Belongs to the glyceraldehyde-3-phosphate dehydrogenase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 598 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 808 |
| AccI | GTMKAC | 1 cut(s) | 636 |
| AciI | CCGC | 1 cut(s) | 213 |
| AclI | AACGTT | 1 cut(s) | 1008 |
| AclWI | GGATC | 1 cut(s) | 900 |
| AcsI | RAATTY | 2 cut(s) | 188, 371 |
| AcuI | CTGAAG | 1 cut(s) | 732 |
| AfaI | GTAC | 4 cut(s) | 172, 400, 821, 904 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 134, 208, 248, 355, 808 |
| AflII | CTTAAG | 1 cut(s) | 53 |
| AflIII | ACRYGT | 1 cut(s) | 443 |
| AgsI | TTSAA | 2 cut(s) | 208, 790 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 751 |
| AjnI | CCWGG | 3 cut(s) | 463, 576, 914 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 683, 715 |
| AluBI | AGCT | 6 cut(s) | 301, 473, 509, 545, 665, 851 |
| AluI | AGCT | 6 cut(s) | 301, 473, 509, 545, 665, 851 |
| Alw21I | GWGCWC | 1 cut(s) | 256 |
| Alw26I | GTCTC | 3 cut(s) | 454, 643, 730 |
| AlwI | GGATC | 1 cut(s) | 900 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 416 |
| AoxI | GGCC | 4 cut(s) | 136, 199, 774, 912 |
| ApeKI | GCWGC | 3 cut(s) | 473, 509, 545 |
| ApoI | RAATTY | 2 cut(s) | 188, 371 |
| AspS9I | GGNCC | 2 cut(s) | 137, 199 |
| AsuC2I | CCSGG | 1 cut(s) | 141 |
| AsuHPI | GGTGA | 2 cut(s) | 85, 755 |
| AsuNHI | GCTAGC | 1 cut(s) | 419 |
| BbsI | GAAGAC | 1 cut(s) | 123 |
| Bbv12I | GWGCWC | 1 cut(s) | 256 |
| BbvI | GCAGC | 3 cut(s) | 460, 496, 532 |
| BccI | CCATC | 2 cut(s) | 431, 575 |
| BciT130I | CCWGG | 3 cut(s) | 465, 578, 916 |
| BciVI | GTATCC | 1 cut(s) | 830 |
| BcnI | CCSGG | 1 cut(s) | 141 |
| BcoDI | GTCTC | 3 cut(s) | 454, 643, 730 |
| BfaI | CTAG | 4 cut(s) | 321, 420, 615, 653 |
| BfrI | CTTAAG | 1 cut(s) | 53 |
| BfuI | GTATCC | 1 cut(s) | 830 |
| BisI | GCNGC | 3 cut(s) | 474, 510, 546 |
| BlsI | GCNGC | 3 cut(s) | 475, 511, 547 |
| BmcAI | AGTACT | 1 cut(s) | 400 |
| Bme1390I | CCNGG | 4 cut(s) | 141, 465, 578, 916 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 751 |
| BmgT120I | GGNCC | 2 cut(s) | 137, 199 |
| BmiI | GGNNCC | 2 cut(s) | 139, 145 |
| BmrFI | CCNGG | 4 cut(s) | 141, 465, 578, 916 |
| BmsI | GCATC | 2 cut(s) | 151, 757 |
| BmtI | GCTAGC | 1 cut(s) | 423 |
| BpiI | GAAGAC | 1 cut(s) | 123 |
| Bpu10I | CCTNAGC | 2 cut(s) | 195, 549 |
| BpuMI | CCSGG | 1 cut(s) | 141 |
| Bsa29I | ATCGAT | 1 cut(s) | 895 |
| BsaAI | YACGTR | 1 cut(s) | 902 |
| BsaI | GGTCTC | 2 cut(s) | 454, 730 |
| BsaJI | CCNNGG | 3 cut(s) | 22, 128, 139 |
| BsaWI | WCCGGW | 4 cut(s) | 314, 539, 593, 876 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 134, 208, 248, 355, 808 |
| Bse1I | ACTGG | 1 cut(s) | 894 |
| BseBI | CCWGG | 3 cut(s) | 465, 578, 916 |
| BseCI | ATCGAT | 1 cut(s) | 895 |
| BseDI | CCNNGG | 3 cut(s) | 22, 128, 139 |
| BseGI | GGATG | 2 cut(s) | 166, 586 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 134, 208, 248, 355, 808 |
| BseMII | CTCAG | 3 cut(s) | 209, 423, 669 |
| BseNI | ACTGG | 1 cut(s) | 894 |
| BseXI | GCAGC | 3 cut(s) | 460, 496, 532 |
| BsgI | GTGCAG | 1 cut(s) | 459 |
| Bsh1285I | CGRYCG | 1 cut(s) | 881 |
| BshFI | GGCC | 4 cut(s) | 138, 201, 776, 914 |
| BshVI | ATCGAT | 1 cut(s) | 895 |
| BsiEI | CGRYCG | 1 cut(s) | 881 |
| BsiHKAI | GWGCWC | 1 cut(s) | 256 |
| BsiSI | CCGG | 7 cut(s) | 141, 296, 315, 540, 594, 777, 877 |
| BsiWI | CGTACG | 1 cut(s) | 902 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 134, 208, 248, 355, 808 |
| BsmAI | GTCTC | 3 cut(s) | 454, 643, 730 |
| BsmI | GAATGC | 1 cut(s) | 64 |
| BsnI | GGCC | 4 cut(s) | 138, 201, 776, 914 |
| Bso31I | GGTCTC | 2 cut(s) | 454, 730 |
| Bsp1286I | GDGCHC | 1 cut(s) | 256 |
| Bsp143I | GATC | 2 cut(s) | 892, 896 |
| BspACI | CCGC | 1 cut(s) | 213 |
| BspANI | GGCC | 4 cut(s) | 138, 201, 776, 914 |
| BspCNI | CTCAG | 3 cut(s) | 208, 422, 670 |
| BspDI | ATCGAT | 1 cut(s) | 895 |
| BspHI | TCATGA | 1 cut(s) | 364 |
| BspLI | GGNNCC | 2 cut(s) | 139, 145 |
| BspOI | GCTAGC | 1 cut(s) | 423 |
| BspPI | GGATC | 1 cut(s) | 900 |
| BspQI | GCTCTTC | 1 cut(s) | 499 |
| BspTI | CTTAAG | 1 cut(s) | 53 |
| BspTNI | GGTCTC | 2 cut(s) | 454, 730 |
| BsrI | ACTGG | 1 cut(s) | 894 |
| BssECI | CCNNGG | 3 cut(s) | 22, 128, 139 |
| BssMI | GATC | 2 cut(s) | 892, 896 |
| BssT1I | CCWWGG | 1 cut(s) | 128 |
| Bst2UI | CCWGG | 3 cut(s) | 465, 578, 916 |
| Bst4CI | ACNGT | 6 cut(s) | 19, 26, 251, 403, 646, 842 |
| Bst6I | CTCTTC | 3 cut(s) | 499, 666, 958 |
| BstAFI | CTTAAG | 1 cut(s) | 53 |
| BstBAI | YACGTR | 1 cut(s) | 902 |
| BstC8I | GCNNGC | 2 cut(s) | 421, 663 |
| BstDEI | CTNAG | 4 cut(s) | 195, 409, 549, 678 |
| BstDSI | CCRYGG | 1 cut(s) | 22 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 353 |
| BstF5I | GGATG | 2 cut(s) | 166, 586 |
| BstKTI | GATC | 2 cut(s) | 895, 899 |
| BstMAI | GTCTC | 3 cut(s) | 454, 643, 730 |
| BstMBI | GATC | 2 cut(s) | 892, 896 |
| BstMCI | CGRYCG | 1 cut(s) | 881 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 350, 551, 782 |
| BstNI | CCWGG | 3 cut(s) | 465, 578, 916 |
| BstNSI | RCATGY | 1 cut(s) | 447 |
| BstSCI | CCNGG | 4 cut(s) | 139, 463, 576, 914 |
| BstV1I | GCAGC | 3 cut(s) | 460, 496, 532 |
| BstV2I | GAAGAC | 1 cut(s) | 123 |
| Bsu15I | ATCGAT | 1 cut(s) | 895 |
| BsuI | GTATCC | 1 cut(s) | 830 |
| BsuRI | GGCC | 4 cut(s) | 138, 201, 776, 914 |
| BsuTUI | ATCGAT | 1 cut(s) | 895 |
| BtgI | CCRYGG | 1 cut(s) | 22 |
| BtgZI | GCGATG | 1 cut(s) | 57 |
| BtsCI | GGATG | 2 cut(s) | 166, 586 |
| BtsIMutI | CAGTG | 4 cut(s) | 15, 651, 708, 1004 |
| Cac8I | GCNNGC | 2 cut(s) | 421, 663 |
| CaiI | CAGNNNCTG | 1 cut(s) | 416 |
| CciI | TCATGA | 1 cut(s) | 364 |
| Cfr13I | GGNCC | 2 cut(s) | 137, 199 |
| ClaI | ATCGAT | 1 cut(s) | 895 |
| Csp6I | GTAC | 4 cut(s) | 171, 399, 820, 903 |
| CviAII | CATG | 4 cut(s) | 30, 365, 444, 816 |
| CviQI | GTAC | 4 cut(s) | 171, 399, 820, 903 |
| DdeI | CTNAG | 4 cut(s) | 195, 409, 549, 678 |
| DpnI | GATC | 2 cut(s) | 894, 898 |
| DpnII | GATC | 2 cut(s) | 892, 896 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 598 |
| DriI | GACNNNNNGTC | 1 cut(s) | 751 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 598 |
| Eam1104I | CTCTTC | 3 cut(s) | 499, 666, 958 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 751 |
| EarI | CTCTTC | 3 cut(s) | 499, 666, 958 |
| EciI | GGCGGA | 1 cut(s) | 228 |
| Eco130I | CCWWGG | 1 cut(s) | 128 |
| Eco31I | GGTCTC | 2 cut(s) | 454, 730 |
| Eco57I | CTGAAG | 1 cut(s) | 732 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 353 |
| EcoRII | CCWGG | 3 cut(s) | 463, 576, 914 |
| EcoT14I | CCWWGG | 1 cut(s) | 128 |
| ErhI | CCWWGG | 1 cut(s) | 128 |
| FaeI | CATG | 4 cut(s) | 33, 368, 447, 819 |
| FaiI | YATR | 5 cut(s) | 31, 366, 445, 516, 817 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 496, 528 |
| FatI | CATG | 4 cut(s) | 29, 364, 443, 815 |
| FblI | GTMKAC | 1 cut(s) | 636 |
| Fnu4HI | GCNGC | 3 cut(s) | 474, 510, 546 |
| FokI | GGATG | 2 cut(s) | 173, 593 |
| Fsp4HI | GCNGC | 3 cut(s) | 474, 510, 546 |
| FspBI | CTAG | 4 cut(s) | 321, 420, 615, 653 |
| GluI | GCNGC | 3 cut(s) | 474, 510, 546 |
| HaeIII | GGCC | 4 cut(s) | 138, 201, 776, 914 |
| HapII | CCGG | 7 cut(s) | 141, 296, 315, 540, 594, 777, 877 |
| Hin1II | CATG | 4 cut(s) | 33, 368, 447, 819 |
| HincII | GTYRAC | 1 cut(s) | 861 |
| HindII | GTYRAC | 1 cut(s) | 861 |
| HinfI | GANTC | 2 cut(s) | 674, 696 |
| HpaI | GTTAAC | 1 cut(s) | 861 |
| HpaII | CCGG | 7 cut(s) | 141, 296, 315, 540, 594, 777, 877 |
| HphI | GGTGA | 2 cut(s) | 85, 755 |
| Hpy166II | GTNNAC | 6 cut(s) | 6, 22, 73, 406, 637, 861 |
| Hpy188I | TCNGA | 3 cut(s) | 393, 679, 855 |
| Hpy188III | TCNNGA | 2 cut(s) | 365, 620 |
| Hpy8I | GTNNAC | 6 cut(s) | 6, 22, 73, 406, 637, 861 |
| Hpy99I | CGWCG | 1 cut(s) | 859 |
| HpyAV | CCTTC | 4 cut(s) | 202, 217, 277, 901 |
| HpyCH4III | ACNGT | 6 cut(s) | 19, 26, 251, 403, 646, 842 |
| HpyCH4IV | ACGT | 5 cut(s) | 69, 260, 857, 901, 1008 |
| HpyCH4V | TGCA | 9 cut(s) | 29, 62, 104, 327, 353, 476, 770, 815, 1004 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 350, 551, 782 |
| HpyF3I | CTNAG | 4 cut(s) | 195, 409, 549, 678 |
| HpySE526I | ACGT | 5 cut(s) | 69, 260, 857, 901, 1008 |
| Hsp92II | CATG | 4 cut(s) | 33, 368, 447, 819 |
| KspAI | GTTAAC | 1 cut(s) | 861 |
| Kzo9I | GATC | 2 cut(s) | 892, 896 |
| LguI | GCTCTTC | 1 cut(s) | 499 |
| LmnI | GCTCC | 6 cut(s) | 143, 215, 259, 298, 542, 856 |
| Lsp1109I | GCAGC | 3 cut(s) | 460, 496, 532 |
| LweI | GCATC | 2 cut(s) | 151, 757 |
| MaeI | CTAG | 4 cut(s) | 321, 420, 615, 653 |
| MaeII | ACGT | 5 cut(s) | 69, 260, 857, 901, 1008 |
| MaeIII | GTNAC | 7 cut(s) | 80, 91, 232, 245, 412, 704, 743 |
| MalI | GATC | 2 cut(s) | 894, 898 |
| MboI | GATC | 2 cut(s) | 892, 896 |
| MboII | GAAGA | 7 cut(s) | 46, 128, 516, 683, 725, 975, 993 |
| MhlI | GDGCHC | 1 cut(s) | 256 |
| MluCI | AATT | 7 cut(s) | 37, 188, 360, 371, 498, 587, 780 |
| MlyI | GAGTC | 1 cut(s) | 683 |
| MmeI | TCCRAC | 1 cut(s) | 878 |
| MnlI | CCTC | 6 cut(s) | 175, 379, 422, 673, 733, 959 |
| MseI | TTAA | 3 cut(s) | 54, 303, 860 |
| MspCI | CTTAAG | 1 cut(s) | 53 |
| MspI | CCGG | 7 cut(s) | 141, 296, 315, 540, 594, 777, 877 |
| MspR9I | CCNGG | 4 cut(s) | 141, 465, 578, 916 |
| Mva1269I | GAATGC | 1 cut(s) | 64 |
| MvaI | CCWGG | 3 cut(s) | 465, 578, 916 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 350, 551, 782 |
| NciI | CCSGG | 1 cut(s) | 141 |
| NdeII | GATC | 2 cut(s) | 892, 896 |
| NheI | GCTAGC | 1 cut(s) | 419 |
| NlaIII | CATG | 4 cut(s) | 33, 368, 447, 819 |
| NlaIV | GGNNCC | 2 cut(s) | 139, 145 |
| NmeAIII | GCCGAG | 1 cut(s) | 137 |
| NmuCI | GTSAC | 4 cut(s) | 91, 232, 245, 743 |
| NspI | RCATGY | 1 cut(s) | 447 |
| PagI | TCATGA | 1 cut(s) | 364 |
| PciI | ACATGT | 1 cut(s) | 443 |
| PciSI | GCTCTTC | 1 cut(s) | 499 |
| PctI | GAATGC | 1 cut(s) | 64 |
| PfeI | GAWTC | 1 cut(s) | 696 |
| Pfl23II | CGTACG | 1 cut(s) | 902 |
| PflFI | GACNNNGTC | 1 cut(s) | 17 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 808 |
| PkrI | GCNGC | 3 cut(s) | 475, 511, 547 |
| PleI | GAGTC | 1 cut(s) | 682 |
| PpsI | GAGTC | 1 cut(s) | 682 |
| Ppu21I | YACGTR | 1 cut(s) | 902 |
| PscI | ACATGT | 1 cut(s) | 443 |
| Psp1406I | AACGTT | 1 cut(s) | 1008 |
| Psp6I | CCWGG | 3 cut(s) | 463, 576, 914 |
| PspGI | CCWGG | 3 cut(s) | 463, 576, 914 |
| PspLI | CGTACG | 1 cut(s) | 902 |
| PspN4I | GGNNCC | 2 cut(s) | 139, 145 |
| PspPI | GGNCC | 2 cut(s) | 137, 199 |
| PstNI | CAGNNNCTG | 1 cut(s) | 416 |
| PsyI | GACNNNGTC | 1 cut(s) | 17 |
| RsaI | GTAC | 4 cut(s) | 172, 400, 821, 904 |
| RsaNI | GTAC | 4 cut(s) | 171, 399, 820, 903 |
| SapI | GCTCTTC | 1 cut(s) | 499 |
| SaqAI | TTAA | 3 cut(s) | 54, 303, 860 |
| SatI | GCNGC | 3 cut(s) | 474, 510, 546 |
| Sau3AI | GATC | 2 cut(s) | 892, 896 |
| Sau96I | GGNCC | 2 cut(s) | 137, 199 |
| ScaI | AGTACT | 1 cut(s) | 400 |
| SchI | GAGTC | 1 cut(s) | 683 |
| ScrFI | CCNGG | 4 cut(s) | 141, 465, 578, 916 |
| SduI | GDGCHC | 1 cut(s) | 256 |
| SfaNI | GCATC | 2 cut(s) | 151, 757 |
| SmlI | CTYRAG | 1 cut(s) | 53 |
| SmoI | CTYRAG | 1 cut(s) | 53 |
| Sse9I | AATT | 7 cut(s) | 37, 188, 360, 371, 498, 587, 780 |
| SsiI | CCGC | 1 cut(s) | 213 |
| SspMI | CTAG | 4 cut(s) | 321, 420, 615, 653 |
| StyD4I | CCNGG | 4 cut(s) | 139, 463, 576, 914 |
| StyI | CCWWGG | 1 cut(s) | 128 |
| TaaI | ACNGT | 6 cut(s) | 19, 26, 251, 403, 646, 842 |
| TaiI | ACGT | 5 cut(s) | 72, 263, 860, 904, 1011 |
| TaqI | TCGA | 4 cut(s) | 186, 384, 881, 895 |
| TasI | AATT | 7 cut(s) | 37, 188, 360, 371, 498, 587, 780 |
| TatI | WGTACW | 1 cut(s) | 398 |
| TfiI | GAWTC | 1 cut(s) | 696 |
| Tru1I | TTAA | 3 cut(s) | 54, 303, 860 |
| Tru9I | TTAA | 3 cut(s) | 54, 303, 860 |
| TscAI | CASTG | 4 cut(s) | 22, 651, 715, 1004 |
| TseFI | GTSAC | 4 cut(s) | 91, 232, 245, 743 |
| TseI | GCWGC | 3 cut(s) | 473, 509, 545 |
| Tsp45I | GTSAC | 4 cut(s) | 91, 232, 245, 743 |
| TspDTI | ATGAA | 1 cut(s) | 353 |
| TspGWI | ACGGA | 2 cut(s) | 596, 683 |
| TspRI | CASTG | 4 cut(s) | 22, 651, 715, 1004 |
| Tth111I | GACNNNGTC | 1 cut(s) | 17 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 808 |
| Vha464I | CTTAAG | 1 cut(s) | 53 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 353 |
| XapI | RAATTY | 2 cut(s) | 188, 371 |
| XceI | RCATGY | 1 cut(s) | 447 |
| XmiI | GTMKAC | 1 cut(s) | 636 |
| XspI | CTAG | 4 cut(s) | 321, 420, 615, 653 |
| ZrmI | AGTACT | 1 cut(s) | 400 |