Rmu_sc0009454.1_g000015

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009454.1
OG0002038
Physical Location & Seq
Reverse (-)
53341 .. 56488
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009454.1_g000015.1.cds

Sequence Viewer

Length: 1098 bp
atgtgtatccgtgcattcaagaaactaaagtcattgccagatgagatgcacacctatcttccttctcttcagaatatgatcctagaagattgtccagagctagaatcatttcctatagggggtttgccgtcaaagttaaaaacacttgactttgattgtagcaaaaagctcttggaaaagaagcaatgggctctaccaagactcaacatcctcagaagcttaaaactcggcttcaacggatatgaagggacttcatttccactggaggagggactgttcccaacccagctcgcttctttctctttcttcaaagttcaatctgaagaatgtcacgacgatcaaaatcaaggagttaacctcaactctcttgaaaagctgtttattggggaatgccccaagttgaggaacttacctccggatgggttacctgcttctctttatcatctgggaatcgagaattttccttggttaaatggagggcacatgagaatgacacgggagaattgcccccaggagggaggaacagttggatgtgttagaaatgaatccttgcatccttctggtgaccgaaaaattgtaattggtaataggtactgtttgagcccaaaagtatttttggcaggatcctctggtgggtttgagccagcgggccgatacctgcggcccaaaaataagcctactcgggtttgggggttaagcggcaaagttaaaggacctttctcgaatcaatcaatcccagcgattacaaagcctccccggagcaaaccttcaacactgttgaaacccggtgaccgtactctcagtcctagtctctcagagccgactgtcagtgctactgccacagatctcccagtgaagcaagggcaaaagtcctttcccgagaggctagagaagaaccctgtggcgaggttagtgctctcctcgtccacagcgcttgaagaagaagtcaggtcaagggactaccccaacgactgtaccccacggtgctggcacgcctgcgcaatcacaggctcaaaagagacagtttgcacaccaacttgttttggagccaaacattttggcacgcccagtgggaccaacaatagtgtctccttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

365

Amino Acids

40.22

Weight (kDa)

8.96

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1000
Acc36I ACCTGC 2 cut(s) 436, 666
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 3 cut(s) 647, 661, 699
AclWI GGATC 3 cut(s) 73, 618, 631
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 2 cut(s) 53, 342
AfaI GTAC 3 cut(s) 593, 796, 976
AfeI AGCGCT 1 cut(s) 933
AfiI CCNNNNNNNGG 6 cut(s) 119, 402, 419, 514, 515, 633
AgsI TTSAA 8 cut(s) 19, 235, 310, 317, 371, 771, 781, 938
AjnI CCWGG 1 cut(s) 510
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 5 cut(s) 100, 169, 219, 289, 376
AluI AGCT 5 cut(s) 100, 169, 219, 289, 376
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 3 cut(s) 815, 1013, 1093
AlwI GGATC 3 cut(s) 73, 618, 631
Ama87I CYCGRG 2 cut(s) 681, 878
Aor13HI TCCGGA 1 cut(s) 415
Aor51HI AGCGCT 1 cut(s) 933
AoxI GGCC 2 cut(s) 649, 662
ApoI RAATTY 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 2 cut(s) 934, 1001
AspS9I GGNCC 4 cut(s) 649, 663, 713, 1074
AsuC2I CCSGG 2 cut(s) 757, 786
AsuHPI GGTGA 2 cut(s) 575, 800
AvaI CYCGRG 2 cut(s) 681, 878
AvaII GGWCC 2 cut(s) 713, 1074
BaeGI GKGCMC 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BamHI GGATCC 1 cut(s) 623
BanII GRGCYC 2 cut(s) 193, 605
Bbv12I GWGCWC 1 cut(s) 918
BccI CCATC 1 cut(s) 413
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciT130I CCWGG 1 cut(s) 512
BciVI GTATCC 1 cut(s) 17
BcnI CCSGG 2 cut(s) 757, 786
BcoDI GTCTC 3 cut(s) 815, 1013, 1093
BfaI CTAG 4 cut(s) 83, 101, 807, 887
BfmI CTRYAG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 935
BfuAI ACCTGC 2 cut(s) 436, 666
BfuI GTATCC 1 cut(s) 17
BglII AGATCT 1 cut(s) 844
BisI GCNGC 2 cut(s) 662, 700
BlsI GCNGC 2 cut(s) 663, 701
Bme1390I CCNGG 3 cut(s) 512, 757, 786
Bme18I GGWCC 2 cut(s) 713, 1074
BmeT110I CYCGRG 2 cut(s) 681, 878
BmgT120I GGNCC 4 cut(s) 649, 663, 713, 1074
BmiI GGNNCC 3 cut(s) 625, 1048, 1075
BmrFI CCNGG 3 cut(s) 512, 757, 786
BmrI ACTGGG 2 cut(s) 845, 1062
BmsI GCATC 2 cut(s) 36, 562
BmuI ACTGGG 2 cut(s) 845, 1062
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpmI CTGGAG 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 757, 786
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 4 cut(s) 464, 510, 755, 980
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 6 cut(s) 260, 290, 510, 540, 736, 766
Bsc4I CCNNNNNNNGG 6 cut(s) 119, 402, 419, 514, 515, 633
Bse1I ACTGG 3 cut(s) 267, 851, 1068
Bse3DI GCAATG 2 cut(s) 32, 191
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 4 cut(s) 464, 510, 755, 980
BseGI GGATG 4 cut(s) 207, 424, 536, 553
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 6 cut(s) 119, 402, 419, 514, 515, 633
BseMI GCAATG 2 cut(s) 32, 191
BseMII CTCAG 3 cut(s) 226, 814, 828
BseNI ACTGG 3 cut(s) 267, 851, 1068
BseRI GAGGAG 2 cut(s) 281, 910
BseSI GKGCMC 1 cut(s) 483
BseYI CCCAGC 2 cut(s) 285, 736
BshFI GGCC 2 cut(s) 651, 664
BsiHKAI GWGCWC 1 cut(s) 918
BsiHKCI CYCGRG 2 cut(s) 681, 878
BsiSI CCGG 3 cut(s) 416, 757, 786
BslFI GGGAC 4 cut(s) 262, 285, 971, 1087
BslI CCNNNNNNNGG 6 cut(s) 119, 402, 419, 514, 515, 633
BsmAI GTCTC 3 cut(s) 815, 1013, 1093
BsmFI GGGAC 4 cut(s) 262, 285, 971, 1087
BsmI GAATGC 2 cut(s) 14, 395
BsnI GGCC 2 cut(s) 651, 664
BsoBI CYCGRG 2 cut(s) 681, 878
Bsp1286I GDGCHC 4 cut(s) 193, 483, 605, 918
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 4 cut(s) 78, 337, 623, 844
BspACI CCGC 3 cut(s) 647, 661, 699
BspANI GGCC 2 cut(s) 651, 664
BspCNI CTCAG 3 cut(s) 225, 813, 827
BspEI TCCGGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 625, 1048, 1075
BspMI ACCTGC 2 cut(s) 436, 666
BspPI GGATC 3 cut(s) 73, 618, 631
BsrDI GCAATG 2 cut(s) 32, 191
BsrI ACTGG 3 cut(s) 267, 851, 1068
BssECI CCNNGG 4 cut(s) 464, 510, 755, 980
BssMI GATC 4 cut(s) 78, 337, 623, 844
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 9 cut(s) 276, 526, 596, 777, 794, 826, 974, 984, 1024
Bst6I CTCTTC 1 cut(s) 72
BstC8I GCNNGC 7 cut(s) 291, 645, 649, 989, 993, 997, 1064
BstDEI CTNAG 3 cut(s) 212, 800, 814
BstDSI CCRYGG 1 cut(s) 980
BstEII GGTNACC 3 cut(s) 423, 563, 788
BstF5I GGATG 4 cut(s) 207, 424, 536, 553
BstH2I RGCGCY 1 cut(s) 935
BstHHI GCGC 2 cut(s) 934, 1001
BstKTI GATC 4 cut(s) 81, 340, 626, 847
BstMAI GTCTC 3 cut(s) 815, 1013, 1093
BstMBI GATC 4 cut(s) 78, 337, 623, 844
BstNI CCWGG 1 cut(s) 512
BstPI GGTNACC 3 cut(s) 423, 563, 788
BstSCI CCNGG 3 cut(s) 510, 755, 784
BstSFI CTRYAG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 483
BstX2I RGATCY 2 cut(s) 623, 844
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 2 cut(s) 623, 844
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 2 cut(s) 651, 664
BtgI CCRYGG 1 cut(s) 980
BtsCI GGATG 4 cut(s) 207, 424, 536, 553
BtsIMutI CAGTG 5 cut(s) 260, 773, 835, 858, 1075
BveI ACCTGC 2 cut(s) 436, 666
Cac8I GCNNGC 7 cut(s) 291, 645, 649, 989, 993, 997, 1064
CfoI GCGC 2 cut(s) 934, 1001
Cfr13I GGNCC 4 cut(s) 649, 663, 713, 1074
Csp6I GTAC 3 cut(s) 592, 795, 975
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviAII CATG 1 cut(s) 484
CviQI GTAC 3 cut(s) 592, 795, 975
DdeI CTNAG 3 cut(s) 212, 800, 814
DpnI GATC 4 cut(s) 80, 339, 625, 846
DpnII GATC 4 cut(s) 78, 337, 623, 844
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 193, 605
Eco47I GGWCC 2 cut(s) 713, 1074
Eco47III AGCGCT 1 cut(s) 933
Eco57I CTGAAG 2 cut(s) 53, 342
Eco88I CYCGRG 2 cut(s) 681, 878
Eco91I GGTNACC 3 cut(s) 423, 563, 788
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 3 cut(s) 423, 563, 788
EcoRII CCWGG 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 464
EcoT38I GRGCYC 2 cut(s) 193, 605
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 1 cut(s) 487
FaiI YATR 4 cut(s) 77, 116, 243, 485
FaqI GGGAC 4 cut(s) 262, 285, 971, 1087
FatI CATG 1 cut(s) 483
FauI CCCGC 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 662, 700
FokI GGATG 4 cut(s) 194, 431, 540, 543
FriOI GRGCYC 2 cut(s) 193, 605
Fsp4HI GCNGC 2 cut(s) 662, 700
FspBI CTAG 4 cut(s) 83, 101, 807, 887
FspI TGCGCA 1 cut(s) 1000
GlaI GCGC 2 cut(s) 933, 1000
GluI GCNGC 2 cut(s) 662, 700
GsaI CCCAGC 2 cut(s) 289, 740
GsuI CTGGAG 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 935
HaeIII GGCC 2 cut(s) 651, 664
HapII CCGG 3 cut(s) 416, 757, 786
HhaI GCGC 2 cut(s) 934, 1001
Hin1II CATG 1 cut(s) 487
Hin6I GCGC 2 cut(s) 932, 999
HinP1I GCGC 2 cut(s) 932, 999
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 217
HinfI GANTC 5 cut(s) 104, 201, 450, 545, 724
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 3 cut(s) 416, 757, 786
HphI GGTGA 2 cut(s) 575, 800
Hpy166II GTNNAC 2 cut(s) 355, 927
Hpy188I TCNGA 4 cut(s) 72, 215, 322, 817
Hpy188III TCNNGA 8 cut(s) 19, 95, 332, 368, 416, 454, 721, 878
Hpy8I GTNNAC 2 cut(s) 355, 927
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 4 cut(s) 72, 239, 567, 777
HpyCH4III ACNGT 9 cut(s) 276, 526, 596, 777, 794, 826, 974, 984, 1024
HpyCH4V TGCA 4 cut(s) 14, 49, 553, 1029
HpyF3I CTNAG 3 cut(s) 212, 800, 814
Hsp92II CATG 1 cut(s) 487
HspAI GCGC 2 cut(s) 932, 999
Kpn2I TCCGGA 1 cut(s) 415
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 4 cut(s) 78, 337, 623, 844
LmnI GCTCC 2 cut(s) 759, 1046
LweI GCATC 2 cut(s) 36, 562
MaeI CTAG 4 cut(s) 83, 101, 807, 887
MaeIII GTNAC 4 cut(s) 329, 423, 563, 788
MalI GATC 4 cut(s) 80, 339, 625, 846
MboI GATC 4 cut(s) 78, 337, 623, 844
MboII GAAGA 8 cut(s) 50, 59, 98, 298, 335, 904, 950, 953
MflI RGATCY 2 cut(s) 623, 844
MhlI GDGCHC 4 cut(s) 193, 483, 605, 918
MluCI AATT 4 cut(s) 457, 502, 573, 579
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 508
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 6 cut(s) 137, 221, 354, 470, 695, 708
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 3 cut(s) 416, 757, 786
MspR9I CCNGG 3 cut(s) 512, 757, 786
Mva1269I GAATGC 2 cut(s) 14, 395
MvaI CCWGG 1 cut(s) 512
NciI CCSGG 2 cut(s) 757, 786
NdeII GATC 4 cut(s) 78, 337, 623, 844
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 3 cut(s) 625, 1048, 1075
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 3 cut(s) 329, 563, 788
NsbI TGCGCA 1 cut(s) 1000
PctI GAATGC 2 cut(s) 14, 395
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 4 cut(s) 104, 450, 545, 724
PkrI GCNGC 2 cut(s) 663, 701
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PpuMI RGGWCCY 1 cut(s) 713
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 510
PspEI GGTNACC 3 cut(s) 423, 563, 788
PspFI CCCAGC 2 cut(s) 285, 736
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 3 cut(s) 625, 1048, 1075
PspPI GGNCC 4 cut(s) 649, 663, 713, 1074
PspPPI RGGWCCY 1 cut(s) 713
PsuI RGATCY 2 cut(s) 623, 844
RsaI GTAC 3 cut(s) 593, 796, 976
RsaNI GTAC 3 cut(s) 592, 795, 975
RseI CAYNNNNRTG 1 cut(s) 488
SaqAI TTAA 6 cut(s) 137, 221, 354, 470, 695, 708
SatI GCNGC 2 cut(s) 662, 700
Sau3AI GATC 4 cut(s) 78, 337, 623, 844
Sau96I GGNCC 4 cut(s) 649, 663, 713, 1074
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 3 cut(s) 512, 757, 786
SduI GDGCHC 4 cut(s) 193, 483, 605, 918
SfaNI GCATC 2 cut(s) 36, 562
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 2 cut(s) 713, 1074
SmiMI CAYNNNNRTG 1 cut(s) 488
Sse9I AATT 4 cut(s) 457, 502, 573, 579
SsiI CCGC 3 cut(s) 647, 661, 699
SspMI CTAG 4 cut(s) 83, 101, 807, 887
StyD4I CCNGG 3 cut(s) 510, 755, 784
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 9 cut(s) 276, 526, 596, 777, 794, 826, 974, 984, 1024
TaqI TCGA 2 cut(s) 453, 722
TaqII GACCGA 1 cut(s) 582
TasI AATT 4 cut(s) 457, 502, 573, 579
TauI GCSGC 2 cut(s) 664, 702
TfiI GAWTC 4 cut(s) 104, 450, 545, 724
Tru1I TTAA 6 cut(s) 137, 221, 354, 470, 695, 708
Tru9I TTAA 6 cut(s) 137, 221, 354, 470, 695, 708
TscAI CASTG 5 cut(s) 267, 780, 835, 858, 1075
TseFI GTSAC 3 cut(s) 329, 563, 788
Tsp45I GTSAC 3 cut(s) 329, 563, 788
TspDTI ATGAA 3 cut(s) 243, 258, 558
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 5 cut(s) 267, 780, 835, 858, 1075
VpaK11BI GGWCC 2 cut(s) 713, 1074
XapI RAATTY 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 4 cut(s) 83, 101, 807, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.