Rroxscaffold_5G00342290

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001399
Physical Location & Seq
Reverse (-)
11070691 .. 11072659
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342290.1

Sequence Viewer

Length: 852 bp
ATGGGTTCAGAGCTTGGGTCTCCGCAAGCCTCTTCGAACCCTTCAGAGTTCAGAGCAATCGGCTACACAACCACCTCCATCCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTCGTCGGTCGGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGTACCGGGACTACACATTCGCCGCATATGGGACTGATATAGCTGGTTTTAAGCGATCTCATCAGTCATTTGCATTTGTTCTGTTGCAAACCTTGGACGGACGTAACTTCTTTATATGGACATTTACAGGTCGCTACGTGGAGCATATCCTAAGTGTTGATGTTGAAGTACAAAGTGACCTCAAACTTCCATTGTTATCTGCACTGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCAGCACAAAGAAAAAACTTTATGAGTTCAAGGGATGGAAATCTTCTATTTGCAGCTGTGTTTCGTCTCCTGCACTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATATGATGAAGAGTTAATGACGTTTACTCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGCAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTGTTATAAGCATATGGAATCTCAAAAAAAGAAGGCTTCAATCTGTAACGCCCGGAAGGATTACGTTACAGCGCCGCAAGCCTCCGAACACGAGTCTGCAGCTTGTGCCACAAACCCCTGCCACAAAAGCTTGCAAGGCCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

283

Amino Acids

31.01

Weight (kDa)

9.73

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 40 - 96 1.2e-14 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 109 - 247 3.9e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 110 - 247 2.4e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 113 - 247 1.4e-13 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 131 - 243 4.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 135 - 253 2.5e-42 WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st PF23383 138 - 246 2.4e-06 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 139 - 248 4.6e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 140 - 245 2.8e-08 G protein beta WD-40 repeat protein
Beta-prop_RIG_2nd PF23775 140 - 250 1.8e-06 RIG second beta-propeller
Beta-prop_TEP1_2nd PF25047 141 - 209 8.9e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 142 - 247 7e-12 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 142 - 261 2.6e-08 WDR3 second beta-propeller domain
Beta-prop_HPS5 PF23756 142 - 248 1.8e-07 HPS5 beta-propeller
WDR55 PF24796 143 - 244 1.3e-07 WDR55
Beta-prop_EML PF23409 143 - 250 1.3e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 143 - 246 1.1e-14 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 144 - 246 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WDR90_beta-prop_4th PF23342 146 - 240 9.3e-08 WDR90, 4th beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 147 - 251 5e-13 WDR90/POC16, second beta-propeller
Beta-prop_WDR75_1st PF23869 165 - 251 3.4e-06 WD repeat-containing protein 75 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 713
Acc16I TGCGCA 1 cut(s) 160
AciI CCGC 3 cut(s) 23, 243, 781
AcsI RAATTY 2 cut(s) 147, 579
AcuI CTGAAG 2 cut(s) 27, 129
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 2 cut(s) 224, 390
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 4 cut(s) 386, 499, 633, 746
AluBI AGCT 8 cut(s) 13, 166, 212, 263, 460, 525, 808, 836
AluI AGCT 8 cut(s) 13, 166, 212, 263, 460, 525, 808, 836
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 2 cut(s) 24, 540
AoxI GGCC 1 cut(s) 843
ApeKI GCWGC 4 cut(s) 457, 522, 676, 805
ApoI RAATTY 2 cut(s) 147, 579
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AspLEI GCGC 3 cut(s) 99, 161, 780
AspS9I GGNCC 1 cut(s) 175
AsuC2I CCSGG 2 cut(s) 227, 759
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 214
BauI CACGAG 2 cut(s) 636, 796
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 4 cut(s) 469, 534, 688, 817
BccI CCATC 4 cut(s) 86, 498, 566, 665
BcnI CCSGG 2 cut(s) 227, 759
BcoDI GTCTC 2 cut(s) 24, 540
BfaI CTAG 2 cut(s) 545, 686
BfmI CTRYAG 2 cut(s) 455, 803
BfoI RGCGCY 2 cut(s) 100, 781
BisI GCNGC 6 cut(s) 243, 458, 523, 677, 781, 806
BlsI GCNGC 6 cut(s) 244, 459, 524, 678, 782, 807
Bme1390I CCNGG 2 cut(s) 227, 759
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmrFI CCNGG 2 cut(s) 227, 759
BmsI GCATC 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 35
BpuMI CCSGG 2 cut(s) 227, 759
BsaAI YACGTR 1 cut(s) 358
BsaBI GATNNNNATC 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 100, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse1I ACTGG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 508
BseDI CCNNGG 2 cut(s) 100, 312
BseGI GGATG 2 cut(s) 78, 509
BseJI GATNNNNATC 1 cut(s) 508
BseLI CCNNNNNNNGG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 429
BseXI GCAGC 4 cut(s) 469, 534, 688, 817
BsgI GTGCAG 2 cut(s) 405, 525
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 845
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 226, 759
BslFI GGGAC 2 cut(s) 242, 265
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 2 cut(s) 24, 540
BsmBI CGTCTC 1 cut(s) 540
BsmFI GGGAC 2 cut(s) 242, 265
BsnI GGCC 1 cut(s) 845
Bso31I GGTCTC 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 275
BspACI CCGC 3 cut(s) 23, 243, 781
BspANI GGCC 1 cut(s) 845
BspMAI CTGCAG 2 cut(s) 459, 807
BspT104I TTCGAA 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 24
BsrI ACTGG 1 cut(s) 429
BssECI CCNNGG 2 cut(s) 100, 312
BssMI GATC 1 cut(s) 275
BssSI CACGAG 2 cut(s) 636, 796
BssT1I CCWWGG 1 cut(s) 312
Bst2BI CACGAG 2 cut(s) 636, 796
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 3 cut(s) 37, 603, 687
BstAPI GCANNNNNTGC 1 cut(s) 811
BstBAI YACGTR 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 27, 785, 838
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 78, 509
BstH2I RGCGCY 2 cut(s) 100, 781
BstHHI GCGC 3 cut(s) 99, 161, 780
BstKTI GATC 1 cut(s) 278
BstMAI GTCTC 2 cut(s) 24, 540
BstMBI GATC 1 cut(s) 275
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 650, 784, 811, 833, 842
BstSCI CCNGG 2 cut(s) 225, 757
BstSFI CTRYAG 2 cut(s) 455, 803
BstV1I GCAGC 4 cut(s) 469, 534, 688, 817
BsuRI GGCC 1 cut(s) 845
BtsCI GGATG 2 cut(s) 78, 509
BtsIMutI CAGTG 2 cut(s) 105, 422
Cac8I GCNNGC 3 cut(s) 27, 785, 838
CfoI GCGC 3 cut(s) 99, 161, 780
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 223, 389
CviAII CATG 1 cut(s) 584
CviQI GTAC 2 cut(s) 223, 389
DdeI CTNAG 1 cut(s) 371
DpnI GATC 1 cut(s) 277
DpnII GATC 1 cut(s) 275
DraIII CACNNNGTG 1 cut(s) 641
Eam1104I CTCTTC 3 cut(s) 37, 603, 687
EarI CTCTTC 3 cut(s) 37, 603, 687
Ecl136II GAGCTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 312
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 1 cut(s) 214
Eco31I GGTCTC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 27, 129
EcoICRI GAGCTC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 1 cut(s) 587
FaqI GGGAC 2 cut(s) 242, 265
FatI CATG 1 cut(s) 583
FauNDI CATATG 2 cut(s) 247, 719
Fnu4HI GCNGC 6 cut(s) 243, 458, 523, 677, 781, 806
FokI GGATG 2 cut(s) 65, 516
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 6 cut(s) 243, 458, 523, 677, 781, 806
FspBI CTAG 2 cut(s) 545, 686
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 3 cut(s) 98, 160, 779
GluI GCNGC 6 cut(s) 243, 458, 523, 677, 781, 806
GsuI CTGGAG 1 cut(s) 714
HaeII RGCGCY 2 cut(s) 100, 781
HaeIII GGCC 1 cut(s) 845
HapII CCGG 2 cut(s) 226, 759
HgaI GACGC 1 cut(s) 118
HhaI GCGC 3 cut(s) 99, 161, 780
Hin1II CATG 1 cut(s) 587
Hin6I GCGC 3 cut(s) 97, 159, 778
HinP1I GCGC 3 cut(s) 97, 159, 778
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 205, 439, 724, 799
HpaII CCGG 2 cut(s) 226, 759
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 589, 624
Hpy188I TCNGA 4 cut(s) 10, 46, 53, 792
Hpy188III TCNNGA 2 cut(s) 443, 693
Hpy8I GTNNAC 2 cut(s) 589, 624
Hpy99I CGWCG 2 cut(s) 134, 185
HpyAV CCTTC 7 cut(s) 51, 94, 124, 151, 440, 732, 756
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 4 cut(s) 322, 357, 620, 770
HpyCH4V TGCA 9 cut(s) 293, 307, 422, 457, 522, 542, 557, 805, 840
HpyF10VI GCNNNNNNNGC 5 cut(s) 650, 784, 811, 833, 842
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 4 cut(s) 322, 357, 620, 770
Hsp92II CATG 1 cut(s) 587
HspAI GCGC 3 cut(s) 97, 159, 778
Kzo9I GATC 1 cut(s) 275
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 4 cut(s) 469, 534, 688, 817
LweI GCATC 1 cut(s) 698
MaeI CTAG 2 cut(s) 545, 686
MaeII ACGT 4 cut(s) 322, 357, 620, 770
MaeIII GTNAC 6 cut(s) 118, 323, 395, 646, 751, 771
MalI GATC 1 cut(s) 277
MboI GATC 1 cut(s) 275
MboII GAAGA 7 cut(s) 24, 211, 214, 504, 620, 674, 693
MfeI CAATTG 2 cut(s) 154, 191
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 4 cut(s) 147, 154, 191, 579
MlyI GAGTC 2 cut(s) 448, 808
MnlI CCTC 9 cut(s) 40, 85, 110, 188, 410, 632, 689, 690, 798
MseI TTAA 2 cut(s) 270, 614
MspA1I CMGCKG 1 cut(s) 525
MspI CCGG 2 cut(s) 226, 759
MspR9I CCNGG 2 cut(s) 227, 759
MunI CAATTG 2 cut(s) 154, 191
MwoI GCNNNNNNNGC 5 cut(s) 650, 784, 811, 833, 842
NciI CCSGG 2 cut(s) 227, 759
NdeI CATATG 2 cut(s) 247, 719
NdeII GATC 1 cut(s) 275
NlaIII CATG 1 cut(s) 587
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 118, 395, 646
NsbI TGCGCA 1 cut(s) 160
NspV TTCGAA 1 cut(s) 35
PceI AGGCCT 1 cut(s) 845
PfeI GAWTC 2 cut(s) 205, 724
PkrI GCNGC 6 cut(s) 244, 459, 524, 678, 782, 807
PleI GAGTC 2 cut(s) 447, 807
PpsI GAGTC 2 cut(s) 447, 807
Ppu21I YACGTR 1 cut(s) 358
PsiI TTATAA 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 175
PstI CTGCAG 2 cut(s) 459, 807
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 2 cut(s) 224, 390
RsaNI GTAC 2 cut(s) 223, 389
SacI GAGCTC 1 cut(s) 214
SaqAI TTAA 2 cut(s) 270, 614
SatI GCNGC 6 cut(s) 243, 458, 523, 677, 781, 806
Sau3AI GATC 1 cut(s) 275
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 2 cut(s) 448, 808
ScrFI CCNGG 2 cut(s) 227, 759
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 1 cut(s) 698
SfcI CTRYAG 2 cut(s) 455, 803
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 1 cut(s) 175
Sse9I AATT 4 cut(s) 147, 154, 191, 579
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 3 cut(s) 23, 243, 781
SspMI CTAG 2 cut(s) 545, 686
SstI GAGCTC 1 cut(s) 214
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 225, 757
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 4 cut(s) 325, 360, 623, 773
TaqI TCGA 2 cut(s) 35, 208
TaqII GACCGA 1 cut(s) 173
TasI AATT 4 cut(s) 147, 154, 191, 579
TatI WGTACW 1 cut(s) 388
TauI GCSGC 2 cut(s) 245, 783
TfiI GAWTC 2 cut(s) 205, 724
Tru1I TTAA 2 cut(s) 270, 614
Tru9I TTAA 2 cut(s) 270, 614
TscAI CASTG 2 cut(s) 112, 429
TseFI GTSAC 3 cut(s) 118, 395, 646
TseI GCWGC 4 cut(s) 457, 522, 676, 805
Tsp45I GTSAC 3 cut(s) 118, 395, 646
TspDTI ATGAA 2 cut(s) 572, 621
TspGWI ACGGA 2 cut(s) 79, 333
TspRI CASTG 2 cut(s) 112, 429
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 2 cut(s) 147, 579
XspI CTAG 2 cut(s) 545, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.