Rmu_sc0003527.1_g000011

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003527.1
OG0001407
Physical Location & Seq
Forward (+)
39566 .. 40696
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003527.1_g000011.1.cds

Sequence Viewer

Length: 1131 bp
atggccaccaatatgagtgagagaccaaaaggagccttaccttcacaactagagccaaacccaagaggaaaactccatgattgcaatgccatcaccactctacgttcgggaaaggtatatgataatcatgtttacatgcctacattttctagtttccatacaaatcctttatttgatgacgatgttgatttgcattcatattgggccaagagggaagaaaatgtacctcttggacatgtttttgatgatcaattgcatacacatcatgagtctttttatcataaggaggatgggaccggaagggaagaaaatgtaccttccggtaaatatggagtgggtgatgtgactttgaatgccaaaggaagaaataagggggtggataaggtagccggaggggaagaaaatgtacccttcggagaatctattcttggtaaaggttatggtttcttgtcctttgagaacaatgcaagtaaatatgggttgattaataatggcaaggaaagagctttgttgagaaagacttctagggatgaggatgtcttggaccctaaattagatggtgaggccatgaaggctagggacccatctacggccgataacacctctaggaaggttcttgatgacattagacccaccactcatgagtttgagccatctacttctagagacagagaggggaggaataaggaggaagatcaacatatgcatggtagctttagggatatgagtggtccggtctactcatcactaggcgagaccttgagggctaagaaccctagtgggaccgtgactacccttaggggcactaccggaaatctcacatttgatccacctcttgatttgaatgtaccggagccaccacttccttttcctaatgccgcaagaagagataactcaaagaggggtaagaagaagagtggcttgatgagggaaatacaagaaatatttaagaaagtggatgtcaatatcccattgatggacctcattcaacaagtgccggtgtatgctcaatttctcaagagcttgtgcatgaataaaaagaaatttaaagccgatgagacatttgagttgaaggaggaagtaagtgcaattatccaccaaagatgcaagatccggggagctttaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 ko04210 ko04212 ko04214 ko04217 ko05010 map03410 map04210 map04212 map04214 map04217 map05010
Pfam Domains
Protein Families
Exo_endo_phos_2 RVT_1 RVT_3 zf-RVT

Protein Analysis

376

Amino Acids

42.16

Weight (kDa)

6.91

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 738
AciI CCGC 1 cut(s) 879
AclWI GGATC 2 cut(s) 821, 1105
AcoI YGGCCR 2 cut(s) 3, 591
AcsI RAATTY 1 cut(s) 1043
AfaI GTAC 4 cut(s) 225, 315, 408, 849
AfiI CCNNNNNNNGG 2 cut(s) 589, 976
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 4 cut(s) 352, 844, 989, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 411, 443
AluBI AGCT 4 cut(s) 506, 714, 1023, 1121
AluI AGCT 4 cut(s) 506, 714, 1023, 1121
Alw26I GTCTC 4 cut(s) 16, 660, 749, 1052
AlwI GGATC 2 cut(s) 821, 1105
AoxI GGCC 4 cut(s) 3, 204, 564, 591
ApoI RAATTY 1 cut(s) 1043
AseI ATTAAT 1 cut(s) 486
Asp700I GAANNNNTTC 2 cut(s) 423, 520
AspS9I GGNCC 7 cut(s) 204, 294, 544, 580, 731, 783, 979
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 3 cut(s) 85, 350, 572
AvaII GGWCC 6 cut(s) 294, 544, 580, 731, 783, 979
AxyI CCTNAGG 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 806
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 6 cut(s) 207, 239, 297, 329, 390, 422
BccI CCATC 6 cut(s) 98, 284, 551, 592, 661, 970
BceAI ACGGC 1 cut(s) 606
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 4 cut(s) 16, 660, 749, 1052
BfaI CTAG 8 cut(s) 50, 150, 525, 576, 606, 663, 749, 777
BglI GCCNNNNNGGC 1 cut(s) 572
BisI GCNGC 1 cut(s) 879
BlsI GCNGC 1 cut(s) 880
Bme1390I CCNGG 1 cut(s) 1115
Bme18I GGWCC 6 cut(s) 294, 544, 580, 731, 783, 979
BmgT120I GGNCC 7 cut(s) 204, 294, 544, 580, 731, 783, 979
BmiI GGNNCC 7 cut(s) 34, 295, 546, 581, 582, 784, 855
BmrFI CCNGG 1 cut(s) 1115
BmsI GCATC 1 cut(s) 1094
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpuEI CTTGAG 2 cut(s) 781, 1001
BpuMI CCSGG 1 cut(s) 1115
BsaI GGTCTC 2 cut(s) 16, 749
BsaJI CCNNGG 1 cut(s) 1114
BsaWI WCCGGW 5 cut(s) 296, 320, 733, 809, 850
Bsc4I CCNNNNNNNGG 2 cut(s) 589, 976
Bse118I RCCGGY 1 cut(s) 997
Bse21I CCTNAGG 1 cut(s) 797
Bse3DI GCAATG 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 1114
BseGI GGATG 4 cut(s) 295, 535, 541, 964
BseLI CCNNNNNNNGG 2 cut(s) 589, 976
BseMI GCAATG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 806
BseX3I CGGCCG 1 cut(s) 591
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 4 cut(s) 5, 206, 566, 593
BsiEI CGRYCG 1 cut(s) 594
BsiSI CCGG 8 cut(s) 297, 321, 390, 734, 810, 851, 998, 1114
BslFI GGGAC 3 cut(s) 307, 593, 796
BslI CCNNNNNNNGG 2 cut(s) 589, 976
BsmAI GTCTC 4 cut(s) 16, 660, 749, 1052
BsmFI GGGAC 3 cut(s) 307, 593, 796
BsmI GAATGC 2 cut(s) 193, 358
BsnI GGCC 4 cut(s) 5, 206, 566, 593
Bso31I GGTCTC 2 cut(s) 16, 749
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 4 cut(s) 247, 694, 826, 1110
BspACI CCGC 1 cut(s) 879
BspANI GGCC 4 cut(s) 5, 206, 566, 593
BspHI TCATGA 2 cut(s) 265, 640
BspLI GGNNCC 7 cut(s) 34, 295, 546, 581, 582, 784, 855
BspPI GGATC 2 cut(s) 821, 1105
BspTNI GGTCTC 2 cut(s) 16, 749
BsrDI GCAATG 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 997
BssAI RCCGGY 1 cut(s) 997
BssECI CCNNGG 1 cut(s) 1114
BssMI GATC 4 cut(s) 247, 694, 826, 1110
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 2 cut(s) 880, 908
BstDEI CTNAG 2 cut(s) 768, 797
BstF5I GGATG 4 cut(s) 295, 535, 541, 964
BstKTI GATC 4 cut(s) 250, 697, 829, 1113
BstMAI GTCTC 4 cut(s) 16, 660, 749, 1052
BstMBI GATC 4 cut(s) 247, 694, 826, 1110
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 139, 239
BstSCI CCNGG 1 cut(s) 1113
BstSLI GKGCMC 1 cut(s) 806
BstX2I RGATCY 1 cut(s) 1110
BstYI RGATCY 1 cut(s) 1110
BstZI CGGCCG 1 cut(s) 591
Bsu36I CCTNAGG 1 cut(s) 797
BsuRI GGCC 4 cut(s) 5, 206, 566, 593
BtsCI GGATG 4 cut(s) 295, 535, 541, 964
CciI TCATGA 2 cut(s) 265, 640
Cfr10I RCCGGY 1 cut(s) 997
Cfr13I GGNCC 7 cut(s) 204, 294, 544, 580, 731, 783, 979
Csp6I GTAC 4 cut(s) 224, 314, 407, 848
CviAII CATG 9 cut(s) 77, 128, 136, 236, 266, 568, 641, 707, 1030
CviQI GTAC 4 cut(s) 224, 314, 407, 848
DdeI CTNAG 2 cut(s) 768, 797
DpnI GATC 4 cut(s) 249, 696, 828, 1112
DpnII GATC 4 cut(s) 247, 694, 826, 1110
DraI TTTAAA 1 cut(s) 1048
EaeI YGGCCR 2 cut(s) 3, 591
EagI CGGCCG 1 cut(s) 591
Eam1104I CTCTTC 2 cut(s) 880, 908
EarI CTCTTC 2 cut(s) 880, 908
EclXI CGGCCG 1 cut(s) 591
Eco31I GGTCTC 2 cut(s) 16, 749
Eco47I GGWCC 6 cut(s) 294, 544, 580, 731, 783, 979
Eco52I CGGCCG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 580
EcoT22I ATGCAT 1 cut(s) 708
FaeI CATG 9 cut(s) 80, 131, 139, 239, 269, 571, 644, 710, 1033
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FaqI GGGAC 3 cut(s) 307, 593, 796
FatI CATG 9 cut(s) 76, 127, 135, 235, 265, 567, 640, 706, 1029
FauNDI CATATG 1 cut(s) 702
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 1 cut(s) 879
FokI GGATG 4 cut(s) 302, 542, 548, 971
Fsp4HI GCNGC 1 cut(s) 879
FspBI CTAG 8 cut(s) 50, 150, 525, 576, 606, 663, 749, 777
GluI GCNGC 1 cut(s) 879
HaeIII GGCC 4 cut(s) 5, 206, 566, 593
HapII CCGG 8 cut(s) 297, 321, 390, 734, 810, 851, 998, 1114
Hin1II CATG 9 cut(s) 80, 131, 139, 239, 269, 571, 644, 710, 1033
HinfI GANTC 2 cut(s) 269, 419
HpaII CCGG 8 cut(s) 297, 321, 390, 734, 810, 851, 998, 1114
HphI GGTGA 3 cut(s) 85, 350, 572
Hpy166II GTNNAC 2 cut(s) 133, 739
Hpy188I TCNGA 1 cut(s) 416
Hpy188III TCNNGA 7 cut(s) 108, 266, 617, 641, 663, 836, 1018
Hpy8I GTNNAC 2 cut(s) 133, 739
HpyAV CCTTC 7 cut(s) 51, 294, 327, 421, 565, 604, 1066
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 8 cut(s) 84, 193, 256, 467, 706, 1029, 1088, 1107
HpyF10VI GCNNNNNNNGC 1 cut(s) 572
HpyF3I CTNAG 2 cut(s) 768, 797
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 9 cut(s) 80, 131, 139, 239, 269, 571, 644, 710, 1033
KflI GGGWCCC 1 cut(s) 580
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 4 cut(s) 247, 694, 826, 1110
LmnI GCTCC 3 cut(s) 32, 853, 1118
LpnPI CCDG 8 cut(s) 310, 334, 403, 747, 823, 864, 1011, 1127
LweI GCATC 1 cut(s) 1094
MaeI CTAG 8 cut(s) 50, 150, 525, 576, 606, 663, 749, 777
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 2 cut(s) 343, 787
MalI GATC 4 cut(s) 249, 696, 828, 1112
MboI GATC 4 cut(s) 247, 694, 826, 1110
MboII GAAGA 8 cut(s) 227, 317, 375, 410, 704, 897, 922, 925
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 1110
MhlI GDGCHC 1 cut(s) 806
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 251, 551, 1010, 1043, 1089
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 278
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 708
MroXI GAANNNNTTC 2 cut(s) 423, 520
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 486, 948, 1047
MslI CAYNNNNRTG 2 cut(s) 11, 705
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 8 cut(s) 297, 321, 390, 734, 810, 851, 998, 1114
MspR9I CCNGG 1 cut(s) 1115
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 2 cut(s) 193, 358
MwoI GCNNNNNNNGC 1 cut(s) 572
NciI CCSGG 1 cut(s) 1115
NdeI CATATG 1 cut(s) 702
NdeII GATC 4 cut(s) 247, 694, 826, 1110
NlaIII CATG 9 cut(s) 80, 131, 139, 239, 269, 571, 644, 710, 1033
NlaIV GGNNCC 7 cut(s) 34, 295, 546, 581, 582, 784, 855
NmuCI GTSAC 2 cut(s) 343, 787
NsiI ATGCAT 1 cut(s) 708
NspI RCATGY 2 cut(s) 139, 239
PagI TCATGA 2 cut(s) 265, 640
PciI ACATGT 1 cut(s) 235
PctI GAATGC 2 cut(s) 193, 358
PdmI GAANNNNTTC 2 cut(s) 423, 520
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 880
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 580
PscI ACATGT 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 580
PspN4I GGNNCC 7 cut(s) 34, 295, 546, 581, 582, 784, 855
PspPI GGNCC 7 cut(s) 204, 294, 544, 580, 731, 783, 979
PspPPI RGGWCCY 1 cut(s) 580
PsuI RGATCY 1 cut(s) 1110
RsaI GTAC 4 cut(s) 225, 315, 408, 849
RsaNI GTAC 4 cut(s) 224, 314, 407, 848
RseI CAYNNNNRTG 2 cut(s) 11, 705
SaqAI TTAA 3 cut(s) 486, 948, 1047
SatI GCNGC 1 cut(s) 879
Sau3AI GATC 4 cut(s) 247, 694, 826, 1110
Sau96I GGNCC 7 cut(s) 204, 294, 544, 580, 731, 783, 979
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 1115
SduI GDGCHC 1 cut(s) 806
SfaNI GCATC 1 cut(s) 1094
SinI GGWCC 6 cut(s) 294, 544, 580, 731, 783, 979
SmiMI CAYNNNNRTG 2 cut(s) 11, 705
SmlI CTYRAG 2 cut(s) 760, 1016
SmoI CTYRAG 2 cut(s) 760, 1016
Sse9I AATT 5 cut(s) 251, 551, 1010, 1043, 1089
SsiI CCGC 1 cut(s) 879
SspI AATATT 1 cut(s) 945
SspMI CTAG 8 cut(s) 50, 150, 525, 576, 606, 663, 749, 777
StyD4I CCNGG 1 cut(s) 1113
TaaI ACNGT 1 cut(s) 787
TaiI ACGT 1 cut(s) 106
TasI AATT 5 cut(s) 251, 551, 1010, 1043, 1089
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 3 cut(s) 486, 948, 1047
Tru9I TTAA 3 cut(s) 486, 948, 1047
TseFI GTSAC 2 cut(s) 343, 787
Tsp45I GTSAC 2 cut(s) 343, 787
TspDTI ATGAA 3 cut(s) 186, 584, 1046
VpaK11BI GGWCC 6 cut(s) 294, 544, 580, 731, 783, 979
VspI ATTAAT 1 cut(s) 486
XapI RAATTY 1 cut(s) 1043
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 2 cut(s) 139, 239
XmiI GTMKAC 1 cut(s) 738
XmnI GAANNNNTTC 2 cut(s) 423, 520
XspI CTAG 8 cut(s) 50, 150, 525, 576, 606, 663, 749, 777
Zsp2I ATGCAT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.