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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 416 |
| AccBSI | CCGCTC | 1 cut(s) | 381 |
| AciI | CCGC | 2 cut(s) | 165, 381 |
| AclWI | GGATC | 4 cut(s) | 230, 243, 253, 258 |
| AcuI | CTGAAG | 1 cut(s) | 38 |
| AfaI | GTAC | 1 cut(s) | 385 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 416 |
| Alw26I | GTCTC | 1 cut(s) | 281 |
| AlwI | GGATC | 4 cut(s) | 230, 243, 253, 258 |
| AoxI | GGCC | 2 cut(s) | 284, 407 |
| AspS9I | GGNCC | 3 cut(s) | 13, 284, 364 |
| AsuC2I | CCSGG | 1 cut(s) | 240 |
| AsuHPI | GGTGA | 1 cut(s) | 199 |
| AvaII | GGWCC | 2 cut(s) | 13, 364 |
| BamHI | GGATCC | 1 cut(s) | 235 |
| BauI | CACGAG | 1 cut(s) | 112 |
| BccI | CCATC | 3 cut(s) | 143, 189, 299 |
| BcnI | CCSGG | 1 cut(s) | 240 |
| BcoDI | GTCTC | 1 cut(s) | 281 |
| Bme1390I | CCNGG | 1 cut(s) | 240 |
| Bme18I | GGWCC | 2 cut(s) | 13, 364 |
| BmgT120I | GGNCC | 3 cut(s) | 13, 284, 364 |
| BmiI | GGNNCC | 1 cut(s) | 237 |
| BmrFI | CCNGG | 1 cut(s) | 240 |
| BpuMI | CCSGG | 1 cut(s) | 240 |
| BsaJI | CCNNGG | 1 cut(s) | 268 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 416 |
| BseDI | CCNNGG | 1 cut(s) | 268 |
| BseGI | GGATG | 2 cut(s) | 200, 310 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BseMII | CTCAG | 1 cut(s) | 292 |
| BshFI | GGCC | 2 cut(s) | 286, 409 |
| BsiSI | CCGG | 2 cut(s) | 239, 358 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BsmAI | GTCTC | 1 cut(s) | 281 |
| BsmBI | CGTCTC | 1 cut(s) | 281 |
| BsnI | GGCC | 2 cut(s) | 286, 409 |
| Bsp143I | GATC | 4 cut(s) | 235, 245, 250, 333 |
| Bsp19I | CCATGG | 1 cut(s) | 268 |
| BspACI | CCGC | 2 cut(s) | 165, 381 |
| BspANI | GGCC | 2 cut(s) | 286, 409 |
| BspCNI | CTCAG | 1 cut(s) | 291 |
| BspLI | GGNNCC | 1 cut(s) | 237 |
| BspPI | GGATC | 4 cut(s) | 230, 243, 253, 258 |
| BsrBI | CCGCTC | 1 cut(s) | 381 |
| BssECI | CCNNGG | 1 cut(s) | 268 |
| BssMI | GATC | 4 cut(s) | 235, 245, 250, 333 |
| BssSI | CACGAG | 1 cut(s) | 112 |
| BssT1I | CCWWGG | 1 cut(s) | 268 |
| Bst2BI | CACGAG | 1 cut(s) | 112 |
| BstC8I | GCNNGC | 1 cut(s) | 400 |
| BstDEI | CTNAG | 1 cut(s) | 278 |
| BstDSI | CCRYGG | 1 cut(s) | 268 |
| BstF5I | GGATG | 2 cut(s) | 200, 310 |
| BstKTI | GATC | 4 cut(s) | 238, 248, 253, 336 |
| BstMAI | GTCTC | 1 cut(s) | 281 |
| BstMBI | GATC | 4 cut(s) | 235, 245, 250, 333 |
| BstSCI | CCNGG | 1 cut(s) | 238 |
| BstX2I | RGATCY | 2 cut(s) | 235, 250 |
| BstYI | RGATCY | 2 cut(s) | 235, 250 |
| BsuRI | GGCC | 2 cut(s) | 286, 409 |
| BtgI | CCRYGG | 1 cut(s) | 268 |
| BtsCI | GGATG | 2 cut(s) | 200, 310 |
| BtsIMutI | CAGTG | 1 cut(s) | 133 |
| Cac8I | GCNNGC | 1 cut(s) | 400 |
| Cfr13I | GGNCC | 3 cut(s) | 13, 284, 364 |
| Csp6I | GTAC | 1 cut(s) | 384 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 133, 168 |
| CviAII | CATG | 3 cut(s) | 42, 269, 342 |
| CviJI | RGCY | 8 cut(s) | 59, 65, 111, 199, 286, 361, 409, 420 |
| CviKI_1 | RGCY | 8 cut(s) | 59, 65, 111, 199, 286, 361, 409, 420 |
| CviQI | GTAC | 1 cut(s) | 384 |
| DdeI | CTNAG | 1 cut(s) | 278 |
| DpnI | GATC | 4 cut(s) | 237, 247, 252, 335 |
| DpnII | GATC | 4 cut(s) | 235, 245, 250, 333 |
| Eco130I | CCWWGG | 1 cut(s) | 268 |
| Eco47I | GGWCC | 2 cut(s) | 13, 364 |
| Eco57I | CTGAAG | 1 cut(s) | 38 |
| EcoT14I | CCWWGG | 1 cut(s) | 268 |
| ErhI | CCWWGG | 1 cut(s) | 268 |
| Esp3I | CGTCTC | 1 cut(s) | 281 |
| FaeI | CATG | 3 cut(s) | 45, 272, 345 |
| FaiI | YATR | 4 cut(s) | 28, 43, 270, 343 |
| FatI | CATG | 3 cut(s) | 41, 268, 341 |
| FauI | CCCGC | 1 cut(s) | 158 |
| FokI | GGATG | 2 cut(s) | 207, 317 |
| HaeIII | GGCC | 2 cut(s) | 286, 409 |
| HapII | CCGG | 2 cut(s) | 239, 358 |
| Hin1II | CATG | 3 cut(s) | 45, 272, 345 |
| HinfI | GANTC | 1 cut(s) | 289 |
| HpaII | CCGG | 2 cut(s) | 239, 358 |
| HphI | GGTGA | 1 cut(s) | 199 |
| Hpy166II | GTNNAC | 2 cut(s) | 77, 427 |
| Hpy188I | TCNGA | 3 cut(s) | 250, 281, 338 |
| Hpy8I | GTNNAC | 2 cut(s) | 77, 427 |
| Hpy99I | CGWCG | 4 cut(s) | 21, 334, 375, 378 |
| HpyAV | CCTTC | 3 cut(s) | 41, 202, 377 |
| HpyCH4V | TGCA | 1 cut(s) | 398 |
| HpyF3I | CTNAG | 1 cut(s) | 278 |
| Hsp92II | CATG | 3 cut(s) | 45, 272, 345 |
| Kzo9I | GATC | 4 cut(s) | 235, 245, 250, 333 |
| LmnI | GCTCC | 1 cut(s) | 378 |
| LpnPI | CCDG | 6 cut(s) | 41, 170, 252, 347, 371, 391 |
| MalI | GATC | 4 cut(s) | 237, 247, 252, 335 |
| MbiI | CCGCTC | 1 cut(s) | 381 |
| MboI | GATC | 4 cut(s) | 235, 245, 250, 333 |
| MboII | GAAGA | 3 cut(s) | 44, 60, 256 |
| MflI | RGATCY | 2 cut(s) | 235, 250 |
| MlyI | GAGTC | 1 cut(s) | 283 |
| MmeI | TCCRAC | 2 cut(s) | 151, 205 |
| MnlI | CCTC | 4 cut(s) | 185, 236, 275, 295 |
| MspI | CCGG | 2 cut(s) | 239, 358 |
| MspR9I | CCNGG | 1 cut(s) | 240 |
| NciI | CCSGG | 1 cut(s) | 240 |
| NcoI | CCATGG | 1 cut(s) | 268 |
| NdeII | GATC | 4 cut(s) | 235, 245, 250, 333 |
| NlaIII | CATG | 3 cut(s) | 45, 272, 345 |
| NlaIV | GGNNCC | 1 cut(s) | 237 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 416 |
| PfoI | TCCNGGA | 1 cut(s) | 238 |
| PleI | GAGTC | 1 cut(s) | 283 |
| PpsI | GAGTC | 1 cut(s) | 283 |
| PspN4I | GGNNCC | 1 cut(s) | 237 |
| PspPI | GGNCC | 3 cut(s) | 13, 284, 364 |
| PsuI | RGATCY | 2 cut(s) | 235, 250 |
| RsaI | GTAC | 1 cut(s) | 385 |
| RsaNI | GTAC | 1 cut(s) | 384 |
| Sau3AI | GATC | 4 cut(s) | 235, 245, 250, 333 |
| Sau96I | GGNCC | 3 cut(s) | 13, 284, 364 |
| SchI | GAGTC | 1 cut(s) | 283 |
| ScrFI | CCNGG | 1 cut(s) | 240 |
| SetI | ASST | 4 cut(s) | 15, 27, 33, 369 |
| SinI | GGWCC | 2 cut(s) | 13, 364 |
| SsiI | CCGC | 2 cut(s) | 165, 381 |
| StyD4I | CCNGG | 1 cut(s) | 238 |
| StyI | CCWWGG | 1 cut(s) | 268 |
| TaqI | TCGA | 3 cut(s) | 317, 332, 370 |
| TscAI | CASTG | 1 cut(s) | 133 |
| TspDTI | ATGAA | 2 cut(s) | 30, 340 |
| TspGWI | ACGGA | 2 cut(s) | 5, 391 |
| TspRI | CASTG | 1 cut(s) | 133 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 416 |
| VpaK11BI | GGWCC | 2 cut(s) | 13, 364 |