MD16G1111100.v1.1

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000502
Physical Location & Seq
Forward (+)
7801943 .. 7805303
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1111100.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCAACATCTGGCAAGAAGATCAAGATCGGAATCAACGGGTTCGGAAGAATCGGACGATTGGTTGCTAGGGTCGCTCTTCAGAGGGACGATGTTGAGCTCGTTGCTGTCAACGATCCCTTCATCACCACCGACTACATGACCTACATGTTCAAGTACGACACAGTCCATGGCGCATGGAAGCACCATGAGCTCAAGGTCAAGGACTCCAAGACCCTTCTCTTCGGTGAGAAGCCTGTCGCTGTTTTCGGAATCAGGAACCCAGAGGAGATCCCATGGGGTGAGGCCGGTGCTGACTTTGTTGTTGAGTCTACCGGTGTTTTCACCGACAAGGAGAAAGCTGCTGCCCATATCAAGGGAGGTGCAAAGAAGGTTATCATCTCTGCTCCCAGCAAGGATGCTCCCATGTTCGTTGTTGGTGTGAACGAGAAGGAATACAAGCCAGACATTCACATTCTTTCCAATGCCAGTTGCACTACCAACTGCCTTGCTCCCCTTGCCAAGGTTATCAACGACAGGTTTGGAATTGTTGAGGGTCTCATGACCACGGTGCACTCCATCACTGCCACCCAAAAGACTGTTGACGGTCCATCAATGAAGGACTGGAGAGGTGGACGTGCAGCTTCCTTCAACATCATTCCTAGTAGCACTGGAGCTGCCAAGGCTGTTGGAAAGGTGCTCCCATCTCTTAATGGAAAATTGACCGGAATGTCCTTCCGTGTGCCCACTGTTGATGTTTCCGTTGTTGACCTGACTGTCAGGCTTGAGAAGGCTGCAACCTATGAACAGATCAAGGCAGCTATCAAGGAGGAGTCTGAGGGCAAGTTGAAGGGTATCTTGGGTTACACCGAAGATGATGTCGTGTCCACCGACTTCATTGGTGACAGCAGGTCAAGCATCTTTGATGCCAAGGCTGGAATTGCATTGAATGACAACTTTGTCAAGCTTGTCTCATGGTACGACAACGAGTGGGGTTACAGTTCCCGTGTGGTAGACTTGATCGTGCACGTAGCATCGAGTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04066 ko05010 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230 map04066 map05010
Pfam Domains
Protein Families
Gp_dh_C Gp_dh_N

Protein Analysis

342

Amino Acids

36.93

Weight (kDa)

7.72

Isoelectric Point (pI)

24.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gp_dh_N PF00044 8 - 111 1e-33 Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
Gp_dh_C PF02800 163 - 320 3e-71 Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13440 AT1G13440 AT3G04120
fragaria_vesca FvH4_4g24420 FvH4_5g07640 FvH4_6g52741 FvH4_6g52755
malus_domestica MD06G1148800.v1.1 MD09G1016900.v1.1 MD13G1111500.v1.1 MD16G1111100.v1.1 MD17G1017300.v1.1
prunus_persica Prupe.1G234000_v2.0.a1 Prupe.1G234000_v2.0.a1 Prupe.1G234000_v2.0.a1 Prupe.3G300600_v2.0.a1 Prupe.5G155800_v2.0.a1 Prupe.5G155800_v2.0.a1
pyrus_communis pycom06g13820 pycom111g01370 pycom13g09680 pycom16g09410
rosa_chinensis RchiOBHm_Chr1g0380411 RchiOBHm_Chr2g0174371 RchiOBHm_Chr2g0174391 RchiOBHm_Chr2g0174401 RchiOBHm_Chr4g0431271 RchiOBHm_Chr7g0190831 RchiOBHm_Chr7g0209601
rosa_laevigata RLG00000003113 RLG00000004459 RLG00000004490 RLG00000022261 RLG00000022262 RLG00000026305
rosa_multiflora Rmu_sc0000033.1_g000020 Rmu_sc0000243.1_g000023 Rmu_sc0002637.1_g000006 Rmu_sc0002637.1_g000007 Rmu_sc0004033.1_g000020 Rmu_sc0004787.1_g000001 Rmu_sc0005371.1_g000004 Rmu_sc0007885.1_g000015 Rmu_sc0019128.1_g000001
rosa_roxburghii Rroxscaffold_2G00078250 Rroxscaffold_2G00078260 Rroxscaffold_3G00249220 Rroxscaffold_3G00264260 Rroxscaffold_4G00279410 Rroxscaffold_5G00372820
rosa_rugosa Rorug01G0420800 Rorug04G0249800 Rorug04G0249900 Rorug06G0507500 Rorug07G0114500.1
rosa_samantha Rh1AG445000 Rh1BG400200 Rh1CG414800 Rh1DG430100 Rh2AG657500 Rh2AG657600 Rh2BG668500 Rh2BG668600 Rh2BG668700 Rh2CG631600 Rh2DG682100 Rh4AG305700 Rh4BG313100 Rh4CG329300 Rh4DG309500 Rh5AG149300 Rh5DG147700 Rh7AG114100 Rh7AG248500 Rh7BG116700 Rh7BG242600 Rh7CG119400 Rh7CG264500 Rh7DG117900 Rh7DG254400
rosa_wichuraiana Rw1G038730 Rw2G053850 Rw2G053860 Rw4G026580 Rw5G013240 Rw7G021060 Rw7G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 709, 755, 938
Acc36I ACCTGC 1 cut(s) 879
AccI GTMKAC 2 cut(s) 311, 993
AclWI GGATC 2 cut(s) 110, 265
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 158, 959
AfiI CCNNNNNNNGG 2 cut(s) 355, 502
AflIII ACRYGT 1 cut(s) 147
AgeI ACCGGT 1 cut(s) 314
AgsI TTSAA 4 cut(s) 154, 631, 829, 928
AhdI GACNNNNNGTC 1 cut(s) 889
AjiI CACGTC 1 cut(s) 617
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
AluBI AGCT 7 cut(s) 100, 193, 341, 623, 656, 800, 946
AluI AGCT 7 cut(s) 100, 193, 341, 623, 656, 800, 946
Alw21I GWGCWC 5 cut(s) 102, 195, 555, 681, 1008
Alw26I GTCTC 2 cut(s) 542, 955
Alw44I GTGCAC 2 cut(s) 551, 1004
AlwI GGATC 2 cut(s) 110, 265
AoxI GGCC 1 cut(s) 285
ApaLI GTGCAC 2 cut(s) 551, 1004
ApeKI GCWGC 6 cut(s) 341, 344, 620, 656, 773, 797
ArsI GACNNNNNNTTYG 2 cut(s) 843, 875
AsiGI ACCGGT 1 cut(s) 314
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 5 cut(s) 118, 239, 293, 316, 893
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 3 cut(s) 555, 726, 1008
BanII GRGCYC 2 cut(s) 102, 195
Bbv12I GWGCWC 5 cut(s) 102, 195, 555, 681, 1008
BbvI GCAGC 6 cut(s) 328, 331, 632, 643, 760, 809
BccI CCATC 3 cut(s) 566, 598, 691
BcoDI GTCTC 2 cut(s) 542, 955
BfaI CTAG 3 cut(s) 69, 642, 1024
BfuAI ACCTGC 1 cut(s) 879
BisI GCNGC 6 cut(s) 342, 345, 621, 657, 774, 798
BlsI GCNGC 6 cut(s) 343, 346, 622, 658, 775, 799
Bme18I GGWCC 1 cut(s) 587
BmeRI GACNNNNNGTC 1 cut(s) 889
BmgBI CACGTC 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 260
BmsI GCATC 4 cut(s) 388, 895, 906, 1022
BpmI CTGGAG 2 cut(s) 625, 672
BpuEI CTTGAG 2 cut(s) 179, 785
BsaAI YACGTR 1 cut(s) 1009
BsaBI GATNNNNATC 2 cut(s) 26, 32
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 6 cut(s) 169, 275, 501, 546, 660, 909
BsaWI WCCGGW 2 cut(s) 314, 704
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 502
Bse118I RCCGGY 2 cut(s) 287, 314
Bse1I ACTGG 3 cut(s) 468, 608, 655
Bse8I GATNNNNATC 2 cut(s) 26, 32
BseDI CCNNGG 6 cut(s) 169, 275, 501, 546, 660, 909
BseGI GGATG 1 cut(s) 403
BseJI GATNNNNATC 2 cut(s) 26, 32
BseLI CCNNNNNNNGG 2 cut(s) 355, 502
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 3 cut(s) 468, 608, 655
BseRI GAGGAG 2 cut(s) 281, 824
BseSI GKGCMC 3 cut(s) 555, 726, 1008
BseXI GCAGC 6 cut(s) 328, 331, 632, 643, 760, 809
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 1 cut(s) 639
BshFI GGCC 1 cut(s) 287
BshTI ACCGGT 1 cut(s) 314
BsiHKAI GWGCWC 5 cut(s) 102, 195, 555, 681, 1008
BsiSI CCGG 3 cut(s) 288, 315, 705
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 355, 502
BsmAI GTCTC 2 cut(s) 542, 955
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 542
Bsp1286I GDGCHC 6 cut(s) 102, 195, 555, 681, 726, 1008
Bsp143I GATC 6 cut(s) 21, 27, 115, 270, 789, 999
Bsp19I CCATGG 2 cut(s) 169, 275
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 808
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 260
BspMI ACCTGC 1 cut(s) 879
BspPI GGATC 2 cut(s) 110, 265
BspQI GCTCTTC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 542
BsrFI RCCGGY 2 cut(s) 287, 314
BsrI ACTGG 3 cut(s) 468, 608, 655
BssAI RCCGGY 2 cut(s) 287, 314
BssECI CCNNGG 6 cut(s) 169, 275, 501, 546, 660, 909
BssMI GATC 6 cut(s) 21, 27, 115, 270, 789, 999
BssT1I CCWWGG 5 cut(s) 169, 275, 501, 660, 909
Bst4CI ACNGT 7 cut(s) 166, 550, 580, 587, 730, 757, 980
Bst6I CTCTTC 2 cut(s) 84, 227
BstBAI YACGTR 1 cut(s) 1009
BstDEI CTNAG 1 cut(s) 816
BstDSI CCRYGG 3 cut(s) 169, 275, 546
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 1 cut(s) 403
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 6 cut(s) 24, 30, 118, 273, 792, 1002
BstMAI GTCTC 2 cut(s) 542, 955
BstMBI GATC 6 cut(s) 21, 27, 115, 270, 789, 999
BstMWI GCNNNNNNNGC 6 cut(s) 74, 190, 497, 662, 894, 920
BstNSI RCATGY 1 cut(s) 151
BstSLI GKGCMC 3 cut(s) 555, 726, 1008
BstV1I GCAGC 6 cut(s) 328, 331, 632, 643, 760, 809
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 1 cut(s) 287
BtgI CCRYGG 3 cut(s) 169, 275, 546
BtrI CACGTC 1 cut(s) 617
BtsCI GGATG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 561, 648, 726
BveI ACCTGC 1 cut(s) 879
CciI TCATGA 1 cut(s) 540
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 287, 314
Cfr13I GGNCC 1 cut(s) 587
Csp6I GTAC 2 cut(s) 157, 958
CspAI ACCGGT 1 cut(s) 314
CviAII CATG 9 cut(s) 139, 148, 170, 177, 188, 276, 406, 541, 954
CviQI GTAC 2 cut(s) 157, 958
DdeI CTNAG 1 cut(s) 816
DpnI GATC 6 cut(s) 23, 29, 117, 272, 791, 1001
DpnII GATC 6 cut(s) 21, 27, 115, 270, 789, 999
DrdI GACNNNNNNGTC 3 cut(s) 709, 755, 938
DriI GACNNNNNGTC 1 cut(s) 889
DseDI GACNNNNNNGTC 3 cut(s) 709, 755, 938
Eam1104I CTCTTC 2 cut(s) 84, 227
Eam1105I GACNNNNNGTC 1 cut(s) 889
EarI CTCTTC 2 cut(s) 84, 227
Ecl136II GAGCTC 2 cut(s) 100, 193
Eco130I CCWWGG 5 cut(s) 169, 275, 501, 660, 909
Eco24I GRGCYC 2 cut(s) 102, 195
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 2 cut(s) 100, 193
Eco57I CTGAAG 1 cut(s) 65
EcoICRI GAGCTC 2 cut(s) 100, 193
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoT14I CCWWGG 5 cut(s) 169, 275, 501, 660, 909
EcoT38I GRGCYC 2 cut(s) 102, 195
ErhI CCWWGG 5 cut(s) 169, 275, 501, 660, 909
FaeI CATG 9 cut(s) 142, 151, 173, 180, 191, 279, 409, 544, 957
FalI AAGNNNNNCTT 2 cut(s) 821, 853
FaqI GGGAC 1 cut(s) 101
FatI CATG 9 cut(s) 138, 147, 169, 176, 187, 275, 405, 540, 953
FblI GTMKAC 2 cut(s) 311, 993
Fnu4HI GCNGC 6 cut(s) 342, 345, 621, 657, 774, 798
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 2 cut(s) 102, 195
Fsp4HI GCNGC 6 cut(s) 342, 345, 621, 657, 774, 798
FspBI CTAG 3 cut(s) 69, 642, 1024
GlaI GCGC 1 cut(s) 175
GluI GCNGC 6 cut(s) 342, 345, 621, 657, 774, 798
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 2 cut(s) 625, 672
HaeIII GGCC 1 cut(s) 287
HapII CCGG 3 cut(s) 288, 315, 705
HhaI GCGC 1 cut(s) 176
Hin1II CATG 9 cut(s) 142, 151, 173, 180, 191, 279, 409, 544, 957
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 3 cut(s) 112, 583, 748
HindII GTYRAC 3 cut(s) 112, 583, 748
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 7 cut(s) 33, 51, 206, 252, 308, 812, 1018
HpaII CCGG 3 cut(s) 288, 315, 705
HphI GGTGA 5 cut(s) 118, 239, 293, 316, 893
Hpy188I TCNGA 6 cut(s) 32, 47, 56, 84, 251, 817
Hpy188III TCNNGA 3 cut(s) 25, 256, 541
HpyAV CCTTC 9 cut(s) 130, 227, 364, 424, 592, 637, 724, 763, 823
HpyCH4III ACNGT 7 cut(s) 166, 550, 580, 587, 730, 757, 980
HpyCH4IV ACGT 2 cut(s) 616, 1008
HpyCH4V TGCA 7 cut(s) 365, 474, 553, 620, 776, 923, 1006
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 190, 497, 662, 894, 920
HpyF3I CTNAG 1 cut(s) 816
HpySE526I ACGT 2 cut(s) 616, 1008
Hsp92II CATG 9 cut(s) 142, 151, 173, 180, 191, 279, 409, 544, 957
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 6 cut(s) 21, 27, 115, 270, 789, 999
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 5 cut(s) 391, 406, 496, 653, 684
Lsp1109I GCAGC 6 cut(s) 328, 331, 632, 643, 760, 809
LweI GCATC 4 cut(s) 388, 895, 906, 1022
MaeI CTAG 3 cut(s) 69, 642, 1024
MaeII ACGT 2 cut(s) 616, 1008
MaeIII GTNAC 3 cut(s) 842, 881, 974
MalI GATC 6 cut(s) 23, 29, 117, 272, 791, 1001
MboI GATC 6 cut(s) 21, 27, 115, 270, 789, 999
MboII GAAGA 5 cut(s) 31, 60, 71, 214, 863
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 6 cut(s) 102, 195, 555, 681, 726, 1008
MluCI AATT 3 cut(s) 525, 698, 918
MlyI GAGTC 3 cut(s) 200, 317, 821
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 8 cut(s) 78, 259, 277, 353, 526, 602, 802, 811
MseI TTAA 1 cut(s) 690
MspI CCGG 3 cut(s) 288, 315, 705
MwoI GCNNNNNNNGC 6 cut(s) 74, 190, 497, 662, 894, 920
NcoI CCATGG 2 cut(s) 169, 275
NdeII GATC 6 cut(s) 21, 27, 115, 270, 789, 999
NlaIII CATG 9 cut(s) 142, 151, 173, 180, 191, 279, 409, 544, 957
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 1 cut(s) 881
NspI RCATGY 1 cut(s) 151
PagI TCATGA 1 cut(s) 540
PciI ACATGT 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 867
PfeI GAWTC 3 cut(s) 33, 51, 252
PflFI GACNNNGTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 6 cut(s) 343, 346, 622, 658, 775, 799
PleI GAGTC 3 cut(s) 200, 316, 820
PpsI GAGTC 3 cut(s) 200, 316, 820
Ppu21I YACGTR 1 cut(s) 1009
PscI ACATGT 1 cut(s) 147
Psp124BI GAGCTC 2 cut(s) 102, 195
PspFI CCCAGC 1 cut(s) 389
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 587
PsuI RGATCY 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 2 cut(s) 158, 959
RsaNI GTAC 2 cut(s) 157, 958
SacI GAGCTC 2 cut(s) 102, 195
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 6 cut(s) 342, 345, 621, 657, 774, 798
Sau3AI GATC 6 cut(s) 21, 27, 115, 270, 789, 999
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 3 cut(s) 200, 317, 821
SduI GDGCHC 6 cut(s) 102, 195, 555, 681, 726, 1008
SfaNI GCATC 4 cut(s) 388, 895, 906, 1022
SinI GGWCC 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 194, 764
SmoI CTYRAG 2 cut(s) 194, 764
Sse9I AATT 3 cut(s) 525, 698, 918
SspMI CTAG 3 cut(s) 69, 642, 1024
SstI GAGCTC 2 cut(s) 102, 195
StyI CCWWGG 5 cut(s) 169, 275, 501, 660, 909
TaaI ACNGT 7 cut(s) 166, 550, 580, 587, 730, 757, 980
TaiI ACGT 2 cut(s) 619, 1011
TaqI TCGA 1 cut(s) 1016
TasI AATT 3 cut(s) 525, 698, 918
TfiI GAWTC 3 cut(s) 33, 51, 252
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 3 cut(s) 568, 655, 733
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 6 cut(s) 341, 344, 620, 656, 773, 797
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 4 cut(s) 112, 611, 798, 865
TspGWI ACGGA 2 cut(s) 707, 730
TspRI CASTG 3 cut(s) 568, 655, 733
Tth111I GACNNNGTC 1 cut(s) 164
VneI GTGCAC 2 cut(s) 551, 1004
VpaK11BI GGWCC 1 cut(s) 587
XagI CCTNNNNNAGG 1 cut(s) 500
XceI RCATGY 1 cut(s) 151
XmiI GTMKAC 2 cut(s) 311, 993
XspI CTAG 3 cut(s) 69, 642, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.