Rroxscaffold_3G00237240

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001406
Physical Location & Seq
Forward (+)
24860580 .. 24867081
6502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237240.1

Sequence Viewer

Length: 708 bp
ATGAGGTGGCGGAGTTGTGTTTTCGGCCGGGTCCGAAAACGCAAGCTGCACGCCTTGATGCACCACCGTGGAAGAGAATCGGCGCTAGAGGGCACTACGGGGACGACCGAAGGAGGAGGCGACCTGGCGACCAAGTTTCACGGCGGAGACCGCGCGATTGGCGGAAATGTCGAGTCGGATCGACCGGGTCCCGGGTCGGGGAGATTGGGATCTGGTGTTGATCAAGGGCCTAATGACTCACCTGGAGCTTACGTGGGTGATGTGCAATTGGCTCCTATTAGCCCAGATTCCTTGGGTCATCGGAAGCGCAGGTGCTGCTTCAACTTTCGGTTTTGGGATGGAGACCTCCCTACCAAAGGCTTGCAGTTTGAGGTGGGGAAGTCTTCTTCCCCTACTACTGGTAAGGTAGGGGCTGGGAAGAAACATAAACCAAAGATTCTCTCGGCTGCAAAGCATCGTGCCTTGCTCTCTAGGCTTGAGGAGGAGATTAAAGATGGCCATACTGTTCTTGATGTTGGATGTGGATGGGGATCACCATCTCTGTACATTGCACAGAAATATAAGAATTGCAGGGTTACTGGGATCTGCATTTCAGCAACACATAAGGCTTTTATAGAAGAGCAGTGTAGGAATTGTCAGCTGGAGAATGTGGAGATCATCGTTGCAGATATCAGTACAATATTTGAGATAGAGGCATCATATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

235

Amino Acids

25.5

Weight (kDa)

9.02

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 150 - 221 2.1e-10 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 163 - 229 7e-07 Methyltransferase domain
Methyltransf_25 PF13649 169 - 235 3.8e-09 Methyltransferase domain
Methyltransf_11 PF08241 170 - 228 4.1e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 300
AccII CGCG 2 cut(s) 153, 155
AciI CCGC 4 cut(s) 10, 144, 151, 162
AclWI GGATC 4 cut(s) 186, 217, 538, 590
AcoI YGGCCR 2 cut(s) 25, 496
AfaI GTAC 2 cut(s) 545, 676
AfiI CCNNNNNNNGG 5 cut(s) 191, 197, 198, 356, 398
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 2 cut(s) 123, 241
AleI CACNNNNGTG 1 cut(s) 66
AluBI AGCT 3 cut(s) 46, 248, 640
AluI AGCT 3 cut(s) 46, 248, 640
Alw26I GTCTC 2 cut(s) 141, 336
AlwI GGATC 4 cut(s) 186, 217, 538, 590
AlwNI CAGNNNCTG 1 cut(s) 315
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 3 cut(s) 25, 227, 496
ApeKI GCWGC 3 cut(s) 46, 315, 446
AspLEI GCGC 3 cut(s) 85, 155, 309
AspS9I GGNCC 3 cut(s) 31, 188, 227
AsuC2I CCSGG 4 cut(s) 29, 186, 192, 193
AsuHPI GGTGA 3 cut(s) 231, 269, 525
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 2 cut(s) 31, 188
BaeGI GKGCMC 1 cut(s) 95
BalI TGGCCA 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 33, 302, 433
BccI CCATC 4 cut(s) 332, 488, 519, 544
BceAI ACGGC 1 cut(s) 157
BciT130I CCWGG 2 cut(s) 125, 243
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 4 cut(s) 29, 186, 192, 193
BcoDI GTCTC 2 cut(s) 141, 336
BfaI CTAG 2 cut(s) 86, 471
BfoI RGCGCY 1 cut(s) 86
BfuAI ACCTGC 1 cut(s) 300
BisI GCNGC 3 cut(s) 47, 316, 447
BlsI GCNGC 3 cut(s) 48, 317, 448
Bme1390I CCNGG 6 cut(s) 29, 125, 186, 192, 193, 243
Bme18I GGWCC 2 cut(s) 31, 188
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 3 cut(s) 31, 188, 227
BmiI GGNNCC 4 cut(s) 32, 189, 190, 273
BmrFI CCNGG 6 cut(s) 29, 125, 186, 192, 193, 243
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 3 cut(s) 48, 463, 704
BmuI ACTGGG 1 cut(s) 588
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 264, 662
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 4 cut(s) 29, 186, 192, 193
BsaAI YACGTR 1 cut(s) 253
BsaBI GATNNNNATC 3 cut(s) 208, 529, 535
BsaI GGTCTC 2 cut(s) 141, 336
BsaJI CCNNGG 3 cut(s) 67, 191, 291
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 197, 198, 356, 398
Bse1I ACTGG 2 cut(s) 403, 583
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 3 cut(s) 208, 529, 535
BseBI CCWGG 2 cut(s) 125, 243
BseDI CCNNGG 3 cut(s) 67, 191, 291
BseGI GGATG 3 cut(s) 343, 524, 530
BseJI GATNNNNATC 3 cut(s) 208, 529, 535
BseLI CCNNNNNNNGG 5 cut(s) 191, 197, 198, 356, 398
BseMI GCAATG 1 cut(s) 546
BseNI ACTGG 2 cut(s) 403, 583
BseRI GAGGAG 3 cut(s) 129, 494, 497
BseSI GKGCMC 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 33, 302, 433
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 32
Bsh1236I CGCG 2 cut(s) 153, 155
Bsh1285I CGRYCG 3 cut(s) 28, 108, 185
BshFI GGCC 3 cut(s) 27, 229, 498
BsiEI CGRYCG 3 cut(s) 28, 108, 185
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 3 cut(s) 28, 185, 192
BslFI GGGAC 2 cut(s) 115, 174
BslI CCNNNNNNNGG 5 cut(s) 191, 197, 198, 356, 398
BsmAI GTCTC 2 cut(s) 141, 336
BsmFI GGGAC 2 cut(s) 115, 174
BsnI GGCC 3 cut(s) 27, 229, 498
Bso31I GGTCTC 2 cut(s) 141, 336
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 6 cut(s) 178, 209, 220, 530, 582, 654
BspACI CCGC 4 cut(s) 10, 144, 151, 162
BspANI GGCC 3 cut(s) 27, 229, 498
BspFNI CGCG 2 cut(s) 153, 155
BspLI GGNNCC 4 cut(s) 32, 189, 190, 273
BspMI ACCTGC 1 cut(s) 300
BspPI GGATC 4 cut(s) 186, 217, 538, 590
BspQI GCTCTTC 1 cut(s) 612
BspTNI GGTCTC 2 cut(s) 141, 336
BsrDI GCAATG 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 2 cut(s) 403, 583
BssECI CCNNGG 3 cut(s) 67, 191, 291
BssMI GATC 6 cut(s) 178, 209, 220, 530, 582, 654
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 125, 243
Bst4CI ACNGT 2 cut(s) 68, 505
Bst6I CTCTTC 2 cut(s) 67, 612
BstAPI GCANNNNNTGC 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 44, 51, 362
BstDSI CCRYGG 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 3 cut(s) 343, 524, 530
BstFNI CGCG 2 cut(s) 153, 155
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 3 cut(s) 85, 155, 309
BstKTI GATC 6 cut(s) 181, 212, 223, 533, 585, 657
BstMAI GTCTC 2 cut(s) 141, 336
BstMBI GATC 6 cut(s) 178, 209, 220, 530, 582, 654
BstMCI CGRYCG 3 cut(s) 28, 108, 185
BstMWI GCNNNNNNNGC 4 cut(s) 150, 159, 315, 472
BstNI CCWGG 2 cut(s) 125, 243
BstSCI CCNGG 6 cut(s) 27, 123, 184, 190, 191, 241
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 2 cut(s) 153, 155
BstV1I GCAGC 3 cut(s) 33, 302, 433
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 209, 582
BstYI RGATCY 2 cut(s) 209, 582
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 3 cut(s) 27, 229, 498
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 343, 524, 530
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 1 cut(s) 629
BveI ACCTGC 1 cut(s) 300
Cac8I GCNNGC 3 cut(s) 44, 51, 362
CaiI CAGNNNCTG 1 cut(s) 315
CfoI GCGC 3 cut(s) 85, 155, 309
Cfr13I GGNCC 3 cut(s) 31, 188, 227
Cfr9I CCCGGG 1 cut(s) 191
Csp6I GTAC 2 cut(s) 544, 675
CviQI GTAC 2 cut(s) 544, 675
DpnI GATC 6 cut(s) 180, 211, 222, 532, 584, 656
DpnII GATC 6 cut(s) 178, 209, 220, 530, 582, 654
EaeI YGGCCR 2 cut(s) 25, 496
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 2 cut(s) 67, 612
EarI CTCTTC 2 cut(s) 67, 612
EciI GGCGGA 3 cut(s) 25, 159, 177
EclXI CGGCCG 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 141, 336
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 2 cut(s) 31, 188
Eco52I CGGCCG 1 cut(s) 25
Eco88I CYCGRG 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 2 cut(s) 188, 227
EcoRII CCWGG 2 cut(s) 123, 241
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
FaiI YATR 7 cut(s) 426, 501, 561, 603, 614, 700, 702
FaqI GGGAC 2 cut(s) 115, 174
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 47, 316, 447
FokI GGATG 3 cut(s) 350, 531, 537
Fsp4HI GCNGC 3 cut(s) 47, 316, 447
FspBI CTAG 2 cut(s) 86, 471
GlaI GCGC 3 cut(s) 84, 154, 308
GluI GCNGC 3 cut(s) 47, 316, 447
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 2 cut(s) 264, 662
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 27, 229, 498
HapII CCGG 3 cut(s) 28, 185, 192
HhaI GCGC 3 cut(s) 85, 155, 309
Hin6I GCGC 3 cut(s) 83, 153, 307
HinP1I GCGC 3 cut(s) 83, 153, 307
HinfI GANTC 5 cut(s) 77, 173, 236, 287, 436
HpaII CCGG 3 cut(s) 28, 185, 192
HphI GGTGA 3 cut(s) 231, 269, 525
Hpy188I TCNGA 3 cut(s) 35, 178, 303
Hpy188III TCNNGA 1 cut(s) 509
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 68, 505
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 9 cut(s) 49, 61, 265, 364, 449, 551, 570, 588, 665
HpyF10VI GCNNNNNNNGC 4 cut(s) 150, 159, 315, 472
HpySE526I ACGT 1 cut(s) 252
HspAI GCGC 3 cut(s) 83, 153, 307
KflI GGGWCCC 1 cut(s) 188
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 6 cut(s) 178, 209, 220, 530, 582, 654
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 2 cut(s) 245, 277
Lsp1109I GCAGC 3 cut(s) 33, 302, 433
LweI GCATC 3 cut(s) 48, 463, 704
MaeI CTAG 2 cut(s) 86, 471
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 574
MalI GATC 6 cut(s) 180, 211, 222, 532, 584, 656
MboI GATC 6 cut(s) 178, 209, 220, 530, 582, 654
MboII GAAGA 5 cut(s) 84, 375, 378, 430, 629
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 2 cut(s) 209, 582
MhlI GDGCHC 1 cut(s) 95
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 3 cut(s) 266, 565, 631
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 2 cut(s) 182, 230
MmeI TCCRAC 2 cut(s) 156, 496
MnlI CCTC 8 cut(s) 82, 107, 110, 356, 364, 472, 475, 685
Mox20I TGGCCA 1 cut(s) 498
MscI TGGCCA 1 cut(s) 498
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 66
Msp20I TGGCCA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 3 cut(s) 28, 185, 192
MspR9I CCNGG 6 cut(s) 29, 125, 186, 192, 193, 243
MunI CAATTG 1 cut(s) 266
MvaI CCWGG 2 cut(s) 125, 243
MvnI CGCG 2 cut(s) 153, 155
MwoI GCNNNNNNNGC 4 cut(s) 150, 159, 315, 472
NciI CCSGG 4 cut(s) 29, 186, 192, 193
NdeI CATATG 1 cut(s) 700
NdeII GATC 6 cut(s) 178, 209, 220, 530, 582, 654
NlaIV GGNNCC 4 cut(s) 32, 189, 190, 273
NmeAIII GCCGAG 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 66
PaqCI CACCTGC 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 612
PfeI GAWTC 3 cut(s) 77, 287, 436
PflFI GACNNNGTC 1 cut(s) 186
PkrI GCNGC 3 cut(s) 48, 317, 448
PleI GAGTC 2 cut(s) 181, 230
PpsI GAGTC 2 cut(s) 181, 230
Ppu21I YACGTR 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 123, 241
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 2 cut(s) 123, 241
PspN4I GGNNCC 4 cut(s) 32, 189, 190, 273
PspPI GGNCC 3 cut(s) 31, 188, 227
PspPPI RGGWCCY 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 315
PsuI RGATCY 2 cut(s) 209, 582
PsyI GACNNNGTC 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 2 cut(s) 545, 676
RsaNI GTAC 2 cut(s) 544, 675
RseI CAYNNNNRTG 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 3 cut(s) 47, 316, 447
Sau3AI GATC 6 cut(s) 178, 209, 220, 530, 582, 654
Sau96I GGNCC 3 cut(s) 31, 188, 227
SchI GAGTC 2 cut(s) 182, 230
ScrFI CCNGG 6 cut(s) 29, 125, 186, 192, 193, 243
SduI GDGCHC 1 cut(s) 95
SfaNI GCATC 3 cut(s) 48, 463, 704
SinI GGWCC 2 cut(s) 31, 188
SmaI CCCGGG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 266, 565, 631
SsiI CCGC 4 cut(s) 10, 144, 151, 162
SspI AATATT 1 cut(s) 681
SspMI CTAG 2 cut(s) 86, 471
StyD4I CCNGG 6 cut(s) 27, 123, 184, 190, 191, 241
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 68, 505
TaiI ACGT 1 cut(s) 255
TaqI TCGA 2 cut(s) 171, 181
TaqII GACCGA 1 cut(s) 122
TasI AATT 3 cut(s) 266, 565, 631
TatI WGTACW 2 cut(s) 543, 674
TfiI GAWTC 3 cut(s) 77, 287, 436
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 1 cut(s) 629
TseI GCWGC 3 cut(s) 46, 315, 446
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 1 cut(s) 629
Tth111I GACNNNGTC 1 cut(s) 186
VpaK11BI GGWCC 2 cut(s) 31, 188
XagI CCTNNNNNAGG 1 cut(s) 354
XmaI CCCGGG 1 cut(s) 191
XspI CTAG 2 cut(s) 86, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.