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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 94, 157 |
| AciI | CCGC | 4 cut(s) | 70, 74, 155, 178 |
| AgsI | TTSAA | 3 cut(s) | 48, 58, 212 |
| Alw26I | GTCTC | 3 cut(s) | 91, 154, 287 |
| AspLEI | GCGC | 1 cut(s) | 208 |
| AsuC2I | CCSGG | 2 cut(s) | 63, 125 |
| AsuHPI | GGTGA | 4 cut(s) | 28, 77, 104, 139 |
| BcnI | CCSGG | 2 cut(s) | 63, 125 |
| BcoDI | GTCTC | 3 cut(s) | 91, 154, 287 |
| BfaI | CTAG | 2 cut(s) | 83, 146 |
| BfoI | RGCGCY | 1 cut(s) | 209 |
| Bme1390I | CCNGG | 2 cut(s) | 63, 125 |
| BmiI | GGNNCC | 1 cut(s) | 322 |
| BmrFI | CCNGG | 2 cut(s) | 63, 125 |
| BmsI | GCATC | 1 cut(s) | 118 |
| BpuMI | CCSGG | 2 cut(s) | 63, 125 |
| BsaJI | CCNNGG | 1 cut(s) | 254 |
| Bse1I | ACTGG | 4 cut(s) | 77, 140, 169, 316 |
| BseDI | CCNNGG | 1 cut(s) | 254 |
| BseNI | ACTGG | 4 cut(s) | 77, 140, 169, 316 |
| BseRI | GAGGAG | 1 cut(s) | 79 |
| BsgI | GTGCAG | 1 cut(s) | 232 |
| Bsh1236I | CGCG | 2 cut(s) | 94, 157 |
| BsiSI | CCGG | 2 cut(s) | 63, 125 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BsmAI | GTCTC | 3 cut(s) | 91, 154, 287 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| Bsp143I | GATC | 1 cut(s) | 117 |
| Bsp68I | TCGCGA | 1 cut(s) | 94 |
| BspACI | CCGC | 4 cut(s) | 70, 74, 155, 178 |
| BspFNI | CGCG | 2 cut(s) | 94, 157 |
| BspLI | GGNNCC | 1 cut(s) | 322 |
| BsrI | ACTGG | 4 cut(s) | 77, 140, 169, 316 |
| BssECI | CCNNGG | 1 cut(s) | 254 |
| BssMI | GATC | 1 cut(s) | 117 |
| Bst4CI | ACNGT | 3 cut(s) | 104, 258, 298 |
| BstC8I | GCNNGC | 7 cut(s) | 30, 74, 96, 159, 263, 267, 305 |
| BstDSI | CCRYGG | 1 cut(s) | 254 |
| BstEII | GGTNACC | 1 cut(s) | 65 |
| BstFNI | CGCG | 2 cut(s) | 94, 157 |
| BstH2I | RGCGCY | 1 cut(s) | 209 |
| BstHHI | GCGC | 1 cut(s) | 208 |
| BstKTI | GATC | 1 cut(s) | 120 |
| BstMAI | GTCTC | 3 cut(s) | 91, 154, 287 |
| BstMBI | GATC | 1 cut(s) | 117 |
| BstPI | GGTNACC | 1 cut(s) | 65 |
| BstSCI | CCNGG | 2 cut(s) | 61, 123 |
| BstUI | CGCG | 2 cut(s) | 94, 157 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 261 |
| BtgI | CCRYGG | 1 cut(s) | 254 |
| BtsIMutI | CAGTG | 1 cut(s) | 176 |
| BtuMI | TCGCGA | 1 cut(s) | 94 |
| Cac8I | GCNNGC | 7 cut(s) | 30, 74, 96, 159, 263, 267, 305 |
| CfoI | GCGC | 1 cut(s) | 208 |
| CviJI | RGCY | 4 cut(s) | 28, 98, 161, 323 |
| CviKI_1 | RGCY | 4 cut(s) | 28, 98, 161, 323 |
| DpnI | GATC | 1 cut(s) | 119 |
| DpnII | GATC | 1 cut(s) | 117 |
| Eco91I | GGTNACC | 1 cut(s) | 65 |
| EcoO65I | GGTNACC | 1 cut(s) | 65 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FauI | CCCGC | 2 cut(s) | 81, 162 |
| FspBI | CTAG | 2 cut(s) | 83, 146 |
| GlaI | GCGC | 1 cut(s) | 207 |
| HaeII | RGCGCY | 1 cut(s) | 209 |
| HapII | CCGG | 2 cut(s) | 63, 125 |
| HhaI | GCGC | 1 cut(s) | 208 |
| Hin6I | GCGC | 1 cut(s) | 206 |
| HinP1I | GCGC | 1 cut(s) | 206 |
| HpaII | CCGG | 2 cut(s) | 63, 125 |
| HphI | GGTGA | 4 cut(s) | 28, 77, 104, 139 |
| Hpy188I | TCNGA | 1 cut(s) | 36 |
| Hpy188III | TCNNGA | 1 cut(s) | 93 |
| Hpy99I | CGWCG | 1 cut(s) | 245 |
| HpyAV | CCTTC | 1 cut(s) | 54 |
| HpyCH4III | ACNGT | 3 cut(s) | 104, 258, 298 |
| HpyCH4V | TGCA | 2 cut(s) | 249, 303 |
| HspAI | GCGC | 1 cut(s) | 206 |
| Kzo9I | GATC | 1 cut(s) | 117 |
| LmnI | GCTCC | 2 cut(s) | 36, 320 |
| LweI | GCATC | 1 cut(s) | 118 |
| MaeI | CTAG | 2 cut(s) | 83, 146 |
| MaeIII | GTNAC | 2 cut(s) | 65, 173 |
| MalI | GATC | 1 cut(s) | 119 |
| MboI | GATC | 1 cut(s) | 117 |
| MboII | GAAGA | 3 cut(s) | 20, 224, 227 |
| MnlI | CCTC | 1 cut(s) | 100 |
| MspI | CCGG | 2 cut(s) | 63, 125 |
| MspR9I | CCNGG | 2 cut(s) | 63, 125 |
| MvnI | CGCG | 2 cut(s) | 94, 157 |
| NciI | CCSGG | 2 cut(s) | 63, 125 |
| NdeII | GATC | 1 cut(s) | 117 |
| NlaIV | GGNNCC | 1 cut(s) | 322 |
| NmuCI | GTSAC | 1 cut(s) | 65 |
| NruI | TCGCGA | 1 cut(s) | 94 |
| PspEI | GGTNACC | 1 cut(s) | 65 |
| PspN4I | GGNNCC | 1 cut(s) | 322 |
| RruI | TCGCGA | 1 cut(s) | 94 |
| Sau3AI | GATC | 1 cut(s) | 117 |
| ScrFI | CCNGG | 2 cut(s) | 63, 125 |
| SetI | ASST | 2 cut(s) | 46, 227 |
| SfaNI | GCATC | 1 cut(s) | 118 |
| SsiI | CCGC | 4 cut(s) | 70, 74, 155, 178 |
| SspMI | CTAG | 2 cut(s) | 83, 146 |
| StyD4I | CCNGG | 2 cut(s) | 61, 123 |
| TaaI | ACNGT | 3 cut(s) | 104, 258, 298 |
| TscAI | CASTG | 1 cut(s) | 176 |
| TseFI | GTSAC | 1 cut(s) | 65 |
| Tsp45I | GTSAC | 1 cut(s) | 65 |
| TspRI | CASTG | 1 cut(s) | 176 |
| XspI | CTAG | 2 cut(s) | 83, 146 |