Aminopeptidase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 775 |
| Acc36I | ACCTGC | 1 cut(s) | 727 |
| AccII | CGCG | 1 cut(s) | 602 |
| AciI | CCGC | 4 cut(s) | 487, 694, 758, 1342 |
| AclWI | GGATC | 2 cut(s) | 115, 1124 |
| AcoI | YGGCCR | 2 cut(s) | 475, 1281 |
| AcsI | RAATTY | 2 cut(s) | 304, 1064 |
| AdeI | CACNNNGTG | 1 cut(s) | 554 |
| AfaI | GTAC | 6 cut(s) | 123, 169, 756, 921, 1028, 1106 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 104, 1242 |
| AflIII | ACRYGT | 1 cut(s) | 164 |
| AjnI | CCWGG | 1 cut(s) | 1212 |
| AleI | CACNNNNGTG | 2 cut(s) | 694, 1198 |
| AluBI | AGCT | 5 cut(s) | 343, 468, 766, 773, 805 |
| AluI | AGCT | 5 cut(s) | 343, 468, 766, 773, 805 |
| Alw26I | GTCTC | 2 cut(s) | 401, 846 |
| AlwI | GGATC | 2 cut(s) | 115, 1124 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1061 |
| Ama87I | CYCGRG | 1 cut(s) | 1299 |
| AoxI | GGCC | 5 cut(s) | 286, 475, 729, 1281, 1343 |
| ApoI | RAATTY | 2 cut(s) | 304, 1064 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 614 |
| AspLEI | GCGC | 3 cut(s) | 365, 604, 1183 |
| AspS9I | GGNCC | 2 cut(s) | 286, 1233 |
| AsuHPI | GGTGA | 5 cut(s) | 182, 454, 524, 572, 1228 |
| AsuII | TTCGAA | 1 cut(s) | 675 |
| AvaI | CYCGRG | 1 cut(s) | 1299 |
| AvaII | GGWCC | 1 cut(s) | 1233 |
| BalI | TGGCCA | 2 cut(s) | 477, 1283 |
| BanII | GRGCYC | 1 cut(s) | 1319 |
| BbsI | GAAGAC | 1 cut(s) | 906 |
| BccI | CCATC | 3 cut(s) | 247, 632, 908 |
| BceAI | ACGGC | 2 cut(s) | 707, 1009 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1034, 1068 |
| BciT130I | CCWGG | 1 cut(s) | 1214 |
| BclI | TGATCA | 2 cut(s) | 541, 1098 |
| BcoDI | GTCTC | 2 cut(s) | 401, 846 |
| BfaI | CTAG | 2 cut(s) | 44, 1347 |
| BfmI | CTRYAG | 1 cut(s) | 588 |
| BfuAI | ACCTGC | 1 cut(s) | 727 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 728 |
| BglII | AGATCT | 1 cut(s) | 46 |
| BisI | GCNGC | 1 cut(s) | 1343 |
| BlsI | GCNGC | 1 cut(s) | 1344 |
| Bme1390I | CCNGG | 1 cut(s) | 1214 |
| Bme18I | GGWCC | 1 cut(s) | 1233 |
| BmeT110I | CYCGRG | 1 cut(s) | 1299 |
| BmgT120I | GGNCC | 2 cut(s) | 286, 1233 |
| BmrFI | CCNGG | 1 cut(s) | 1214 |
| BmsI | GCATC | 2 cut(s) | 676, 799 |
| BpiI | GAAGAC | 1 cut(s) | 906 |
| Bpu14I | TTCGAA | 1 cut(s) | 675 |
| BpuEI | CTTGAG | 2 cut(s) | 323, 710 |
| BsaAI | YACGTR | 1 cut(s) | 1104 |
| BsaBI | GATNNNNATC | 1 cut(s) | 333 |
| BsaI | GGTCTC | 1 cut(s) | 401 |
| BsaJI | CCNNGG | 1 cut(s) | 220 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 398, 428 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 104, 1242 |
| Bse1I | ACTGG | 2 cut(s) | 927, 1343 |
| Bse3DI | GCAATG | 1 cut(s) | 1086 |
| Bse8I | GATNNNNATC | 1 cut(s) | 333 |
| BseBI | CCWGG | 1 cut(s) | 1214 |
| BseDI | CCNNGG | 1 cut(s) | 220 |
| BseGI | GGATG | 3 cut(s) | 643, 691, 908 |
| BseJI | GATNNNNATC | 1 cut(s) | 333 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 104, 1242 |
| BseMI | GCAATG | 1 cut(s) | 1086 |
| BseMII | CTCAG | 1 cut(s) | 240 |
| BseNI | ACTGG | 2 cut(s) | 927, 1343 |
| BseRI | GAGGAG | 4 cut(s) | 205, 419, 657, 851 |
| Bsh1236I | CGCG | 1 cut(s) | 602 |
| BshFI | GGCC | 5 cut(s) | 288, 477, 731, 1283, 1345 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1299 |
| BsiSI | CCGG | 2 cut(s) | 372, 761 |
| BslFI | GGGAC | 1 cut(s) | 725 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 104, 1242 |
| BsmAI | GTCTC | 2 cut(s) | 401, 846 |
| BsmFI | GGGAC | 1 cut(s) | 725 |
| BsmI | GAATGC | 2 cut(s) | 270, 1138 |
| BsnI | GGCC | 5 cut(s) | 288, 477, 731, 1283, 1345 |
| Bso31I | GGTCTC | 1 cut(s) | 401 |
| BsoBI | CYCGRG | 1 cut(s) | 1299 |
| Bsp119I | TTCGAA | 1 cut(s) | 675 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1319 |
| BspACI | CCGC | 4 cut(s) | 487, 694, 758, 1342 |
| BspANI | GGCC | 5 cut(s) | 288, 477, 731, 1283, 1345 |
| BspCNI | CTCAG | 1 cut(s) | 241 |
| BspFNI | CGCG | 1 cut(s) | 602 |
| BspHI | TCATGA | 1 cut(s) | 448 |
| BspMAI | CTGCAG | 1 cut(s) | 592 |
| BspMI | ACCTGC | 1 cut(s) | 727 |
| BspPI | GGATC | 2 cut(s) | 115, 1124 |
| BspT104I | TTCGAA | 1 cut(s) | 675 |
| BspTNI | GGTCTC | 1 cut(s) | 401 |
| BsrDI | GCAATG | 1 cut(s) | 1086 |
| BsrI | ACTGG | 2 cut(s) | 927, 1343 |
| BssECI | CCNNGG | 1 cut(s) | 220 |
| BssT1I | CCWWGG | 1 cut(s) | 220 |
| Bst2UI | CCWGG | 1 cut(s) | 1214 |
| Bst4CI | ACNGT | 3 cut(s) | 512, 826, 1109 |
| Bst6I | CTCTTC | 1 cut(s) | 944 |
| BstBAI | YACGTR | 1 cut(s) | 1104 |
| BstBI | TTCGAA | 1 cut(s) | 675 |
| BstC8I | GCNNGC | 1 cut(s) | 361 |
| BstDEI | CTNAG | 1 cut(s) | 249 |
| BstF5I | GGATG | 3 cut(s) | 643, 691, 908 |
| BstFNI | CGCG | 1 cut(s) | 602 |
| BstHHI | GCGC | 3 cut(s) | 365, 604, 1183 |
| BstMAI | GTCTC | 2 cut(s) | 401, 846 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 474, 728 |
| BstNI | CCWGG | 1 cut(s) | 1214 |
| BstNSI | RCATGY | 1 cut(s) | 363 |
| BstSCI | CCNGG | 1 cut(s) | 1212 |
| BstSFI | CTRYAG | 1 cut(s) | 588 |
| BstUI | CGCG | 1 cut(s) | 602 |
| BstV2I | GAAGAC | 1 cut(s) | 906 |
| BstX2I | RGATCY | 1 cut(s) | 46 |
| BstYI | RGATCY | 1 cut(s) | 46 |
| BsuRI | GGCC | 5 cut(s) | 288, 477, 731, 1283, 1345 |
| BtsCI | GGATG | 3 cut(s) | 643, 691, 908 |
| BtsIMutI | CAGTG | 2 cut(s) | 246, 1205 |
| BveI | ACCTGC | 1 cut(s) | 727 |
| Cac8I | GCNNGC | 1 cut(s) | 361 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1061 |
| CciI | TCATGA | 1 cut(s) | 448 |
| CfoI | GCGC | 3 cut(s) | 365, 604, 1183 |
| Cfr13I | GGNCC | 2 cut(s) | 286, 1233 |
| CseI | GACGC | 1 cut(s) | 1000 |
| CsiI | ACCWGGT | 1 cut(s) | 1212 |
| Csp6I | GTAC | 6 cut(s) | 122, 168, 755, 920, 1027, 1105 |
| CviAII | CATG | 5 cut(s) | 360, 449, 473, 850, 1266 |
| CviQI | GTAC | 6 cut(s) | 122, 168, 755, 920, 1027, 1105 |
| DdeI | CTNAG | 1 cut(s) | 249 |
| DraIII | CACNNNGTG | 1 cut(s) | 554 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 775 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 775 |
| EaeI | YGGCCR | 2 cut(s) | 475, 1281 |
| Eam1104I | CTCTTC | 1 cut(s) | 944 |
| EarI | CTCTTC | 1 cut(s) | 944 |
| Eco130I | CCWWGG | 1 cut(s) | 220 |
| Eco24I | GRGCYC | 1 cut(s) | 1319 |
| Eco31I | GGTCTC | 1 cut(s) | 401 |
| Eco47I | GGWCC | 1 cut(s) | 1233 |
| Eco88I | CYCGRG | 1 cut(s) | 1299 |
| EcoRII | CCWGG | 1 cut(s) | 1212 |
| EcoT14I | CCWWGG | 1 cut(s) | 220 |
| EcoT38I | GRGCYC | 1 cut(s) | 1319 |
| ErhI | CCWWGG | 1 cut(s) | 220 |
| FaeI | CATG | 5 cut(s) | 363, 452, 476, 853, 1269 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 719, 751 |
| FaqI | GGGAC | 1 cut(s) | 725 |
| FatI | CATG | 5 cut(s) | 359, 448, 472, 849, 1265 |
| FbaI | TGATCA | 2 cut(s) | 541, 1098 |
| Fnu4HI | GCNGC | 1 cut(s) | 1343 |
| FokI | GGATG | 3 cut(s) | 650, 698, 895 |
| FriOI | GRGCYC | 1 cut(s) | 1319 |
| Fsp4HI | GCNGC | 1 cut(s) | 1343 |
| FspBI | CTAG | 2 cut(s) | 44, 1347 |
| GlaI | GCGC | 3 cut(s) | 364, 603, 1182 |
| GluI | GCNGC | 1 cut(s) | 1343 |
| HaeIII | GGCC | 5 cut(s) | 288, 477, 731, 1283, 1345 |
| HapII | CCGG | 2 cut(s) | 372, 761 |
| HgaI | GACGC | 1 cut(s) | 1000 |
| HhaI | GCGC | 3 cut(s) | 365, 604, 1183 |
| Hin1II | CATG | 5 cut(s) | 363, 452, 476, 853, 1269 |
| Hin6I | GCGC | 3 cut(s) | 363, 602, 1181 |
| HinP1I | GCGC | 3 cut(s) | 363, 602, 1181 |
| HincII | GTYRAC | 1 cut(s) | 855 |
| HindII | GTYRAC | 1 cut(s) | 855 |
| HindIII | AAGCTT | 2 cut(s) | 341, 803 |
| HinfI | GANTC | 6 cut(s) | 94, 142, 279, 385, 521, 839 |
| HpaII | CCGG | 2 cut(s) | 372, 761 |
| HphI | GGTGA | 5 cut(s) | 182, 454, 524, 572, 1228 |
| Hpy166II | GTNNAC | 4 cut(s) | 168, 227, 462, 855 |
| Hpy188I | TCNGA | 5 cut(s) | 93, 187, 318, 913, 1237 |
| Hpy188III | TCNNGA | 9 cut(s) | 44, 50, 146, 424, 434, 449, 739, 1061, 1299 |
| Hpy8I | GTNNAC | 4 cut(s) | 168, 227, 462, 855 |
| HpyAV | CCTTC | 3 cut(s) | 376, 557, 745 |
| HpyCH4III | ACNGT | 3 cut(s) | 512, 826, 1109 |
| HpyCH4IV | ACGT | 4 cut(s) | 164, 175, 390, 1103 |
| HpyCH4V | TGCA | 9 cut(s) | 156, 311, 590, 627, 689, 812, 932, 1055, 1138 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 474, 728 |
| HpyF3I | CTNAG | 1 cut(s) | 249 |
| HpySE526I | ACGT | 4 cut(s) | 164, 175, 390, 1103 |
| Hsp92II | CATG | 5 cut(s) | 363, 452, 476, 853, 1269 |
| HspAI | GCGC | 3 cut(s) | 363, 602, 1181 |
| Ksp22I | TGATCA | 2 cut(s) | 541, 1098 |
| LmnI | GCTCC | 2 cut(s) | 256, 763 |
| LweI | GCATC | 2 cut(s) | 676, 799 |
| MabI | ACCWGGT | 1 cut(s) | 1212 |
| MaeI | CTAG | 2 cut(s) | 44, 1347 |
| MaeII | ACGT | 4 cut(s) | 164, 175, 390, 1103 |
| MaeIII | GTNAC | 3 cut(s) | 664, 953, 1216 |
| MboII | GAAGA | 8 cut(s) | 65, 323, 410, 661, 664, 911, 961, 1317 |
| MfeI | CAATTG | 1 cut(s) | 1170 |
| MflI | RGATCY | 1 cut(s) | 46 |
| MhlI | GDGCHC | 1 cut(s) | 1319 |
| MlsI | TGGCCA | 2 cut(s) | 477, 1283 |
| MluCI | AATT | 9 cut(s) | 63, 304, 312, 671, 789, 1064, 1087, 1170, 1320 |
| MluNI | TGGCCA | 2 cut(s) | 477, 1283 |
| MlyI | GAGTC | 1 cut(s) | 848 |
| MmeI | TCCRAC | 1 cut(s) | 234 |
| MnlI | CCTC | 8 cut(s) | 98, 226, 397, 635, 734, 829, 1041, 1246 |
| Mox20I | TGGCCA | 2 cut(s) | 477, 1283 |
| MroXI | GAANNNNTTC | 1 cut(s) | 614 |
| MscI | TGGCCA | 2 cut(s) | 477, 1283 |
| MseI | TTAA | 5 cut(s) | 597, 819, 987, 1248, 1351 |
| MslI | CAYNNNNRTG | 4 cut(s) | 251, 632, 694, 1198 |
| Msp20I | TGGCCA | 2 cut(s) | 477, 1283 |
| MspI | CCGG | 2 cut(s) | 372, 761 |
| MspR9I | CCNGG | 1 cut(s) | 1214 |
| MunI | CAATTG | 1 cut(s) | 1170 |
| Mva1269I | GAATGC | 2 cut(s) | 270, 1138 |
| MvaI | CCWGG | 1 cut(s) | 1214 |
| MvnI | CGCG | 1 cut(s) | 602 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 474, 728 |
| NlaIII | CATG | 5 cut(s) | 363, 452, 476, 853, 1269 |
| NmuCI | GTSAC | 2 cut(s) | 953, 1216 |
| NspI | RCATGY | 1 cut(s) | 363 |
| NspV | TTCGAA | 1 cut(s) | 675 |
| OliI | CACNNNNGTG | 2 cut(s) | 694, 1198 |
| PaeI | GCATGC | 1 cut(s) | 363 |
| PagI | TCATGA | 1 cut(s) | 448 |
| PctI | GAATGC | 2 cut(s) | 270, 1138 |
| PdmI | GAANNNNTTC | 1 cut(s) | 614 |
| PfeI | GAWTC | 5 cut(s) | 94, 142, 279, 385, 521 |
| PkrI | GCNGC | 1 cut(s) | 1344 |
| PleI | GAGTC | 1 cut(s) | 847 |
| PpsI | GAGTC | 1 cut(s) | 847 |
| Ppu21I | YACGTR | 1 cut(s) | 1104 |
| Psp6I | CCWGG | 1 cut(s) | 1212 |
| PspGI | CCWGG | 1 cut(s) | 1212 |
| PspPI | GGNCC | 2 cut(s) | 286, 1233 |
| PstI | CTGCAG | 1 cut(s) | 592 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1061 |
| PsuI | RGATCY | 1 cut(s) | 46 |
| RsaI | GTAC | 6 cut(s) | 123, 169, 756, 921, 1028, 1106 |
| RsaNI | GTAC | 6 cut(s) | 122, 168, 755, 920, 1027, 1105 |
| RseI | CAYNNNNRTG | 4 cut(s) | 251, 632, 694, 1198 |
| SaqAI | TTAA | 5 cut(s) | 597, 819, 987, 1248, 1351 |
| SatI | GCNGC | 1 cut(s) | 1343 |
| Sau96I | GGNCC | 2 cut(s) | 286, 1233 |
| SchI | GAGTC | 1 cut(s) | 848 |
| ScrFI | CCNGG | 1 cut(s) | 1214 |
| SduI | GDGCHC | 1 cut(s) | 1319 |
| SexAI | ACCWGGT | 1 cut(s) | 1212 |
| SfaNI | GCATC | 2 cut(s) | 676, 799 |
| SfcI | CTRYAG | 1 cut(s) | 588 |
| SfuI | TTCGAA | 1 cut(s) | 675 |
| SinI | GGWCC | 1 cut(s) | 1233 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 251, 632, 694, 1198 |
| SmlI | CTYRAG | 2 cut(s) | 338, 725 |
| SmoI | CTYRAG | 2 cut(s) | 338, 725 |
| SphI | GCATGC | 1 cut(s) | 363 |
| Sse9I | AATT | 9 cut(s) | 63, 304, 312, 671, 789, 1064, 1087, 1170, 1320 |
| SsiI | CCGC | 4 cut(s) | 487, 694, 758, 1342 |
| SspMI | CTAG | 2 cut(s) | 44, 1347 |
| StyD4I | CCNGG | 1 cut(s) | 1212 |
| StyI | CCWWGG | 1 cut(s) | 220 |
| TaaI | ACNGT | 3 cut(s) | 512, 826, 1109 |
| TaiI | ACGT | 4 cut(s) | 167, 178, 393, 1106 |
| TaqI | TCGA | 5 cut(s) | 197, 675, 888, 996, 1044 |
| TasI | AATT | 9 cut(s) | 63, 304, 312, 671, 789, 1064, 1087, 1170, 1320 |
| TatI | WGTACW | 1 cut(s) | 167 |
| TauI | GCSGC | 1 cut(s) | 1345 |
| TfiI | GAWTC | 5 cut(s) | 94, 142, 279, 385, 521 |
| Tru1I | TTAA | 5 cut(s) | 597, 819, 987, 1248, 1351 |
| Tru9I | TTAA | 5 cut(s) | 597, 819, 987, 1248, 1351 |
| TscAI | CASTG | 2 cut(s) | 253, 1205 |
| TseFI | GTSAC | 2 cut(s) | 953, 1216 |
| Tsp45I | GTSAC | 2 cut(s) | 953, 1216 |
| TspDTI | ATGAA | 5 cut(s) | 323, 437, 465, 534, 1131 |
| TspRI | CASTG | 2 cut(s) | 253, 1205 |
| VpaK11BI | GGWCC | 1 cut(s) | 1233 |
| XapI | RAATTY | 2 cut(s) | 304, 1064 |
| XbaI | TCTAGA | 1 cut(s) | 43 |
| XceI | RCATGY | 1 cut(s) | 363 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1045 |
| XmnI | GAANNNNTTC | 1 cut(s) | 614 |
| XspI | CTAG | 2 cut(s) | 44, 1347 |