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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 72 |
| AciI | CCGC | 3 cut(s) | 70, 258, 314 |
| AcyI | GRCGYC | 1 cut(s) | 225 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 135 |
| AluBI | AGCT | 2 cut(s) | 57, 308 |
| AluI | AGCT | 2 cut(s) | 57, 308 |
| Alw26I | GTCTC | 3 cut(s) | 220, 267, 273 |
| AoxI | GGCC | 1 cut(s) | 176 |
| ApeKI | GCWGC | 1 cut(s) | 54 |
| AsuHPI | GGTGA | 1 cut(s) | 59 |
| BbvCI | CCTCAGC | 1 cut(s) | 317 |
| BbvI | GCAGC | 1 cut(s) | 66 |
| BccI | CCATC | 2 cut(s) | 54, 299 |
| BcoDI | GTCTC | 3 cut(s) | 220, 267, 273 |
| BglII | AGATCT | 1 cut(s) | 181 |
| BisI | GCNGC | 2 cut(s) | 55, 314 |
| BlpI | GCTNAGC | 1 cut(s) | 309 |
| BlsI | GCNGC | 2 cut(s) | 56, 315 |
| BmrI | ACTGGG | 1 cut(s) | 189 |
| BmuI | ACTGGG | 1 cut(s) | 189 |
| Bpu10I | CCTNAGC | 1 cut(s) | 317 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 309 |
| BsaHI | GRCGYC | 1 cut(s) | 225 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 135 |
| Bse1I | ACTGG | 2 cut(s) | 195, 252 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 135 |
| BseMII | CTCAG | 4 cut(s) | 95, 136, 300, 308 |
| BseNI | ACTGG | 2 cut(s) | 195, 252 |
| BseRI | GAGGAG | 2 cut(s) | 176, 334 |
| BseXI | GCAGC | 1 cut(s) | 66 |
| BshFI | GGCC | 1 cut(s) | 178 |
| BsiSI | CCGG | 1 cut(s) | 221 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 135 |
| BsmAI | GTCTC | 3 cut(s) | 220, 267, 273 |
| BsnI | GGCC | 1 cut(s) | 178 |
| Bsp143I | GATC | 2 cut(s) | 181, 206 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 309 |
| BspACI | CCGC | 3 cut(s) | 70, 258, 314 |
| BspANI | GGCC | 1 cut(s) | 178 |
| BspCNI | CTCAG | 4 cut(s) | 94, 135, 301, 309 |
| BsrBI | CCGCTC | 1 cut(s) | 72 |
| BsrI | ACTGG | 2 cut(s) | 195, 252 |
| BssMI | GATC | 2 cut(s) | 181, 206 |
| BssNI | GRCGYC | 1 cut(s) | 225 |
| Bst4CI | ACNGT | 2 cut(s) | 96, 245 |
| BstACI | GRCGYC | 1 cut(s) | 225 |
| BstDEI | CTNAG | 4 cut(s) | 81, 122, 309, 317 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 133 |
| BstKTI | GATC | 2 cut(s) | 184, 209 |
| BstMAI | GTCTC | 3 cut(s) | 220, 267, 273 |
| BstMBI | GATC | 2 cut(s) | 181, 206 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 199 |
| BstV1I | GCAGC | 1 cut(s) | 66 |
| BstX2I | RGATCY | 1 cut(s) | 181 |
| BstYI | RGATCY | 1 cut(s) | 181 |
| BsuRI | GGCC | 1 cut(s) | 178 |
| BtsIMutI | CAGTG | 4 cut(s) | 89, 104, 130, 259 |
| CseI | GACGC | 1 cut(s) | 233 |
| CviAII | CATG | 1 cut(s) | 301 |
| CviJI | RGCY | 7 cut(s) | 25, 57, 65, 79, 178, 308, 313 |
| CviKI_1 | RGCY | 7 cut(s) | 25, 57, 65, 79, 178, 308, 313 |
| DdeI | CTNAG | 4 cut(s) | 81, 122, 309, 317 |
| DpnI | GATC | 2 cut(s) | 183, 208 |
| DpnII | GATC | 2 cut(s) | 181, 206 |
| EciI | GGCGGA | 1 cut(s) | 273 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 133 |
| FaeI | CATG | 1 cut(s) | 304 |
| FaiI | YATR | 2 cut(s) | 19, 302 |
| FatI | CATG | 1 cut(s) | 300 |
| Fnu4HI | GCNGC | 2 cut(s) | 55, 314 |
| Fsp4HI | GCNGC | 2 cut(s) | 55, 314 |
| GluI | GCNGC | 2 cut(s) | 55, 314 |
| HaeIII | GGCC | 1 cut(s) | 178 |
| HapII | CCGG | 1 cut(s) | 221 |
| HgaI | GACGC | 1 cut(s) | 233 |
| Hin1I | GRCGYC | 1 cut(s) | 225 |
| Hin1II | CATG | 1 cut(s) | 304 |
| HinfI | GANTC | 5 cut(s) | 109, 167, 213, 261, 287 |
| HpaII | CCGG | 1 cut(s) | 221 |
| HphI | GGTGA | 1 cut(s) | 59 |
| Hpy188I | TCNGA | 1 cut(s) | 145 |
| Hpy188III | TCNNGA | 1 cut(s) | 210 |
| HpyCH4III | ACNGT | 2 cut(s) | 96, 245 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 199 |
| HpyF3I | CTNAG | 4 cut(s) | 81, 122, 309, 317 |
| Hsp92I | GRCGYC | 1 cut(s) | 225 |
| Hsp92II | CATG | 1 cut(s) | 304 |
| Kzo9I | GATC | 2 cut(s) | 181, 206 |
| Lsp1109I | GCAGC | 1 cut(s) | 66 |
| MaeIII | GTNAC | 1 cut(s) | 96 |
| MalI | GATC | 2 cut(s) | 183, 208 |
| MbiI | CCGCTC | 1 cut(s) | 72 |
| MboI | GATC | 2 cut(s) | 181, 206 |
| MflI | RGATCY | 1 cut(s) | 181 |
| MluCI | AATT | 1 cut(s) | 39 |
| MlyI | GAGTC | 3 cut(s) | 103, 222, 270 |
| MnlI | CCTC | 8 cut(s) | 90, 98, 131, 139, 168, 197, 312, 319 |
| MslI | CAYNNNNRTG | 1 cut(s) | 299 |
| MspA1I | CMGCKG | 1 cut(s) | 316 |
| MspI | CCGG | 1 cut(s) | 221 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 199 |
| NdeII | GATC | 2 cut(s) | 181, 206 |
| NlaIII | CATG | 1 cut(s) | 304 |
| NmuCI | GTSAC | 1 cut(s) | 96 |
| PfeI | GAWTC | 2 cut(s) | 167, 287 |
| PkrI | GCNGC | 2 cut(s) | 56, 315 |
| PleI | GAGTC | 3 cut(s) | 103, 221, 269 |
| PpsI | GAGTC | 3 cut(s) | 103, 221, 269 |
| PsuI | RGATCY | 1 cut(s) | 181 |
| RseI | CAYNNNNRTG | 1 cut(s) | 299 |
| SatI | GCNGC | 2 cut(s) | 55, 314 |
| Sau3AI | GATC | 2 cut(s) | 181, 206 |
| SchI | GAGTC | 3 cut(s) | 103, 222, 270 |
| SetI | ASST | 5 cut(s) | 15, 59, 123, 140, 310 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 299 |
| Sse9I | AATT | 1 cut(s) | 39 |
| SsiI | CCGC | 3 cut(s) | 70, 258, 314 |
| TaaI | ACNGT | 2 cut(s) | 96, 245 |
| TaqI | TCGA | 1 cut(s) | 209 |
| TasI | AATT | 1 cut(s) | 39 |
| TauI | GCSGC | 1 cut(s) | 316 |
| TfiI | GAWTC | 2 cut(s) | 167, 287 |
| TscAI | CASTG | 4 cut(s) | 89, 111, 130, 259 |
| TseFI | GTSAC | 1 cut(s) | 96 |
| TseI | GCWGC | 1 cut(s) | 54 |
| Tsp45I | GTSAC | 1 cut(s) | 96 |
| TspDTI | ATGAA | 1 cut(s) | 6 |
| TspRI | CASTG | 4 cut(s) | 89, 111, 130, 259 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 133 |