Belongs to the glyceraldehyde-3-phosphate dehydrogenase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 743 |
| AccB1I | GGYRCC | 2 cut(s) | 278, 350 |
| AccI | GTMKAC | 1 cut(s) | 299 |
| AccII | CGCG | 1 cut(s) | 332 |
| AciI | CCGC | 2 cut(s) | 330, 600 |
| AclWI | GGATC | 3 cut(s) | 98, 248, 253 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcuI | CTGAAG | 1 cut(s) | 53 |
| AcyI | GRCGYC | 1 cut(s) | 408 |
| AfaI | GTAC | 1 cut(s) | 134 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 382, 490, 781 |
| AflII | CTTAAG | 1 cut(s) | 182 |
| AflIII | ACRYGT | 1 cut(s) | 135 |
| AgsI | TTSAA | 3 cut(s) | 142, 343, 619 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 471 |
| AjiI | CACGTC | 1 cut(s) | 605 |
| AluBI | AGCT | 5 cut(s) | 88, 181, 459, 644, 674 |
| AluI | AGCT | 5 cut(s) | 88, 181, 459, 644, 674 |
| Alw21I | GWGCWC | 1 cut(s) | 90 |
| AlwI | GGATC | 3 cut(s) | 98, 248, 253 |
| AoxI | GGCC | 6 cut(s) | 3, 222, 523, 574, 699, 762 |
| ApeKI | GCWGC | 5 cut(s) | 459, 608, 633, 644, 785 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 348, 380 |
| AspS9I | GGNCC | 3 cut(s) | 161, 523, 575 |
| AsuHPI | GGTGA | 3 cut(s) | 106, 227, 281 |
| AvaII | GGWCC | 1 cut(s) | 161 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanI | GGYRCC | 2 cut(s) | 278, 350 |
| BanII | GRGCYC | 1 cut(s) | 90 |
| Bbv12I | GWGCWC | 1 cut(s) | 90 |
| BbvI | GCAGC | 5 cut(s) | 446, 595, 631, 645, 772 |
| BccI | CCATC | 3 cut(s) | 554, 566, 586 |
| BceAI | ACGGC | 1 cut(s) | 209 |
| BfaI | CTAG | 2 cut(s) | 57, 630 |
| BfrI | CTTAAG | 1 cut(s) | 182 |
| BisI | GCNGC | 6 cut(s) | 330, 460, 609, 634, 645, 786 |
| BlsI | GCNGC | 6 cut(s) | 331, 461, 610, 635, 646, 787 |
| Bme18I | GGWCC | 1 cut(s) | 161 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 471 |
| BmgBI | CACGTC | 1 cut(s) | 605 |
| BmgT120I | GGNCC | 3 cut(s) | 161, 523, 575 |
| BmiI | GGNNCC | 3 cut(s) | 248, 280, 352 |
| BmsI | GCATC | 1 cut(s) | 376 |
| BpmI | CTGGAG | 3 cut(s) | 324, 613, 660 |
| BpuEI | CTTGAG | 1 cut(s) | 773 |
| BsaBI | GATNNNNATC | 2 cut(s) | 14, 20 |
| BsaHI | GRCGYC | 1 cut(s) | 408 |
| BsaJI | CCNNGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 382, 490, 781 |
| Bse1I | ACTGG | 4 cut(s) | 307, 596, 643, 697 |
| Bse8I | GATNNNNATC | 2 cut(s) | 14, 20 |
| BseDI | CCNNGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| BseGI | GGATG | 1 cut(s) | 391 |
| BseJI | GATNNNNATC | 2 cut(s) | 14, 20 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 382, 490, 781 |
| BseNI | ACTGG | 4 cut(s) | 307, 596, 643, 697 |
| BseXI | GCAGC | 5 cut(s) | 446, 595, 631, 645, 772 |
| BsgI | GTGCAG | 1 cut(s) | 445 |
| Bsh1236I | CGCG | 1 cut(s) | 332 |
| BshFI | GGCC | 6 cut(s) | 5, 224, 525, 576, 701, 764 |
| BshNI | GGYRCC | 2 cut(s) | 278, 350 |
| BsiHKAI | GWGCWC | 1 cut(s) | 90 |
| BslFI | GGGAC | 1 cut(s) | 89 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 382, 490, 781 |
| BsmFI | GGGAC | 1 cut(s) | 89 |
| BsnI | GGCC | 6 cut(s) | 5, 224, 525, 576, 701, 764 |
| Bsp1286I | GDGCHC | 1 cut(s) | 90 |
| Bsp143I | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| Bsp19I | CCATGG | 3 cut(s) | 157, 164, 263 |
| BspACI | CCGC | 2 cut(s) | 330, 600 |
| BspANI | GGCC | 6 cut(s) | 5, 224, 525, 576, 701, 764 |
| BspFNI | CGCG | 1 cut(s) | 332 |
| BspLI | GGNNCC | 3 cut(s) | 248, 280, 352 |
| BspPI | GGATC | 3 cut(s) | 98, 248, 253 |
| BspQI | GCTCTTC | 1 cut(s) | 72 |
| BspT107I | GGYRCC | 2 cut(s) | 278, 350 |
| BspTI | CTTAAG | 1 cut(s) | 182 |
| BsrI | ACTGG | 4 cut(s) | 307, 596, 643, 697 |
| BssECI | CCNNGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| BssMI | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| BssNI | GRCGYC | 1 cut(s) | 408 |
| BssT1I | CCWWGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| Bst4CI | ACNGT | 8 cut(s) | 29, 154, 232, 473, 538, 568, 718, 745 |
| Bst6I | CTCTTC | 3 cut(s) | 72, 215, 249 |
| BstACI | GRCGYC | 1 cut(s) | 408 |
| BstAFI | CTTAAG | 1 cut(s) | 182 |
| BstC8I | GCNNGC | 1 cut(s) | 457 |
| BstDSI | CCRYGG | 3 cut(s) | 157, 164, 263 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 488 |
| BstF5I | GGATG | 1 cut(s) | 391 |
| BstFNI | CGCG | 1 cut(s) | 332 |
| BstKTI | GATC | 5 cut(s) | 12, 18, 106, 243, 261 |
| BstMBI | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 62, 178, 485, 650 |
| BstNSI | RCATGY | 1 cut(s) | 139 |
| BstUI | CGCG | 1 cut(s) | 332 |
| BstV1I | GCAGC | 5 cut(s) | 446, 595, 631, 645, 772 |
| BstX2I | RGATCY | 1 cut(s) | 258 |
| BstYI | RGATCY | 1 cut(s) | 258 |
| BsuRI | GGCC | 6 cut(s) | 5, 224, 525, 576, 701, 764 |
| BtgI | CCRYGG | 3 cut(s) | 157, 164, 263 |
| BtrI | CACGTC | 1 cut(s) | 605 |
| BtsCI | GGATG | 1 cut(s) | 391 |
| BtsIMutI | CAGTG | 7 cut(s) | 150, 228, 312, 534, 636, 714, 741 |
| Cac8I | GCNNGC | 1 cut(s) | 457 |
| Cfr13I | GGNCC | 3 cut(s) | 161, 523, 575 |
| CseI | GACGC | 1 cut(s) | 397 |
| Csp6I | GTAC | 1 cut(s) | 133 |
| CviAII | CATG | 7 cut(s) | 127, 136, 158, 165, 176, 264, 394 |
| CviQI | GTAC | 1 cut(s) | 133 |
| DpnI | GATC | 5 cut(s) | 11, 17, 105, 242, 260 |
| DpnII | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 743 |
| DriI | GACNNNNNGTC | 1 cut(s) | 471 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 743 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 3 cut(s) | 72, 215, 249 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 471 |
| EarI | CTCTTC | 3 cut(s) | 72, 215, 249 |
| EciI | GGCGGA | 1 cut(s) | 615 |
| Ecl136II | GAGCTC | 1 cut(s) | 88 |
| Eco130I | CCWWGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| Eco24I | GRGCYC | 1 cut(s) | 90 |
| Eco47I | GGWCC | 1 cut(s) | 161 |
| Eco53kI | GAGCTC | 1 cut(s) | 88 |
| Eco57I | CTGAAG | 1 cut(s) | 53 |
| EcoICRI | GAGCTC | 1 cut(s) | 88 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 488 |
| EcoO109I | RGGNCCY | 1 cut(s) | 523 |
| EcoT14I | CCWWGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| EcoT38I | GRGCYC | 1 cut(s) | 90 |
| ErhI | CCWWGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| FaeI | CATG | 7 cut(s) | 130, 139, 161, 168, 179, 267, 397 |
| FaqI | GGGAC | 1 cut(s) | 89 |
| FatI | CATG | 7 cut(s) | 126, 135, 157, 164, 175, 263, 393 |
| FauNDI | CATATG | 1 cut(s) | 769 |
| FblI | GTMKAC | 1 cut(s) | 299 |
| Fnu4HI | GCNGC | 6 cut(s) | 330, 460, 609, 634, 645, 786 |
| FokI | GGATG | 1 cut(s) | 398 |
| FriOI | GRGCYC | 1 cut(s) | 90 |
| Fsp4HI | GCNGC | 6 cut(s) | 330, 460, 609, 634, 645, 786 |
| FspBI | CTAG | 2 cut(s) | 57, 630 |
| GluI | GCNGC | 6 cut(s) | 330, 460, 609, 634, 645, 786 |
| GsuI | CTGGAG | 3 cut(s) | 324, 613, 660 |
| HaeIII | GGCC | 6 cut(s) | 5, 224, 525, 576, 701, 764 |
| HgaI | GACGC | 1 cut(s) | 397 |
| Hin1I | GRCGYC | 1 cut(s) | 408 |
| Hin1II | CATG | 7 cut(s) | 130, 139, 161, 168, 179, 267, 397 |
| HincII | GTYRAC | 2 cut(s) | 100, 736 |
| HindII | GTYRAC | 2 cut(s) | 100, 736 |
| HinfI | GANTC | 4 cut(s) | 21, 296, 334, 507 |
| HpaI | GTTAAC | 1 cut(s) | 100 |
| HphI | GGTGA | 3 cut(s) | 106, 227, 281 |
| Hpy166II | GTNNAC | 5 cut(s) | 100, 300, 312, 541, 736 |
| Hpy188I | TCNGA | 5 cut(s) | 20, 35, 72, 433, 512 |
| Hpy188III | TCNNGA | 3 cut(s) | 13, 238, 244 |
| Hpy8I | GTNNAC | 5 cut(s) | 100, 300, 312, 541, 736 |
| Hpy99I | CGWCG | 1 cut(s) | 95 |
| HpyAV | CCTTC | 7 cut(s) | 30, 214, 215, 337, 412, 712, 754 |
| HpyCH4III | ACNGT | 8 cut(s) | 29, 154, 232, 473, 538, 568, 718, 745 |
| HpyCH4IV | ACGT | 2 cut(s) | 131, 604 |
| HpyCH4V | TGCA | 1 cut(s) | 462 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 62, 178, 485, 650 |
| HpySE526I | ACGT | 2 cut(s) | 131, 604 |
| Hsp92I | GRCGYC | 1 cut(s) | 408 |
| Hsp92II | CATG | 7 cut(s) | 130, 139, 161, 168, 179, 267, 397 |
| KspAI | GTTAAC | 1 cut(s) | 100 |
| Kzo9I | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| LguI | GCTCTTC | 1 cut(s) | 72 |
| LmnI | GCTCC | 2 cut(s) | 484, 641 |
| Lsp1109I | GCAGC | 5 cut(s) | 446, 595, 631, 645, 772 |
| LweI | GCATC | 1 cut(s) | 376 |
| MaeI | CTAG | 2 cut(s) | 57, 630 |
| MaeII | ACGT | 2 cut(s) | 131, 604 |
| MaeIII | GTNAC | 1 cut(s) | 226 |
| MalI | GATC | 5 cut(s) | 11, 17, 105, 242, 260 |
| MboI | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| MboII | GAAGA | 4 cut(s) | 59, 202, 266, 769 |
| MflI | RGATCY | 1 cut(s) | 258 |
| MhlI | GDGCHC | 1 cut(s) | 90 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 2 cut(s) | 513, 686 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 2 cut(s) | 305, 328 |
| MmeI | TCCRAC | 1 cut(s) | 637 |
| MnlI | CCTC | 5 cut(s) | 66, 265, 514, 590, 749 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 3 cut(s) | 99, 183, 780 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspA1I | CMGCKG | 1 cut(s) | 459 |
| MspCI | CTTAAG | 1 cut(s) | 182 |
| MvnI | CGCG | 1 cut(s) | 332 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 62, 178, 485, 650 |
| NcoI | CCATGG | 3 cut(s) | 157, 164, 263 |
| NdeI | CATATG | 1 cut(s) | 769 |
| NdeII | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| NlaIII | CATG | 7 cut(s) | 130, 139, 161, 168, 179, 267, 397 |
| NlaIV | GGNNCC | 3 cut(s) | 248, 280, 352 |
| NmuCI | GTSAC | 1 cut(s) | 226 |
| NspI | RCATGY | 1 cut(s) | 139 |
| PciI | ACATGT | 1 cut(s) | 135 |
| PciSI | GCTCTTC | 1 cut(s) | 72 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 99 |
| PfeI | GAWTC | 2 cut(s) | 21, 507 |
| PflFI | GACNNNGTC | 1 cut(s) | 152 |
| PkrI | GCNGC | 6 cut(s) | 331, 461, 610, 635, 646, 787 |
| PleI | GAGTC | 2 cut(s) | 304, 328 |
| PpsI | GAGTC | 2 cut(s) | 304, 328 |
| PscI | ACATGT | 1 cut(s) | 135 |
| Psp124BI | GAGCTC | 1 cut(s) | 90 |
| PspN4I | GGNNCC | 3 cut(s) | 248, 280, 352 |
| PspPI | GGNCC | 3 cut(s) | 161, 523, 575 |
| PsuI | RGATCY | 1 cut(s) | 258 |
| PsyI | GACNNNGTC | 1 cut(s) | 152 |
| PvuII | CAGCTG | 1 cut(s) | 459 |
| RsaI | GTAC | 1 cut(s) | 134 |
| RsaNI | GTAC | 1 cut(s) | 133 |
| SacI | GAGCTC | 1 cut(s) | 90 |
| SapI | GCTCTTC | 1 cut(s) | 72 |
| SaqAI | TTAA | 3 cut(s) | 99, 183, 780 |
| SatI | GCNGC | 6 cut(s) | 330, 460, 609, 634, 645, 786 |
| Sau3AI | GATC | 5 cut(s) | 9, 15, 103, 240, 258 |
| Sau96I | GGNCC | 3 cut(s) | 161, 523, 575 |
| SchI | GAGTC | 2 cut(s) | 305, 328 |
| SduI | GDGCHC | 1 cut(s) | 90 |
| SfaNI | GCATC | 1 cut(s) | 376 |
| SinI | GGWCC | 1 cut(s) | 161 |
| SmlI | CTYRAG | 2 cut(s) | 182, 752 |
| SmoI | CTYRAG | 2 cut(s) | 182, 752 |
| Sse9I | AATT | 2 cut(s) | 513, 686 |
| SsiI | CCGC | 2 cut(s) | 330, 600 |
| SspMI | CTAG | 2 cut(s) | 57, 630 |
| SstI | GAGCTC | 1 cut(s) | 90 |
| StyI | CCWWGG | 5 cut(s) | 157, 164, 263, 489, 648 |
| TaaI | ACNGT | 8 cut(s) | 29, 154, 232, 473, 538, 568, 718, 745 |
| TaiI | ACGT | 2 cut(s) | 134, 607 |
| TasI | AATT | 2 cut(s) | 513, 686 |
| TauI | GCSGC | 1 cut(s) | 332 |
| TfiI | GAWTC | 2 cut(s) | 21, 507 |
| Tru1I | TTAA | 3 cut(s) | 99, 183, 780 |
| Tru9I | TTAA | 3 cut(s) | 99, 183, 780 |
| TscAI | CASTG | 7 cut(s) | 157, 235, 319, 541, 643, 721, 748 |
| TseFI | GTSAC | 1 cut(s) | 226 |
| TseI | GCWGC | 5 cut(s) | 459, 608, 633, 644, 785 |
| Tsp45I | GTSAC | 1 cut(s) | 226 |
| TspDTI | ATGAA | 2 cut(s) | 100, 603 |
| TspGWI | ACGGA | 1 cut(s) | 695 |
| TspRI | CASTG | 7 cut(s) | 157, 235, 319, 541, 643, 721, 748 |
| Tth111I | GACNNNGTC | 1 cut(s) | 152 |
| Vha464I | CTTAAG | 1 cut(s) | 182 |
| VpaK11BI | GGWCC | 1 cut(s) | 161 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 488 |
| XceI | RCATGY | 1 cut(s) | 139 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 400 |
| XmiI | GTMKAC | 1 cut(s) | 299 |
| XspI | CTAG | 2 cut(s) | 57, 630 |