Rh1AG445000

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000502
Physical Location & Seq
Reverse (-)
66536803 .. 66542953
6151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG445000.1

Sequence Viewer

Length: 3510 bp
ATGAATCGCTCCCATCTAGCTCTTATTCCCAAGGTTAAAGCTCCGGTAAATGCTACTCATTTTCGTCCAATAGCCCTCTGTAACTTTTCTTACAAGATTCTGACCAAGATCATTGTGAATCGTTTGAAACCTTTCATGGCAAGCCTTATCACTGAGAATCAGTCAGCCTTTGTCTCAGACAGACAAATTCAAGACAATATTGTTGTTGCTCATGAACTTTTTCATCAGTTAAAGCTCCGCCGATCTGGGAACAATGCAGCTTTTGGTTTAAAGCTAGATATGAATAAGGCTTATGATCGTATTGAGTGGGACTTCTTGCAGGCAGTCTTAAAAAAGATGGGTTTTGCAGATCAATGGGTGACTTTGGTAATGAATTGTGTCCGAACTTCCTCCCTCTCTGTGCTTTTTAATGGGAAACCAGGGCCTTTTTTCAAACCAACCCGTGGTCTCAGACAGGGAGATCCCTTGTCACCTTTTCTCTTTCTTTTTGTTAATGACGTTCTTTCAAAAATGGTCATCAAATGTTGCCAATGCTCCCTACTGAAACCTGTAACTATTGGTCCACAAGAATTAGGAATCAGTCATCTTTTCTTTGCAGATGACTCCCTGTTCTTCCTTCAAGCCACTCTACAGAACTGTGAAATGTTGTCTGATCTCCTCCACACTTATTGTGTAGCCTCTGGTCAATTGATTAATGTGGACAAATCTTCTATTTTTTTCAGTCCAAATACTCTACCTGAAATTGTACATTTGTTGAGTGCTATTATGCAAATCCCGGTTGTTTTTGATCCAGGAAAATACTTGGGGCTTCCAACCTTCTGGCATCGATCAAAAAAGGCTGCTCTTGGTTTTATTAAGGACTCAGTATCCAAGAAAGTTCAGGGATGGAAACAGGCAACTCTTAGCCAAGCTGGGAAAGAAACTCTCATCAAGGCTATAGCCACGGCTATCCCAGCTTATCCTATGGCATGTTTTAAATTTCCGGTCTCTCTTTGCACTCAATTGAATGGTATTTTGGCAAATTTTTGGTGGGGAAATCAAGATTCTAATGGACTGCATTGGAAGTCTTGGAATTTCCTAAGTTTGCCTAAACATGACGGTGGAATGGGATTCAGAAATTTGGAAGAATTCAATGATTCATTGTTGGCTAAACAAGCTTGGAGACTTACTCATAATCCCACTTCTTTATGGGCTCGTGTCCTGCAGCAACTGTATTTTCCATTGACTTCTTTTCGTTTCGCAAGAAAAGGAGCTTCTGCTTCTTGGATTTGGTCAAGTCTGTTGATTGGTAGAGAAGTTATTCATTCTGAATCTCTTTGGAACATTGGTAATGGTTCTTCTGTTCATATCGGAAGTGATACATGGATCCCTTCCAACCCTCCTTCTTGGATCCATCTTCCGCTGGACCGTAGGAACTTTCAGGTTGGTTCTCTCATCAATTGGGACACAAAAACGTGGAATCTGGACCAAGTCAGAGACCTCATTACACCTGCTCAATCCCTCTTGATTTCTGCTATTCCTGTTACTGATCAAAATATTCCTGATACGTTGATATGGCCTCACACCAGAAACGGTGGTTTCACTGTTAAGTCAGGCTATCATCTCCTTCACTCTCGTTCTCAAGTTGGAGTAATGCAACATCCTCATAATTCATCTGCTATTCCTACCAGTCTTTGGTCTTGGATTTGGAAGCTTCACACAATTCCTCGAGTCAAAAATTTCTTATGGCGTGCTTGTCATGGTATCCTTCCAACAAAGAGCAACTTATTTCGAAGGCATATTGCATCTTCAGCTGAGTGTCAATTATGCCTGCACCCAAATGAATCAACTGAACATGCTCTTCTCACTTGTCCTTGGATTGTAAGCGCTTGGTTTGCTCATCCCCTATCTTACAAAGTTCCTCTACAAGCTATCACTTCTTTTGAAAGTTGGCTCTTCAACATAAGCCAAATGGATACCCCTGCTGATTTTGTCACCCATGTTGTGTTCTTTCTGTGGAGGATTTGGAAGCATAGATGTGATTGTTTTTTTAATAAGCTTTTCCCAGATCCAATCAAGATTGCTTTGTCTGCTTTCAAAGCAGCGAGTGATTTCCTTGCAGCTCACTCATTAGAGAGTTTAAATTCAGATTTGGGGTGTCACACCCTTCCCAATTCTATTCCTGTTAAGTGGTCTCCCCCCCCTGTCAATGCCATCCTCATTAATACTGATGCTTCTTGGCAGAAGGACACCCTTATGTGTGGAGTGGCAGCTTTGGCTAGAAATTGGAAAGGTTCTTTGATTGCCGGTAAAACTGCAAAATTGCTTGCTCCTTCCCCTTTGGCAGCAGAGGCATTAGCAGTTCGGGAAGCCATTCATCTGGCAAACTCCTTCCCTCATATGGATATCATTATCTCTTCAGATTCTCAAACTCTAATCAACTCCATCTCCCGTAATCTCCCTGTATCTGATTGGAAAGCTGCAACTATTATTTCTCAAGTCCGGTACCTCTCCTCAACCCGGCAGTTTAACTGGTCTTGGATCAGTAGAAAGGCTAATAAAGCAGCCCATCTTGTTGCTTCATTAGTTATCAGGAAAATCAAACTCGGAATCAATGGGTTTGGAAGAATTGGAAGATTGGTCACAAGAGTGGCTTTGCAAAGAGACGATATTGAGCTTGTTTGCATTAATGACCCCTACATCACGGCCGACTATATTGTCTACATGTTCAAGCACGACACTGTTCACGGTGCATGGAAGAATTGCGATGTGAAACTTAAGAATGCAAATCACGTTTACTTTGGTAACAAGGTAGTCACCATTTTTGCACTCAGCCGAGAAGACGAAATCCCTTGGTCTGGCCCCGGAGCCGAATACATCGTGGATGCCTCTGGTACTGCCACCGAAATCGAATTTGCTCAGGGCCATTTGAAGGGCGGAGCAAAGAAGGTTATTGTCACTTGCCCGTGTGATGGTGCTCCTACGTTTGTTGTGGGTGTCAATGAGAAGGAATACAAGCCGGAGCTTAATGTTATTTCCGGTGCTAGTTGCACCACCAACTGCCTTGCCCCCCTTGCAAAGGTAATTCATGATAAATTTGGTATCATCGAGGGTCTGATGAGCACTGTTCACTCAGTTACTGCTAGCCAAAGAGGTGTTGATGGAACATGTCCTAATGATTGGAGACCTGGAAAAGCTGCACCAACTATCAAAGATTGGAGAATTGGAAGAGCTGCTTATATAAACATCATTCCCACTACTACCGGAGCTGCTAAGGTTGTTGGTAAAGTTCTACCTGACCTGGATGGTAAATTGACCGGAATGTCATTCCGTGTTCCTAGTCTTGATGTTTCGGTGGTAGACCTAACAGTGAGACTAGAAAAGCCGGCTACGTATGAGGAAATAAAAGCTGCCATCAAGGAAGAGTCTGAGGGAAACCTGAAGGGAATCTTGGGTTACACTGAAGATGATGTGGTCTCTACTGACTTTGAGCGTGACAGCAGGTACACAAATGCATATATATGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1169

Amino Acids

130.4

Weight (kDa)

9.15

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 11 - 270 3.9e-29 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 527 - 624 5.9e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 735 - 854 7.6e-15 Reverse transcriptase-like
Gp_dh_N PF00044 860 - 961 8.3e-25 Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
Gp_dh_C PF02800 1015 - 1060 4.7e-12 Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Gp_dh_C PF02800 1062 - 1163 2.5e-38 Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13440 AT1G13440 AT3G04120
fragaria_vesca FvH4_4g24420 FvH4_5g07640 FvH4_6g52741 FvH4_6g52755
malus_domestica MD06G1148800.v1.1 MD09G1016900.v1.1 MD13G1111500.v1.1 MD16G1111100.v1.1 MD17G1017300.v1.1
prunus_persica Prupe.1G234000_v2.0.a1 Prupe.1G234000_v2.0.a1 Prupe.1G234000_v2.0.a1 Prupe.3G300600_v2.0.a1 Prupe.5G155800_v2.0.a1 Prupe.5G155800_v2.0.a1
pyrus_communis pycom06g13820 pycom111g01370 pycom13g09680 pycom16g09410
rosa_chinensis RchiOBHm_Chr1g0380411 RchiOBHm_Chr2g0174371 RchiOBHm_Chr2g0174391 RchiOBHm_Chr2g0174401 RchiOBHm_Chr4g0431271 RchiOBHm_Chr7g0190831 RchiOBHm_Chr7g0209601
rosa_laevigata RLG00000003113 RLG00000004459 RLG00000004490 RLG00000022261 RLG00000022262 RLG00000026305
rosa_multiflora Rmu_sc0000033.1_g000020 Rmu_sc0000243.1_g000023 Rmu_sc0002637.1_g000006 Rmu_sc0002637.1_g000007 Rmu_sc0004033.1_g000020 Rmu_sc0004787.1_g000001 Rmu_sc0005371.1_g000004 Rmu_sc0007885.1_g000015 Rmu_sc0019128.1_g000001
rosa_roxburghii Rroxscaffold_2G00078250 Rroxscaffold_2G00078260 Rroxscaffold_3G00249220 Rroxscaffold_3G00264260 Rroxscaffold_4G00279410 Rroxscaffold_5G00372820
rosa_rugosa Rorug01G0420800 Rorug04G0249800 Rorug04G0249900 Rorug06G0507500 Rorug07G0114500.1
rosa_samantha Rh1AG445000 Rh1BG400200 Rh1CG414800 Rh1DG430100 Rh2AG657500 Rh2AG657600 Rh2BG668500 Rh2BG668600 Rh2BG668700 Rh2CG631600 Rh2DG682100 Rh4AG305700 Rh4BG313100 Rh4CG329300 Rh4DG309500 Rh5AG149300 Rh5DG147700 Rh7AG114100 Rh7AG248500 Rh7BG116700 Rh7BG242600 Rh7CG119400 Rh7CG264500 Rh7DG117900 Rh7DG254400
rosa_wichuraiana Rw1G038730 Rw2G053850 Rw2G053860 Rw4G026580 Rw5G013240 Rw7G021060 Rw7G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1498
AasI GACNNNNNNGTC 2 cut(s) 2696, 3301
Acc36I ACCTGC 2 cut(s) 1498, 3471
Acc65I GGTACC 1 cut(s) 2484
AccB1I GGYRCC 1 cut(s) 2484
AccB7I CCANNNNNTGG 2 cut(s) 443, 1674
AccI GTMKAC 2 cut(s) 2700, 3339
AciI CCGC 3 cut(s) 238, 1400, 2916
AclWI GGATC 8 cut(s) 455, 782, 1360, 1373, 1384, 1397, 2042, 2528
AcoI YGGCCR 1 cut(s) 2685
AcuI CTGAAG 4 cut(s) 1773, 2382, 3440, 3462
AfaI GTAC 4 cut(s) 747, 2486, 2875, 3485
AfeI AGCGCT 1 cut(s) 1867
AfiI CCNNNNNNNGG 8 cut(s) 443, 1674, 2380, 2499, 2837, 2911, 2951, 3058
AflII CTTAAG 1 cut(s) 2756
AflIII ACRYGT 2 cut(s) 2703, 3146
AjnI CCWGG 4 cut(s) 418, 790, 3166, 3279
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 3413, 3445
AleI CACNNNNGTG 1 cut(s) 2627
Alw21I GWGCWC 2 cut(s) 2959, 3104
AlwI GGATC 8 cut(s) 455, 782, 1360, 1373, 1384, 1397, 2042, 2528
AlwNI CAGNNNCTG 2 cut(s) 1210, 3119
Ama87I CYCGRG 1 cut(s) 1707
Aor51HI AGCGCT 1 cut(s) 1867
AoxI GGCC 5 cut(s) 422, 1556, 2685, 2839, 2902
AseI ATTAAT 3 cut(s) 693, 2202, 2667
Asp700I GAANNNNTTC 4 cut(s) 131, 219, 1299, 2352
Asp718I GGTACC 1 cut(s) 2484
AspLEI GCGC 1 cut(s) 1868
AspS9I GGNCC 6 cut(s) 422, 560, 1405, 1465, 2840, 2902
AsuC2I CCSGG 3 cut(s) 776, 2500, 2844
AsuHPI GGTGA 4 cut(s) 370, 462, 1966, 2788
AsuII TTCGAA 1 cut(s) 1771
AsuNHI GCTAGC 1 cut(s) 3122
AvaI CYCGRG 1 cut(s) 1707
AvaII GGWCC 3 cut(s) 560, 1405, 1465
BamHI GGATCC 2 cut(s) 1365, 1389
BanI GGYRCC 1 cut(s) 2484
BanII GRGCYC 1 cut(s) 1195
BarI GAAGNNNNNNTAC 2 cut(s) 1321, 1353
BauI CACGAG 1 cut(s) 1194
BbsI GAAGAC 1 cut(s) 2826
Bbv12I GWGCWC 2 cut(s) 2959, 3104
BceAI ACGGC 2 cut(s) 960, 2700
BciT130I CCWGG 4 cut(s) 420, 792, 3168, 3281
BciVI GTATCC 3 cut(s) 877, 1754, 1948
BclI TGATCA 1 cut(s) 1528
BcnI CCSGG 3 cut(s) 776, 2500, 2844
BfaI CTAG 7 cut(s) 17, 275, 2259, 3024, 3123, 3318, 3356
BfmI CTRYAG 3 cut(s) 629, 936, 1202
BfoI RGCGCY 1 cut(s) 1869
BfrI CTTAAG 1 cut(s) 2756
BfuAI ACCTGC 2 cut(s) 1498, 3471
BfuI GTATCC 3 cut(s) 877, 1754, 1948
Bme1390I CCNGG 7 cut(s) 420, 776, 792, 2500, 2844, 3168, 3281
Bme18I GGWCC 3 cut(s) 560, 1405, 1465
BmeT110I CYCGRG 1 cut(s) 1707
BmgT120I GGNCC 6 cut(s) 422, 560, 1405, 1465, 2840, 2902
BmiI GGNNCC 5 cut(s) 1367, 1391, 2486, 2842, 2848
BmrFI CCNGG 7 cut(s) 420, 776, 792, 2500, 2844, 3168, 3281
BmsI GCATC 4 cut(s) 832, 1793, 2200, 2854
BmtI GCTAGC 1 cut(s) 3126
BpiI GAAGAC 1 cut(s) 2826
BplI GAGNNNNNCTC 2 cut(s) 1153, 1185
Bpu10I CCTNAGC 2 cut(s) 2898, 3252
Bpu14I TTCGAA 1 cut(s) 1771
BpuEI CTTGAG 2 cut(s) 1605, 2460
BpuMI CCSGG 3 cut(s) 776, 2500, 2844
Bsa29I ATCGAT 1 cut(s) 826
BsaAI YACGTR 1 cut(s) 3372
BsaI GGTCTC 6 cut(s) 452, 991, 1470, 2178, 3157, 3460
BsaJI CCNNGG 7 cut(s) 30, 419, 442, 942, 1853, 2831, 2842
BsaWI WCCGGW 6 cut(s) 43, 982, 2481, 3017, 3242, 3296
BsaXI ACNNNNNCTCC 2 cut(s) 2411, 2441
Bsc4I CCNNNNNNNGG 8 cut(s) 443, 1674, 2380, 2499, 2837, 2911, 2951, 3058
Bse118I RCCGGY 2 cut(s) 2285, 3364
Bse1I ACTGG 2 cut(s) 1668, 2516
BseBI CCWGG 4 cut(s) 420, 792, 3168, 3281
BseCI ATCGAT 1 cut(s) 826
BseDI CCNNGG 7 cut(s) 30, 419, 442, 942, 1853, 2831, 2842
BseGI GGATG 6 cut(s) 890, 1639, 1879, 2193, 2869, 3289
BseLI CCNNNNNNNGG 8 cut(s) 443, 1674, 2380, 2499, 2837, 2911, 2951, 3058
BseMII CTCAG 9 cut(s) 144, 189, 463, 876, 1785, 2824, 2912, 3126, 3399
BseNI ACTGG 2 cut(s) 1668, 2516
BseRI GAGGAG 2 cut(s) 647, 2482
BseX3I CGGCCG 1 cut(s) 2685
BseYI CCCAGC 2 cut(s) 911, 952
BsgI GTGCAG 2 cut(s) 1796, 3162
Bsh1285I CGRYCG 1 cut(s) 2688
BshFI GGCC 5 cut(s) 424, 1558, 2687, 2841, 2904
BshNI GGYRCC 1 cut(s) 2484
BshVI ATCGAT 1 cut(s) 826
BsiEI CGRYCG 1 cut(s) 2688
BsiHKAI GWGCWC 2 cut(s) 2959, 3104
BsiHKCI CYCGRG 1 cut(s) 1707
BslFI GGGAC 2 cut(s) 323, 1457
BslI CCNNNNNNNGG 8 cut(s) 443, 1674, 2380, 2499, 2837, 2911, 2951, 3058
BsmBI CGTCTC 1 cut(s) 2637
BsmFI GGGAC 2 cut(s) 323, 1457
BsmI GAATGC 1 cut(s) 2767
BsnI GGCC 5 cut(s) 424, 1558, 2687, 2841, 2904
Bso31I GGTCTC 6 cut(s) 452, 991, 1470, 2178, 3157, 3460
BsoBI CYCGRG 1 cut(s) 1707
Bsp119I TTCGAA 1 cut(s) 1771
Bsp1286I GDGCHC 3 cut(s) 1195, 2959, 3104
Bsp1407I TGTACA 1 cut(s) 745
BspACI CCGC 3 cut(s) 238, 1400, 2916
BspANI GGCC 5 cut(s) 424, 1558, 2687, 2841, 2904
BspCNI CTCAG 9 cut(s) 145, 188, 462, 875, 1786, 2823, 2911, 3125, 3400
BspDI ATCGAT 1 cut(s) 826
BspHI TCATGA 2 cut(s) 211, 3067
BspLI GGNNCC 5 cut(s) 1367, 1391, 2486, 2842, 2848
BspMAI CTGCAG 1 cut(s) 1206
BspMI ACCTGC 2 cut(s) 1498, 3471
BspOI GCTAGC 1 cut(s) 3126
BspPI GGATC 8 cut(s) 455, 782, 1360, 1373, 1384, 1397, 2042, 2528
BspQI GCTCTTC 3 cut(s) 1845, 1940, 3202
BspT104I TTCGAA 1 cut(s) 1771
BspT107I GGYRCC 1 cut(s) 2484
BspTI CTTAAG 1 cut(s) 2756
BspTNI GGTCTC 6 cut(s) 452, 991, 1470, 2178, 3157, 3460
BsrFI RCCGGY 2 cut(s) 2285, 3364
BsrGI TGTACA 1 cut(s) 745
BsrI ACTGG 2 cut(s) 1668, 2516
BssAI RCCGGY 2 cut(s) 2285, 3364
BssECI CCNNGG 7 cut(s) 30, 419, 442, 942, 1853, 2831, 2842
BssSI CACGAG 1 cut(s) 1194
BssT1I CCWWGG 3 cut(s) 30, 1853, 2831
Bst2BI CACGAG 1 cut(s) 1194
Bst2UI CCWGG 4 cut(s) 420, 792, 3168, 3281
Bst6I CTCTTC 5 cut(s) 1845, 1940, 2401, 3202, 3396
BstAFI CTTAAG 1 cut(s) 2756
BstAUI TGTACA 1 cut(s) 745
BstBAI YACGTR 1 cut(s) 3372
BstBI TTCGAA 1 cut(s) 1771
BstC8I GCNNGC 7 cut(s) 142, 321, 1731, 1811, 2307, 3124, 3366
BstDSI CCRYGG 2 cut(s) 442, 942
BstENI CCTNNNNNAGG 1 cut(s) 3056
BstF5I GGATG 6 cut(s) 890, 1639, 1879, 2193, 2869, 3289
BstH2I RGCGCY 1 cut(s) 1869
BstHHI GCGC 1 cut(s) 1868
BstMCI CGRYCG 1 cut(s) 2688
BstNI CCWGG 4 cut(s) 420, 792, 3168, 3281
BstNSI RCATGY 4 cut(s) 972, 1838, 2707, 3150
BstSCI CCNGG 7 cut(s) 418, 774, 790, 2498, 2842, 3166, 3279
BstSFI CTRYAG 3 cut(s) 629, 936, 1202
BstSNI TACGTA 1 cut(s) 3372
BstV2I GAAGAC 1 cut(s) 2826
BstX2I RGATCY 4 cut(s) 460, 1365, 1389, 2047
BstXI CCANNNNNNTGG 2 cut(s) 819, 2359
BstYI RGATCY 4 cut(s) 460, 1365, 1389, 2047
BstZI CGGCCG 1 cut(s) 2685
Bsu15I ATCGAT 1 cut(s) 826
BsuI GTATCC 3 cut(s) 877, 1754, 1948
BsuRI GGCC 5 cut(s) 424, 1558, 2687, 2841, 2904
BsuTUI ATCGAT 1 cut(s) 826
BtgI CCRYGG 2 cut(s) 442, 942
BtgZI GCGATG 1 cut(s) 2760
BtsCI GGATG 6 cut(s) 890, 1639, 1879, 2193, 2869, 3289
BtsIMutI CAGTG 6 cut(s) 150, 1581, 2718, 3102, 3354, 3438
BveI ACCTGC 2 cut(s) 1498, 3471
Cac8I GCNNGC 7 cut(s) 142, 321, 1731, 1811, 2307, 3124, 3366
CaiI CAGNNNCTG 2 cut(s) 1210, 3119
CciI TCATGA 2 cut(s) 211, 3067
CfoI GCGC 1 cut(s) 1868
Cfr10I RCCGGY 2 cut(s) 2285, 3364
Cfr13I GGNCC 6 cut(s) 422, 560, 1405, 1465, 2840, 2902
ClaI ATCGAT 1 cut(s) 826
Csp6I GTAC 4 cut(s) 746, 2485, 2874, 3484
CspCI CAANNNNNGTGG 2 cut(s) 650, 685
CviQI GTAC 4 cut(s) 746, 2485, 2874, 3484
DraI TTTAAA 3 cut(s) 270, 976, 2121
DrdI GACNNNNNNGTC 2 cut(s) 2696, 3301
DseDI GACNNNNNNGTC 2 cut(s) 2696, 3301
EaeI YGGCCR 1 cut(s) 2685
EagI CGGCCG 1 cut(s) 2685
Eam1104I CTCTTC 5 cut(s) 1845, 1940, 2401, 3202, 3396
EarI CTCTTC 5 cut(s) 1845, 1940, 2401, 3202, 3396
EciI GGCGGA 2 cut(s) 227, 2931
EclXI CGGCCG 1 cut(s) 2685
Eco105I TACGTA 1 cut(s) 3372
Eco130I CCWWGG 3 cut(s) 30, 1853, 2831
Eco24I GRGCYC 1 cut(s) 1195
Eco31I GGTCTC 6 cut(s) 452, 991, 1470, 2178, 3157, 3460
Eco32I GATATC 1 cut(s) 2386
Eco47I GGWCC 3 cut(s) 560, 1405, 1465
Eco47III AGCGCT 1 cut(s) 1867
Eco52I CGGCCG 1 cut(s) 2685
Eco57I CTGAAG 4 cut(s) 1773, 2382, 3440, 3462
Eco88I CYCGRG 1 cut(s) 1707
EcoNI CCTNNNNNAGG 1 cut(s) 3056
EcoO109I RGGNCCY 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 1127
EcoRII CCWGG 4 cut(s) 418, 790, 3166, 3279
EcoRV GATATC 1 cut(s) 2386
EcoT14I CCWWGG 3 cut(s) 30, 1853, 2831
EcoT22I ATGCAT 1 cut(s) 3496
EcoT38I GRGCYC 1 cut(s) 1195
ErhI CCWWGG 3 cut(s) 30, 1853, 2831
Esp3I CGTCTC 1 cut(s) 2637
FalI AAGNNNNNCTT 8 cut(s) 1748, 1780, 2617, 2649, 3199, 3231, 3413, 3445
FaqI GGGAC 2 cut(s) 323, 1457
FauNDI CATATG 1 cut(s) 2379
FbaI TGATCA 1 cut(s) 1528
FblI GTMKAC 2 cut(s) 2700, 3339
FokI GGATG 6 cut(s) 897, 1626, 1866, 2180, 2876, 3296
FriOI GRGCYC 1 cut(s) 1195
FspBI CTAG 7 cut(s) 17, 275, 2259, 3024, 3123, 3318, 3356
GlaI GCGC 1 cut(s) 1867
GsaI CCCAGC 2 cut(s) 915, 956
HaeII RGCGCY 1 cut(s) 1869
HaeIII GGCC 5 cut(s) 424, 1558, 2687, 2841, 2904
HhaI GCGC 1 cut(s) 1868
Hin6I GCGC 1 cut(s) 1866
HinP1I GCGC 1 cut(s) 1866
HindIII AAGCTT 3 cut(s) 1155, 1691, 2036
HphI GGTGA 4 cut(s) 370, 462, 1966, 2788
Hpy166II GTNNAC 8 cut(s) 563, 700, 2701, 2725, 2776, 3109, 3340, 3486
Hpy8I GTNNAC 8 cut(s) 563, 700, 2701, 2725, 2776, 3109, 3340, 3486
HpyCH4IV ACGT 6 cut(s) 498, 1454, 1547, 2772, 2963, 3371
HpySE526I ACGT 6 cut(s) 498, 1454, 1547, 2772, 2963, 3371
HspAI GCGC 1 cut(s) 1866
KpnI GGTACC 1 cut(s) 2488
KroI GCCGGC 1 cut(s) 3364
KroNI GCCGGC 1 cut(s) 3366
Ksp22I TGATCA 1 cut(s) 1528
LguI GCTCTTC 3 cut(s) 1845, 1940, 3202
LweI GCATC 4 cut(s) 832, 1793, 2200, 2854
MaeI CTAG 7 cut(s) 17, 275, 2259, 3024, 3123, 3318, 3356
MaeII ACGT 6 cut(s) 498, 1454, 1547, 2772, 2963, 3371
MfeI CAATTG 3 cut(s) 686, 1001, 1438
MflI RGATCY 4 cut(s) 460, 1365, 1389, 2047
MhlI GDGCHC 3 cut(s) 1195, 2959, 3104
MlyI GAGTC 4 cut(s) 596, 854, 1719, 3413
MmeI TCCRAC 4 cut(s) 836, 1398, 1606, 1775
Mph1103I ATGCAT 1 cut(s) 3496
MroNI GCCGGC 1 cut(s) 3364
MroXI GAANNNNTTC 4 cut(s) 131, 219, 1299, 2352
MslI CAYNNNNRTG 6 cut(s) 1098, 1818, 2016, 2234, 2627, 3499
MspA1I CMGCKG 2 cut(s) 1402, 1793
MspCI CTTAAG 1 cut(s) 2756
MspR9I CCNGG 7 cut(s) 420, 776, 792, 2500, 2844, 3168, 3281
MunI CAATTG 3 cut(s) 686, 1001, 1438
Mva1269I GAATGC 1 cut(s) 2767
MvaI CCWGG 4 cut(s) 420, 792, 3168, 3281
NaeI GCCGGC 1 cut(s) 3366
NciI CCSGG 3 cut(s) 776, 2500, 2844
NdeI CATATG 1 cut(s) 2379
NgoMIV GCCGGC 1 cut(s) 3364
NheI GCTAGC 1 cut(s) 3122
NlaIV GGNNCC 5 cut(s) 1367, 1391, 2486, 2842, 2848
NmeAIII GCCGAG 1 cut(s) 2840
NmuCI GTSAC 8 cut(s) 358, 468, 1972, 2138, 2620, 2794, 2935, 3473
NsiI ATGCAT 1 cut(s) 3496
NspI RCATGY 4 cut(s) 972, 1838, 2707, 3150
NspV TTCGAA 1 cut(s) 1771
OliI CACNNNNGTG 1 cut(s) 2627
PaeR7I CTCGAG 1 cut(s) 1707
PagI TCATGA 2 cut(s) 211, 3067
PaqCI CACCTGC 1 cut(s) 1498
PciI ACATGT 2 cut(s) 2703, 3146
PciSI GCTCTTC 3 cut(s) 1845, 1940, 3202
PctI GAATGC 1 cut(s) 2767
PdiI GCCGGC 1 cut(s) 3366
PdmI GAANNNNTTC 4 cut(s) 131, 219, 1299, 2352
PflFI GACNNNGTC 1 cut(s) 1469
PflMI CCANNNNNTGG 2 cut(s) 443, 1674
PfoI TCCNGGA 1 cut(s) 790
PleI GAGTC 4 cut(s) 596, 854, 1718, 3412
PpsI GAGTC 4 cut(s) 596, 854, 1718, 3412
Ppu21I YACGTR 1 cut(s) 3372
PscI ACATGT 2 cut(s) 2703, 3146
PshBI ATTAAT 3 cut(s) 693, 2202, 2667
Psp6I CCWGG 4 cut(s) 418, 790, 3166, 3279
PspFI CCCAGC 2 cut(s) 911, 952
PspGI CCWGG 4 cut(s) 418, 790, 3166, 3279
PspN4I GGNNCC 5 cut(s) 1367, 1391, 2486, 2842, 2848
PspPI GGNCC 6 cut(s) 422, 560, 1405, 1465, 2840, 2902
PspXI VCTCGAGB 1 cut(s) 1707
PstI CTGCAG 1 cut(s) 1206
PstNI CAGNNNCTG 2 cut(s) 1210, 3119
PsuI RGATCY 4 cut(s) 460, 1365, 1389, 2047
PsyI GACNNNGTC 1 cut(s) 1469
PvuII CAGCTG 1 cut(s) 1793
RsaI GTAC 4 cut(s) 747, 2486, 2875, 3485
RsaNI GTAC 4 cut(s) 746, 2485, 2874, 3484
RseI CAYNNNNRTG 6 cut(s) 1098, 1818, 2016, 2234, 2627, 3499
SapI GCTCTTC 3 cut(s) 1845, 1940, 3202
Sau96I GGNCC 6 cut(s) 422, 560, 1405, 1465, 2840, 2902
SchI GAGTC 4 cut(s) 596, 854, 1719, 3413
ScrFI CCNGG 7 cut(s) 420, 776, 792, 2500, 2844, 3168, 3281
SduI GDGCHC 3 cut(s) 1195, 2959, 3104
SfaNI GCATC 4 cut(s) 832, 1793, 2200, 2854
SfcI CTRYAG 3 cut(s) 629, 936, 1202
Sfr274I CTCGAG 1 cut(s) 1707
SfuI TTCGAA 1 cut(s) 1771
SinI GGWCC 3 cut(s) 560, 1405, 1465
SlaI CTCGAG 1 cut(s) 1707
SmiMI CAYNNNNRTG 6 cut(s) 1098, 1818, 2016, 2234, 2627, 3499
SmlI CTYRAG 4 cut(s) 1620, 1707, 2475, 2756
SmoI CTYRAG 4 cut(s) 1620, 1707, 2475, 2756
SnaBI TACGTA 1 cut(s) 3372
SsiI CCGC 3 cut(s) 238, 1400, 2916
SspI AATATT 2 cut(s) 199, 1537
SspMI CTAG 7 cut(s) 17, 275, 2259, 3024, 3123, 3318, 3356
StyD4I CCNGG 7 cut(s) 418, 774, 790, 2498, 2842, 3166, 3279
StyI CCWWGG 3 cut(s) 30, 1853, 2831
TaiI ACGT 6 cut(s) 501, 1457, 1550, 2775, 2966, 3374
TaqI TCGA 5 cut(s) 826, 1708, 1771, 2889, 3087
TatI WGTACW 1 cut(s) 745
TscAI CASTG 6 cut(s) 157, 1588, 2725, 3109, 3354, 3445
TseFI GTSAC 8 cut(s) 358, 468, 1972, 2138, 2620, 2794, 2935, 3473
Tsp45I GTSAC 8 cut(s) 358, 468, 1972, 2138, 2620, 2794, 2935, 3473
TspGWI ACGGA 1 cut(s) 3299
TspRI CASTG 6 cut(s) 157, 1588, 2725, 3109, 3354, 3445
Tth111I GACNNNGTC 1 cut(s) 1469
Van91I CCANNNNNTGG 2 cut(s) 443, 1674
Vha464I CTTAAG 1 cut(s) 2756
VpaK11BI GGWCC 3 cut(s) 560, 1405, 1465
VspI ATTAAT 3 cut(s) 693, 2202, 2667
XagI CCTNNNNNAGG 1 cut(s) 3056
XceI RCATGY 4 cut(s) 972, 1838, 2707, 3150
XhoI CTCGAG 1 cut(s) 1707
XmiI GTMKAC 2 cut(s) 2700, 3339
XmnI GAANNNNTTC 4 cut(s) 131, 219, 1299, 2352
XspI CTAG 7 cut(s) 17, 275, 2259, 3024, 3123, 3318, 3356
Zsp2I ATGCAT 1 cut(s) 3496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.