RLG00000009436

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001399
Physical Location & Seq
Forward (+)
52819086 .. 52827245
8160 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009436

Sequence Viewer

Length: 2706 bp
ATGGGAATCTTCGAACCCTTCAGAGCAATCGGCTACACAACCACCTCCGTCCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCGTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTCGTCGGTCCGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGTATCGGGACTACACATTTGCTGCGTATGGGACTGATATAGCTGTTTTTAAGCGATCTCATCAGGTTGCTACGTGGAGCGCCCATAATGCGAAGGTCAATTTGTTGCTATTGTTTGGAGAGCATATCCTAAGTGTTGATGTTGAAGGTAATCTGTATATATGGACATTTAAAGGAATTGATGAGAATCTTGATCCGGTTGGACATATTAAGTTGGACGAAAATTTTACTCCTAGCTGTATAATGCATCCGGATACCTACTTAAATAAGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCAGCACAAAGAAAAAACTGTATGAGTTCAAGGGATGGAAATCTTCTATATGCAGCTGTGTTTCGTCTCCTGCGCTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATTTGATGAAGAGTTAGTTACGTTTGCCCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGCAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTGTTATAAGCATATGGAATCTCGAAAAAAGAAGGCTTCAATCAGTTATAAAAGAGGCACACGATGGTTCTATACTTTCACTGCACTTTTTTGTGAATGAACCAGTGTTAATGAGTTCATCATCAGACAATTCTATTAAAATGTGGATTTTTGACACAAGCGATGGAGATGCTCGTCTTTTACGGTTTAGAAGTGGTCATAGTGCTCCTCCACAATGCATAAGGTTTTATGCAAATGGGAGACATATTCTATCGGCTGGTCAGGATCGTGCCTTTCGCCTTTTCTCTATAATCCAGGATCAACAAAGTAGAGAGCTTTCTCAACGTCATATAACAAAACGAGCGAAGAAACTCAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTTGTTACATGTCATATGGATACCGCACAGGCATATGTTTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCCATCAGTGCATGTGGAAATTTTGCCATACTAGGGACCGCTGGTGGTTGGATTGAGCGGTTCAACCTGCAGTCAGGAAGCAGCCGAGGTAATTATGTGGATCCATCAGAAAGAAGCATCTGTGCCCATGACGGGGAAGTTGTTGGAATTGCTTGTGATTCAACTAATACTCACATGATAAGTGCTGGATATCACGGAAACATAAAGGTCTGGGATTTCAAGGGACGTGGAGTGCCATCTAGATGGGAAGTTGGGAGCTCTGTTGTCAGGATTGTATTCAATCGCTTGAATGGTCTTTTGGCTGTTGCGGCTGATGACTTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACTGATCGTGTTACAGACATGTGTTTTAGTGAGGATGGAAAATGGCTTTTGTCTTCCAGTATGGATGGAAGTCTTCGAGTTTGGGATGTTATCTTAGCAAGACAAATAGATGCGTTGCATGTTGATGTGTCTGTGACCGCATTGTCTCTATCACCGAATATGGATATTTTAGCGACCTCCCATGTTGATCAAAATGGGGTCTATCTTTGGGTGAATCAATCAATGTTTTCGGGAGCTTCAAAGGTAGATTCCTATGCAAGTGGAAAGCGAGTTGTGAGTGTTAAGTTGCCATCTGTCTCTTCGACAAAAGGTTCTGAAGCTGAGGATTCAGATGAGCCTGTTGGAAACTCTGAACAACTGAAAGATGCTTCTTCTCTTCCAAGTCCTGTACAGCAAATTCCAGATCTTGTTACTCTCTCATCATATCCTAAAAGCCAGTGGCAAAGCTTAATCAACTTAGACATTATAAAGGAACGAAATAAACCGATTGAGCCCCCAAAGAAACCCGAAAAGGCTCCTTTCTTTTTGCCTTCAGTTCCATCTCTTTCAGGAGAAATAATGTTTAAGCCTACTGGGTCGACAAATGAGGGGGCACAAGGTGGTGAAGAATCATCTAGAATAAAGGCTGCTCTAGCACCATCAATATTCCTTCAAGCACTTCAGTCATCTGCAGAAATGAAGAATTTTTCAGAATTTACTGATTTCATCAAAACTTTGTCTCCATCATCCCTGGATATGGAACTTCGAATTCTTCAGATTGTGGGTGATGAGGATGAAGAGCCAGAAAAGTTGCTTCCAATTGAACTGCTTCTTGACTATTTTATACACGAGACTGCATGCAGAAACAATTTTGACTTTGTACAAGCTGTGATCAGGGTTTTTCTGAAGATCCATGGCGAAACAATTCGACGCCAGTCACAACTACAGGATAAGGCAAGCAAGCTCCTAGACGTTCAATGCAACACATGGCAAAGAGTAGATAAAATGTTTCAAAGTGCTAGGTGCATGGTCACTTTTCTTAGCAATTCACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

902

Amino Acids

100.15

Weight (kDa)

6.77

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 298 3e-100 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 85 - 258 4.9e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 103 - 260 2.4e-15 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 123 - 353 7e-16 G protein beta WD-40 repeat protein
Beta-prop_RIG_2nd PF23775 134 - 287 2e-07 RIG second beta-propeller
WD40_Prp19 PF24814 144 - 295 4.5e-17 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 146 - 299 2.8e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 259 4.4e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 152 - 258 1.2e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 153 - 260 4.5e-12 WDR90/POC16, second beta-propeller
Beta-prop_WDR5 PF25175 154 - 295 1.4e-16 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 154 - 222 7.7e-06 TEP-1 second beta-propeller
Beta-prop_HPS5 PF23756 154 - 286 3.3e-07 HPS5 beta-propeller
WDR90_beta-prop_4th PF23342 155 - 273 1.6e-07 WDR90, 4th beta-propeller
Beta-prop_WDR3_1st PF25173 155 - 343 3.4e-25 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 157 - 298 8.1e-15 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 175 - 343 3.4e-07 Aladin seven-bladed propeller
WDR55 PF24796 183 - 295 5.5e-10 WDR55
Beta-prop_VPS8 PF23410 194 - 290 6.6e-07 Vps8 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 224 - 360 2.5e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 225 - 299 7.7e-09 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 226 - 299 5.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 227 - 343 3.6e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 239 - 343 4.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 239 - 355 2.7e-13 WDR5 beta-propeller domain
WD40_Prp19 PF24814 310 - 578 2.7e-12 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 636 3.8e-103 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 427 - 626 1.3e-20 WDR5 beta-propeller domain
WD40_Prp19 PF24814 468 - 616 1e-14 Prp19 WD40 domain
WDR55 PF24796 471 - 586 1.7e-07 WDR55
Beta-prop_WDR3_1st PF25173 476 - 621 5.1e-20 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 479 - 585 2.7e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 482 - 618 1.5e-08 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 516 - 615 4.8e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 516 - 631 7.5e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 517 - 597 5.1e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 518 - 592 2.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 528 - 627 6.3e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR75_1st PF23869 533 - 609 9.3e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 549 - 585 4.5e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 550 - 621 4.6e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 688 - 897 8.2e-54 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 746, 788, 2135
AasI GACNNNNNNGTC 1 cut(s) 1810
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1365
AccBSI CCGCTC 1 cut(s) 1348
AccI GTMKAC 1 cut(s) 2246
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 7 cut(s) 158, 1197, 1329, 1348, 1598, 1618, 1806
AclWI GGATC 6 cut(s) 377, 1011, 1044, 1385, 1398, 2551
AcsI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
AcuI CTGAAG 7 cut(s) 4, 99, 2004, 2184, 2312, 2405, 2573
AcyI GRCGYC 1 cut(s) 2578
AdeI CACNNNGTG 2 cut(s) 674, 2267
AfaI GTAC 2 cut(s) 2058, 2529
AfiI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
AflIII ACRYGT 2 cut(s) 1181, 1686
AjiI CACGTC 1 cut(s) 1517
AjnI CCWGG 2 cut(s) 1032, 2397
AjuI GAANNNNNNNTTGG 2 cut(s) 1571, 1603
Alw21I GWGCWC 3 cut(s) 184, 946, 1550
Alw26I GTCTC 7 cut(s) 573, 973, 1210, 1818, 1969, 2391, 2492
AlwI GGATC 6 cut(s) 377, 1011, 1044, 1385, 1398, 2551
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 2 cut(s) 1284, 1663
ApeKI GCWGC 6 cut(s) 212, 490, 555, 709, 1371, 2294
ApoI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 2 cut(s) 2475, 2571
AspLEI GCGC 4 cut(s) 69, 131, 272, 577
AspS9I GGNCC 3 cut(s) 145, 155, 1326
AsuHPI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2282, 2444
AsuII TTCGAA 2 cut(s) 12, 2413
AvaII GGWCC 3 cut(s) 145, 155, 1326
BaeGI GKGCMC 2 cut(s) 1417, 2263
BamHI GGATCC 1 cut(s) 1390
BanII GRGCYC 3 cut(s) 184, 1550, 2163
BauI CACGAG 2 cut(s) 669, 2495
BbsI GAAGAC 2 cut(s) 1713, 1733
Bbv12I GWGCWC 3 cut(s) 184, 946, 1550
BbvCI CCTCAGC 1 cut(s) 1989
BbvI GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BcgI CGANNNNNNTGC 4 cut(s) 890, 924, 1149, 1183
BciT130I CCWGG 2 cut(s) 1034, 2399
BciVI GTATCC 2 cut(s) 436, 1186
BclI TGATCA 2 cut(s) 1855, 2538
BcoDI GTCTC 7 cut(s) 573, 973, 1210, 1818, 1969, 2391, 2492
BfaI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2615, 2667
BfmI CTRYAG 4 cut(s) 488, 1358, 2337, 2591
BfoI RGCGCY 2 cut(s) 70, 273
BfuAI ACCTGC 1 cut(s) 1365
BfuI GTATCC 2 cut(s) 436, 1186
BglII AGATCT 1 cut(s) 2071
BisI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
BlsI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
Bme1390I CCNGG 2 cut(s) 1034, 2399
Bme18I GGWCC 3 cut(s) 145, 155, 1326
BmgBI CACGTC 1 cut(s) 1517
BmgT120I GGNCC 3 cut(s) 145, 155, 1326
BmiI GGNNCC 3 cut(s) 1327, 1392, 2184
BmrFI CCNGG 2 cut(s) 1034, 2399
BmrI ACTGGG 1 cut(s) 2250
BmsI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
BmuI ACTGGG 1 cut(s) 2250
BoxI GACNNNNGTC 1 cut(s) 2581
BpiI GAAGAC 2 cut(s) 1713, 1733
BpmI CTGGAG 1 cut(s) 747
Bpu10I CCTNAGC 1 cut(s) 1989
Bpu14I TTCGAA 2 cut(s) 12, 2413
BsaAI YACGTR 1 cut(s) 264
BsaBI GATNNNNATC 2 cut(s) 375, 541
BsaHI GRCGYC 1 cut(s) 2578
BsaJI CCNNGG 4 cut(s) 70, 1375, 2397, 2560
BsaWI WCCGGW 2 cut(s) 385, 439
BsaXI ACNNNNNCTCC 2 cut(s) 2371, 2401
Bsc4I CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
Bse1I ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2581
Bse3DI GCAATG 2 cut(s) 1128, 1177
Bse8I GATNNNNATC 2 cut(s) 375, 541
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 2 cut(s) 1034, 2399
BseDI CCNNGG 4 cut(s) 70, 1375, 2397, 2560
BseGI GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BseJI GATNNNNATC 2 cut(s) 375, 541
BseLI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
BseMI GCAATG 2 cut(s) 1128, 1177
BseMII CTCAG 2 cut(s) 1105, 1980
BseNI ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2581
BseRI GAGGAG 1 cut(s) 936
BseSI GKGCMC 2 cut(s) 1417, 2263
BseXI GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 2 cut(s) 1286, 1665
BsiHKAI GWGCWC 3 cut(s) 184, 946, 1550
BsiSI CCGG 2 cut(s) 386, 440
BslFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BslI CCNNNNNNNGG 4 cut(s) 1323, 1422, 1423, 2404
BsmAI GTCTC 7 cut(s) 573, 973, 1210, 1818, 1969, 2391, 2492
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BsnI GGCC 2 cut(s) 1286, 1665
Bsp119I TTCGAA 2 cut(s) 12, 2413
Bsp1286I GDGCHC 6 cut(s) 184, 946, 1417, 1550, 2163, 2263
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 2 cut(s) 2056, 2527
Bsp19I CCATGG 1 cut(s) 2560
BspACI CCGC 7 cut(s) 158, 1197, 1329, 1348, 1598, 1618, 1806
BspANI GGCC 2 cut(s) 1286, 1665
BspCNI CTCAG 2 cut(s) 1104, 1981
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 1327, 1392, 2184
BspMAI CTGCAG 3 cut(s) 492, 1362, 2341
BspMI ACCTGC 1 cut(s) 1365
BspPI GGATC 6 cut(s) 377, 1011, 1044, 1385, 1398, 2551
BspQI GCTCTTC 1 cut(s) 2439
BspT104I TTCGAA 2 cut(s) 12, 2413
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 1128, 1177
BsrGI TGTACA 2 cut(s) 2056, 2527
BsrI ACTGG 5 cut(s) 842, 1725, 2104, 2245, 2581
BssECI CCNNGG 4 cut(s) 70, 1375, 2397, 2560
BssNI GRCGYC 1 cut(s) 2578
BssSI CACGAG 2 cut(s) 669, 2495
BssT1I CCWWGG 1 cut(s) 2560
Bst2BI CACGAG 2 cut(s) 669, 2495
Bst2UI CCWGG 2 cut(s) 1034, 2399
Bst4CI ACNGT 4 cut(s) 94, 522, 924, 2700
Bst6I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2439
BstACI GRCGYC 1 cut(s) 2578
BstAUI TGTACA 2 cut(s) 2056, 2527
BstBAI YACGTR 1 cut(s) 264
BstBI TTCGAA 2 cut(s) 12, 2413
BstC8I GCNNGC 3 cut(s) 2506, 2605, 2609
BstDEI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2687
BstDSI CCRYGG 1 cut(s) 2560
BstF5I GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BstH2I RGCGCY 2 cut(s) 70, 273
BstHHI GCGC 4 cut(s) 69, 131, 272, 577
BstMAI GTCTC 7 cut(s) 573, 973, 1210, 1818, 1969, 2391, 2492
BstMWI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
BstNI CCWGG 2 cut(s) 1034, 2399
BstNSI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2508
BstPAI GACNNNNGTC 1 cut(s) 2581
BstSCI CCNGG 2 cut(s) 1032, 2397
BstSFI CTRYAG 4 cut(s) 488, 1358, 2337, 2591
BstSLI GKGCMC 2 cut(s) 1417, 2263
BstV1I GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
BstV2I GAAGAC 2 cut(s) 1713, 1733
BstX2I RGATCY 3 cut(s) 1390, 2071, 2556
BstXI CCANNNNNNTGG 1 cut(s) 1533
BstYI RGATCY 3 cut(s) 1390, 2071, 2556
BsuI GTATCC 2 cut(s) 436, 1186
BsuRI GGCC 2 cut(s) 1286, 1665
BtgI CCRYGG 1 cut(s) 2560
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 1517
BtsCI GGATG 7 cut(s) 436, 542, 1708, 1738, 1759, 2393, 2446
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 5 cut(s) 75, 818, 849, 1303, 2111
BveI ACCTGC 1 cut(s) 1365
Cac8I GCNNGC 3 cut(s) 2506, 2605, 2609
CfoI GCGC 4 cut(s) 69, 131, 272, 577
Cfr13I GGNCC 3 cut(s) 145, 155, 1326
CpoI CGGWCCG 1 cut(s) 155
CseI GACGC 2 cut(s) 88, 2586
Csp6I GTAC 2 cut(s) 2057, 2528
CspCI CAANNNNNGTGG 2 cut(s) 1498, 1533
CspI CGGWCCG 1 cut(s) 155
CviQI GTAC 2 cut(s) 2057, 2528
DdeI CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2687
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 2 cut(s) 674, 2267
DrdI GACNNNNNNGTC 1 cut(s) 1810
DseDI GACNNNNNNGTC 1 cut(s) 1810
Eam1104I CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2439
EarI CTCTTC 6 cut(s) 636, 720, 1101, 1972, 2049, 2439
Ecl136II GAGCTC 2 cut(s) 182, 1548
Eco130I CCWWGG 1 cut(s) 2560
Eco147I AGGCCT 1 cut(s) 1286
Eco24I GRGCYC 3 cut(s) 184, 1550, 2163
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 3 cut(s) 145, 155, 1326
Eco53kI GAGCTC 2 cut(s) 182, 1548
Eco57I CTGAAG 7 cut(s) 4, 99, 2004, 2184, 2312, 2405, 2573
EcoICRI GAGCTC 2 cut(s) 182, 1548
EcoRI GAATTC 1 cut(s) 2415
EcoRII CCWGG 2 cut(s) 1032, 2397
EcoRV GATATC 1 cut(s) 1481
EcoT14I CCWWGG 1 cut(s) 2560
EcoT22I ATGCAT 2 cut(s) 438, 959
EcoT38I GRGCYC 3 cut(s) 184, 1550, 2163
ErhI CCWWGG 1 cut(s) 2560
Esp3I CGTCTC 1 cut(s) 573
FaqI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
FauNDI CATATG 3 cut(s) 752, 1188, 1207
FbaI TGATCA 2 cut(s) 1855, 2538
FblI GTMKAC 1 cut(s) 2246
Fnu4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FokI GGATG 7 cut(s) 423, 549, 1715, 1745, 1766, 2380, 2453
FriOI GRGCYC 3 cut(s) 184, 1550, 2163
Fsp4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FspBI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2615, 2667
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 4 cut(s) 68, 130, 271, 576
GluI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 2 cut(s) 70, 273
HaeIII GGCC 2 cut(s) 1286, 1665
HapII CCGG 2 cut(s) 386, 440
HgaI GACGC 2 cut(s) 88, 2586
HhaI GCGC 4 cut(s) 69, 131, 272, 577
Hin1I GRCGYC 1 cut(s) 2578
Hin6I GCGC 4 cut(s) 67, 129, 270, 575
HinP1I GCGC 4 cut(s) 67, 129, 270, 575
HincII GTYRAC 1 cut(s) 2247
HindII GTYRAC 1 cut(s) 2247
HindIII AAGCTT 1 cut(s) 2113
HpaII CCGG 2 cut(s) 386, 440
HphI GGTGA 6 cut(s) 82, 1108, 1812, 1891, 2282, 2444
Hpy166II GTNNAC 2 cut(s) 622, 2247
Hpy8I GTNNAC 2 cut(s) 622, 2247
Hpy99I CGWCG 3 cut(s) 104, 155, 2580
HpyCH4III ACNGT 4 cut(s) 94, 522, 924, 2700
HpyCH4IV ACGT 5 cut(s) 263, 653, 1063, 1516, 2619
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
HpyF3I CTNAG 6 cut(s) 320, 1091, 1762, 1989, 2125, 2687
HpySE526I ACGT 5 cut(s) 263, 653, 1063, 1516, 2619
Hsp92I GRCGYC 1 cut(s) 2578
HspAI GCGC 4 cut(s) 67, 129, 270, 575
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 2 cut(s) 1855, 2538
LguI GCTCTTC 1 cut(s) 2439
LmnI GCTCC 7 cut(s) 267, 949, 1240, 1545, 1901, 2188, 2616
Lsp1109I GCAGC 6 cut(s) 199, 502, 567, 721, 1383, 2281
LweI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
MaeI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2615, 2667
MaeII ACGT 5 cut(s) 263, 653, 1063, 1516, 2619
MaeIII GTNAC 9 cut(s) 88, 649, 679, 1177, 1678, 1801, 2077, 2583, 2677
MbiI CCGCTC 1 cut(s) 1348
MfeI CAATTG 3 cut(s) 124, 161, 2466
MflI RGATCY 3 cut(s) 1390, 2071, 2556
MhlI GDGCHC 6 cut(s) 184, 946, 1417, 1550, 2163, 2263
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 5 cut(s) 370, 384, 1319, 1414, 1990
Mph1103I ATGCAT 2 cut(s) 438, 959
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 2 cut(s) 2475, 2571
MslI CAYNNNNRTG 2 cut(s) 1531, 1791
MspA1I CMGCKG 2 cut(s) 558, 1331
MspI CCGG 2 cut(s) 386, 440
MspR9I CCNGG 2 cut(s) 1034, 2399
MunI CAATTG 3 cut(s) 124, 161, 2466
MvaI CCWGG 2 cut(s) 1034, 2399
MwoI GCNNNNNNNGC 5 cut(s) 278, 683, 1298, 1598, 2300
NcoI CCATGG 1 cut(s) 2560
NdeI CATATG 3 cut(s) 752, 1188, 1207
NlaIV GGNNCC 3 cut(s) 1327, 1392, 2184
NmeAIII GCCGAG 2 cut(s) 51, 1400
NmuCI GTSAC 5 cut(s) 88, 679, 1801, 2583, 2677
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 2 cut(s) 438, 959
NspI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2508
NspV TTCGAA 2 cut(s) 12, 2413
PaeI GCATGC 1 cut(s) 2508
PceI AGGCCT 1 cut(s) 1286
PciI ACATGT 2 cut(s) 1181, 1686
PciSI GCTCTTC 1 cut(s) 2439
PdmI GAANNNNTTC 2 cut(s) 2475, 2571
PfeI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 1 cut(s) 264
PscI ACATGT 2 cut(s) 1181, 1686
PshAI GACNNNNGTC 1 cut(s) 2581
PsiI TTATAA 3 cut(s) 746, 788, 2135
Psp124BI GAGCTC 2 cut(s) 184, 1550
Psp6I CCWGG 2 cut(s) 1032, 2397
PspGI CCWGG 2 cut(s) 1032, 2397
PspN4I GGNNCC 3 cut(s) 1327, 1392, 2184
PspPI GGNCC 3 cut(s) 145, 155, 1326
PstI CTGCAG 3 cut(s) 492, 1362, 2341
PsuI RGATCY 3 cut(s) 1390, 2071, 2556
PvuII CAGCTG 1 cut(s) 558
RsaI GTAC 2 cut(s) 2058, 2529
RsaNI GTAC 2 cut(s) 2057, 2528
RseI CAYNNNNRTG 2 cut(s) 1531, 1791
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 184, 1550
SalI GTCGAC 1 cut(s) 2245
SapI GCTCTTC 1 cut(s) 2439
SatI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
Sau96I GGNCC 3 cut(s) 145, 155, 1326
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 2 cut(s) 1034, 2399
SduI GDGCHC 6 cut(s) 184, 946, 1417, 1550, 2163, 2263
SfaNI GCATC 7 cut(s) 445, 731, 898, 1416, 1646, 1768, 2023
SfcI CTRYAG 4 cut(s) 488, 1358, 2337, 2591
SfuI TTCGAA 2 cut(s) 12, 2413
SinI GGWCC 3 cut(s) 145, 155, 1326
SmiMI CAYNNNNRTG 2 cut(s) 1531, 1791
SphI GCATGC 1 cut(s) 2508
SseBI AGGCCT 1 cut(s) 1286
SsiI CCGC 7 cut(s) 158, 1197, 1329, 1348, 1598, 1618, 1806
SspI AATATT 1 cut(s) 2313
SspMI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2615, 2667
SstI GAGCTC 2 cut(s) 184, 1550
StuI AGGCCT 1 cut(s) 1286
StyD4I CCNGG 2 cut(s) 1032, 2397
StyI CCWWGG 1 cut(s) 2560
TaaI ACNGT 4 cut(s) 94, 522, 924, 2700
TaiI ACGT 5 cut(s) 266, 656, 1066, 1519, 2622
TaqI TCGA 8 cut(s) 12, 178, 762, 1744, 1970, 2246, 2413, 2575
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 2 cut(s) 2056, 2527
TauI GCSGC 1 cut(s) 1601
TfiI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
TscAI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
TseFI GTSAC 5 cut(s) 88, 679, 1801, 2583, 2677
TseI GCWGC 6 cut(s) 212, 490, 555, 709, 1371, 2294
Tsp45I GTSAC 5 cut(s) 88, 679, 1801, 2583, 2677
TspDTI ATGAA 7 cut(s) 605, 654, 846, 852, 2360, 2362, 2457
TspGWI ACGGA 3 cut(s) 37, 49, 1500
TspRI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
VpaK11BI GGWCC 3 cut(s) 145, 155, 1326
XapI RAATTY 9 cut(s) 117, 412, 612, 1309, 1655, 2064, 2350, 2360, 2415
XbaI TCTAGA 2 cut(s) 1529, 2282
XceI RCATGY 5 cut(s) 1185, 1305, 1690, 1790, 2508
XmiI GTMKAC 1 cut(s) 2246
XmnI GAANNNNTTC 2 cut(s) 2475, 2571
XspI CTAG 9 cut(s) 423, 578, 719, 1322, 1530, 2283, 2300, 2615, 2667
Zsp2I ATGCAT 2 cut(s) 438, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.