Rroxscaffold_5G00342190

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001399
Physical Location & Seq
Reverse (-)
10975541 .. 10984192
8652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342190.1

Sequence Viewer

Length: 2442 bp
ATGGGAATCTTCGAACCCTTCAGAGCAATCGGCTACACCACCACCTCCGTCCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTCGTCGGTCCGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGCACCGGGACTACACATTCGCTGCGTATGGGACTGATATAGCTGTTTTAAAGCGATCTCATCAGGTTGCTACGTGGAGCGCCCATAATGCGAAGGTCAATTTGTTGCTATTGTTTGGAGAGCATATCCTAAGTGTTGATGTTGAAGGTAATCTGTATATATGGGCATTTAAAGGAATTGATGAGAATCTTGATCCGGTTGGACATATTAAGTTGGACGAAAATTTTACTCCTAGCTGCATAATGCATCCGGATACCTACTTAAATAAGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCACCACAAAGAAAAAACTGTATGAGTTCAAGGGATGGAAATCTTCTATATGCAGCTGTGTTTCTTCTCCTGCGCTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATTTGATGAAGAGTTAGTGACGTTTACTCATTCAACACGAGGGGCTGTGACTGCCTTATCTTTCAGCACAGGTAGGATCATAGTAGATATGTTGGTGATTATGTGGATTTTTGACACAAGCGATGGAGATGCTCGTCTTTTACGGTTTAGAAGTGGTCATAGTGCTCCTCCACAATGCATAAGGTTTTATGCAAATGGGAGACACATTCTATCGGCTGGTCAGGATCGTGCCTTTCGCCTTTTCTCTATAATCCAGGATCAACAAAGTAGAGAGCTTTCTCAACGTCACATAACAAAACGAGCGAAGAAACTCAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTTGTTACATGTCATATGGATACTGCACAGGCATATGTTTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCGATCAGTGCATGTGGAAATTTTGCCATACTAGGGACAGCTGGTGGTTGGATTGAGCGGTTCAACCTGCAGTCAGGAAGCAGCCGAGGTAATTATGTGGACCCATCGGAAAGAAGCATCTGTGCCCATGATGGGGAAGTTGTTGGAATTGCTTGTGATTCAACTAATACTCACATGATAAGTGCCGGATATCATGGAGACATAAAGGTGTGGGATTTCAAGGGACGTGGAGTGCCATCTAGATGGGAAGTTGGGAGCTCTGTTGTCAGGATTGTATTCAATCGTTTGAATGGTCTCTTGGCTGTTGCGGCTGATGACTTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACTGATCGTGTTACAGACATGTGTTTTAGCGAGGATGGAAAATGGCTTTTGTCTTCCAGTATGGATGGAAGTCTTCGAGTTTGGGATGTTATCTTAGCAAGACAAATAGATGCGTTGCATGTTGATGTGTCTGTGACGGCATTGTCTCTATCACCAAATATGGATATTTTAGCGACCTCCCATGTTGATCAAAATGGGGTCTATCTTTGGGTGAATCAATCAATGTTTTCAGGAGCTTCAAAGGTAGATTCCTATGCAAGTGGAAAGCGAGTTGTGAGTGTTAAGTTGCCATCTGTCTCTTCGGCAAAAGGTTCTGAAGCTGAGGATTCTGACGAGCCTGTCGGAAACTCTGCACAACTTAAAGATGCTTCTGCTCTTCCAACTCTTGTACAGCAAATTCCAGATCTTGTTACTCTCTCATCTTATCCTAAAAGCCTGTGGCAAAGCTTAATCAACTTAGACATTATAAAGGAACGAAATAAACCGATTGAGCCCCCAAAGAAACCCGAAAAGGCTCCTTTCTTTTTGCCATCAGTTCCATCTCTTTCAGGAGAGATAATGTTTAAGCCTACTGGGTCGACAAATGAGGAGGCAAAAGTTGGTGAAGAATCATCTAGAATAAAGGCTGCTCTAGCACCATCAATATTCCTTCAAGCGCTACAGTCATCTGCAGAAATGAACAATTTTTCAGAATTTACTGATTTCATCAAATCTTTGTCTCCATCATCCCTGGATATGGAACTTCGAATTCTTCAGATTGTGGGTGATGAGGATGAAGAAGAGCCAGAAAAGTTGCTTCCAATTGAACTGCTTCTCGACTATTTTATACACGAGACTGCATGCAGAAACAATTTTGACTTTGTACAAGCTGTGATCAGGGTTTTTCTGAAGGTTGGCCGAGATGATGCTCTCTCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

813

Amino Acids

90.2

Weight (kDa)

6.26

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 27 - 233 2.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 30 - 290 1.7e-70 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 82 - 233 1.1e-06 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 103 - 299 7e-10 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 112 - 235 1.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 152 - 300 5.1e-08 Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 153 - 234 1.1e-07 WDR90/POC16, second beta-propeller
Beta-prop_TEP1_2nd PF25047 154 - 222 5.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 155 - 304 1.3e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 155 - 307 1.1e-12 WDR5 beta-propeller domain
WD40_Prp19 PF24814 256 - 529 1.8e-12 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 270 - 587 3.7e-103 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 378 - 577 1.5e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 384 - 566 3.2e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 419 - 568 1.3e-14 Prp19 WD40 domain
WDR55 PF24796 423 - 537 2.2e-07 WDR55
WD40_MABP1-WDR62_2nd PF24782 432 - 536 6.7e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 433 - 569 1.7e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st PF25173 467 - 572 1.6e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 467 - 582 6.9e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 468 - 548 4.7e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 469 - 543 2.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 479 - 578 6.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR75_1st PF23869 484 - 560 8e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 500 - 536 4e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 501 - 572 4.5e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 639 - 808 1.4e-41 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1988
AasI GACNNNNNNGTC 2 cut(s) 1663, 1859
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1218
AccBSI CCGCTC 1 cut(s) 1201
AccI GTMKAC 1 cut(s) 2099
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 4 cut(s) 158, 1201, 1451, 1471
AclWI GGATC 4 cut(s) 377, 716, 864, 897
AcoI YGGCCR 1 cut(s) 2416
AcsI RAATTY 8 cut(s) 117, 412, 612, 1162, 1508, 1917, 2213, 2268
AcuI CTGAAG 5 cut(s) 4, 99, 1857, 2258, 2429
AfaI GTAC 2 cut(s) 1911, 2385
AfeI AGCGCT 1 cut(s) 2178
AfiI CCNNNNNNNGG 4 cut(s) 1176, 1275, 1276, 2257
AflIII ACRYGT 2 cut(s) 1034, 1539
AjiI CACGTC 1 cut(s) 1370
AjnI CCWGG 2 cut(s) 885, 2250
AjuI GAANNNNNNNTTGG 2 cut(s) 1424, 1456
Alw21I GWGCWC 3 cut(s) 184, 799, 1403
Alw26I GTCTC 8 cut(s) 826, 1063, 1335, 1442, 1671, 1822, 2244, 2348
AlwI GGATC 4 cut(s) 377, 716, 864, 897
Aor13HI TCCGGA 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 2178
AoxI GGCC 3 cut(s) 1137, 1516, 2416
ApeKI GCWGC 6 cut(s) 212, 426, 490, 555, 1224, 2147
ApoI RAATTY 8 cut(s) 117, 412, 612, 1162, 1508, 1917, 2213, 2268
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 2331
AspLEI GCGC 5 cut(s) 69, 131, 272, 577, 2179
AspS9I GGNCC 3 cut(s) 145, 155, 1243
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 8 cut(s) 82, 496, 739, 961, 1665, 1744, 2135, 2297
AsuII TTCGAA 2 cut(s) 12, 2266
AvaII GGWCC 3 cut(s) 145, 155, 1243
BaeGI GKGCMC 1 cut(s) 1270
BanII GRGCYC 3 cut(s) 184, 1403, 2016
BauI CACGAG 2 cut(s) 669, 2351
BbsI GAAGAC 2 cut(s) 1566, 1586
Bbv12I GWGCWC 3 cut(s) 184, 799, 1403
BbvCI CCTCAGC 1 cut(s) 1842
BbvI GCAGC 6 cut(s) 199, 413, 502, 567, 1236, 2134
BceAI ACGGC 1 cut(s) 1674
BcgI CGANNNNNNTGC 4 cut(s) 743, 777, 1002, 1036
BciT130I CCWGG 2 cut(s) 887, 2252
BciVI GTATCC 2 cut(s) 436, 1039
BclI TGATCA 2 cut(s) 1708, 2394
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 8 cut(s) 826, 1063, 1335, 1442, 1671, 1822, 2244, 2348
BfaI CTAG 6 cut(s) 423, 578, 1175, 1383, 2136, 2153
BfmI CTRYAG 4 cut(s) 488, 1211, 2180, 2190
BfoI RGCGCY 3 cut(s) 70, 273, 2180
BfuAI ACCTGC 1 cut(s) 1218
BfuI GTATCC 2 cut(s) 436, 1039
BglII AGATCT 1 cut(s) 1924
BisI GCNGC 7 cut(s) 213, 427, 491, 556, 1225, 1452, 2148
BlsI GCNGC 7 cut(s) 214, 428, 492, 557, 1226, 1453, 2149
Bme1390I CCNGG 3 cut(s) 197, 887, 2252
Bme18I GGWCC 3 cut(s) 145, 155, 1243
BmgBI CACGTC 1 cut(s) 1370
BmgT120I GGNCC 3 cut(s) 145, 155, 1243
BmiI GGNNCC 2 cut(s) 1245, 2037
BmrFI CCNGG 3 cut(s) 197, 887, 2252
BmrI ACTGGG 1 cut(s) 2103
BmsI GCATC 7 cut(s) 445, 751, 1269, 1499, 1621, 1876, 2416
BmuI ACTGGG 1 cut(s) 2103
BpiI GAAGAC 2 cut(s) 1566, 1586
Bpu10I CCTNAGC 1 cut(s) 1842
Bpu14I TTCGAA 2 cut(s) 12, 2266
BpuMI CCSGG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 264
BsaBI GATNNNNATC 2 cut(s) 375, 541
BsaI GGTCTC 1 cut(s) 1442
BsaJI CCNNGG 3 cut(s) 70, 1228, 2250
BsaWI WCCGGW 2 cut(s) 385, 439
Bsc4I CCNNNNNNNGG 4 cut(s) 1176, 1275, 1276, 2257
Bse1I ACTGG 2 cut(s) 1578, 2098
Bse3DI GCAATG 2 cut(s) 981, 1030
Bse8I GATNNNNATC 2 cut(s) 375, 541
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 2 cut(s) 887, 2252
BseDI CCNNGG 3 cut(s) 70, 1228, 2250
BseGI GGATG 7 cut(s) 436, 542, 1561, 1591, 1612, 2246, 2299
BseJI GATNNNNATC 2 cut(s) 375, 541
BseLI CCNNNNNNNGG 4 cut(s) 1176, 1275, 1276, 2257
BseMI GCAATG 2 cut(s) 981, 1030
BseMII CTCAG 2 cut(s) 958, 1833
BseNI ACTGG 2 cut(s) 1578, 2098
BseRI GAGGAG 2 cut(s) 789, 2123
BseSI GKGCMC 1 cut(s) 1270
BseXI GCAGC 6 cut(s) 199, 413, 502, 567, 1236, 2134
BsgI GTGCAG 2 cut(s) 1035, 1857
BshFI GGCC 3 cut(s) 1139, 1518, 2418
BsiHKAI GWGCWC 3 cut(s) 184, 799, 1403
BsiSI CCGG 4 cut(s) 196, 386, 440, 1329
BslFI GGGAC 5 cut(s) 35, 212, 235, 1192, 1380
BslI CCNNNNNNNGG 4 cut(s) 1176, 1275, 1276, 2257
BsmAI GTCTC 8 cut(s) 826, 1063, 1335, 1442, 1671, 1822, 2244, 2348
BsmFI GGGAC 5 cut(s) 35, 212, 235, 1192, 1380
BsnI GGCC 3 cut(s) 1139, 1518, 2418
Bso31I GGTCTC 1 cut(s) 1442
Bsp119I TTCGAA 2 cut(s) 12, 2266
Bsp1286I GDGCHC 5 cut(s) 184, 799, 1270, 1403, 2016
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 2 cut(s) 1909, 2383
BspACI CCGC 4 cut(s) 158, 1201, 1451, 1471
BspANI GGCC 3 cut(s) 1139, 1518, 2418
BspCNI CTCAG 2 cut(s) 957, 1834
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 1245, 2037
BspMAI CTGCAG 3 cut(s) 492, 1215, 2194
BspMI ACCTGC 1 cut(s) 1218
BspPI GGATC 4 cut(s) 377, 716, 864, 897
BspQI GCTCTTC 2 cut(s) 1902, 2295
BspT104I TTCGAA 2 cut(s) 12, 2266
BspTNI GGTCTC 1 cut(s) 1442
BsrBI CCGCTC 1 cut(s) 1201
BsrDI GCAATG 2 cut(s) 981, 1030
BsrGI TGTACA 2 cut(s) 1909, 2383
BsrI ACTGG 2 cut(s) 1578, 2098
BssECI CCNNGG 3 cut(s) 70, 1228, 2250
BssSI CACGAG 2 cut(s) 669, 2351
Bst2BI CACGAG 2 cut(s) 669, 2351
Bst2UI CCWGG 2 cut(s) 887, 2252
Bst4CI ACNGT 4 cut(s) 94, 522, 777, 2184
Bst6I CTCTTC 5 cut(s) 636, 954, 1825, 1902, 2295
BstAUI TGTACA 2 cut(s) 1909, 2383
BstBAI YACGTR 1 cut(s) 264
BstBI TTCGAA 2 cut(s) 12, 2266
BstC8I GCNNGC 1 cut(s) 2362
BstDEI CTNAG 5 cut(s) 320, 944, 1615, 1842, 1978
BstF5I GGATG 7 cut(s) 436, 542, 1561, 1591, 1612, 2246, 2299
BstH2I RGCGCY 3 cut(s) 70, 273, 2180
BstHHI GCGC 5 cut(s) 69, 131, 272, 577, 2179
BstMAI GTCTC 8 cut(s) 826, 1063, 1335, 1442, 1671, 1822, 2244, 2348
BstMWI GCNNNNNNNGC 5 cut(s) 278, 683, 1151, 1451, 2153
BstNI CCWGG 2 cut(s) 887, 2252
BstNSI RCATGY 5 cut(s) 1038, 1158, 1543, 1643, 2364
BstSCI CCNGG 3 cut(s) 195, 885, 2250
BstSFI CTRYAG 4 cut(s) 488, 1211, 2180, 2190
BstSLI GKGCMC 1 cut(s) 1270
BstV1I GCAGC 6 cut(s) 199, 413, 502, 567, 1236, 2134
BstV2I GAAGAC 2 cut(s) 1566, 1586
BstX2I RGATCY 1 cut(s) 1924
BstXI CCANNNNNNTGG 1 cut(s) 1386
BstYI RGATCY 1 cut(s) 1924
BsuI GTATCC 2 cut(s) 436, 1039
BsuRI GGCC 3 cut(s) 1139, 1518, 2418
BtgZI GCGATG 1 cut(s) 768
BtrI CACGTC 1 cut(s) 1370
BtsCI GGATG 7 cut(s) 436, 542, 1561, 1591, 1612, 2246, 2299
BtsIMutI CAGTG 2 cut(s) 75, 1156
BveI ACCTGC 1 cut(s) 1218
Cac8I GCNNGC 1 cut(s) 2362
CfoI GCGC 5 cut(s) 69, 131, 272, 577, 2179
Cfr13I GGNCC 3 cut(s) 145, 155, 1243
CpoI CGGWCCG 1 cut(s) 155
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 1910, 2384
CspCI CAANNNNNGTGG 2 cut(s) 1351, 1386
CspI CGGWCCG 1 cut(s) 155
CviQI GTAC 2 cut(s) 1910, 2384
DdeI CTNAG 5 cut(s) 320, 944, 1615, 1842, 1978
DraI TTTAAA 2 cut(s) 240, 361
DrdI GACNNNNNNGTC 2 cut(s) 1663, 1859
DseDI GACNNNNNNGTC 2 cut(s) 1663, 1859
EaeI YGGCCR 1 cut(s) 2416
Eam1104I CTCTTC 5 cut(s) 636, 954, 1825, 1902, 2295
EarI CTCTTC 5 cut(s) 636, 954, 1825, 1902, 2295
Ecl136II GAGCTC 2 cut(s) 182, 1401
Eco147I AGGCCT 1 cut(s) 1139
Eco24I GRGCYC 3 cut(s) 184, 1403, 2016
Eco31I GGTCTC 1 cut(s) 1442
Eco32I GATATC 1 cut(s) 1334
Eco47I GGWCC 3 cut(s) 145, 155, 1243
Eco47III AGCGCT 1 cut(s) 2178
Eco53kI GAGCTC 2 cut(s) 182, 1401
Eco57I CTGAAG 5 cut(s) 4, 99, 1857, 2258, 2429
EcoICRI GAGCTC 2 cut(s) 182, 1401
EcoRI GAATTC 1 cut(s) 2268
EcoRII CCWGG 2 cut(s) 885, 2250
EcoRV GATATC 1 cut(s) 1334
EcoT22I ATGCAT 2 cut(s) 438, 812
EcoT38I GRGCYC 3 cut(s) 184, 1403, 2016
FaqI GGGAC 5 cut(s) 35, 212, 235, 1192, 1380
FauNDI CATATG 2 cut(s) 1041, 1060
FbaI TGATCA 2 cut(s) 1708, 2394
FblI GTMKAC 1 cut(s) 2099
Fnu4HI GCNGC 7 cut(s) 213, 427, 491, 556, 1225, 1452, 2148
FokI GGATG 7 cut(s) 423, 549, 1568, 1598, 1619, 2233, 2306
FriOI GRGCYC 3 cut(s) 184, 1403, 2016
Fsp4HI GCNGC 7 cut(s) 213, 427, 491, 556, 1225, 1452, 2148
FspBI CTAG 6 cut(s) 423, 578, 1175, 1383, 2136, 2153
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 5 cut(s) 68, 130, 271, 576, 2178
GluI GCNGC 7 cut(s) 213, 427, 491, 556, 1225, 1452, 2148
HaeII RGCGCY 3 cut(s) 70, 273, 2180
HaeIII GGCC 3 cut(s) 1139, 1518, 2418
HapII CCGG 4 cut(s) 196, 386, 440, 1329
HgaI GACGC 1 cut(s) 88
HhaI GCGC 5 cut(s) 69, 131, 272, 577, 2179
Hin6I GCGC 5 cut(s) 67, 129, 270, 575, 2177
HinP1I GCGC 5 cut(s) 67, 129, 270, 575, 2177
HincII GTYRAC 1 cut(s) 2100
HindII GTYRAC 1 cut(s) 2100
HindIII AAGCTT 1 cut(s) 1966
HinfI GANTC 9 cut(s) 6, 175, 376, 472, 1301, 1735, 1769, 1847, 2129
HpaII CCGG 4 cut(s) 196, 386, 440, 1329
HphI GGTGA 8 cut(s) 82, 496, 739, 961, 1665, 1744, 2135, 2297
Hpy166II GTNNAC 4 cut(s) 622, 657, 1243, 2100
Hpy188I TCNGA 9 cut(s) 23, 1104, 1252, 1837, 1852, 1865, 2212, 2277, 2409
Hpy8I GTNNAC 4 cut(s) 622, 657, 1243, 2100
Hpy99I CGWCG 2 cut(s) 104, 155
HpyCH4III ACNGT 4 cut(s) 94, 522, 777, 2184
HpyCH4IV ACGT 4 cut(s) 263, 653, 916, 1369
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 683, 1151, 1451, 2153
HpyF3I CTNAG 5 cut(s) 320, 944, 1615, 1842, 1978
HpySE526I ACGT 4 cut(s) 263, 653, 916, 1369
HspAI GCGC 5 cut(s) 67, 129, 270, 575, 2177
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 2 cut(s) 1708, 2394
LguI GCTCTTC 2 cut(s) 1902, 2295
LmnI GCTCC 6 cut(s) 267, 802, 1093, 1398, 1754, 2041
Lsp1109I GCAGC 6 cut(s) 199, 413, 502, 567, 1236, 2134
LweI GCATC 7 cut(s) 445, 751, 1269, 1499, 1621, 1876, 2416
MaeI CTAG 6 cut(s) 423, 578, 1175, 1383, 2136, 2153
MaeII ACGT 4 cut(s) 263, 653, 916, 1369
MaeIII GTNAC 8 cut(s) 88, 649, 679, 917, 1030, 1531, 1654, 1930
MbiI CCGCTC 1 cut(s) 1201
MfeI CAATTG 3 cut(s) 124, 161, 2322
MflI RGATCY 1 cut(s) 1924
MhlI GDGCHC 5 cut(s) 184, 799, 1270, 1403, 2016
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 6 cut(s) 370, 384, 1172, 1267, 1843, 1925
Mph1103I ATGCAT 2 cut(s) 438, 812
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 2331
MseI TTAA 9 cut(s) 239, 360, 399, 452, 1463, 1803, 1881, 1970, 2085
MslI CAYNNNNRTG 3 cut(s) 1349, 1384, 1644
MspA1I CMGCKG 2 cut(s) 558, 1184
MspI CCGG 4 cut(s) 196, 386, 440, 1329
MspR9I CCNGG 3 cut(s) 197, 887, 2252
MunI CAATTG 3 cut(s) 124, 161, 2322
MvaI CCWGG 2 cut(s) 887, 2252
MwoI GCNNNNNNNGC 5 cut(s) 278, 683, 1151, 1451, 2153
NciI CCSGG 1 cut(s) 197
NdeI CATATG 2 cut(s) 1041, 1060
NlaIV GGNNCC 2 cut(s) 1245, 2037
NmeAIII GCCGAG 2 cut(s) 51, 1253
NmuCI GTSAC 5 cut(s) 88, 649, 679, 917, 1654
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 2 cut(s) 438, 812
NspI RCATGY 5 cut(s) 1038, 1158, 1543, 1643, 2364
NspV TTCGAA 2 cut(s) 12, 2266
PaeI GCATGC 1 cut(s) 2364
PceI AGGCCT 1 cut(s) 1139
PciI ACATGT 2 cut(s) 1034, 1539
PciSI GCTCTTC 2 cut(s) 1902, 2295
PdmI GAANNNNTTC 1 cut(s) 2331
PfeI GAWTC 8 cut(s) 6, 175, 376, 1301, 1735, 1769, 1847, 2129
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 7 cut(s) 214, 428, 492, 557, 1226, 1453, 2149
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 1 cut(s) 264
PscI ACATGT 2 cut(s) 1034, 1539
PsiI TTATAA 1 cut(s) 1988
Psp124BI GAGCTC 2 cut(s) 184, 1403
Psp6I CCWGG 2 cut(s) 885, 2250
PspGI CCWGG 2 cut(s) 885, 2250
PspN4I GGNNCC 2 cut(s) 1245, 2037
PspPI GGNCC 3 cut(s) 145, 155, 1243
PstI CTGCAG 3 cut(s) 492, 1215, 2194
PsuI RGATCY 1 cut(s) 1924
PvuII CAGCTG 2 cut(s) 558, 1184
RsaI GTAC 2 cut(s) 1911, 2385
RsaNI GTAC 2 cut(s) 1910, 2384
RseI CAYNNNNRTG 3 cut(s) 1349, 1384, 1644
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 184, 1403
SalI GTCGAC 1 cut(s) 2098
SapI GCTCTTC 2 cut(s) 1902, 2295
SaqAI TTAA 9 cut(s) 239, 360, 399, 452, 1463, 1803, 1881, 1970, 2085
SatI GCNGC 7 cut(s) 213, 427, 491, 556, 1225, 1452, 2148
Sau96I GGNCC 3 cut(s) 145, 155, 1243
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 197, 887, 2252
SduI GDGCHC 5 cut(s) 184, 799, 1270, 1403, 2016
SfaNI GCATC 7 cut(s) 445, 751, 1269, 1499, 1621, 1876, 2416
SfcI CTRYAG 4 cut(s) 488, 1211, 2180, 2190
SfuI TTCGAA 2 cut(s) 12, 2266
SinI GGWCC 3 cut(s) 145, 155, 1243
SmiMI CAYNNNNRTG 3 cut(s) 1349, 1384, 1644
SphI GCATGC 1 cut(s) 2364
SseBI AGGCCT 1 cut(s) 1139
SsiI CCGC 4 cut(s) 158, 1201, 1451, 1471
SspI AATATT 1 cut(s) 2166
SspMI CTAG 6 cut(s) 423, 578, 1175, 1383, 2136, 2153
SstI GAGCTC 2 cut(s) 184, 1403
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 3 cut(s) 195, 885, 2250
TaaI ACNGT 4 cut(s) 94, 522, 777, 2184
TaiI ACGT 4 cut(s) 266, 656, 919, 1372
TaqI TCGA 6 cut(s) 12, 178, 1597, 2099, 2266, 2337
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 2 cut(s) 1909, 2383
TauI GCSGC 1 cut(s) 1454
TfiI GAWTC 8 cut(s) 6, 175, 376, 1301, 1735, 1769, 1847, 2129
Tru1I TTAA 9 cut(s) 239, 360, 399, 452, 1463, 1803, 1881, 1970, 2085
Tru9I TTAA 9 cut(s) 239, 360, 399, 452, 1463, 1803, 1881, 1970, 2085
TscAI CASTG 2 cut(s) 82, 1156
TseFI GTSAC 5 cut(s) 88, 649, 679, 917, 1654
TseI GCWGC 6 cut(s) 212, 426, 490, 555, 1224, 2147
Tsp45I GTSAC 5 cut(s) 88, 649, 679, 917, 1654
TspDTI ATGAA 5 cut(s) 605, 654, 2213, 2215, 2310
TspGWI ACGGA 2 cut(s) 37, 49
TspRI CASTG 2 cut(s) 82, 1156
VpaK11BI GGWCC 3 cut(s) 145, 155, 1243
XapI RAATTY 8 cut(s) 117, 412, 612, 1162, 1508, 1917, 2213, 2268
XbaI TCTAGA 2 cut(s) 1382, 2135
XceI RCATGY 5 cut(s) 1038, 1158, 1543, 1643, 2364
XmiI GTMKAC 1 cut(s) 2099
XmnI GAANNNNTTC 1 cut(s) 2331
XspI CTAG 6 cut(s) 423, 578, 1175, 1383, 2136, 2153
Zsp2I ATGCAT 2 cut(s) 438, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.