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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 230 |
| AgsI | TTSAA | 1 cut(s) | 47 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 267, 299 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 178, 210 |
| AoxI | GGCC | 1 cut(s) | 300 |
| ApoI | RAATTY | 1 cut(s) | 230 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 164, 196 |
| AspLEI | GCGC | 1 cut(s) | 113 |
| AspS9I | GGNCC | 1 cut(s) | 186 |
| AvaII | GGWCC | 1 cut(s) | 186 |
| BanII | GRGCYC | 1 cut(s) | 292 |
| BbsI | GAAGAC | 1 cut(s) | 91 |
| BceAI | ACGGC | 1 cut(s) | 315 |
| BciVI | GTATCC | 1 cut(s) | 198 |
| BfmI | CTRYAG | 1 cut(s) | 103 |
| BfuI | GTATCC | 1 cut(s) | 198 |
| Bme18I | GGWCC | 1 cut(s) | 186 |
| BmgT120I | GGNCC | 1 cut(s) | 186 |
| BmiI | GGNNCC | 1 cut(s) | 158 |
| BpiI | GAAGAC | 1 cut(s) | 91 |
| BseGI | GGATG | 2 cut(s) | 251, 257 |
| Bsh1285I | CGRYCG | 1 cut(s) | 193 |
| BshFI | GGCC | 1 cut(s) | 302 |
| BsiEI | CGRYCG | 1 cut(s) | 193 |
| BsiSI | CCGG | 1 cut(s) | 21 |
| BsnI | GGCC | 1 cut(s) | 302 |
| Bsp1286I | GDGCHC | 1 cut(s) | 292 |
| Bsp143I | GATC | 1 cut(s) | 190 |
| BspANI | GGCC | 1 cut(s) | 302 |
| BspLI | GGNNCC | 1 cut(s) | 158 |
| BspMAI | CTGCAG | 1 cut(s) | 107 |
| BssMI | GATC | 1 cut(s) | 190 |
| Bst4CI | ACNGT | 1 cut(s) | 180 |
| Bst6I | CTCTTC | 1 cut(s) | 241 |
| BstF5I | GGATG | 2 cut(s) | 251, 257 |
| BstHHI | GCGC | 1 cut(s) | 113 |
| BstKTI | GATC | 1 cut(s) | 193 |
| BstMBI | GATC | 1 cut(s) | 190 |
| BstMCI | CGRYCG | 1 cut(s) | 193 |
| BstSFI | CTRYAG | 1 cut(s) | 103 |
| BstV2I | GAAGAC | 1 cut(s) | 91 |
| BsuI | GTATCC | 1 cut(s) | 198 |
| BsuRI | GGCC | 1 cut(s) | 302 |
| BtsCI | GGATG | 2 cut(s) | 251, 257 |
| BtsIMutI | CAGTG | 1 cut(s) | 158 |
| CfoI | GCGC | 1 cut(s) | 113 |
| Cfr13I | GGNCC | 1 cut(s) | 186 |
| CviAII | CATG | 1 cut(s) | 183 |
| CviJI | RGCY | 4 cut(s) | 54, 212, 290, 302 |
| CviKI_1 | RGCY | 4 cut(s) | 54, 212, 290, 302 |
| DpnI | GATC | 1 cut(s) | 192 |
| DpnII | GATC | 1 cut(s) | 190 |
| Eam1104I | CTCTTC | 1 cut(s) | 241 |
| EarI | CTCTTC | 1 cut(s) | 241 |
| Eco24I | GRGCYC | 1 cut(s) | 292 |
| Eco47I | GGWCC | 1 cut(s) | 186 |
| EcoT38I | GRGCYC | 1 cut(s) | 292 |
| FaeI | CATG | 1 cut(s) | 186 |
| FaiI | YATR | 6 cut(s) | 91, 96, 184, 202, 294, 312 |
| FatI | CATG | 1 cut(s) | 182 |
| FokI | GGATG | 2 cut(s) | 238, 244 |
| FriOI | GRGCYC | 1 cut(s) | 292 |
| GlaI | GCGC | 1 cut(s) | 112 |
| HaeIII | GGCC | 1 cut(s) | 302 |
| HapII | CCGG | 1 cut(s) | 21 |
| HhaI | GCGC | 1 cut(s) | 113 |
| Hin1II | CATG | 1 cut(s) | 186 |
| Hin6I | GCGC | 1 cut(s) | 111 |
| HinP1I | GCGC | 1 cut(s) | 111 |
| HpaII | CCGG | 1 cut(s) | 21 |
| HpyAV | CCTTC | 2 cut(s) | 41, 175 |
| HpyCH4III | ACNGT | 1 cut(s) | 180 |
| HpyCH4V | TGCA | 2 cut(s) | 105, 218 |
| Hsp92II | CATG | 1 cut(s) | 186 |
| HspAI | GCGC | 1 cut(s) | 111 |
| Kzo9I | GATC | 1 cut(s) | 190 |
| LmnI | GCTCC | 1 cut(s) | 143 |
| LpnPI | CCDG | 5 cut(s) | 34, 68, 114, 194, 249 |
| MaeIII | GTNAC | 2 cut(s) | 247, 306 |
| MalI | GATC | 1 cut(s) | 192 |
| MboI | GATC | 1 cut(s) | 190 |
| MboII | GAAGA | 2 cut(s) | 96, 228 |
| MfeI | CAATTG | 1 cut(s) | 145 |
| MhlI | GDGCHC | 1 cut(s) | 292 |
| MluCI | AATT | 3 cut(s) | 75, 145, 230 |
| MmeI | TCCRAC | 1 cut(s) | 165 |
| MnlI | CCTC | 1 cut(s) | 271 |
| MspI | CCGG | 1 cut(s) | 21 |
| MunI | CAATTG | 1 cut(s) | 145 |
| NdeII | GATC | 1 cut(s) | 190 |
| NlaIII | CATG | 1 cut(s) | 186 |
| NlaIV | GGNNCC | 1 cut(s) | 158 |
| NmuCI | GTSAC | 2 cut(s) | 247, 306 |
| Ple19I | CGATCG | 1 cut(s) | 193 |
| PspN4I | GGNNCC | 1 cut(s) | 158 |
| PspPI | GGNCC | 1 cut(s) | 186 |
| PstI | CTGCAG | 1 cut(s) | 107 |
| PvuI | CGATCG | 1 cut(s) | 193 |
| Sau3AI | GATC | 1 cut(s) | 190 |
| Sau96I | GGNCC | 1 cut(s) | 186 |
| SduI | GDGCHC | 1 cut(s) | 292 |
| SetI | ASST | 1 cut(s) | 162 |
| SfcI | CTRYAG | 1 cut(s) | 103 |
| SinI | GGWCC | 1 cut(s) | 186 |
| Sse9I | AATT | 3 cut(s) | 75, 145, 230 |
| TaaI | ACNGT | 1 cut(s) | 180 |
| TaqII | GACCGA | 1 cut(s) | 203 |
| TasI | AATT | 3 cut(s) | 75, 145, 230 |
| TscAI | CASTG | 1 cut(s) | 158 |
| TseFI | GTSAC | 2 cut(s) | 247, 306 |
| Tsp45I | GTSAC | 2 cut(s) | 247, 306 |
| TspRI | CASTG | 1 cut(s) | 158 |
| VpaK11BI | GGWCC | 1 cut(s) | 186 |
| XapI | RAATTY | 1 cut(s) | 230 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 125 |