AT4G04940

Transducin family protein WD-40 repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001399
Physical Location & Seq
Reverse (-)
2511028 .. 2517217
6190 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04940.2

Sequence Viewer

Length: 2733 bp
ATGGGAATTTTCGAACCGTTTAGAGCAATAGGTTACATAACGAGTACGGTTCCGTTCTCCGTCCAGCGATTGGGTACCGAGACTTTCGTCACCGTCAGCGTCGGCAAAGCTTTCCAGATTTATAATTGTGCCAAGCTCAATCTAGTCATAATCAGTCCTCAGCTTCCAAAGAAAATTAGAGCTTTAGCTTCTTACCGAGACTACACATTTGTTGCGTTTGGCAATGAAATCGCTGTCTTTAGGCGTGCTCATCAGGTAGCAACTTGGAGCAAACATGTTGCTAAAGTTGATCTTCTACTGGTGTTTGGAGAACATGTGCTTAGTCTTGACGTGGAAGGAAATATGTTCATATGGGCATTCAAAGGAATCGAAGAACATCTAGCTCCAATTGGAAATCTTCAGCTTACTGGAAAGTTTACCCCTACCTCTATTGTTCACCCAGATACCTATCTCAACAAGGTTCTTGTAGGGAGCCAAGAGGGTCCGCTGCAGCTATGGAATATTAATACTAAGAAGATGCTCTATCAGTTTAAGGGTTGGGGTTCATCTGTCACCAGTTGTGTTTCATCCCCTGCCCTGGATGTTGTTGCAATTGGTTGTGCTGATGGAAAGATCCATGTACATAACATAAAATTGGACGAAGAGATCGTAACATTTGAACATGCCTCGCGAGGTGCTGTTACTGCTTTGTCTTTCAGTACAGATGGACGTCCCCTTCTTGCGTCTGGAGGTTCCTTTGGTGTCATAAGCATCTGGAATCTTAACAAAAAAAGGCTTCAGTCAGTCATTAGGGATGCACATGACAGTTCTATAATCTCATTGAACTTTTTAGCCAATGAGCCGGTGCTAATGAGTGCATCAGCAGATAACTCACTTAAAATGTGGATTTTTGACACAAATGATGGTGATCCTCGCCTATTACGCTTCCGATCTGGGCATAGTGCTCCTCCTCTATGTATCAGGTTCTACTCTAATGGACGGCACATTTTGTCTGCTGGCCAGGATCGTGCCTTCCGCCTGTTCTCTGTCATCCAGGAGCAACAAAGTAGAGAGCTTTCTCAAAGACACATTTCTAGGCGAGCCAAAAAACTCCGACTGAAGGAGGAAGAGTTGAAATTGAAGCCTGTTGTTTCATTTGATTGCGCTGAAATTAGGGAGCGTGATTGGTGCAATGTTGTTACTTGCCATATGGATACAGCAGAAGCATATGTATGGAGACTTCAGAACTTTGTACTTGGAGAGCATATCCTCAAACCATGCCCTGAGAATCCAACACCAATTAAGGCATGTGCAATCAGTGCCTGTGGAAACTTTGCAGTAGTCGGAACAGCTGGTGGATGGATTGAGAGATTCAACCTCCAATCTGGAATCAGCCGGGGTAGTTACTTCGATATGTCAGAGAAAAGAAGGTACGCCCATGATGGAGAAGTTATTGGAGTTGCTTGCGACTCCACAAACACACTCATGATTAGTGCAGGATATCATGGGGACTTAAAGGTTTGGGATTTCAAAAAGCGGGAATTAAAGTCCCAGTGGGATGTCGGATGTTCGTTGGTTAAAATTGTCTACCACCGTGTAAATGGTCTTTTGGCTACAGTGGCGGATGATTTTGTTATCCGTTTATATGATGTTGTGACACTAAAGATGGTTCGTGAATTTAGAGGTCATACAGACCGTATAACGGATTTGTGCTTTAGTGAGGATGGCAAATGGGTCATCTCATCTAGCATGGATGGAAGTCTTAGAATATGGGATGTCATATTGGCTAAGCAGATTGATGGTGTGCATGTCGATGTGCCAATAACAGCATTGTCTTTATCACCAAATATGGACGTTTTGGCAACTGCCCACTCTGATCAGAACGGGGTCTACTTGTGGGTTAACCAATCCATGTTCTCTGGGCTTCCAAGTGTTGAATCTTATGCTAGTGGGAAAGATGTCGTAAATGTCAAGTTGCCTTCAGTCTCTGCATTGACATCTTCTGAAGCTGATGATGATATGGACAGGCAAGTGTTAGAAAATTCTGAAGCTCTACAAGCTAGCAGTTTCTCAATTTCCCAAAAGCAGATCCCAGAACTTGTTACTCTGTCTCTATTGCCAAAAAGCCAATGGCAGAGTTTGATCAACTTGGATATTATTAAGGCCCGCAATAAACCAATAGAGCCTCCAAAGAAACCTGAAAAGGCTCCTTTTTTCTTGCCCTCAATTCCCTCGCTGTCTGGAGATATATTATTCAAGGCAAATGACTCTGAGGCTGATGGGGAGAATGAGGAAAATAATAAAAAGGACCAGAATAGCATGAAGAACTTTGATGCATTGGAATCTCCATTCTCAAAACATCTCAAGTCTTCATGGGATTCAAAACACTTTTTAGATTTTACCAACTACATGAAGAGTTTATCCCCGTCAGCTCTCGACATGGAGCTTCGGATGCTTGAAATCATAGATGAAGATGTTGAAGAAGAGCTTATCAAAAGACCTGAGTTTATATTGATTGGGCAACTTCTGGATTACTTCATCAACGAGGTCTCTTGCAAAAACGACTTTGAGTTTATGCAGGCGGTTGTGAAACTATTTCTGAAGATCCACGGTGAGACCATAAGGTGCCACCCGAGTTTACAAGAGAAGGCGAAGAAGCTTTTAGAAACTCAGAGTTTGGTGTGGCAGAAAATGGAGAAGCTTTTCCAGAGCACAAGATGCATAGTTACCTTCCTTAGCAATTCACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

910

Amino Acids

102.22

Weight (kDa)

6.49

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 18 - 184 6.1e-70 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 30 - 235 2.4e-11 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 32 - 184 3.6e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 33 - 176 1.7e-08 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 38 - 181 5e-14 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 38 - 181 2.3e-16 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 39 - 143 5e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 40 - 146 9.6e-10 WDR90/POC16, second beta-propeller
Beta-prop_HPS5 PF23756 40 - 173 1.4e-06 HPS5 beta-propeller
Beta-prop_WDR3_1st PF25173 41 - 186 9.5e-22 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 41 - 184 7.6e-17 WDR5 beta-propeller domain
WDR90_beta-prop_4th PF23342 42 - 181 9.6e-07 WDR90, 4th beta-propeller
Beta-prop_EML PF23409 44 - 183 7.1e-12 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 62 - 229 1.7e-06 Aladin seven-bladed propeller
WDR55 PF24796 70 - 181 2.1e-09 WDR55
Beta-prop_WDR5 PF25175 109 - 240 8.2e-15 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 111 - 236 2.5e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 113 - 233 4.2e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 125 - 242 1.7e-07 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 125 - 232 9.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 205 - 521 4.6e-104 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 314 - 512 1.8e-18 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 316 - 502 3.2e-17 THOC3 beta-propeller domain
WD40_Prp19 PF24814 355 - 506 1.4e-15 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 359 - 507 3.1e-19 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 364 - 512 6.5e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 364 - 471 4.1e-08 MABP1/WDR62 second WD40 domain
WDR55 PF24796 365 - 472 3.9e-07 WDR55
WD40_CDC20-Fz PF24807 372 - 470 1.4e-09 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 374 - 475 1.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 404 - 471 6.7e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 406 - 500 7.3e-07 WDR90/POC16, second beta-propeller
EIF3I PF24805 407 - 498 4.6e-06 EIF3I
WD40_WDHD1_1st PF24817 407 - 475 1.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 424 - 495 1.4e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 434 - 471 5.4e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 435 - 507 1e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 575 - 792 1e-54 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 123
AatII GACGTC 1 cut(s) 712
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 2 cut(s) 74, 2604
AccB7I CCANNNNNTGG 1 cut(s) 70
AccI GTMKAC 2 cut(s) 1566, 1869
AccII CGCG 1 cut(s) 670
AciI CCGC 6 cut(s) 485, 1015, 1516, 1601, 2146, 2561
AclWI GGATC 5 cut(s) 607, 902, 1011, 2062, 2578
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 3 cut(s) 6, 1655, 2020
AcuI CTGAAG 8 cut(s) 383, 761, 1118, 1205, 1944, 2004, 2046, 2600
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 6 cut(s) 46, 76, 621, 700, 1233, 1412
AfiI CCNNNNNNNGG 4 cut(s) 70, 577, 1099, 1681
AflIII ACRYGT 2 cut(s) 274, 313
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 3 cut(s) 576, 999, 1032
Alw21I GWGCWC 3 cut(s) 250, 946, 2693
Alw26I GTCTC 7 cut(s) 74, 192, 1210, 1969, 2094, 2533, 2588
AlwI GGATC 5 cut(s) 607, 902, 1011, 2062, 2578
AlwNI CAGNNNCTG 2 cut(s) 1302, 1967
Ama87I CYCGRG 1 cut(s) 2611
AoxI GGCC 2 cut(s) 997, 2142
ApeKI GCWGC 2 cut(s) 487, 490
ApoI RAATTY 3 cut(s) 6, 1655, 2020
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AseI ATTAAT 1 cut(s) 504
Asp700I GAANNNNTTC 1 cut(s) 2681
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 1145
AspS9I GGNCC 3 cut(s) 482, 2143, 2287
AsuC2I CCSGG 1 cut(s) 1376
AsuHPI GGTGA 6 cut(s) 82, 428, 544, 917, 1812, 2603
AsuII TTCGAA 1 cut(s) 12
AsuNHI GCTAGC 1 cut(s) 2039
AvaI CYCGRG 1 cut(s) 2611
AvaII GGWCC 2 cut(s) 482, 2287
BalI TGGCCA 1 cut(s) 999
BanI GGYRCC 2 cut(s) 74, 2604
BarI GAAGNNNNNNTAC 2 cut(s) 1203, 1235
BbsI GAAGAC 1 cut(s) 2340
Bbv12I GWGCWC 3 cut(s) 250, 946, 2693
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 474, 502
BceAI ACGGC 1 cut(s) 995
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 3 cut(s) 578, 1001, 1034
BciVI GTATCC 1 cut(s) 1186
BclI TGATCA 2 cut(s) 1855, 2121
BcnI CCSGG 1 cut(s) 1376
BcoDI GTCTC 7 cut(s) 74, 192, 1210, 1969, 2094, 2533, 2588
BfaI CTAG 6 cut(s) 143, 380, 1074, 1725, 1926, 2040
BfmI CTRYAG 2 cut(s) 488, 1593
BfuI GTATCC 1 cut(s) 1186
BisI GCNGC 2 cut(s) 488, 491
BlpI GCTNAGC 1 cut(s) 1767
BlsI GCNGC 2 cut(s) 489, 492
Bme1390I CCNGG 4 cut(s) 578, 1001, 1034, 1376
Bme18I GGWCC 2 cut(s) 482, 2287
BmeT110I CYCGRG 1 cut(s) 2611
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 482, 2143, 2287
BmiI GGNNCC 7 cut(s) 51, 76, 473, 483, 733, 2187, 2606
BmrFI CCNGG 4 cut(s) 578, 1001, 1034, 1376
BmrI ACTGGG 1 cut(s) 1525
BmsI GCATC 7 cut(s) 507, 759, 784, 866, 2302, 2421, 2687
BmtI GCTAGC 1 cut(s) 2043
BmuI ACTGGG 1 cut(s) 1525
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 2340
BpmI CTGGAG 2 cut(s) 747, 2241
Bpu10I CCTNAGC 2 cut(s) 159, 2714
Bpu1102I GCTNAGC 1 cut(s) 1767
Bpu14I TTCGAA 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 2327
BpuMI CCSGG 1 cut(s) 1376
BsaBI GATNNNNATC 2 cut(s) 447, 906
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 2 cut(s) 2533, 2588
BsaJI CCNNGG 3 cut(s) 576, 1375, 2587
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 1094, 1124
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 577, 1099, 1681
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 4 cut(s) 303, 412, 555, 1531
Bse3DI GCAATG 2 cut(s) 229, 1177
Bse8I GATNNNNATC 2 cut(s) 447, 906
BseBI CCWGG 3 cut(s) 578, 1001, 1034
BseDI CCNNGG 3 cut(s) 576, 1375, 2587
BseJI GATNNNNATC 2 cut(s) 447, 906
BseLI CCNNNNNNNGG 4 cut(s) 70, 577, 1099, 1681
BseMI GCAATG 2 cut(s) 229, 1177
BseMII CTCAG 5 cut(s) 173, 1254, 2241, 2472, 2663
BseNI ACTGG 4 cut(s) 303, 412, 555, 1531
BseRI GAGGAG 2 cut(s) 936, 939
BseXI GCAGC 2 cut(s) 474, 502
BsgI GTGCAG 1 cut(s) 1494
Bsh1236I CGCG 1 cut(s) 670
BshFI GGCC 2 cut(s) 999, 2144
BshNI GGYRCC 2 cut(s) 74, 2604
BsiHKAI GWGCWC 3 cut(s) 250, 946, 2693
BsiHKCI CYCGRG 1 cut(s) 2611
BsiSI CCGG 2 cut(s) 842, 1375
BslFI GGGAC 3 cut(s) 696, 1502, 1513
BslI CCNNNNNNNGG 4 cut(s) 70, 577, 1099, 1681
BsmAI GTCTC 7 cut(s) 74, 192, 1210, 1969, 2094, 2533, 2588
BsmFI GGGAC 3 cut(s) 696, 1502, 1513
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 2 cut(s) 999, 2144
Bso31I GGTCTC 2 cut(s) 2533, 2588
BsoBI CYCGRG 1 cut(s) 2611
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 250, 946, 2693
Bsp1407I TGTACA 1 cut(s) 619
Bsp1720I GCTNAGC 1 cut(s) 1767
Bsp68I TCGCGA 1 cut(s) 670
BspACI CCGC 6 cut(s) 485, 1015, 1516, 1601, 2146, 2561
BspANI GGCC 2 cut(s) 999, 2144
BspCNI CTCAG 5 cut(s) 172, 1255, 2242, 2473, 2662
BspFNI CGCG 1 cut(s) 670
BspHI TCATGA 1 cut(s) 1464
BspLI GGNNCC 7 cut(s) 51, 76, 473, 483, 733, 2187, 2606
BspMAI CTGCAG 1 cut(s) 492
BspOI GCTAGC 1 cut(s) 2043
BspPI GGATC 5 cut(s) 607, 902, 1011, 2062, 2578
BspQI GCTCTTC 1 cut(s) 2457
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 74, 2604
BspTNI GGTCTC 2 cut(s) 2533, 2588
BsrDI GCAATG 2 cut(s) 229, 1177
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 4 cut(s) 303, 412, 555, 1531
BssAI RCCGGY 1 cut(s) 841
BssECI CCNNGG 3 cut(s) 576, 1375, 2587
BssNI GRCGYC 1 cut(s) 709
Bst2UI CCWGG 3 cut(s) 578, 1001, 1034
Bst4CI ACNGT 9 cut(s) 18, 49, 94, 806, 1574, 1597, 1676, 2591, 2727
Bst6I CTCTTC 4 cut(s) 636, 1101, 2387, 2457
BstACI GRCGYC 1 cut(s) 709
BstAPI GCANNNNNTGC 2 cut(s) 1298, 2697
BstAUI TGTACA 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 7 cut(s) 246, 997, 1080, 1444, 2041, 2146, 2559
BstDSI CCRYGG 1 cut(s) 2587
BstFNI CGCG 1 cut(s) 670
BstHHI GCGC 1 cut(s) 1145
BstMAI GTCTC 7 cut(s) 74, 192, 1210, 1969, 2094, 2533, 2588
BstMWI GCNNNNNNNGC 7 cut(s) 683, 921, 1298, 1598, 2036, 2431, 2697
BstNI CCWGG 3 cut(s) 578, 1001, 1034
BstNSI RCATGY 5 cut(s) 278, 317, 665, 1290, 1790
BstPAI GACNNNNGTC 1 cut(s) 86
BstSCI CCNGG 4 cut(s) 576, 999, 1032, 1374
BstSFI CTRYAG 2 cut(s) 488, 1593
BstUI CGCG 1 cut(s) 670
BstV1I GCAGC 2 cut(s) 474, 502
BstV2I GAAGAC 1 cut(s) 2340
BstX2I RGATCY 3 cut(s) 612, 2067, 2583
BstYI RGATCY 3 cut(s) 612, 2067, 2583
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 2 cut(s) 999, 2144
BtgI CCRYGG 1 cut(s) 2587
BtrI CACGTC 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 1303, 1538, 1602
BtuMI TCGCGA 1 cut(s) 670
Cac8I GCNNGC 7 cut(s) 246, 997, 1080, 1444, 2041, 2146, 2559
CaiI CAGNNNCTG 2 cut(s) 1302, 1967
CciI TCATGA 1 cut(s) 1464
CfoI GCGC 1 cut(s) 1145
Cfr10I RCCGGY 1 cut(s) 841
Cfr13I GGNCC 3 cut(s) 482, 2143, 2287
CseI GACGC 2 cut(s) 88, 711
Csp6I GTAC 6 cut(s) 45, 75, 620, 699, 1232, 1411
CviQI GTAC 6 cut(s) 45, 75, 620, 699, 1232, 1411
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 4 cut(s) 636, 1101, 2387, 2457
EarI CTCTTC 4 cut(s) 636, 1101, 2387, 2457
EciI GGCGGA 2 cut(s) 1004, 1616
Eco31I GGTCTC 2 cut(s) 2533, 2588
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 2 cut(s) 482, 2287
Eco57I CTGAAG 8 cut(s) 383, 761, 1118, 1205, 1944, 2004, 2046, 2600
Eco88I CYCGRG 1 cut(s) 2611
EcoRII CCWGG 3 cut(s) 576, 999, 1032
EcoRV GATATC 1 cut(s) 1481
EcoT22I ATGCAT 2 cut(s) 2317, 2702
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 2451, 2483
FaqI GGGAC 3 cut(s) 696, 1502, 1513
FauI CCCGC 2 cut(s) 1509, 2153
FauNDI CATATG 3 cut(s) 350, 1188, 1207
FbaI TGATCA 2 cut(s) 1855, 2121
FblI GTMKAC 2 cut(s) 1566, 1869
Fnu4HI GCNGC 2 cut(s) 488, 491
Fsp4HI GCNGC 2 cut(s) 488, 491
FspBI CTAG 6 cut(s) 143, 380, 1074, 1725, 1926, 2040
GlaI GCGC 1 cut(s) 1144
GluI GCNGC 2 cut(s) 488, 491
GsuI CTGGAG 2 cut(s) 747, 2241
HaeIII GGCC 2 cut(s) 999, 2144
HapII CCGG 2 cut(s) 842, 1375
HgaI GACGC 2 cut(s) 88, 711
HhaI GCGC 1 cut(s) 1145
Hin1I GRCGYC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 1143
HinP1I GCGC 1 cut(s) 1143
HincII GTYRAC 1 cut(s) 1882
HindII GTYRAC 1 cut(s) 1882
HindIII AAGCTT 3 cut(s) 108, 2636, 2678
HpaI GTTAAC 1 cut(s) 1882
HpaII CCGG 2 cut(s) 842, 1375
HphI GGTGA 6 cut(s) 82, 428, 544, 917, 1812, 2603
Hpy166II GTNNAC 6 cut(s) 417, 436, 1567, 1870, 1882, 2618
Hpy8I GTNNAC 6 cut(s) 417, 436, 1567, 1870, 1882, 2618
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 8 cut(s) 329, 725, 1021, 1093, 1401, 1968, 2620, 2719
HpyCH4III ACNGT 9 cut(s) 18, 49, 94, 806, 1574, 1597, 1676, 2591, 2727
HpyCH4IV ACGT 3 cut(s) 330, 709, 1833
HpyF10VI GCNNNNNNNGC 7 cut(s) 683, 921, 1298, 1598, 2036, 2431, 2697
HpySE526I ACGT 3 cut(s) 330, 709, 1833
Hsp92I GRCGYC 1 cut(s) 709
HspAI GCGC 1 cut(s) 1143
KpnI GGTACC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 1855, 2121
KspAI GTTAAC 1 cut(s) 1882
LguI GCTCTTC 1 cut(s) 2457
LmnI GCTCC 8 cut(s) 267, 388, 471, 949, 1036, 1156, 2191, 2422
Lsp1109I GCAGC 2 cut(s) 474, 502
LweI GCATC 7 cut(s) 507, 759, 784, 866, 2302, 2421, 2687
MaeI CTAG 6 cut(s) 143, 380, 1074, 1725, 1926, 2040
MaeII ACGT 3 cut(s) 330, 709, 1833
MfeI CAATTG 2 cut(s) 387, 591
MflI RGATCY 3 cut(s) 612, 2067, 2583
MhlI GDGCHC 3 cut(s) 250, 946, 2693
MlsI TGGCCA 1 cut(s) 999
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 2 cut(s) 1442, 2240
MmeI TCCRAC 4 cut(s) 1117, 1295, 1303, 1522
Mox20I TGGCCA 1 cut(s) 999
Mph1103I ATGCAT 2 cut(s) 2317, 2702
MroXI GAANNNNTTC 1 cut(s) 2681
MscI TGGCCA 1 cut(s) 999
MslI CAYNNNNRTG 3 cut(s) 1210, 1463, 1791
Msp20I TGGCCA 1 cut(s) 999
MspA1I CMGCKG 2 cut(s) 487, 1331
MspI CCGG 2 cut(s) 842, 1375
MspR9I CCNGG 4 cut(s) 578, 1001, 1034, 1376
MunI CAATTG 2 cut(s) 387, 591
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 3 cut(s) 578, 1001, 1034
MvnI CGCG 1 cut(s) 670
MwoI GCNNNNNNNGC 7 cut(s) 683, 921, 1298, 1598, 2036, 2431, 2697
NciI CCSGG 1 cut(s) 1376
NdeI CATATG 3 cut(s) 350, 1188, 1207
NheI GCTAGC 1 cut(s) 2039
NlaIV GGNNCC 7 cut(s) 51, 76, 473, 483, 733, 2187, 2606
NmuCI GTSAC 3 cut(s) 88, 550, 1633
NruI TCGCGA 1 cut(s) 670
NsiI ATGCAT 2 cut(s) 2317, 2702
NspI RCATGY 5 cut(s) 278, 317, 665, 1290, 1790
NspV TTCGAA 1 cut(s) 12
PagI TCATGA 1 cut(s) 1464
PciI ACATGT 2 cut(s) 274, 313
PciSI GCTCTTC 1 cut(s) 2457
PcsI WCGNNNNNNNCGW 1 cut(s) 645
PctI GAATGC 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 2681
PfeI GAWTC 8 cut(s) 366, 757, 1267, 1350, 1368, 1916, 2321, 2357
PflMI CCANNNNNTGG 1 cut(s) 70
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 2 cut(s) 489, 492
PleI GAGTC 2 cut(s) 1442, 2240
PpsI GAGTC 2 cut(s) 1442, 2240
PscI ACATGT 2 cut(s) 274, 313
PshAI GACNNNNGTC 1 cut(s) 86
PshBI ATTAAT 1 cut(s) 504
PsiI TTATAA 1 cut(s) 123
Psp6I CCWGG 3 cut(s) 576, 999, 1032
PspGI CCWGG 3 cut(s) 576, 999, 1032
PspN4I GGNNCC 7 cut(s) 51, 76, 473, 483, 733, 2187, 2606
PspPI GGNCC 3 cut(s) 482, 2143, 2287
PsrI GAACNNNNNNTAC 2 cut(s) 1853, 1885
PstI CTGCAG 1 cut(s) 492
PstNI CAGNNNCTG 2 cut(s) 1302, 1967
PsuI RGATCY 3 cut(s) 612, 2067, 2583
PvuII CAGCTG 1 cut(s) 1331
RruI TCGCGA 1 cut(s) 670
RsaI GTAC 6 cut(s) 46, 76, 621, 700, 1233, 1412
RsaNI GTAC 6 cut(s) 45, 75, 620, 699, 1232, 1411
RseI CAYNNNNRTG 3 cut(s) 1210, 1463, 1791
SapI GCTCTTC 1 cut(s) 2457
SatI GCNGC 2 cut(s) 488, 491
Sau96I GGNCC 3 cut(s) 482, 2143, 2287
SchI GAGTC 2 cut(s) 1442, 2240
ScrFI CCNGG 4 cut(s) 578, 1001, 1034, 1376
SduI GDGCHC 3 cut(s) 250, 946, 2693
SfaNI GCATC 7 cut(s) 507, 759, 784, 866, 2302, 2421, 2687
SfcI CTRYAG 2 cut(s) 488, 1593
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 2 cut(s) 482, 2287
SmiMI CAYNNNNRTG 3 cut(s) 1210, 1463, 1791
SmlI CTYRAG 1 cut(s) 2342
SmoI CTYRAG 1 cut(s) 2342
SsiI CCGC 6 cut(s) 485, 1015, 1516, 1601, 2146, 2561
SspI AATATT 1 cut(s) 502
SspMI CTAG 6 cut(s) 143, 380, 1074, 1725, 1926, 2040
StyD4I CCNGG 4 cut(s) 576, 999, 1032, 1374
TaaI ACNGT 9 cut(s) 18, 49, 94, 806, 1574, 1597, 1676, 2591, 2727
TaiI ACGT 3 cut(s) 333, 712, 1836
TaqI TCGA 5 cut(s) 12, 369, 1389, 1791, 2415
TatI WGTACW 3 cut(s) 619, 698, 1231
TfiI GAWTC 8 cut(s) 366, 757, 1267, 1350, 1368, 1916, 2321, 2357
TscAI CASTG 3 cut(s) 1303, 1538, 1602
TseFI GTSAC 3 cut(s) 88, 550, 1633
TseI GCWGC 2 cut(s) 487, 490
Tsp45I GTSAC 3 cut(s) 88, 550, 1633
TspGWI ACGGA 4 cut(s) 42, 49, 1607, 1697
TspRI CASTG 3 cut(s) 1303, 1538, 1602
Van91I CCANNNNNTGG 1 cut(s) 70
VpaK11BI GGWCC 2 cut(s) 482, 2287
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 3 cut(s) 6, 1655, 2020
XceI RCATGY 5 cut(s) 278, 317, 665, 1290, 1790
XcmI CCANNNNNNNNNTGG 2 cut(s) 1577, 2106
XmiI GTMKAC 2 cut(s) 1566, 1869
XmnI GAANNNNTTC 1 cut(s) 2681
XspI CTAG 6 cut(s) 143, 380, 1074, 1725, 1926, 2040
ZraI GACGTC 1 cut(s) 710
Zsp2I ATGCAT 2 cut(s) 2317, 2702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.