Rw5G034010

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001406
Physical Location & Seq
Reverse (-)
58188458 .. 58189792
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034010.1

Sequence Viewer

Length: 1284 bp
ATGGCTGGTGTTGAGGATGTGACTTCGAAGCTTGCTGCGGCCCTTGCTTTTGGGGGAGATGACTTCCACAATGAGGATCCAGCGGTGGAGTTGGCAGATCTAACCTTAGTTGGTGGGGCGTCTGGTCTCTGTGGATCCGATGAAAGTGGCGCCGCACAGATCTGGCCTGTGGCTGACCATGGGTTGGTGATCCGTGAACGACCAAATAGGCGATTTCTCTTCCGCTTTGTTAATCCGACCGATAAGCAACGAGTTTTGGCTAAATCACTATGGTATTATCGTTCGGCGATGTTGGTTTTAGGGGAATATGATGGGGTGGGTGATATCATGGAGGTGACTCTGAATCGATTGCCGATTTGGATCCAGATCAGAGGATTACTGCCGGCCTATTCCACACCGAGTTGGGCAAGGAAGGTTGCTGGGTGCTTGGGTCGGGTTGTTCATGAGGATGAAAGGAGATTGAAGTGGCGTGAGGAAGTGAAGGTGTTGGTGGTGGTGGATCTCGTTCGTCCCCTTCCTCGCATTTTAGAGATTGATATTGAGTATTTGAATGTCAGGGGTTTGTGCTTGGTTTGTGGGTTCTTTGAGCATGGTGTGGGAGGTTGTGGTGGTCCTCTAAAGGAAGTGTGGAATACAGGTGTGGCTGTAACCTCGTCTAGAGGTGGACAGCGGAGTGTGGTTTTGCAGGGTGATTCATCGGTGGTGGTGGAGGCTGTTCCCCGTTCGAGTGGGATGGGAGTATCACCGGGTTTTGAAGCTTTACTGAGATCTAAGGGGGTGGGTGACCATCCTAGGATTGGGCCATTAATGTTGGAAGGGAAGATAGATTCTCGTCCGCTGGTGTTGGAGGAGAGTGTTGATGAGGTACAGGTTATTGTTAGGTCAATGCTTCATTGGAGGAGAGTGTTGTTGTTAGGTCAATGCTTGGGACAAAAGGGTGGTGATGGTGAAGTTACGTCTCCATTGAAGAAAGGGAGGTTGTGTTCTAAATCTGGTGTTGATCGAGGGCTTAGTGACTCATCTAGAGTTGTTGTGGGAGGTGTGCCATTGGCTCCTAGTAGTCCAGATTCCTTGGGTCATTGGAAGCATAGGATATCTGTTTCCACTTTCAATTTTGGGATGGAGGGCTCCCCTACCAAAGGCTTGCAGTTTGAGGTGGGGAAGTCTTCCTCTCCTGCTATTGGTAAGGTTGGGGATGGGAAGAAACATAAGCGAAAGATTATCTCAGCTGCAAAGCGTTGTGCACTGCTCTCTCGGCTTGAGGAGGAGGCCTCTGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

427

Amino Acids

46.06

Weight (kDa)

8.84

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 53 - 100 1.7e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 184
AciI CCGC 6 cut(s) 38, 83, 153, 223, 670, 836
AclWI GGATC 8 cut(s) 71, 84, 129, 142, 184, 355, 368, 507
AcyI GRCGYC 2 cut(s) 119, 150
AfaI GTAC 1 cut(s) 867
AfiI CCNNNNNNNGG 5 cut(s) 73, 184, 797, 1139, 1181
AgsI TTSAA 5 cut(s) 463, 550, 755, 967, 1111
AloI GAACNNNNNNTCC 2 cut(s) 967, 999
AluBI AGCT 3 cut(s) 31, 758, 1229
AluI AGCT 3 cut(s) 31, 758, 1229
Alw21I GWGCWC 1 cut(s) 1246
Alw26I GTCTC 2 cut(s) 131, 963
Alw44I GTGCAC 1 cut(s) 1242
AlwI GGATC 8 cut(s) 71, 84, 129, 142, 184, 355, 368, 507
AoxI GGCC 5 cut(s) 39, 164, 384, 800, 1269
ApaLI GTGCAC 1 cut(s) 1242
ApeKI GCWGC 2 cut(s) 35, 1229
AseI ATTAAT 1 cut(s) 806
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 40, 611, 800
AsuC2I CCSGG 1 cut(s) 747
AsuHPI GGTGA 8 cut(s) 199, 332, 346, 701, 735, 794, 953, 959
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 611
AvrII CCTAGG 1 cut(s) 791
BaeGI GKGCMC 1 cut(s) 1246
BamHI GGATCC 3 cut(s) 76, 134, 360
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 1130
BbsI GAAGAC 1 cut(s) 1158
Bbv12I GWGCWC 1 cut(s) 1246
BbvI GCAGC 2 cut(s) 22, 1216
BccI CCATC 6 cut(s) 305, 727, 795, 938, 1114, 1190
BcnI CCSGG 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 131, 963
BfaI CTAG 4 cut(s) 657, 792, 1023, 1056
BfoI RGCGCY 1 cut(s) 153
BglII AGATCT 3 cut(s) 97, 159, 767
BisI GCNGC 4 cut(s) 36, 39, 153, 1230
BlnI CCTAGG 1 cut(s) 791
BlsI GCNGC 4 cut(s) 37, 40, 154, 1231
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 611
BmgT120I GGNCC 3 cut(s) 40, 611, 800
BmiI GGNNCC 6 cut(s) 78, 136, 151, 362, 1053, 1129
BmrFI CCNGG 1 cut(s) 747
BpiI GAAGAC 1 cut(s) 1158
BplI GAGNNNNNCTC 1 cut(s) 1256
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1280
BpuMI CCSGG 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 346
BsaBI GATNNNNATC 2 cut(s) 359, 365
BsaHI GRCGYC 2 cut(s) 119, 150
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 178, 791, 1071
BsaXI ACNNNNNCTCC 2 cut(s) 967, 997
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 184, 797, 1139, 1181
Bse118I RCCGGY 1 cut(s) 382
Bse8I GATNNNNATC 2 cut(s) 359, 365
BseCI ATCGAT 1 cut(s) 346
BseDI CCNNGG 3 cut(s) 178, 791, 1071
BseGI GGATG 6 cut(s) 22, 454, 738, 787, 1125, 1201
BseJI GATNNNNATC 2 cut(s) 359, 365
BseLI CCNNNNNNNGG 5 cut(s) 73, 184, 797, 1139, 1181
BseMII CTCAG 2 cut(s) 755, 1239
BseRI GAGGAG 4 cut(s) 863, 913, 1277, 1280
BseSI GKGCMC 1 cut(s) 1246
BseXI GCAGC 2 cut(s) 22, 1216
BseYI CCCAGC 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 5 cut(s) 41, 166, 386, 802, 1271
BshNI GGYRCC 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 1246
BsiSI CCGG 2 cut(s) 383, 746
BslFI GGGAC 2 cut(s) 495, 942
BslI CCNNNNNNNGG 5 cut(s) 73, 184, 797, 1139, 1181
BsmAI GTCTC 2 cut(s) 131, 963
BsmBI CGTCTC 1 cut(s) 963
BsmFI GGGAC 2 cut(s) 495, 942
BsnI GGCC 5 cut(s) 41, 166, 386, 802, 1271
Bso31I GGTCTC 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 1130, 1246
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 6 cut(s) 38, 83, 153, 223, 670, 836
BspANI GGCC 5 cut(s) 41, 166, 386, 802, 1271
BspCNI CTCAG 2 cut(s) 756, 1238
BspDI ATCGAT 1 cut(s) 346
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 6 cut(s) 78, 136, 151, 362, 1053, 1129
BspPI GGATC 8 cut(s) 71, 84, 129, 142, 184, 355, 368, 507
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 178, 791, 1071
BssNI GRCGYC 2 cut(s) 119, 150
BssT1I CCWWGG 3 cut(s) 178, 791, 1071
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 119, 150
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 33, 384, 1145
BstDEI CTNAG 5 cut(s) 106, 764, 771, 1010, 1225
BstDSI CCRYGG 1 cut(s) 178
BstEII GGTNACC 1 cut(s) 782
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 6 cut(s) 22, 454, 738, 787, 1125, 1201
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 2 cut(s) 131, 963
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 2 cut(s) 44, 1255
BstPI GGTNACC 1 cut(s) 782
BstSCI CCNGG 1 cut(s) 745
BstSLI GKGCMC 1 cut(s) 1246
BstV1I GCAGC 2 cut(s) 22, 1216
BstV2I GAAGAC 1 cut(s) 1158
BstX2I RGATCY 7 cut(s) 76, 97, 134, 159, 360, 499, 767
BstYI RGATCY 7 cut(s) 76, 97, 134, 159, 360, 499, 767
Bsu15I ATCGAT 1 cut(s) 346
BsuRI GGCC 5 cut(s) 41, 166, 386, 802, 1271
BsuTUI ATCGAT 1 cut(s) 346
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 6 cut(s) 22, 454, 738, 787, 1125, 1201
BtsI GCAGTG 1 cut(s) 1244
BtsIMutI CAGTG 1 cut(s) 1244
Cac8I GCNNGC 3 cut(s) 33, 384, 1145
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 3 cut(s) 40, 611, 800
ClaI ATCGAT 1 cut(s) 346
CseI GACGC 1 cut(s) 108
Csp6I GTAC 1 cut(s) 866
CviAII CATG 4 cut(s) 179, 328, 443, 590
CviQI GTAC 1 cut(s) 866
DdeI CTNAG 5 cut(s) 106, 764, 771, 1010, 1225
DinI GGCGCC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 3 cut(s) 178, 791, 1071
Eco147I AGGCCT 1 cut(s) 1271
Eco24I GRGCYC 1 cut(s) 1130
Eco31I GGTCTC 1 cut(s) 131
Eco32I GATATC 2 cut(s) 325, 1095
Eco47I GGWCC 1 cut(s) 611
Eco91I GGTNACC 1 cut(s) 782
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoO65I GGTNACC 1 cut(s) 782
EcoRV GATATC 2 cut(s) 325, 1095
EcoT14I CCWWGG 3 cut(s) 178, 791, 1071
EcoT38I GRGCYC 1 cut(s) 1130
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 3 cut(s) 178, 791, 1071
Esp3I CGTCTC 1 cut(s) 963
FaeI CATG 4 cut(s) 182, 331, 446, 593
FaiI YATR 8 cut(s) 180, 271, 309, 329, 444, 591, 1089, 1209
FaqI GGGAC 2 cut(s) 495, 942
FatI CATG 4 cut(s) 178, 327, 442, 589
Fnu4HI GCNGC 4 cut(s) 36, 39, 153, 1230
FokI GGATG 6 cut(s) 29, 461, 745, 774, 1132, 1208
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 4 cut(s) 36, 39, 153, 1230
FspBI CTAG 4 cut(s) 657, 792, 1023, 1056
GlaI GCGC 1 cut(s) 151
GluI GCNGC 4 cut(s) 36, 39, 153, 1230
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 5 cut(s) 41, 166, 386, 802, 1271
HapII CCGG 2 cut(s) 383, 746
HgaI GACGC 1 cut(s) 108
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 2 cut(s) 119, 150
Hin1II CATG 4 cut(s) 182, 331, 446, 593
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HindIII AAGCTT 2 cut(s) 29, 756
HinfI GANTC 6 cut(s) 337, 343, 692, 827, 1016, 1067
HpaII CCGG 2 cut(s) 383, 746
HphI GGTGA 8 cut(s) 199, 332, 346, 701, 735, 794, 953, 959
Hpy166II GTNNAC 3 cut(s) 197, 665, 1244
Hpy188I TCNGA 4 cut(s) 139, 237, 342, 371
Hpy188III TCNNGA 5 cut(s) 364, 443, 657, 1023, 1064
Hpy8I GTNNAC 3 cut(s) 197, 665, 1244
HpyAV CCTTC 4 cut(s) 406, 475, 524, 809
HpyCH4IV ACGT 1 cut(s) 956
HpyCH4V TGCA 4 cut(s) 685, 1147, 1232, 1244
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 1255
HpyF3I CTNAG 5 cut(s) 106, 764, 771, 1010, 1225
HpySE526I ACGT 1 cut(s) 956
Hsp92I GRCGYC 2 cut(s) 119, 150
Hsp92II CATG 4 cut(s) 182, 331, 446, 593
HspAI GCGC 1 cut(s) 150
KasI GGCGCC 1 cut(s) 149
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
LmnI GCTCC 3 cut(s) 1057, 1133, 1282
Lsp1109I GCAGC 2 cut(s) 22, 1216
MaeI CTAG 4 cut(s) 657, 792, 1023, 1056
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 6 cut(s) 19, 334, 646, 782, 952, 1013
MboII GAAGA 5 cut(s) 211, 832, 979, 1158, 1213
MflI RGATCY 7 cut(s) 76, 97, 134, 159, 360, 499, 767
MhlI GDGCHC 2 cut(s) 1130, 1246
MluCI AATT 1 cut(s) 1111
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 331, 1010
MmeI TCCRAC 3 cut(s) 260, 792, 825
MroNI GCCGGC 1 cut(s) 382
MseI TTAA 2 cut(s) 231, 806
MslI CAYNNNNRTG 2 cut(s) 332, 447
MspA1I CMGCKG 4 cut(s) 83, 670, 838, 1229
MspI CCGG 2 cut(s) 383, 746
MspR9I CCNGG 1 cut(s) 747
MwoI GCNNNNNNNGC 2 cut(s) 44, 1255
NaeI GCCGGC 1 cut(s) 384
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 1 cut(s) 747
NcoI CCATGG 1 cut(s) 178
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 4 cut(s) 182, 331, 446, 593
NlaIV GGNNCC 6 cut(s) 78, 136, 151, 362, 1053, 1129
NmeAIII GCCGAG 1 cut(s) 1234
NmuCI GTSAC 4 cut(s) 19, 334, 782, 1013
NspV TTCGAA 1 cut(s) 26
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 1271
PdiI GCCGGC 1 cut(s) 384
PfeI GAWTC 4 cut(s) 343, 692, 827, 1067
PflMI CCANNNNNTGG 1 cut(s) 184
PkrI GCNGC 4 cut(s) 37, 40, 154, 1231
PleI GAGTC 2 cut(s) 331, 1010
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 2 cut(s) 331, 1010
PshBI ATTAAT 1 cut(s) 806
PspEI GGTNACC 1 cut(s) 782
PspFI CCCAGC 1 cut(s) 419
PspN4I GGNNCC 6 cut(s) 78, 136, 151, 362, 1053, 1129
PspPI GGNCC 3 cut(s) 40, 611, 800
PsuI RGATCY 7 cut(s) 76, 97, 134, 159, 360, 499, 767
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 1 cut(s) 867
RsaNI GTAC 1 cut(s) 866
RseI CAYNNNNRTG 2 cut(s) 332, 447
SaqAI TTAA 2 cut(s) 231, 806
SatI GCNGC 4 cut(s) 36, 39, 153, 1230
Sau96I GGNCC 3 cut(s) 40, 611, 800
SchI GAGTC 2 cut(s) 331, 1010
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 2 cut(s) 1130, 1246
SfoI GGCGCC 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 1 cut(s) 611
SmiMI CAYNNNNRTG 2 cut(s) 332, 447
SmlI CTYRAG 1 cut(s) 1259
SmoI CTYRAG 1 cut(s) 1259
Sse9I AATT 1 cut(s) 1111
SseBI AGGCCT 1 cut(s) 1271
SsiI CCGC 6 cut(s) 38, 83, 153, 223, 670, 836
SspDI GGCGCC 1 cut(s) 149
SspMI CTAG 4 cut(s) 657, 792, 1023, 1056
StuI AGGCCT 1 cut(s) 1271
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 3 cut(s) 178, 791, 1071
TaiI ACGT 1 cut(s) 959
TaqI TCGA 4 cut(s) 26, 346, 725, 1003
TaqII GACCGA 1 cut(s) 254
TasI AATT 1 cut(s) 1111
TauI GCSGC 2 cut(s) 41, 155
TfiI GAWTC 4 cut(s) 343, 692, 827, 1067
Tru1I TTAA 2 cut(s) 231, 806
Tru9I TTAA 2 cut(s) 231, 806
TscAI CASTG 1 cut(s) 1251
TseFI GTSAC 4 cut(s) 19, 334, 782, 1013
TseI GCWGC 2 cut(s) 35, 1229
Tsp45I GTSAC 4 cut(s) 19, 334, 782, 1013
TspDTI ATGAA 5 cut(s) 156, 431, 465, 684, 881
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 1 cut(s) 1251
Van91I CCANNNNNTGG 1 cut(s) 184
VneI GTGCAC 1 cut(s) 1242
VpaK11BI GGWCC 1 cut(s) 611
VspI ATTAAT 1 cut(s) 806
XagI CCTNNNNNAGG 1 cut(s) 1137
XbaI TCTAGA 2 cut(s) 656, 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XmaJI CCTAGG 1 cut(s) 791
XspI CTAG 4 cut(s) 657, 792, 1023, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.