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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 310 |
| AciI | CCGC | 2 cut(s) | 24, 142 |
| AclI | AACGTT | 1 cut(s) | 341 |
| AcoI | YGGCCR | 2 cut(s) | 55, 153 |
| AcsI | RAATTY | 2 cut(s) | 282, 364 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 297, 310 |
| AgsI | TTSAA | 2 cut(s) | 88, 352 |
| AluBI | AGCT | 2 cut(s) | 93, 329 |
| AluI | AGCT | 2 cut(s) | 93, 329 |
| Alw26I | GTCTC | 1 cut(s) | 121 |
| Ama87I | CYCGRG | 1 cut(s) | 65 |
| AoxI | GGCC | 3 cut(s) | 55, 153, 300 |
| ApaI | GGGCCC | 1 cut(s) | 304 |
| ApeKI | GCWGC | 1 cut(s) | 103 |
| ApoI | RAATTY | 2 cut(s) | 282, 364 |
| AspS9I | GGNCC | 2 cut(s) | 300, 301 |
| AvaI | CYCGRG | 1 cut(s) | 65 |
| BaeGI | GKGCMC | 2 cut(s) | 275, 304 |
| BalI | TGGCCA | 1 cut(s) | 155 |
| BanII | GRGCYC | 1 cut(s) | 304 |
| BbvI | GCAGC | 1 cut(s) | 115 |
| BccI | CCATC | 2 cut(s) | 106, 123 |
| BceAI | ACGGC | 2 cut(s) | 91, 258 |
| BcoDI | GTCTC | 1 cut(s) | 121 |
| BisI | GCNGC | 1 cut(s) | 104 |
| BlsI | GCNGC | 1 cut(s) | 105 |
| BmeT110I | CYCGRG | 1 cut(s) | 65 |
| BmgT120I | GGNCC | 2 cut(s) | 300, 301 |
| BmiI | GGNNCC | 1 cut(s) | 302 |
| BmsI | GCATC | 3 cut(s) | 11, 90, 238 |
| BsaI | GGTCTC | 1 cut(s) | 121 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 297, 310 |
| BseGI | GGATG | 2 cut(s) | 26, 181 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 297, 310 |
| BseRI | GAGGAG | 3 cut(s) | 45, 185, 245 |
| BseSI | GKGCMC | 2 cut(s) | 275, 304 |
| BseXI | GCAGC | 1 cut(s) | 115 |
| BseYI | CCCAGC | 1 cut(s) | 310 |
| BsgI | GTGCAG | 1 cut(s) | 154 |
| BshFI | GGCC | 3 cut(s) | 57, 155, 302 |
| BsiHKCI | CYCGRG | 1 cut(s) | 65 |
| BsiSI | CCGG | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 297, 310 |
| BsmAI | GTCTC | 1 cut(s) | 121 |
| BsnI | GGCC | 3 cut(s) | 57, 155, 302 |
| Bso31I | GGTCTC | 1 cut(s) | 121 |
| BsoBI | CYCGRG | 1 cut(s) | 65 |
| Bsp120I | GGGCCC | 1 cut(s) | 300 |
| Bsp1286I | GDGCHC | 2 cut(s) | 275, 304 |
| BspACI | CCGC | 2 cut(s) | 24, 142 |
| BspANI | GGCC | 3 cut(s) | 57, 155, 302 |
| BspLI | GGNNCC | 1 cut(s) | 302 |
| BspTNI | GGTCTC | 1 cut(s) | 121 |
| Bst6I | CTCTTC | 2 cut(s) | 174, 203 |
| BstC8I | GCNNGC | 3 cut(s) | 144, 327, 359 |
| BstF5I | GGATG | 2 cut(s) | 26, 181 |
| BstMAI | GTCTC | 1 cut(s) | 121 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 152 |
| BstSLI | GKGCMC | 2 cut(s) | 275, 304 |
| BstV1I | GCAGC | 1 cut(s) | 115 |
| BsuRI | GGCC | 3 cut(s) | 57, 155, 302 |
| BtgZI | GCGATG | 1 cut(s) | 203 |
| BtsCI | GGATG | 2 cut(s) | 26, 181 |
| Cac8I | GCNNGC | 3 cut(s) | 144, 327, 359 |
| Cfr13I | GGNCC | 2 cut(s) | 300, 301 |
| CviAII | CATG | 2 cut(s) | 295, 374 |
| EaeI | YGGCCR | 2 cut(s) | 55, 153 |
| Eam1104I | CTCTTC | 2 cut(s) | 174, 203 |
| EarI | CTCTTC | 2 cut(s) | 174, 203 |
| Eco24I | GRGCYC | 1 cut(s) | 304 |
| Eco31I | GGTCTC | 1 cut(s) | 121 |
| Eco88I | CYCGRG | 1 cut(s) | 65 |
| EcoT38I | GRGCYC | 1 cut(s) | 304 |
| FaeI | CATG | 2 cut(s) | 298, 377 |
| FaiI | YATR | 4 cut(s) | 41, 149, 296, 375 |
| FatI | CATG | 2 cut(s) | 294, 373 |
| FauI | CCCGC | 1 cut(s) | 135 |
| Fnu4HI | GCNGC | 1 cut(s) | 104 |
| FokI | GGATG | 2 cut(s) | 33, 188 |
| FriOI | GRGCYC | 1 cut(s) | 304 |
| Fsp4HI | GCNGC | 1 cut(s) | 104 |
| GluI | GCNGC | 1 cut(s) | 104 |
| GsaI | CCCAGC | 1 cut(s) | 314 |
| HaeIII | GGCC | 3 cut(s) | 57, 155, 302 |
| HapII | CCGG | 1 cut(s) | 58 |
| Hin1II | CATG | 2 cut(s) | 298, 377 |
| HinfI | GANTC | 1 cut(s) | 61 |
| HpaII | CCGG | 1 cut(s) | 58 |
| Hpy188I | TCNGA | 1 cut(s) | 322 |
| Hpy99I | CGWCG | 1 cut(s) | 112 |
| HpyAV | CCTTC | 3 cut(s) | 71, 187, 301 |
| HpyCH4IV | ACGT | 1 cut(s) | 341 |
| HpyCH4V | TGCA | 2 cut(s) | 103, 135 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 152 |
| HpySE526I | ACGT | 1 cut(s) | 341 |
| Hsp92II | CATG | 2 cut(s) | 298, 377 |
| LpnPI | CCDG | 4 cut(s) | 71, 296, 339, 343 |
| Lsp1109I | GCAGC | 1 cut(s) | 115 |
| LweI | GCATC | 3 cut(s) | 11, 90, 238 |
| MaeII | ACGT | 1 cut(s) | 341 |
| MboII | GAAGA | 5 cut(s) | 191, 211, 214, 217, 220 |
| MhlI | GDGCHC | 2 cut(s) | 275, 304 |
| MlsI | TGGCCA | 1 cut(s) | 155 |
| MluCI | AATT | 3 cut(s) | 83, 282, 364 |
| MluNI | TGGCCA | 1 cut(s) | 155 |
| MnlI | CCTC | 7 cut(s) | 23, 26, 38, 163, 166, 204, 223 |
| Mox20I | TGGCCA | 1 cut(s) | 155 |
| MscI | TGGCCA | 1 cut(s) | 155 |
| MseI | TTAA | 2 cut(s) | 236, 338 |
| Msp20I | TGGCCA | 1 cut(s) | 155 |
| MspI | CCGG | 1 cut(s) | 58 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 152 |
| NlaIII | CATG | 2 cut(s) | 298, 377 |
| NlaIV | GGNNCC | 1 cut(s) | 302 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 72, 248 |
| PfeI | GAWTC | 1 cut(s) | 61 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 310 |
| PkrI | GCNGC | 1 cut(s) | 105 |
| Psp1406I | AACGTT | 1 cut(s) | 341 |
| PspFI | CCCAGC | 1 cut(s) | 310 |
| PspN4I | GGNNCC | 1 cut(s) | 302 |
| PspOMI | GGGCCC | 1 cut(s) | 300 |
| PspPI | GGNCC | 2 cut(s) | 300, 301 |
| SaqAI | TTAA | 2 cut(s) | 236, 338 |
| SatI | GCNGC | 1 cut(s) | 104 |
| Sau96I | GGNCC | 2 cut(s) | 300, 301 |
| SduI | GDGCHC | 2 cut(s) | 275, 304 |
| SetI | ASST | 3 cut(s) | 95, 331, 344 |
| SfaNI | GCATC | 3 cut(s) | 11, 90, 238 |
| Sse9I | AATT | 3 cut(s) | 83, 282, 364 |
| SsiI | CCGC | 2 cut(s) | 24, 142 |
| TaiI | ACGT | 1 cut(s) | 344 |
| TaqI | TCGA | 1 cut(s) | 110 |
| TasI | AATT | 3 cut(s) | 83, 282, 364 |
| TfiI | GAWTC | 1 cut(s) | 61 |
| Tru1I | TTAA | 2 cut(s) | 236, 338 |
| Tru9I | TTAA | 2 cut(s) | 236, 338 |
| TseI | GCWGC | 1 cut(s) | 103 |
| TspDTI | ATGAA | 3 cut(s) | 192, 206, 283 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 310 |
| XapI | RAATTY | 2 cut(s) | 282, 364 |