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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 111 |
| AclWI | GGATC | 3 cut(s) | 188, 201, 216 |
| AcsI | RAATTY | 1 cut(s) | 292 |
| AfaI | GTAC | 2 cut(s) | 333, 385 |
| AgsI | TTSAA | 2 cut(s) | 292, 406 |
| Alw26I | GTCTC | 2 cut(s) | 239, 321 |
| AlwI | GGATC | 3 cut(s) | 188, 201, 216 |
| AoxI | GGCC | 2 cut(s) | 242, 355 |
| ApoI | RAATTY | 1 cut(s) | 292 |
| AspS9I | GGNCC | 1 cut(s) | 242 |
| AsuC2I | CCSGG | 1 cut(s) | 198 |
| AsuII | TTCGAA | 1 cut(s) | 275 |
| BamHI | GGATCC | 1 cut(s) | 193 |
| BbsI | GAAGAC | 1 cut(s) | 68 |
| BccI | CCATC | 3 cut(s) | 101, 370, 374 |
| BcnI | CCSGG | 1 cut(s) | 198 |
| BcoDI | GTCTC | 2 cut(s) | 239, 321 |
| Bme1390I | CCNGG | 1 cut(s) | 198 |
| BmgT120I | GGNCC | 1 cut(s) | 242 |
| BmiI | GGNNCC | 1 cut(s) | 195 |
| BmrFI | CCNGG | 1 cut(s) | 198 |
| BpiI | GAAGAC | 1 cut(s) | 68 |
| Bpu14I | TTCGAA | 1 cut(s) | 275 |
| BpuMI | CCSGG | 1 cut(s) | 198 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 272, 302 |
| BseGI | GGATG | 2 cut(s) | 381, 385 |
| BseRI | GAGGAG | 1 cut(s) | 17 |
| Bsh1285I | CGRYCG | 1 cut(s) | 115 |
| BshFI | GGCC | 2 cut(s) | 244, 357 |
| BsiEI | CGRYCG | 1 cut(s) | 115 |
| BsiSI | CCGG | 1 cut(s) | 197 |
| BsmAI | GTCTC | 2 cut(s) | 239, 321 |
| BsmBI | CGTCTC | 1 cut(s) | 321 |
| BsnI | GGCC | 2 cut(s) | 244, 357 |
| Bsp119I | TTCGAA | 1 cut(s) | 275 |
| Bsp143I | GATC | 2 cut(s) | 193, 208 |
| BspACI | CCGC | 1 cut(s) | 111 |
| BspANI | GGCC | 2 cut(s) | 244, 357 |
| BspLI | GGNNCC | 1 cut(s) | 195 |
| BspPI | GGATC | 3 cut(s) | 188, 201, 216 |
| BspT104I | TTCGAA | 1 cut(s) | 275 |
| BssMI | GATC | 2 cut(s) | 193, 208 |
| Bst4CI | ACNGT | 3 cut(s) | 169, 266, 340 |
| BstBI | TTCGAA | 1 cut(s) | 275 |
| BstC8I | GCNNGC | 2 cut(s) | 144, 348 |
| BstF5I | GGATG | 2 cut(s) | 381, 385 |
| BstKTI | GATC | 2 cut(s) | 196, 211 |
| BstMAI | GTCTC | 2 cut(s) | 239, 321 |
| BstMBI | GATC | 2 cut(s) | 193, 208 |
| BstMCI | CGRYCG | 1 cut(s) | 115 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 143 |
| BstSCI | CCNGG | 1 cut(s) | 196 |
| BstV2I | GAAGAC | 1 cut(s) | 68 |
| BstX2I | RGATCY | 2 cut(s) | 193, 208 |
| BstYI | RGATCY | 2 cut(s) | 193, 208 |
| BsuRI | GGCC | 2 cut(s) | 244, 357 |
| BtgZI | GCGATG | 1 cut(s) | 120 |
| BtsCI | GGATG | 2 cut(s) | 381, 385 |
| BtsIMutI | CAGTG | 2 cut(s) | 174, 345 |
| Cac8I | GCNNGC | 2 cut(s) | 144, 348 |
| Cfr13I | GGNCC | 1 cut(s) | 242 |
| Csp6I | GTAC | 2 cut(s) | 332, 384 |
| CviAII | CATG | 2 cut(s) | 72, 227 |
| CviJI | RGCY | 4 cut(s) | 18, 157, 244, 357 |
| CviKI_1 | RGCY | 4 cut(s) | 18, 157, 244, 357 |
| CviQI | GTAC | 2 cut(s) | 332, 384 |
| DpnI | GATC | 2 cut(s) | 195, 210 |
| DpnII | GATC | 2 cut(s) | 193, 208 |
| EcoRI | GAATTC | 1 cut(s) | 292 |
| Esp3I | CGTCTC | 1 cut(s) | 321 |
| FaeI | CATG | 2 cut(s) | 75, 230 |
| FaiI | YATR | 2 cut(s) | 73, 228 |
| FatI | CATG | 2 cut(s) | 71, 226 |
| FokI | GGATG | 2 cut(s) | 388, 392 |
| HaeIII | GGCC | 2 cut(s) | 244, 357 |
| HapII | CCGG | 1 cut(s) | 197 |
| Hin1II | CATG | 2 cut(s) | 75, 230 |
| HincII | GTYRAC | 2 cut(s) | 285, 309 |
| HindII | GTYRAC | 2 cut(s) | 285, 309 |
| HinfI | GANTC | 1 cut(s) | 247 |
| HpaII | CCGG | 1 cut(s) | 197 |
| Hpy166II | GTNNAC | 2 cut(s) | 285, 309 |
| Hpy188I | TCNGA | 1 cut(s) | 208 |
| Hpy8I | GTNNAC | 2 cut(s) | 285, 309 |
| Hpy99I | CGWCG | 1 cut(s) | 323 |
| HpyAV | CCTTC | 3 cut(s) | 271, 325, 392 |
| HpyCH4III | ACNGT | 3 cut(s) | 169, 266, 340 |
| HpyCH4IV | ACGT | 3 cut(s) | 287, 330, 386 |
| HpyCH4V | TGCA | 2 cut(s) | 217, 346 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 143 |
| HpySE526I | ACGT | 3 cut(s) | 287, 330, 386 |
| Hsp92II | CATG | 2 cut(s) | 75, 230 |
| Kzo9I | GATC | 2 cut(s) | 193, 208 |
| LpnPI | CCDG | 4 cut(s) | 156, 210, 332, 339 |
| MaeII | ACGT | 3 cut(s) | 287, 330, 386 |
| MalI | GATC | 2 cut(s) | 195, 210 |
| MboI | GATC | 2 cut(s) | 193, 208 |
| MboII | GAAGA | 3 cut(s) | 3, 68, 288 |
| MflI | RGATCY | 2 cut(s) | 193, 208 |
| MluCI | AATT | 2 cut(s) | 292, 366 |
| MlyI | GAGTC | 1 cut(s) | 241 |
| MmeI | TCCRAC | 3 cut(s) | 109, 186, 369 |
| MnlI | CCTC | 7 cut(s) | 35, 38, 118, 179, 194, 253, 385 |
| MspI | CCGG | 1 cut(s) | 197 |
| MspR9I | CCNGG | 1 cut(s) | 198 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 143 |
| NciI | CCSGG | 1 cut(s) | 198 |
| NdeII | GATC | 2 cut(s) | 193, 208 |
| NlaIII | CATG | 2 cut(s) | 75, 230 |
| NlaIV | GGNNCC | 1 cut(s) | 195 |
| NmeAIII | GCCGAG | 1 cut(s) | 234 |
| NspV | TTCGAA | 1 cut(s) | 275 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 327 |
| PfoI | TCCNGGA | 1 cut(s) | 196 |
| PleI | GAGTC | 1 cut(s) | 241 |
| PpsI | GAGTC | 1 cut(s) | 241 |
| PspN4I | GGNNCC | 1 cut(s) | 195 |
| PspPI | GGNCC | 1 cut(s) | 242 |
| PsuI | RGATCY | 2 cut(s) | 193, 208 |
| RsaI | GTAC | 2 cut(s) | 333, 385 |
| RsaNI | GTAC | 2 cut(s) | 332, 384 |
| Sau3AI | GATC | 2 cut(s) | 193, 208 |
| Sau96I | GGNCC | 1 cut(s) | 242 |
| SchI | GAGTC | 1 cut(s) | 241 |
| ScrFI | CCNGG | 1 cut(s) | 198 |
| SetI | ASST | 8 cut(s) | 27, 70, 129, 290, 317, 333, 389, 403 |
| SfuI | TTCGAA | 1 cut(s) | 275 |
| Sse9I | AATT | 2 cut(s) | 292, 366 |
| SsiI | CCGC | 1 cut(s) | 111 |
| StyD4I | CCNGG | 1 cut(s) | 196 |
| TaaI | ACNGT | 3 cut(s) | 169, 266, 340 |
| TaiI | ACGT | 3 cut(s) | 290, 333, 389 |
| TaqI | TCGA | 3 cut(s) | 21, 275, 318 |
| TasI | AATT | 2 cut(s) | 292, 366 |
| TscAI | CASTG | 2 cut(s) | 174, 345 |
| TspDTI | ATGAA | 3 cut(s) | 60, 73, 215 |
| TspRI | CASTG | 2 cut(s) | 174, 345 |
| XapI | RAATTY | 1 cut(s) | 292 |