Rmu_sc0000677.1_g000017

Aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000677.1
OG0001407
Physical Location & Seq
Reverse (-)
101966 .. 104411
2446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000677.1_g000017.1.cds

Sequence Viewer

Length: 1230 bp
atgcatcgggtggcaattttcttaagaaatgtcccaatgccgcttgagatatttgtggggaagtggctacacaatccggtcaatgggataatcatcaaaggagaagaggaggtagagatgaccgcactacttctagaggtcgtgattcacatgcatatggtactagaggtcgtgatgattctatggagtctagaggctgagttgatatggagcctcgtgcgcctagcagcctcggccacccagccgcccaacccgcctaggcacccgcctaaatcatgtccgcccaatgcctccgattacccattactcaagtcagcctgctgctgcccagtgcctaggcggccgttttttagaacactggtcaagagtgttactacttcacatctttgcttgatttgtgagtccccttatcattctactcatgatagtcacttggcttcgcaatttccggaatttgttgagaagcaagttaaagcggttagttcttatcaaaggccaccaagaaatgatccttactctaccacatataatccaggttggcgtcatcacccaaatttctcatggagagatggtagagggtcctctaatacacaccaaggtgtcactcatatggagggaatcaaggccgccttaattcttcatcttttagtctccacggccatggtaacacacaatttggtgcacaaaattattttgggcagcaagcatctacttacaactctcaaggccaagggatatcttttagtgctacacatgcttatggaggagccccacatgacattcaaaggtttggtcaaagttcccaatcattcggagagcttggagcatgttcacaaggagccctaccttcacaaccggagccaaatccaagaggcaaagtccatgaatgcaatgccgttaccactttacgttcgggaaagggatgaggatatctcggcctcaagattagatggtgaggccatgaaggcgagggacccatctaattccgatgacacctctaggaaggttcttaatgatattagacccacatcgtatgagtttgagtcatctacttcaaccgaaaaagaggggagaaataaggaggaagacctacatgtgcatggtagctctagggataggagtggtctggtctactcatcactaggcgaggccttaagggctaagaaccctagtgggaccgtgactacctttaggggcacggattccgacaaagttgatcgaagaaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

46.25

Weight (kDa)

8.62

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 1131
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 9 cut(s) 41, 123, 245, 254, 266, 281, 340, 476, 627
AclWI GGATC 1 cut(s) 503
AcoI YGGCCR 3 cut(s) 234, 341, 657
AcsI RAATTY 2 cut(s) 452, 553
AcyI GRCGYC 1 cut(s) 541
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 1 cut(s) 83
AflII CTTAAG 2 cut(s) 22, 1153
AflIII ACRYGT 1 cut(s) 1093
AgsI TTSAA 2 cut(s) 784, 1056
AjnI CCWGG 1 cut(s) 532
AleI CACNNNNGTG 1 cut(s) 597
AluBI AGCT 2 cut(s) 819, 1107
AluI AGCT 2 cut(s) 819, 1107
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 1 cut(s) 655
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 1 cut(s) 503
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 9 cut(s) 234, 341, 494, 624, 657, 726, 936, 957, 1149
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 4 cut(s) 227, 321, 324, 699
ApoI RAATTY 2 cut(s) 452, 553
ArsI GACNNNNNNTTYG 2 cut(s) 777, 809
AspA2I CCTAGG 2 cut(s) 257, 335
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 3 cut(s) 579, 973, 1176
AsuHPI GGTGA 2 cut(s) 539, 965
AvaII GGWCC 3 cut(s) 579, 973, 1176
AvrII CCTAGG 2 cut(s) 257, 335
BaeGI GKGCMC 2 cut(s) 684, 1199
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 2 cut(s) 771, 843
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 1092
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 239, 308, 311, 711
BccI CCATC 3 cut(s) 563, 944, 985
BceAI ACGGC 3 cut(s) 328, 672, 880
BciT130I CCWGG 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 655
BfrI CTTAAG 2 cut(s) 22, 1153
BglI GCCNNNNNGGC 3 cut(s) 340, 965, 1157
BisI GCNGC 8 cut(s) 41, 228, 245, 322, 325, 341, 627, 700
BlnI CCTAGG 2 cut(s) 257, 335
BlsI GCNGC 8 cut(s) 42, 229, 246, 323, 326, 342, 628, 701
Bme1390I CCNGG 1 cut(s) 534
Bme18I GGWCC 3 cut(s) 579, 973, 1176
BmgT120I GGNCC 3 cut(s) 579, 973, 1176
BmiI GGNNCC 9 cut(s) 212, 263, 580, 768, 840, 860, 974, 975, 1177
BmrFI CCNGG 1 cut(s) 534
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 2 cut(s) 13, 715
BmuI ACTGGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 1092
BplI GAGNNNNNCTC 2 cut(s) 917, 949
BpuEI CTTGAG 4 cut(s) 65, 293, 707, 925
BsaBI GATNNNNATC 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 541
BsaJI CCNNGG 7 cut(s) 231, 257, 335, 595, 654, 660, 729
BsaWI WCCGGW 3 cut(s) 76, 448, 855
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 329, 363
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 92
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 7 cut(s) 231, 257, 335, 595, 654, 660, 729
BseGI GGATG 1 cut(s) 928
BseJI GATNNNNATC 1 cut(s) 92
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 329, 363
BseRI GAGGAG 2 cut(s) 122, 779
BseSI GKGCMC 2 cut(s) 684, 1199
BseX3I CGGCCG 1 cut(s) 341
BseXI GCAGC 4 cut(s) 239, 308, 311, 711
BseYI CCCAGC 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 9 cut(s) 236, 343, 496, 626, 659, 728, 938, 959, 1151
BshNI GGYRCC 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 3 cut(s) 77, 449, 856
BslFI GGGAC 4 cut(s) 17, 388, 986, 1189
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 655
BsmFI GGGAC 4 cut(s) 17, 388, 986, 1189
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 9 cut(s) 236, 343, 496, 626, 659, 728, 938, 959, 1151
Bsp1286I GDGCHC 4 cut(s) 684, 771, 843, 1199
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 508, 1216
Bsp19I CCATGG 1 cut(s) 660
BspACI CCGC 9 cut(s) 41, 123, 245, 254, 266, 281, 340, 476, 627
BspANI GGCC 9 cut(s) 236, 343, 496, 626, 659, 728, 938, 959, 1151
BspCNI CTCAG 1 cut(s) 190
BspEI TCCGGA 1 cut(s) 448
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 9 cut(s) 212, 263, 580, 768, 840, 860, 974, 975, 1177
BspPI GGATC 1 cut(s) 503
BspT107I GGYRCC 1 cut(s) 261
BspTI CTTAAG 2 cut(s) 22, 1153
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 2 cut(s) 329, 363
BssECI CCNNGG 7 cut(s) 231, 257, 335, 595, 654, 660, 729
BssMI GATC 2 cut(s) 508, 1216
BssNI GRCGYC 1 cut(s) 541
BssSI CACGAG 1 cut(s) 215
BssT1I CCWWGG 5 cut(s) 257, 335, 595, 660, 729
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 1 cut(s) 1180
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 1 cut(s) 541
BstAFI CTTAAG 2 cut(s) 22, 1153
BstC8I GCNNGC 2 cut(s) 319, 704
BstDEI CTNAG 2 cut(s) 198, 1161
BstDSI CCRYGG 2 cut(s) 654, 660
BstF5I GGATG 1 cut(s) 928
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 511, 1219
BstMAI GTCTC 1 cut(s) 655
BstMBI GATC 2 cut(s) 508, 1216
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 7 cut(s) 219, 233, 253, 340, 754, 965, 1157
BstNI CCWGG 1 cut(s) 534
BstNSI RCATGY 4 cut(s) 154, 757, 830, 1097
BstSCI CCNGG 1 cut(s) 532
BstSLI GKGCMC 2 cut(s) 684, 1199
BstV1I GCAGC 4 cut(s) 239, 308, 311, 711
BstV2I GAAGAC 1 cut(s) 1092
BstXI CCANNNNNNTGG 1 cut(s) 661
BstZI CGGCCG 1 cut(s) 341
BsuRI GGCC 9 cut(s) 236, 343, 496, 626, 659, 728, 938, 959, 1151
BtgI CCRYGG 2 cut(s) 654, 660
BtsCI GGATG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 336, 356
Cac8I GCNNGC 2 cut(s) 319, 704
CciI TCATGA 1 cut(s) 421
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 3 cut(s) 579, 973, 1176
CseI GACGC 1 cut(s) 530
Csp6I GTAC 1 cut(s) 161
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 2 cut(s) 198, 1161
DpnI GATC 2 cut(s) 510, 1218
DpnII GATC 2 cut(s) 508, 1216
EaeI YGGCCR 3 cut(s) 234, 341, 657
EagI CGGCCG 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 341
Eco130I CCWWGG 5 cut(s) 257, 335, 595, 660, 729
Eco147I AGGCCT 1 cut(s) 1151
Eco24I GRGCYC 2 cut(s) 771, 843
Eco32I GATATC 2 cut(s) 737, 931
Eco47I GGWCC 3 cut(s) 579, 973, 1176
Eco52I CGGCCG 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 579, 973
EcoRII CCWGG 1 cut(s) 532
EcoRV GATATC 2 cut(s) 737, 931
EcoT14I CCWWGG 5 cut(s) 257, 335, 595, 660, 729
EcoT22I ATGCAT 2 cut(s) 6, 156
EcoT38I GRGCYC 2 cut(s) 771, 843
ErhI CCWWGG 5 cut(s) 257, 335, 595, 660, 729
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 4 cut(s) 17, 388, 986, 1189
FauI CCCGC 2 cut(s) 261, 273
FauNDI CATATG 2 cut(s) 156, 609
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 8 cut(s) 41, 228, 245, 322, 325, 341, 627, 700
FokI GGATG 1 cut(s) 935
FriOI GRGCYC 2 cut(s) 771, 843
Fsp4HI GCNGC 8 cut(s) 41, 228, 245, 322, 325, 341, 627, 700
GlaI GCGC 1 cut(s) 221
GluI GCNGC 8 cut(s) 41, 228, 245, 322, 325, 341, 627, 700
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 9 cut(s) 236, 343, 496, 626, 659, 728, 938, 959, 1151
HapII CCGG 3 cut(s) 77, 449, 856
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 541
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 7 cut(s) 145, 178, 187, 401, 618, 1043, 1202
HpaII CCGG 3 cut(s) 77, 449, 856
HphI GGTGA 2 cut(s) 539, 965
Hpy166II GTNNAC 3 cut(s) 682, 832, 1132
Hpy188I TCNGA 4 cut(s) 295, 814, 988, 1207
Hpy188III TCNNGA 9 cut(s) 134, 142, 172, 191, 364, 422, 449, 914, 942
Hpy8I GTNNAC 3 cut(s) 682, 832, 1132
HpyAV CCTTC 3 cut(s) 857, 958, 997
HpyCH4III ACNGT 1 cut(s) 1180
HpyCH4IV ACGT 1 cut(s) 909
HpyCH4V TGCA 5 cut(s) 4, 154, 682, 890, 1099
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 233, 253, 340, 754, 965, 1157
HpyF3I CTNAG 2 cut(s) 198, 1161
HpySE526I ACGT 1 cut(s) 909
Hsp92I GRCGYC 1 cut(s) 541
HspAI GCGC 1 cut(s) 220
KflI GGGWCCC 1 cut(s) 973
Kpn2I TCCGGA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 508, 1216
LmnI GCTCC 5 cut(s) 210, 766, 823, 838, 858
Lsp1109I GCAGC 4 cut(s) 239, 308, 311, 711
LweI GCATC 2 cut(s) 13, 715
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 6 cut(s) 370, 428, 601, 664, 897, 1180
MalI GATC 2 cut(s) 510, 1218
MboI GATC 2 cut(s) 508, 1216
MboII GAAGA 3 cut(s) 116, 629, 1097
MhlI GDGCHC 4 cut(s) 684, 771, 843, 1199
MluCI AATT 8 cut(s) 15, 443, 452, 553, 633, 673, 687, 982
MlyI GAGTC 3 cut(s) 196, 410, 1052
MmeI TCCRAC 1 cut(s) 1230
Mph1103I ATGCAT 2 cut(s) 6, 156
MroI TCCGGA 1 cut(s) 448
MseI TTAA 5 cut(s) 23, 471, 632, 1011, 1154
MslI CAYNNNNRTG 6 cut(s) 155, 597, 608, 659, 758, 1098
MspCI CTTAAG 2 cut(s) 22, 1153
MspI CCGG 3 cut(s) 77, 449, 856
MspR9I CCNGG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 7 cut(s) 219, 233, 253, 340, 754, 965, 1157
NcoI CCATGG 1 cut(s) 660
NdeI CATATG 2 cut(s) 156, 609
NdeII GATC 2 cut(s) 508, 1216
NlaIV GGNNCC 9 cut(s) 212, 263, 580, 768, 840, 860, 974, 975, 1177
NmeAIII GCCGAG 2 cut(s) 212, 914
NmuCI GTSAC 3 cut(s) 428, 601, 1180
NsiI ATGCAT 2 cut(s) 6, 156
NspI RCATGY 4 cut(s) 154, 757, 830, 1097
OliI CACNNNNGTG 1 cut(s) 597
PagI TCATGA 1 cut(s) 421
PceI AGGCCT 1 cut(s) 1151
PciI ACATGT 1 cut(s) 1093
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 4 cut(s) 145, 178, 618, 1202
PkrI GCNGC 8 cut(s) 42, 229, 246, 323, 326, 342, 628, 701
PleI GAGTC 3 cut(s) 195, 409, 1051
PpsI GAGTC 3 cut(s) 195, 409, 1051
PpuMI RGGWCCY 2 cut(s) 579, 973
PscI ACATGT 1 cut(s) 1093
Psp5II RGGWCCY 2 cut(s) 579, 973
Psp6I CCWGG 1 cut(s) 532
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 9 cut(s) 212, 263, 580, 768, 840, 860, 974, 975, 1177
PspPI GGNCC 3 cut(s) 579, 973, 1176
PspPPI RGGWCCY 2 cut(s) 579, 973
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
RseI CAYNNNNRTG 6 cut(s) 155, 597, 608, 659, 758, 1098
SaqAI TTAA 5 cut(s) 23, 471, 632, 1011, 1154
SatI GCNGC 8 cut(s) 41, 228, 245, 322, 325, 341, 627, 700
Sau3AI GATC 2 cut(s) 508, 1216
Sau96I GGNCC 3 cut(s) 579, 973, 1176
SchI GAGTC 3 cut(s) 196, 410, 1052
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 4 cut(s) 684, 771, 843, 1199
SfaNI GCATC 2 cut(s) 13, 715
SinI GGWCC 3 cut(s) 579, 973, 1176
SmiMI CAYNNNNRTG 6 cut(s) 155, 597, 608, 659, 758, 1098
SmlI CTYRAG 6 cut(s) 22, 44, 308, 722, 940, 1153
SmoI CTYRAG 6 cut(s) 22, 44, 308, 722, 940, 1153
Sse9I AATT 8 cut(s) 15, 443, 452, 553, 633, 673, 687, 982
SseBI AGGCCT 1 cut(s) 1151
SsiI CCGC 9 cut(s) 41, 123, 245, 254, 266, 281, 340, 476, 627
StuI AGGCCT 1 cut(s) 1151
StyD4I CCNGG 1 cut(s) 532
StyI CCWWGG 5 cut(s) 257, 335, 595, 660, 729
TaaI ACNGT 1 cut(s) 1180
TaiI ACGT 1 cut(s) 912
TaqI TCGA 1 cut(s) 1219
TasI AATT 8 cut(s) 15, 443, 452, 553, 633, 673, 687, 982
TauI GCSGC 4 cut(s) 43, 247, 343, 629
TfiI GAWTC 4 cut(s) 145, 178, 618, 1202
Tru1I TTAA 5 cut(s) 23, 471, 632, 1011, 1154
Tru9I TTAA 5 cut(s) 23, 471, 632, 1011, 1154
TscAI CASTG 2 cut(s) 336, 363
TseFI GTSAC 3 cut(s) 428, 601, 1180
TseI GCWGC 4 cut(s) 227, 321, 324, 699
Tsp45I GTSAC 3 cut(s) 428, 601, 1180
TspDTI ATGAA 3 cut(s) 629, 899, 977
TspGWI ACGGA 1 cut(s) 1214
TspRI CASTG 2 cut(s) 336, 363
Vha464I CTTAAG 2 cut(s) 22, 1153
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 579, 973, 1176
XapI RAATTY 2 cut(s) 452, 553
XbaI TCTAGA 2 cut(s) 133, 190
XceI RCATGY 4 cut(s) 154, 757, 830, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 558
XmaJI CCTAGG 2 cut(s) 257, 335
XmiI GTMKAC 1 cut(s) 1131
Zsp2I ATGCAT 2 cut(s) 6, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.