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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 299 |
| AcsI | RAATTY | 1 cut(s) | 145 |
| AfaI | GTAC | 1 cut(s) | 314 |
| AflIII | ACRYGT | 1 cut(s) | 258 |
| AgsI | TTSAA | 1 cut(s) | 47 |
| AluBI | AGCT | 1 cut(s) | 204 |
| AluI | AGCT | 1 cut(s) | 204 |
| Alw21I | GWGCWC | 1 cut(s) | 115 |
| Alw44I | GTGCAC | 1 cut(s) | 111 |
| AoxI | GGCC | 1 cut(s) | 86 |
| ApaLI | GTGCAC | 1 cut(s) | 111 |
| ApoI | RAATTY | 1 cut(s) | 145 |
| BaeGI | GKGCMC | 1 cut(s) | 115 |
| Bbv12I | GWGCWC | 1 cut(s) | 115 |
| BlpI | GCTNAGC | 1 cut(s) | 214 |
| BmsI | GCATC | 1 cut(s) | 286 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 214 |
| BsaJI | CCNNGG | 2 cut(s) | 9, 126 |
| BsaWI | WCCGGW | 1 cut(s) | 20 |
| Bse3DI | GCAATG | 1 cut(s) | 304 |
| BseDI | CCNNGG | 2 cut(s) | 9, 126 |
| BseGI | GGATG | 2 cut(s) | 266, 301 |
| BseMI | GCAATG | 1 cut(s) | 304 |
| BseMII | CTCAG | 1 cut(s) | 170 |
| BseSI | GKGCMC | 1 cut(s) | 115 |
| BseYI | CCCAGC | 1 cut(s) | 251 |
| BsgI | GTGCAG | 1 cut(s) | 101 |
| BshFI | GGCC | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 1 cut(s) | 115 |
| BsiSI | CCGG | 1 cut(s) | 21 |
| BsmI | GAATGC | 1 cut(s) | 241 |
| BsnI | GGCC | 1 cut(s) | 88 |
| Bsp1286I | GDGCHC | 1 cut(s) | 115 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 214 |
| BspACI | CCGC | 1 cut(s) | 299 |
| BspANI | GGCC | 1 cut(s) | 88 |
| BspCNI | CTCAG | 1 cut(s) | 169 |
| BsrDI | GCAATG | 1 cut(s) | 304 |
| BssECI | CCNNGG | 2 cut(s) | 9, 126 |
| BssT1I | CCWWGG | 2 cut(s) | 9, 126 |
| Bst4CI | ACNGT | 1 cut(s) | 110 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 138 |
| BstC8I | GCNNGC | 1 cut(s) | 139 |
| BstDEI | CTNAG | 3 cut(s) | 156, 214, 237 |
| BstF5I | GGATG | 2 cut(s) | 266, 301 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 138, 210 |
| BstSLI | GKGCMC | 1 cut(s) | 115 |
| BsuRI | GGCC | 1 cut(s) | 88 |
| BtsCI | GGATG | 2 cut(s) | 266, 301 |
| BtsI | GCAGTG | 2 cut(s) | 113, 139 |
| BtsIMutI | CAGTG | 3 cut(s) | 41, 113, 139 |
| Cac8I | GCNNGC | 1 cut(s) | 139 |
| Csp6I | GTAC | 1 cut(s) | 313 |
| CviAII | CATG | 1 cut(s) | 192 |
| CviJI | RGCY | 4 cut(s) | 54, 88, 204, 218 |
| CviKI_1 | RGCY | 4 cut(s) | 54, 88, 204, 218 |
| CviQI | GTAC | 1 cut(s) | 313 |
| DdeI | CTNAG | 3 cut(s) | 156, 214, 237 |
| Eco130I | CCWWGG | 2 cut(s) | 9, 126 |
| EcoRI | GAATTC | 1 cut(s) | 145 |
| EcoT14I | CCWWGG | 2 cut(s) | 9, 126 |
| ErhI | CCWWGG | 2 cut(s) | 9, 126 |
| FaeI | CATG | 1 cut(s) | 195 |
| FaiI | YATR | 3 cut(s) | 91, 96, 193 |
| FatI | CATG | 1 cut(s) | 191 |
| FokI | GGATG | 2 cut(s) | 253, 308 |
| GsaI | CCCAGC | 1 cut(s) | 255 |
| HaeIII | GGCC | 1 cut(s) | 88 |
| HapII | CCGG | 1 cut(s) | 21 |
| Hin1II | CATG | 1 cut(s) | 195 |
| HinfI | GANTC | 1 cut(s) | 224 |
| HpaII | CCGG | 1 cut(s) | 21 |
| Hpy166II | GTNNAC | 2 cut(s) | 113, 264 |
| Hpy8I | GTNNAC | 2 cut(s) | 113, 264 |
| HpyAV | CCTTC | 3 cut(s) | 41, 58, 78 |
| HpyCH4III | ACNGT | 1 cut(s) | 110 |
| HpyCH4IV | ACGT | 1 cut(s) | 260 |
| HpyCH4V | TGCA | 3 cut(s) | 105, 113, 118 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 138, 210 |
| HpyF3I | CTNAG | 3 cut(s) | 156, 214, 237 |
| HpySE526I | ACGT | 1 cut(s) | 260 |
| Hsp92II | CATG | 1 cut(s) | 195 |
| LmnI | GCTCC | 2 cut(s) | 165, 209 |
| LpnPI | CCDG | 3 cut(s) | 34, 68, 265 |
| LweI | GCATC | 1 cut(s) | 286 |
| MaeII | ACGT | 1 cut(s) | 260 |
| MboII | GAAGA | 1 cut(s) | 225 |
| MhlI | GDGCHC | 1 cut(s) | 115 |
| MluCI | AATT | 2 cut(s) | 75, 145 |
| MseI | TTAA | 1 cut(s) | 26 |
| MspI | CCGG | 1 cut(s) | 21 |
| Mva1269I | GAATGC | 1 cut(s) | 241 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 138, 210 |
| NlaIII | CATG | 1 cut(s) | 195 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 179 |
| PctI | GAATGC | 1 cut(s) | 241 |
| PfeI | GAWTC | 1 cut(s) | 224 |
| PspFI | CCCAGC | 1 cut(s) | 251 |
| RsaI | GTAC | 1 cut(s) | 314 |
| RsaNI | GTAC | 1 cut(s) | 313 |
| SaqAI | TTAA | 1 cut(s) | 26 |
| SduI | GDGCHC | 1 cut(s) | 115 |
| SetI | ASST | 3 cut(s) | 11, 206, 263 |
| SfaNI | GCATC | 1 cut(s) | 286 |
| Sse9I | AATT | 2 cut(s) | 75, 145 |
| SsiI | CCGC | 1 cut(s) | 299 |
| StyI | CCWWGG | 2 cut(s) | 9, 126 |
| TaaI | ACNGT | 1 cut(s) | 110 |
| TaiI | ACGT | 1 cut(s) | 263 |
| TaqI | TCGA | 1 cut(s) | 143 |
| TasI | AATT | 2 cut(s) | 75, 145 |
| TfiI | GAWTC | 1 cut(s) | 224 |
| Tru1I | TTAA | 1 cut(s) | 26 |
| Tru9I | TTAA | 1 cut(s) | 26 |
| TscAI | CASTG | 3 cut(s) | 41, 120, 139 |
| TspDTI | ATGAA | 2 cut(s) | 77, 293 |
| TspRI | CASTG | 3 cut(s) | 41, 120, 139 |
| VneI | GTGCAC | 1 cut(s) | 111 |
| XapI | RAATTY | 1 cut(s) | 145 |