pycom13g25150

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001766
Physical Location & Seq
Forward (+)
22070674 .. 22070958
285 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25150.1

Sequence Viewer

Length: 285 bp
ATGGATGGTGAAGAAGACCCGGATACGGTGTCGTTTCCACCGGACATGGTCCGCATGGAGACGCTCTTCCCCAAATCTCTCACTCGACTCAGAATAGTTGGCTTCCCGAATCTGAAGAAACTGAGCAGCAACGGCTTTCAATTCCTCACCTCCCTTCAATCTCTGTGGCTCTTCAATTGTCCAAAGCTAGCATCCATTCCAGAGGAGGGTCTGCCTCCTTCACTAGAGGAACTTCGCATCATTAGGTGTCCAGTGCTAAAAGAGAGATGCCAACCAGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

95

Amino Acids

10.68

Weight (kDa)

6.24

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 25, 206
AgsI TTSAA 3 cut(s) 140, 158, 175
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 53
ApeKI GCWGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 49
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 20, 139
AsuNHI GCTAGC 1 cut(s) 187
AvaII GGWCC 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 138
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 276
BciVI GTATCC 1 cut(s) 16
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 188, 224
BfuI GTATCC 1 cut(s) 16
BisI GCNGC 1 cut(s) 127
BlsI GCNGC 1 cut(s) 128
Bme1390I CCNGG 2 cut(s) 20, 276
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmrFI CCNGG 2 cut(s) 20, 276
BmsI GCATC 3 cut(s) 200, 246, 257
BmtI GCTAGC 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 206
Bse1I ACTGG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 10, 191
BseLI CCNNNNNNNGG 2 cut(s) 25, 206
BseMII CTCAG 2 cut(s) 103, 113
BseNI ACTGG 1 cut(s) 251
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 138
BsiSI CCGG 2 cut(s) 20, 41
BslI CCNNNNNNNGG 2 cut(s) 25, 206
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BspACI CCGC 1 cut(s) 52
BspCNI CTCAG 2 cut(s) 102, 114
BspOI GCTAGC 1 cut(s) 191
BspQI GCTCTTC 2 cut(s) 71, 176
BsrI ACTGG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 2 cut(s) 71, 176
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 89, 122
BstF5I GGATG 2 cut(s) 10, 191
BstMAI GTCTC 1 cut(s) 53
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 18, 274
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 21
BsuI GTATCC 1 cut(s) 16
BtsCI GGATG 2 cut(s) 10, 191
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 1 cut(s) 70
CspCI CAANNNNNGTGG 2 cut(s) 146, 181
CviAII CATG 2 cut(s) 46, 55
CviJI RGCY 4 cut(s) 102, 135, 169, 187
CviKI_1 RGCY 4 cut(s) 102, 135, 169, 187
DdeI CTNAG 2 cut(s) 89, 122
Eam1104I CTCTTC 2 cut(s) 71, 176
EarI CTCTTC 2 cut(s) 71, 176
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 2 cut(s) 49, 58
FaiI YATR 2 cut(s) 47, 56
FatI CATG 2 cut(s) 45, 54
Fnu4HI GCNGC 1 cut(s) 127
FokI GGATG 2 cut(s) 17, 178
Fsp4HI GCNGC 1 cut(s) 127
FspBI CTAG 2 cut(s) 188, 224
GluI GCNGC 1 cut(s) 127
HapII CCGG 2 cut(s) 20, 41
HgaI GACGC 1 cut(s) 70
Hin1II CATG 2 cut(s) 49, 58
HinfI GANTC 2 cut(s) 87, 109
HpaII CCGG 2 cut(s) 20, 41
HphI GGTGA 2 cut(s) 20, 139
Hpy188I TCNGA 2 cut(s) 92, 114
Hpy188III TCNNGA 2 cut(s) 106, 200
HpyAV CCTTC 2 cut(s) 164, 228
HpyCH4III ACNGT 1 cut(s) 28
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 2 cut(s) 89, 122
Hsp92II CATG 2 cut(s) 49, 58
LguI GCTCTTC 2 cut(s) 71, 176
LpnPI CCDG 5 cut(s) 33, 54, 213, 261, 264
Lsp1109I GCAGC 1 cut(s) 138
LweI GCATC 3 cut(s) 200, 246, 257
MaeI CTAG 2 cut(s) 188, 224
MboII GAAGA 5 cut(s) 23, 26, 58, 127, 163
MfeI CAATTG 1 cut(s) 175
MluCI AATT 2 cut(s) 140, 175
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 6 cut(s) 155, 160, 196, 199, 220, 225
MspI CCGG 2 cut(s) 20, 41
MspR9I CCNGG 2 cut(s) 20, 276
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 132
NciI CCSGG 1 cut(s) 20
NheI GCTAGC 1 cut(s) 187
NlaIII CATG 2 cut(s) 49, 58
PciSI GCTCTTC 2 cut(s) 71, 176
PfeI GAWTC 1 cut(s) 109
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 1 cut(s) 128
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspPI GGNCC 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 71, 176
SatI GCNGC 1 cut(s) 127
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 20, 276
SetI ASST 3 cut(s) 152, 189, 248
SfaNI GCATC 3 cut(s) 200, 246, 257
SinI GGWCC 1 cut(s) 49
Sse9I AATT 2 cut(s) 140, 175
SsiI CCGC 1 cut(s) 52
SspMI CTAG 2 cut(s) 188, 224
StyD4I CCNGG 2 cut(s) 18, 274
TaaI ACNGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 85
TasI AATT 2 cut(s) 140, 175
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 1 cut(s) 258
TseI GCWGC 1 cut(s) 126
TspRI CASTG 1 cut(s) 258
Tth111I GACNNNGTC 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 49
XspI CTAG 2 cut(s) 188, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.