Rmu_sc0016141.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016141.1
OG0002038
Physical Location & Seq
Reverse (-)
15969 .. 18783
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016141.1_g000003.1.cds

Sequence Viewer

Length: 867 bp
atgcgacagaaagcgtttcgcaaacgaaaggacgcggccttcattaatggcatcgtttcaagcgaccggcgcgcctttctagtccccttcaatcagttacatcttcgcaggcctgatttcggggcctggggggcaatgtttgagcccaaaagcatttttggcaaggtcctttgggttaagcggcgaaatctaggacttctctcaaatcaactaatcccagcgattacaaagcctccccagagcaaaccttcaacctcgttgaaacccagtgaccgcccgccagtcctagtctcctcgcgagccgactgtcagcatcaccagatcaacccggcgaccgtgctttcagtcctagtctcctcgcgagccgactgccagtactactgccaccgacacaacaggcgaagcaagggcaaaaagtcctttctcagaaggcaagagaagaaccctgtgacgaggttggttctctcctcgtccacagcgcttgaagaagaagtcaggtcaagggactaccccaacgactgcaccccgcgacgtgtcacctccgacgcggcggaccgaatcctagacgacgacatcatccccctcgtgatcgtgaagaggaccttaataatcaaacttgacaactctaactcatcatccataaatacagctctattcgaaaatcttcgaaagctgcaagttccactggaggagcgtagctccatggtagagaaagtaatgttcaaaggcttcgaggcggcacggaaatatagttcttatgctagtgtgaagagactgcgtatgcaggtgaacattgatttgtatgtgattgatgatgatgttgatgaggacacaaatgctggtgatgattatctatactattattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

33.08

Weight (kDa)

9.79

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 775
Acc36I ACCTGC 1 cut(s) 775
AccII CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
AciI CCGC 8 cut(s) 35, 181, 274, 278, 527, 548, 551, 737
AfaI GTAC 1 cut(s) 377
AfeI AGCGCT 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 6 cut(s) 60, 91, 252, 262, 485, 724
AjiI CACGTC 1 cut(s) 533
AjnI CCWGG 1 cut(s) 125
AloI GAACNNNNNNTCC 2 cut(s) 736, 768
AluBI AGCT 3 cut(s) 650, 673, 699
AluI AGCT 3 cut(s) 650, 673, 699
Alw26I GTCTC 3 cut(s) 295, 358, 766
Aor51HI AGCGCT 1 cut(s) 480
AoxI GGCC 3 cut(s) 36, 110, 123
ApeKI GCWGC 1 cut(s) 673
AscI GGCGCGCC 1 cut(s) 70
AseI ATTAAT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 3 cut(s) 72, 74, 481
AspS9I GGNCC 4 cut(s) 123, 166, 553, 600
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 4 cut(s) 308, 529, 799, 854
AsuII TTCGAA 2 cut(s) 657, 667
AvaII GGWCC 3 cut(s) 166, 553, 600
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 1 cut(s) 584
BbvI GCAGC 1 cut(s) 660
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 1 cut(s) 127
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 3 cut(s) 295, 358, 766
BfaI CTAG 6 cut(s) 80, 191, 287, 350, 563, 762
BfoI RGCGCY 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 775
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 5 cut(s) 36, 182, 549, 674, 738
BlsI GCNGC 5 cut(s) 37, 183, 550, 675, 739
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 127, 329
Bme18I GGWCC 3 cut(s) 166, 553, 600
BmgBI CACGTC 1 cut(s) 533
BmgT120I GGNCC 4 cut(s) 123, 166, 553, 600
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 127, 329
BmrI ACTGGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 60, 322
BmuI ACTGGG 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 683, 715
BpmI CTGGAG 1 cut(s) 707
Bpu14I TTCGAA 2 cut(s) 657, 667
BpuMI CCSGG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 849
BsaJI CCNNGG 2 cut(s) 126, 702
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 4 cut(s) 267, 281, 373, 690
Bse3DI GCAATG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 126, 702
BseGI GGATG 2 cut(s) 576, 635
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 439
BseNI ACTGG 4 cut(s) 267, 281, 373, 690
BsePI GCGCGC 1 cut(s) 70
BseRI GAGGAG 4 cut(s) 283, 346, 457, 704
BseXI GCAGC 1 cut(s) 660
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 505
Bsh1236I CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
Bsh1285I CGRYCG 2 cut(s) 67, 336
BshFI GGCC 3 cut(s) 38, 112, 125
BsiEI CGRYCG 2 cut(s) 67, 336
BsiSI CCGG 2 cut(s) 67, 329
BslFI GGGAC 2 cut(s) 68, 518
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 3 cut(s) 295, 358, 766
BsmFI GGGAC 2 cut(s) 68, 518
BsnI GGCC 3 cut(s) 38, 112, 125
Bsp119I TTCGAA 2 cut(s) 657, 667
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 321, 588
Bsp19I CCATGG 1 cut(s) 702
Bsp68I TCGCGA 2 cut(s) 298, 361
BspACI CCGC 8 cut(s) 35, 181, 274, 278, 527, 548, 551, 737
BspANI GGCC 3 cut(s) 38, 112, 125
BspCNI CTCAG 1 cut(s) 438
BspFNI CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
BspLI GGNNCC 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 775
BspT104I TTCGAA 2 cut(s) 657, 667
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 4 cut(s) 267, 281, 373, 690
BssAI RCCGGY 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 126, 702
BssHII GCGCGC 1 cut(s) 70
BssMI GATC 2 cut(s) 321, 588
BssSI CACGAG 1 cut(s) 584
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 308, 337
Bst6I CTCTTC 2 cut(s) 590, 764
BstBI TTCGAA 2 cut(s) 657, 667
BstC8I GCNNGC 5 cut(s) 72, 110, 278, 300, 363
BstDEI CTNAG 1 cut(s) 425
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 2 cut(s) 576, 635
BstFNI CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 3 cut(s) 72, 74, 481
BstKTI GATC 2 cut(s) 324, 591
BstMAI GTCTC 3 cut(s) 295, 358, 766
BstMBI GATC 2 cut(s) 321, 588
BstMCI CGRYCG 2 cut(s) 67, 336
BstMWI GCNNNNNNNGC 3 cut(s) 69, 131, 159
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 125, 327
BstUI CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
BstV1I GCAGC 1 cut(s) 660
BsuRI GGCC 3 cut(s) 38, 112, 125
BtgI CCRYGG 1 cut(s) 702
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 2 cut(s) 576, 635
BtsIMutI CAGTG 2 cut(s) 274, 683
BtuMI TCGCGA 2 cut(s) 298, 361
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 5 cut(s) 72, 110, 278, 300, 363
CfoI GCGC 3 cut(s) 72, 74, 481
Cfr10I RCCGGY 1 cut(s) 66
Cfr13I GGNCC 4 cut(s) 123, 166, 553, 600
CpoI CGGWCCG 1 cut(s) 553
CseI GACGC 2 cut(s) 41, 554
Csp6I GTAC 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CspI CGGWCCG 1 cut(s) 553
CviAII CATG 1 cut(s) 703
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 425
DpnI GATC 2 cut(s) 323, 590
DpnII GATC 2 cut(s) 321, 588
Eam1104I CTCTTC 2 cut(s) 590, 764
EarI CTCTTC 2 cut(s) 590, 764
EciI GGCGGA 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 702
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 3 cut(s) 166, 553, 600
Eco47III AGCGCT 1 cut(s) 480
EcoO109I RGGNCCY 3 cut(s) 123, 166, 600
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 1 cut(s) 706
FaiI YATR 7 cut(s) 641, 704, 750, 759, 782, 804, 856
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 2 cut(s) 68, 518
FatI CATG 1 cut(s) 702
FauI CCCGC 2 cut(s) 285, 534
Fnu4HI GCNGC 5 cut(s) 36, 182, 549, 674, 738
FokI GGATG 2 cut(s) 563, 622
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 5 cut(s) 36, 182, 549, 674, 738
FspBI CTAG 6 cut(s) 80, 191, 287, 350, 563, 762
GlaI GCGC 3 cut(s) 71, 73, 480
GluI GCNGC 5 cut(s) 36, 182, 549, 674, 738
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 1 cut(s) 707
HaeII RGCGCY 1 cut(s) 482
HaeIII GGCC 3 cut(s) 38, 112, 125
HapII CCGG 2 cut(s) 67, 329
HgaI GACGC 2 cut(s) 41, 554
HhaI GCGC 3 cut(s) 72, 74, 481
Hin1II CATG 1 cut(s) 706
Hin6I GCGC 3 cut(s) 70, 72, 479
HinP1I GCGC 3 cut(s) 70, 72, 479
HinfI GANTC 1 cut(s) 558
HpaII CCGG 2 cut(s) 67, 329
HphI GGTGA 4 cut(s) 308, 529, 799, 854
Hpy166II GTNNAC 2 cut(s) 474, 790
Hpy188I TCNGA 2 cut(s) 428, 544
Hpy188III TCNNGA 4 cut(s) 297, 360, 586, 592
Hpy8I GTNNAC 2 cut(s) 474, 790
Hpy99I CGWCG 3 cut(s) 534, 548, 572
HpyAV CCTTC 4 cut(s) 49, 97, 258, 423
HpyCH4III ACNGT 2 cut(s) 308, 337
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 3 cut(s) 522, 676, 784
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 131, 159
HpyF3I CTNAG 1 cut(s) 425
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 1 cut(s) 706
HspAI GCGC 3 cut(s) 70, 72, 479
Kzo9I GATC 2 cut(s) 321, 588
LmnI GCTCC 2 cut(s) 691, 704
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 2 cut(s) 60, 322
MaeI CTAG 6 cut(s) 80, 191, 287, 350, 563, 762
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 4 cut(s) 96, 269, 448, 535
MalI GATC 2 cut(s) 323, 590
MboI GATC 2 cut(s) 321, 588
MboII GAAGA 7 cut(s) 95, 451, 497, 500, 607, 656, 781
MhlI GDGCHC 1 cut(s) 147
MmeI TCCRAC 1 cut(s) 567
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 4 cut(s) 45, 177, 605, 865
MspI CCGG 2 cut(s) 67, 329
MspR9I CCNGG 2 cut(s) 127, 329
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 6 cut(s) 35, 72, 298, 361, 529, 548
MwoI GCNNNNNNNGC 3 cut(s) 69, 131, 159
NciI CCSGG 1 cut(s) 329
NcoI CCATGG 1 cut(s) 702
NdeII GATC 2 cut(s) 321, 588
NlaIII CATG 1 cut(s) 706
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 3 cut(s) 269, 448, 535
NruI TCGCGA 2 cut(s) 298, 361
NspV TTCGAA 2 cut(s) 657, 667
PalAI GGCGCGCC 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 775
PauI GCGCGC 1 cut(s) 70
PceI AGGCCT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 558
PkrI GCNGC 5 cut(s) 37, 183, 550, 675, 739
PpuMI RGGWCCY 2 cut(s) 166, 600
PshBI ATTAAT 1 cut(s) 45
Psp5II RGGWCCY 2 cut(s) 166, 600
Psp6I CCWGG 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 4 cut(s) 123, 166, 553, 600
PspPPI RGGWCCY 2 cut(s) 166, 600
PteI GCGCGC 1 cut(s) 70
RruI TCGCGA 2 cut(s) 298, 361
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
Rsr2I CGGWCCG 1 cut(s) 553
RsrII CGGWCCG 1 cut(s) 553
SaqAI TTAA 4 cut(s) 45, 177, 605, 865
SatI GCNGC 5 cut(s) 36, 182, 549, 674, 738
Sau3AI GATC 2 cut(s) 321, 588
Sau96I GGNCC 4 cut(s) 123, 166, 553, 600
ScaI AGTACT 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 127, 329
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 2 cut(s) 60, 322
SfuI TTCGAA 2 cut(s) 657, 667
SgsI GGCGCGCC 1 cut(s) 70
SinI GGWCC 3 cut(s) 166, 553, 600
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 8 cut(s) 35, 181, 274, 278, 527, 548, 551, 737
SspMI CTAG 6 cut(s) 80, 191, 287, 350, 563, 762
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 125, 327
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 2 cut(s) 308, 337
TaiI ACGT 1 cut(s) 535
TaqI TCGA 3 cut(s) 657, 667, 732
TaqII GACCGA 1 cut(s) 570
TatI WGTACW 1 cut(s) 375
TauI GCSGC 4 cut(s) 38, 184, 551, 740
TfiI GAWTC 1 cut(s) 558
Tru1I TTAA 4 cut(s) 45, 177, 605, 865
Tru9I TTAA 4 cut(s) 45, 177, 605, 865
TscAI CASTG 2 cut(s) 274, 690
TseFI GTSAC 3 cut(s) 269, 448, 535
TseI GCWGC 1 cut(s) 673
Tsp45I GTSAC 3 cut(s) 269, 448, 535
TspDTI ATGAA 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 757
TspRI CASTG 2 cut(s) 274, 690
VpaK11BI GGWCC 3 cut(s) 166, 553, 600
VspI ATTAAT 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 6 cut(s) 80, 191, 287, 350, 563, 762
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.