Rmu_sc0001522.1_g000016

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001522.1
OG0002038
Physical Location & Seq
Reverse (-)
66068 .. 74113
8046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001522.1_g000016.1.cds

Sequence Viewer

Length: 981 bp
atgatgtctttgccttgtgaagcactgagaatgaactctccacataaggacaggcacgaaatcgagcctttatggccggcaagcacagccagtgatcggcgggctttcttcggaggcctcgctgcttgtgctgctgatgctgctggtgcttgctgctgtggcagagatggatctttgcggcagctttctctttcccaagcgtatggcgatgaggctccgactaggacggcgttgttagatctgaggctgaaagtgctagcagtgacaatggcttccttcctccaatcttcatgggtttcaatcaatcatttgactccttcgtcaatcttggcgaactggaaacttcaattaagaatccttctaaggcaaggagcagtcgaaaccctagggtataagtacagggttaagcgacgaaatcaaggacctctctcaagtcaactaatcccagcgattacaaagtctccccggagcaaaccttcaacctcgttgaaacccggtgtccgcccgccagtcctagtctccccgcgagccgactgtcagcctcaccagatcaacccggctaccatacttccagtcccagtctcctcgcgagccgactgccagtactactgccaccgatacacccagcgaagcaaggcgcttgaagaagaagtcaggtcaagggactaccccaacgactgcaccccgcggtgctggcacgcctgcgcaatcacacgctcaaaagagactgtttgcacaccagctggttttggagccaaacagaagtttgaagatatttatgtaaagcatatatatcgtgaatgcaatatggtggctaatgcacttgctaagaaggggaatgaagttgatcatggtgttcatctcatggaagaccctgctagttatgctactcaggcttatctagatgatatctctagattcagaagggttcctaggagccaggggttctcatggccctggtcagggcaggccctaccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

326

Amino Acids

36.3

Weight (kDa)

9.36

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc16I TGCGCA 1 cut(s) 706
AccII CGCG 3 cut(s) 526, 589, 688
AciI CCGC 7 cut(s) 100, 178, 502, 506, 524, 686, 688
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 1 cut(s) 74
AfaI GTAC 2 cut(s) 398, 605
AfiI CCNNNNNNNGG 3 cut(s) 96, 962, 963
AgsI TTSAA 6 cut(s) 300, 347, 480, 490, 644, 770
AjnI CCWGG 2 cut(s) 939, 956
AluBI AGCT 2 cut(s) 184, 743
AluI AGCT 2 cut(s) 184, 743
Alw26I GTCTC 4 cut(s) 465, 523, 586, 719
AlwI GGATC 1 cut(s) 178
AoxI GGCC 4 cut(s) 74, 115, 953, 969
ApeKI GCWGC 5 cut(s) 122, 131, 140, 153, 181
AspA2I CCTAGG 2 cut(s) 385, 932
AspLEI GCGC 2 cut(s) 640, 707
AspS9I GGNCC 3 cut(s) 422, 954, 970
AsuC2I CCSGG 3 cut(s) 466, 495, 557
AsuHPI GGTGA 1 cut(s) 536
AsuNHI GCTAGC 1 cut(s) 256
AvaII GGWCC 1 cut(s) 422
AvrII CCTAGG 2 cut(s) 385, 932
BbsI GAAGAC 1 cut(s) 876
BbvI GCAGC 5 cut(s) 109, 118, 127, 140, 193
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 243
BcgI CGANNNNNNTGC 4 cut(s) 579, 613, 776, 810
BciT130I CCWGG 2 cut(s) 941, 958
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 3 cut(s) 466, 495, 557
BcoDI GTCTC 4 cut(s) 465, 523, 586, 719
BfaI CTAG 8 cut(s) 222, 257, 386, 515, 879, 902, 915, 933
BfoI RGCGCY 1 cut(s) 641
BglI GCCNNNNNGGC 1 cut(s) 73
BglII AGATCT 1 cut(s) 238
BisI GCNGC 6 cut(s) 123, 132, 141, 154, 179, 182
BlnI CCTAGG 2 cut(s) 385, 932
BlsI GCNGC 6 cut(s) 124, 133, 142, 155, 180, 183
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 5 cut(s) 466, 495, 557, 941, 958
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 3 cut(s) 422, 954, 970
BmiI GGNNCC 4 cut(s) 216, 754, 930, 938
BmrFI CCNGG 5 cut(s) 466, 495, 557, 941, 958
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 1 cut(s) 127
BmtI GCTAGC 1 cut(s) 260
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 415
BpuMI CCSGG 3 cut(s) 466, 495, 557
BsaJI CCNNGG 6 cut(s) 385, 464, 686, 932, 940, 956
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 962, 963
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 6 cut(s) 90, 341, 509, 572, 578, 601
BseBI CCWGG 2 cut(s) 941, 958
BseDI CCNNGG 6 cut(s) 385, 464, 686, 932, 940, 956
BseLI CCNNNNNNNGG 3 cut(s) 96, 962, 963
BseMII CTCAG 3 cut(s) 17, 233, 905
BseNI ACTGG 6 cut(s) 90, 341, 509, 572, 578, 601
BseRI GAGGAG 1 cut(s) 574
BseXI GCAGC 5 cut(s) 109, 118, 127, 140, 193
BseYI CCCAGC 2 cut(s) 445, 624
BsgI GTGCAG 1 cut(s) 664
Bsh1236I CGCG 3 cut(s) 526, 589, 688
BshFI GGCC 4 cut(s) 76, 117, 955, 971
BsiSI CCGG 4 cut(s) 77, 466, 495, 557
BslFI GGGAC 2 cut(s) 560, 677
BslI CCNNNNNNNGG 3 cut(s) 96, 962, 963
BsmAI GTCTC 4 cut(s) 465, 523, 586, 719
BsmFI GGGAC 2 cut(s) 560, 677
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 4 cut(s) 76, 117, 955, 971
Bsp143I GATC 5 cut(s) 94, 170, 238, 549, 847
Bsp68I TCGCGA 1 cut(s) 589
BspACI CCGC 7 cut(s) 100, 178, 502, 506, 524, 686, 688
BspANI GGCC 4 cut(s) 76, 117, 955, 971
BspCNI CTCAG 3 cut(s) 18, 234, 904
BspFNI CGCG 3 cut(s) 526, 589, 688
BspLI GGNNCC 4 cut(s) 216, 754, 930, 938
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 6 cut(s) 90, 341, 509, 572, 578, 601
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 6 cut(s) 385, 464, 686, 932, 940, 956
BssMI GATC 5 cut(s) 94, 170, 238, 549, 847
BssT1I CCWWGG 2 cut(s) 385, 932
Bst2UI CCWGG 2 cut(s) 941, 958
Bst4CI ACNGT 2 cut(s) 536, 730
BstDEI CTNAG 5 cut(s) 26, 242, 362, 828, 891
BstDSI CCRYGG 1 cut(s) 686
BstFNI CGCG 3 cut(s) 526, 589, 688
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 2 cut(s) 640, 707
BstKTI GATC 5 cut(s) 97, 173, 241, 552, 850
BstMAI GTCTC 4 cut(s) 465, 523, 586, 719
BstMBI GATC 5 cut(s) 94, 170, 238, 549, 847
BstNI CCWGG 2 cut(s) 941, 958
BstSCI CCNGG 5 cut(s) 464, 493, 555, 939, 956
BstUI CGCG 3 cut(s) 526, 589, 688
BstV1I GCAGC 5 cut(s) 109, 118, 127, 140, 193
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 2 cut(s) 170, 238
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 2 cut(s) 170, 238
BsuRI GGCC 4 cut(s) 76, 117, 955, 971
BtgI CCRYGG 1 cut(s) 686
BtgZI GCGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 3 cut(s) 23, 97, 267
BtuMI TCGCGA 1 cut(s) 589
CfoI GCGC 2 cut(s) 640, 707
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 3 cut(s) 422, 954, 970
Cfr42I CCGCGG 1 cut(s) 689
Csp6I GTAC 2 cut(s) 397, 604
CviAII CATG 4 cut(s) 291, 851, 865, 951
CviQI GTAC 2 cut(s) 397, 604
DdeI CTNAG 5 cut(s) 26, 242, 362, 828, 891
DpnI GATC 5 cut(s) 96, 172, 240, 551, 849
DpnII GATC 5 cut(s) 94, 170, 238, 549, 847
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 74
EciI GGCGGA 1 cut(s) 491
Eco130I CCWWGG 2 cut(s) 385, 932
Eco147I AGGCCT 1 cut(s) 117
Eco32I GATATC 1 cut(s) 910
Eco47I GGWCC 1 cut(s) 422
EcoO109I RGGNCCY 2 cut(s) 422, 970
EcoRII CCWGG 2 cut(s) 939, 956
EcoRV GATATC 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 385, 932
ErhI CCWWGG 2 cut(s) 385, 932
FaeI CATG 4 cut(s) 294, 854, 868, 954
FaqI GGGAC 2 cut(s) 560, 677
FatI CATG 4 cut(s) 290, 850, 864, 950
FauI CCCGC 4 cut(s) 93, 513, 531, 693
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 6 cut(s) 123, 132, 141, 154, 179, 182
Fsp4HI GCNGC 6 cut(s) 123, 132, 141, 154, 179, 182
FspBI CTAG 8 cut(s) 222, 257, 386, 515, 879, 902, 915, 933
FspI TGCGCA 1 cut(s) 706
GlaI GCGC 2 cut(s) 639, 706
GluI GCNGC 6 cut(s) 123, 132, 141, 154, 179, 182
GsaI CCCAGC 2 cut(s) 449, 628
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 4 cut(s) 76, 117, 955, 971
HapII CCGG 4 cut(s) 77, 466, 495, 557
HhaI GCGC 2 cut(s) 640, 707
Hin1II CATG 4 cut(s) 294, 854, 868, 954
Hin6I GCGC 2 cut(s) 638, 705
HinP1I GCGC 2 cut(s) 638, 705
HincII GTYRAC 1 cut(s) 437
HindII GTYRAC 1 cut(s) 437
HinfI GANTC 3 cut(s) 313, 354, 918
HpaII CCGG 4 cut(s) 77, 466, 495, 557
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 1 cut(s) 437
Hpy188I TCNGA 4 cut(s) 113, 219, 243, 923
Hpy188III TCNNGA 4 cut(s) 588, 797, 902, 915
Hpy8I GTNNAC 1 cut(s) 437
Hpy99I CGWCG 1 cut(s) 414
HpyAV CCTTC 6 cut(s) 286, 327, 368, 486, 826, 918
HpyCH4III ACNGT 2 cut(s) 536, 730
HpyCH4V TGCA 4 cut(s) 681, 735, 804, 821
HpyF3I CTNAG 5 cut(s) 26, 242, 362, 828, 891
Hsp92II CATG 4 cut(s) 294, 854, 868, 954
HspAI GCGC 2 cut(s) 638, 705
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 847
KspI CCGCGG 1 cut(s) 689
Kzo9I GATC 5 cut(s) 94, 170, 238, 549, 847
LmnI GCTCC 5 cut(s) 220, 371, 468, 752, 936
Lsp1109I GCAGC 5 cut(s) 109, 118, 127, 140, 193
LweI GCATC 1 cut(s) 127
MaeI CTAG 8 cut(s) 222, 257, 386, 515, 879, 902, 915, 933
MaeIII GTNAC 1 cut(s) 262
MalI GATC 5 cut(s) 96, 172, 240, 551, 849
MboI GATC 5 cut(s) 94, 170, 238, 549, 847
MboII GAAGA 6 cut(s) 100, 279, 656, 659, 782, 881
MflI RGATCY 2 cut(s) 170, 238
MluCI AATT 1 cut(s) 347
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 9 cut(s) 107, 128, 205, 237, 290, 435, 493, 552, 595
MroNI GCCGGC 1 cut(s) 76
MseI TTAA 2 cut(s) 350, 405
MspA1I CMGCKG 2 cut(s) 688, 743
MspI CCGG 4 cut(s) 77, 466, 495, 557
MspR9I CCNGG 5 cut(s) 466, 495, 557, 941, 958
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 2 cut(s) 941, 958
MvnI CGCG 3 cut(s) 526, 589, 688
NaeI GCCGGC 1 cut(s) 78
NciI CCSGG 3 cut(s) 466, 495, 557
NdeII GATC 5 cut(s) 94, 170, 238, 549, 847
NgoMIV GCCGGC 1 cut(s) 76
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 4 cut(s) 294, 854, 868, 954
NlaIV GGNNCC 4 cut(s) 216, 754, 930, 938
NmuCI GTSAC 1 cut(s) 262
NruI TCGCGA 1 cut(s) 589
NsbI TGCGCA 1 cut(s) 706
PceI AGGCCT 1 cut(s) 117
PctI GAATGC 1 cut(s) 806
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 354, 918
PkrI GCNGC 6 cut(s) 124, 133, 142, 155, 180, 183
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 939, 956
PspFI CCCAGC 2 cut(s) 445, 624
PspGI CCWGG 2 cut(s) 939, 956
PspN4I GGNNCC 4 cut(s) 216, 754, 930, 938
PspPI GGNCC 3 cut(s) 422, 954, 970
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 2 cut(s) 170, 238
PvuII CAGCTG 1 cut(s) 743
RruI TCGCGA 1 cut(s) 589
RsaI GTAC 2 cut(s) 398, 605
RsaNI GTAC 2 cut(s) 397, 604
SacII CCGCGG 1 cut(s) 689
SaqAI TTAA 2 cut(s) 350, 405
SatI GCNGC 6 cut(s) 123, 132, 141, 154, 179, 182
Sau3AI GATC 5 cut(s) 94, 170, 238, 549, 847
Sau96I GGNCC 3 cut(s) 422, 954, 970
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 5 cut(s) 466, 495, 557, 941, 958
SetI ASST 6 cut(s) 186, 427, 478, 485, 659, 745
SfaNI GCATC 1 cut(s) 127
Sfr303I CCGCGG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 689
SinI GGWCC 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 1 cut(s) 347
SseBI AGGCCT 1 cut(s) 117
SsiI CCGC 7 cut(s) 100, 178, 502, 506, 524, 686, 688
SspMI CTAG 8 cut(s) 222, 257, 386, 515, 879, 902, 915, 933
StuI AGGCCT 1 cut(s) 117
StyD4I CCNGG 5 cut(s) 464, 493, 555, 939, 956
StyI CCWWGG 2 cut(s) 385, 932
TaaI ACNGT 2 cut(s) 536, 730
TaqI TCGA 2 cut(s) 63, 378
TasI AATT 1 cut(s) 347
TatI WGTACW 2 cut(s) 396, 603
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 2 cut(s) 354, 918
Tru1I TTAA 2 cut(s) 350, 405
Tru9I TTAA 2 cut(s) 350, 405
TscAI CASTG 3 cut(s) 30, 97, 267
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 5 cut(s) 122, 131, 140, 153, 181
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 4 cut(s) 47, 279, 848, 855
TspRI CASTG 3 cut(s) 30, 97, 267
VpaK11BI GGWCC 1 cut(s) 422
XbaI TCTAGA 2 cut(s) 901, 914
XmaJI CCTAGG 2 cut(s) 385, 932
XspI CTAG 8 cut(s) 222, 257, 386, 515, 879, 902, 915, 933
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.