 pycom17g16900
rosa_chinensis	RchiOBHm_Chr2g0144571 RchiOBHm_Chr2g0144611 RchiOBHm_Chr4g0390591 RchiOBHm_Chr6g0311371 RchiOBHm_Chr6g0311381
rosa_laevigata	RLG00000009010 RLG00000009289 RLG00000010411 RLG00000010412 RLG00000020090 RLG00000020092 RLG00000036974
rosa_multiflora	Rmu_co8212940.1_g000001 Rmu_sc0005789.1_g000001 Rmu_sc0008536.1_g000010 Rmu_sc0008536.1_g000011 Rmu_sc0009300.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056400 Rroxscaffold_2G00084720 Rroxscaffold_2G00101200 Rroxscaffold_7G00157670 Rroxscaffold_7G00157680
rosa_rugosa	Rorug02G0387900 Rorug02G0388000 Rorug02G0388100 Rorug06G0388300 Rorug06G0388400
rosa_samantha	Rh2AG440000 Rh2AG440100 Rh2BG450000 Rh2BG450300 Rh2BG450500 Rh2CG427000 Rh2CG427200 Rh2DG459900 Rh2DG460300 Rh2DG460500 Rh6AG502600 Rh6AG502700 Rh6BG513300 Rh6CG519100 Rh6CG519200
rosa_wichuraiana	Rw2G036000 Rw6G043770 Rw6G043780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1844
Acc36I	ACCTGC	2 cut(s)	466, 709
AccB7I	CCANNNNNTGG	1 cut(s)	969
AccI	GTMKAC	2 cut(s)	177, 596
AciI	CCGC	9 cut(s)	5, 28, 45, 87, 162, 183, 2248, 2329, 2477
AclWI	GGATC	2 cut(s)	1330, 2273
AcoI	YGGCCR	2 cut(s)	312, 609
AcsI	RAATTY	7 cut(s)	38, 95, 330, 707, 1351, 1556, 1855
AcuI	CTGAAG	5 cut(s)	198, 870, 1934, 2304, 2417
AfaI	GTAC	5 cut(s)	367, 498, 746, 1955, 2577
AfiI	CCNNNNNNNGG	9 cut(s)	76, 969, 1126, 1242, 1318, 1774, 1988, 1994, 2335
AflII	CTTAAG	1 cut(s)	1091
AgeI	ACCGGT	1 cut(s)	418
AjnI	CCWGG	4 cut(s)	428, 2334, 2426, 2651
AjuI	GAANNNNNNNTTGG	2 cut(s)	15, 47
AleI	CACNNNNGTG	1 cut(s)	940
Alw21I	GWGCWC	1 cut(s)	171
Alw26I	GTCTC	7 cut(s)	136, 196, 236, 278, 575, 848, 1574
AlwI	GGATC	2 cut(s)	1330, 2273
AlwNI	CAGNNNCTG	2 cut(s)	435, 1583
Ama87I	CYCGRG	1 cut(s)	1907
AoxI	GGCC	7 cut(s)	19, 299, 312, 609, 819, 1942, 1996
ApeKI	GCWGC	9 cut(s)	435, 1253, 1307, 1363, 1688, 1817, 1892, 1978, 2144
ApoI	RAATTY	7 cut(s)	38, 95, 330, 707, 1351, 1556, 1855
ArsI	GACNNNNNNTTYG	4 cut(s)	657, 689, 2343, 2375
AseI	ATTAAT	1 cut(s)	2067
AsiGI	ACCGGT	1 cut(s)	418
Asp700I	GAANNNNTTC	2 cut(s)	353, 1236
AspLEI	GCGC	1 cut(s)	435
AspS9I	GGNCC	5 cut(s)	79, 299, 819, 1996, 2326
AsuC2I	CCSGG	2 cut(s)	303, 1989
AsuHPI	GGTGA	6 cut(s)	1024, 1340, 1678, 2003, 2466, 2624
AsuII	TTCGAA	1 cut(s)	249
AvaI	CYCGRG	1 cut(s)	1907
AvaII	GGWCC	2 cut(s)	79, 2326
BalI	TGGCCA	2 cut(s)	314, 611
BanII	GRGCYC	1 cut(s)	171
BbsI	GAAGAC	2 cut(s)	569, 1302
Bbv12I	GWGCWC	1 cut(s)	171
BbvI	GCAGC	9 cut(s)	422, 1240, 1294, 1375, 1700, 1804, 1904, 1990, 2156
BccI	CCATC	8 cut(s)	122, 251, 508, 650, 902, 1094, 1130, 2535
BceAI	ACGGC	1 cut(s)	158
BciT130I	CCWGG	4 cut(s)	430, 2336, 2428, 2653
BclI	TGATCA	3 cut(s)	874, 1420, 2227
BcnI	CCSGG	2 cut(s)	303, 1989
BcoDI	GTCTC	7 cut(s)	136, 196, 236, 278, 575, 848, 1574
BfaI	CTAG	3 cut(s)	1562, 2547, 2662
BfmI	CTRYAG	2 cut(s)	358, 1959
BfoI	RGCGCY	1 cut(s)	436
BfrI	CTTAAG	1 cut(s)	1091
BfuAI	ACCTGC	2 cut(s)	466, 709
BglII	AGATCT	1 cut(s)	2620
BmcAI	AGTACT	1 cut(s)	1955
Bme1390I	CCNGG	6 cut(s)	303, 430, 1989, 2336, 2428, 2653
Bme18I	GGWCC	2 cut(s)	79, 2326
BmeT110I	CYCGRG	1 cut(s)	1907
BmgT120I	GGNCC	5 cut(s)	79, 299, 819, 1996, 2326
BmiI	GGNNCC	3 cut(s)	106, 820, 1447
BmrFI	CCNGG	6 cut(s)	303, 430, 1989, 2336, 2428, 2653
BmrI	ACTGGG	3 cut(s)	306, 1373, 2416
BmsI	GCATC	7 cut(s)	415, 1159, 1197, 1888, 1996, 2276, 2329
BmuI	ACTGGG	3 cut(s)	306, 1373, 2416
BpiI	GAAGAC	2 cut(s)	569, 1302
BplI	GAGNNNNNCTC	2 cut(s)	2402, 2434
Bpu14I	TTCGAA	1 cut(s)	249
BpuMI	CCSGG	2 cut(s)	303, 1989
BsaBI	GATNNNNATC	1 cut(s)	1634
BsaJI	CCNNGG	6 cut(s)	700, 822, 963, 1450, 2427, 2628
BsaWI	WCCGGW	2 cut(s)	76, 418
Bsc4I	CCNNNNNNNGG	9 cut(s)	76, 969, 1126, 1242, 1318, 1774, 1988, 1994, 2335
Bse118I	RCCGGY	3 cut(s)	101, 418, 529
Bse1I	ACTGG	6 cut(s)	301, 940, 1254, 1379, 2298, 2411
Bse3DI	GCAATG	1 cut(s)	2016
Bse8I	GATNNNNATC	1 cut(s)	1634
BseBI	CCWGG	4 cut(s)	430, 2336, 2428, 2653
BseDI	CCNNGG	6 cut(s)	700, 822, 963, 1450, 2427, 2628
BseGI	GGATG	7 cut(s)	457, 1105, 1212, 1759, 2267, 2344, 2527
BseJI	GATNNNNATC	1 cut(s)	1634
BseLI	CCNNNNNNNGG	9 cut(s)	76, 969, 1126, 1242, 1318, 1774, 1988, 1994, 2335
BseMI	GCAATG	1 cut(s)	2016
BseMII	CTCAG	6 cut(s)	231, 1020, 1035, 1074, 1076, 1140
BseNI	ACTGG	6 cut(s)	301, 940, 1254, 1379, 2298, 2411
BseRI	GAGGAG	2 cut(s)	2252, 2525
BseXI	GCAGC	9 cut(s)	422, 1240, 1294, 1375, 1700, 1804, 1904, 1990, 2156
BseYI	CCCAGC	1 cut(s)	815
BshFI	GGCC	7 cut(s)	21, 301, 314, 611, 821, 1944, 1998
BshTI	ACCGGT	1 cut(s)	418
BsiHKAI	GWGCWC	1 cut(s)	171
BsiHKCI	CYCGRG	1 cut(s)	1907
BsiSI	CCGG	6 cut(s)	77, 102, 302, 419, 530, 1989
BslI	CCNNNNNNNGG	9 cut(s)	76, 969, 1126, 1242, 1318, 1774, 1988, 1994, 2335
BsmAI	GTCTC	7 cut(s)	136, 196, 236, 278, 575, 848, 1574
BsmBI	CGTCTC	2 cut(s)	136, 196
BsmI	GAATGC	1 cut(s)	880
BsnI	GGCC	7 cut(s)	21, 301, 314, 611, 821, 1944, 1998
BsoBI	CYCGRG	1 cut(s)	1907
Bsp119I	TTCGAA	1 cut(s)	249
Bsp1286I	GDGCHC	1 cut(s)	171
Bsp1407I	TGTACA	1 cut(s)	744
Bsp19I	CCATGG	2 cut(s)	822, 963
BspACI	CCGC	9 cut(s)	5, 28, 45, 87, 162, 183, 2248, 2329, 2477
BspANI	GGCC	7 cut(s)	21, 301, 314, 611, 821, 1944, 1998
BspCNI	CTCAG	6 cut(s)	232, 1021, 1036, 1075, 1075, 1141
BspLI	GGNNCC	3 cut(s)	106, 820, 1447
BspMAI	CTGCAG	1 cut(s)	1963
BspMI	ACCTGC	2 cut(s)	466, 709
BspPI	GGATC	2 cut(s)	1330, 2273
BspT104I	TTCGAA	1 cut(s)	249
BspTI	CTTAAG	1 cut(s)	1091
BsrDI	GCAATG	1 cut(s)	2016
BsrFI	RCCGGY	3 cut(s)	101, 418, 529
BsrGI	TGTACA	1 cut(s)	744
BsrI	ACTGG	6 cut(s)	301, 940, 1254, 1379, 2298, 2411
BssAI	RCCGGY	3 cut(s)	101, 418, 529
BssECI	CCNNGG	6 cut(s)	700, 822, 963, 1450, 2427, 2628
BssT1I	CCWWGG	5 cut(s)	700, 822, 963, 1450, 2628
Bst2UI	CCWGG	4 cut(s)	430, 2336, 2428, 2653
Bst4CI	ACNGT	8 cut(s)	190, 411, 568, 851, 919, 1618, 1846, 2170
Bst6I	CTCTTC	3 cut(s)	217, 1311, 2208
BstAFI	CTTAAG	1 cut(s)	1091
BstAPI	GCANNNNNTGC	2 cut(s)	1889, 1898
BstAUI	TGTACA	1 cut(s)	744
BstBI	TTCGAA	1 cut(s)	249
BstC8I	GCNNGC	9 cut(s)	91, 171, 463, 1881, 2040, 2077, 2081, 2191, 2481
BstDSI	CCRYGG	2 cut(s)	822, 963
BstENI	CCTNNNNNAGG	2 cut(s)	1240, 1316
BstF5I	GGATG	7 cut(s)	457, 1105, 1212, 1759, 2267, 2344, 2527
BstH2I	RGCGCY	1 cut(s)	436
BstHHI	GCGC	1 cut(s)	435
BstMAI	GTCTC	7 cut(s)	136, 196, 236, 278, 575, 848, 1574
BstMWI	GCNNNNNNNGC	7 cut(s)	27, 170, 467, 1889, 1898, 1975, 2153
BstNI	CCWGG	4 cut(s)	430, 2336, 2428, 2653
BstNSI	RCATGY	3 cut(s)	529, 1274, 2483
BstSCI	CCNGG	6 cut(s)	301, 428, 1987, 2334, 2426, 2651
BstSFI	CTRYAG	2 cut(s)	358, 1959
BstV1I	GCAGC	9 cut(s)	422, 1240, 1294, 1375, 1700, 1804, 1904, 1990, 2156
BstV2I	GAAGAC	2 cut(s)	569, 1302
BstX2I	RGATCY	3 cut(s)	1322, 2278, 2620
BstXI	CCANNNNNNTGG	2 cut(s)	947, 1129
BstYI	RGATCY	3 cut(s)	1322, 2278, 2620
BsuRI	GGCC	7 cut(s)	21, 301, 314, 611, 821, 1944, 1998
BtgI	CCRYGG	2 cut(s)	822, 963
BtsCI	GGATG	7 cut(s)	457, 1105, 1212, 1759, 2267, 2344, 2527
BtsIMutI	CAGTG	3 cut(s)	947, 1247, 2467
BveI	ACCTGC	2 cut(s)	466, 709
Cac8I	GCNNGC	9 cut(s)	91, 171, 463, 1881, 2040, 2077, 2081, 2191, 2481
CaiI	CAGNNNCTG	2 cut(s)	435, 1583
CfoI	GCGC	1 cut(s)	435
Cfr10I	RCCGGY	3 cut(s)	101, 418, 529
Cfr13I	GGNCC	5 cut(s)	79, 299, 819, 1996, 2326
CseI	GACGC	1 cut(s)	1369
Csp6I	GTAC	5 cut(s)	366, 497, 745, 1954, 2576
CspAI	ACCGGT	1 cut(s)	418
CviQI	GTAC	5 cut(s)	366, 497, 745, 1954, 2576
DrdI	GACNNNNNNGTC	1 cut(s)	1844
DseDI	GACNNNNNNGTC	1 cut(s)	1844
EaeI	YGGCCR	2 cut(s)	312, 609
Eam1104I	CTCTTC	3 cut(s)	217, 1311, 2208
EarI	CTCTTC	3 cut(s)	217, 1311, 2208
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	169
Eco130I	CCWWGG	5 cut(s)	700, 822, 963, 1450, 2628
Eco24I	GRGCYC	1 cut(s)	171
Eco32I	GATATC	1 cut(s)	127
Eco47I	GGWCC	2 cut(s)	79, 2326
Eco53kI	GAGCTC	1 cut(s)	169
Eco57I	CTGAAG	5 cut(s)	198, 870, 1934, 2304, 2417
Eco88I	CYCGRG	1 cut(s)	1907
EcoICRI	GAGCTC	1 cut(s)	169
EcoNI	CCTNNNNNAGG	2 cut(s)	1240, 1316
EcoRI	GAATTC	1 cut(s)	1351
EcoRII	CCWGG	4 cut(s)	428, 2334, 2426, 2651
EcoRV	GATATC	1 cut(s)	127
EcoT14I	CCWWGG	5 cut(s)	700, 822, 963, 1450, 2628
EcoT38I	GRGCYC	1 cut(s)	171
ErhI	CCWWGG	5 cut(s)	700, 822, 963, 1450, 2628
Esp3I	CGTCTC	2 cut(s)	136, 196
FalI	AAGNNNNNCTT	2 cut(s)	1818, 1850
FauI	CCCGC	1 cut(s)	35
FauNDI	CATATG	5 cut(s)	370, 1258, 1598, 1762, 2149
FbaI	TGATCA	3 cut(s)	874, 1420, 2227
FblI	GTMKAC	2 cut(s)	177, 596
FokI	GGATG	7 cut(s)	464, 1112, 1219, 1766, 2254, 2351, 2514
FriOI	GRGCYC	1 cut(s)	171
FspBI	CTAG	3 cut(s)	1562, 2547, 2662
GlaI	GCGC	1 cut(s)	434
GsaI	CCCAGC	1 cut(s)	819
HaeII	RGCGCY	1 cut(s)	436
HaeIII	GGCC	7 cut(s)	21, 301, 314, 611, 821, 1944, 1998
HapII	CCGG	6 cut(s)	77, 102, 302, 419, 530, 1989
HgaI	GACGC	1 cut(s)	1369
HhaI	GCGC	1 cut(s)	435
Hin6I	GCGC	1 cut(s)	433
HinP1I	GCGC	1 cut(s)	433
HincII	GTYRAC	2 cut(s)	178, 2316
HindII	GTYRAC	2 cut(s)	178, 2316
HindIII	AAGCTT	1 cut(s)	1967
HinfI	GANTC	5 cut(s)	1060, 1076, 1112, 1757, 2198
HpaII	CCGG	6 cut(s)	77, 102, 302, 419, 530, 1989
HphI	GGTGA	6 cut(s)	1024, 1340, 1678, 2003, 2466, 2624
Hpy166II	GTNNAC	7 cut(s)	178, 597, 975, 1331, 1339, 1359, 2316
Hpy8I	GTNNAC	7 cut(s)	178, 597, 975, 1331, 1339, 1359, 2316
Hpy99I	CGWCG	3 cut(s)	65, 113, 179
HpyAV	CCTTC	9 cut(s)	41, 320, 343, 1451, 1955, 2132, 2392, 2488, 2632
HpyCH4III	ACNGT	8 cut(s)	190, 411, 568, 851, 919, 1618, 1846, 2170
HpyCH4IV	ACGT	2 cut(s)	63, 636
HpyF10VI	GCNNNNNNNGC	7 cut(s)	27, 170, 467, 1889, 1898, 1975, 2153
HpySE526I	ACGT	2 cut(s)	63, 636
HspAI	GCGC	1 cut(s)	433
Ksp22I	TGATCA	3 cut(s)	874, 1420, 2227
LmnI	GCTCC	2 cut(s)	7, 2569
Lsp1109I	GCAGC	9 cut(s)	422, 1240, 1294, 1375, 1700, 1804, 1904, 1990, 2156
LweI	GCATC	7 cut(s)	415, 1159, 1197, 1888, 1996, 2276, 2329
MaeI	CTAG	3 cut(s)	1562, 2547, 2662
MaeII	ACGT	2 cut(s)	63, 636
MaeIII	GTNAC	6 cut(s)	403, 841, 907, 958, 1245, 2170
MfeI	CAATTG	1 cut(s)	2466
MflI	RGATCY	3 cut(s)	1322, 2278, 2620
MhlI	GDGCHC	1 cut(s)	171
MlsI	TGGCCA	2 cut(s)	314, 611
MluNI	TGGCCA	2 cut(s)	314, 611
MlyI	GAGTC	2 cut(s)	1054, 2192
MmeI	TCCRAC	4 cut(s)	317, 546, 601, 1607
Mox20I	TGGCCA	2 cut(s)	314, 611
MroXI	GAANNNNTTC	2 cut(s)	353, 1236
MscI	TGGCCA	2 cut(s)	314, 611
MseI	TTAA	4 cut(s)	1092, 2067, 2367, 2583
MslI	CAYNNNNRTG	4 cut(s)	940, 982, 1127, 2004
Msp20I	TGGCCA	2 cut(s)	314, 611
MspA1I	CMGCKG	2 cut(s)	1786, 1895
MspCI	CTTAAG	1 cut(s)	1091
MspI	CCGG	6 cut(s)	77, 102, 302, 419, 530, 1989
MspR9I	CCNGG	6 cut(s)	303, 430, 1989, 2336, 2428, 2653
MunI	CAATTG	1 cut(s)	2466
Mva1269I	GAATGC	1 cut(s)	880
MvaI	CCWGG	4 cut(s)	430, 2336, 2428, 2653
MwoI	GCNNNNNNNGC	7 cut(s)	27, 170, 467, 1889, 1898, 1975, 2153
NciI	CCSGG	2 cut(s)	303, 1989
NcoI	CCATGG	2 cut(s)	822, 963
NdeI	CATATG	5 cut(s)	370, 1258, 1598, 1762, 2149
NlaIV	GGNNCC	3 cut(s)	106, 820, 1447
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	1 cut(s)	1245
NspI	RCATGY	3 cut(s)	529, 1274, 2483
NspV	TTCGAA	1 cut(s)	249
OliI	CACNNNNGTG	1 cut(s)	940
PaeI	GCATGC	1 cut(s)	2483
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	1 cut(s)	880
PdmI	GAANNNNTTC	2 cut(s)	353, 1236
PfeI	GAWTC	3 cut(s)	1076, 1112, 1757
PflMI	CCANNNNNTGG	1 cut(s)	969
PfoI	TCCNGGA	1 cut(s)	2651
PinAI	ACCGGT	1 cut(s)	418
PleI	GAGTC	2 cut(s)	1054, 2192
PpsI	GAGTC	2 cut(s)	1054, 2192
PshBI	ATTAAT	1 cut(s)	2067
Psp124BI	GAGCTC	1 cut(s)	171
Psp6I	CCWGG	4 cut(s)	428, 2334, 2426, 2651
PspFI	CCCAGC	1 cut(s)	815
PspGI	CCWGG	4 cut(s)	428, 2334, 2426, 2651
PspN4I	GGNNCC	3 cut(s)	106, 820, 1447
PspPI	GGNCC	5 cut(s)	79, 299, 819, 1996, 2326
PstI	CTGCAG	1 cut(s)	1963
PstNI	CAGNNNCTG	2 cut(s)	435, 1583
PsuI	RGATCY	3 cut(s)	1322, 2278, 2620
PvuII	CAGCTG	2 cut(s)	1786, 1895
RsaI	GTAC	5 cut(s)	367, 498, 746, 1955, 2577
RsaNI	GTAC	5 cut(s)	366, 497, 745, 1954, 2576
RseI	CAYNNNNRTG	4 cut(s)	940, 982, 1127, 2004
SacI	GAGCTC	1 cut(s)	171
SalI	GTCGAC	1 cut(s)	176
SaqAI	TTAA	4 cut(s)	1092, 2067, 2367, 2583
Sau96I	GGNCC	5 cut(s)	79, 299, 819, 1996, 2326
ScaI	AGTACT	1 cut(s)	1955
SchI	GAGTC	2 cut(s)	1054, 2192
ScrFI	CCNGG	6 cut(s)	303, 430, 1989, 2336, 2428, 2653
SduI	GDGCHC	1 cut(s)	171
SfaNI	GCATC	7 cut(s)	415, 1159, 1197, 1888, 1996, 2276, 2329
SfcI	CTRYAG	2 cut(s)	358, 1959
SfuI	TTCGAA	1 cut(s)	249
SinI	GGWCC	2 cut(s)	79, 2326
SmiMI	CAYNNNNRTG	4 cut(s)	940, 982, 1127, 2004
SmlI	CTYRAG	1 cut(s)	1091
SmoI	CTYRAG	1 cut(s)	1091
SphI	GCATGC	1 cut(s)	2483
SsiI	CCGC	9 cut(s)	5, 28, 45, 87, 162, 183, 2248, 2329, 2477
SspI	AATATT	1 cut(s)	1164
SspMI	CTAG	3 cut(s)	1562, 2547, 2662
SstI	GAGCTC	1 cut(s)	171
StyD4I	CCNGG	6 cut(s)	301, 428, 1987, 2334, 2426, 2651
StyI	CCWWGG	5 cut(s)	700, 822, 963, 1450, 2628
TaaI	ACNGT	8 cut(s)	190, 411, 568, 851, 919, 1618, 1846, 2170
TaiI	ACGT	2 cut(s)	66, 639
TaqI	TCGA	8 cut(s)	117, 123, 177, 249, 1125, 1392, 1439, 2499
TatI	WGTACW	4 cut(s)	365, 744, 1953, 2575
TauI	GCSGC	3 cut(s)	47, 89, 164
TfiI	GAWTC	3 cut(s)	1076, 1112, 1757
Tru1I	TTAA	4 cut(s)	1092, 2067, 2367, 2583
Tru9I	TTAA	4 cut(s)	1092, 2067, 2367, 2583
TscAI	CASTG	3 cut(s)	947, 1254, 2467
TseFI	GTSAC	1 cut(s)	1245
TseI	GCWGC	9 cut(s)	435, 1253, 1307, 1363, 1688, 1817, 1892, 1978, 2144
Tsp45I	GTSAC	1 cut(s)	1245
TspGWI	ACGGA	3 cut(s)	97, 432, 1748
TspRI	CASTG	3 cut(s)	947, 1254, 2467
Van91I	CCANNNNNTGG	1 cut(s)	969
Vha464I	CTTAAG	1 cut(s)	1091
VpaK11BI	GGWCC	2 cut(s)	79, 2326
VspI	ATTAAT	1 cut(s)	2067
XagI	CCTNNNNNAGG	2 cut(s)	1240, 1316
XapI	RAATTY	7 cut(s)	38, 95, 330, 707, 1351, 1556, 1855
XceI	RCATGY	3 cut(s)	529, 1274, 2483
XmiI	GTMKAC	2 cut(s)	177, 596
XmnI	GAANNNNTTC	2 cut(s)	353, 1236
XspI	CTAG	3 cut(s)	1562, 2547, 2662
ZrmI	AGTACT	1 cut(s)	1955

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.