Rmu_sc0003395.1_g000004

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003395.1
OG0001406
Physical Location & Seq
Reverse (-)
8464 .. 9797
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
atggctggtgtcgaggatgtgacttcgaagcttgcaacggccctagctattgggggagatgacttccatcgtgacgatccagaggtggaattgacaaatctatcctaccattttttggtgggtcatctggtgtcggtggagccaacgaaagcggcagtgctccgattcatggctcaaattagccagatttggcccatggctgaacatagtttggtgatccgtgaatgcccgaatgatcgatttctcttccgatttgttaacccgacggataagcagcgggatttggctaaatctccatggtactactgttcggctactctggttttaagggattatgatagggtgggtgagatcatggatgtgcctctgaatcggctgctgatctgggttcaaatcagagggatgtcgccagcctataccacaccgaattgggctcgaaaggttgctgggtgtttgggtcgggttgttcatgtagatgaaaggagggtgaagcgtcataaggagggaggttcatcgatggtggtggaggttgttacccatcctagtgaggtaagagtgccatcgggttttgctgctttatcggaactcaagcaggtgggtgaccattctggggttggtccattattgttagaaggtaaggtggactctctgcctcgggtttcgaaagagagtgttgatgaggtgcagaccatggttgggtcgatgcttggaaaagtcacttccttattgaaaaaagggagattgggatctggtgttgatccagggcacaaagactcacctggagcttctgtgggtggtgtgttatcggctcctatgagccgggattcgttgagtcatcggaagcgcaaggtggatgcttccacattcagttttggtatgggggcctcccctaccaaaggcctgcagtttggggtgggaaagtcttcatcccctactaatggtaagtttcgcgtccaagatcagcatcacttgatcggtgaagattatgtccaggctatgtggcaggagcatgaagaccttagcgataagctcttagaggctttggacagcttagtgatggaaaagcaacgcatcgctcttgcctacgataagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

364

Amino Acids

40.06

Weight (kDa)

7.24

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 37 - 112 1.5e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 583
Acc36I ACCTGC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 115
AccII CGCG 1 cut(s) 951
AciI CCGC 2 cut(s) 152, 277
AclWI GGATC 4 cut(s) 71, 211, 752, 754
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 6 cut(s) 115, 169, 610, 696, 896, 938
AgsI TTSAA 2 cut(s) 392, 730
AjnI CCWGG 3 cut(s) 760, 778, 990
AluBI AGCT 5 cut(s) 31, 47, 785, 1030, 1050
AluI AGCT 5 cut(s) 31, 47, 785, 1030, 1050
Alw21I GWGCWC 1 cut(s) 162
AlwI GGATC 4 cut(s) 71, 211, 752, 754
Ama87I CYCGRG 1 cut(s) 654
AoxI GGCC 4 cut(s) 39, 191, 882, 898
ApeKI GCWGC 3 cut(s) 274, 376, 572
Asp700I GAANNNNTTC 1 cut(s) 922
AspLEI GCGC 1 cut(s) 846
AspS9I GGNCC 4 cut(s) 40, 192, 617, 882
AsuC2I CCSGG 1 cut(s) 821
AsuHPI GGTGA 6 cut(s) 226, 359, 499, 611, 768, 989
AsuII TTCGAA 2 cut(s) 26, 662
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 768
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BanII GRGCYC 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 915, 1020
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 286, 363, 559
BccI CCATC 5 cut(s) 75, 511, 546, 568, 1051
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 3 cut(s) 762, 780, 992
BcnI CCSGG 1 cut(s) 821
BfaI CTAG 2 cut(s) 44, 543
BfmI CTRYAG 1 cut(s) 902
BfuAI ACCTGC 1 cut(s) 583
BisI GCNGC 4 cut(s) 153, 275, 377, 573
BlsI GCNGC 4 cut(s) 154, 276, 378, 574
Bme1390I CCNGG 4 cut(s) 762, 780, 821, 992
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 4 cut(s) 40, 192, 617, 882
BmiI GGNNCC 3 cut(s) 141, 810, 883
BmrFI CCNGG 4 cut(s) 762, 780, 821, 992
BmsI GCATC 4 cut(s) 693, 844, 973, 1080
BpiI GAAGAC 2 cut(s) 915, 1020
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 1 cut(s) 1019
Bpu14I TTCGAA 2 cut(s) 26, 662
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 1 cut(s) 821
Bsa29I ATCGAT 2 cut(s) 238, 515
BsaBI GATNNNNATC 2 cut(s) 745, 963
BsaJI CCNNGG 5 cut(s) 195, 296, 653, 690, 761
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 169, 610, 696, 896, 938
Bse8I GATNNNNATC 2 cut(s) 745, 963
BseBI CCWGG 3 cut(s) 762, 780, 992
BseCI ATCGAT 2 cut(s) 238, 515
BseDI CCNNGG 5 cut(s) 195, 296, 653, 690, 761
BseGI GGATG 6 cut(s) 22, 364, 408, 538, 859, 926
BseJI GATNNNNATC 2 cut(s) 745, 963
BseLI CCNNNNNNNGG 6 cut(s) 115, 169, 610, 696, 896, 938
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 3 cut(s) 286, 363, 559
BseYI CCCAGC 1 cut(s) 446
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 951
BshFI GGCC 4 cut(s) 41, 193, 884, 900
BshVI ATCGAT 2 cut(s) 238, 515
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 1 cut(s) 820
BslI CCNNNNNNNGG 6 cut(s) 115, 169, 610, 696, 896, 938
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 4 cut(s) 41, 193, 884, 900
BsoBI CYCGRG 1 cut(s) 654
Bsp119I TTCGAA 2 cut(s) 26, 662
Bsp1286I GDGCHC 3 cut(s) 162, 436, 768
Bsp143I GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
Bsp19I CCATGG 3 cut(s) 195, 296, 690
BspACI CCGC 2 cut(s) 152, 277
BspANI GGCC 4 cut(s) 41, 193, 884, 900
BspDI ATCGAT 2 cut(s) 238, 515
BspFNI CGCG 1 cut(s) 951
BspLI GGNNCC 3 cut(s) 141, 810, 883
BspMAI CTGCAG 1 cut(s) 906
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 4 cut(s) 71, 211, 752, 754
BspT104I TTCGAA 2 cut(s) 26, 662
BssECI CCNNGG 5 cut(s) 195, 296, 653, 690, 761
BssMI GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
BssT1I CCWWGG 3 cut(s) 195, 296, 690
Bst2UI CCWGG 3 cut(s) 762, 780, 992
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 251
BstBI TTCGAA 2 cut(s) 26, 662
BstC8I GCNNGC 3 cut(s) 33, 411, 902
BstDEI CTNAG 3 cut(s) 1019, 1033, 1051
BstDSI CCRYGG 3 cut(s) 195, 296, 690
BstEII GGTNACC 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 894
BstF5I GGATG 6 cut(s) 22, 364, 408, 538, 859, 926
BstFNI CGCG 1 cut(s) 951
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 9 cut(s) 79, 219, 238, 354, 384, 749, 760, 961, 975
BstMBI GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
BstNI CCWGG 3 cut(s) 762, 780, 992
BstPI GGTNACC 1 cut(s) 599
BstSCI CCNGG 4 cut(s) 760, 778, 819, 990
BstSFI CTRYAG 1 cut(s) 902
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 3 cut(s) 286, 363, 559
BstV2I GAAGAC 2 cut(s) 915, 1020
BstX2I RGATCY 1 cut(s) 746
BstYI RGATCY 1 cut(s) 746
Bsu15I ATCGAT 2 cut(s) 238, 515
BsuRI GGCC 4 cut(s) 41, 193, 884, 900
BsuTUI ATCGAT 2 cut(s) 238, 515
BtgI CCRYGG 3 cut(s) 195, 296, 690
BtgZI GCGATG 1 cut(s) 1057
BtsCI GGATG 6 cut(s) 22, 364, 408, 538, 859, 926
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 3 cut(s) 33, 411, 902
CfoI GCGC 1 cut(s) 846
Cfr13I GGNCC 4 cut(s) 40, 192, 617, 882
ClaI ATCGAT 2 cut(s) 238, 515
CseI GACGC 2 cut(s) 482, 940
Csp6I GTAC 1 cut(s) 301
CviAII CATG 7 cut(s) 169, 196, 297, 355, 470, 691, 1010
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 3 cut(s) 1019, 1033, 1051
DpnI GATC 9 cut(s) 78, 218, 237, 353, 383, 748, 759, 960, 974
DpnII GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco130I CCWWGG 3 cut(s) 195, 296, 690
Eco147I AGGCCT 1 cut(s) 900
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 654
Eco91I GGTNACC 1 cut(s) 599
EcoNI CCTNNNNNAGG 1 cut(s) 894
EcoO109I RGGNCCY 1 cut(s) 882
EcoO65I GGTNACC 1 cut(s) 599
EcoRII CCWGG 3 cut(s) 760, 778, 990
EcoT14I CCWWGG 3 cut(s) 195, 296, 690
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 195, 296, 690
FaeI CATG 7 cut(s) 172, 199, 300, 358, 473, 694, 1013
FatI CATG 7 cut(s) 168, 195, 296, 354, 469, 690, 1009
FauI CCCGC 1 cut(s) 270
Fnu4HI GCNGC 4 cut(s) 153, 275, 377, 573
FokI GGATG 6 cut(s) 29, 371, 415, 525, 866, 913
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 4 cut(s) 153, 275, 377, 573
FspBI CTAG 2 cut(s) 44, 543
GlaI GCGC 1 cut(s) 845
GluI GCNGC 4 cut(s) 153, 275, 377, 573
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 4 cut(s) 41, 193, 884, 900
HapII CCGG 1 cut(s) 820
HgaI GACGC 2 cut(s) 482, 940
HhaI GCGC 1 cut(s) 846
Hin1II CATG 7 cut(s) 172, 199, 300, 358, 473, 694, 1013
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 6 cut(s) 165, 370, 644, 773, 824, 832
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 1 cut(s) 820
HphI GGTGA 6 cut(s) 226, 359, 499, 611, 768, 989
Hpy166II GTNNAC 2 cut(s) 259, 643
Hpy188I TCNGA 6 cut(s) 164, 251, 369, 398, 583, 840
Hpy188III TCNNGA 2 cut(s) 71, 80
Hpy8I GTNNAC 2 cut(s) 259, 643
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 626
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4V TGCA 3 cut(s) 35, 685, 904
HpyF3I CTNAG 3 cut(s) 1019, 1033, 1051
Hsp92II CATG 7 cut(s) 172, 199, 300, 358, 473, 694, 1013
HspAI GCGC 1 cut(s) 844
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
LmnI GCTCC 5 cut(s) 139, 165, 782, 814, 1006
Lsp1109I GCAGC 3 cut(s) 286, 363, 559
LweI GCATC 4 cut(s) 693, 844, 973, 1080
MaeI CTAG 2 cut(s) 44, 543
MaeIII GTNAC 5 cut(s) 19, 71, 532, 599, 715
MalI GATC 9 cut(s) 78, 218, 237, 353, 383, 748, 759, 960, 974
MboI GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
MboII GAAGA 4 cut(s) 238, 915, 992, 1025
MflI RGATCY 1 cut(s) 746
MhlI GDGCHC 3 cut(s) 162, 436, 768
MluCI AATT 3 cut(s) 89, 177, 427
MlyI GAGTC 3 cut(s) 638, 767, 841
MroXI GAANNNNTTC 1 cut(s) 922
MseI TTAA 2 cut(s) 258, 326
MslI CAYNNNNRTG 3 cut(s) 359, 474, 543
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 1 cut(s) 820
MspR9I CCNGG 4 cut(s) 762, 780, 821, 992
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 3 cut(s) 762, 780, 992
MvnI CGCG 1 cut(s) 951
NciI CCSGG 1 cut(s) 821
NcoI CCATGG 3 cut(s) 195, 296, 690
NdeII GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
NlaIII CATG 7 cut(s) 172, 199, 300, 358, 473, 694, 1013
NlaIV GGNNCC 3 cut(s) 141, 810, 883
NmuCI GTSAC 4 cut(s) 19, 71, 599, 715
NspV TTCGAA 2 cut(s) 26, 662
PaqCI CACCTGC 1 cut(s) 583
PceI AGGCCT 1 cut(s) 900
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 165, 370, 824
PflMI CCANNNNNTGG 1 cut(s) 115
PkrI GCNGC 4 cut(s) 154, 276, 378, 574
PleI GAGTC 3 cut(s) 638, 767, 840
PpsI GAGTC 3 cut(s) 638, 767, 840
Psp6I CCWGG 3 cut(s) 760, 778, 990
PspEI GGTNACC 1 cut(s) 599
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 3 cut(s) 760, 778, 990
PspN4I GGNNCC 3 cut(s) 141, 810, 883
PspPI GGNCC 4 cut(s) 40, 192, 617, 882
PstI CTGCAG 1 cut(s) 906
PsuI RGATCY 1 cut(s) 746
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 3 cut(s) 359, 474, 543
SaqAI TTAA 2 cut(s) 258, 326
SatI GCNGC 4 cut(s) 153, 275, 377, 573
Sau3AI GATC 9 cut(s) 76, 216, 235, 351, 381, 746, 757, 958, 972
Sau96I GGNCC 4 cut(s) 40, 192, 617, 882
SchI GAGTC 3 cut(s) 638, 767, 841
ScrFI CCNGG 4 cut(s) 762, 780, 821, 992
SduI GDGCHC 3 cut(s) 162, 436, 768
SfaNI GCATC 4 cut(s) 693, 844, 973, 1080
SfcI CTRYAG 1 cut(s) 902
SfuI TTCGAA 2 cut(s) 26, 662
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 3 cut(s) 359, 474, 543
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 3 cut(s) 89, 177, 427
SseBI AGGCCT 1 cut(s) 900
SsiI CCGC 2 cut(s) 152, 277
SspMI CTAG 2 cut(s) 44, 543
StuI AGGCCT 1 cut(s) 900
StyD4I CCNGG 4 cut(s) 760, 778, 819, 990
StyI CCWWGG 3 cut(s) 195, 296, 690
TaaI ACNGT 1 cut(s) 308
TaqI TCGA 7 cut(s) 12, 26, 238, 436, 515, 662, 701
TasI AATT 3 cut(s) 89, 177, 427
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 3 cut(s) 165, 370, 824
Tru1I TTAA 2 cut(s) 258, 326
Tru9I TTAA 2 cut(s) 258, 326
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 4 cut(s) 19, 71, 599, 715
TseI GCWGC 3 cut(s) 274, 376, 572
Tsp45I GTSAC 4 cut(s) 19, 71, 599, 715
TspDTI ATGAA 6 cut(s) 157, 458, 492, 501, 915, 1026
TspGWI ACGGA 2 cut(s) 209, 281
TspRI CASTG 1 cut(s) 162
Van91I CCANNNNNTGG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 617
XagI CCTNNNNNAGG 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 922
XspI CTAG 2 cut(s) 44, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.