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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 37 |
| AdeI | CACNNNGTG | 1 cut(s) | 96 |
| AluBI | AGCT | 1 cut(s) | 40 |
| AluI | AGCT | 1 cut(s) | 40 |
| AspS9I | GGNCC | 1 cut(s) | 116 |
| AvaII | GGWCC | 1 cut(s) | 116 |
| BaeGI | GKGCMC | 1 cut(s) | 92 |
| BfaI | CTAG | 1 cut(s) | 120 |
| Bme18I | GGWCC | 1 cut(s) | 116 |
| BmgT120I | GGNCC | 1 cut(s) | 116 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 10, 40 |
| BseSI | GKGCMC | 1 cut(s) | 92 |
| Bsp1286I | GDGCHC | 1 cut(s) | 92 |
| Bst4CI | ACNGT | 1 cut(s) | 94 |
| BstSLI | GKGCMC | 1 cut(s) | 92 |
| BtsIMutI | CAGTG | 1 cut(s) | 99 |
| Cfr13I | GGNCC | 1 cut(s) | 116 |
| CviAII | CATG | 2 cut(s) | 34, 48 |
| CviJI | RGCY | 2 cut(s) | 40, 57 |
| CviKI_1 | RGCY | 2 cut(s) | 40, 57 |
| DraIII | CACNNNGTG | 1 cut(s) | 96 |
| Eco47I | GGWCC | 1 cut(s) | 116 |
| Eco57I | CTGAAG | 1 cut(s) | 37 |
| FaeI | CATG | 2 cut(s) | 37, 51 |
| FaiI | YATR | 2 cut(s) | 35, 49 |
| FatI | CATG | 2 cut(s) | 33, 47 |
| FspBI | CTAG | 1 cut(s) | 120 |
| Hin1II | CATG | 2 cut(s) | 37, 51 |
| HinfI | GANTC | 2 cut(s) | 13, 109 |
| Hpy99I | CGWCG | 2 cut(s) | 13, 65 |
| HpyAV | CCTTC | 3 cut(s) | 33, 82, 146 |
| HpyCH4III | ACNGT | 1 cut(s) | 94 |
| Hsp92II | CATG | 2 cut(s) | 37, 51 |
| LmnI | GCTCC | 1 cut(s) | 37 |
| MaeI | CTAG | 1 cut(s) | 120 |
| MboII | GAAGA | 1 cut(s) | 36 |
| MhlI | GDGCHC | 1 cut(s) | 92 |
| MlyI | GAGTC | 1 cut(s) | 22 |
| MnlI | CCTC | 3 cut(s) | 68, 97, 139 |
| NlaIII | CATG | 2 cut(s) | 37, 51 |
| PfeI | GAWTC | 1 cut(s) | 109 |
| PleI | GAGTC | 1 cut(s) | 21 |
| PpsI | GAGTC | 1 cut(s) | 21 |
| PspPI | GGNCC | 1 cut(s) | 116 |
| Sau96I | GGNCC | 1 cut(s) | 116 |
| SchI | GAGTC | 1 cut(s) | 22 |
| SduI | GDGCHC | 1 cut(s) | 92 |
| SetI | ASST | 2 cut(s) | 25, 42 |
| SgeI | CNNG | 9 cut(s) | 24, 46, 60, 71, 76, 97, 125, 132, 155 |
| SinI | GGWCC | 1 cut(s) | 116 |
| SspMI | CTAG | 1 cut(s) | 120 |
| TaaI | ACNGT | 1 cut(s) | 94 |
| TaqI | TCGA | 1 cut(s) | 11 |
| TfiI | GAWTC | 1 cut(s) | 109 |
| TscAI | CASTG | 1 cut(s) | 99 |
| TspDTI | ATGAA | 3 cut(s) | 22, 36, 64 |
| TspRI | CASTG | 1 cut(s) | 99 |
| VpaK11BI | GGWCC | 1 cut(s) | 116 |
| XspI | CTAG | 1 cut(s) | 120 |