RLG00000014204

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001406
Physical Location & Seq
Reverse (-)
49292650 .. 49293287
638 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014204

Sequence Viewer

Length: 549 bp
ATGGCCCAAATCGGACAGATCTGGTTTGTGGCTGACCATGGGTTGGTGATCCGTGAACGACCAAATGGGCGATTTCTCTTCCGCTTTGTTAATCCGACGGATAAGCAACGGGTTTTGGCTAAATCACCGTGGTATTATCGTTCGGCAACGTTGGTTTTAGGGGACTATGATGGGGTGGGTGAGATCATGGAGGTGCCTCTGAATCGATTGCCGATTTGGATCCAGATCAGAGGATTGCTGCAGGCCTATTCCACACCGAGTTGGGCAAGGAAGGTTGCTGGGTGCTTGGGTCGGGTTGTTCATGTGGATGAAAGGAGATTGAAGCGGCATGAGGAAGTGAAGGGTGATTCATCGGTGGTGGTGGAGGCTGTTCCCCGTCCGAGTGGGATGGGAGTACCACCGGGTTTTGAAGCTTTACCGGGATCTAAGGGGGTGGGAGACCATCCTGGGATTGGGCCATTGATGTTGGATGGGAAGATAGATTCTCGGGCCATTGATGTTGGAAGGGAAGATAGATTCTCGGCCGCTGGTGTTGGAGGAGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

183

Amino Acids

19.97

Weight (kDa)

9.0

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 11 - 53 1.6e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 43
AciI CCGC 3 cut(s) 82, 325, 525
AclI AACGTT 1 cut(s) 149
AclWI GGATC 4 cut(s) 43, 214, 227, 430
AcoI YGGCCR 1 cut(s) 522
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 43, 452
AgsI TTSAA 2 cut(s) 322, 410
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 1 cut(s) 413
AluI AGCT 1 cut(s) 413
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 4 cut(s) 43, 214, 227, 430
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 5 cut(s) 3, 243, 455, 489, 522
ApeKI GCWGC 1 cut(s) 238
AspS9I GGNCC 3 cut(s) 4, 455, 489
AsuC2I CCSGG 2 cut(s) 402, 420
AsuHPI GGTGA 4 cut(s) 58, 117, 191, 356
AvaI CYCGRG 1 cut(s) 486
BamHI GGATCC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 193
BbvI GCAGC 1 cut(s) 225
BccI CCATC 4 cut(s) 164, 382, 450, 464
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 2 cut(s) 402, 420
BcoDI GTCTC 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 239
BglII AGATCT 1 cut(s) 18
BisI GCNGC 3 cut(s) 239, 326, 525
BlsI GCNGC 3 cut(s) 240, 327, 526
Bme1390I CCNGG 3 cut(s) 402, 420, 447
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 4, 455, 489
BmiI GGNNCC 2 cut(s) 195, 221
BmrFI CCNGG 3 cut(s) 402, 420, 447
BpuMI CCSGG 2 cut(s) 402, 420
Bsa29I ATCGAT 1 cut(s) 205
BsaBI GATNNNNATC 2 cut(s) 218, 224
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 3 cut(s) 37, 128, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 452
Bse8I GATNNNNATC 2 cut(s) 218, 224
BseBI CCWGG 1 cut(s) 447
BseCI ATCGAT 1 cut(s) 205
BseDI CCNNGG 3 cut(s) 37, 128, 446
BseGI GGATG 4 cut(s) 313, 393, 442, 475
BseJI GATNNNNATC 2 cut(s) 218, 224
BseLI CCNNNNNNNGG 2 cut(s) 43, 452
BseX3I CGGCCG 1 cut(s) 522
BseXI GCAGC 1 cut(s) 225
BseYI CCCAGC 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 525
BshFI GGCC 5 cut(s) 5, 245, 457, 491, 524
BshNI GGYRCC 1 cut(s) 193
BshVI ATCGAT 1 cut(s) 205
BsiEI CGRYCG 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 2 cut(s) 401, 419
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 43, 452
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 5 cut(s) 5, 245, 457, 491, 524
Bso31I GGTCTC 1 cut(s) 432
BsoBI CYCGRG 1 cut(s) 486
Bsp143I GATC 6 cut(s) 18, 48, 183, 219, 225, 422
Bsp19I CCATGG 1 cut(s) 37
BspACI CCGC 3 cut(s) 82, 325, 525
BspANI GGCC 5 cut(s) 5, 245, 457, 491, 524
BspDI ATCGAT 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 195, 221
BspMAI CTGCAG 1 cut(s) 243
BspPI GGATC 4 cut(s) 43, 214, 227, 430
BspT107I GGYRCC 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 37, 128, 446
BssMI GATC 6 cut(s) 18, 48, 183, 219, 225, 422
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 2 cut(s) 37, 128
BstF5I GGATG 4 cut(s) 313, 393, 442, 475
BstKTI GATC 6 cut(s) 21, 51, 186, 222, 228, 425
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 6 cut(s) 18, 48, 183, 219, 225, 422
BstMCI CGRYCG 1 cut(s) 525
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 400, 418, 445
BstSFI CTRYAG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 3 cut(s) 18, 219, 422
BstYI RGATCY 3 cut(s) 18, 219, 422
BstZI CGGCCG 1 cut(s) 522
Bsu15I ATCGAT 1 cut(s) 205
BsuRI GGCC 5 cut(s) 5, 245, 457, 491, 524
BsuTUI ATCGAT 1 cut(s) 205
BtgI CCRYGG 2 cut(s) 37, 128
BtsCI GGATG 4 cut(s) 313, 393, 442, 475
Cac8I GCNNGC 1 cut(s) 243
Cfr13I GGNCC 3 cut(s) 4, 455, 489
ClaI ATCGAT 1 cut(s) 205
Csp6I GTAC 1 cut(s) 395
CviAII CATG 4 cut(s) 38, 187, 302, 329
CviJI RGCY 9 cut(s) 5, 32, 119, 245, 368, 413, 457, 491, 524
CviKI_1 RGCY 9 cut(s) 5, 32, 119, 245, 368, 413, 457, 491, 524
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 1 cut(s) 426
DpnI GATC 6 cut(s) 20, 50, 185, 221, 227, 424
DpnII GATC 6 cut(s) 18, 48, 183, 219, 225, 422
EaeI YGGCCR 1 cut(s) 522
EagI CGGCCG 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 37
Eco147I AGGCCT 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 432
Eco52I CGGCCG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 4 cut(s) 41, 190, 305, 332
FaiI YATR 5 cut(s) 39, 168, 188, 303, 330
FaqI GGGAC 1 cut(s) 176
FatI CATG 4 cut(s) 37, 186, 301, 328
Fnu4HI GCNGC 3 cut(s) 239, 326, 525
FokI GGATG 4 cut(s) 320, 400, 429, 482
Fsp4HI GCNGC 3 cut(s) 239, 326, 525
GluI GCNGC 3 cut(s) 239, 326, 525
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 5 cut(s) 5, 245, 457, 491, 524
HapII CCGG 2 cut(s) 401, 419
Hin1II CATG 4 cut(s) 41, 190, 305, 332
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 4 cut(s) 202, 347, 482, 516
HpaII CCGG 2 cut(s) 401, 419
HphI GGTGA 4 cut(s) 58, 117, 191, 356
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 5 cut(s) 14, 96, 201, 230, 381
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 56
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 265, 334, 498
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 4 cut(s) 41, 190, 305, 332
Kzo9I GATC 6 cut(s) 18, 48, 183, 219, 225, 422
LpnPI CCDG 9 cut(s) 7, 227, 236, 264, 414, 432, 432, 459, 513
Lsp1109I GCAGC 1 cut(s) 225
MaeII ACGT 1 cut(s) 149
MalI GATC 6 cut(s) 20, 50, 185, 221, 227, 424
MboI GATC 6 cut(s) 18, 48, 183, 219, 225, 422
MboII GAAGA 3 cut(s) 70, 487, 521
MflI RGATCY 3 cut(s) 18, 219, 422
MmeI TCCRAC 4 cut(s) 119, 447, 481, 514
MnlI CCTC 6 cut(s) 184, 207, 224, 325, 358, 530
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 2 cut(s) 191, 306
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 2 cut(s) 401, 419
MspR9I CCNGG 3 cut(s) 402, 420, 447
MvaI CCWGG 1 cut(s) 447
NciI CCSGG 2 cut(s) 402, 420
NcoI CCATGG 1 cut(s) 37
NdeII GATC 6 cut(s) 18, 48, 183, 219, 225, 422
NlaIII CATG 4 cut(s) 41, 190, 305, 332
NlaIV GGNNCC 2 cut(s) 195, 221
NmeAIII GCCGAG 1 cut(s) 500
PceI AGGCCT 1 cut(s) 245
PfeI GAWTC 4 cut(s) 202, 347, 482, 516
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 3 cut(s) 240, 327, 526
Psp1406I AACGTT 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 195, 221
PspPI GGNCC 3 cut(s) 4, 455, 489
PstI CTGCAG 1 cut(s) 243
PsuI RGATCY 3 cut(s) 18, 219, 422
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
RseI CAYNNNNRTG 2 cut(s) 191, 306
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 3 cut(s) 239, 326, 525
Sau3AI GATC 6 cut(s) 18, 48, 183, 219, 225, 422
Sau96I GGNCC 3 cut(s) 4, 455, 489
ScrFI CCNGG 3 cut(s) 402, 420, 447
SetI ASST 4 cut(s) 152, 195, 276, 415
SfcI CTRYAG 1 cut(s) 239
SmiMI CAYNNNNRTG 2 cut(s) 191, 306
SseBI AGGCCT 1 cut(s) 245
SsiI CCGC 3 cut(s) 82, 325, 525
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 3 cut(s) 400, 418, 445
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 205
TauI GCSGC 2 cut(s) 328, 527
TfiI GAWTC 4 cut(s) 202, 347, 482, 516
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseI GCWGC 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 290, 324, 339
TspGWI ACGGA 2 cut(s) 41, 113
Van91I CCANNNNNTGG 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.