Rmu_sc0032474.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032474.1
OG0001695
Physical Location & Seq
Reverse (-)
6710 .. 10172
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032474.1_g000002.1.cds

Sequence Viewer

Length: 1191 bp
atgacttcaagccctggacttctccagccctcgatcaaagccgtttctccggccctgggtataaaaatggccttaattgcagcgacgacggtggaggaagtcagtggttttctggacaaggaggaagcagaggtggctgaagggtgcaaaacggcgagttattcgcagaggcatgactggcagaagccagaaattctccttcgggtggcagtcgtaagaggaggattgaaaagctgtttgatgcagccgtcgatggcctccactatgagatggtgcagagtgcgagctatggtggccattgtaaatgatgaggaggaggatgaagagagtgcaataaaggaggagaagaagaagaggacaagagtggagaatgaggagttaaggacagctgatgtgaagttgcgaaccattaggggcacattaggaatttacccattcatgtgggcccacttgctggatttgtcagagccagctcatcatttaacggtttgtggagcggcgccgctttcgggtccgtcgctacctataccttctccattcatggagatttctttaggcttcggcctcctcttgtttatccttgctgtttccaaaagcaaagggttaggttcgtatcttctatttactggtttgttgcttgtcaacgatggcggtcatattagcgaggttggagtagagttgaaggcgagagaagggcttgttaggacggcggttgttggtggtggagggtgggatccgggagggtcggatccaggggcagatgtacttggaaggctatctaggctcaagcctagacaagattttgggttgaaaacccataggtatatatgtatctctacctttcataccagcctaaagaaaaaaaaaattatccatcacagcagctcacaggctcaactctcgcagcctcaatgcttcacacagtcacactcactgactcacagcaacctcagtgcctcacacaggttttctgactttctcaaaaaaaaattcctagattcccagaggccagatctcctctcgcccagtctgcccagatcgagagtcttgcctagatcgagaggctcgccggacgccgaatctgtttgctacggtgctaccagtggcggagtcgcggagagagacagagacagagattccatcatttccatgctagttgagccgctgaggagtgaggaactgccgaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

43.26

Weight (kDa)

8.75

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 454
AccBSI CCGCTC 1 cut(s) 497
AccII CGCG 1 cut(s) 1115
AciI CCGC 7 cut(s) 497, 503, 651, 710, 1107, 1115, 1163
AclWI GGATC 4 cut(s) 728, 741, 743, 756
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 3 cut(s) 192, 426, 989
AcuI CTGAAG 1 cut(s) 159
AcyI GRCGYC 2 cut(s) 500, 1074
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 5 cut(s) 56, 205, 454, 509, 963
AgsI TTSAA 4 cut(s) 9, 229, 682, 811
AjnI CCWGG 3 cut(s) 13, 54, 751
AluBI AGCT 5 cut(s) 234, 287, 389, 473, 885
AluI AGCT 5 cut(s) 234, 287, 389, 473, 885
Alw26I GTCTC 2 cut(s) 1116, 1122
AlwI GGATC 4 cut(s) 728, 741, 743, 756
AoxI GGCC 7 cut(s) 51, 69, 255, 294, 444, 562, 1007
ApaI GGGCCC 1 cut(s) 448
ApeKI GCWGC 4 cut(s) 80, 244, 882, 904
ApoI RAATTY 3 cut(s) 192, 426, 989
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 4 cut(s) 52, 444, 445, 512
AsuC2I CCSGG 1 cut(s) 738
AvaII GGWCC 1 cut(s) 512
BaeGI GKGCMC 2 cut(s) 419, 448
BalI TGGCCA 1 cut(s) 296
BamHI GGATCC 2 cut(s) 733, 748
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 448
BbvCI CCTCAGC 1 cut(s) 1166
BbvI GCAGC 4 cut(s) 92, 256, 894, 916
BccI CCATC 5 cut(s) 247, 264, 641, 882, 1148
BceAI ACGGC 4 cut(s) 26, 168, 232, 723
BcgI CGANNNNNNTGC 2 cut(s) 1030, 1064
BciT130I CCWGG 3 cut(s) 15, 56, 753
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 2 cut(s) 1116, 1122
BfaI CTAG 5 cut(s) 780, 792, 995, 1053, 1154
BfoI RGCGCY 1 cut(s) 503
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 7 cut(s) 81, 245, 498, 503, 883, 905, 1163
BlsI GCNGC 7 cut(s) 82, 246, 499, 504, 884, 906, 1164
Bme1390I CCNGG 4 cut(s) 15, 56, 738, 753
Bme18I GGWCC 1 cut(s) 512
BmgT120I GGNCC 4 cut(s) 52, 444, 445, 512
BmiI GGNNCC 5 cut(s) 446, 501, 513, 735, 750
BmrFI CCNGG 4 cut(s) 15, 56, 738, 753
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 1 cut(s) 231
BmuI ACTGGG 1 cut(s) 1020
BpmI CTGGAG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 1166
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 738
BsaHI GRCGYC 2 cut(s) 500, 1074
BsaJI CCNNGG 4 cut(s) 13, 54, 55, 752
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 205, 454, 509, 963
Bse1I ACTGG 4 cut(s) 182, 631, 1026, 1101
BseBI CCWGG 3 cut(s) 15, 56, 753
BseDI CCNNGG 4 cut(s) 13, 54, 55, 752
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 5 cut(s) 56, 205, 454, 509, 963
BseMII CTCAG 2 cut(s) 964, 1157
BseNI ACTGG 4 cut(s) 182, 631, 1026, 1101
BseRI GAGGAG 8 cut(s) 234, 326, 329, 356, 389, 557, 1007, 1183
BseSI GKGCMC 2 cut(s) 419, 448
BseXI GCAGC 4 cut(s) 92, 256, 894, 916
BsgI GTGCAG 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 1115
BshFI GGCC 7 cut(s) 53, 71, 257, 296, 446, 564, 1009
BshNI GGYRCC 1 cut(s) 499
BsiSI CCGG 3 cut(s) 50, 737, 1070
BslI CCNNNNNNNGG 5 cut(s) 56, 205, 454, 509, 963
BsmAI GTCTC 2 cut(s) 1116, 1122
BsnI GGCC 7 cut(s) 53, 71, 257, 296, 446, 564, 1009
Bsp120I GGGCCC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 419, 448
Bsp143I GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
BspACI CCGC 7 cut(s) 497, 503, 651, 710, 1107, 1115, 1163
BspANI GGCC 7 cut(s) 53, 71, 257, 296, 446, 564, 1009
BspCNI CTCAG 2 cut(s) 963, 1158
BspFNI CGCG 1 cut(s) 1115
BspLI GGNNCC 5 cut(s) 446, 501, 513, 735, 750
BspPI GGATC 4 cut(s) 728, 741, 743, 756
BspT107I GGYRCC 1 cut(s) 499
BsrBI CCGCTC 1 cut(s) 497
BsrI ACTGG 4 cut(s) 182, 631, 1026, 1101
BssECI CCNNGG 4 cut(s) 13, 54, 55, 752
BssMI GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
BssNI GRCGYC 2 cut(s) 500, 1074
Bst2UI CCWGG 3 cut(s) 15, 56, 753
Bst4CI ACNGT 4 cut(s) 91, 487, 924, 1094
Bst6I CTCTTC 2 cut(s) 318, 347
BstACI GRCGYC 2 cut(s) 500, 1074
BstC8I GCNNGC 3 cut(s) 285, 471, 1067
BstDEI CTNAG 2 cut(s) 950, 1166
BstENI CCTNNNNNAGG 1 cut(s) 961
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 1115
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 6 cut(s) 36, 736, 751, 1015, 1040, 1058
BstMAI GTCTC 2 cut(s) 1116, 1122
BstMBI GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
BstMWI GCNNNNNNNGC 7 cut(s) 77, 134, 178, 293, 781, 1030, 1159
BstNI CCWGG 3 cut(s) 15, 56, 753
BstSCI CCNGG 4 cut(s) 13, 54, 736, 751
BstSLI GKGCMC 2 cut(s) 419, 448
BstUI CGCG 1 cut(s) 1115
BstV1I GCAGC 4 cut(s) 92, 256, 894, 916
BstX2I RGATCY 3 cut(s) 733, 748, 1012
BstXI CCANNNNNNTGG 1 cut(s) 441
BstYI RGATCY 3 cut(s) 733, 748, 1012
BsuRI GGCC 7 cut(s) 53, 71, 257, 296, 446, 564, 1009
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 4 cut(s) 109, 932, 958, 1108
Cac8I GCNNGC 3 cut(s) 285, 471, 1067
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 4 cut(s) 52, 444, 445, 512
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 1 cut(s) 764
CviAII CATG 4 cut(s) 173, 439, 541, 1150
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 2 cut(s) 950, 1166
DinI GGCGCC 1 cut(s) 501
DpnI GATC 6 cut(s) 35, 735, 750, 1014, 1039, 1057
DpnII GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 318, 347
EarI CTCTTC 2 cut(s) 318, 347
EciI GGCGGA 1 cut(s) 1122
Eco24I GRGCYC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 961
EcoRII CCWGG 3 cut(s) 13, 54, 751
EcoT38I GRGCYC 1 cut(s) 448
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
FaeI CATG 4 cut(s) 176, 442, 544, 1153
FatI CATG 4 cut(s) 172, 438, 540, 1149
Fnu4HI GCNGC 7 cut(s) 81, 245, 498, 503, 883, 905, 1163
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 7 cut(s) 81, 245, 498, 503, 883, 905, 1163
FspBI CTAG 5 cut(s) 780, 792, 995, 1053, 1154
GlaI GCGC 1 cut(s) 501
GluI GCNGC 7 cut(s) 81, 245, 498, 503, 883, 905, 1163
GsuI CTGGAG 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 7 cut(s) 53, 71, 257, 296, 446, 564, 1009
HapII CCGG 3 cut(s) 50, 737, 1070
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 2 cut(s) 500, 1074
Hin1II CATG 4 cut(s) 176, 442, 544, 1153
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HinfI GANTC 6 cut(s) 937, 998, 1044, 1079, 1110, 1136
HpaII CCGG 3 cut(s) 50, 737, 1070
Hpy166II GTNNAC 1 cut(s) 643
Hpy188I TCNGA 3 cut(s) 466, 748, 973
Hpy188III TCNNGA 3 cut(s) 113, 1041, 1059
Hpy8I GTNNAC 1 cut(s) 643
Hpy99I CGWCG 4 cut(s) 88, 91, 253, 520
HpyAV CCTTC 6 cut(s) 134, 209, 540, 676, 686, 765
HpyCH4III ACNGT 4 cut(s) 91, 487, 924, 1094
HpyCH4V TGCA 5 cut(s) 80, 147, 244, 276, 332
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 134, 178, 293, 781, 1030, 1159
HpyF3I CTNAG 2 cut(s) 950, 1166
Hsp92I GRCGYC 2 cut(s) 500, 1074
Hsp92II CATG 4 cut(s) 176, 442, 544, 1153
HspAI GCGC 1 cut(s) 500
KasI GGCGCC 1 cut(s) 499
Kzo9I GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 4 cut(s) 92, 256, 894, 916
LweI GCATC 1 cut(s) 231
MaeI CTAG 5 cut(s) 780, 792, 995, 1053, 1154
MaeIII GTNAC 1 cut(s) 924
MalI GATC 6 cut(s) 35, 735, 750, 1014, 1039, 1057
MbiI CCGCTC 1 cut(s) 497
MboI GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
MboII GAAGA 5 cut(s) 335, 358, 361, 364, 608
MflI RGATCY 3 cut(s) 733, 748, 1012
MhlI GDGCHC 2 cut(s) 419, 448
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 5 cut(s) 75, 192, 426, 867, 989
MluNI TGGCCA 1 cut(s) 296
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 3 cut(s) 931, 1053, 1119
MmeI TCCRAC 2 cut(s) 649, 726
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 3 cut(s) 74, 380, 482
MslI CAYNNNNRTG 2 cut(s) 439, 1148
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 2 cut(s) 389, 1165
MspI CCGG 3 cut(s) 50, 737, 1070
MspR9I CCNGG 4 cut(s) 15, 56, 738, 753
MvaI CCWGG 3 cut(s) 15, 56, 753
MvnI CGCG 1 cut(s) 1115
MwoI GCNNNNNNNGC 7 cut(s) 77, 134, 178, 293, 781, 1030, 1159
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 1 cut(s) 738
NdeII GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
NlaIII CATG 4 cut(s) 176, 442, 544, 1153
NlaIV GGNNCC 5 cut(s) 446, 501, 513, 735, 750
NmuCI GTSAC 1 cut(s) 924
PasI CCCWGGG 1 cut(s) 55
PfeI GAWTC 3 cut(s) 998, 1079, 1136
PflMI CCANNNNNTGG 1 cut(s) 454
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 7 cut(s) 82, 246, 499, 504, 884, 906, 1164
PleI GAGTC 3 cut(s) 931, 1052, 1118
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 3 cut(s) 931, 1052, 1118
Psp6I CCWGG 3 cut(s) 13, 54, 751
PspGI CCWGG 3 cut(s) 13, 54, 751
PspN4I GGNNCC 5 cut(s) 446, 501, 513, 735, 750
PspOMI GGGCCC 1 cut(s) 444
PspPI GGNCC 4 cut(s) 52, 444, 445, 512
PsuI RGATCY 3 cut(s) 733, 748, 1012
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 2 cut(s) 439, 1148
SaqAI TTAA 3 cut(s) 74, 380, 482
SatI GCNGC 7 cut(s) 81, 245, 498, 503, 883, 905, 1163
Sau3AI GATC 6 cut(s) 33, 733, 748, 1012, 1037, 1055
Sau96I GGNCC 4 cut(s) 52, 444, 445, 512
SchI GAGTC 3 cut(s) 931, 1053, 1119
ScrFI CCNGG 4 cut(s) 15, 56, 738, 753
SduI GDGCHC 2 cut(s) 419, 448
SfaNI GCATC 1 cut(s) 231
SfoI GGCGCC 1 cut(s) 501
SinI GGWCC 1 cut(s) 512
SmiMI CAYNNNNRTG 2 cut(s) 439, 1148
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 5 cut(s) 75, 192, 426, 867, 989
SsiI CCGC 7 cut(s) 497, 503, 651, 710, 1107, 1115, 1163
SspDI GGCGCC 1 cut(s) 499
SspMI CTAG 5 cut(s) 780, 792, 995, 1053, 1154
StyD4I CCNGG 4 cut(s) 13, 54, 736, 751
TaaI ACNGT 4 cut(s) 91, 487, 924, 1094
TaqI TCGA 4 cut(s) 32, 251, 1040, 1058
TasI AATT 5 cut(s) 75, 192, 426, 867, 989
TatI WGTACW 1 cut(s) 763
TauI GCSGC 3 cut(s) 500, 505, 1165
TfiI GAWTC 3 cut(s) 998, 1079, 1136
Tru1I TTAA 3 cut(s) 74, 380, 482
Tru9I TTAA 3 cut(s) 74, 380, 482
TscAI CASTG 4 cut(s) 109, 939, 958, 1108
TseFI GTSAC 1 cut(s) 924
TseI GCWGC 4 cut(s) 80, 244, 882, 904
Tsp45I GTSAC 1 cut(s) 924
TspDTI ATGAA 4 cut(s) 336, 427, 529, 833
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 4 cut(s) 109, 939, 958, 1108
Van91I CCANNNNNTGG 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 512
XagI CCTNNNNNAGG 1 cut(s) 961
XapI RAATTY 3 cut(s) 192, 426, 989
XspI CTAG 5 cut(s) 780, 792, 995, 1053, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.