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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 47 |
| AjnI | CCWGG | 1 cut(s) | 244 |
| Alw21I | GWGCWC | 1 cut(s) | 115 |
| AoxI | GGCC | 1 cut(s) | 86 |
| ApeKI | GCWGC | 1 cut(s) | 218 |
| AseI | ATTAAT | 1 cut(s) | 162 |
| Bbv12I | GWGCWC | 1 cut(s) | 115 |
| BbvI | GCAGC | 1 cut(s) | 230 |
| BccI | CCATC | 2 cut(s) | 191, 244 |
| BciT130I | CCWGG | 1 cut(s) | 246 |
| BisI | GCNGC | 1 cut(s) | 219 |
| BlsI | GCNGC | 1 cut(s) | 220 |
| Bme1390I | CCNGG | 1 cut(s) | 246 |
| BmiI | GGNNCC | 1 cut(s) | 202 |
| BmrFI | CCNGG | 1 cut(s) | 246 |
| BsaJI | CCNNGG | 1 cut(s) | 9 |
| Bse1I | ACTGG | 1 cut(s) | 34 |
| BseBI | CCWGG | 1 cut(s) | 246 |
| BseDI | CCNNGG | 1 cut(s) | 9 |
| BseGI | GGATG | 3 cut(s) | 30, 241, 254 |
| BseNI | ACTGG | 1 cut(s) | 34 |
| BseXI | GCAGC | 1 cut(s) | 230 |
| BsgI | GTGCAG | 1 cut(s) | 101 |
| BshFI | GGCC | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 1 cut(s) | 115 |
| BslFI | GGGAC | 1 cut(s) | 218 |
| BsmFI | GGGAC | 1 cut(s) | 218 |
| BsnI | GGCC | 1 cut(s) | 88 |
| Bsp1286I | GDGCHC | 1 cut(s) | 115 |
| BspANI | GGCC | 1 cut(s) | 88 |
| BspLI | GGNNCC | 1 cut(s) | 202 |
| BsrI | ACTGG | 1 cut(s) | 34 |
| BssECI | CCNNGG | 1 cut(s) | 9 |
| BssT1I | CCWWGG | 1 cut(s) | 9 |
| Bst2UI | CCWGG | 1 cut(s) | 246 |
| BstC8I | GCNNGC | 1 cut(s) | 139 |
| BstF5I | GGATG | 3 cut(s) | 30, 241, 254 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 207 |
| BstNI | CCWGG | 1 cut(s) | 246 |
| BstNSI | RCATGY | 1 cut(s) | 214 |
| BstSCI | CCNGG | 1 cut(s) | 244 |
| BstV1I | GCAGC | 1 cut(s) | 230 |
| BsuRI | GGCC | 1 cut(s) | 88 |
| BtsCI | GGATG | 3 cut(s) | 30, 241, 254 |
| BtsIMutI | CAGTG | 1 cut(s) | 41 |
| Cac8I | GCNNGC | 1 cut(s) | 139 |
| CviAII | CATG | 2 cut(s) | 189, 211 |
| CviJI | RGCY | 5 cut(s) | 16, 54, 88, 201, 338 |
| CviKI_1 | RGCY | 5 cut(s) | 16, 54, 88, 201, 338 |
| Eco130I | CCWWGG | 1 cut(s) | 9 |
| EcoRII | CCWGG | 1 cut(s) | 244 |
| EcoT14I | CCWWGG | 1 cut(s) | 9 |
| EcoT22I | ATGCAT | 1 cut(s) | 212 |
| ErhI | CCWWGG | 1 cut(s) | 9 |
| FaeI | CATG | 2 cut(s) | 192, 214 |
| FaiI | YATR | 7 cut(s) | 91, 96, 190, 208, 212, 312, 321 |
| FaqI | GGGAC | 1 cut(s) | 218 |
| FatI | CATG | 2 cut(s) | 188, 210 |
| Fnu4HI | GCNGC | 1 cut(s) | 219 |
| FokI | GGATG | 3 cut(s) | 37, 228, 241 |
| Fsp4HI | GCNGC | 1 cut(s) | 219 |
| GluI | GCNGC | 1 cut(s) | 219 |
| HaeIII | GGCC | 1 cut(s) | 88 |
| Hin1II | CATG | 2 cut(s) | 192, 214 |
| Hpy188I | TCNGA | 1 cut(s) | 334 |
| HpyAV | CCTTC | 2 cut(s) | 41, 181 |
| HpyCH4V | TGCA | 4 cut(s) | 64, 105, 118, 210 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 207 |
| Hsp92II | CATG | 2 cut(s) | 192, 214 |
| LmnI | GCTCC | 1 cut(s) | 206 |
| LpnPI | CCDG | 8 cut(s) | 6, 47, 50, 68, 106, 231, 258, 280 |
| Lsp1109I | GCAGC | 1 cut(s) | 230 |
| MaeIII | GTNAC | 1 cut(s) | 306 |
| MhlI | GDGCHC | 1 cut(s) | 115 |
| MluCI | AATT | 2 cut(s) | 75, 159 |
| MnlI | CCTC | 2 cut(s) | 165, 328 |
| Mph1103I | ATGCAT | 1 cut(s) | 212 |
| MseI | TTAA | 2 cut(s) | 162, 343 |
| MspR9I | CCNGG | 1 cut(s) | 246 |
| MvaI | CCWGG | 1 cut(s) | 246 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 207 |
| NlaIII | CATG | 2 cut(s) | 192, 214 |
| NlaIV | GGNNCC | 1 cut(s) | 202 |
| NmuCI | GTSAC | 1 cut(s) | 306 |
| NsiI | ATGCAT | 1 cut(s) | 212 |
| NspI | RCATGY | 1 cut(s) | 214 |
| PfoI | TCCNGGA | 1 cut(s) | 244 |
| PkrI | GCNGC | 1 cut(s) | 220 |
| PshBI | ATTAAT | 1 cut(s) | 162 |
| Psp6I | CCWGG | 1 cut(s) | 244 |
| PspGI | CCWGG | 1 cut(s) | 244 |
| PspN4I | GGNNCC | 1 cut(s) | 202 |
| SaqAI | TTAA | 2 cut(s) | 162, 343 |
| SatI | GCNGC | 1 cut(s) | 219 |
| ScrFI | CCNGG | 1 cut(s) | 246 |
| SduI | GDGCHC | 1 cut(s) | 115 |
| SetI | ASST | 2 cut(s) | 11, 125 |
| Sse9I | AATT | 2 cut(s) | 75, 159 |
| SspI | AATATT | 1 cut(s) | 166 |
| StyD4I | CCNGG | 1 cut(s) | 244 |
| StyI | CCWWGG | 1 cut(s) | 9 |
| TaqI | TCGA | 1 cut(s) | 125 |
| TasI | AATT | 2 cut(s) | 75, 159 |
| Tru1I | TTAA | 2 cut(s) | 162, 343 |
| Tru9I | TTAA | 2 cut(s) | 162, 343 |
| TscAI | CASTG | 1 cut(s) | 41 |
| TseFI | GTSAC | 1 cut(s) | 306 |
| TseI | GCWGC | 1 cut(s) | 218 |
| Tsp45I | GTSAC | 1 cut(s) | 306 |
| TspRI | CASTG | 1 cut(s) | 41 |
| VspI | ATTAAT | 1 cut(s) | 162 |
| XceI | RCATGY | 1 cut(s) | 214 |
| Zsp2I | ATGCAT | 1 cut(s) | 212 |