Rw0G011980

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00531
OG0001399
Physical Location & Seq
Reverse (-)
5183 .. 13419
8237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011980.1

Sequence Viewer

Length: 2121 bp
ATGGGAATCTTCGAGCCCTTCAGAGCAATCGGCTACACCACCACCTCCGACCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTGAGTCCGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGTACCTGGACTACACATTCGCTGCGTATGGGACTGATATAGCTGTTTTTAAGCGATCTCATCAGGTTGGTCGCTACGTGGAGCATATCCCAAGTGTTGATGTTGAAGGTAATCTGTATATATGGACATTTAAAGGAATTGATGAGAATCTTGATCTGGTTGGACATATTAAGTTGGACGAAAATTTTACTCCTAGCTGTATAACGCATCCGGATACCTACTTAAATAAGTCTCCTGCACTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATATGATGAAGAGTTAGTGACATTTACTCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCTGCACAGATGGGCAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTGTTATAAGCATATGGAATCTCAAAAAAAGAAGGCTTCAATCAGTTATAAAAGAGGCACACGATGGTTCTATACTTTCACTGCACTTTTTTATGAATGAACCAGTGTTAATGAGTTCATCATCAAACAATTCTATTAAAATGTGGATTTTTGACACAAGTGATGGAGATGCTTGTCTTTTATGGTTTAGAAGTGATCACAGTGCTCCTCCACAATGCATAAGGTTTTATGCAAATGGGAGACATATTCTATCGGCTGGTCAGGATCGTGCCTTTCGCCTTTTCTCTATAATCCAGGATCAACAAAGTAGAGAGCTTTCTCAACGTCATATAACAAAACGAGCAAAGAAACTCAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTTGTTACATGTCATATGGATACATATGTCTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCCATCAGTGAATGTGGAAATTTTGCCATACTAGGGACAGCTGGTGGTTGGATTGAGCGGATCAACCTGCAGTCAGGAAGCAGCCGATGTAGTTATGTGGACCCATCGGAAAGAAGCATCTGTGCCCATGATGGGGAAGTTGTTGGAATTGCTTGTGATTCAACTAATACTCACATGATAAGTGCTGGATATCATGGAGACATAAAGGTCTGGGATTTCAAGGGACGTGGAGTGCCATCTAGATGGGTAGTTGGGAGCTCTGTTGTCAGGATTGTATTCAATCGCTTGAATGGTCTTTTGGCTGTTGCGGCTGATGACTTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACTGATCTTTGGGATGTTATCTTAGCAAGACAAATCGATGCATTGCATGTTGATGTGTCTGTGACGGCACTGTCTCTATCACCAAATATGGATATTTTAGCGACCTCCCATGTTGATCAAAATGGGGGTCTAATAAAGACTGCTCTGGCACCATCAATATTCCTTCAAGCGCTTCAGTCTTCTGCAGAAATGAAGAATTTTTCAGAATTAATAAATTTCATCAAAACTTTGGCTCCATCGTCCCTGGATATGGAACTTCGAATTCTTCAGATTGTAGGTTATGAGGATGAAGAGCCAGAAAAGTTGCTTCCATTTGAACTGCTTCTCGACTATTTTATACACGAGACTGCATGCAGAAACAATTTTGACTTTGTGCAAAGCTGTGATCAGGGTTTTTCTGAAGGTATAGATATAATTCATTTCTGCATTCAACCATCTTTTTGCAATAAGTCATTAATTTCAATACATGGCGAAACAATTCGACGCCAGTCAAGATTACAGGATAAGGCAAGCAAGCTCCTAGACGTTCAATGCAACACATGGCAAAGAGTAGATAAAATGTTTCAGAGTGCCAGGTGCATGGTCACTTTTCTTAGCAATTCACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

706

Amino Acids

79.15

Weight (kDa)

6.37

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 139 1.1e-17 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 135 - 293 2.1e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 137 - 302 3.2e-13 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 139 - 248 4.1e-42 WDR36/Utp21 first beta-propeller
WDR55 PF24796 139 - 245 1.5e-08 WDR55
WD40_Gbeta PF25391 140 - 304 4e-08 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 140 - 248 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 141 - 298 2.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_VPS8 PF23410 144 - 241 4.5e-06 Vps8 beta-propeller
Beta-prop_WDR5 PF25175 144 - 303 4.5e-17 WDR5 beta-propeller domain
Beta-prop_EML PF23409 170 - 246 3.7e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 173 - 310 3.4e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 175 - 249 1.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 269 - 504 3.9e-62 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 346 - 513 1.4e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 371 - 508 1e-05 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 415 - 505 3.7e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 422 - 505 1e-05 WDR3 first beta-propeller domain
Utp21 PF04192 553 - 631 7.2e-07 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 596, 638
AasI GACNNNNNNGTC 2 cut(s) 1337, 1579
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1206
AccB1I GGYRCC 1 cut(s) 1657
AccBSI CCGCTC 1 cut(s) 1189
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 4 cut(s) 155, 1189, 1439, 1459
AclWI GGATC 3 cut(s) 861, 894, 1199
AcsI RAATTY 8 cut(s) 117, 370, 462, 1150, 1496, 1705, 1723, 1770
AcuI CTGAAG 5 cut(s) 4, 99, 1667, 1760, 1929
AcyI GRCGYC 1 cut(s) 1993
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 1 cut(s) 191
AfeI AGCGCT 1 cut(s) 1680
AfiI CCNNNNNNNGG 4 cut(s) 1164, 1263, 1264, 1759
AflIII ACRYGT 1 cut(s) 1031
AjiI CACGTC 1 cut(s) 1358
AjnI CCWGG 4 cut(s) 192, 882, 1752, 2081
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
AluBI AGCT 9 cut(s) 136, 179, 230, 384, 904, 1172, 1389, 1890, 2026
AluI AGCT 9 cut(s) 136, 179, 230, 384, 904, 1172, 1389, 1890, 2026
Alw21I GWGCWC 3 cut(s) 181, 796, 1391
Alw26I GTCTC 6 cut(s) 423, 823, 1051, 1323, 1587, 1847
AlwI GGATC 3 cut(s) 861, 894, 1199
Aor13HI TCCGGA 1 cut(s) 397
Aor51HI AGCGCT 1 cut(s) 1680
AoxI GGCC 2 cut(s) 1125, 1504
ApeKI GCWGC 3 cut(s) 209, 559, 1212
ApoI RAATTY 8 cut(s) 117, 370, 462, 1150, 1496, 1705, 1723, 1770
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 1621, 1653
AseI ATTAAT 2 cut(s) 1718, 1964
Asp700I GAANNNNTTC 2 cut(s) 1830, 1986
AspLEI GCGC 3 cut(s) 69, 131, 1681
AspS9I GGNCC 2 cut(s) 145, 1231
AsuHPI GGTGA 3 cut(s) 82, 958, 1581
AsuII TTCGAA 1 cut(s) 1768
AvaII GGWCC 2 cut(s) 145, 1231
BaeGI GKGCMC 1 cut(s) 1258
BaeI ACNNNNGTAYC 2 cut(s) 1035, 1068
BanI GGYRCC 1 cut(s) 1657
BanII GRGCYC 3 cut(s) 18, 181, 1391
BauI CACGAG 2 cut(s) 519, 1850
BbsI GAAGAC 1 cut(s) 1680
Bbv12I GWGCWC 3 cut(s) 181, 796, 1391
BbvI GCAGC 3 cut(s) 196, 571, 1224
BceAI ACGGC 1 cut(s) 1590
BcgI CGANNNNNNTGC 2 cut(s) 999, 1033
BciT130I CCWGG 4 cut(s) 194, 884, 1754, 2083
BciVI GTATCC 2 cut(s) 394, 1036
BclI TGATCA 3 cut(s) 784, 1624, 1894
BcoDI GTCTC 6 cut(s) 423, 823, 1051, 1323, 1587, 1847
BfaI CTAG 6 cut(s) 381, 428, 569, 1163, 1371, 2030
BfmI CTRYAG 2 cut(s) 1199, 1692
BfoI RGCGCY 2 cut(s) 70, 1682
BfuAI ACCTGC 1 cut(s) 1206
BfuI GTATCC 2 cut(s) 394, 1036
BisI GCNGC 4 cut(s) 210, 560, 1213, 1440
BlsI GCNGC 4 cut(s) 211, 561, 1214, 1441
Bme1390I CCNGG 4 cut(s) 194, 884, 1754, 2083
Bme18I GGWCC 2 cut(s) 145, 1231
BmgBI CACGTC 1 cut(s) 1358
BmgT120I GGNCC 2 cut(s) 145, 1231
BmiI GGNNCC 3 cut(s) 1233, 1659, 1743
BmrFI CCNGG 4 cut(s) 194, 884, 1754, 2083
BmsI GCATC 6 cut(s) 403, 581, 748, 1257, 1487, 1537
BoxI GACNNNNGTC 1 cut(s) 1996
BpiI GAAGAC 1 cut(s) 1680
BpmI CTGGAG 2 cut(s) 597, 1075
Bpu14I TTCGAA 1 cut(s) 1768
Bsa29I ATCGAT 1 cut(s) 1545
BsaAI YACGTR 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 1993
BsaJI CCNNGG 2 cut(s) 70, 1752
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 1726, 1756
Bsc4I CCNNNNNNNGG 4 cut(s) 1164, 1263, 1264, 1759
Bse1I ACTGG 2 cut(s) 692, 1996
Bse3DI GCAATG 3 cut(s) 978, 1027, 1550
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 4 cut(s) 194, 884, 1754, 2083
BseCI ATCGAT 1 cut(s) 1545
BseDI CCNNGG 2 cut(s) 70, 1752
BseGI GGATG 3 cut(s) 394, 1528, 1801
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 4 cut(s) 1164, 1263, 1264, 1759
BseMI GCAATG 3 cut(s) 978, 1027, 1550
BseMII CTCAG 2 cut(s) 140, 955
BseNI ACTGG 2 cut(s) 692, 1996
BseRI GAGGAG 1 cut(s) 786
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 3 cut(s) 196, 571, 1224
BsgI GTGCAG 3 cut(s) 408, 532, 656
BshFI GGCC 2 cut(s) 1127, 1506
BshNI GGYRCC 1 cut(s) 1657
BshVI ATCGAT 1 cut(s) 1545
BsiHKAI GWGCWC 3 cut(s) 181, 796, 1391
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 4 cut(s) 232, 1180, 1368, 1735
BslI CCNNNNNNNGG 4 cut(s) 1164, 1263, 1264, 1759
BsmAI GTCTC 6 cut(s) 423, 823, 1051, 1323, 1587, 1847
BsmFI GGGAC 4 cut(s) 232, 1180, 1368, 1735
BsmI GAATGC 1 cut(s) 1935
BsnI GGCC 2 cut(s) 1127, 1506
Bsp119I TTCGAA 1 cut(s) 1768
Bsp1286I GDGCHC 5 cut(s) 18, 181, 796, 1258, 1391
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
BspACI CCGC 4 cut(s) 155, 1189, 1439, 1459
BspANI GGCC 2 cut(s) 1127, 1506
BspCNI CTCAG 2 cut(s) 141, 954
BspDI ATCGAT 1 cut(s) 1545
BspEI TCCGGA 1 cut(s) 397
BspLI GGNNCC 3 cut(s) 1233, 1659, 1743
BspMAI CTGCAG 2 cut(s) 1203, 1696
BspMI ACCTGC 1 cut(s) 1206
BspPI GGATC 3 cut(s) 861, 894, 1199
BspQI GCTCTTC 1 cut(s) 1794
BspT104I TTCGAA 1 cut(s) 1768
BspT107I GGYRCC 1 cut(s) 1657
BsrBI CCGCTC 1 cut(s) 1189
BsrDI GCAATG 3 cut(s) 978, 1027, 1550
BsrI ACTGG 2 cut(s) 692, 1996
BssECI CCNNGG 2 cut(s) 70, 1752
BssMI GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
BssNI GRCGYC 1 cut(s) 1993
BssSI CACGAG 2 cut(s) 519, 1850
Bst2BI CACGAG 2 cut(s) 519, 1850
Bst2UI CCWGG 4 cut(s) 194, 884, 1754, 2083
Bst4CI ACNGT 4 cut(s) 94, 791, 1581, 2115
Bst6I CTCTTC 4 cut(s) 486, 570, 951, 1794
BstACI GRCGYC 1 cut(s) 1993
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 1768
BstC8I GCNNGC 3 cut(s) 1861, 2020, 2024
BstDEI CTNAG 4 cut(s) 149, 941, 1531, 2102
BstF5I GGATG 3 cut(s) 394, 1528, 1801
BstH2I RGCGCY 2 cut(s) 70, 1682
BstHHI GCGC 3 cut(s) 69, 131, 1681
BstKTI GATC 9 cut(s) 245, 343, 787, 856, 889, 1194, 1516, 1627, 1897
BstMAI GTCTC 6 cut(s) 423, 823, 1051, 1323, 1587, 1847
BstMBI GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
BstMWI GCNNNNNNNGC 2 cut(s) 533, 1439
BstNI CCWGG 4 cut(s) 194, 884, 1754, 2083
BstNSI RCATGY 3 cut(s) 1035, 1559, 1863
BstPAI GACNNNNGTC 1 cut(s) 1996
BstSCI CCNGG 4 cut(s) 192, 882, 1752, 2081
BstSFI CTRYAG 2 cut(s) 1199, 1692
BstSLI GKGCMC 1 cut(s) 1258
BstV1I GCAGC 3 cut(s) 196, 571, 1224
BstV2I GAAGAC 1 cut(s) 1680
BstXI CCANNNNNNTGG 2 cut(s) 1374, 2089
Bsu15I ATCGAT 1 cut(s) 1545
BsuI GTATCC 2 cut(s) 394, 1036
BsuRI GGCC 2 cut(s) 1127, 1506
BsuTUI ATCGAT 1 cut(s) 1545
BtrI CACGTC 1 cut(s) 1358
BtsCI GGATG 3 cut(s) 394, 1528, 1801
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 6 cut(s) 75, 668, 699, 796, 1144, 1577
BveI ACCTGC 1 cut(s) 1206
Cac8I GCNNGC 3 cut(s) 1861, 2020, 2024
CfoI GCGC 3 cut(s) 69, 131, 1681
Cfr13I GGNCC 2 cut(s) 145, 1231
ClaI ATCGAT 1 cut(s) 1545
CseI GACGC 2 cut(s) 88, 2001
Csp6I GTAC 1 cut(s) 190
CspCI CAANNNNNGTGG 2 cut(s) 1339, 1374
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 4 cut(s) 149, 941, 1531, 2102
DpnI GATC 9 cut(s) 244, 342, 786, 855, 888, 1193, 1515, 1626, 1896
DpnII GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
DraI TTTAAA 1 cut(s) 319
DraIII CACNNNGTG 1 cut(s) 524
DrdI GACNNNNNNGTC 2 cut(s) 1337, 1579
DseDI GACNNNNNNGTC 2 cut(s) 1337, 1579
Eam1104I CTCTTC 4 cut(s) 486, 570, 951, 1794
EarI CTCTTC 4 cut(s) 486, 570, 951, 1794
Ecl136II GAGCTC 2 cut(s) 179, 1389
Eco147I AGGCCT 1 cut(s) 1127
Eco24I GRGCYC 3 cut(s) 18, 181, 1391
Eco32I GATATC 1 cut(s) 1322
Eco47I GGWCC 2 cut(s) 145, 1231
Eco47III AGCGCT 1 cut(s) 1680
Eco53kI GAGCTC 2 cut(s) 179, 1389
Eco57I CTGAAG 5 cut(s) 4, 99, 1667, 1760, 1929
EcoICRI GAGCTC 2 cut(s) 179, 1389
EcoRI GAATTC 1 cut(s) 1770
EcoRII CCWGG 4 cut(s) 192, 882, 1752, 2081
EcoRV GATATC 1 cut(s) 1322
EcoT22I ATGCAT 2 cut(s) 809, 1552
EcoT38I GRGCYC 3 cut(s) 18, 181, 1391
FaqI GGGAC 4 cut(s) 232, 1180, 1368, 1735
FauNDI CATATG 3 cut(s) 602, 1038, 1048
FbaI TGATCA 3 cut(s) 784, 1624, 1894
Fnu4HI GCNGC 4 cut(s) 210, 560, 1213, 1440
FokI GGATG 3 cut(s) 381, 1535, 1808
FriOI GRGCYC 3 cut(s) 18, 181, 1391
Fsp4HI GCNGC 4 cut(s) 210, 560, 1213, 1440
FspBI CTAG 6 cut(s) 381, 428, 569, 1163, 1371, 2030
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 3 cut(s) 68, 130, 1680
GluI GCNGC 4 cut(s) 210, 560, 1213, 1440
GsuI CTGGAG 2 cut(s) 597, 1075
HaeII RGCGCY 2 cut(s) 70, 1682
HaeIII GGCC 2 cut(s) 1127, 1506
HapII CCGG 1 cut(s) 398
HgaI GACGC 2 cut(s) 88, 2001
HhaI GCGC 3 cut(s) 69, 131, 1681
Hin1I GRCGYC 1 cut(s) 1993
Hin6I GCGC 3 cut(s) 67, 129, 1679
HinP1I GCGC 3 cut(s) 67, 129, 1679
HinfI GANTC 6 cut(s) 6, 151, 172, 334, 607, 1289
HpaII CCGG 1 cut(s) 398
HphI GGTGA 3 cut(s) 82, 958, 1581
Hpy166II GTNNAC 2 cut(s) 472, 1231
Hpy188I TCNGA 8 cut(s) 23, 49, 1092, 1240, 1714, 1779, 1909, 2076
Hpy8I GTNNAC 2 cut(s) 472, 1231
Hpy99I CGWCG 2 cut(s) 104, 1995
HpyCH4III ACNGT 4 cut(s) 94, 791, 1581, 2115
HpyCH4IV ACGT 4 cut(s) 264, 913, 1357, 2034
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 1439
HpyF3I CTNAG 4 cut(s) 149, 941, 1531, 2102
HpySE526I ACGT 4 cut(s) 264, 913, 1357, 2034
Hsp92I GRCGYC 1 cut(s) 1993
HspAI GCGC 3 cut(s) 67, 129, 1679
Kpn2I TCCGGA 1 cut(s) 397
Ksp22I TGATCA 3 cut(s) 784, 1624, 1894
Kzo9I GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
LguI GCTCTTC 1 cut(s) 1794
LmnI GCTCC 6 cut(s) 268, 799, 1081, 1386, 1747, 2031
Lsp1109I GCAGC 3 cut(s) 196, 571, 1224
LweI GCATC 6 cut(s) 403, 581, 748, 1257, 1487, 1537
MaeI CTAG 6 cut(s) 381, 428, 569, 1163, 1371, 2030
MaeII ACGT 4 cut(s) 264, 913, 1357, 2034
MaeIII GTNAC 6 cut(s) 88, 499, 529, 1027, 1570, 2092
MalI GATC 9 cut(s) 244, 342, 786, 855, 888, 1193, 1515, 1626, 1896
MbiI CCGCTC 1 cut(s) 1189
MboI GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
MfeI CAATTG 2 cut(s) 124, 158
MhlI GDGCHC 5 cut(s) 18, 181, 796, 1258, 1391
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 5 cut(s) 72, 328, 342, 1160, 1255
Mph1103I ATGCAT 2 cut(s) 809, 1552
MroI TCCGGA 1 cut(s) 397
MroXI GAANNNNTTC 2 cut(s) 1830, 1986
MseI TTAA 9 cut(s) 237, 318, 357, 410, 698, 726, 1451, 1718, 1964
MslI CAYNNNNRTG 2 cut(s) 1372, 1560
MspA1I CMGCKG 1 cut(s) 1172
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 4 cut(s) 194, 884, 1754, 2083
MunI CAATTG 2 cut(s) 124, 158
Mva1269I GAATGC 1 cut(s) 1935
MvaI CCWGG 4 cut(s) 194, 884, 1754, 2083
MwoI GCNNNNNNNGC 2 cut(s) 533, 1439
NdeI CATATG 3 cut(s) 602, 1038, 1048
NdeII GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
NlaIV GGNNCC 3 cut(s) 1233, 1659, 1743
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 5 cut(s) 88, 499, 529, 1570, 2092
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 2 cut(s) 809, 1552
NspI RCATGY 3 cut(s) 1035, 1559, 1863
NspV TTCGAA 1 cut(s) 1768
PaeI GCATGC 1 cut(s) 1863
PceI AGGCCT 1 cut(s) 1127
PciI ACATGT 1 cut(s) 1031
PciSI GCTCTTC 1 cut(s) 1794
PctI GAATGC 1 cut(s) 1935
PdmI GAANNNNTTC 2 cut(s) 1830, 1986
PfeI GAWTC 5 cut(s) 6, 172, 334, 607, 1289
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 4 cut(s) 211, 561, 1214, 1441
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Ppu21I YACGTR 1 cut(s) 265
PscI ACATGT 1 cut(s) 1031
PshAI GACNNNNGTC 1 cut(s) 1996
PshBI ATTAAT 2 cut(s) 1718, 1964
PsiI TTATAA 2 cut(s) 596, 638
Psp124BI GAGCTC 2 cut(s) 181, 1391
Psp6I CCWGG 4 cut(s) 192, 882, 1752, 2081
PspGI CCWGG 4 cut(s) 192, 882, 1752, 2081
PspN4I GGNNCC 3 cut(s) 1233, 1659, 1743
PspPI GGNCC 2 cut(s) 145, 1231
PstI CTGCAG 2 cut(s) 1203, 1696
PvuII CAGCTG 1 cut(s) 1172
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 1372, 1560
SacI GAGCTC 2 cut(s) 181, 1391
SapI GCTCTTC 1 cut(s) 1794
SaqAI TTAA 9 cut(s) 237, 318, 357, 410, 698, 726, 1451, 1718, 1964
SatI GCNGC 4 cut(s) 210, 560, 1213, 1440
Sau3AI GATC 9 cut(s) 242, 340, 784, 853, 886, 1191, 1513, 1624, 1894
Sau96I GGNCC 2 cut(s) 145, 1231
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 4 cut(s) 194, 884, 1754, 2083
SduI GDGCHC 5 cut(s) 18, 181, 796, 1258, 1391
SfaNI GCATC 6 cut(s) 403, 581, 748, 1257, 1487, 1537
SfcI CTRYAG 2 cut(s) 1199, 1692
SfuI TTCGAA 1 cut(s) 1768
SinI GGWCC 2 cut(s) 145, 1231
SmiMI CAYNNNNRTG 2 cut(s) 1372, 1560
SphI GCATGC 1 cut(s) 1863
SseBI AGGCCT 1 cut(s) 1127
SsiI CCGC 4 cut(s) 155, 1189, 1439, 1459
SspI AATATT 1 cut(s) 1668
SspMI CTAG 6 cut(s) 381, 428, 569, 1163, 1371, 2030
SstI GAGCTC 2 cut(s) 181, 1391
StuI AGGCCT 1 cut(s) 1127
StyD4I CCNGG 4 cut(s) 192, 882, 1752, 2081
TaaI ACNGT 4 cut(s) 94, 791, 1581, 2115
TaiI ACGT 4 cut(s) 267, 916, 1360, 2037
TaqI TCGA 6 cut(s) 12, 175, 1545, 1768, 1836, 1990
TauI GCSGC 1 cut(s) 1442
TfiI GAWTC 5 cut(s) 6, 172, 334, 607, 1289
Tru1I TTAA 9 cut(s) 237, 318, 357, 410, 698, 726, 1451, 1718, 1964
Tru9I TTAA 9 cut(s) 237, 318, 357, 410, 698, 726, 1451, 1718, 1964
TscAI CASTG 6 cut(s) 82, 675, 699, 796, 1144, 1584
TseFI GTSAC 5 cut(s) 88, 499, 529, 1570, 2092
TseI GCWGC 3 cut(s) 209, 559, 1212
Tsp45I GTSAC 5 cut(s) 88, 499, 529, 1570, 2092
TspDTI ATGAA 9 cut(s) 455, 504, 696, 698, 702, 1715, 1717, 1812, 1916
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 6 cut(s) 82, 675, 699, 796, 1144, 1584
VpaK11BI GGWCC 2 cut(s) 145, 1231
VspI ATTAAT 2 cut(s) 1718, 1964
XapI RAATTY 8 cut(s) 117, 370, 462, 1150, 1496, 1705, 1723, 1770
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 3 cut(s) 1035, 1559, 1863
XmnI GAANNNNTTC 2 cut(s) 1830, 1986
XspI CTAG 6 cut(s) 381, 428, 569, 1163, 1371, 2030
Zsp2I ATGCAT 2 cut(s) 809, 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.