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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 86 |
| AciI | CCGC | 1 cut(s) | 297 |
| AclWI | GGATC | 4 cut(s) | 109, 194, 207, 253 |
| AfaI | GTAC | 1 cut(s) | 281 |
| AleI | CACNNNNGTG | 1 cut(s) | 240 |
| Alw21I | GWGCWC | 1 cut(s) | 237 |
| Alw44I | GTGCAC | 1 cut(s) | 233 |
| AlwI | GGATC | 4 cut(s) | 109, 194, 207, 253 |
| ApaLI | GTGCAC | 1 cut(s) | 233 |
| AspLEI | GCGC | 1 cut(s) | 147 |
| BaeGI | GKGCMC | 1 cut(s) | 237 |
| BamHI | GGATCC | 1 cut(s) | 199 |
| BanI | GGYRCC | 1 cut(s) | 86 |
| Bbv12I | GWGCWC | 1 cut(s) | 237 |
| BccI | CCATC | 3 cut(s) | 103, 174, 283 |
| BfaI | CTAG | 1 cut(s) | 171 |
| BmiI | GGNNCC | 2 cut(s) | 88, 201 |
| BmrI | ACTGGG | 1 cut(s) | 185 |
| BmuI | ACTGGG | 1 cut(s) | 185 |
| Bpu10I | CCTNAGC | 1 cut(s) | 222 |
| BsaJI | CCNNGG | 1 cut(s) | 257 |
| Bse1I | ACTGG | 2 cut(s) | 180, 218 |
| BseDI | CCNNGG | 1 cut(s) | 257 |
| BseGI | GGATG | 1 cut(s) | 185 |
| BseNI | ACTGG | 2 cut(s) | 180, 218 |
| BseRI | GAGGAG | 1 cut(s) | 243 |
| BseSI | GKGCMC | 1 cut(s) | 237 |
| BshNI | GGYRCC | 1 cut(s) | 86 |
| BsiHKAI | GWGCWC | 1 cut(s) | 237 |
| BslFI | GGGAC | 1 cut(s) | 224 |
| BsmFI | GGGAC | 1 cut(s) | 224 |
| Bsp1286I | GDGCHC | 1 cut(s) | 237 |
| Bsp1407I | TGTACA | 1 cut(s) | 279 |
| Bsp143I | GATC | 4 cut(s) | 101, 199, 204, 245 |
| BspACI | CCGC | 1 cut(s) | 297 |
| BspLI | GGNNCC | 2 cut(s) | 88, 201 |
| BspPI | GGATC | 4 cut(s) | 109, 194, 207, 253 |
| BspT107I | GGYRCC | 1 cut(s) | 86 |
| BsrGI | TGTACA | 1 cut(s) | 279 |
| BsrI | ACTGG | 2 cut(s) | 180, 218 |
| BssECI | CCNNGG | 1 cut(s) | 257 |
| BssMI | GATC | 4 cut(s) | 101, 199, 204, 245 |
| BssT1I | CCWWGG | 1 cut(s) | 257 |
| Bst4CI | ACNGT | 1 cut(s) | 132 |
| BstAUI | TGTACA | 1 cut(s) | 279 |
| BstC8I | GCNNGC | 1 cut(s) | 295 |
| BstDEI | CTNAG | 1 cut(s) | 222 |
| BstF5I | GGATG | 1 cut(s) | 185 |
| BstHHI | GCGC | 1 cut(s) | 147 |
| BstKTI | GATC | 4 cut(s) | 104, 202, 207, 248 |
| BstMBI | GATC | 4 cut(s) | 101, 199, 204, 245 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 161, 303 |
| BstSLI | GKGCMC | 1 cut(s) | 237 |
| BstX2I | RGATCY | 2 cut(s) | 199, 245 |
| BstYI | RGATCY | 2 cut(s) | 199, 245 |
| BtgZI | GCGATG | 1 cut(s) | 122 |
| BtsCI | GGATG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 1 cut(s) | 295 |
| CfoI | GCGC | 1 cut(s) | 147 |
| Csp6I | GTAC | 1 cut(s) | 280 |
| CviAII | CATG | 1 cut(s) | 10 |
| CviJI | RGCY | 2 cut(s) | 164, 306 |
| CviKI_1 | RGCY | 2 cut(s) | 164, 306 |
| CviQI | GTAC | 1 cut(s) | 280 |
| DdeI | CTNAG | 1 cut(s) | 222 |
| DpnI | GATC | 4 cut(s) | 103, 201, 206, 247 |
| DpnII | GATC | 4 cut(s) | 101, 199, 204, 245 |
| Eco130I | CCWWGG | 1 cut(s) | 257 |
| EcoT14I | CCWWGG | 1 cut(s) | 257 |
| EcoT22I | ATGCAT | 1 cut(s) | 274 |
| ErhI | CCWWGG | 1 cut(s) | 257 |
| FaeI | CATG | 1 cut(s) | 13 |
| FaiI | YATR | 2 cut(s) | 11, 219 |
| FaqI | GGGAC | 1 cut(s) | 224 |
| FatI | CATG | 1 cut(s) | 9 |
| FokI | GGATG | 1 cut(s) | 192 |
| FspBI | CTAG | 1 cut(s) | 171 |
| GlaI | GCGC | 1 cut(s) | 146 |
| HhaI | GCGC | 1 cut(s) | 147 |
| Hin1II | CATG | 1 cut(s) | 13 |
| Hin6I | GCGC | 1 cut(s) | 145 |
| HinP1I | GCGC | 1 cut(s) | 145 |
| Hpy166II | GTNNAC | 3 cut(s) | 235, 280, 282 |
| Hpy188I | TCNGA | 2 cut(s) | 204, 311 |
| Hpy8I | GTNNAC | 3 cut(s) | 235, 280, 282 |
| Hpy99I | CGWCG | 1 cut(s) | 289 |
| HpyAV | CCTTC | 1 cut(s) | 190 |
| HpyCH4III | ACNGT | 1 cut(s) | 132 |
| HpyCH4V | TGCA | 3 cut(s) | 128, 235, 272 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 161, 303 |
| HpyF3I | CTNAG | 1 cut(s) | 222 |
| Hsp92II | CATG | 1 cut(s) | 13 |
| HspAI | GCGC | 1 cut(s) | 145 |
| Kzo9I | GATC | 4 cut(s) | 101, 199, 204, 245 |
| LpnPI | CCDG | 7 cut(s) | 69, 120, 133, 161, 193, 199, 279 |
| MaeI | CTAG | 1 cut(s) | 171 |
| MalI | GATC | 4 cut(s) | 103, 201, 206, 247 |
| MboI | GATC | 4 cut(s) | 101, 199, 204, 245 |
| MboII | GAAGA | 1 cut(s) | 11 |
| MflI | RGATCY | 2 cut(s) | 199, 245 |
| MhlI | GDGCHC | 1 cut(s) | 237 |
| MmeI | TCCRAC | 1 cut(s) | 79 |
| MnlI | CCTC | 2 cut(s) | 50, 264 |
| Mph1103I | ATGCAT | 1 cut(s) | 274 |
| MseI | TTAA | 1 cut(s) | 267 |
| MslI | CAYNNNNRTG | 2 cut(s) | 240, 287 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 161, 303 |
| NdeII | GATC | 4 cut(s) | 101, 199, 204, 245 |
| NlaIII | CATG | 1 cut(s) | 13 |
| NlaIV | GGNNCC | 2 cut(s) | 88, 201 |
| NmeAIII | GCCGAG | 1 cut(s) | 115 |
| NsiI | ATGCAT | 1 cut(s) | 274 |
| OliI | CACNNNNGTG | 1 cut(s) | 240 |
| PspN4I | GGNNCC | 2 cut(s) | 88, 201 |
| PsuI | RGATCY | 2 cut(s) | 199, 245 |
| RsaI | GTAC | 1 cut(s) | 281 |
| RsaNI | GTAC | 1 cut(s) | 280 |
| RseI | CAYNNNNRTG | 2 cut(s) | 240, 287 |
| SaqAI | TTAA | 1 cut(s) | 267 |
| Sau3AI | GATC | 4 cut(s) | 101, 199, 204, 245 |
| SduI | GDGCHC | 1 cut(s) | 237 |
| SetI | ASST | 2 cut(s) | 139, 228 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 240, 287 |
| SsiI | CCGC | 1 cut(s) | 297 |
| SspMI | CTAG | 1 cut(s) | 171 |
| StyI | CCWWGG | 1 cut(s) | 257 |
| TaaI | ACNGT | 1 cut(s) | 132 |
| TatI | WGTACW | 1 cut(s) | 279 |
| Tru1I | TTAA | 1 cut(s) | 267 |
| Tru9I | TTAA | 1 cut(s) | 267 |
| VneI | GTGCAC | 1 cut(s) | 233 |
| XspI | CTAG | 1 cut(s) | 171 |
| Zsp2I | ATGCAT | 1 cut(s) | 274 |