Belongs to the glyceraldehyde-3-phosphate dehydrogenase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 599 |
| AciI | CCGC | 1 cut(s) | 46 |
| AclWI | GGATC | 4 cut(s) | 98, 245, 258, 327 |
| AcoI | YGGCCR | 2 cut(s) | 3, 43 |
| AcsI | RAATTY | 4 cut(s) | 234, 287, 509, 1039 |
| AfaI | GTAC | 3 cut(s) | 146, 260, 428 |
| AflII | CTTAAG | 1 cut(s) | 191 |
| AflIII | ACRYGT | 1 cut(s) | 135 |
| AgsI | TTSAA | 2 cut(s) | 563, 922 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 883 |
| AluBI | AGCT | 7 cut(s) | 332, 377, 462, 611, 636, 694, 975 |
| AluI | AGCT | 7 cut(s) | 332, 377, 462, 611, 636, 694, 975 |
| Alw26I | GTCTC | 2 cut(s) | 748, 949 |
| AlwI | GGATC | 4 cut(s) | 98, 245, 258, 327 |
| AoxI | GGCC | 5 cut(s) | 3, 43, 300, 705, 1005 |
| ApeKI | GCWGC | 3 cut(s) | 332, 611, 673 |
| ApoI | RAATTY | 4 cut(s) | 234, 287, 509, 1039 |
| AspLEI | GCGC | 1 cut(s) | 621 |
| AspS9I | GGNCC | 1 cut(s) | 541 |
| AsuC2I | CCSGG | 1 cut(s) | 227 |
| AsuHPI | GGTGA | 3 cut(s) | 64, 230, 887 |
| AvaII | GGWCC | 1 cut(s) | 541 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BamHI | GGATCC | 1 cut(s) | 250 |
| BauI | CACGAG | 1 cut(s) | 978 |
| BbsI | GAAGAC | 1 cut(s) | 209 |
| BbvI | GCAGC | 3 cut(s) | 319, 598, 660 |
| BccI | CCATC | 1 cut(s) | 650 |
| BceAI | ACGGC | 1 cut(s) | 554 |
| BciVI | GTATCC | 1 cut(s) | 830 |
| BcnI | CCSGG | 1 cut(s) | 227 |
| BcoDI | GTCTC | 2 cut(s) | 748, 949 |
| BfaI | CTAG | 2 cut(s) | 633, 648 |
| BfrI | CTTAAG | 1 cut(s) | 191 |
| BfuI | GTATCC | 1 cut(s) | 830 |
| BglII | AGATCT | 1 cut(s) | 436 |
| BisI | GCNGC | 4 cut(s) | 46, 333, 612, 674 |
| BlsI | GCNGC | 4 cut(s) | 47, 334, 613, 675 |
| Bme1390I | CCNGG | 1 cut(s) | 227 |
| Bme18I | GGWCC | 1 cut(s) | 541 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 883 |
| BmgT120I | GGNCC | 1 cut(s) | 541 |
| BmiI | GGNNCC | 1 cut(s) | 252 |
| BmrFI | CCNGG | 1 cut(s) | 227 |
| BmsI | GCATC | 5 cut(s) | 106, 271, 379, 565, 889 |
| BoxI | GACNNNNGTC | 1 cut(s) | 539 |
| BpiI | GAAGAC | 1 cut(s) | 209 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 321, 353 |
| BpmI | CTGGAG | 2 cut(s) | 417, 616 |
| BpuEI | CTTGAG | 1 cut(s) | 779 |
| BpuMI | CCSGG | 1 cut(s) | 227 |
| Bsa29I | ATCGAT | 1 cut(s) | 114 |
| BsaJI | CCNNGG | 3 cut(s) | 266, 832, 903 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 589, 619 |
| Bse118I | RCCGGY | 1 cut(s) | 676 |
| Bse1I | ACTGG | 2 cut(s) | 599, 687 |
| BseCI | ATCGAT | 1 cut(s) | 114 |
| BseDI | CCNNGG | 3 cut(s) | 266, 832, 903 |
| BseGI | GGATG | 1 cut(s) | 261 |
| BseMII | CTCAG | 1 cut(s) | 801 |
| BseNI | ACTGG | 2 cut(s) | 599, 687 |
| BseX3I | CGGCCG | 1 cut(s) | 43 |
| BseXI | GCAGC | 3 cut(s) | 319, 598, 660 |
| BseYI | CCCAGC | 1 cut(s) | 614 |
| Bsh1285I | CGRYCG | 1 cut(s) | 46 |
| BshFI | GGCC | 5 cut(s) | 5, 45, 302, 707, 1007 |
| BshVI | ATCGAT | 1 cut(s) | 114 |
| BsiEI | CGRYCG | 1 cut(s) | 46 |
| BsiSI | CCGG | 2 cut(s) | 227, 677 |
| BsmAI | GTCTC | 2 cut(s) | 748, 949 |
| BsmI | GAATGC | 1 cut(s) | 714 |
| BsnI | GGCC | 5 cut(s) | 5, 45, 302, 707, 1007 |
| Bsp1407I | TGTACA | 1 cut(s) | 258 |
| Bsp143I | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| Bsp19I | CCATGG | 1 cut(s) | 266 |
| BspACI | CCGC | 1 cut(s) | 46 |
| BspANI | GGCC | 5 cut(s) | 5, 45, 302, 707, 1007 |
| BspCNI | CTCAG | 1 cut(s) | 802 |
| BspDI | ATCGAT | 1 cut(s) | 114 |
| BspLI | GGNNCC | 1 cut(s) | 252 |
| BspPI | GGATC | 4 cut(s) | 98, 245, 258, 327 |
| BspTI | CTTAAG | 1 cut(s) | 191 |
| BsrFI | RCCGGY | 1 cut(s) | 676 |
| BsrGI | TGTACA | 1 cut(s) | 258 |
| BsrI | ACTGG | 2 cut(s) | 599, 687 |
| BssAI | RCCGGY | 1 cut(s) | 676 |
| BssECI | CCNNGG | 3 cut(s) | 266, 832, 903 |
| BssMI | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| BssSI | CACGAG | 1 cut(s) | 978 |
| BssT1I | CCWWGG | 3 cut(s) | 266, 832, 903 |
| Bst2BI | CACGAG | 1 cut(s) | 978 |
| Bst4CI | ACNGT | 5 cut(s) | 161, 476, 541, 724, 751 |
| BstAFI | CTTAAG | 1 cut(s) | 191 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 464 |
| BstAUI | TGTACA | 1 cut(s) | 258 |
| BstC8I | GCNNGC | 3 cut(s) | 460, 678, 768 |
| BstDEI | CTNAG | 1 cut(s) | 810 |
| BstDSI | CCRYGG | 1 cut(s) | 266 |
| BstEII | GGTNACC | 1 cut(s) | 218 |
| BstF5I | GGATG | 1 cut(s) | 261 |
| BstHHI | GCGC | 1 cut(s) | 621 |
| BstKTI | GATC | 8 cut(s) | 12, 106, 132, 253, 322, 439, 786, 996 |
| BstMAI | GTCTC | 2 cut(s) | 748, 949 |
| BstMBI | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| BstMCI | CGRYCG | 1 cut(s) | 46 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 332, 464, 488, 575, 914 |
| BstNSI | RCATGY | 1 cut(s) | 139 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 539 |
| BstPI | GGTNACC | 1 cut(s) | 218 |
| BstSCI | CCNGG | 1 cut(s) | 225 |
| BstV1I | GCAGC | 3 cut(s) | 319, 598, 660 |
| BstV2I | GAAGAC | 1 cut(s) | 209 |
| BstX2I | RGATCY | 2 cut(s) | 250, 436 |
| BstYI | RGATCY | 2 cut(s) | 250, 436 |
| BstZI | CGGCCG | 1 cut(s) | 43 |
| Bsu15I | ATCGAT | 1 cut(s) | 114 |
| BsuI | GTATCC | 1 cut(s) | 830 |
| BsuRI | GGCC | 5 cut(s) | 5, 45, 302, 707, 1007 |
| BsuTUI | ATCGAT | 1 cut(s) | 114 |
| BtgI | CCRYGG | 1 cut(s) | 266 |
| BtgZI | GCGATG | 1 cut(s) | 402 |
| BtsCI | GGATG | 1 cut(s) | 261 |
| BtsIMutI | CAGTG | 4 cut(s) | 680, 720, 747, 840 |
| Cac8I | GCNNGC | 3 cut(s) | 460, 678, 768 |
| CfoI | GCGC | 1 cut(s) | 621 |
| Cfr10I | RCCGGY | 1 cut(s) | 676 |
| Cfr13I | GGNCC | 1 cut(s) | 541 |
| ClaI | ATCGAT | 1 cut(s) | 114 |
| Csp6I | GTAC | 3 cut(s) | 145, 259, 427 |
| CviAII | CATG | 7 cut(s) | 136, 179, 267, 323, 503, 582, 1003 |
| CviQI | GTAC | 3 cut(s) | 145, 259, 427 |
| DdeI | CTNAG | 1 cut(s) | 810 |
| DpnI | GATC | 8 cut(s) | 11, 105, 131, 252, 321, 438, 785, 995 |
| DpnII | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 599 |
| DriI | GACNNNNNGTC | 1 cut(s) | 883 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 599 |
| EaeI | YGGCCR | 2 cut(s) | 3, 43 |
| EagI | CGGCCG | 1 cut(s) | 43 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 883 |
| EclXI | CGGCCG | 1 cut(s) | 43 |
| Eco130I | CCWWGG | 3 cut(s) | 266, 832, 903 |
| Eco147I | AGGCCT | 1 cut(s) | 302 |
| Eco47I | GGWCC | 1 cut(s) | 541 |
| Eco52I | CGGCCG | 1 cut(s) | 43 |
| Eco91I | GGTNACC | 1 cut(s) | 218 |
| EcoO65I | GGTNACC | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 3 cut(s) | 266, 832, 903 |
| ErhI | CCWWGG | 3 cut(s) | 266, 832, 903 |
| FaeI | CATG | 7 cut(s) | 139, 182, 270, 326, 506, 585, 1006 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 816, 848 |
| FatI | CATG | 7 cut(s) | 135, 178, 266, 322, 502, 581, 1002 |
| FauNDI | CATATG | 1 cut(s) | 342 |
| Fnu4HI | GCNGC | 4 cut(s) | 46, 333, 612, 674 |
| FokI | GGATG | 1 cut(s) | 248 |
| Fsp4HI | GCNGC | 4 cut(s) | 46, 333, 612, 674 |
| FspBI | CTAG | 2 cut(s) | 633, 648 |
| GlaI | GCGC | 1 cut(s) | 620 |
| GluI | GCNGC | 4 cut(s) | 46, 333, 612, 674 |
| GsaI | CCCAGC | 1 cut(s) | 618 |
| GsuI | CTGGAG | 2 cut(s) | 417, 616 |
| HaeIII | GGCC | 5 cut(s) | 5, 45, 302, 707, 1007 |
| HapII | CCGG | 2 cut(s) | 227, 677 |
| HhaI | GCGC | 1 cut(s) | 621 |
| Hin1II | CATG | 7 cut(s) | 139, 182, 270, 326, 506, 585, 1006 |
| Hin6I | GCGC | 1 cut(s) | 619 |
| HinP1I | GCGC | 1 cut(s) | 619 |
| HincII | GTYRAC | 1 cut(s) | 742 |
| HindII | GTYRAC | 1 cut(s) | 742 |
| HinfI | GANTC | 4 cut(s) | 39, 600, 806, 828 |
| HpaII | CCGG | 2 cut(s) | 227, 677 |
| HphI | GGTGA | 3 cut(s) | 64, 230, 887 |
| Hpy166II | GTNNAC | 5 cut(s) | 157, 259, 429, 544, 742 |
| Hpy188I | TCNGA | 4 cut(s) | 515, 588, 811, 1033 |
| Hpy188III | TCNNGA | 6 cut(s) | 13, 306, 434, 799, 978, 1044 |
| Hpy8I | GTNNAC | 5 cut(s) | 157, 259, 429, 544, 742 |
| Hpy99I | CGWCG | 1 cut(s) | 575 |
| HpyAV | CCTTC | 4 cut(s) | 355, 415, 680, 718 |
| HpyCH4III | ACNGT | 5 cut(s) | 161, 476, 541, 724, 751 |
| HpyCH4V | TGCA | 5 cut(s) | 346, 356, 458, 578, 714 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 332, 464, 488, 575, 914 |
| HpyF3I | CTNAG | 1 cut(s) | 810 |
| Hsp92II | CATG | 7 cut(s) | 139, 182, 270, 326, 506, 585, 1006 |
| HspAI | GCGC | 1 cut(s) | 619 |
| KroI | GCCGGC | 1 cut(s) | 676 |
| KroNI | GCCGGC | 1 cut(s) | 678 |
| Kzo9I | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| LmnI | GCTCC | 4 cut(s) | 382, 397, 487, 641 |
| Lsp1109I | GCAGC | 3 cut(s) | 319, 598, 660 |
| LweI | GCATC | 5 cut(s) | 106, 271, 379, 565, 889 |
| MaeI | CTAG | 2 cut(s) | 633, 648 |
| MaeIII | GTNAC | 4 cut(s) | 52, 218, 875, 998 |
| MalI | GATC | 8 cut(s) | 11, 105, 131, 252, 321, 438, 785, 995 |
| MboI | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| MboII | GAAGA | 2 cut(s) | 48, 214 |
| MflI | RGATCY | 2 cut(s) | 250, 436 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 7 cut(s) | 234, 287, 369, 509, 516, 912, 1039 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 609 |
| MnlI | CCTC | 8 cut(s) | 268, 292, 313, 517, 755, 805, 1018, 1027 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MroNI | GCCGGC | 1 cut(s) | 676 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 3 cut(s) | 141, 192, 441 |
| MslI | CAYNNNNRTG | 1 cut(s) | 177 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspCI | CTTAAG | 1 cut(s) | 191 |
| MspI | CCGG | 2 cut(s) | 227, 677 |
| MspR9I | CCNGG | 1 cut(s) | 227 |
| Mva1269I | GAATGC | 1 cut(s) | 714 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 332, 464, 488, 575, 914 |
| NaeI | GCCGGC | 1 cut(s) | 678 |
| NciI | CCSGG | 1 cut(s) | 227 |
| NcoI | CCATGG | 1 cut(s) | 266 |
| NdeI | CATATG | 1 cut(s) | 342 |
| NdeII | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| NgoMIV | GCCGGC | 1 cut(s) | 676 |
| NlaIII | CATG | 7 cut(s) | 139, 182, 270, 326, 506, 585, 1006 |
| NlaIV | GGNNCC | 1 cut(s) | 252 |
| NmuCI | GTSAC | 4 cut(s) | 52, 218, 875, 998 |
| NspI | RCATGY | 1 cut(s) | 139 |
| PceI | AGGCCT | 1 cut(s) | 302 |
| PciI | ACATGT | 1 cut(s) | 135 |
| PctI | GAATGC | 1 cut(s) | 714 |
| PdiI | GCCGGC | 1 cut(s) | 678 |
| PfeI | GAWTC | 3 cut(s) | 39, 806, 828 |
| PkrI | GCNGC | 4 cut(s) | 47, 334, 613, 675 |
| PleI | GAGTC | 1 cut(s) | 608 |
| PpsI | GAGTC | 1 cut(s) | 608 |
| PscI | ACATGT | 1 cut(s) | 135 |
| PshAI | GACNNNNGTC | 1 cut(s) | 539 |
| PspEI | GGTNACC | 1 cut(s) | 218 |
| PspFI | CCCAGC | 1 cut(s) | 614 |
| PspN4I | GGNNCC | 1 cut(s) | 252 |
| PspPI | GGNCC | 1 cut(s) | 541 |
| PsuI | RGATCY | 2 cut(s) | 250, 436 |
| RsaI | GTAC | 3 cut(s) | 146, 260, 428 |
| RsaNI | GTAC | 3 cut(s) | 145, 259, 427 |
| RseI | CAYNNNNRTG | 1 cut(s) | 177 |
| SaqAI | TTAA | 3 cut(s) | 141, 192, 441 |
| SatI | GCNGC | 4 cut(s) | 46, 333, 612, 674 |
| Sau3AI | GATC | 8 cut(s) | 9, 103, 129, 250, 319, 436, 783, 993 |
| Sau96I | GGNCC | 1 cut(s) | 541 |
| SchI | GAGTC | 1 cut(s) | 609 |
| ScrFI | CCNGG | 1 cut(s) | 227 |
| SfaNI | GCATC | 5 cut(s) | 106, 271, 379, 565, 889 |
| SinI | GGWCC | 1 cut(s) | 541 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 177 |
| SmlI | CTYRAG | 2 cut(s) | 191, 758 |
| SmoI | CTYRAG | 2 cut(s) | 191, 758 |
| Sse9I | AATT | 7 cut(s) | 234, 287, 369, 509, 516, 912, 1039 |
| SseBI | AGGCCT | 1 cut(s) | 302 |
| SsiI | CCGC | 1 cut(s) | 46 |
| SspMI | CTAG | 2 cut(s) | 633, 648 |
| StuI | AGGCCT | 1 cut(s) | 302 |
| StyD4I | CCNGG | 1 cut(s) | 225 |
| StyI | CCWWGG | 3 cut(s) | 266, 832, 903 |
| TaaI | ACNGT | 5 cut(s) | 161, 476, 541, 724, 751 |
| TaqI | TCGA | 7 cut(s) | 84, 114, 279, 573, 887, 897, 987 |
| TasI | AATT | 7 cut(s) | 234, 287, 369, 509, 516, 912, 1039 |
| TatI | WGTACW | 2 cut(s) | 258, 426 |
| TauI | GCSGC | 1 cut(s) | 48 |
| TfiI | GAWTC | 3 cut(s) | 39, 806, 828 |
| Tru1I | TTAA | 3 cut(s) | 141, 192, 441 |
| Tru9I | TTAA | 3 cut(s) | 141, 192, 441 |
| TscAI | CASTG | 4 cut(s) | 687, 727, 754, 847 |
| TseFI | GTSAC | 4 cut(s) | 52, 218, 875, 998 |
| TseI | GCWGC | 3 cut(s) | 332, 611, 673 |
| Tsp45I | GTSAC | 4 cut(s) | 52, 218, 875, 998 |
| TspDTI | ATGAA | 3 cut(s) | 195, 975, 1038 |
| TspGWI | ACGGA | 1 cut(s) | 332 |
| TspRI | CASTG | 4 cut(s) | 687, 727, 754, 847 |
| Vha464I | CTTAAG | 1 cut(s) | 191 |
| VpaK11BI | GGWCC | 1 cut(s) | 541 |
| XapI | RAATTY | 4 cut(s) | 234, 287, 509, 1039 |
| XceI | RCATGY | 1 cut(s) | 139 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 347 |
| XspI | CTAG | 2 cut(s) | 633, 648 |