RchiOBHm_Chr5g0080601

Aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001407
Physical Location & Seq
Reverse (-)
86591013 .. 86596067
5055 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35491

Sequence Viewer

Length: 2940 bp
ATGATGATTGAAGGTTTCTATGGTGGTTTGTTACCACATGAGAGGAGGAGGGTTGATGTTGCCGCACAAGGCTCACTTCATAGTCATGGTCAAACCGAGGCATGGGATATACTTGAGCACTTGGGTCGACAATCGAGGCAATGGGATGATCATGATTCTAGGAGGGAAAGAGTGAGGAAAGATCCATATTATGATACCAAGAGCACTCATGAGACAAGGGTTGCCCAAGAGAGCTCTAGTGCCGATTATAGGAAGCTTGAGAGAAAGCTTGATTTACTTCTTCAAGCTCAAGAGCGTGGTTCATCTACACATCATGTCAAGGCCGCTTCTATGTCTTCATCTCTATGTCTTCTATGTGATTCACCTATACATGCTACTAGTGAATGTCATCTTGCTCCTAATTATCCGGATTTTGTTCAAGAGCAAGTAAAAGCCGTGAATTCTTATGGTGGGCACCCTAGAAATGATCCTTACTCACCTACATACAATCCGGGTTGGAGGAATCACCCTAATTTTTCTTGGAGAGACCAAGGAGGTTCATCTAGTGGGTTTCAAGGTGGAAAGCAAAGCTTTAGAGGTGGCCAAGGTCAAGATTCATCAAATTTCGCTCATTATGGCAATGCACACCAAGGCAACACTCAATTTTCACAATATGGTCAACATGGACAGCAAGCATACGGGCCAAGTTCTCAATGCCAATCCACCTCAAGTCAAGGATTTCATGCTCAAAATGCTCCTCCCGGGTTTACTCAAGGTGGTGGCTATGGTCCTAACAAGTTTCAAGGTGTTCCCATTGCCTCACAACCGGAGGAGAAGAAGAGTGCAAGTGATGATGCTCTTTCTTCTATGTCTCAATGCATTACTATACTATCCCAAGGTCAAGCCACCATGCAAAAACAAATGGGACTATTAGAAGTGCAATTGAGTCAAATGGCAAAGGAGCTTAGCACACGTCAACAAGGAGCCTTACCTTCACAACCGGAGCCAAACCCAAGAGGAAAACTCCATGAATGCAATGCGGTTACCACTCTACGTTCAGGAAAGGTATATGATAATCATGTTTACATGCCTACATCTTCTAGTCTCCATACAGATCCTTTATTTGATGATGATGTCGGTTTGCATTCATATGGGGCCAAGAGGGAAGAAAATGTACCTTTTGGCCATGTTGTTGATGACCAATTGCTTACACATCATGATTCTTTTTATCATAAGGAGGATGGGACCGGAAGGGAAGAAAATGTACCTTCCGGTAAATATGGAGTGGGTGATGTGACTTTGAATATCAAAGGAAGAAATAAGGGGGAGGATAAGGCAGCCGGAGGGGAAGAAAATGTACCTTCCGGAGGAGCTAATCTTGGTAAAGGTTATGGTTTCTTGTCCTTTGAGAACAATGCAAGCAAATATGGTTTGATTGATAGCGGTAAGGAAAGAGCTATATTGAGAAAGAACACTTCTAGGGATGAGGATGTCTCGGACCCTAAATTAGATGGTGAGGCCATGGAGGCTAGGGACCCATCTAAGGCCAATGACACCTCTAGGAAGGTTCTTGATGACATTAGACCCACCACACATGAGTTTGAGCCATCTACTTCTAGAGAAAAAGAGGGGAGGAATAAGGAGGAAGATTTACATATGCATGGTAGCTCTAGGGATAGGAGTGGTCCGGTCTACTCATCACTAGGCGAGGCCTTGAGGGCTAAGAACCCTAGTGGGACTGTGACTACCCTTAGGGGCACCACCGGGAGTCTCACCTTTGATCCACCTCTTGATTTGAGCACACCGGAACCTCAACTTCCTTACCCTAATGTGCCAAGAAAGGAAAATGTGAAGAAGGGTGGGAAGAAGAAAAAAATTGGCTTGATGTCCGAAGTGCATGATATTTTGAAGAAGGTCAATGTCAATATACCTTTGATTGAGCTCCTTCGACAAATGCCGGTTTATGCAAAATTTCTAAAGGACTTGTGCACCCATAAGAGGAAGATAAAGAATGATGAGCGGTTCGAGATTTGTGAGGAGGTTAGTGCTATCTTGCAAAGACGGATTCCCCCAAAACTCAAGGATCCGGGGAGCTTTAATATCTCTTGCACTATTGGTGAGAAAGTCTTTGATAGAGCTTTGATGGACCTTGGTTCAAGTGTTAACATTATTTCTTTTGAGACTTTCCGGAAACTTGACCTTGGACCTATTAAAGCTACTCCTATATTCTTGCAACTTGCGGATAGGAGTATTAAGAGAGCCATGGGTGTTGTTGAAGATGTGTTAATCAAGGTTGATAAGTTCTACTTGCCGGCGGATTTGGTGGTATTAGATATGGATGATGGACCTCATAGTGACAAAGACATACCTATCATTCTTGGTAGACCCTTTATGGCAACCGCGGGAATAAAAATTAATGTTCAAAAGGGCACCATCAAAATGAAGGTATTGGGAGAAAAGGTATTACTTAAGAGTCTTGAACCCATATATCCTCCGGAAATAGTGAAGAGTATGCTCTCTATCAATTTAGTGAAGGGTAATGCAGCCAAGAGTGAGCGGATCTTTGGACCTTTGAATCTCTCCAAGCCTACAATCCGTGCACAAAATCATTTCTCACCTATTTGGGGTTATGACAATGAGCCACCATTGAAGCATGATGATGTAGTTCCTACTTCTACTTTGGAGCCTACTATCGAGCTTGAAAAGCTAACAACCCCTATTCTTGAGACTTATGAGCAACACGAGAAGAAGAGTCCACCATTGAGTGAGGCACAAAAGGAGTCCAACAAGAAGTTAAGGGAGGTTTGTGGAGTTGTCAAGAAGGAAGGATCTAAGAGGTATGATTGGCCTCCACCTTCAAGTTACAAACTTGCTTCTTCAATTGCTAGATGTTTTGGAAATGATAGTGATGCTAGCACAATTGTTGTGGGTGGTAAGAAGACATCTTTTGAGCCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

979

Amino Acids

108.31

Weight (kDa)

7.65

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 453, 1736, 2408
AccBSI CCGCTC 2 cut(s) 1999, 2536
AccI GTMKAC 3 cut(s) 127, 1671, 2362
AccII CGCG 1 cut(s) 2381
AccIII TCCGGA 4 cut(s) 406, 1343, 2166, 2473
AclWI GGATC 8 cut(s) 176, 461, 1088, 1754, 2057, 2070, 2546, 2815
AcoI YGGCCR 2 cut(s) 580, 1162
AcsI RAATTY 3 cut(s) 439, 601, 1949
AfaI GTAC 3 cut(s) 1155, 1245, 1338
AfiI CCNNNNNNNGG 6 cut(s) 102, 249, 1346, 1522, 1977, 2603
AflII CTTAAG 1 cut(s) 2447
AflIII ACRYGT 1 cut(s) 950
AhlI ACTAGT 1 cut(s) 377
AjiI CACGTC 1 cut(s) 953
Alw21I GWGCWC 7 cut(s) 120, 206, 236, 1781, 1923, 1970, 2581
Alw26I GTCTC 8 cut(s) 206, 519, 855, 1088, 1477, 1754, 2153, 2699
Alw44I GTGCAC 2 cut(s) 1966, 2577
AlwI GGATC 8 cut(s) 176, 461, 1088, 1754, 2057, 2070, 2546, 2815
Ama87I CYCGRG 1 cut(s) 740
Aor13HI TCCGGA 4 cut(s) 406, 1343, 2166, 2473
AoxI GGCC 9 cut(s) 321, 580, 680, 1134, 1162, 1497, 1524, 1689, 2825
ApaLI GTGCAC 2 cut(s) 1966, 2577
ApeKI GCWGC 2 cut(s) 1316, 2522
ApoI RAATTY 3 cut(s) 439, 601, 1949
AseI ATTAAT 1 cut(s) 2394
AsuC2I CCSGG 5 cut(s) 492, 741, 742, 1744, 2067
AsuHPI GGTGA 8 cut(s) 354, 468, 497, 1280, 1505, 1744, 2108, 2586
AsuNHI GCTAGC 1 cut(s) 2891
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 9 cut(s) 767, 1224, 1477, 1513, 1664, 2125, 2183, 2324, 2546
AxyI CCTNAGG 1 cut(s) 1730
BaeGI GKGCMC 5 cut(s) 456, 1739, 1970, 2411, 2581
BalI TGGCCA 2 cut(s) 582, 1164
BamHI GGATCC 1 cut(s) 2062
BanI GGYRCC 3 cut(s) 453, 1736, 2408
BanII GRGCYC 2 cut(s) 236, 1923
BarI GAAGNNNNNNTAC 6 cut(s) 1137, 1169, 1227, 1259, 1320, 1352
BauI CACGAG 1 cut(s) 2720
BbsI GAAGAC 3 cut(s) 327, 341, 2924
Bbv12I GWGCWC 7 cut(s) 120, 206, 236, 1781, 1923, 1970, 2581
BbvI GCAGC 2 cut(s) 1328, 2534
BccI CCATC 7 cut(s) 1214, 1484, 1525, 1594, 2116, 2315, 2420
BceAI ACGGC 1 cut(s) 419
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 5 cut(s) 492, 741, 742, 1744, 2067
BcoDI GTCTC 8 cut(s) 206, 519, 855, 1088, 1477, 1754, 2153, 2699
BcuI ACTAGT 1 cut(s) 377
BfrI CTTAAG 1 cut(s) 2447
BglI GCCNNNNNGGC 2 cut(s) 1505, 1697
BisI GCNGC 4 cut(s) 63, 324, 1317, 2523
BlpI GCTNAGC 1 cut(s) 944
BlsI GCNGC 4 cut(s) 64, 325, 1318, 2524
Bme1390I CCNGG 5 cut(s) 492, 741, 742, 1744, 2067
Bme18I GGWCC 9 cut(s) 767, 1224, 1477, 1513, 1664, 2125, 2183, 2324, 2546
BmeT110I CYCGRG 1 cut(s) 740
BmgBI CACGTC 1 cut(s) 953
BmrFI CCNGG 5 cut(s) 492, 741, 742, 1744, 2067
BmsI GCATC 2 cut(s) 823, 2878
BmtI GCTAGC 1 cut(s) 2895
BpiI GAAGAC 3 cut(s) 327, 341, 2924
BplI GAGNNNNNCTC 4 cut(s) 987, 1019, 1457, 1489
Bpu1102I GCTNAGC 1 cut(s) 944
BpuEI CTTGAG 8 cut(s) 134, 273, 278, 691, 735, 1714, 2042, 2723
BpuMI CCSGG 5 cut(s) 492, 741, 742, 1744, 2067
BsaI GGTCTC 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 2424, 2454
Bsc4I CCNNNNNNNGG 6 cut(s) 102, 249, 1346, 1522, 1977, 2603
Bse118I RCCGGY 2 cut(s) 1936, 2290
Bse21I CCTNAGG 1 cut(s) 1730
Bse3DI GCAATG 4 cut(s) 146, 625, 792, 1021
BseAI TCCGGA 4 cut(s) 406, 1343, 2166, 2473
BseGI GGATG 5 cut(s) 151, 1225, 1468, 1474, 2323
BseLI CCNNNNNNNGG 6 cut(s) 102, 249, 1346, 1522, 1977, 2603
BseMI GCAATG 4 cut(s) 146, 625, 792, 1021
BseRI GAGGAG 6 cut(s) 58, 61, 726, 824, 1362, 2030
BseSI GKGCMC 5 cut(s) 456, 1739, 1970, 2411, 2581
BseXI GCAGC 2 cut(s) 1328, 2534
Bsh1236I CGCG 1 cut(s) 2381
BshFI GGCC 9 cut(s) 323, 582, 682, 1136, 1164, 1499, 1526, 1691, 2827
BshNI GGYRCC 3 cut(s) 453, 1736, 2408
BsiHKAI GWGCWC 7 cut(s) 120, 206, 236, 1781, 1923, 1970, 2581
BsiHKCI CYCGRG 1 cut(s) 740
BslFI GGGAC 4 cut(s) 918, 1237, 1526, 1729
BslI CCNNNNNNNGG 6 cut(s) 102, 249, 1346, 1522, 1977, 2603
BsmAI GTCTC 8 cut(s) 206, 519, 855, 1088, 1477, 1754, 2153, 2699
BsmFI GGGAC 4 cut(s) 918, 1237, 1526, 1729
BsmI GAATGC 2 cut(s) 1016, 1123
BsnI GGCC 9 cut(s) 323, 582, 682, 1136, 1164, 1499, 1526, 1691, 2827
Bso31I GGTCTC 1 cut(s) 519
BsoBI CYCGRG 1 cut(s) 740
Bsp13I TCCGGA 4 cut(s) 406, 1343, 2166, 2473
Bsp143I GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
Bsp1720I GCTNAGC 1 cut(s) 944
Bsp19I CCATGG 2 cut(s) 1500, 2241
BspANI GGCC 9 cut(s) 323, 582, 682, 1136, 1164, 1499, 1526, 1691, 2827
BspEI TCCGGA 4 cut(s) 406, 1343, 2166, 2473
BspFNI CGCG 1 cut(s) 2381
BspHI TCATGA 3 cut(s) 151, 208, 1195
BspOI GCTAGC 1 cut(s) 2895
BspPI GGATC 8 cut(s) 176, 461, 1088, 1754, 2057, 2070, 2546, 2815
BspT107I GGYRCC 3 cut(s) 453, 1736, 2408
BspTI CTTAAG 1 cut(s) 2447
BspTNI GGTCTC 1 cut(s) 519
BsrBI CCGCTC 2 cut(s) 1999, 2536
BsrDI GCAATG 4 cut(s) 146, 625, 792, 1021
BsrFI RCCGGY 2 cut(s) 1936, 2290
BssAI RCCGGY 2 cut(s) 1936, 2290
BssMI GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
BssSI CACGAG 1 cut(s) 2720
BssT1I CCWWGG 8 cut(s) 529, 583, 628, 874, 1500, 2128, 2179, 2241
Bst2BI CACGAG 1 cut(s) 2720
Bst4CI ACNGT 1 cut(s) 1720
Bst6I CTCTTC 3 cut(s) 812, 2480, 2723
BstAFI CTTAAG 1 cut(s) 2447
BstC8I GCNNGC 4 cut(s) 672, 1399, 2292, 2893
BstDEI CTNAG 5 cut(s) 944, 1521, 1701, 1730, 2811
BstDSI CCRYGG 3 cut(s) 1500, 2241, 2379
BstEII GGTNACC 1 cut(s) 1021
BstENI CCTNNNNNAGG 1 cut(s) 1344
BstF5I GGATG 5 cut(s) 151, 1225, 1468, 1474, 2323
BstFNI CGCG 1 cut(s) 2381
BstKTI GATC 8 cut(s) 151, 184, 469, 1096, 1762, 2065, 2541, 2810
BstMAI GTCTC 8 cut(s) 206, 519, 855, 1088, 1477, 1754, 2153, 2699
BstMBI GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
BstMWI GCNNNNNNNGC 4 cut(s) 731, 1505, 1697, 2683
BstNSI RCATGY 2 cut(s) 374, 1069
BstPI GGTNACC 1 cut(s) 1021
BstSCI CCNGG 5 cut(s) 490, 739, 740, 1742, 2065
BstSLI GKGCMC 5 cut(s) 456, 1739, 1970, 2411, 2581
BstUI CGCG 1 cut(s) 2381
BstV1I GCAGC 2 cut(s) 1328, 2534
BstV2I GAAGAC 3 cut(s) 327, 341, 2924
BstX2I RGATCY 5 cut(s) 181, 1093, 2062, 2538, 2807
BstYI RGATCY 5 cut(s) 181, 1093, 2062, 2538, 2807
Bsu36I CCTNAGG 1 cut(s) 1730
BsuRI GGCC 9 cut(s) 323, 582, 682, 1136, 1164, 1499, 1526, 1691, 2827
BtgI CCRYGG 3 cut(s) 1500, 2241, 2379
BtrI CACGTC 1 cut(s) 953
BtsCI GGATG 5 cut(s) 151, 1225, 1468, 1474, 2323
Cac8I GCNNGC 4 cut(s) 672, 1399, 2292, 2893
CciI TCATGA 3 cut(s) 151, 208, 1195
Cfr10I RCCGGY 2 cut(s) 1936, 2290
Cfr42I CCGCGG 1 cut(s) 2382
Cfr9I CCCGGG 1 cut(s) 740
Csp6I GTAC 3 cut(s) 1154, 1244, 1337
CspCI CAANNNNNGTGG 2 cut(s) 2886, 2921
CviQI GTAC 3 cut(s) 1154, 1244, 1337
DdeI CTNAG 5 cut(s) 944, 1521, 1701, 1730, 2811
DpnI GATC 8 cut(s) 150, 183, 468, 1095, 1761, 2064, 2540, 2809
DpnII GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
EaeI YGGCCR 2 cut(s) 580, 1162
Eam1104I CTCTTC 3 cut(s) 812, 2480, 2723
EarI CTCTTC 3 cut(s) 812, 2480, 2723
EciI GGCGGA 1 cut(s) 2309
Ecl136II GAGCTC 2 cut(s) 234, 1921
Eco130I CCWWGG 8 cut(s) 529, 583, 628, 874, 1500, 2128, 2179, 2241
Eco147I AGGCCT 1 cut(s) 1691
Eco24I GRGCYC 2 cut(s) 236, 1923
Eco31I GGTCTC 1 cut(s) 519
Eco47I GGWCC 9 cut(s) 767, 1224, 1477, 1513, 1664, 2125, 2183, 2324, 2546
Eco53kI GAGCTC 2 cut(s) 234, 1921
Eco81I CCTNAGG 1 cut(s) 1730
Eco88I CYCGRG 1 cut(s) 740
Eco91I GGTNACC 1 cut(s) 1021
EcoICRI GAGCTC 2 cut(s) 234, 1921
EcoNI CCTNNNNNAGG 1 cut(s) 1344
EcoO109I RGGNCCY 1 cut(s) 1513
EcoO65I GGTNACC 1 cut(s) 1021
EcoRI GAATTC 1 cut(s) 439
EcoT14I CCWWGG 8 cut(s) 529, 583, 628, 874, 1500, 2128, 2179, 2241
EcoT22I ATGCAT 2 cut(s) 860, 1641
EcoT38I GRGCYC 2 cut(s) 236, 1923
ErhI CCWWGG 8 cut(s) 529, 583, 628, 874, 1500, 2128, 2179, 2241
FalI AAGNNNNNCTT 6 cut(s) 60, 92, 554, 586, 2270, 2302
FaqI GGGAC 4 cut(s) 918, 1237, 1526, 1729
FauI CCCGC 1 cut(s) 2374
FauNDI CATATG 2 cut(s) 1129, 1635
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 3 cut(s) 127, 1671, 2362
Fnu4HI GCNGC 4 cut(s) 63, 324, 1317, 2523
FokI GGATG 5 cut(s) 158, 1232, 1475, 1481, 2330
FriOI GRGCYC 2 cut(s) 236, 1923
Fsp4HI GCNGC 4 cut(s) 63, 324, 1317, 2523
GluI GCNGC 4 cut(s) 63, 324, 1317, 2523
HaeIII GGCC 9 cut(s) 323, 582, 682, 1136, 1164, 1499, 1526, 1691, 2827
HincII GTYRAC 4 cut(s) 128, 659, 956, 2143
HindII GTYRAC 4 cut(s) 128, 659, 956, 2143
HindIII AAGCTT 3 cut(s) 254, 266, 568
HpaI GTTAAC 1 cut(s) 2143
HphI GGTGA 8 cut(s) 354, 468, 497, 1280, 1505, 1744, 2108, 2586
Hpy188I TCNGA 2 cut(s) 1477, 1870
HpyCH4III ACNGT 1 cut(s) 1720
HpyCH4IV ACGT 2 cut(s) 952, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 731, 1505, 1697, 2683
HpyF3I CTNAG 5 cut(s) 944, 1521, 1701, 1730, 2811
HpySE526I ACGT 2 cut(s) 952, 1033
KflI GGGWCCC 1 cut(s) 1513
Kpn2I TCCGGA 4 cut(s) 406, 1343, 2166, 2473
KroI GCCGGC 1 cut(s) 2290
KroNI GCCGGC 1 cut(s) 2292
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 2143
KspI CCGCGG 1 cut(s) 2382
Kzo9I GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
LmnI GCTCC 9 cut(s) 400, 739, 940, 962, 982, 1349, 1926, 2070, 2662
Lsp1109I GCAGC 2 cut(s) 1328, 2534
LweI GCATC 2 cut(s) 823, 2878
MaeII ACGT 2 cut(s) 952, 1033
MaeIII GTNAC 6 cut(s) 30, 1021, 1273, 1720, 2333, 2840
MalI GATC 8 cut(s) 150, 183, 468, 1095, 1761, 2064, 2540, 2809
MbiI CCGCTC 2 cut(s) 1999, 2536
MboI GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
MfeI CAATTG 4 cut(s) 920, 1181, 2859, 2898
MflI RGATCY 5 cut(s) 181, 1093, 2062, 2538, 2807
MlsI TGGCCA 2 cut(s) 582, 1164
MluNI TGGCCA 2 cut(s) 582, 1164
MlyI GAGTC 5 cut(s) 934, 1756, 2461, 2740, 2768
MmeI TCCRAC 2 cut(s) 476, 2787
Mox20I TGGCCA 2 cut(s) 582, 1164
Mph1103I ATGCAT 2 cut(s) 860, 1641
MroI TCCGGA 4 cut(s) 406, 1343, 2166, 2473
MroNI GCCGGC 1 cut(s) 2290
MscI TGGCCA 2 cut(s) 582, 1164
MseI TTAA 8 cut(s) 2076, 2142, 2190, 2232, 2264, 2394, 2448, 2774
MslI CAYNNNNRTG 6 cut(s) 84, 343, 1128, 1638, 2417, 2637
Msp20I TGGCCA 2 cut(s) 582, 1164
MspA1I CMGCKG 1 cut(s) 2381
MspCI CTTAAG 1 cut(s) 2447
MspR9I CCNGG 5 cut(s) 492, 741, 742, 1744, 2067
MunI CAATTG 4 cut(s) 920, 1181, 2859, 2898
Mva1269I GAATGC 2 cut(s) 1016, 1123
MvnI CGCG 1 cut(s) 2381
MwoI GCNNNNNNNGC 4 cut(s) 731, 1505, 1697, 2683
NaeI GCCGGC 1 cut(s) 2292
NciI CCSGG 5 cut(s) 492, 741, 742, 1744, 2067
NcoI CCATGG 2 cut(s) 1500, 2241
NdeI CATATG 2 cut(s) 1129, 1635
NdeII GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
NgoMIV GCCGGC 1 cut(s) 2290
NheI GCTAGC 1 cut(s) 2891
NmuCI GTSAC 3 cut(s) 1273, 1720, 2333
NsiI ATGCAT 2 cut(s) 860, 1641
NspI RCATGY 2 cut(s) 374, 1069
PagI TCATGA 3 cut(s) 151, 208, 1195
PceI AGGCCT 1 cut(s) 1691
PctI GAATGC 2 cut(s) 1016, 1123
PdiI GCCGGC 1 cut(s) 2292
PfeI GAWTC 7 cut(s) 155, 359, 502, 593, 1199, 2044, 2554
PkrI GCNGC 4 cut(s) 64, 325, 1318, 2524
PleI GAGTC 5 cut(s) 933, 1755, 2460, 2739, 2767
PpsI GAGTC 5 cut(s) 933, 1755, 2460, 2739, 2767
PpuMI RGGWCCY 1 cut(s) 1513
PshBI ATTAAT 1 cut(s) 2394
Psp124BI GAGCTC 2 cut(s) 236, 1923
Psp5II RGGWCCY 1 cut(s) 1513
PspEI GGTNACC 1 cut(s) 1021
PspPPI RGGWCCY 1 cut(s) 1513
PsuI RGATCY 5 cut(s) 181, 1093, 2062, 2538, 2807
RsaI GTAC 3 cut(s) 1155, 1245, 1338
RsaNI GTAC 3 cut(s) 1154, 1244, 1337
RseI CAYNNNNRTG 6 cut(s) 84, 343, 1128, 1638, 2417, 2637
SacI GAGCTC 2 cut(s) 236, 1923
SacII CCGCGG 1 cut(s) 2382
SalI GTCGAC 1 cut(s) 126
SaqAI TTAA 8 cut(s) 2076, 2142, 2190, 2232, 2264, 2394, 2448, 2774
SatI GCNGC 4 cut(s) 63, 324, 1317, 2523
Sau3AI GATC 8 cut(s) 148, 181, 466, 1093, 1759, 2062, 2538, 2807
SchI GAGTC 5 cut(s) 934, 1756, 2461, 2740, 2768
ScrFI CCNGG 5 cut(s) 492, 741, 742, 1744, 2067
SfaNI GCATC 2 cut(s) 823, 2878
Sfr303I CCGCGG 1 cut(s) 2382
SgrBI CCGCGG 1 cut(s) 2382
SinI GGWCC 9 cut(s) 767, 1224, 1477, 1513, 1664, 2125, 2183, 2324, 2546
SmaI CCCGGG 1 cut(s) 742
SmiMI CAYNNNNRTG 6 cut(s) 84, 343, 1128, 1638, 2417, 2637
SmlI CTYRAG 9 cut(s) 113, 257, 288, 706, 750, 1693, 2057, 2447, 2702
SmoI CTYRAG 9 cut(s) 113, 257, 288, 706, 750, 1693, 2057, 2447, 2702
SpeI ACTAGT 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 1691
SstI GAGCTC 2 cut(s) 236, 1923
StuI AGGCCT 1 cut(s) 1691
StyD4I CCNGG 5 cut(s) 490, 739, 740, 1742, 2065
StyI CCWWGG 8 cut(s) 529, 583, 628, 874, 1500, 2128, 2179, 2241
TaaI ACNGT 1 cut(s) 1720
TaiI ACGT 2 cut(s) 955, 1036
TaqI TCGA 5 cut(s) 127, 134, 1927, 2004, 2673
TauI GCSGC 2 cut(s) 65, 326
TfiI GAWTC 7 cut(s) 155, 359, 502, 593, 1199, 2044, 2554
Tru1I TTAA 8 cut(s) 2076, 2142, 2190, 2232, 2264, 2394, 2448, 2774
Tru9I TTAA 8 cut(s) 2076, 2142, 2190, 2232, 2264, 2394, 2448, 2774
TseFI GTSAC 3 cut(s) 1273, 1720, 2333
TseI GCWGC 2 cut(s) 1316, 2522
Tsp45I GTSAC 3 cut(s) 1273, 1720, 2333
TspDTI ATGAA 9 cut(s) 68, 291, 327, 528, 585, 710, 1023, 1116, 2435
TspGWI ACGGA 2 cut(s) 2056, 2564
TspMI CCCGGG 1 cut(s) 740
Vha464I CTTAAG 1 cut(s) 2447
VneI GTGCAC 2 cut(s) 1966, 2577
VpaK11BI GGWCC 9 cut(s) 767, 1224, 1477, 1513, 1664, 2125, 2183, 2324, 2546
VspI ATTAAT 1 cut(s) 2394
XagI CCTNNNNNAGG 1 cut(s) 1344
XapI RAATTY 3 cut(s) 439, 601, 1949
XbaI TCTAGA 1 cut(s) 1595
XceI RCATGY 2 cut(s) 374, 1069
XmaI CCCGGG 1 cut(s) 740
XmiI GTMKAC 3 cut(s) 127, 1671, 2362
Zsp2I ATGCAT 2 cut(s) 860, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.