Belongs to the glyceraldehyde-3-phosphate dehydrogenase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 450 |
| AciI | CCGC | 4 cut(s) | 252, 448, 559, 772 |
| AclWI | GGATC | 3 cut(s) | 350, 679, 692 |
| AcoI | YGGCCR | 1 cut(s) | 736 |
| AfaI | GTAC | 1 cut(s) | 244 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 32, 33, 162 |
| AflIII | ACRYGT | 1 cut(s) | 603 |
| AgsI | TTSAA | 1 cut(s) | 511 |
| AleI | CACNNNNGTG | 1 cut(s) | 429 |
| AluBI | AGCT | 2 cut(s) | 86, 172 |
| AluI | AGCT | 2 cut(s) | 86, 172 |
| Alw21I | GWGCWC | 2 cut(s) | 88, 174 |
| AlwI | GGATC | 3 cut(s) | 350, 679, 692 |
| Ama87I | CYCGRG | 1 cut(s) | 656 |
| AoxI | GGCC | 1 cut(s) | 736 |
| ApeKI | GCWGC | 1 cut(s) | 223 |
| AsuHPI | GGTGA | 1 cut(s) | 416 |
| AvaI | CYCGRG | 1 cut(s) | 656 |
| BaeGI | GKGCMC | 1 cut(s) | 522 |
| BalI | TGGCCA | 1 cut(s) | 738 |
| BamHI | GGATCC | 1 cut(s) | 684 |
| BanI | GGYRCC | 1 cut(s) | 450 |
| BanII | GRGCYC | 2 cut(s) | 88, 174 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 29, 61 |
| BauI | CACGAG | 1 cut(s) | 136 |
| Bbv12I | GWGCWC | 2 cut(s) | 88, 174 |
| BbvI | GCAGC | 1 cut(s) | 235 |
| BccI | CCATC | 1 cut(s) | 34 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 394, 428 |
| BfaI | CTAG | 1 cut(s) | 609 |
| BfmI | CTRYAG | 1 cut(s) | 346 |
| BglII | AGATCT | 1 cut(s) | 102 |
| BisI | GCNGC | 3 cut(s) | 224, 253, 449 |
| BlsI | GCNGC | 3 cut(s) | 225, 254, 450 |
| BmeT110I | CYCGRG | 1 cut(s) | 656 |
| BmiI | GGNNCC | 2 cut(s) | 452, 686 |
| BmrI | ACTGGG | 1 cut(s) | 21 |
| BmsI | GCATC | 2 cut(s) | 41, 62 |
| BmuI | ACTGGG | 1 cut(s) | 21 |
| BpmI | CTGGAG | 1 cut(s) | 771 |
| Bpu10I | CCTNAGC | 1 cut(s) | 10 |
| BpuEI | CTTGAG | 1 cut(s) | 219 |
| BsaJI | CCNNGG | 1 cut(s) | 657 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 32, 33, 162 |
| Bse118I | RCCGGY | 1 cut(s) | 226 |
| Bse1I | ACTGG | 2 cut(s) | 27, 788 |
| Bse3DI | GCAATG | 3 cut(s) | 22, 700, 709 |
| BseDI | CCNNGG | 1 cut(s) | 657 |
| BseGI | GGATG | 1 cut(s) | 56 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 32, 33, 162 |
| BseMI | GCAATG | 3 cut(s) | 22, 700, 709 |
| BseMII | CTCAG | 1 cut(s) | 24 |
| BseNI | ACTGG | 2 cut(s) | 27, 788 |
| BseSI | GKGCMC | 1 cut(s) | 522 |
| BseXI | GCAGC | 1 cut(s) | 235 |
| BshFI | GGCC | 1 cut(s) | 738 |
| BshNI | GGYRCC | 1 cut(s) | 450 |
| BsiHKAI | GWGCWC | 2 cut(s) | 88, 174 |
| BsiHKCI | CYCGRG | 1 cut(s) | 656 |
| BsiSI | CCGG | 1 cut(s) | 227 |
| BslFI | GGGAC | 2 cut(s) | 9, 575 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 32, 33, 162 |
| BsmFI | GGGAC | 2 cut(s) | 9, 575 |
| BsnI | GGCC | 1 cut(s) | 738 |
| BsoBI | CYCGRG | 1 cut(s) | 656 |
| Bsp1286I | GDGCHC | 3 cut(s) | 88, 174, 522 |
| Bsp143I | GATC | 4 cut(s) | 102, 160, 342, 684 |
| BspACI | CCGC | 4 cut(s) | 252, 448, 559, 772 |
| BspANI | GGCC | 1 cut(s) | 738 |
| BspCNI | CTCAG | 1 cut(s) | 23 |
| BspHI | TCATGA | 1 cut(s) | 331 |
| BspLI | GGNNCC | 2 cut(s) | 452, 686 |
| BspMAI | CTGCAG | 1 cut(s) | 350 |
| BspPI | GGATC | 3 cut(s) | 350, 679, 692 |
| BspT107I | GGYRCC | 1 cut(s) | 450 |
| BsrDI | GCAATG | 3 cut(s) | 22, 700, 709 |
| BsrFI | RCCGGY | 1 cut(s) | 226 |
| BsrI | ACTGG | 2 cut(s) | 27, 788 |
| BssAI | RCCGGY | 1 cut(s) | 226 |
| BssECI | CCNNGG | 1 cut(s) | 657 |
| BssMI | GATC | 4 cut(s) | 102, 160, 342, 684 |
| BssSI | CACGAG | 1 cut(s) | 136 |
| Bst2BI | CACGAG | 1 cut(s) | 136 |
| Bst4CI | ACNGT | 3 cut(s) | 329, 505, 649 |
| Bst6I | CTCTTC | 2 cut(s) | 614, 627 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 526, 699, 753 |
| BstC8I | GCNNGC | 2 cut(s) | 56, 413 |
| BstDEI | CTNAG | 1 cut(s) | 10 |
| BstF5I | GGATG | 1 cut(s) | 56 |
| BstKTI | GATC | 4 cut(s) | 105, 163, 345, 687 |
| BstMBI | GATC | 4 cut(s) | 102, 160, 342, 684 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 147, 526, 699, 744, 753 |
| BstNSI | RCATGY | 2 cut(s) | 145, 607 |
| BstSFI | CTRYAG | 1 cut(s) | 346 |
| BstSLI | GKGCMC | 1 cut(s) | 522 |
| BstV1I | GCAGC | 1 cut(s) | 235 |
| BstX2I | RGATCY | 3 cut(s) | 102, 342, 684 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 734 |
| BstYI | RGATCY | 3 cut(s) | 102, 342, 684 |
| BsuRI | GGCC | 1 cut(s) | 738 |
| BtsCI | GGATG | 1 cut(s) | 56 |
| BtsI | GCAGTG | 3 cut(s) | 376, 454, 751 |
| BtsIMutI | CAGTG | 5 cut(s) | 325, 376, 454, 600, 751 |
| Cac8I | GCNNGC | 2 cut(s) | 56, 413 |
| CciI | TCATGA | 1 cut(s) | 331 |
| Cfr10I | RCCGGY | 1 cut(s) | 226 |
| Csp6I | GTAC | 1 cut(s) | 243 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 370, 405 |
| CviAII | CATG | 6 cut(s) | 111, 142, 167, 332, 445, 604 |
| CviJI | RGCY | 8 cut(s) | 9, 86, 134, 172, 226, 233, 411, 738 |
| CviKI_1 | RGCY | 8 cut(s) | 9, 86, 134, 172, 226, 233, 411, 738 |
| CviQI | GTAC | 1 cut(s) | 243 |
| DdeI | CTNAG | 1 cut(s) | 10 |
| DpnI | GATC | 4 cut(s) | 104, 162, 344, 686 |
| DpnII | GATC | 4 cut(s) | 102, 160, 342, 684 |
| EaeI | YGGCCR | 1 cut(s) | 736 |
| Eam1104I | CTCTTC | 2 cut(s) | 614, 627 |
| EarI | CTCTTC | 2 cut(s) | 614, 627 |
| Ecl136II | GAGCTC | 2 cut(s) | 86, 172 |
| Eco24I | GRGCYC | 2 cut(s) | 88, 174 |
| Eco53kI | GAGCTC | 2 cut(s) | 86, 172 |
| Eco88I | CYCGRG | 1 cut(s) | 656 |
| EcoICRI | GAGCTC | 2 cut(s) | 86, 172 |
| EcoT22I | ATGCAT | 1 cut(s) | 531 |
| EcoT38I | GRGCYC | 2 cut(s) | 88, 174 |
| FaeI | CATG | 6 cut(s) | 114, 145, 170, 335, 448, 607 |
| FaqI | GGGAC | 2 cut(s) | 9, 575 |
| FatI | CATG | 6 cut(s) | 110, 141, 166, 331, 444, 603 |
| FauI | CCCGC | 2 cut(s) | 552, 779 |
| Fnu4HI | GCNGC | 3 cut(s) | 224, 253, 449 |
| FokI | GGATG | 1 cut(s) | 63 |
| FriOI | GRGCYC | 2 cut(s) | 88, 174 |
| Fsp4HI | GCNGC | 3 cut(s) | 224, 253, 449 |
| FspBI | CTAG | 1 cut(s) | 609 |
| GluI | GCNGC | 3 cut(s) | 224, 253, 449 |
| GsuI | CTGGAG | 1 cut(s) | 771 |
| HaeIII | GGCC | 1 cut(s) | 738 |
| HapII | CCGG | 1 cut(s) | 227 |
| Hin1II | CATG | 6 cut(s) | 114, 145, 170, 335, 448, 607 |
| HinfI | GANTC | 3 cut(s) | 79, 351, 485 |
| HpaII | CCGG | 1 cut(s) | 227 |
| HphI | GGTGA | 1 cut(s) | 416 |
| Hpy166II | GTNNAC | 2 cut(s) | 365, 721 |
| Hpy188I | TCNGA | 1 cut(s) | 669 |
| Hpy188III | TCNNGA | 3 cut(s) | 332, 362, 609 |
| Hpy8I | GTNNAC | 2 cut(s) | 365, 721 |
| HpyAV | CCTTC | 2 cut(s) | 60, 670 |
| HpyCH4III | ACNGT | 3 cut(s) | 329, 505, 649 |
| HpyCH4IV | ACGT | 2 cut(s) | 22, 614 |
| HpyCH4V | TGCA | 9 cut(s) | 54, 288, 348, 381, 529, 693, 702, 747, 756 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 147, 526, 699, 744, 753 |
| HpyF3I | CTNAG | 1 cut(s) | 10 |
| HpySE526I | ACGT | 2 cut(s) | 22, 614 |
| Hsp92II | CATG | 6 cut(s) | 114, 145, 170, 335, 448, 607 |
| Kzo9I | GATC | 4 cut(s) | 102, 160, 342, 684 |
| LmnI | GCTCC | 2 cut(s) | 91, 590 |
| LpnPI | CCDG | 6 cut(s) | 40, 89, 240, 440, 701, 752 |
| Lsp1109I | GCAGC | 1 cut(s) | 235 |
| LweI | GCATC | 2 cut(s) | 41, 62 |
| MaeI | CTAG | 1 cut(s) | 609 |
| MaeII | ACGT | 2 cut(s) | 22, 614 |
| MaeIII | GTNAC | 5 cut(s) | 44, 95, 122, 212, 539 |
| MalI | GATC | 4 cut(s) | 104, 162, 344, 686 |
| MboI | GATC | 4 cut(s) | 102, 160, 342, 684 |
| MboII | GAAGA | 4 cut(s) | 49, 631, 644, 785 |
| MfeI | CAATTG | 1 cut(s) | 777 |
| MflI | RGATCY | 3 cut(s) | 102, 342, 684 |
| MhlI | GDGCHC | 3 cut(s) | 88, 174, 522 |
| MlsI | TGGCCA | 1 cut(s) | 738 |
| MluCI | AATT | 2 cut(s) | 320, 777 |
| MluNI | TGGCCA | 1 cut(s) | 738 |
| MlyI | GAGTC | 1 cut(s) | 479 |
| MnlI | CCTC | 2 cut(s) | 652, 795 |
| Mox20I | TGGCCA | 1 cut(s) | 738 |
| Mph1103I | ATGCAT | 1 cut(s) | 531 |
| MscI | TGGCCA | 1 cut(s) | 738 |
| MseI | TTAA | 1 cut(s) | 189 |
| MslI | CAYNNNNRTG | 4 cut(s) | 140, 330, 336, 429 |
| Msp20I | TGGCCA | 1 cut(s) | 738 |
| MspI | CCGG | 1 cut(s) | 227 |
| MunI | CAATTG | 1 cut(s) | 777 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 147, 526, 699, 744, 753 |
| NdeII | GATC | 4 cut(s) | 102, 160, 342, 684 |
| NlaIII | CATG | 6 cut(s) | 114, 145, 170, 335, 448, 607 |
| NlaIV | GGNNCC | 2 cut(s) | 452, 686 |
| NmuCI | GTSAC | 2 cut(s) | 122, 212 |
| NsiI | ATGCAT | 1 cut(s) | 531 |
| NspI | RCATGY | 2 cut(s) | 145, 607 |
| OliI | CACNNNNGTG | 1 cut(s) | 429 |
| PagI | TCATGA | 1 cut(s) | 331 |
| PciI | ACATGT | 1 cut(s) | 603 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 180 |
| PfeI | GAWTC | 2 cut(s) | 79, 351 |
| PkrI | GCNGC | 3 cut(s) | 225, 254, 450 |
| PleI | GAGTC | 1 cut(s) | 479 |
| PpsI | GAGTC | 1 cut(s) | 479 |
| PscI | ACATGT | 1 cut(s) | 603 |
| Psp124BI | GAGCTC | 2 cut(s) | 88, 174 |
| PspN4I | GGNNCC | 2 cut(s) | 452, 686 |
| PstI | CTGCAG | 1 cut(s) | 350 |
| PsuI | RGATCY | 3 cut(s) | 102, 342, 684 |
| RsaI | GTAC | 1 cut(s) | 244 |
| RsaNI | GTAC | 1 cut(s) | 243 |
| RseI | CAYNNNNRTG | 4 cut(s) | 140, 330, 336, 429 |
| SacI | GAGCTC | 2 cut(s) | 88, 174 |
| SaqAI | TTAA | 1 cut(s) | 189 |
| SatI | GCNGC | 3 cut(s) | 224, 253, 449 |
| Sau3AI | GATC | 4 cut(s) | 102, 160, 342, 684 |
| SchI | GAGTC | 1 cut(s) | 479 |
| SduI | GDGCHC | 3 cut(s) | 88, 174, 522 |
| SetI | ASST | 6 cut(s) | 25, 88, 174, 429, 617, 681 |
| SfaNI | GCATC | 2 cut(s) | 41, 62 |
| SfcI | CTRYAG | 1 cut(s) | 346 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 140, 330, 336, 429 |
| SmlI | CTYRAG | 1 cut(s) | 234 |
| SmoI | CTYRAG | 1 cut(s) | 234 |
| Sse9I | AATT | 2 cut(s) | 320, 777 |
| SsiI | CCGC | 4 cut(s) | 252, 448, 559, 772 |
| SspI | AATATT | 2 cut(s) | 272, 569 |
| SspMI | CTAG | 1 cut(s) | 609 |
| SstI | GAGCTC | 2 cut(s) | 88, 174 |
| TaaI | ACNGT | 3 cut(s) | 329, 505, 649 |
| TaiI | ACGT | 2 cut(s) | 25, 617 |
| TaqI | TCGA | 2 cut(s) | 82, 404 |
| TasI | AATT | 2 cut(s) | 320, 777 |
| TauI | GCSGC | 2 cut(s) | 255, 451 |
| TfiI | GAWTC | 2 cut(s) | 79, 351 |
| Tru1I | TTAA | 1 cut(s) | 189 |
| Tru9I | TTAA | 1 cut(s) | 189 |
| TscAI | CASTG | 5 cut(s) | 332, 383, 461, 600, 758 |
| TseFI | GTSAC | 2 cut(s) | 122, 212 |
| TseI | GCWGC | 1 cut(s) | 223 |
| Tsp45I | GTSAC | 2 cut(s) | 122, 212 |
| TspDTI | ATGAA | 3 cut(s) | 33, 144, 645 |
| TspGWI | ACGGA | 2 cut(s) | 63, 78 |
| TspRI | CASTG | 5 cut(s) | 332, 383, 461, 600, 758 |
| XbaI | TCTAGA | 1 cut(s) | 608 |
| XceI | RCATGY | 2 cut(s) | 145, 607 |
| XspI | CTAG | 1 cut(s) | 609 |
| Zsp2I | ATGCAT | 1 cut(s) | 531 |