Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000542

Physical Location & Seq

Genomic Coordinates Forward (+)

25983968 .. 25988732

Gene Structure 4765 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1179600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGTTCTACTCGCACCAGCTTCTAGCTCGGAAAGCTCCTCTGGGTCAAATCTGGGCTGCTACAATGCACGCTAAAATCAACCGCAGGAAGCTCGATAAGCTCGATCTCATCAAGATCTGCGAAGAGATTTTGAATCCTTCGGTTCCCATGGCTCTTCGTCTCTCCGGGATTCTCATGGGTGGAGTCGTCATCGTCTACGAACGAAAAGTGAAGCTCCTATACGAGGATGCGACTCGTCTACTGCGGCTTGATAGTGTCGATGAACAATATGTTAACAACAACGCAGCAGGGGAGGAAGATCCAGCTCCGCAACTCTACCAAGGTTGTATCATTCTTCTATCTGATTTTGTCGTAATTTTAAGATTTGAGATCGAAGGGGATGAAGAGACACAGTTTAACTTCACGTCAGGAGAGCACACACACATTCCCATACCCCCTTCCCCTCCTCGCGATGAACATCAGGAAGGGCCTCCTGCAGCTCATACAAACCAAGATGATCAACATCTAGATCACCAGGCCAATCAGCCAGATGAAAGCAAGAAATTTAGACAGATGAAGCTGGTTCAAGAGAGACAGGGGACTAGAAAAAGGAAAACAAGAAAGCAAGCATCTGGAATGGATTTTGAACAAACAATCATTCCTGGTCATGTATACCAATCTTGGCTCCGAGATCCTTCAGATCTATCACGAGATGCAAGAAAACGAAAGCGCAAAGATGTAATGGCAACTATGAAGATGGCTAACCTCATGGACCTGCCACCTAAAGTACTAATTGAAGATTTATTTACAACTGGAAGTAAAAACGTATATTATCCACGTCCTCTCCTTGACATGTGGATGAAAAGTGTCCGAACTCCCCGTGAGTCTCCTGCCGGACGAACCACTTCACCCCTGCCCCCAGGACCATCGTCACCAATACCGCCTGAGAGACAAAACTCTAATGGTCCAGTGGAATATCCTTTTCAAGATTTTCGTAGTGGAGTTGGCTCCCAGTCTTCCATAGATAAGCAACGGCCAAGTTCTGGTGTGGCAAAAGGCAAGAATGATCCAACAAGTGTACTCATGGAAGGACTGACTGCTAATCTTAAGAACATTGGCTTAGAGGGAAATGATACCAATCCAATGGCCACGCCTGGAAATTCTGATGACGTAAGATCCATCCCGAGCTCAGCATCAGGGCAGGCAATTCCTTCAGAAGGCAATTCAGGACGATCCGGCAAGAAAAGGCCTCATTCTGCATCCGGGGGCAACATTAGACTTGAACCACTATTGGAAATGAGTCACCCCGATGTACATTTCGAGTTGTCGAGGTCACCTGAGCAAGGCCCGGCATTTGATCAAGAACTCTTGGTGGAAACAGGACCAACTCAAACTCAAAAACCGATCGTGGAACAACCGCTTGACAAAACAACTGATACTATCAGAAGGGTTCTGAAATCACATTTTGAGACGGAGGGAGCTCCTCAGGTCGAATCCCTGGACAACCTAACTGCTGGAAGGAACCGAAAAGAAGCAGCTATGCTCTTCTACAAAACTTGTGTTCTGGCTACACGGGATGTCATAAGAGTCGAGCAGAGGACCGCTTACGGGGAGATTCTCATATCGAGAGGGCCGAAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000793 GO:0000794 GO:0000798 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006323 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007127 GO:0007135 GO:0008150 GO:0008152 GO:0008278 GO:0008608 GO:0009987 GO:0010032 GO:0016043 GO:0022402 GO:0022414 GO:0030261 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0045144 GO:0046483 GO:0048285 GO:0050896 GO:0051177 GO:0051276 GO:0051316 GO:0051321 GO:0051455 GO:0051716 GO:0051754 GO:0061982 GO:0061983 GO:0070013 GO:0070192 GO:0070601 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 map04110 map04111

Pfam Domains

Protein Families

Rad21_Rec8 Rad21_Rec8_N

Protein Analysis

541

Amino Acids

60.31

Weight (kDa)

7.74

Isoelectric Point (pI)

52.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad21_Rec8_N	PF04825	1 - 81	2.2e-26	N terminus of Rad21 / Rec8 like protein
Rad21_Rec8	PF04824	487 - 540	2.7e-20	Conserved region of Rad21 / Rec8 like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05490 AT5G05490
fragaria_vesca	FvH4_5g27761 FvH4_5g31060 FvH4_5g35270
malus_domestica	MD12G1179600.v1.1
prunus_persica	Prupe.2G254800_v2.0.a1 Prupe.2G254800_v2.0.a1
pyrus_communis	pycom12g16940
rosa_chinensis	RchiOBHm_Chr0c11g0499361 RchiOBHm_Chr0c11g0499371 RchiOBHm_Chr0c11g0499561 RchiOBHm_Chr0c11g0499571 RchiOBHm_Chr0c11g0499581 RchiOBHm_Chr5g0049691 RchiOBHm_Chr5g0049701 RchiOBHm_Chr7g0230591 RchiOBHm_Chr7g0230601 RchiOBHm_Chr7g0231161 RchiOBHm_Chr7g0231171 RchiOBHm_Chr7g0231181 RchiOBHm_Chr7g0236141 RchiOBHm_Chr7g0236431 RchiOBHm_Chr7g0236461 RchiOBHm_Chr7g0236501
rosa_laevigata	RLG00000001078 RLG00000001102 RLG00000001195 RLG00000001205 RLG00000001937 RLG00000030015 RLG00000030017
rosa_multiflora	Rmu_co8170892.1_g000001 Rmu_co8276489.1_g000001 Rmu_sc0000112.1_g000006 Rmu_sc0000768.1_g000037 Rmu_sc0002222.1_g000015 Rmu_sc0002449.1_g000012 Rmu_sc0002449.1_g000029 Rmu_sc0002905.1_g000001 Rmu_sc0003720.1_g000008 Rmu_sc0005578.1_g000004 Rmu_sc0010974.1_g000001 Rmu_sc0010983.1_g000001
rosa_roxburghii	Rroxscaffold_3G00225060 Rroxscaffold_3G00225070 Rroxscaffold_3G00225350 Rroxscaffold_3G00226600 Rroxscaffold_3G00229860
rosa_rugosa	Rorug07G0294500 Rorug07G0294600 Rorug07G0295300 Rorug07G0297000
rosa_samantha	Rh1AG102400 Rh5BG337600 Rh5DG349800 Rh7AG413200 Rh7AG440900 Rh7AG448200 Rh7AG448700 Rh7AG449500 Rh7AG451400 Rh7AG452700 Rh7AG452800 Rh7AG453000 Rh7AG453100 Rh7CG431600 Rh7CG432100 Rh7CG453400 Rh7CG469500 Rh7CG471500 Rh7DG368200 Rh7DG409000 Rh7DG430700 Rh7DG438900 Rh7DG440800
rosa_wichuraiana	Rw1G008170 Rw7G034070 Rw7G036650 Rw7G036670 Rw7G036710 Rw7G037350 Rw7G037530 Rw7G037600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	762
AccB7I	CCANNNNNTGG	1 cut(s)	1022
AccI	GTMKAC	3 cut(s)	195, 238, 651
AccII	CGCG	1 cut(s)	450
AciI	CCGC	6 cut(s)	82, 244, 308, 920, 1397, 1581
AclWI	GGATC	5 cut(s)	293, 665, 1040, 1149, 1206
AcoI	YGGCCR	2 cut(s)	1013, 1125
AcsI	RAATTY	2 cut(s)	542, 1138
AcuI	CTGAAG	2 cut(s)	660, 1176
AfaI	GTAC	3 cut(s)	768, 1059, 1293
AfiI	CCNNNNNNNGG	5 cut(s)	223, 1022, 1196, 1322, 1587
AflII	CTTAAG	1 cut(s)	1085
AflIII	ACRYGT	1 cut(s)	831
AgsI	TTSAA	6 cut(s)	133, 566, 626, 776, 965, 1262
AjiI	CACGTC	2 cut(s)	405, 818
AjnI	CCWGG	5 cut(s)	513, 640, 898, 1132, 1476
Alw21I	GWGCWC	3 cut(s)	417, 1169, 1462
Alw26I	GTCTC	6 cut(s)	164, 380, 565, 870, 922, 1442
AlwI	GGATC	5 cut(s)	293, 665, 1040, 1149, 1206
Ama87I	CYCGRG	1 cut(s)	1162
AoxI	GGCC	7 cut(s)	467, 516, 1013, 1125, 1226, 1324, 1610
ApeKI	GCWGC	4 cut(s)	56, 284, 476, 1514
ApoI	RAATTY	2 cut(s)	542, 1138
Asp700I	GAANNNNTTC	1 cut(s)	883
AspLEI	GCGC	1 cut(s)	711
AspS9I	GGNCC	8 cut(s)	467, 751, 902, 944, 1325, 1361, 1578, 1610
AsuC2I	CCSGG	3 cut(s)	166, 1243, 1328
AsuHPI	GGTGA	5 cut(s)	503, 879, 903, 1274, 1305
AvaI	CYCGRG	1 cut(s)	1162
AvaII	GGWCC	5 cut(s)	751, 902, 944, 1361, 1578
AxyI	CCTNAGG	1 cut(s)	1464
BalI	TGGCCA	1 cut(s)	1127
BanII	GRGCYC	2 cut(s)	1169, 1462
BarI	GAAGNNNNNNTAC	2 cut(s)	203, 235
BauI	CACGAG	1 cut(s)	687
BbsI	GAAGAC	1 cut(s)	987
Bbv12I	GWGCWC	3 cut(s)	417, 1169, 1462
BbvI	GCAGC	4 cut(s)	43, 296, 488, 1526
BccI	CCATC	3 cut(s)	730, 913, 1166
BceAI	ACGGC	1 cut(s)	1028
BcgI	CGANNNNNNTGC	2 cut(s)	73, 107
BciT130I	CCWGG	5 cut(s)	515, 642, 900, 1134, 1478
BclI	TGATCA	2 cut(s)	496, 1336
BcnI	CCSGG	3 cut(s)	166, 1243, 1328
BcoDI	GTCTC	6 cut(s)	164, 380, 565, 870, 922, 1442
BfaI	CTAG	3 cut(s)	23, 506, 582
BfmI	CTRYAG	1 cut(s)	474
BfrI	CTTAAG	1 cut(s)	1085
BfuAI	ACCTGC	1 cut(s)	762
BglII	AGATCT	2 cut(s)	114, 679
BisI	GCNGC	5 cut(s)	57, 245, 285, 477, 1515
BlpI	GCTNAGC	1 cut(s)	1168
BlsI	GCNGC	5 cut(s)	58, 246, 286, 478, 1516
BmcAI	AGTACT	1 cut(s)	768
Bme1390I	CCNGG	8 cut(s)	166, 515, 642, 900, 1134, 1243, 1328, 1478
Bme18I	GGWCC	5 cut(s)	751, 902, 944, 1361, 1578
BmeT110I	CYCGRG	1 cut(s)	1162
BmgBI	CACGTC	2 cut(s)	405, 818
BmgT120I	GGNCC	8 cut(s)	467, 751, 902, 944, 1325, 1361, 1578, 1610
BmiI	GGNNCC	4 cut(s)	144, 665, 988, 1502
BmrFI	CCNGG	8 cut(s)	166, 515, 642, 900, 1134, 1243, 1328, 1478
BmrI	ACTGGG	1 cut(s)	985
BmsI	GCATC	5 cut(s)	217, 617, 682, 1181, 1247
BmuI	ACTGGG	1 cut(s)	985
BoxI	GACNNNNGTC	1 cut(s)	907
BpiI	GAAGAC	1 cut(s)	987
Bpu10I	CCTNAGC	1 cut(s)	1317
Bpu1102I	GCTNAGC	1 cut(s)	1168
BpuMI	CCSGG	3 cut(s)	166, 1243, 1328
BsaBI	GATNNNNATC	3 cut(s)	456, 501, 1116
BsaJI	CCNNGG	5 cut(s)	147, 319, 898, 1242, 1476
BsaXI	ACNNNNNCTCC	2 cut(s)	807, 837
Bsc4I	CCNNNNNNNGG	5 cut(s)	223, 1022, 1196, 1322, 1587
Bse1I	ACTGG	3 cut(s)	796, 947, 991
Bse21I	CCTNAGG	1 cut(s)	1464
Bse8I	GATNNNNATC	3 cut(s)	456, 501, 1116
BseBI	CCWGG	5 cut(s)	515, 642, 900, 1134, 1478
BseDI	CCNNGG	5 cut(s)	147, 319, 898, 1242, 1476
BseGI	GGATG	6 cut(s)	232, 385, 843, 1158, 1238, 1561
BseJI	GATNNNNATC	3 cut(s)	456, 501, 1116
BseLI	CCNNNNNNNGG	5 cut(s)	223, 1022, 1196, 1322, 1587
BseMII	CTCAG	4 cut(s)	915, 1182, 1308, 1478
BseNI	ACTGG	3 cut(s)	796, 947, 991
BseRI	GAGGAG	3 cut(s)	27, 435, 1452
BseXI	GCAGC	4 cut(s)	43, 296, 488, 1526
Bsh1236I	CGCG	1 cut(s)	450
Bsh1285I	CGRYCG	1 cut(s)	1386
BshFI	GGCC	7 cut(s)	469, 518, 1015, 1127, 1228, 1326, 1612
BsiEI	CGRYCG	1 cut(s)	1386
BsiHKAI	GWGCWC	3 cut(s)	417, 1169, 1462
BsiHKCI	CYCGRG	1 cut(s)	1162
BsiSI	CCGG	5 cut(s)	165, 873, 1215, 1242, 1328
BslFI	GGGAC	1 cut(s)	592
BslI	CCNNNNNNNGG	5 cut(s)	223, 1022, 1196, 1322, 1587
BsmAI	GTCTC	6 cut(s)	164, 380, 565, 870, 922, 1442
BsmBI	CGTCTC	2 cut(s)	164, 1442
BsmFI	GGGAC	1 cut(s)	592
BsnI	GGCC	7 cut(s)	469, 518, 1015, 1127, 1228, 1326, 1612
BsoBI	CYCGRG	1 cut(s)	1162
Bsp1286I	GDGCHC	3 cut(s)	417, 1169, 1462
Bsp1407I	TGTACA	1 cut(s)	1291
Bsp1720I	GCTNAGC	1 cut(s)	1168
Bsp19I	CCATGG	1 cut(s)	147
Bsp68I	TCGCGA	1 cut(s)	450
BspACI	CCGC	6 cut(s)	82, 244, 308, 920, 1397, 1581
BspANI	GGCC	7 cut(s)	469, 518, 1015, 1127, 1228, 1326, 1612
BspCNI	CTCAG	4 cut(s)	916, 1181, 1309, 1477
BspFNI	CGCG	1 cut(s)	450
BspLI	GGNNCC	4 cut(s)	144, 665, 988, 1502
BspMAI	CTGCAG	1 cut(s)	478
BspMI	ACCTGC	1 cut(s)	762
BspPI	GGATC	5 cut(s)	293, 665, 1040, 1149, 1206
BspQI	GCTCTTC	2 cut(s)	159, 1529
BspTI	CTTAAG	1 cut(s)	1085
BsrGI	TGTACA	1 cut(s)	1291
BsrI	ACTGG	3 cut(s)	796, 947, 991
BssECI	CCNNGG	5 cut(s)	147, 319, 898, 1242, 1476
BssNAI	GTATAC	1 cut(s)	652
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	2 cut(s)	147, 319
Bst1107I	GTATAC	1 cut(s)	652
Bst2BI	CACGAG	1 cut(s)	687
Bst2UI	CCWGG	5 cut(s)	515, 642, 900, 1134, 1478
Bst4CI	ACNGT	1 cut(s)	393
Bst6I	CTCTTC	4 cut(s)	117, 159, 378, 1529
BstAFI	CTTAAG	1 cut(s)	1085
BstAUI	TGTACA	1 cut(s)	1291
BstC8I	GCNNGC	3 cut(s)	69, 606, 1182
BstDEI	CTNAG	5 cut(s)	924, 1099, 1168, 1317, 1464
BstDSI	CCRYGG	1 cut(s)	147
BstEII	GGTNACC	1 cut(s)	1311
BstENI	CCTNNNNNAGG	3 cut(s)	221, 1194, 1320
BstF5I	GGATG	6 cut(s)	232, 385, 843, 1158, 1238, 1561
BstFNI	CGCG	1 cut(s)	450
BstHHI	GCGC	1 cut(s)	711
BstMAI	GTCTC	6 cut(s)	164, 380, 565, 870, 922, 1442
BstMCI	CGRYCG	1 cut(s)	1386
BstMWI	GCNNNNNNNGC	2 cut(s)	32, 97
BstNI	CCWGG	5 cut(s)	515, 642, 900, 1134, 1478
BstNSI	RCATGY	1 cut(s)	835
BstPAI	GACNNNNGTC	1 cut(s)	907
BstPI	GGTNACC	1 cut(s)	1311
BstSCI	CCNGG	8 cut(s)	164, 513, 640, 898, 1132, 1241, 1326, 1476
BstSFI	CTRYAG	1 cut(s)	474
BstUI	CGCG	1 cut(s)	450
BstV1I	GCAGC	4 cut(s)	43, 296, 488, 1526
BstV2I	GAAGAC	1 cut(s)	987
BstX2I	RGATCY	5 cut(s)	114, 298, 670, 679, 1154
BstXI	CCANNNNNNTGG	1 cut(s)	1123
BstYI	RGATCY	5 cut(s)	114, 298, 670, 679, 1154
BstZ17I	GTATAC	1 cut(s)	652
Bsu36I	CCTNAGG	1 cut(s)	1464
BsuRI	GGCC	7 cut(s)	469, 518, 1015, 1127, 1228, 1326, 1612
BtgI	CCRYGG	1 cut(s)	147
BtgZI	GCGATG	1 cut(s)	465
BtrI	CACGTC	2 cut(s)	405, 818
BtsCI	GGATG	6 cut(s)	232, 385, 843, 1158, 1238, 1561
BtsIMutI	CAGTG	1 cut(s)	954
BtuMI	TCGCGA	1 cut(s)	450
BveI	ACCTGC	1 cut(s)	762
Cac8I	GCNNGC	3 cut(s)	69, 606, 1182
CfoI	GCGC	1 cut(s)	711
Cfr13I	GGNCC	8 cut(s)	467, 751, 902, 944, 1325, 1361, 1578, 1610
Csp6I	GTAC	3 cut(s)	767, 1058, 1292
CviAII	CATG	6 cut(s)	148, 175, 647, 748, 832, 1063
CviQI	GTAC	3 cut(s)	767, 1058, 1292
DdeI	CTNAG	5 cut(s)	924, 1099, 1168, 1317, 1464
EaeI	YGGCCR	2 cut(s)	1013, 1125
Eam1104I	CTCTTC	4 cut(s)	117, 159, 378, 1529
EarI	CTCTTC	4 cut(s)	117, 159, 378, 1529
Ecl136II	GAGCTC	2 cut(s)	1167, 1460
Eco130I	CCWWGG	2 cut(s)	147, 319
Eco147I	AGGCCT	1 cut(s)	1228
Eco24I	GRGCYC	2 cut(s)	1169, 1462
Eco47I	GGWCC	5 cut(s)	751, 902, 944, 1361, 1578
Eco53kI	GAGCTC	2 cut(s)	1167, 1460
Eco57I	CTGAAG	2 cut(s)	660, 1176
Eco81I	CCTNAGG	1 cut(s)	1464
Eco88I	CYCGRG	1 cut(s)	1162
Eco91I	GGTNACC	1 cut(s)	1311
EcoICRI	GAGCTC	2 cut(s)	1167, 1460
EcoNI	CCTNNNNNAGG	3 cut(s)	221, 1194, 1320
EcoO109I	RGGNCCY	1 cut(s)	467
EcoO65I	GGTNACC	1 cut(s)	1311
EcoRII	CCWGG	5 cut(s)	513, 640, 898, 1132, 1476
EcoT14I	CCWWGG	2 cut(s)	147, 319
EcoT38I	GRGCYC	2 cut(s)	1169, 1462
ErhI	CCWWGG	2 cut(s)	147, 319
Esp3I	CGTCTC	2 cut(s)	164, 1442
FaeI	CATG	6 cut(s)	151, 178, 650, 751, 835, 1066
FaqI	GGGAC	1 cut(s)	592
FatI	CATG	6 cut(s)	147, 174, 646, 747, 831, 1062
FbaI	TGATCA	2 cut(s)	496, 1336
FblI	GTMKAC	3 cut(s)	195, 238, 651
Fnu4HI	GCNGC	5 cut(s)	57, 245, 285, 477, 1515
FokI	GGATG	6 cut(s)	239, 392, 850, 1145, 1225, 1568
FriOI	GRGCYC	2 cut(s)	1169, 1462
Fsp4HI	GCNGC	5 cut(s)	57, 245, 285, 477, 1515
FspBI	CTAG	3 cut(s)	23, 506, 582
GlaI	GCGC	1 cut(s)	710
GluI	GCNGC	5 cut(s)	57, 245, 285, 477, 1515
HaeIII	GGCC	7 cut(s)	469, 518, 1015, 1127, 1228, 1326, 1612
HapII	CCGG	5 cut(s)	165, 873, 1215, 1242, 1328
HhaI	GCGC	1 cut(s)	711
Hin1II	CATG	6 cut(s)	151, 178, 650, 751, 835, 1066
Hin6I	GCGC	1 cut(s)	709
HinP1I	GCGC	1 cut(s)	709
HincII	GTYRAC	1 cut(s)	274
HindII	GTYRAC	1 cut(s)	274
HinfI	GANTC	9 cut(s)	133, 169, 183, 232, 863, 1279, 1472, 1566, 1594
HpaI	GTTAAC	1 cut(s)	274
HpaII	CCGG	5 cut(s)	165, 873, 1215, 1242, 1328
HphI	GGTGA	5 cut(s)	503, 879, 903, 1274, 1305
Hpy166II	GTNNAC	5 cut(s)	196, 239, 274, 652, 1058
Hpy188I	TCNGA	9 cut(s)	30, 343, 668, 679, 851, 1144, 1195, 1424, 1434
Hpy8I	GTNNAC	5 cut(s)	196, 239, 274, 652, 1058
HpyCH4III	ACNGT	1 cut(s)	393
HpyCH4IV	ACGT	4 cut(s)	404, 804, 817, 1149
HpyCH4V	TGCA	4 cut(s)	67, 476, 695, 1238
HpyF10VI	GCNNNNNNNGC	2 cut(s)	32, 97
HpyF3I	CTNAG	5 cut(s)	924, 1099, 1168, 1317, 1464
HpySE526I	ACGT	4 cut(s)	404, 804, 817, 1149
Hsp92II	CATG	6 cut(s)	151, 178, 650, 751, 835, 1066
HspAI	GCGC	1 cut(s)	709
Ksp22I	TGATCA	2 cut(s)	496, 1336
KspAI	GTTAAC	1 cut(s)	274
LguI	GCTCTTC	2 cut(s)	159, 1529
LmnI	GCTCC	7 cut(s)	40, 219, 310, 669, 992, 1457, 1465
Lsp1109I	GCAGC	4 cut(s)	43, 296, 488, 1526
LweI	GCATC	5 cut(s)	217, 617, 682, 1181, 1247
MaeI	CTAG	3 cut(s)	23, 506, 582
MaeII	ACGT	4 cut(s)	404, 804, 817, 1149
MaeIII	GTNAC	3 cut(s)	909, 1280, 1311
MboII	GAAGA	9 cut(s)	134, 146, 308, 326, 395, 745, 788, 987, 1516
MflI	RGATCY	5 cut(s)	114, 298, 670, 679, 1154
MhlI	GDGCHC	3 cut(s)	417, 1169, 1462
MlsI	TGGCCA	1 cut(s)	1127
MluCI	AATT	6 cut(s)	354, 542, 771, 1138, 1185, 1201
MluNI	TGGCCA	1 cut(s)	1127
MlyI	GAGTC	5 cut(s)	192, 226, 872, 1288, 1575
MmeI	TCCRAC	1 cut(s)	1073
Mox20I	TGGCCA	1 cut(s)	1127
MroXI	GAANNNNTTC	1 cut(s)	883
MscI	TGGCCA	1 cut(s)	1127
MseI	TTAA	4 cut(s)	273, 359, 396, 1086
MslI	CAYNNNNRTG	2 cut(s)	836, 1287
Msp20I	TGGCCA	1 cut(s)	1127
MspCI	CTTAAG	1 cut(s)	1085
MspI	CCGG	5 cut(s)	165, 873, 1215, 1242, 1328
MspR9I	CCNGG	8 cut(s)	166, 515, 642, 900, 1134, 1243, 1328, 1478
MvaI	CCWGG	5 cut(s)	515, 642, 900, 1134, 1478
MvnI	CGCG	1 cut(s)	450
MwoI	GCNNNNNNNGC	2 cut(s)	32, 97
NciI	CCSGG	3 cut(s)	166, 1243, 1328
NcoI	CCATGG	1 cut(s)	147
NlaIII	CATG	6 cut(s)	151, 178, 650, 751, 835, 1066
NlaIV	GGNNCC	4 cut(s)	144, 665, 988, 1502
NmuCI	GTSAC	3 cut(s)	909, 1280, 1311
NruI	TCGCGA	1 cut(s)	450
NspI	RCATGY	1 cut(s)	835
PceI	AGGCCT	1 cut(s)	1228
PciI	ACATGT	1 cut(s)	831
PciSI	GCTCTTC	2 cut(s)	159, 1529
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 1610
PdmI	GAANNNNTTC	1 cut(s)	883
PfeI	GAWTC	4 cut(s)	133, 169, 1472, 1594
PflMI	CCANNNNNTGG	1 cut(s)	1022
PfoI	TCCNGGA	1 cut(s)	164
PkrI	GCNGC	5 cut(s)	58, 246, 286, 478, 1516
Ple19I	CGATCG	1 cut(s)	1386
PleI	GAGTC	5 cut(s)	191, 226, 871, 1287, 1574
PpsI	GAGTC	5 cut(s)	191, 226, 871, 1287, 1574
PscI	ACATGT	1 cut(s)	831
PshAI	GACNNNNGTC	1 cut(s)	907
Psp124BI	GAGCTC	2 cut(s)	1169, 1462
Psp6I	CCWGG	5 cut(s)	513, 640, 898, 1132, 1476
PspEI	GGTNACC	1 cut(s)	1311
PspGI	CCWGG	5 cut(s)	513, 640, 898, 1132, 1476
PspN4I	GGNNCC	4 cut(s)	144, 665, 988, 1502
PspPI	GGNCC	8 cut(s)	467, 751, 902, 944, 1325, 1361, 1578, 1610
PstI	CTGCAG	1 cut(s)	478
PsuI	RGATCY	5 cut(s)	114, 298, 670, 679, 1154
PvuI	CGATCG	1 cut(s)	1386
RruI	TCGCGA	1 cut(s)	450
RsaI	GTAC	3 cut(s)	768, 1059, 1293
RsaNI	GTAC	3 cut(s)	767, 1058, 1292
RseI	CAYNNNNRTG	2 cut(s)	836, 1287
SacI	GAGCTC	2 cut(s)	1169, 1462
SapI	GCTCTTC	2 cut(s)	159, 1529
SaqAI	TTAA	4 cut(s)	273, 359, 396, 1086
SatI	GCNGC	5 cut(s)	57, 245, 285, 477, 1515
Sau96I	GGNCC	8 cut(s)	467, 751, 902, 944, 1325, 1361, 1578, 1610
ScaI	AGTACT	1 cut(s)	768
SchI	GAGTC	5 cut(s)	192, 226, 872, 1288, 1575
ScrFI	CCNGG	8 cut(s)	166, 515, 642, 900, 1134, 1243, 1328, 1478
SduI	GDGCHC	3 cut(s)	417, 1169, 1462
SfaNI	GCATC	5 cut(s)	217, 617, 682, 1181, 1247
SfcI	CTRYAG	1 cut(s)	474
SinI	GGWCC	5 cut(s)	751, 902, 944, 1361, 1578
SmiMI	CAYNNNNRTG	2 cut(s)	836, 1287
SmlI	CTYRAG	1 cut(s)	1085
SmoI	CTYRAG	1 cut(s)	1085
Sse9I	AATT	6 cut(s)	354, 542, 771, 1138, 1185, 1201
SseBI	AGGCCT	1 cut(s)	1228
SsiI	CCGC	6 cut(s)	82, 244, 308, 920, 1397, 1581
SspMI	CTAG	3 cut(s)	23, 506, 582
SstI	GAGCTC	2 cut(s)	1169, 1462
StuI	AGGCCT	1 cut(s)	1228
StyD4I	CCNGG	8 cut(s)	164, 513, 640, 898, 1132, 1241, 1326, 1476
StyI	CCWWGG	2 cut(s)	147, 319
TaaI	ACNGT	1 cut(s)	393
TaiI	ACGT	4 cut(s)	407, 807, 820, 1152
TaqI	TCGA	9 cut(s)	93, 102, 258, 372, 1299, 1307, 1470, 1569, 1604
TasI	AATT	6 cut(s)	354, 542, 771, 1138, 1185, 1201
TatI	WGTACW	3 cut(s)	766, 1057, 1291
TauI	GCSGC	1 cut(s)	247
TfiI	GAWTC	4 cut(s)	133, 169, 1472, 1594
Tru1I	TTAA	4 cut(s)	273, 359, 396, 1086
Tru9I	TTAA	4 cut(s)	273, 359, 396, 1086
TscAI	CASTG	1 cut(s)	954
TseFI	GTSAC	3 cut(s)	909, 1280, 1311
TseI	GCWGC	4 cut(s)	56, 284, 476, 1514
Tsp45I	GTSAC	3 cut(s)	909, 1280, 1311
TspDTI	ATGAA	7 cut(s)	276, 396, 468, 546, 569, 746, 854
TspGWI	ACGGA	1 cut(s)	1466
TspRI	CASTG	1 cut(s)	954
Van91I	CCANNNNNTGG	1 cut(s)	1022
Vha464I	CTTAAG	1 cut(s)	1085
VpaK11BI	GGWCC	5 cut(s)	751, 902, 944, 1361, 1578
XagI	CCTNNNNNAGG	3 cut(s)	221, 1194, 1320
XapI	RAATTY	2 cut(s)	542, 1138
XbaI	TCTAGA	1 cut(s)	505
XceI	RCATGY	1 cut(s)	835
XmiI	GTMKAC	3 cut(s)	195, 238, 651
XmnI	GAANNNNTTC	1 cut(s)	883
XspI	CTAG	3 cut(s)	23, 506, 582
ZrmI	AGTACT	1 cut(s)	768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.