Rh1BG042200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001766
Physical Location & Seq
Forward (+)
5592736 .. 5594022
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG042200.1

Sequence Viewer

Length: 1287 bp
ATGGACAGATTCAAGAGAGACTTAACAAAGCTTAAAAATCTGAGAATTACTAGTTGTGAGGAGCTGACATCTTTATGGAAAAATGAGGATAGATTGCTGCAACGTCCGATTTATAAAGGAAACAGCTCTCACTCTTACTTTGTTCAAGAGCTGAAGTCACTTCAATGTCTTCACATAAATGATTGCTCAAATCTAGTTTCTTTTTCAAAGGTTAGCCTGCCACCTTCTCTTAAAGAAATAAAGATTGAGCGGTGCAGTTCTCTTATAAGATACCAGATACCCCCAAACGTAAGAAGTCTATACATCGATTCCTGCATAAACTTGGAATCATTAGTGGGGGAGGACAAGGAAGGTGAGGATCCTTCATCTTCCTTTTCTCCTTGTTTGATACAAGAGGAGGCATCCTGCCTCGAGTACTTGAGCATAGTGAATTGTATGAGATTTACAACCTTATCATCCACAGGCCAGCTAGCCAGGGGACTTAAACATATTATCATAAATCATTGTCACCAGCTAGAATCAATAACTGATAGCTTCCATGTTAACACCTTCCTTGAAGAAATTGTGATTAGGAGTTGTGGAAATCTTAAATCGTTACCAGAGGGCTTATGTGACCTCACCAATCTTCAAGTCTTTAAGATTTGGGACTGTGAAAGTCTTGTTTCTTTCCCAAGAGGAGGGTTGCCAACAAGAACCTCCAGCTTGAAAGAGATCGAGGTTATGAATTGTGATAACTTAGATGCCCTTCCTAAAGACATGCAGAACCTCCACTGTCTTCAGAGTTTGAGGATTTCATACTGTGAAGGTTTCGCTCCATTTCTAGACAAGGGTTTGCCACCCAACCTAACATTCCTTGAGATTCGAAATCTCAACAGTTGTAGGCCACTCCTGGAAGCTCAAGGATTGCACAGACTCACTTCTCTTAGAGCGTTGGATATCAGGGGAAATGATTCACAACTAGTGTCATTTCCACCTGACAAGGAGATGGTGCTCCCCAAATCTCTCATTCTACTTCATATTGAAGGGTTCCCGAATCTGAGGCACCTATCCAGCAAGGGCTTTCAGTTCCTACCCTCTCTTGAATCTCTTCAAGTCATCAGCTGTCCAAAGCTAGCATCCATTCCTGAGGAGGGCCTCCCTCTTTCACTTACACAACTTCATATCTTTTTTTGTCCACTGCTAGTAAAGAACTGCAGACCTGGAAAAGGACAATACTGGCACTCCATAGCTCGCATCCCTTTCATATGGATTAAGGGTGATAAGGGAGCAGACATATTTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

428

Amino Acids

48.5

Weight (kDa)

7.13

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 195 - 364 7.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 114, 266
AccB1I GGYRCC 1 cut(s) 1041
AccBSI CCGCTC 1 cut(s) 250
AciI CCGC 1 cut(s) 250
AclWI GGATC 2 cut(s) 353, 366
AcuI CTGAAG 2 cut(s) 173, 761
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 3 cut(s) 677, 1130, 1205
AhlI ACTAGT 2 cut(s) 50, 958
AjnI CCWGG 3 cut(s) 473, 888, 1198
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 2 cut(s) 353, 366
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 3 cut(s) 463, 881, 1132
ApeKI GCWGC 1 cut(s) 97
Asp700I GAANNNNTTC 2 cut(s) 949, 1086
AspS9I GGNCC 1 cut(s) 1132
AsuHPI GGTGA 4 cut(s) 365, 500, 610, 1268
AsuII TTCGAA 1 cut(s) 862
AsuNHI GCTAGC 2 cut(s) 469, 1111
AvaI CYCGRG 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 1125
BamHI GGATCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 1041
BbsI GAAGAC 2 cut(s) 161, 767
Bbv12I GWGCWC 1 cut(s) 993
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 979
BciT130I CCWGG 3 cut(s) 475, 890, 1200
BcoDI GTCTC 1 cut(s) 12
BcuI ACTAGT 2 cut(s) 50, 958
BfaI CTAG 8 cut(s) 51, 194, 470, 515, 821, 959, 1112, 1181
BfmI CTRYAG 1 cut(s) 1192
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 475, 890, 1200
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 1132
BmiI GGNNCC 3 cut(s) 360, 1028, 1043
BmrFI CCNGG 3 cut(s) 475, 890, 1200
BmsI GCATC 4 cut(s) 410, 730, 1124, 1242
BmtI GCTAGC 2 cut(s) 473, 1115
BpiI GAAGAC 2 cut(s) 161, 767
BpmI CTGGAG 1 cut(s) 682
Bpu14I TTCGAA 1 cut(s) 862
BpuEI CTTGAG 3 cut(s) 439, 875, 882
Bsa29I ATCGAT 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 474
BsaXI ACNNNNNCTCC 2 cut(s) 1205, 1235
Bsc4I CCNNNNNNNGG 3 cut(s) 677, 1130, 1205
Bse1I ACTGG 1 cut(s) 1220
Bse21I CCTNAGG 1 cut(s) 1125
BseBI CCWGG 3 cut(s) 475, 890, 1200
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 4 cut(s) 401, 455, 1115, 1233
BseLI CCNNNNNNNGG 3 cut(s) 677, 1130, 1205
BseMII CTCAG 3 cut(s) 32, 1028, 1116
BseNI ACTGG 1 cut(s) 1220
BseRI GAGGAG 4 cut(s) 74, 410, 690, 1142
BseXI GCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 274
BshFI GGCC 3 cut(s) 465, 883, 1134
BshNI GGYRCC 1 cut(s) 1041
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 1 cut(s) 410
BslFI GGGAC 2 cut(s) 492, 659
BslI CCNNNNNNNGG 3 cut(s) 677, 1130, 1205
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 492, 659
BsnI GGCC 3 cut(s) 465, 883, 1134
BsoBI CYCGRG 1 cut(s) 410
Bsp119I TTCGAA 1 cut(s) 862
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 2 cut(s) 358, 711
BspACI CCGC 1 cut(s) 250
BspANI GGCC 3 cut(s) 465, 883, 1134
BspCNI CTCAG 3 cut(s) 33, 1029, 1117
BspDI ATCGAT 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 360, 1028, 1043
BspMAI CTGCAG 1 cut(s) 1196
BspOI GCTAGC 2 cut(s) 473, 1115
BspPI GGATC 2 cut(s) 353, 366
BspT104I TTCGAA 1 cut(s) 862
BspT107I GGYRCC 1 cut(s) 1041
BsrBI CCGCTC 1 cut(s) 250
BsrI ACTGG 1 cut(s) 1220
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 2 cut(s) 358, 711
Bst2UI CCWGG 3 cut(s) 475, 890, 1200
Bst4CI ACNGT 4 cut(s) 650, 773, 800, 875
Bst6I CTCTTC 1 cut(s) 1092
BstBI TTCGAA 1 cut(s) 862
BstC8I GCNNGC 5 cut(s) 218, 467, 471, 1113, 1231
BstDEI CTNAG 5 cut(s) 41, 736, 923, 1037, 1125
BstENI CCTNNNNNAGG 1 cut(s) 1203
BstF5I GGATG 4 cut(s) 401, 455, 1115, 1233
BstKTI GATC 2 cut(s) 361, 714
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 358, 711
BstNI CCWGG 3 cut(s) 475, 890, 1200
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 3 cut(s) 473, 888, 1198
BstSFI CTRYAG 1 cut(s) 1192
BstV1I GCAGC 1 cut(s) 84
BstV2I GAAGAC 2 cut(s) 161, 767
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
Bsu15I ATCGAT 1 cut(s) 306
Bsu36I CCTNAGG 1 cut(s) 1125
BsuRI GGCC 3 cut(s) 465, 883, 1134
BsuTUI ATCGAT 1 cut(s) 306
BtsCI GGATG 4 cut(s) 401, 455, 1115, 1233
BtsI GCAGTG 1 cut(s) 1175
BtsIMutI CAGTG 2 cut(s) 769, 1175
Cac8I GCNNGC 5 cut(s) 218, 467, 471, 1113, 1231
Cfr13I GGNCC 1 cut(s) 1132
ClaI ATCGAT 1 cut(s) 306
Csp6I GTAC 1 cut(s) 415
CviAII CATG 2 cut(s) 539, 757
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 5 cut(s) 41, 736, 923, 1037, 1125
DpnI GATC 2 cut(s) 360, 713
DpnII GATC 2 cut(s) 358, 711
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
Eco32I GATATC 1 cut(s) 937
Eco57I CTGAAG 2 cut(s) 173, 761
Eco81I CCTNAGG 1 cut(s) 1125
Eco88I CYCGRG 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 1203
EcoO109I RGGNCCY 1 cut(s) 1132
EcoRII CCWGG 3 cut(s) 473, 888, 1198
EcoRV GATATC 1 cut(s) 937
FaeI CATG 2 cut(s) 542, 760
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 492, 659
FatI CATG 2 cut(s) 538, 756
FauNDI CATATG 1 cut(s) 1244
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 4 cut(s) 388, 442, 1102, 1220
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 8 cut(s) 51, 194, 470, 515, 821, 959, 1112, 1181
GluI GCNGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 682
HaeIII GGCC 3 cut(s) 465, 883, 1134
Hin1II CATG 2 cut(s) 542, 760
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 9 cut(s) 9, 308, 326, 518, 859, 912, 950, 1033, 1082
HpaI GTTAAC 1 cut(s) 544
HphI GGTGA 4 cut(s) 365, 500, 610, 1268
Hpy166II GTNNAC 2 cut(s) 544, 1175
Hpy188I TCNGA 4 cut(s) 42, 108, 780, 1038
Hpy188III TCNNGA 6 cut(s) 13, 146, 821, 1030, 1079, 1124
Hpy8I GTNNAC 2 cut(s) 544, 1175
HpyAV CCTTC 7 cut(s) 234, 344, 372, 559, 755, 797, 1016
HpyCH4III ACNGT 4 cut(s) 650, 773, 800, 875
HpyCH4IV ACGT 2 cut(s) 103, 288
HpyCH4V TGCA 6 cut(s) 100, 255, 315, 760, 907, 1194
HpyF3I CTNAG 5 cut(s) 41, 736, 923, 1037, 1125
HpySE526I ACGT 2 cut(s) 103, 288
Hsp92II CATG 2 cut(s) 542, 760
KspAI GTTAAC 1 cut(s) 544
Kzo9I GATC 2 cut(s) 358, 711
LmnI GCTCC 4 cut(s) 61, 817, 996, 1265
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 4 cut(s) 410, 730, 1124, 1242
MaeI CTAG 8 cut(s) 51, 194, 470, 515, 821, 959, 1112, 1181
MaeII ACGT 2 cut(s) 103, 288
MaeIII GTNAC 4 cut(s) 156, 506, 594, 611
MalI GATC 2 cut(s) 360, 713
MbiI CCGCTC 1 cut(s) 250
MboI GATC 2 cut(s) 358, 711
MboII GAAGA 6 cut(s) 161, 360, 569, 617, 767, 1079
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 993
MluCI AATT 4 cut(s) 45, 430, 561, 724
MlyI GAGTC 1 cut(s) 906
MmeI TCCRAC 1 cut(s) 912
MroXI GAANNNNTTC 2 cut(s) 949, 1086
MseI TTAA 8 cut(s) 23, 33, 231, 483, 543, 588, 636, 1251
MslI CAYNNNNRTG 3 cut(s) 73, 163, 177
MspA1I CMGCKG 1 cut(s) 1101
MspR9I CCNGG 3 cut(s) 475, 890, 1200
MvaI CCWGG 3 cut(s) 475, 890, 1200
NdeI CATATG 1 cut(s) 1244
NdeII GATC 2 cut(s) 358, 711
NheI GCTAGC 2 cut(s) 469, 1111
NlaIII CATG 2 cut(s) 542, 760
NlaIV GGNNCC 3 cut(s) 360, 1028, 1043
NmuCI GTSAC 3 cut(s) 156, 506, 611
NspI RCATGY 1 cut(s) 760
NspV TTCGAA 1 cut(s) 862
PaeR7I CTCGAG 1 cut(s) 410
PdmI GAANNNNTTC 2 cut(s) 949, 1086
PfeI GAWTC 8 cut(s) 9, 308, 326, 518, 859, 950, 1033, 1082
PfoI TCCNGGA 1 cut(s) 888
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 906
PpsI GAGTC 1 cut(s) 906
PsiI TTATAA 2 cut(s) 114, 266
Psp6I CCWGG 3 cut(s) 473, 888, 1198
PspGI CCWGG 3 cut(s) 473, 888, 1198
PspN4I GGNNCC 3 cut(s) 360, 1028, 1043
PspPI GGNCC 1 cut(s) 1132
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 1196
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 1101
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 3 cut(s) 73, 163, 177
SaqAI TTAA 8 cut(s) 23, 33, 231, 483, 543, 588, 636, 1251
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 358, 711
Sau96I GGNCC 1 cut(s) 1132
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 1 cut(s) 906
ScrFI CCNGG 3 cut(s) 475, 890, 1200
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 4 cut(s) 410, 730, 1124, 1242
SfcI CTRYAG 1 cut(s) 1192
Sfr274I CTCGAG 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 3 cut(s) 73, 163, 177
SmlI CTYRAG 4 cut(s) 410, 418, 854, 897
SmoI CTYRAG 4 cut(s) 410, 418, 854, 897
SpeI ACTAGT 2 cut(s) 50, 958
Sse9I AATT 4 cut(s) 45, 430, 561, 724
SsiI CCGC 1 cut(s) 250
SspMI CTAG 8 cut(s) 51, 194, 470, 515, 821, 959, 1112, 1181
StyD4I CCNGG 3 cut(s) 473, 888, 1198
TaaI ACNGT 4 cut(s) 650, 773, 800, 875
TaiI ACGT 2 cut(s) 106, 291
TaqI TCGA 4 cut(s) 306, 411, 714, 862
TasI AATT 4 cut(s) 45, 430, 561, 724
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 8 cut(s) 9, 308, 326, 518, 859, 950, 1033, 1082
Tru1I TTAA 8 cut(s) 23, 33, 231, 483, 543, 588, 636, 1251
Tru9I TTAA 8 cut(s) 23, 33, 231, 483, 543, 588, 636, 1251
TscAI CASTG 2 cut(s) 776, 1182
TseFI GTSAC 3 cut(s) 156, 506, 611
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 3 cut(s) 156, 506, 611
TspDTI ATGAA 6 cut(s) 354, 737, 783, 1004, 1148, 1231
TspRI CASTG 2 cut(s) 776, 1182
XagI CCTNNNNNAGG 1 cut(s) 1203
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 1 cut(s) 760
XhoI CTCGAG 1 cut(s) 410
XmnI GAANNNNTTC 2 cut(s) 949, 1086
XspI CTAG 8 cut(s) 51, 194, 470, 515, 821, 959, 1112, 1181
ZrmI AGTACT 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.