FvH4_4g06010

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001399
Physical Location & Seq
Reverse (-)
5415476 .. 5424516
9041 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06010.t1

Sequence Viewer

Length: 2703 bp
ATGGGAATCTTCGAGCCGTTCAGAGCAATCGGGTACACAACCAGCTCCGTCCCCTTCTCCGTTCAGCGTCTCGGCACCGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCTTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTTGTTGGTCCGCAATTGCCCAAGAAGATTCGAGCTCTTGCTTCGTACCGTGACTATACATTTGTTGCATATGGGACCGACATTGCTGTTTTCAAGCGGTCTCATCAGGTAGCTACGTGGAGCAACCATAATGCGAAAGTCAATTTGTTGCTATTGTTTGGAGAGCACATCCTAAGTGCTGATATTGATGGTAATCTGTATATATGGGGATTTAAAGGAATTGATGAGAACCTTGAACCAGTTGGACATATTAAGTTGGATGAAAATTTTACTCCTAGCTGTATAATGCATCCGGATACCTACTTAAATAAGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCAGCACAAAGAAAAAACTGTATGAGTTCAAGGGATGGAAATCTTCGATATGCAGCTGTGTTTCGTCTCCTGCACTAGATGTTGTTGCAATTGGTTGTGCTGATGGAAAAGTTCATGTTCACAACATACGATATGATGAAGAGATAGTGACGTTTACTCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGCAGCCTCTTCTAGCATCTGGTGGTTCTTCTGGTGTCATAAGCATATGGAATCTCGAAAAAAGAAGGCTTCAGTCAGTTATAAAAGAGGCACATGATGGTTCTGTACTTTCACTGCACTTTTTTGTGAATGAACCAGTGTTATTGAGTTCATCATCAGACAATTCTATTAAAATGTGGATTTTTGACACAAGCGATGGAGTTGCTCGTCTTCTACGGTTTAGAAGTGGTCATAGTGCTCCTCCACAGTCCATAAGGTTTTACGCAAATGGGAGACACATTCTATCGGCTGGTCAAGATCGGGCCTTTCGCCTTTTCTCTATAATCCAGGATCATCAAAGTAGAGAGCTTTCTCAACGTCATATAACAAAAAGAGCGAAGAAACTGAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGACTGGTGCAATGTTGTTACATGTCACATGGATACTGCACAGGCATATGTGTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCAATCAGTGCGTGTGGAAATTTTGCCATTCTAGGGACAGCTGGTGGTTGGATTGAGCGGTTCAATCTGCAGTCAGGAAGCAGCCGAGGTAGTTATGTGGACCCATTGGAAAGAAGCGGCTCTGCCCATGAGGGGGAAGTTGTTGGAATTGCATGTGATTCAACTAATACTCACATGATAAGTGCTGGATATCATGGGGACATAAAGGTCTGGGATTTCAAGGGACGTGGAGTGCCATCTAGATGGGAAGTTGGGAGCTCTGTTGTCAGGATTGTATTCAATCGTTTGAATGGTCTTTTGGCTGTTGCCGCTGATGACCTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACTGATCGCATCACAGACATGTGTTTTAGTGAGGATGGAAAGTGGCTTTTGTCTTCCAGTATGGATGGAAGTCTTCGAGTTTGGGATGTTATCTTAGCAAGACAAATAGATGCGTTGCACGTAGATGTGTCTGTGACGGCATTGTCTCTGTCACCGAATATGGATATTTTAGCAACCTCCCATGTAGATCAAAACGGGGTCTATCTTTGGGTGAATCAATCAATGTTTTCCGGAGCATCGAAGGTAGGTTCCTATGCAAGTGGAAAGCAAGTTGTTAGTGTTAAGTTGCCATCTGTCTCTTCGACAAAAGGTTCTGAAGCTGAGGATATTGACGAGCCTGTTGGAAACTCTGCACAAATTGAAGATTCTCTTCCAACTCTTGTACTGCAAATTCCAGATCTTGTTACTCTCTCATCATATCCTAAAAGCCAGTGGCAAAGCTTAATCAACTTAGACGTTATAAAGGAACGAAATAAACCAATTGAGCCCCCAAAGAAACCCGAAAAGGCTCCTTTCTTTTTGCCTTCAGTTCCTTCTCTTTCAGGAGAAATAATGTTTAAGCCTACTGGGTCAACAAATGAGGAGGCAAAAGGTGGTGAAGAATCATCTAGAATAAAGGGTGCTCTAGCACCATCAGTATTCCTTCAAGTGCTTCAGTCATCTGCAGAAATGAAGAATTTTTCAGAATTTACTGATTTCATCAAAACTTTGTCCCCGTCATCCCTGGATATGGAACTTCGAATTCTTCAGATTGTTGGTGATGAGGATGAAGAAGAGCCAGAAAAGTTGGTTCCAATTGAACTGCTTCTAGACTATTTTATACACGAGACTGCATGCAGAAATAATTTTGACTTTGTGCAAGCTGTGATCAGAGTATTTCTGAAGATCCATGGCGAGACAATTCGACGCCAGTCACGACTACAAGATAAGGCAAGCAAGCTCCTAGACGTTCAACAAAACACATGGCAAAGAGTAGATAAAATGTTTCAAAGTGCCAGGTGCATGGTCACTTTTCTTAGCAATTCACAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

901

Amino Acids

100.06

Weight (kDa)

6.68

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 298 2.3e-100 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 84 - 274 8.8e-15 Prp19 WD40 domain
WD40_Gbeta PF25391 126 - 348 1e-15 G protein beta WD-40 repeat protein
Beta-prop_RIG_2nd PF23775 135 - 286 1.3e-06 RIG second beta-propeller
WD40_CDC20-Fz PF24807 142 - 300 1.5e-18 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 146 - 300 6.9e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 259 3.1e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 152 - 258 1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 153 - 260 2.5e-12 WDR90/POC16, second beta-propeller
Beta-prop_WDR5 PF25175 153 - 298 1.8e-17 WDR5 beta-propeller domain
Beta-prop_HPS5 PF23756 154 - 276 1.3e-07 HPS5 beta-propeller
Beta-prop_WDR3_1st PF25173 155 - 343 3.7e-26 WDR3 first beta-propeller domain
WDR90_beta-prop_4th PF23342 156 - 298 3.6e-07 WDR90, 4th beta-propeller
Beta-prop_WDR5 PF25175 156 - 355 6.2e-22 WDR5 beta-propeller domain
Beta-prop_EML PF23409 157 - 297 1.7e-14 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 175 - 343 3e-07 Aladin seven-bladed propeller
WDR55 PF24796 183 - 295 7.7e-10 WDR55
Beta-prop_VPS8 PF23410 194 - 291 9e-07 Vps8 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 222 - 360 1.5e-10 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 223 - 341 6e-07 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 225 - 302 2.1e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 225 - 302 3.9e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 227 - 342 2.2e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 239 - 356 1.4e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 636 6.5e-104 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 426 - 626 8e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 431 - 615 2.4e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 469 - 616 2.7e-14 Prp19 WD40 domain
WD40_Gbeta PF25391 472 - 607 9.8e-06 G protein beta WD-40 repeat protein
WDR55 PF24796 473 - 586 1.3e-07 WDR55
Beta-prop_WDR3_1st PF25173 475 - 621 6.1e-20 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 478 - 586 7.4e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 482 - 618 2e-08 WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 482 - 585 1.3e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 516 - 631 1.2e-08 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 517 - 596 6.7e-12 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 528 - 627 1e-05 WDR36/Utp21 first beta-propeller
WD40 PF00400 549 - 585 7.8e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 550 - 621 5.9e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 688 - 896 1.6e-54 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 788, 2129
AasI GACNNNNNNGTC 2 cut(s) 1496, 1810
Acc16I TGCGCA 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 1348
AccIII TCCGGA 2 cut(s) 439, 1898
AciI CCGC 6 cut(s) 158, 244, 1348, 1407, 1599, 1618
AclWI GGATC 2 cut(s) 1044, 2548
AcsI RAATTY 8 cut(s) 117, 412, 1309, 1655, 2058, 2344, 2354, 2409
AcuI CTGAAG 6 cut(s) 761, 2004, 2178, 2306, 2399, 2570
AcyI GRCGYC 1 cut(s) 2575
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 4 cut(s) 35, 194, 813, 2052
AfiI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2398
AflIII ACRYGT 2 cut(s) 1181, 1686
AjiI CACGTC 1 cut(s) 1517
AjnI CCWGG 3 cut(s) 1032, 2391, 2663
AjuI GAANNNNNNNTTGG 2 cut(s) 1571, 1603
Alw21I GWGCWC 5 cut(s) 184, 315, 946, 1550, 2293
AlwI GGATC 2 cut(s) 1044, 2548
Aor13HI TCCGGA 2 cut(s) 439, 1898
AoxI GGCC 3 cut(s) 1008, 1284, 1663
ApeKI GCWGC 4 cut(s) 490, 555, 709, 1371
ApoI RAATTY 8 cut(s) 117, 412, 1309, 1655, 2058, 2344, 2354, 2409
ArsI GACNNNNNNTTYG 4 cut(s) 72, 104, 191, 223
Asp700I GAANNNNTTC 1 cut(s) 2472
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 5 cut(s) 145, 155, 222, 1008, 1390
AsuHPI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2276, 2438
AsuII TTCGAA 1 cut(s) 2407
AvaII GGWCC 4 cut(s) 145, 155, 222, 1390
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 3 cut(s) 184, 1550, 2157
BauI CACGAG 2 cut(s) 669, 2492
BbsI GAAGAC 3 cut(s) 908, 1713, 1733
Bbv12I GWGCWC 5 cut(s) 184, 315, 946, 1550, 2293
BbvCI CCTCAGC 1 cut(s) 1989
BbvI GCAGC 4 cut(s) 502, 567, 721, 1383
BceAI ACGGC 1 cut(s) 1821
BcgI CGANNNNNNTGC 4 cut(s) 890, 924, 1149, 1183
BciT130I CCWGG 3 cut(s) 1034, 2393, 2665
BciVI GTATCC 2 cut(s) 436, 1186
BclI TGATCA 1 cut(s) 2535
BfaI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2277, 2294, 2477, 2612
BfmI CTRYAG 3 cut(s) 488, 1358, 2331
BfuI GTATCC 2 cut(s) 436, 1186
BglII AGATCT 1 cut(s) 2065
BisI GCNGC 6 cut(s) 491, 556, 710, 1372, 1408, 1599
BlsI GCNGC 6 cut(s) 492, 557, 711, 1373, 1409, 1600
Bme1390I CCNGG 3 cut(s) 1034, 2393, 2665
Bme18I GGWCC 4 cut(s) 145, 155, 222, 1390
BmgBI CACGTC 1 cut(s) 1517
BmgT120I GGNCC 5 cut(s) 145, 155, 222, 1008, 1390
BmiI GGNNCC 6 cut(s) 76, 223, 1392, 1918, 2178, 2460
BmrFI CCNGG 3 cut(s) 1034, 2393, 2665
BmrI ACTGGG 1 cut(s) 2244
BmsI GCATC 6 cut(s) 445, 731, 1646, 1686, 1768, 1913
BmuI ACTGGG 1 cut(s) 2244
BoxI GACNNNNGTC 1 cut(s) 2578
BpiI GAAGAC 3 cut(s) 908, 1713, 1733
Bpu10I CCTNAGC 1 cut(s) 1989
Bpu14I TTCGAA 1 cut(s) 2407
BsaAI YACGTR 2 cut(s) 264, 1789
BsaBI GATNNNNATC 2 cut(s) 339, 541
BsaHI GRCGYC 1 cut(s) 2575
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 1375, 2391, 2557
BsaWI WCCGGW 2 cut(s) 439, 1898
Bsc4I CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2398
Bse1I ACTGG 7 cut(s) 386, 842, 1169, 1725, 2098, 2239, 2578
Bse3DI GCAATG 3 cut(s) 228, 1128, 1177
Bse8I GATNNNNATC 2 cut(s) 339, 541
BseAI TCCGGA 2 cut(s) 439, 1898
BseBI CCWGG 3 cut(s) 1034, 2393, 2665
BseDI CCNNGG 3 cut(s) 1375, 2391, 2557
BseGI GGATG 9 cut(s) 315, 412, 436, 542, 1708, 1738, 1759, 2387, 2440
BseJI GATNNNNATC 2 cut(s) 339, 541
BseLI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2398
BseMI GCAATG 3 cut(s) 228, 1128, 1177
BseMII CTCAG 2 cut(s) 1082, 1980
BseNI ACTGG 7 cut(s) 386, 842, 1169, 1725, 2098, 2239, 2578
BseRI GAGGAG 2 cut(s) 936, 2264
BseXI GCAGC 4 cut(s) 502, 567, 721, 1383
BsgI GTGCAG 4 cut(s) 558, 806, 1182, 2004
BshFI GGCC 3 cut(s) 1010, 1286, 1665
BshNI GGYRCC 1 cut(s) 74
BsiHKAI GWGCWC 5 cut(s) 184, 315, 946, 1550, 2293
BsiSI CCGG 2 cut(s) 440, 1899
BslFI GGGAC 6 cut(s) 35, 235, 1339, 1502, 1527, 2365
BslI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2398
BsmBI CGTCTC 2 cut(s) 74, 573
BsmFI GGGAC 6 cut(s) 35, 235, 1339, 1502, 1527, 2365
BsnI GGCC 3 cut(s) 1010, 1286, 1665
Bso31I GGTCTC 1 cut(s) 252
Bsp119I TTCGAA 1 cut(s) 2407
Bsp1286I GDGCHC 6 cut(s) 184, 315, 946, 1550, 2157, 2293
Bsp13I TCCGGA 2 cut(s) 439, 1898
Bsp143I GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
Bsp19I CCATGG 1 cut(s) 2557
BspACI CCGC 6 cut(s) 158, 244, 1348, 1407, 1599, 1618
BspANI GGCC 3 cut(s) 1010, 1286, 1665
BspCNI CTCAG 2 cut(s) 1083, 1981
BspEI TCCGGA 2 cut(s) 439, 1898
BspLI GGNNCC 6 cut(s) 76, 223, 1392, 1918, 2178, 2460
BspMAI CTGCAG 3 cut(s) 492, 1362, 2335
BspPI GGATC 2 cut(s) 1044, 2548
BspQI GCTCTTC 1 cut(s) 2436
BspT104I TTCGAA 1 cut(s) 2407
BspT107I GGYRCC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 3 cut(s) 228, 1128, 1177
BsrI ACTGG 7 cut(s) 386, 842, 1169, 1725, 2098, 2239, 2578
BssECI CCNNGG 3 cut(s) 1375, 2391, 2557
BssMI GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
BssNI GRCGYC 1 cut(s) 2575
BssSI CACGAG 2 cut(s) 669, 2492
BssT1I CCWWGG 1 cut(s) 2557
Bst2BI CACGAG 2 cut(s) 669, 2492
Bst2UI CCWGG 3 cut(s) 1034, 2393, 2665
Bst4CI ACNGT 5 cut(s) 94, 197, 522, 924, 954
Bst6I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2043, 2436
BstACI GRCGYC 1 cut(s) 2575
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstBAI YACGTR 2 cut(s) 264, 1789
BstBI TTCGAA 1 cut(s) 2407
BstC8I GCNNGC 4 cut(s) 2503, 2529, 2602, 2606
BstDEI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2119, 2684
BstDSI CCRYGG 1 cut(s) 2557
BstF5I GGATG 9 cut(s) 315, 412, 436, 542, 1708, 1738, 1759, 2387, 2440
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 7 cut(s) 1006, 1039, 1675, 1858, 2068, 2538, 2556
BstMBI GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
BstMWI GCNNNNNNNGC 3 cut(s) 683, 1298, 1598
BstNI CCWGG 3 cut(s) 1034, 2393, 2665
BstNSI RCATGY 4 cut(s) 1185, 1446, 1690, 2505
BstPAI GACNNNNGTC 1 cut(s) 2578
BstSCI CCNGG 3 cut(s) 1032, 2391, 2663
BstSFI CTRYAG 3 cut(s) 488, 1358, 2331
BstV1I GCAGC 4 cut(s) 502, 567, 721, 1383
BstV2I GAAGAC 3 cut(s) 908, 1713, 1733
BstX2I RGATCY 2 cut(s) 2065, 2553
BstXI CCANNNNNNTGG 2 cut(s) 1533, 2671
BstYI RGATCY 2 cut(s) 2065, 2553
BsuI GTATCC 2 cut(s) 436, 1186
BsuRI GGCC 3 cut(s) 1010, 1286, 1665
BtgI CCRYGG 1 cut(s) 2557
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 1517
BtsCI GGATG 9 cut(s) 315, 412, 436, 542, 1708, 1738, 1759, 2387, 2440
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 4 cut(s) 818, 849, 1303, 2105
Cac8I GCNNGC 4 cut(s) 2503, 2529, 2602, 2606
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 5 cut(s) 145, 155, 222, 1008, 1390
CseI GACGC 3 cut(s) 56, 88, 2583
Csp6I GTAC 4 cut(s) 34, 193, 812, 2051
CspCI CAANNNNNGTGG 2 cut(s) 1498, 1533
CviQI GTAC 4 cut(s) 34, 193, 812, 2051
DdeI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2119, 2684
DpnI GATC 7 cut(s) 1005, 1038, 1674, 1857, 2067, 2537, 2555
DpnII GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 674
DrdI GACNNNNNNGTC 2 cut(s) 1496, 1810
DseDI GACNNNNNNGTC 2 cut(s) 1496, 1810
Eam1104I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2043, 2436
EarI CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2043, 2436
Ecl136II GAGCTC 2 cut(s) 182, 1548
Eco130I CCWWGG 1 cut(s) 2557
Eco147I AGGCCT 1 cut(s) 1286
Eco24I GRGCYC 3 cut(s) 184, 1550, 2157
Eco31I GGTCTC 1 cut(s) 252
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 4 cut(s) 145, 155, 222, 1390
Eco53kI GAGCTC 2 cut(s) 182, 1548
Eco57I CTGAAG 6 cut(s) 761, 2004, 2178, 2306, 2399, 2570
EcoICRI GAGCTC 2 cut(s) 182, 1548
EcoRI GAATTC 1 cut(s) 2409
EcoRII CCWGG 3 cut(s) 1032, 2391, 2663
EcoRV GATATC 1 cut(s) 1481
EcoT14I CCWWGG 1 cut(s) 2557
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 3 cut(s) 184, 1550, 2157
ErhI CCWWGG 1 cut(s) 2557
Esp3I CGTCTC 2 cut(s) 74, 573
FalI AAGNNNNNCTT 2 cut(s) 2022, 2054
FaqI GGGAC 6 cut(s) 35, 235, 1339, 1502, 1527, 2365
FauNDI CATATG 3 cut(s) 217, 752, 1207
FbaI TGATCA 1 cut(s) 2535
Fnu4HI GCNGC 6 cut(s) 491, 556, 710, 1372, 1408, 1599
FokI GGATG 9 cut(s) 302, 419, 423, 549, 1715, 1745, 1766, 2374, 2447
FriOI GRGCYC 3 cut(s) 184, 1550, 2157
Fsp4HI GCNGC 6 cut(s) 491, 556, 710, 1372, 1408, 1599
FspBI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2277, 2294, 2477, 2612
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 1 cut(s) 130
GluI GCNGC 6 cut(s) 491, 556, 710, 1372, 1408, 1599
HaeIII GGCC 3 cut(s) 1010, 1286, 1665
HapII CCGG 2 cut(s) 440, 1899
HgaI GACGC 3 cut(s) 56, 88, 2583
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 2575
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 2241
HindII GTYRAC 1 cut(s) 2241
HindIII AAGCTT 2 cut(s) 108, 2107
HinfI GANTC 8 cut(s) 6, 175, 472, 757, 1448, 1882, 2033, 2270
HpaII CCGG 2 cut(s) 440, 1899
HphI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2276, 2438
Hpy166II GTNNAC 5 cut(s) 36, 622, 657, 1390, 2241
Hpy188I TCNGA 8 cut(s) 23, 865, 1251, 1984, 2353, 2418, 2540, 2550
Hpy8I GTNNAC 5 cut(s) 36, 622, 657, 1390, 2241
Hpy99I CGWCG 2 cut(s) 104, 2577
HpyCH4III ACNGT 5 cut(s) 94, 197, 522, 924, 954
HpyCH4IV ACGT 7 cut(s) 263, 653, 1063, 1516, 1788, 2124, 2616
HpyF10VI GCNNNNNNNGC 3 cut(s) 683, 1298, 1598
HpyF3I CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2119, 2684
HpySE526I ACGT 7 cut(s) 263, 653, 1063, 1516, 1788, 2124, 2616
Hsp92I GRCGYC 1 cut(s) 2575
HspAI GCGC 1 cut(s) 129
Kpn2I TCCGGA 2 cut(s) 439, 1898
Ksp22I TGATCA 1 cut(s) 2535
Kzo9I GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
LguI GCTCTTC 1 cut(s) 2436
LmnI GCTCC 8 cut(s) 50, 267, 949, 1240, 1545, 1901, 2182, 2613
Lsp1109I GCAGC 4 cut(s) 502, 567, 721, 1383
LweI GCATC 6 cut(s) 445, 731, 1646, 1686, 1768, 1913
MaeI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2277, 2294, 2477, 2612
MaeII ACGT 7 cut(s) 263, 653, 1063, 1516, 1788, 2124, 2616
MalI GATC 7 cut(s) 1005, 1038, 1674, 1857, 2067, 2537, 2555
MbiI CCGCTC 1 cut(s) 1348
MboI GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
MfeI CAATTG 5 cut(s) 124, 161, 591, 2148, 2463
MflI RGATCY 2 cut(s) 2065, 2553
MhlI GDGCHC 6 cut(s) 184, 315, 946, 1550, 2157, 2293
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 6 cut(s) 370, 384, 1319, 1414, 1990, 2066
Mph1103I ATGCAT 1 cut(s) 438
MroI TCCGGA 2 cut(s) 439, 1898
MroXI GAANNNNTTC 1 cut(s) 2472
MseI TTAA 7 cut(s) 360, 399, 452, 876, 1950, 2111, 2226
MslI CAYNNNNRTG 4 cut(s) 1210, 1531, 1685, 1791
MspA1I CMGCKG 3 cut(s) 558, 1331, 1601
MspI CCGG 2 cut(s) 440, 1899
MspR9I CCNGG 3 cut(s) 1034, 2393, 2665
MunI CAATTG 5 cut(s) 124, 161, 591, 2148, 2463
MvaI CCWGG 3 cut(s) 1034, 2393, 2665
MwoI GCNNNNNNNGC 3 cut(s) 683, 1298, 1598
NcoI CCATGG 1 cut(s) 2557
NdeI CATATG 3 cut(s) 217, 752, 1207
NdeII GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
NlaIV GGNNCC 6 cut(s) 76, 223, 1392, 1918, 2178, 2460
NmeAIII GCCGAG 2 cut(s) 51, 1400
NmuCI GTSAC 9 cut(s) 88, 197, 649, 679, 1184, 1801, 1818, 2580, 2674
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 4 cut(s) 1185, 1446, 1690, 2505
NspV TTCGAA 1 cut(s) 2407
PaeI GCATGC 1 cut(s) 2505
PceI AGGCCT 1 cut(s) 1286
PciI ACATGT 2 cut(s) 1181, 1686
PciSI GCTCTTC 1 cut(s) 2436
PcsI WCGNNNNNNNCGW 1 cut(s) 2581
PdmI GAANNNNTTC 1 cut(s) 2472
PfeI GAWTC 7 cut(s) 6, 175, 757, 1448, 1882, 2033, 2270
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 6 cut(s) 492, 557, 711, 1373, 1409, 1600
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 2 cut(s) 264, 1789
PscI ACATGT 2 cut(s) 1181, 1686
PshAI GACNNNNGTC 1 cut(s) 2578
PsiI TTATAA 2 cut(s) 788, 2129
Psp124BI GAGCTC 2 cut(s) 184, 1550
Psp6I CCWGG 3 cut(s) 1032, 2391, 2663
PspGI CCWGG 3 cut(s) 1032, 2391, 2663
PspN4I GGNNCC 6 cut(s) 76, 223, 1392, 1918, 2178, 2460
PspPI GGNCC 5 cut(s) 145, 155, 222, 1008, 1390
PstI CTGCAG 3 cut(s) 492, 1362, 2335
PsuI RGATCY 2 cut(s) 2065, 2553
PvuII CAGCTG 2 cut(s) 558, 1331
RsaI GTAC 4 cut(s) 35, 194, 813, 2052
RsaNI GTAC 4 cut(s) 34, 193, 812, 2051
RseI CAYNNNNRTG 4 cut(s) 1210, 1531, 1685, 1791
SacI GAGCTC 2 cut(s) 184, 1550
SapI GCTCTTC 1 cut(s) 2436
SaqAI TTAA 7 cut(s) 360, 399, 452, 876, 1950, 2111, 2226
SatI GCNGC 6 cut(s) 491, 556, 710, 1372, 1408, 1599
Sau3AI GATC 7 cut(s) 1003, 1036, 1672, 1855, 2065, 2535, 2553
Sau96I GGNCC 5 cut(s) 145, 155, 222, 1008, 1390
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 1034, 2393, 2665
SduI GDGCHC 6 cut(s) 184, 315, 946, 1550, 2157, 2293
SfaNI GCATC 6 cut(s) 445, 731, 1646, 1686, 1768, 1913
SfcI CTRYAG 3 cut(s) 488, 1358, 2331
SfuI TTCGAA 1 cut(s) 2407
SinI GGWCC 4 cut(s) 145, 155, 222, 1390
SmiMI CAYNNNNRTG 4 cut(s) 1210, 1531, 1685, 1791
SphI GCATGC 1 cut(s) 2505
SseBI AGGCCT 1 cut(s) 1286
SsiI CCGC 6 cut(s) 158, 244, 1348, 1407, 1599, 1618
SspMI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2277, 2294, 2477, 2612
SstI GAGCTC 2 cut(s) 184, 1550
StuI AGGCCT 1 cut(s) 1286
StyD4I CCNGG 3 cut(s) 1032, 2391, 2663
StyI CCWWGG 1 cut(s) 2557
TaaI ACNGT 5 cut(s) 94, 197, 522, 924, 954
TaiI ACGT 7 cut(s) 266, 656, 1066, 1519, 1791, 2127, 2619
TaqI TCGA 9 cut(s) 12, 178, 548, 762, 1744, 1907, 1970, 2407, 2572
TaqII GACCGA 1 cut(s) 239
TatI WGTACW 2 cut(s) 811, 2050
TauI GCSGC 2 cut(s) 1410, 1601
TfiI GAWTC 7 cut(s) 6, 175, 757, 1448, 1882, 2033, 2270
Tru1I TTAA 7 cut(s) 360, 399, 452, 876, 1950, 2111, 2226
Tru9I TTAA 7 cut(s) 360, 399, 452, 876, 1950, 2111, 2226
TscAI CASTG 4 cut(s) 825, 849, 1303, 2105
TseFI GTSAC 9 cut(s) 88, 197, 649, 679, 1184, 1801, 1818, 2580, 2674
TseI GCWGC 4 cut(s) 490, 555, 709, 1371
Tsp45I GTSAC 9 cut(s) 88, 197, 649, 679, 1184, 1801, 1818, 2580, 2674
TspDTI ATGAA 8 cut(s) 423, 605, 654, 846, 852, 2354, 2356, 2451
TspGWI ACGGA 2 cut(s) 37, 49
TspRI CASTG 4 cut(s) 825, 849, 1303, 2105
VpaK11BI GGWCC 4 cut(s) 145, 155, 222, 1390
XapI RAATTY 8 cut(s) 117, 412, 1309, 1655, 2058, 2344, 2354, 2409
XbaI TCTAGA 3 cut(s) 1529, 2276, 2476
XceI RCATGY 4 cut(s) 1185, 1446, 1690, 2505
XmnI GAANNNNTTC 1 cut(s) 2472
XspI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2277, 2294, 2477, 2612
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.