Rorug05G0543700

histone deacetylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000538
Physical Location & Seq
Forward (+)
73163158 .. 73176637
13480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0543700.1

Sequence Viewer

Length: 5445 bp
ATGCATCTTTACAATGCTTGGCTTCCTCCACCTGTCGCCGAGGAGTCCATCAAGGAGAGGGACTCCTTCTCTCGCGTCGTCTCCTCCGTCAAGAACTCCTACCGCTCCGATGACCCTGATTCAGTCTATGCAACTCTCAAATGGGTCTCTGTCATTGACCTTTTCATCAAGGCAAAGAGTGAAGTGTCTATGGAAGATGTGAGTGGACTGATTGAATTTGGATTGGAGTTGTTTCGTGTATCGGAGAATAAGCTTTATGCACAGGTTAGGTGGGGAAATATTCTTGTGAAATTACTCAACAAGTATCGGAAGAAGTTGTCGTTGAAGGTAAAGTGGCGCCCTTTGTATGATACTCTGATTCATACACATTTCAGTAGGAATACAGGTCCTGAAGGCTGGAGATTGAGACAACGGCATTTTGAGGCTACTACTTCTCTTGTTCGGTCTTGCCGGAAGTTCTTTCCCCCAGGCTCTGCCGTTGAGATATGGTCTGAGTTTAGATCTCTGTTGGAAAATCCTTGGCATAACTCATCCTTTGAGGGATCTGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGAATTCTTTACACATGATTGGATCAAGGAGTTAATGGACCTGTGGGACTCGATACCAAATTGCCAATTTTGGAACAGTCAATGGACAGCTATTATAGCTCGTGTTGTAAAGAACTACAGATTTATTGATTGGGAGAGCTACTTGCCTACACTTTTCACTAGATTCTTAAATATGTTTGAGGTTCCTGTTGCAAATGGAAGTGGATCGTATCCCTTTTCTGTGGATGTTCCGAGAAACACAAGGTTCTTGTTCTCCAATAGAACAGTTACCCCAGCAAAGGGCATTGCAAAGTCAATAGTCTATCTATTGAAACCTGGTAGCTCGGTGCTGGAGCACTTTGAAAAATTGGTCAACCTTTTAGAACAATATTACCATCCTTCTAATGGCGGCCGCTGGACTTACTCATTAGAGCGTTTTTTGCTCCATTTGGTAATTTCATTTCAGAAGCGCTTACAGAATGAACAATTGAAAGTAAATAAAGATAGACAGGATGAACAGTATCTGGGAAAATCAGAAAGGCATTTCTTTGTTAAAGTGGTGCTGAAGTTAATTGATCGCGGTCAATATAGCAAGAATGAACATCTTTCTGAGACAGTAGCTGCTGCAACTTCAATTTTATCTTACGTGGAGCCTTCTCTAATCCTTCCTTTTGTTGCATCTCGCTTCCATATGGCATTGGAGACGATGACTGCCACCCACCAGTTGCAAGTTGCTGTGATGTCAGTAGCATTTGTTGGACGTTCACTATTCCTCGAATCCTTGTCAACCTCTACAGTAAAATCAATTGATATTGGTAGTGGTGAAGCTGGTGAAGAGTTCATTGAGCTACTGATGGTTTCGTTATCCAATGCATTACTTGGTATGGATGCGAATGATCCTCCTAAAACCTTGGCAACAATGCAATTAATTGGTTCTATATTTTCCAATATGTCTTCATTGGATGATGAGGTTTCAGTTATGCCAATGATTCGCTTTTCTGAATGGTTGGATGAGTTCTTCTGTCGCTTATTTGCCTTACTTCTACACTTGGAACCAAGCAGTGTTACGAATGAAGGCTTGCACTCATCAGCAACATCAGGAACTTTTCTGGTTGAGGATGGTCCTTACTACTATTGCATGCTTGAAATCTTGTTTGGAAGACTCTCAAAACCCCTCTATAATCAGGCTTTGAAGAAGATATCTAAGTTTGTCAAGACAAATATCCTTCCGGGGGCAATTGCAGAGGTCGGACTGCTTTGTTGTGCATGTGTTTATTCAAACCCAGAAGAAGCAGTTACTCAACTGGTTGAGCCAATTTTACTGTCTGTTATATCCTCTTTAGAAGGAACACCGTCTACAGGATTTGGAGGTAGAGGAATATGCGATGCTTCAGTTTCAACCAAGGCTAAACCGACAATTTCTCCGGCTCTTGAAACAGCAATTGATTATCAATTAAAAATATTATCAGTTGCCGTAAGTTATGGAGGCCCTGCTCTTCTGCGATACAAGGATCAGTTCAAAGAGGCTGTTGTTTCTGCTTTTGAATCTCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGGTCTCTCCTTGGAAGCTTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTATTTTGCATCACCCAAATGCTACTGCATTAGAGGAATGGATCAGCTCAAAAGATTATTCAGACGATAAACCATTGGTGGGCCCTAAATGGCATATCCCTAGTGTTGAGGAAGTTAAATTTGCAAATGAACTTTTGGACCTTCATTTCCGGTCAGCTTTGGATGATCTTCTGAGAATATGTCAAACTAAAGTACATTCTGATCCAGGAGATGAGAAGGAGCACTTAAAAGTGACTCTTTTGCGAATAGATTCTTCATTGCAAGGTGTTTTGACTTGCTTGCCCGACTTCACCCCATCATCAAGGAATGGAACTGTTGAAAATCCAAATCATGGTTCCTTTTTAATAGCGGGAGCAACAGGTGCAACTGTGGGCAACACTAAATTGCGGGAAAAAGCTGCTGAGATTATACATGCAGCCTGCAAGTATTTACTTGAGAAAAAATCAGATGACAGCATCCTCTTGGTCCTCATCATTCGTATTATGGATGCTTTAGGAAATTATGGAAGTATGGAGTATGATGAATGGGCCAATCACAGGCAGGCATGGAAATTGGAATCTGCTGCCATTATAGAGCCTTCAATAAATTTTATTGTTTCAGCTCACGCTAAAGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCATTCATGCACAATACTTGGAGATCATCCCAGTCATCCTACCATATATTTCGCACTGGGAATTTTGGTCCACCAGAACATGTGAATCTTTTGGTGGATGATCTTCTAAATCTCTCTTTGCATAGCTATGAAACTGTTCGAATACTTGCTGGGAAATATCTTTTAAAAATGATAAAGAGATGGCCATCTATGATTTCAAAGTGTGTTCTCTCTCACACTGAGAACTTACGAAACCCCAAGGCACCAGAATATGCAGTGCTTGGGTCTTGTGCAGTCCTTGCTACACAAACAGTTCTCAAGCATTTGACAATGGACCCTAAGTCATTCTCTTCATTTATCCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAAACCCAGAAAGCTATCAACGAGCTTTTTGTCAAATACAACATTCACTTTGCTGGAGTATCGAGAAGCTATTTCAAGTCATCGGACAATCATGTTGATGCACCAGATTTTGCAGATTTGGTTTCTCAGATCAGTTCTATGAGTTTTGATTCTATGGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTTTATTGCTGTTGGCTATGGCATCTAGGAATGACCCAAGTTCCTCCTCCAAAATTCTGAGTGAAACTGCTGGTCACTTCCTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTAGCAATCTCAGCTCTAAATACACTATTAAAAGAATCACCTTATAAACTGTCAGATGAAAAACGGAGTTTTTCCTCAGAGAATTTGCAAGGAGCTAGAAAATCGTCTCTTGAAGGAGAGTTAACCCAAATATTTCAGGAAGATGGATTTTTCAGTGAGACTTTGAATAGTCTTTCTCATGTTCATATAATATCTGATACTGAAAGCAGTTCTAGAGGCAATCATGGGAACTCTTCTTTTCAGAGCCTAGCAGACAAATCAATCACCCGCTTTTATTTTGACTTTACCTCTTCATGGCCACGCACTCCAACTTGGATCTCTTTATTAGGAAGTGACACCTTTTACTCAAATTATGCGAGAATATTCAAACGGCTAATACAAGAATGTGGTTTGCCCGTCGTAGTGGCACTGAAAAGTTCATTGGAGGAGTTCTCAAATGCCAAGGAGAGGTCCAAGCAGTGCGTTGCTGCTGAAGCTTTCGCTGGAATATTGCATTCTGATGTTAATGGTATCAAAGAAGCATGGGATAATTGGATGGTGGTCCAGTTGCAGAACATAATTCTAGCGCATTCAGTAGAATCAATACCCGAATGGACAGCCTGCATACGTTATGCAGTTACTGGGAAAGGGAAATATGGAACTAGAGTTCCTCTTTTGAGACAACCAGTACTAGATTGTCTGGCCACACCTTTACCAGGAACAGTCACTACCACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCAGCCGCCCTTGTAGAACTATCTCCCCAGAAAATGCCATTAGCTGAAATTCAGCTCCACTATAGACTTCTGGAGGAGTTGCTTGGTAACATGTGCCATTCATCCGCCCAAGTAAGAGAAGCTATAGGTGTTGCCCTTTCTGTGTTGTGCTCTAACATTCGTCTTTATGAGTCATTTGATCATGATGGTTCAAATGGGAGTGCTAGTAAAGGTCTTGGCAACCAATTTGATGGGAGAAGTTGGGCTCTGTTTTTAAAAGAGCGAGCGTCGGAAGTGGTAATGAATATTCAGAATACCACTCATTCTGACAGTATGGAAACCCCAGAAAATATTACTCCTGAAAACGGGCATTTGAATGGTGATTCACAAGCTGATGTCAAATGGATGGAAACGCTTTTCCATTTTATCATCTCATCGTTGAGGTCTGCGAGATCATCATATCTGGTGGATGTAATTGTGGGATTTCTTTATCCTGTGATATCATTGCAGGAAACTTCAAGTAAAGAACTGTCAACCTTGGCCAAGGCAGCTTTTGAGTTGCTAAAATGGAGGATTTTTTGGGGACCTCATCTCCAAGAGGCCGTATCTGTGATTCTTTCTTCTGCCAATGACTCTAACTGGCGCACTAGATCGGCAACATTGACATATCTGCGGACTTTTATGTACAGGCACACTTTCATTCTCTCAATTGGGGAGAAACAACAAATTTGGAGAACTGTTGAAAAGTTACTTGTAGACAACCAAGTTGAGGTAAGAGAACATGCTGCAGCTGTTTTGGCGGGCCTAACGAAGGGTGGCGATGAAGATCTAGCTAAAGACTTCCGTGACAAAGCTTACAAAGAAGCTACTGATCTTCAAAGGAAGAGAAAACGGAGAAATTTGAGTTCAAGCCAACCCATAGCTTCCATTCATGGTGCTGTACTTGCTTTAGTTGCTTCCGTTCTGTCAGCGCCTTATGATATGCCCAGTTGGTTGCCAGATCATGTCACTTTACTTGCTCGTTTTGCTGGAGAACCAGCACCAGTGAAATCTACGGTTACAAAAGCAGTTGCAGAATTTCGACGAACCCATGCTGATACATGGAACATTCAGAAAGACTCATTTACCGAAGAGCAACTCGAGGTCCTGGCTGATACTTCTTCTTCATCTTCATATTTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBP

Protein Analysis

1814

Amino Acids

203.85

Weight (kDa)

7.03

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_PSME4_mid PF16507 310 - 506 6e-29 Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid PF16507 518 - 825 1.4e-19 Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C PF11919 1729 - 1814 6.1e-28 Proteasome activator complex subunit 4-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000538)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04050 FvH4_2g04050 FvH4_2g04050 FvH4_2g04050 FvH4_2g04050
rosa_chinensis RchiOBHm_Chr6g0253231 RchiOBHm_Chr6g0253251 RchiOBHm_Chr6g0253261 RchiOBHm_Chr6g0253271 RchiOBHm_Chr6g0253291 RchiOBHm_Chr6g0253321 RchiOBHm_Chr6g0253331 RchiOBHm_Chr6g0253341 RchiOBHm_Chr6g0253371 RchiOBHm_Chr6g0253401
rosa_laevigata RLG00000015022 RLG00000015023 RLG00000015024 RLG00000015027 RLG00000015028 RLG00000015030 RLG00000015031 RLG00000015034 RLG00000015035 RLG00000015038 RLG00000015041
rosa_multiflora Rmu_sc0001257.1_g000004 Rmu_sc0001257.1_g000008 Rmu_sc0001257.1_g000014 Rmu_sc0001257.1_g000016 Rmu_sc0001257.1_g000019 Rmu_sc0001257.1_g000026 Rmu_sc0002560.1_g000013 Rmu_sc0002560.1_g000020 Rmu_sc0002560.1_g000021 Rmu_sc0003855.1_g000005 Rmu_sc0003855.1_g000011 Rmu_sc0003898.1_g000003 Rmu_sc0003898.1_g000005 Rmu_sc0003898.1_g000016 Rmu_sc0003898.1_g000017 Rmu_sc0005439.1_g000007 Rmu_sc0005439.1_g000014 Rmu_sc0005439.1_g000021
rosa_roxburghii Rroxscaffold_7G00211930 Rroxscaffold_7G00211940 Rroxscaffold_7G00211960 Rroxscaffold_7G00211980 Rroxscaffold_7G00211990 Rroxscaffold_7G00212000 Rroxscaffold_7G00212050 Rroxscaffold_7G00212060 Rroxscaffold_7G00212070 Rroxscaffold_7G00212080 Rroxscaffold_7G00212160
rosa_rugosa Rorug05G0542900 Rorug05G0542900 Rorug05G0543000 Rorug05G0543100 Rorug05G0543100 Rorug05G0543200 Rorug05G0543300 Rorug05G0543400 Rorug05G0543400 Rorug05G0543500 Rorug05G0543600 Rorug05G0543700
rosa_samantha Rh6CG050000 Rh6DG045200 Rh6DG045500 Rh6DG045600 Rh6DG045700 Rh6DG045800 Rh6DG046200 Rh6DG046300 Rh6DG046400 Rh6DG046500 Rh6DG046700 Rh6DG046800
rosa_wichuraiana Rw0G000070 Rw6G005230 Rw6G005240 Rw6G005280 Rw6G005290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3585
AccB1I GGYRCC 2 cut(s) 336, 3094
AccB7I CCANNNNNTGG 1 cut(s) 2537
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 2 cut(s) 1922, 5016
AccII CGCG 3 cut(s) 75, 1146, 2833
AclWI GGATC 8 cut(s) 550, 617, 799, 1457, 2085, 2255, 2402, 3863
AcoI YGGCCR 5 cut(s) 976, 3035, 3836, 4221, 4800
AcuI CTGAAG 4 cut(s) 411, 1151, 1941, 4032
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 6 cut(s) 2201, 2400, 3401, 4209, 4946, 5202
AfeI AGCGCT 2 cut(s) 1037, 4268
AflIII ACRYGT 2 cut(s) 2932, 4371
AhdI GACNNNNNGTC 1 cut(s) 3172
AjnI CCWGG 6 cut(s) 466, 585, 901, 2410, 4234, 5406
AjuI GAANNNNNNNTTGG 6 cut(s) 1704, 1736, 2524, 2556, 3944, 3976
AloI GAACNNNNNNTCC 6 cut(s) 2160, 2192, 4170, 4171, 4202, 4203
Alw21I GWGCWC 3 cut(s) 924, 2430, 4433
Alw26I GTCTC 9 cut(s) 85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
AlwI GGATC 8 cut(s) 550, 617, 799, 1457, 2085, 2255, 2402, 3863
AlwNI CAGNNNCTG 5 cut(s) 389, 473, 1090, 1187, 4160
Ama87I CYCGRG 1 cut(s) 5399
Aor51HI AGCGCT 2 cut(s) 1037, 4268
ApaI GGGCCC 1 cut(s) 2291
ApeKI GCWGC 9 cut(s) 1187, 1190, 2603, 2621, 2768, 4007, 4808, 5045, 5048
AseI ATTAAT 1 cut(s) 1493
Asp700I GAANNNNTTC 3 cut(s) 2455, 2988, 3202
AspLEI GCGC 6 cut(s) 339, 1038, 4108, 4269, 4905, 5233
AsuC2I CCSGG 1 cut(s) 1797
AsuHPI GGTGA 8 cut(s) 1400, 1409, 2153, 2210, 2488, 3570, 3796, 4652
AsuII TTCGAA 1 cut(s) 2992
AvaI CYCGRG 1 cut(s) 5399
BaeGI GKGCMC 2 cut(s) 2291, 2842
BalI TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
BanI GGYRCC 2 cut(s) 336, 3094
BanII GRGCYC 2 cut(s) 2291, 4528
BauI CACGAG 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 1512, 1732
Bbv12I GWGCWC 3 cut(s) 924, 2430, 4433
BbvI GCAGC 9 cut(s) 1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BceAI ACGGC 5 cut(s) 428, 461, 2024, 3926, 4847
BciT130I CCWGG 6 cut(s) 468, 587, 903, 2412, 4236, 5408
BciVI GTATCC 2 cut(s) 807, 3531
BclI TGATCA 3 cut(s) 2143, 2194, 4459
BcnI CCSGG 1 cut(s) 1797
BcoDI GTCTC 9 cut(s) 85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BfmI CTRYAG 7 cut(s) 703, 1359, 1923, 4254, 4342, 4404, 5046
BfoI RGCGCY 4 cut(s) 340, 1039, 4270, 5234
BfuI GTATCC 2 cut(s) 807, 3531
BglII AGATCT 2 cut(s) 500, 5086
BmcAI AGTACT 1 cut(s) 4209
Bme1390I CCNGG 7 cut(s) 468, 587, 903, 1797, 2412, 4236, 5408
BmeRI GACNNNNNGTC 1 cut(s) 3172
BmeT110I CYCGRG 1 cut(s) 5399
BmiI GGNNCC 9 cut(s) 338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
BmrFI CCNGG 7 cut(s) 468, 587, 903, 1797, 2412, 4236, 5408
BmrI ACTGGG 4 cut(s) 2878, 2919, 4170, 5241
BmsI GCATC 9 cut(s) 13, 1253, 1444, 1942, 2224, 2670, 2683, 3316, 3452
BmuI ACTGGG 4 cut(s) 2878, 2919, 4170, 5241
BpiI GAAGAC 2 cut(s) 1512, 1732
BplI GAGNNNNNCTC 4 cut(s) 47, 79, 3956, 3988
BpmI CTGGAG 5 cut(s) 418, 938, 3303, 4373, 5310
Bpu14I TTCGAA 1 cut(s) 2992
BpuEI CTTGAG 2 cut(s) 2660, 3134
BpuMI CCSGG 1 cut(s) 1797
BsaAI YACGTR 1 cut(s) 1213
BsaBI GATNNNNATC 2 cut(s) 3037, 5085
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 2 cut(s) 151, 2163
BsaWI WCCGGW 1 cut(s) 2355
BsaXI ACNNNNNCTCC 6 cut(s) 1972, 2002, 4471, 4501, 4836, 4866
Bse3DI GCAATG 3 cut(s) 870, 2462, 4763
Bse8I GATNNNNATC 2 cut(s) 3037, 5085
BseBI CCWGG 6 cut(s) 468, 587, 903, 2412, 4236, 5408
BseJI GATNNNNATC 2 cut(s) 3037, 5085
BseMI GCAATG 3 cut(s) 870, 2462, 4763
BseRI GAGGAG 5 cut(s) 56, 73, 3457, 3980, 4370
BseSI GKGCMC 2 cut(s) 2291, 2842
BseX3I CGGCCG 1 cut(s) 976
BseXI GCAGC 9 cut(s) 1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BseYI CCCAGC 2 cut(s) 859, 3002
BsgI GTGCAG 1 cut(s) 3144
Bsh1236I CGCG 3 cut(s) 75, 1146, 2833
Bsh1285I CGRYCG 1 cut(s) 979
BshNI GGYRCC 2 cut(s) 336, 3094
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 3 cut(s) 924, 2430, 4433
BsiHKCI CYCGRG 1 cut(s) 5399
BsiSI CCGG 4 cut(s) 451, 1796, 1991, 2356
BslFI GGGAC 3 cut(s) 74, 647, 4857
BsmAI GTCTC 9 cut(s) 85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BsmBI CGTCTC 3 cut(s) 85, 1262, 3651
BsmFI GGGAC 3 cut(s) 74, 647, 4857
BsmI GAATGC 3 cut(s) 2855, 4033, 4108
Bso31I GGTCTC 2 cut(s) 151, 2163
BsoBI CYCGRG 1 cut(s) 5399
Bsp119I TTCGAA 1 cut(s) 2992
Bsp120I GGGCCC 1 cut(s) 2287
Bsp1286I GDGCHC 6 cut(s) 924, 2291, 2430, 2842, 4433, 4528
Bsp1407I TGTACA 1 cut(s) 4944
BspFNI CGCG 3 cut(s) 75, 1146, 2833
BspHI TCATGA 2 cut(s) 3217, 4462
BspLI GGNNCC 9 cut(s) 338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
BspMAI CTGCAG 1 cut(s) 5050
BspPI GGATC 8 cut(s) 550, 617, 799, 1457, 2085, 2255, 2402, 3863
BspQI GCTCTTC 2 cut(s) 2067, 5385
BspT104I TTCGAA 1 cut(s) 2992
BspT107I GGYRCC 2 cut(s) 336, 3094
BspTNI GGTCTC 2 cut(s) 151, 2163
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 3 cut(s) 870, 2462, 4763
BsrGI TGTACA 1 cut(s) 4944
BssNI GRCGYC 1 cut(s) 337
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 9 cut(s) 518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 6 cut(s) 468, 587, 903, 2412, 4236, 5408
Bst6I CTCTTC 8 cut(s) 1395, 2067, 2816, 3187, 3778, 3835, 5138, 5385
BstACI GRCGYC 1 cut(s) 337
BstAPI GCANNNNNTGC 2 cut(s) 2567, 3131
BstAUI TGTACA 1 cut(s) 4944
BstBAI YACGTR 1 cut(s) 1213
BstBI TTCGAA 1 cut(s) 2992
BstC8I GCNNGC 9 cut(s) 1646, 1706, 2486, 2626, 2748, 3389, 4141, 4545, 5062
BstDSI CCRYGG 1 cut(s) 2831
BstENI CCTNNNNNAGG 2 cut(s) 4233, 5069
BstFNI CGCG 3 cut(s) 75, 1146, 2833
BstH2I RGCGCY 4 cut(s) 340, 1039, 4270, 5234
BstHHI GCGC 6 cut(s) 339, 1038, 4108, 4269, 4905, 5233
BstMAI GTCTC 9 cut(s) 85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BstMCI CGRYCG 1 cut(s) 979
BstNI CCWGG 6 cut(s) 468, 587, 903, 2412, 4236, 5408
BstNSI RCATGY 6 cut(s) 1708, 1836, 2621, 2936, 4375, 5045
BstSCI CCNGG 7 cut(s) 466, 585, 901, 1795, 2410, 4234, 5406
BstSFI CTRYAG 7 cut(s) 703, 1359, 1923, 4254, 4342, 4404, 5046
BstSLI GKGCMC 2 cut(s) 2291, 2842
BstUI CGCG 3 cut(s) 75, 1146, 2833
BstV1I GCAGC 9 cut(s) 1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BstV2I GAAGAC 2 cut(s) 1512, 1732
BstX2I RGATCY 4 cut(s) 500, 542, 3855, 5086
BstXI CCANNNNNNTGG 1 cut(s) 4511
BstYI RGATCY 4 cut(s) 500, 542, 3855, 5086
BstZI CGGCCG 1 cut(s) 976
BsuI GTATCC 2 cut(s) 807, 3531
BtgI CCRYGG 1 cut(s) 2831
BtgZI GCGATG 2 cut(s) 1965, 5094
BtsI GCAGTG 3 cut(s) 1633, 3114, 4004
Cac8I GCNNGC 9 cut(s) 1646, 1706, 2486, 2626, 2748, 3389, 4141, 4545, 5062
CaiI CAGNNNCTG 5 cut(s) 389, 473, 1090, 1187, 4160
CciI TCATGA 2 cut(s) 3217, 4462
CciNI GCGGCCGC 1 cut(s) 976
CfoI GCGC 6 cut(s) 339, 1038, 4108, 4269, 4905, 5233
Cfr42I CCGCGG 1 cut(s) 2834
CseI GACGC 2 cut(s) 64, 4536
CsiI ACCWGGT 1 cut(s) 901
Csp6I GTAC 6 cut(s) 2200, 2399, 3400, 4208, 4945, 5201
CspCI CAANNNNNGTGG 2 cut(s) 4240, 4275
CviQI GTAC 6 cut(s) 2200, 2399, 3400, 4208, 4945, 5201
DinI GGCGCC 1 cut(s) 338
DraI TTTAAA 3 cut(s) 3018, 3513, 4536
DriI GACNNNNNGTC 1 cut(s) 3172
EaeI YGGCCR 5 cut(s) 976, 3035, 3836, 4221, 4800
EagI CGGCCG 1 cut(s) 976
Eam1104I CTCTTC 8 cut(s) 1395, 2067, 2816, 3187, 3778, 3835, 5138, 5385
Eam1105I GACNNNNNGTC 1 cut(s) 3172
EarI CTCTTC 8 cut(s) 1395, 2067, 2816, 3187, 3778, 3835, 5138, 5385
EciI GGCGGA 1 cut(s) 4375
EclXI CGGCCG 1 cut(s) 976
Eco130I CCWWGG 9 cut(s) 518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Eco24I GRGCYC 2 cut(s) 2291, 4528
Eco31I GGTCTC 2 cut(s) 151, 2163
Eco32I GATATC 2 cut(s) 1767, 4761
Eco47III AGCGCT 2 cut(s) 1037, 4268
Eco52I CGGCCG 1 cut(s) 976
Eco57I CTGAAG 4 cut(s) 411, 1151, 1941, 4032
Eco88I CYCGRG 1 cut(s) 5399
EcoNI CCTNNNNNAGG 2 cut(s) 4233, 5069
EcoO109I RGGNCCY 5 cut(s) 386, 2054, 2288, 4844, 5404
EcoRI GAATTC 2 cut(s) 590, 3198
EcoRII CCWGG 6 cut(s) 466, 585, 901, 2410, 4234, 5406
EcoRV GATATC 2 cut(s) 1767, 4761
EcoT14I CCWWGG 9 cut(s) 518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
EcoT22I ATGCAT 2 cut(s) 6, 1441
EcoT38I GRGCYC 2 cut(s) 2291, 4528
EgeI GGCGCC 1 cut(s) 338
EheI GGCGCC 1 cut(s) 338
ErhI CCWWGG 9 cut(s) 518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Esp3I CGTCTC 3 cut(s) 85, 1262, 3651
FalI AAGNNNNNCTT 4 cut(s) 2414, 2446, 2427, 2459
FaqI GGGAC 3 cut(s) 74, 647, 4857
FauI CCCGC 4 cut(s) 2548, 2586, 3815, 5053
FauNDI CATATG 1 cut(s) 1257
FbaI TGATCA 3 cut(s) 2143, 2194, 4459
FblI GTMKAC 2 cut(s) 1922, 5016
FriOI GRGCYC 2 cut(s) 2291, 4528
GlaI GCGC 6 cut(s) 338, 1037, 4107, 4268, 4904, 5232
GsaI CCCAGC 2 cut(s) 863, 3006
GsuI CTGGAG 5 cut(s) 418, 938, 3303, 4373, 5310
HaeII RGCGCY 4 cut(s) 340, 1039, 4270, 5234
HapII CCGG 4 cut(s) 451, 1796, 1991, 2356
HgaI GACGC 2 cut(s) 64, 4536
HhaI GCGC 6 cut(s) 339, 1038, 4108, 4269, 4905, 5233
Hin1I GRCGYC 1 cut(s) 337
Hin6I GCGC 6 cut(s) 337, 1036, 4106, 4267, 4903, 5231
HinP1I GCGC 6 cut(s) 337, 1036, 4106, 4267, 4903, 5231
HincII GTYRAC 4 cut(s) 940, 1353, 3663, 4794
HindII GTYRAC 4 cut(s) 940, 1353, 3663, 4794
HindIII AAGCTT 4 cut(s) 251, 2170, 4014, 5112
HpaI GTTAAC 1 cut(s) 3663
HpaII CCGG 4 cut(s) 451, 1796, 1991, 2356
HphI GGTGA 8 cut(s) 1400, 1409, 2153, 2210, 2488, 3570, 3796, 4652
Hpy166II GTNNAC 9 cut(s) 206, 940, 1331, 1353, 1923, 2924, 3663, 4794, 5017
Hpy8I GTNNAC 9 cut(s) 206, 940, 1331, 1353, 1923, 2924, 3663, 4794, 5017
Hpy99I CGWCG 4 cut(s) 80, 3941, 4552, 5346
HpyCH4IV ACGT 3 cut(s) 1212, 1327, 4147
HpySE526I ACGT 3 cut(s) 1212, 1327, 4147
Hsp92I GRCGYC 1 cut(s) 337
HspAI GCGC 6 cut(s) 337, 1036, 4106, 4267, 4903, 5231
KasI GGCGCC 1 cut(s) 336
Ksp22I TGATCA 3 cut(s) 2143, 2194, 4459
KspAI GTTAAC 1 cut(s) 3663
KspI CCGCGG 1 cut(s) 2834
LguI GCTCTTC 2 cut(s) 2067, 5385
LmnI GCTCC 9 cut(s) 110, 919, 1014, 1216, 2135, 2425, 2558, 3632, 4341
Lsp1109I GCAGC 9 cut(s) 1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
LweI GCATC 9 cut(s) 13, 1253, 1444, 1942, 2224, 2670, 2683, 3316, 3452
MabI ACCWGGT 1 cut(s) 901
MaeII ACGT 3 cut(s) 1212, 1327, 4147
MbiI CCGCTC 1 cut(s) 105
MfeI CAATTG 5 cut(s) 1052, 1371, 1803, 2007, 4968
MflI RGATCY 4 cut(s) 500, 542, 3855, 5086
MhlI GDGCHC 6 cut(s) 924, 2291, 2430, 2842, 4433, 4528
MlsI TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
MluNI TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
Mly113I GGCGCC 1 cut(s) 337
MlyI GAGTC 8 cut(s) 53, 56, 629, 1722, 2434, 4460, 4886, 5372
MmeI TCCRAC 6 cut(s) 489, 1303, 1554, 1795, 3872, 4530
Mox20I TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
Mph1103I ATGCAT 2 cut(s) 6, 1441
MroXI GAANNNNTTC 3 cut(s) 2455, 2988, 3202
MscI TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
MslI CAYNNNNRTG 5 cut(s) 2223, 2979, 3324, 4038, 4635
Msp20I TGGCCA 4 cut(s) 3037, 3838, 4223, 4802
MspA1I CMGCKG 3 cut(s) 981, 2833, 5051
MspI CCGG 4 cut(s) 451, 1796, 1991, 2356
MspR9I CCNGG 7 cut(s) 468, 587, 903, 1797, 2412, 4236, 5408
MunI CAATTG 5 cut(s) 1052, 1371, 1803, 2007, 4968
Mva1269I GAATGC 3 cut(s) 2855, 4033, 4108
MvaI CCWGG 6 cut(s) 468, 587, 903, 2412, 4236, 5408
MvnI CGCG 3 cut(s) 75, 1146, 2833
NarI GGCGCC 1 cut(s) 337
NciI CCSGG 1 cut(s) 1797
NdeI CATATG 1 cut(s) 1257
NlaIV GGNNCC 9 cut(s) 338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 6 cut(s) 2437, 3494, 3872, 4243, 5105, 5266
NotI GCGGCCGC 1 cut(s) 976
NsiI ATGCAT 2 cut(s) 6, 1441
NspI RCATGY 6 cut(s) 1708, 1836, 2621, 2936, 4375, 5045
NspV TTCGAA 1 cut(s) 2992
PaeI GCATGC 1 cut(s) 1708
PaeR7I CTCGAG 1 cut(s) 5399
PagI TCATGA 2 cut(s) 3217, 4462
PciI ACATGT 2 cut(s) 2932, 4371
PciSI GCTCTTC 2 cut(s) 2067, 5385
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 3 cut(s) 2855, 4033, 4108
PdmI GAANNNNTTC 3 cut(s) 2455, 2988, 3202
PflMI CCANNNNNTGG 1 cut(s) 2537
PfoI TCCNGGA 1 cut(s) 2410
PleI GAGTC 8 cut(s) 52, 56, 629, 1722, 2434, 4459, 4886, 5372
PluTI GGCGCC 1 cut(s) 340
PpsI GAGTC 8 cut(s) 52, 56, 629, 1722, 2434, 4459, 4886, 5372
Ppu21I YACGTR 1 cut(s) 1213
PpuMI RGGWCCY 3 cut(s) 386, 4844, 5404
PscI ACATGT 2 cut(s) 2932, 4371
PshBI ATTAAT 1 cut(s) 1493
PsiI TTATAA 1 cut(s) 3585
Psp5II RGGWCCY 3 cut(s) 386, 4844, 5404
Psp6I CCWGG 6 cut(s) 466, 585, 901, 2410, 4234, 5406
PspFI CCCAGC 2 cut(s) 859, 3002
PspGI CCWGG 6 cut(s) 466, 585, 901, 2410, 4234, 5406
PspN4I GGNNCC 9 cut(s) 338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
PspOMI GGGCCC 1 cut(s) 2287
PspPPI RGGWCCY 3 cut(s) 386, 4844, 5404
PspXI VCTCGAGB 1 cut(s) 5399
PsrI GAACNNNNNNTAC 4 cut(s) 1906, 1938, 4231, 4263
PstI CTGCAG 1 cut(s) 5050
PstNI CAGNNNCTG 5 cut(s) 389, 473, 1090, 1187, 4160
PsuI RGATCY 4 cut(s) 500, 542, 3855, 5086
PvuII CAGCTG 1 cut(s) 5051
RsaI GTAC 6 cut(s) 2201, 2400, 3401, 4209, 4946, 5202
RsaNI GTAC 6 cut(s) 2200, 2399, 3400, 4208, 4945, 5201
RseI CAYNNNNRTG 5 cut(s) 2223, 2979, 3324, 4038, 4635
SacII CCGCGG 1 cut(s) 2834
SapI GCTCTTC 2 cut(s) 2067, 5385
ScaI AGTACT 1 cut(s) 4209
SchI GAGTC 8 cut(s) 53, 56, 629, 1722, 2434, 4460, 4886, 5372
ScrFI CCNGG 7 cut(s) 468, 587, 903, 1797, 2412, 4236, 5408
SduI GDGCHC 6 cut(s) 924, 2291, 2430, 2842, 4433, 4528
SexAI ACCWGGT 1 cut(s) 901
SfaNI GCATC 9 cut(s) 13, 1253, 1444, 1942, 2224, 2670, 2683, 3316, 3452
SfcI CTRYAG 7 cut(s) 703, 1359, 1923, 4254, 4342, 4404, 5046
SfoI GGCGCC 1 cut(s) 338
Sfr274I CTCGAG 1 cut(s) 5399
Sfr303I CCGCGG 1 cut(s) 2834
SfuI TTCGAA 1 cut(s) 2992
SgrBI CCGCGG 1 cut(s) 2834
SlaI CTCGAG 1 cut(s) 5399
SmiMI CAYNNNNRTG 5 cut(s) 2223, 2979, 3324, 4038, 4635
SmlI CTYRAG 3 cut(s) 2639, 3149, 5399
SmoI CTYRAG 3 cut(s) 2639, 3149, 5399
SphI GCATGC 1 cut(s) 1708
SspDI GGCGCC 1 cut(s) 336
SspI AATATT 8 cut(s) 280, 956, 2028, 3672, 3903, 4029, 4567, 4612
StyD4I CCNGG 7 cut(s) 466, 585, 901, 1795, 2410, 4234, 5406
StyI CCWWGG 9 cut(s) 518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
TaiI ACGT 3 cut(s) 1215, 1330, 4150
TaqI TCGA 6 cut(s) 638, 1341, 2992, 3290, 5341, 5400
TaqII GACCGA 2 cut(s) 432, 2145
TatI WGTACW 5 cut(s) 2199, 2398, 4207, 4944, 5200
TauI GCSGC 4 cut(s) 978, 981, 2833, 4289
TseFI GTSAC 6 cut(s) 2437, 3494, 3872, 4243, 5105, 5266
TseI GCWGC 9 cut(s) 1187, 1190, 2603, 2621, 2768, 4007, 4808, 5045, 5048
Tsp45I GTSAC 6 cut(s) 2437, 3494, 3872, 4243, 5105, 5266
TspGWI ACGGA 5 cut(s) 76, 3619, 5093, 5167, 5209
Van91I CCANNNNNTGG 1 cut(s) 2537
VspI ATTAAT 1 cut(s) 1493
XagI CCTNNNNNAGG 2 cut(s) 4233, 5069
XbaI TCTAGA 1 cut(s) 3752
XceI RCATGY 6 cut(s) 1708, 1836, 2621, 2936, 4375, 5045
XcmI CCANNNNNNNNNTGG 1 cut(s) 968
XhoI CTCGAG 1 cut(s) 5399
XmiI GTMKAC 2 cut(s) 1922, 5016
XmnI GAANNNNTTC 3 cut(s) 2455, 2988, 3202
ZrmI AGTACT 1 cut(s) 4209
Zsp2I ATGCAT 2 cut(s) 6, 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.