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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 251 |
| AclWI | GGATC | 1 cut(s) | 211 |
| AgsI | TTSAA | 1 cut(s) | 47 |
| AjnI | CCWGG | 1 cut(s) | 247 |
| AluBI | AGCT | 1 cut(s) | 17 |
| AluI | AGCT | 1 cut(s) | 17 |
| Alw21I | GWGCWC | 1 cut(s) | 115 |
| Alw26I | GTCTC | 1 cut(s) | 312 |
| Alw44I | GTGCAC | 1 cut(s) | 111 |
| AlwI | GGATC | 1 cut(s) | 211 |
| ApaLI | GTGCAC | 1 cut(s) | 111 |
| BaeGI | GKGCMC | 1 cut(s) | 115 |
| BanI | GGYRCC | 1 cut(s) | 251 |
| BbsI | GAAGAC | 1 cut(s) | 91 |
| Bbv12I | GWGCWC | 1 cut(s) | 115 |
| BccI | CCATC | 2 cut(s) | 194, 275 |
| BciT130I | CCWGG | 1 cut(s) | 249 |
| BcoDI | GTCTC | 1 cut(s) | 312 |
| Bme1390I | CCNGG | 1 cut(s) | 249 |
| BmiI | GGNNCC | 1 cut(s) | 253 |
| BmrFI | CCNGG | 1 cut(s) | 249 |
| BmsI | GCATC | 1 cut(s) | 136 |
| BpiI | GAAGAC | 1 cut(s) | 91 |
| BseBI | CCWGG | 1 cut(s) | 249 |
| BseSI | GKGCMC | 1 cut(s) | 115 |
| BsgI | GTGCAG | 1 cut(s) | 101 |
| BshNI | GGYRCC | 1 cut(s) | 251 |
| BsiHKAI | GWGCWC | 1 cut(s) | 115 |
| BsmAI | GTCTC | 1 cut(s) | 312 |
| Bsp1286I | GDGCHC | 1 cut(s) | 115 |
| Bsp143I | GATC | 1 cut(s) | 203 |
| BspLI | GGNNCC | 1 cut(s) | 253 |
| BspPI | GGATC | 1 cut(s) | 211 |
| BspT107I | GGYRCC | 1 cut(s) | 251 |
| BssMI | GATC | 1 cut(s) | 203 |
| Bst2UI | CCWGG | 1 cut(s) | 249 |
| BstKTI | GATC | 1 cut(s) | 206 |
| BstMAI | GTCTC | 1 cut(s) | 312 |
| BstMBI | GATC | 1 cut(s) | 203 |
| BstNI | CCWGG | 1 cut(s) | 249 |
| BstNSI | RCATGY | 1 cut(s) | 217 |
| BstSCI | CCNGG | 1 cut(s) | 247 |
| BstSLI | GKGCMC | 1 cut(s) | 115 |
| BstV2I | GAAGAC | 1 cut(s) | 91 |
| BtgZI | GCGATG | 1 cut(s) | 159 |
| BtsI | GCAGTG | 1 cut(s) | 113 |
| BtsIMutI | CAGTG | 1 cut(s) | 113 |
| CviAII | CATG | 3 cut(s) | 25, 192, 214 |
| CviJI | RGCY | 2 cut(s) | 17, 54 |
| CviKI_1 | RGCY | 2 cut(s) | 17, 54 |
| DpnI | GATC | 1 cut(s) | 205 |
| DpnII | GATC | 1 cut(s) | 203 |
| EcoRII | CCWGG | 1 cut(s) | 247 |
| FaeI | CATG | 3 cut(s) | 28, 195, 217 |
| FaiI | YATR | 7 cut(s) | 26, 91, 96, 193, 215, 263, 278 |
| FatI | CATG | 3 cut(s) | 24, 191, 213 |
| Hin1II | CATG | 3 cut(s) | 28, 195, 217 |
| Hpy166II | GTNNAC | 1 cut(s) | 113 |
| Hpy188I | TCNGA | 1 cut(s) | 272 |
| Hpy8I | GTNNAC | 1 cut(s) | 113 |
| HpyAV | CCTTC | 2 cut(s) | 41, 184 |
| HpyCH4V | TGCA | 5 cut(s) | 64, 105, 113, 118, 213 |
| Hsp92II | CATG | 3 cut(s) | 28, 195, 217 |
| Kzo9I | GATC | 1 cut(s) | 203 |
| LmnI | GCTCC | 1 cut(s) | 14 |
| LpnPI | CCDG | 5 cut(s) | 6, 50, 163, 234, 261 |
| LweI | GCATC | 1 cut(s) | 136 |
| MalI | GATC | 1 cut(s) | 205 |
| MboI | GATC | 1 cut(s) | 203 |
| MboII | GAAGA | 1 cut(s) | 96 |
| MfeI | CAATTG | 1 cut(s) | 106 |
| MhlI | GDGCHC | 1 cut(s) | 115 |
| MluCI | AATT | 2 cut(s) | 75, 106 |
| MnlI | CCTC | 4 cut(s) | 123, 165, 247, 255 |
| MseI | TTAA | 1 cut(s) | 288 |
| MslI | CAYNNNNRTG | 1 cut(s) | 260 |
| MspR9I | CCNGG | 1 cut(s) | 249 |
| MunI | CAATTG | 1 cut(s) | 106 |
| MvaI | CCWGG | 1 cut(s) | 249 |
| NdeII | GATC | 1 cut(s) | 203 |
| NlaIII | CATG | 3 cut(s) | 28, 195, 217 |
| NlaIV | GGNNCC | 1 cut(s) | 253 |
| NspI | RCATGY | 1 cut(s) | 217 |
| Psp6I | CCWGG | 1 cut(s) | 247 |
| PspGI | CCWGG | 1 cut(s) | 247 |
| PspN4I | GGNNCC | 1 cut(s) | 253 |
| RseI | CAYNNNNRTG | 1 cut(s) | 260 |
| SaqAI | TTAA | 1 cut(s) | 288 |
| Sau3AI | GATC | 1 cut(s) | 203 |
| ScrFI | CCNGG | 1 cut(s) | 249 |
| SduI | GDGCHC | 1 cut(s) | 115 |
| SetI | ASST | 2 cut(s) | 11, 19 |
| SfaNI | GCATC | 1 cut(s) | 136 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 260 |
| Sse9I | AATT | 2 cut(s) | 75, 106 |
| StyD4I | CCNGG | 1 cut(s) | 247 |
| TasI | AATT | 2 cut(s) | 75, 106 |
| Tru1I | TTAA | 1 cut(s) | 288 |
| Tru9I | TTAA | 1 cut(s) | 288 |
| TscAI | CASTG | 1 cut(s) | 120 |
| TspRI | CASTG | 1 cut(s) | 120 |
| VneI | GTGCAC | 1 cut(s) | 111 |
| XceI | RCATGY | 1 cut(s) | 217 |