pycom08g05620

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001406
Physical Location & Seq
Reverse (-)
4263634 .. 4264047
414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05620.1

Sequence Viewer

Length: 324 bp
ATGTCACGGATAAAATATGGTCACACTGTTCTTGATTTTGGGTGTGGCTGGGAATCTTTGTCATTGTGCATTGCACAGAGATATAGCAACTGCAAAGTTACCGGGATCTGCAGCTTGACGACACAAAAAGCTTCCGTGGAAGAGCAATGCCTGTGCGTAACTGCAGAATGTAGAGATGTCATCGGAGATATCAGCACGTTTGAAACAGAGGCTTCCTGCTTCATAAAATCAATTATGGATTCAGCCGTGAAATGGACAGTATATTGGCCTACATTCTTCATTTCGGTGGCAATGCCAAAGCTCTGTTTGCGTACGGAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

108

Amino Acids

12.01

Weight (kDa)

5.74

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 113
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 252
AgsI TTSAA 1 cut(s) 203
AluBI AGCT 3 cut(s) 114, 131, 301
AluI AGCT 3 cut(s) 114, 131, 301
AlwI GGATC 1 cut(s) 113
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 103
BbvI GCAGC 1 cut(s) 123
BceAI ACGGC 1 cut(s) 230
BcnI CCSGG 1 cut(s) 103
BfmI CTRYAG 2 cut(s) 109, 162
BisI GCNGC 1 cut(s) 112
BlsI GCNGC 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse3DI GCAATG 3 cut(s) 69, 152, 297
BseDI CCNNGG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMI GCAATG 3 cut(s) 69, 152, 297
BseXI GCAGC 1 cut(s) 123
BseYI CCCAGC 1 cut(s) 48
BshFI GGCC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 102
BsiWI CGTACG 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 252
BsnI GGCC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 105
BspANI GGCC 1 cut(s) 268
BspMAI CTGCAG 2 cut(s) 113, 166
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 135
BsrDI GCAATG 3 cut(s) 69, 152, 297
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 28, 259
Bst6I CTCTTC 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 135
BstKTI GATC 1 cut(s) 108
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 307, 316
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 2 cut(s) 109, 162
BstV1I GCAGC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 1 cut(s) 268
BtgI CCRYGG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 24
Csp6I GTAC 1 cut(s) 312
CviJI RGCY 7 cut(s) 48, 114, 131, 212, 245, 268, 301
CviKI_1 RGCY 7 cut(s) 48, 114, 131, 212, 245, 268, 301
CviQI GTAC 1 cut(s) 312
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 190
EcoRV GATATC 1 cut(s) 190
FaiI YATR 5 cut(s) 18, 84, 224, 236, 262
Fnu4HI GCNGC 1 cut(s) 112
Fsp4HI GCNGC 1 cut(s) 112
GluI GCNGC 1 cut(s) 112
GsaI CCCAGC 1 cut(s) 52
HaeIII GGCC 1 cut(s) 268
HapII CCGG 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 2 cut(s) 53, 239
HpaII CCGG 1 cut(s) 102
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 1 cut(s) 32
HpyCH4III ACNGT 2 cut(s) 28, 259
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 5 cut(s) 69, 74, 93, 111, 164
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 316
HpySE526I ACGT 1 cut(s) 197
Kzo9I GATC 1 cut(s) 105
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 4 cut(s) 34, 115, 164, 229
Lsp1109I GCAGC 1 cut(s) 123
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 4 cut(s) 3, 20, 97, 157
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 2 cut(s) 152, 268
MflI RGATCY 1 cut(s) 105
MluCI AATT 1 cut(s) 231
MnlI CCTC 1 cut(s) 202
MslI CAYNNNNRTG 1 cut(s) 284
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 307, 316
NciI CCSGG 1 cut(s) 103
NdeII GATC 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 3, 20
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 53, 239
Pfl23II CGTACG 1 cut(s) 311
PkrI GCNGC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 48
PspLI CGTACG 1 cut(s) 311
PstI CTGCAG 2 cut(s) 113, 166
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
RseI CAYNNNNRTG 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 103
SetI ASST 4 cut(s) 116, 133, 200, 303
SfcI CTRYAG 2 cut(s) 109, 162
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 101
TaaI ACNGT 2 cut(s) 28, 259
TaiI ACGT 1 cut(s) 200
TasI AATT 1 cut(s) 231
TfiI GAWTC 2 cut(s) 53, 239
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 2 cut(s) 3, 20
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 2 cut(s) 3, 20
TspDTI ATGAA 2 cut(s) 211, 268
TspGWI ACGGA 2 cut(s) 22, 124
TspRI CASTG 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.