RchiOBHm_Chr4g0408891

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001766
Physical Location & Seq
Reverse (-)
31833357 .. 31836919
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37996

Sequence Viewer

Length: 879 bp
ATGCAAGAGGATACATCTTGTCTTGAGTACTTGGAGGTATGGGAGTGTCCATCTCTTACATCCTTATCATCAAGAGGCCAACTACCCAGAGCGCTTAAACACCTTCACATAAGGGATTGTGAACGACTGGAGTCAATAACAGATATGTTCCACAGTGAAACTTGTCTTGAACATATTGTGATACAGAGTTGTGCAAATCTTAAATCCTTACCAGAAGGCCTATGCGACCTCATGCATCTTCAACAGTTAATTGTTATGGATTGTGGAAGTCTGGTTTCCTTCCCGAGAGGAGGGTTGCCAACAACAGTCTCCAACCTGACAAGCATCTATATCTACAATTGTGATAGCTTGGAAGCTTTCCCCAGAGGAATGGACAAGTACATCTCTCTTCAGACATTGTGGATCGGTTACTGTAAAGGTTTGTCATCCATTTTAGAAGAGGGTTTTTCCCCAAACTTAGTTGAGCTTTACATTGTGAACCCTTTACGTTGCACGCCACTCTGTGAATGGGGGTTGCAGTTGCACAGACTCAATTCTCTGAGAGAGTTGTGGATCCAGGGTGTAGATCCAAATCTGGTGTTATTTCCACCAGAGGAGATGGAGATGCTGCTCCCTAAATCTCTCATTAAAATCAGAATTGGAGACTTCCCAAAGCTAAGGCGCCTATCCAGCAAGGCCCTTCAGTCGCTCACCTCTCTTGAATCTCTTAGAATTGAAGATTGTCCAAAGCTAGCATCCATTCCAGAGGAGAAGCTGTCTCTTTCACTTACACAACTGCATATCTCCAATTGTCCTCTGCTAAAGAAGAGATACAGACCAGGGAAAGCACCACACTGGCCCAAAATAGGCCACATTCCTTACATACAGATTGGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

292

Amino Acids

33.21

Weight (kDa)

6.16

Isoelectric Point (pI)

56.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 5 - 139 1.8e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 660
AclWI GGATC 4 cut(s) 410, 547, 560, 560
AcuI CTGAAG 2 cut(s) 374, 665
AcyI GRCGYC 1 cut(s) 661
AdeI CACNNNGTG 1 cut(s) 503
AfaI GTAC 2 cut(s) 29, 380
AfeI AGCGCT 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 290, 845
AgsI TTSAA 4 cut(s) 170, 242, 701, 716
AjnI CCWGG 2 cut(s) 555, 817
AluBI AGCT 6 cut(s) 348, 356, 466, 655, 730, 754
AluI AGCT 6 cut(s) 348, 356, 466, 655, 730, 754
Alw26I GTCTC 3 cut(s) 313, 636, 762
AlwI GGATC 4 cut(s) 410, 547, 560, 560
Ama87I CYCGRG 1 cut(s) 283
Aor51HI AGCGCT 1 cut(s) 93
AoxI GGCC 5 cut(s) 76, 217, 675, 836, 847
ApeKI GCWGC 1 cut(s) 607
AspLEI GCGC 2 cut(s) 94, 663
AspS9I GGNCC 2 cut(s) 676, 837
AsuHPI GGTGA 1 cut(s) 682
AsuNHI GCTAGC 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 283
BamHI GGATCC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 660
BbvI GCAGC 1 cut(s) 594
BccI CCATC 2 cut(s) 58, 592
BciT130I CCWGG 2 cut(s) 557, 819
BciVI GTATCC 1 cut(s) 4
BcoDI GTCTC 3 cut(s) 313, 636, 762
BfaI CTAG 1 cut(s) 731
BfoI RGCGCY 2 cut(s) 95, 664
BfuI GTATCC 1 cut(s) 4
BisI GCNGC 1 cut(s) 608
BlsI GCNGC 1 cut(s) 609
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 2 cut(s) 557, 819
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 676, 837
BmiI GGNNCC 2 cut(s) 554, 662
BmrFI CCNGG 2 cut(s) 557, 819
BmsI GCATC 4 cut(s) 244, 333, 594, 743
BmtI GCTAGC 1 cut(s) 734
BoxI GACNNNNGTC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 656
BpuEI CTTGAG 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 661
BsaJI CCNNGG 2 cut(s) 556, 818
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 845
Bse1I ACTGG 2 cut(s) 132, 839
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 2 cut(s) 557, 819
BseDI CCNNGG 2 cut(s) 556, 818
BseGI GGATG 3 cut(s) 59, 425, 734
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 290, 845
BseMII CTCAG 1 cut(s) 530
BseNI ACTGG 2 cut(s) 132, 839
BseRI GAGGAG 3 cut(s) 303, 608, 761
BseXI GCAGC 1 cut(s) 594
BshFI GGCC 5 cut(s) 78, 219, 677, 838, 849
BshNI GGYRCC 1 cut(s) 660
BsiHKCI CYCGRG 1 cut(s) 283
BslI CCNNNNNNNGG 2 cut(s) 290, 845
BsmAI GTCTC 3 cut(s) 313, 636, 762
BsnI GGCC 5 cut(s) 78, 219, 677, 838, 849
BsoBI CYCGRG 1 cut(s) 283
Bsp143I GATC 3 cut(s) 402, 552, 565
BspANI GGCC 5 cut(s) 78, 219, 677, 838, 849
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 2 cut(s) 554, 662
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 4 cut(s) 410, 547, 560, 560
BspT107I GGYRCC 1 cut(s) 660
BsrI ACTGG 2 cut(s) 132, 839
BssECI CCNNGG 2 cut(s) 556, 818
BssMI GATC 3 cut(s) 402, 552, 565
BssNI GRCGYC 1 cut(s) 661
Bst2UI CCWGG 2 cut(s) 557, 819
Bst4CI ACNGT 4 cut(s) 155, 246, 307, 413
Bst6I CTCTTC 3 cut(s) 393, 432, 800
BstACI GRCGYC 1 cut(s) 661
BstC8I GCNNGC 2 cut(s) 494, 732
BstDEI CTNAG 4 cut(s) 457, 539, 656, 707
BstF5I GGATG 3 cut(s) 59, 425, 734
BstH2I RGCGCY 2 cut(s) 95, 664
BstHHI GCGC 2 cut(s) 94, 663
BstKTI GATC 3 cut(s) 405, 555, 568
BstMAI GTCTC 3 cut(s) 313, 636, 762
BstMBI GATC 3 cut(s) 402, 552, 565
BstMWI GCNNNNNNNGC 1 cut(s) 669
BstNI CCWGG 2 cut(s) 557, 819
BstPAI GACNNNNGTC 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 555, 817
BstV1I GCAGC 1 cut(s) 594
BstX2I RGATCY 2 cut(s) 552, 565
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 552, 565
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 5 cut(s) 78, 219, 677, 838, 849
BtsCI GGATG 3 cut(s) 59, 425, 734
BtsIMutI CAGTG 2 cut(s) 160, 832
Cac8I GCNNGC 2 cut(s) 494, 732
CfoI GCGC 2 cut(s) 94, 663
Cfr13I GGNCC 2 cut(s) 676, 837
Csp6I GTAC 2 cut(s) 28, 379
CviAII CATG 1 cut(s) 232
CviQI GTAC 2 cut(s) 28, 379
DdeI CTNAG 4 cut(s) 457, 539, 656, 707
DinI GGCGCC 1 cut(s) 662
DpnI GATC 3 cut(s) 404, 554, 567
DpnII GATC 3 cut(s) 402, 552, 565
DraIII CACNNNGTG 1 cut(s) 503
Eam1104I CTCTTC 3 cut(s) 393, 432, 800
EarI CTCTTC 3 cut(s) 393, 432, 800
Eco147I AGGCCT 1 cut(s) 219
Eco47III AGCGCT 1 cut(s) 93
Eco57I CTGAAG 2 cut(s) 374, 665
Eco88I CYCGRG 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 555, 817
EcoT22I ATGCAT 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 662
EheI GGCGCC 1 cut(s) 662
FaeI CATG 1 cut(s) 235
FatI CATG 1 cut(s) 231
Fnu4HI GCNGC 1 cut(s) 608
FokI GGATG 3 cut(s) 46, 412, 721
Fsp4HI GCNGC 1 cut(s) 608
FspBI CTAG 1 cut(s) 731
GlaI GCGC 2 cut(s) 93, 662
GluI GCNGC 1 cut(s) 608
GsuI CTGGAG 1 cut(s) 149
HaeII RGCGCY 2 cut(s) 95, 664
HaeIII GGCC 5 cut(s) 78, 219, 677, 838, 849
HhaI GCGC 2 cut(s) 94, 663
Hin1I GRCGYC 1 cut(s) 661
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 2 cut(s) 92, 661
HinP1I GCGC 2 cut(s) 92, 661
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 3 cut(s) 131, 528, 701
HphI GGTGA 1 cut(s) 682
Hpy166II GTNNAC 2 cut(s) 122, 478
Hpy188I TCNGA 3 cut(s) 393, 540, 635
Hpy188III TCNNGA 6 cut(s) 23, 72, 167, 283, 698, 743
Hpy8I GTNNAC 2 cut(s) 122, 478
HpyAV CCTTC 4 cut(s) 113, 209, 289, 689
HpyCH4III ACNGT 4 cut(s) 155, 246, 307, 413
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 7 cut(s) 4, 194, 235, 492, 517, 523, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 669
HpyF3I CTNAG 4 cut(s) 457, 539, 656, 707
HpySE526I ACGT 1 cut(s) 487
Hsp92I GRCGYC 1 cut(s) 661
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 2 cut(s) 92, 661
KasI GGCGCC 1 cut(s) 660
Kzo9I GATC 3 cut(s) 402, 552, 565
LmnI GCTCC 1 cut(s) 615
Lsp1109I GCAGC 1 cut(s) 594
LweI GCATC 4 cut(s) 244, 333, 594, 743
MaeI CTAG 1 cut(s) 731
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 1 cut(s) 407
MalI GATC 3 cut(s) 404, 554, 567
MboI GATC 3 cut(s) 402, 552, 565
MboII GAAGA 5 cut(s) 230, 380, 449, 728, 817
MfeI CAATTG 2 cut(s) 337, 787
MflI RGATCY 2 cut(s) 552, 565
MluCI AATT 6 cut(s) 249, 337, 532, 636, 711, 787
Mly113I GGCGCC 1 cut(s) 661
MlyI GAGTC 2 cut(s) 140, 522
MmeI TCCRAC 1 cut(s) 336
Mph1103I ATGCAT 1 cut(s) 237
MseI TTAA 4 cut(s) 96, 201, 248, 627
MspR9I CCNGG 2 cut(s) 557, 819
MunI CAATTG 2 cut(s) 337, 787
MvaI CCWGG 2 cut(s) 557, 819
MwoI GCNNNNNNNGC 1 cut(s) 669
NarI GGCGCC 1 cut(s) 661
NdeII GATC 3 cut(s) 402, 552, 565
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 2 cut(s) 554, 662
NsiI ATGCAT 1 cut(s) 237
PceI AGGCCT 1 cut(s) 219
PfeI GAWTC 1 cut(s) 701
PkrI GCNGC 1 cut(s) 609
PleI GAGTC 2 cut(s) 139, 522
PluTI GGCGCC 1 cut(s) 664
PpsI GAGTC 2 cut(s) 139, 522
PshAI GACNNNNGTC 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 555, 817
PspGI CCWGG 2 cut(s) 555, 817
PspN4I GGNNCC 2 cut(s) 554, 662
PspPI GGNCC 2 cut(s) 676, 837
PsuI RGATCY 2 cut(s) 552, 565
RsaI GTAC 2 cut(s) 29, 380
RsaNI GTAC 2 cut(s) 28, 379
SaqAI TTAA 4 cut(s) 96, 201, 248, 627
SatI GCNGC 1 cut(s) 608
Sau3AI GATC 3 cut(s) 402, 552, 565
Sau96I GGNCC 2 cut(s) 676, 837
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 2 cut(s) 140, 522
ScrFI CCNGG 2 cut(s) 557, 819
SfaNI GCATC 4 cut(s) 244, 333, 594, 743
SfoI GGCGCC 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 6 cut(s) 249, 337, 532, 636, 711, 787
SseBI AGGCCT 1 cut(s) 219
SspDI GGCGCC 1 cut(s) 660
SspMI CTAG 1 cut(s) 731
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 555, 817
TaaI ACNGT 4 cut(s) 155, 246, 307, 413
TaiI ACGT 1 cut(s) 490
TasI AATT 6 cut(s) 249, 337, 532, 636, 711, 787
TatI WGTACW 2 cut(s) 27, 378
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 4 cut(s) 96, 201, 248, 627
Tru9I TTAA 4 cut(s) 96, 201, 248, 627
TscAI CASTG 2 cut(s) 160, 839
TseI GCWGC 1 cut(s) 607
TspRI CASTG 2 cut(s) 160, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XspI CTAG 1 cut(s) 731
ZrmI AGTACT 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.