Rh2DG454800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001695
Physical Location & Seq
Forward (+)
65809210 .. 65810391
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG454800.1

Sequence Viewer

Length: 570 bp
ATGGAGATTTCTTTAGGCTTCGGCCTCCTCTTGTTTATCCTTGCTGTTTCCAAAAGCAAAGGGTTAGGTTCGTATCTTCTATTTATTGGTTTGTTGCTTGTCAACGATGGCGGTCATATTACCGAGGTTGGAGTAGAGTTGCCGGCGAGAGAAGGGCTTGTTAGGACGGCGGTTGTTGGTGGTGGAGGGTGGGATCCGGGAGGGTCGGATCTCTTGCATTTCTTCCATTGTTGCCTCAAAGGGCCGACTCATCTAGGCGAGGATGGTGGCATTTCGAGTGAGCTGTCGTCGATCAGAGTGGGTTTTCTCCAGGCGGATCACCTGACGATGCTACTACCAGATTGTGGGTTCGTGGCTTGTGCTATTCTCAGAGGAGTTGACATCGAATTCTTCTTGGCAGTTGACGACCTTCGACGACGGAGCAGTATAAACAGTGTTGCAGGCTACATGCCATCGAGCAGTTTGGTGGACGGATGGTACGTTGGAAGCGGAAGGTTTGAACGCCTTTGGACTCTAGGCAATACTTGGTGCTGGGTTTGGTGGACGGATCTGGGCGGGATCCGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.42

Weight (kDa)

5.24

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 561
AciI CCGC 5 cut(s) 111, 170, 314, 489, 555
AclWI GGATC 7 cut(s) 188, 201, 216, 324, 553, 555, 566
AcsI RAATTY 1 cut(s) 386
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 344
AgsI TTSAA 1 cut(s) 500
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
AlwI GGATC 7 cut(s) 188, 201, 216, 324, 553, 555, 566
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 2 cut(s) 22, 242
ApoI RAATTY 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 311
BamHI GGATCC 2 cut(s) 193, 558
BccI CCATC 4 cut(s) 101, 257, 460, 468
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 1 cut(s) 311
BcnI CCSGG 1 cut(s) 198
BfaI CTAG 2 cut(s) 254, 515
Bme1390I CCNGG 2 cut(s) 198, 311
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 195, 560
BmrFI CCNGG 2 cut(s) 198, 311
BmsI GCATC 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 561
Bsc4I CCNNNNNNNGG 1 cut(s) 344
Bse118I RCCGGY 1 cut(s) 142
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 2 cut(s) 268, 479
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 1 cut(s) 382
BseRI GAGGAG 2 cut(s) 17, 387
BseYI CCCAGC 1 cut(s) 531
BshFI GGCC 2 cut(s) 24, 244
BsiSI CCGG 3 cut(s) 143, 197, 562
BslI CCNNNNNNNGG 1 cut(s) 344
BsnI GGCC 2 cut(s) 24, 244
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 6 cut(s) 193, 208, 291, 316, 547, 558
BspACI CCGC 5 cut(s) 111, 170, 314, 489, 555
BspANI GGCC 2 cut(s) 24, 244
BspCNI CTCAG 1 cut(s) 381
BspEI TCCGGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 195, 560
BspPI GGATC 7 cut(s) 188, 201, 216, 324, 553, 555, 566
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 6 cut(s) 193, 208, 291, 316, 547, 558
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 144, 442
BstDEI CTNAG 1 cut(s) 368
BstF5I GGATG 2 cut(s) 268, 479
BstKTI GATC 6 cut(s) 196, 211, 294, 319, 550, 561
BstMBI GATC 6 cut(s) 193, 208, 291, 316, 547, 558
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 196, 309
BstX2I RGATCY 4 cut(s) 193, 208, 547, 558
BstYI RGATCY 4 cut(s) 193, 208, 547, 558
BsuRI GGCC 2 cut(s) 24, 244
BtsCI GGATG 2 cut(s) 268, 479
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 2 cut(s) 144, 442
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 478
CviAII CATG 1 cut(s) 448
CviJI RGCY 7 cut(s) 18, 24, 157, 244, 283, 356, 444
CviKI_1 RGCY 7 cut(s) 18, 24, 157, 244, 283, 356, 444
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 1 cut(s) 368
DpnI GATC 6 cut(s) 195, 210, 293, 318, 549, 560
DpnII GATC 6 cut(s) 193, 208, 291, 316, 547, 558
EciI GGCGGA 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 309
FaeI CATG 1 cut(s) 451
FaiI YATR 4 cut(s) 117, 428, 449, 568
FatI CATG 1 cut(s) 447
FauI CCCGC 1 cut(s) 548
FokI GGATG 2 cut(s) 275, 486
FspBI CTAG 2 cut(s) 254, 515
GsaI CCCAGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 2 cut(s) 24, 244
HapII CCGG 3 cut(s) 143, 197, 562
Hin1II CATG 1 cut(s) 451
HincII GTYRAC 3 cut(s) 103, 379, 403
HindII GTYRAC 3 cut(s) 103, 379, 403
HinfI GANTC 2 cut(s) 247, 511
HpaII CCGG 3 cut(s) 143, 197, 562
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 5 cut(s) 103, 379, 403, 469, 543
Hpy188I TCNGA 3 cut(s) 208, 296, 371
Hpy188III TCNNGA 1 cut(s) 562
Hpy8I GTNNAC 5 cut(s) 103, 379, 403, 469, 543
Hpy99I CGWCG 3 cut(s) 292, 417, 420
HpyAV CCTTC 3 cut(s) 146, 419, 486
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 2 cut(s) 217, 440
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 451
Kpn2I TCCGGA 1 cut(s) 561
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 6 cut(s) 193, 208, 291, 316, 547, 558
LmnI GCTCC 1 cut(s) 420
LpnPI CCDG 9 cut(s) 156, 210, 296, 323, 335, 351, 426, 517, 536
LweI GCATC 1 cut(s) 318
MaeI CTAG 2 cut(s) 254, 515
MaeII ACGT 1 cut(s) 480
MalI GATC 6 cut(s) 195, 210, 293, 318, 549, 560
MboI GATC 6 cut(s) 193, 208, 291, 316, 547, 558
MboII GAAGA 3 cut(s) 68, 214, 382
MflI RGATCY 4 cut(s) 193, 208, 547, 558
MluCI AATT 1 cut(s) 386
MlyI GAGTC 2 cut(s) 241, 505
MmeI TCCRAC 3 cut(s) 109, 186, 463
MnlI CCTC 8 cut(s) 35, 38, 118, 179, 194, 245, 253, 365
MroI TCCGGA 1 cut(s) 561
MroNI GCCGGC 1 cut(s) 142
MspI CCGG 3 cut(s) 143, 197, 562
MspR9I CCNGG 2 cut(s) 198, 311
MvaI CCWGG 1 cut(s) 311
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 198
NdeII GATC 6 cut(s) 193, 208, 291, 316, 547, 558
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 2 cut(s) 195, 560
NspI RCATGY 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 196
PleI GAGTC 2 cut(s) 241, 505
PpsI GAGTC 2 cut(s) 241, 505
Psp6I CCWGG 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 195, 560
PspPI GGNCC 1 cut(s) 242
PsuI RGATCY 4 cut(s) 193, 208, 547, 558
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
Sau3AI GATC 6 cut(s) 193, 208, 291, 316, 547, 558
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 241, 505
ScrFI CCNGG 2 cut(s) 198, 311
SetI ASST 7 cut(s) 70, 129, 285, 324, 411, 483, 497
SfaNI GCATC 1 cut(s) 318
Sse9I AATT 1 cut(s) 386
SsiI CCGC 5 cut(s) 111, 170, 314, 489, 555
SspMI CTAG 2 cut(s) 254, 515
StyD4I CCNGG 2 cut(s) 196, 309
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 1 cut(s) 483
TaqI TCGA 5 cut(s) 275, 290, 384, 412, 455
TasI AATT 1 cut(s) 386
TscAI CASTG 1 cut(s) 439
TspGWI ACGGA 3 cut(s) 433, 486, 560
TspRI CASTG 1 cut(s) 439
Van91I CCANNNNNTGG 1 cut(s) 344
XapI RAATTY 1 cut(s) 386
XceI RCATGY 1 cut(s) 451
XspI CTAG 2 cut(s) 254, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.