Rmu_sc0001577.1_g000006

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001577.1
OG0001695
Physical Location & Seq
Forward (+)
42864 .. 44882
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001577.1_g000006.1.cds

Sequence Viewer

Length: 1032 bp
atgaaactcctaattgtatcttctgtcaaggcctctgacgccaaaaccaacaaatgttgtcaacttgagaaggcattttcgtggaatttgggtagaaacaaggtattaattggtggcccatggagtttcaatcgtgctttggttgctatacaaccatatgatggagtgtcgtctctgcaatctgtagagattgaggagttattttttgggttgtgcatcacgaatatgccgccggcatatgagcagaagaacactattgccaacgttgcttctgttgcgggtaaattccttgacctggatcaaaagctatttcatgcaactcgagaaattaggacatgccttagtgtgtatcataagggggatgcatgtaaggttgaagctttagcgccaacaggcatacctgctccaccatcagtggcaagaaggcttgagctcaatggtccctttttagggtttcagggaaatcagttcaaggatggaatgtttcactttcaaggcattaccactcccattcttccgcctattagaagatctctatttggaggacctgagacaagtaagaggattacaatgcatagaatctcaatcatacaagcaggggcggatgtacttggaaggctgtctaggctcaagcctagacaagattttgggttgaaaacccataggtatgtatgtatctctacctttcataccagcccaaagaaaaaaaaaattatccatcacagcagctcacaggctcaccgctcgcagcctcagtgcctcacacagtcaccctcactgactcacagcaacctcagtgcctcacacaggttttctgactttctcaaagaaatcccagattcccagaggccagatctcctctcgcccagtctgtccagatcgagagtctcgcctagatcgagagtctcgccggacgccgaatctgtttgctacggtggtaccagtggcggagtcatggagagagacaaagaaagagattccatcatttccatattagctgagccactgaggagtgaggaactgccgaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

37.93

Weight (kDa)

9.61

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 938
AccB7I CCANNNNNTGG 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 744
AciI CCGC 6 cut(s) 230, 278, 518, 602, 742, 948
AclI AACGTT 1 cut(s) 264
AclWI GGATC 1 cut(s) 306
AcsI RAATTY 2 cut(s) 85, 284
AcyI GRCGYC 2 cut(s) 39, 915
AfaI GTAC 2 cut(s) 609, 940
AfiI CCNNNNNNNGG 5 cut(s) 161, 295, 449, 450, 807
AgsI TTSAA 5 cut(s) 130, 377, 472, 494, 655
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 5 cut(s) 307, 380, 433, 729, 998
AluI AGCT 5 cut(s) 307, 380, 433, 729, 998
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 5 cut(s) 177, 545, 892, 910, 957
AlwI GGATC 1 cut(s) 306
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 3 cut(s) 30, 115, 848
ApeKI GCWGC 2 cut(s) 726, 748
ApoI RAATTY 2 cut(s) 85, 284
AseI ATTAAT 1 cut(s) 107
Asp718I GGTACC 1 cut(s) 938
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 3 cut(s) 116, 440, 545
AsuHPI GGTGA 2 cut(s) 731, 762
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 2 cut(s) 440, 545
BaeI ACNNNNGTAYC 2 cut(s) 922, 955
BanI GGYRCC 1 cut(s) 938
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 738, 760
BccI CCATC 5 cut(s) 155, 418, 470, 726, 989
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 5 cut(s) 177, 545, 892, 910, 957
BfaI CTAG 3 cut(s) 624, 636, 894
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 2 cut(s) 530, 853
BisI GCNGC 3 cut(s) 230, 727, 749
BlpI GCTNAGC 1 cut(s) 999
BlsI GCNGC 3 cut(s) 231, 728, 750
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 440, 545
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 116, 440, 545
BmiI GGNNCC 2 cut(s) 442, 940
BmrFI CCNGG 1 cut(s) 296
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 2 cut(s) 225, 352
BmuI ACTGGG 1 cut(s) 861
Bpu1102I GCTNAGC 1 cut(s) 999
BpuEI CTTGAG 3 cut(s) 86, 449, 614
BsaHI GRCGYC 2 cut(s) 39, 915
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 295, 449, 450, 807
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 867, 942
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 3 cut(s) 367, 481, 610
BseLI CCNNNNNNNGG 5 cut(s) 161, 295, 449, 450, 807
BseMII CTCAG 5 cut(s) 540, 767, 808, 990, 998
BseNI ACTGG 2 cut(s) 867, 942
BseRI GAGGAG 3 cut(s) 209, 848, 1024
BseXI GCAGC 2 cut(s) 738, 760
BshFI GGCC 3 cut(s) 32, 117, 850
BshNI GGYRCC 1 cut(s) 938
BsiHKAI GWGCWC 1 cut(s) 435
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 233, 911
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 5 cut(s) 161, 295, 449, 450, 807
BsmAI GTCTC 5 cut(s) 177, 545, 892, 910, 957
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 426
BsnI GGCC 3 cut(s) 32, 117, 850
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 5 cut(s) 298, 530, 853, 878, 896
Bsp1720I GCTNAGC 1 cut(s) 999
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 6 cut(s) 230, 278, 518, 602, 742, 948
BspANI GGCC 3 cut(s) 32, 117, 850
BspCNI CTCAG 5 cut(s) 541, 766, 807, 991, 999
BspLI GGNNCC 2 cut(s) 442, 940
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 938
BsrBI CCGCTC 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 2 cut(s) 867, 942
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 5 cut(s) 298, 530, 853, 878, 896
BssNI GRCGYC 2 cut(s) 39, 915
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 768, 935
BstACI GRCGYC 2 cut(s) 39, 915
BstC8I GCNNGC 2 cut(s) 234, 746
BstDEI CTNAG 6 cut(s) 341, 549, 753, 794, 999, 1007
BstDSI CCRYGG 1 cut(s) 119
BstENI CCTNNNNNAGG 1 cut(s) 805
BstF5I GGATG 3 cut(s) 367, 481, 610
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 5 cut(s) 301, 533, 856, 881, 899
BstMAI GTCTC 5 cut(s) 177, 545, 892, 910, 957
BstMBI GATC 5 cut(s) 298, 530, 853, 878, 896
BstMWI GCNNNNNNNGC 5 cut(s) 38, 143, 266, 275, 625
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 2 cut(s) 339, 369
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 738, 760
BstX2I RGATCY 2 cut(s) 530, 853
BstYI RGATCY 2 cut(s) 530, 853
BsuRI GGCC 3 cut(s) 32, 117, 850
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 3 cut(s) 367, 481, 610
BtsIMutI CAGTG 6 cut(s) 420, 761, 776, 802, 949, 1004
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 234, 746
CfoI GCGC 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 116, 440, 545
CseI GACGC 2 cut(s) 47, 923
Csp6I GTAC 2 cut(s) 608, 939
CviAII CATG 5 cut(s) 120, 314, 336, 366, 955
CviQI GTAC 2 cut(s) 608, 939
DdeI CTNAG 6 cut(s) 341, 549, 753, 794, 999, 1007
DpnI GATC 5 cut(s) 300, 532, 855, 880, 898
DpnII GATC 5 cut(s) 298, 530, 853, 878, 896
EciI GGCGGA 3 cut(s) 507, 617, 963
Ecl136II GAGCTC 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 440, 545
Eco53kI GAGCTC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 433
EcoNI CCTNNNNNAGG 1 cut(s) 805
EcoO109I RGGNCCY 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 119
EcoT22I ATGCAT 2 cut(s) 367, 576
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 119
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 5 cut(s) 123, 317, 339, 369, 958
FaqI GGGAC 1 cut(s) 426
FatI CATG 5 cut(s) 119, 313, 335, 365, 954
FauI CCCGC 1 cut(s) 271
FauNDI CATATG 2 cut(s) 157, 238
Fnu4HI GCNGC 3 cut(s) 230, 727, 749
FokI GGATG 3 cut(s) 374, 488, 617
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 3 cut(s) 230, 727, 749
FspBI CTAG 3 cut(s) 624, 636, 894
GlaI GCGC 1 cut(s) 387
GluI GCNGC 3 cut(s) 230, 727, 749
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 3 cut(s) 32, 117, 850
HapII CCGG 2 cut(s) 233, 911
HgaI GACGC 2 cut(s) 47, 923
HhaI GCGC 1 cut(s) 388
Hin1I GRCGYC 2 cut(s) 39, 915
Hin1II CATG 5 cut(s) 123, 317, 339, 369, 958
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HincII GTYRAC 1 cut(s) 62
HindII GTYRAC 1 cut(s) 62
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 8 cut(s) 579, 781, 839, 885, 903, 920, 951, 977
HpaII CCGG 2 cut(s) 233, 911
HphI GGTGA 2 cut(s) 731, 762
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 2 cut(s) 37, 817
Hpy188III TCNNGA 5 cut(s) 220, 323, 876, 882, 900
Hpy8I GTNNAC 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 64, 417, 609
HpyCH4III ACNGT 2 cut(s) 768, 935
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 178, 216, 317, 365, 574
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 143, 266, 275, 625
HpyF3I CTNAG 6 cut(s) 341, 549, 753, 794, 999, 1007
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 2 cut(s) 39, 915
Hsp92II CATG 5 cut(s) 123, 317, 339, 369, 958
HspAI GCGC 1 cut(s) 386
KpnI GGTACC 1 cut(s) 942
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 5 cut(s) 298, 530, 853, 878, 896
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 2 cut(s) 738, 760
LweI GCATC 2 cut(s) 225, 352
MaeI CTAG 3 cut(s) 624, 636, 894
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 768
MalI GATC 5 cut(s) 300, 532, 855, 880, 898
MbiI CCGCTC 1 cut(s) 744
MboI GATC 5 cut(s) 298, 530, 853, 878, 896
MboII GAAGA 4 cut(s) 12, 259, 506, 540
MflI RGATCY 2 cut(s) 530, 853
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 6 cut(s) 12, 85, 108, 284, 327, 711
MlyI GAGTC 4 cut(s) 775, 894, 912, 960
Mph1103I ATGCAT 2 cut(s) 367, 576
MroNI GCCGGC 1 cut(s) 232
MseI TTAA 1 cut(s) 107
MslI CAYNNNNRTG 3 cut(s) 79, 224, 666
MspI CCGG 2 cut(s) 233, 911
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 5 cut(s) 38, 143, 266, 275, 625
NaeI GCCGGC 1 cut(s) 234
NcoI CCATGG 1 cut(s) 119
NdeI CATATG 2 cut(s) 157, 238
NdeII GATC 5 cut(s) 298, 530, 853, 878, 896
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 5 cut(s) 123, 317, 339, 369, 958
NlaIV GGNNCC 2 cut(s) 442, 940
NmuCI GTSAC 1 cut(s) 768
NsiI ATGCAT 2 cut(s) 367, 576
NspI RCATGY 2 cut(s) 339, 369
PaeR7I CTCGAG 1 cut(s) 321
PceI AGGCCT 1 cut(s) 32
PcsI WCGNNNNNNNCGW 1 cut(s) 896
PdiI GCCGGC 1 cut(s) 234
PfeI GAWTC 4 cut(s) 579, 839, 920, 977
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 3 cut(s) 231, 728, 750
PleI GAGTC 4 cut(s) 775, 893, 911, 959
PpsI GAGTC 4 cut(s) 775, 893, 911, 959
PpuMI RGGWCCY 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 435
Psp1406I AACGTT 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 442, 940
PspPI GGNCC 3 cut(s) 116, 440, 545
PspPPI RGGWCCY 1 cut(s) 545
PsuI RGATCY 2 cut(s) 530, 853
RsaI GTAC 2 cut(s) 609, 940
RsaNI GTAC 2 cut(s) 608, 939
RseI CAYNNNNRTG 3 cut(s) 79, 224, 666
SacI GAGCTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 3 cut(s) 230, 727, 749
Sau3AI GATC 5 cut(s) 298, 530, 853, 878, 896
Sau96I GGNCC 3 cut(s) 116, 440, 545
SchI GAGTC 4 cut(s) 775, 894, 912, 960
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 2 cut(s) 225, 352
SfcI CTRYAG 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 321
SinI GGWCC 2 cut(s) 440, 545
SlaI CTCGAG 1 cut(s) 321
SmiMI CAYNNNNRTG 3 cut(s) 79, 224, 666
SmlI CTYRAG 4 cut(s) 65, 321, 428, 629
SmoI CTYRAG 4 cut(s) 65, 321, 428, 629
Sse9I AATT 6 cut(s) 12, 85, 108, 284, 327, 711
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 6 cut(s) 230, 278, 518, 602, 742, 948
SspMI CTAG 3 cut(s) 624, 636, 894
SstI GAGCTC 1 cut(s) 435
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 2 cut(s) 768, 935
TaiI ACGT 1 cut(s) 267
TaqI TCGA 3 cut(s) 322, 881, 899
TasI AATT 6 cut(s) 12, 85, 108, 284, 327, 711
TatI WGTACW 1 cut(s) 607
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 4 cut(s) 579, 839, 920, 977
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 6 cut(s) 420, 761, 783, 802, 949, 1011
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 2 cut(s) 726, 748
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 3 cut(s) 17, 302, 677
TspRI CASTG 6 cut(s) 420, 761, 783, 802, 949, 1011
Van91I CCANNNNNTGG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 440, 545
VspI ATTAAT 1 cut(s) 107
XagI CCTNNNNNAGG 1 cut(s) 805
XapI RAATTY 2 cut(s) 85, 284
XceI RCATGY 2 cut(s) 339, 369
XhoI CTCGAG 1 cut(s) 321
XspI CTAG 3 cut(s) 624, 636, 894
Zsp2I ATGCAT 2 cut(s) 367, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.