MD13G1232300.v1.1

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001399
Physical Location & Seq
Reverse (-)
23086572 .. 23095910
9339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1232300.v1.1.491

Sequence Viewer

Length: 2712 bp
ATGGGAATATTCGAGCCGTTCAGAGCAATCGGCTACACAACCAGCTCCGTGCCCTTCTCTGTTCAGCGCCTCGGCACCGAAACCTTCGTCACTGTCTCCGTCGGCAAGGCCTTCCAAATTTACAACTGTGCAAAGCTCAGTCTCGTCCTCGTTGGTCCACAATTGCCAAAGAAGATTCGAGCTCTTGCTTCCTACCGTGATTATACATTTGCTGCATATGGGAATGAGATAGCCATTTTTAAGCGAGCTCATCAGGCAGCAACATGGAGCAGCCATAATGCCAAGGTCATTTCTTTGCTATTGTTTGGAGAGCATATCCTAAGTGTTGATGTTGAAGGTAATCTGTTTATATGGGCATTCAAAGGAATTGAGGAGAACCTTACTCCGATCGGACATGTTATGTTAGCCAACAACTTTAGTCCTAGCTGTATAATGCATCCGGATACCTACCTAAATAAGATTATTATTGGGAGTCAGGAAGGTTCTCTTCAACTTTGGAACATCAGTACAAAGAAAAAACTCTATGAGTTCAAGGGATGGAATTCTTCAATCTGCAGTTGTGTTTCATCTCCTGCACTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAATATTCATGTTCACAACATTCGTTACGATGAAGAGCTAGTCACATTTTCTCATTCGGCACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGAAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTATCATAAGCATATGGAATCTTGAAAAAAGAAGGCTTCAATCAGTAATAAGAGATGCCCATGATGGTTCCATACTATCACTTCACTTTCTTGTGAACGAACCCGTGCTAATGAGTTCATCATCTGACAATTCTATCAAAATGTGGATTTTTGACACAACTGATGGAGATCCTCGACTTTTACGATTCAGAAGTGGTCATAGTGCTCCTCCACGATGCATAAGGTTTTATGCGAATGGGAGACACATTCTATCGGCTGGTCAGGACCGTGCATTTCGCCTTTTCTCTGTAATGCAGGATCAGCAAAGTAGAGAGCTTTCTCAGCATCACATATCCAAACGAGCAAAGAAACTGAAGATGAAGGAAGAAGAGATAAAACTGAAGCCTGTCATCTCTTTTGACTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTGGTTACATGCCATATGGATACTGCAGAGGCATATGTATGGAGACTTCAAAACTTTGTTCTTGGTGAGCATATTCTGAAGCCAAGACCAGAAAACCCCACACCAGTGAAGGCTTGTGCCATCAGTGCATGTGGCAATTTTGCTGTACTAGGGACAGTGGATGGTTGGGTTGAGCGGTTCAACCTCCAATCAGGAATCAGTCGAGGCAGTTACATGGACACGTCAGAAAGAAGAAGCTTTGCCCATGATGGGGAAGTGGTTGGAATTGCTTGTGATTCAACAAATACTCTAATGATAAGTGCAGGATATCATGGAGACATAAAGGTTTGGAATTTCAAGGGGCGTGATGTAAAATCTAGATGGGAAGTTGGGTGCTCTGTTGTCAAGATTGTATACAATCGTTTGAATGGTCTTTTGGCTGTTGTGGCCGATGACTTAATCATCCGTTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACAGATCGTGTTACAGACATGTGTTTTAGTGAGGATGGGAAATGGCTTTTGTCATCAAGTATGGATGGAAGTCTTCGAGTCTGGGATGTTATTTTAGCAAGACAAATAGATGCGTTGCATGTTGACGTGTCTATAACAGCATTGTCTCTGTCACCGAATATGGATGTTCTAGCGACTGCTCATATCGACCAAAATGGGGTCTATCTTTGGGTAAATCAATCGATGTTTTGTGGAGCTTCAAAAGTTGATTCTTATGCAAAGGAAGTTAGGAGTGTTAAGTTGCCATCTATCTCTTCAACAAAAGGTTCTCAAGATGAGGACTCTGACGAGCCTATTGTGGACCATCCGCAGTCTAAGGATGCTCCTGCTTTTACAACTCTTGACCTGCAAATTCCAGATCTTGTTACTCTCTCATTATTGCCTAAAAGCCAGTGGCAAAGCTTAATCAATTTAGACACTATAAAGGAACGAAATAAACCAATTGAACCGCCAAAAAAACCTGAAAGGGCTCCTTTCTTTTTGCCTTCAATTCCCTCCTTTTCAGGAGAAATATTATTTAAGTCCACTGGATCAGAAAATGAGGAGGCCAAAGGTGATAACGTAGAAGACAATAGAATAAAATCTGGTCTGGCACCATCACAATTCTTGCAACATCTTCAGTCGTCCGCAGAAATGAAGAATTTTTCAGCATTTACCGACTATATCAAAAGTTTGTCTCCATCGACTCTGGATATGGAACTCCAAATGCTTCGGATTGTTGATGATGAGGAGGAAGAACATGAAAGCCGGCCAGAGTTGCTTTCAATCGAACTGCTTCTTGATTATTTTATACATGAGACTTCATGTAGAAACAATTTTGACTTTGTACAAGCTGTAATCAAGGTGTTTCTCAAGATCCATGGCGAAACAATTCGGTGCCAATCAAGACTGCAGGACAAGGCCCGGGAGTTCCTAGATATTCAATGCAAAACATGGCAAAGAGTAGAAAAACTGTTTCAAAGTACAAGTTGTGTGGTCGCTTTTCTTAGCAATTCACGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

904

Amino Acids

101.24

Weight (kDa)

6.38

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 298 5.6e-100 WDR36/Utp21 first beta-propeller
Beta-prop_RIG_2nd PF23775 95 - 287 9.7e-08 RIG second beta-propeller
Beta-prop_WDR5 PF25175 106 - 298 1.1e-17 WDR5 beta-propeller domain
WD40_Gbeta PF25391 143 - 242 1.1e-07 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 145 - 254 1e-05 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 145 - 354 5.4e-15 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 145 - 295 3.3e-16 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 146 - 299 6.3e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 259 5.3e-06 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 151 - 258 2.2e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 152 - 258 2.6e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 152 - 260 2.4e-12 WDR90/POC16, second beta-propeller
WDR55 PF24796 152 - 255 1.1e-07 WDR55
Beta-prop_HPS5 PF23756 153 - 275 1.3e-07 HPS5 beta-propeller
WDR90_beta-prop_4th PF23342 155 - 274 2.1e-08 WDR90, 4th beta-propeller
Beta-prop_WDR3_1st PF25173 156 - 299 7e-23 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 158 - 296 2.6e-14 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 175 - 344 2.4e-06 Aladin seven-bladed propeller
WDR55 PF24796 183 - 295 9.1e-10 WDR55
Beta-prop_VPS8 PF23410 194 - 290 4e-07 Vps8 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 224 - 369 2.8e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 225 - 299 9.5e-09 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 226 - 300 2.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 227 - 359 4.2e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 227 - 345 4.3e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 239 - 347 7e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 635 4.2e-103 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 426 - 626 5.9e-19 WDR5 beta-propeller domain
WD40_Prp19 PF24814 427 - 625 6.8e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 428 - 648 1.6e-16 THOC3 beta-propeller domain
WDR55 PF24796 472 - 586 8.2e-07 WDR55
Beta-prop_WDR3_1st PF25173 473 - 621 1.4e-19 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 478 - 585 6.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 479 - 619 4.2e-07 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 507 - 616 5.5e-07 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 518 - 596 8.1e-11 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 518 - 632 1.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR75_1st PF23869 537 - 609 9.8e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 549 - 585 4.5e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 550 - 621 8.4e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 688 - 899 2.6e-54 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2061
AccB1I GGYRCC 3 cut(s) 74, 2299, 2583
AccBSI CCGCTC 1 cut(s) 1348
AccI GTMKAC 1 cut(s) 1566
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 4 cut(s) 1348, 2015, 2156, 2334
AclWI GGATC 4 cut(s) 902, 1044, 2245, 2557
AcoI YGGCCR 2 cut(s) 1599, 2456
AcsI RAATTY 6 cut(s) 117, 541, 1504, 1655, 2058, 2347
AcuI CTGAAG 4 cut(s) 1112, 1139, 1271, 2309
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 4 cut(s) 508, 1320, 2535, 2671
AfiI CCNNNNNNNGG 3 cut(s) 1422, 1423, 1864
AflIII ACRYGT 4 cut(s) 394, 1392, 1686, 1794
AjiI CACGTC 2 cut(s) 1395, 1795
AjuI GAANNNNNNNTTGG 2 cut(s) 1571, 1603
AleI CACNNNNGTG 1 cut(s) 1277
Alw21I GWGCWC 4 cut(s) 184, 250, 946, 1550
Alw26I GTCTC 8 cut(s) 100, 146, 973, 1210, 1482, 1818, 2388, 2498
AlwI GGATC 4 cut(s) 902, 1044, 2245, 2557
Ama87I CYCGRG 1 cut(s) 2610
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 6 cut(s) 108, 1599, 1663, 2253, 2456, 2607
ApeKI GCWGC 3 cut(s) 212, 257, 270
ApoI RAATTY 6 cut(s) 117, 541, 1504, 1655, 2058, 2347
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
Asp700I GAANNNNTTC 2 cut(s) 2481, 2577
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 4 cut(s) 155, 1003, 2008, 2608
AsuC2I CCSGG 2 cut(s) 2611, 2612
AsuHPI GGTGA 3 cut(s) 1250, 1812, 2273
AvaI CYCGRG 1 cut(s) 2610
AvaII GGWCC 3 cut(s) 155, 1003, 2008
BaeGI GKGCMC 1 cut(s) 54
BanI GGYRCC 3 cut(s) 74, 2299, 2583
BanII GRGCYC 3 cut(s) 184, 250, 2179
BarI GAAGNNNNNNTAC 2 cut(s) 1203, 1235
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 2 cut(s) 1733, 2280
Bbv12I GWGCWC 4 cut(s) 184, 250, 946, 1550
BbvI GCAGC 3 cut(s) 199, 269, 282
BcgI CGANNNNNNTGC 2 cut(s) 1149, 1183
BciVI GTATCC 2 cut(s) 436, 1186
BcnI CCSGG 2 cut(s) 2611, 2612
BcoDI GTCTC 8 cut(s) 100, 146, 973, 1210, 1482, 1818, 2388, 2498
BfaI CTAG 8 cut(s) 423, 578, 647, 719, 1322, 1530, 1838, 2621
BfmI CTRYAG 3 cut(s) 553, 1197, 2597
BfoI RGCGCY 1 cut(s) 70
BfuAI ACCTGC 1 cut(s) 2061
BfuI GTATCC 2 cut(s) 436, 1186
BglII AGATCT 1 cut(s) 2065
BisI GCNGC 3 cut(s) 213, 258, 271
BlsI GCNGC 3 cut(s) 214, 259, 272
Bme1390I CCNGG 2 cut(s) 2611, 2612
Bme18I GGWCC 3 cut(s) 155, 1003, 2008
BmeT110I CYCGRG 1 cut(s) 2610
BmgBI CACGTC 2 cut(s) 1395, 1795
BmgT120I GGNCC 4 cut(s) 155, 1003, 2008, 2608
BmiI GGNNCC 5 cut(s) 76, 808, 2178, 2301, 2585
BmrFI CCNGG 2 cut(s) 2611, 2612
BmsI GCATC 8 cut(s) 445, 731, 784, 944, 1072, 1646, 1768, 2017
BpiI GAAGAC 2 cut(s) 1733, 2280
BpmI CTGGAG 1 cut(s) 747
BpuEI CTTGAG 2 cut(s) 1962, 2543
BpuMI CCSGG 2 cut(s) 2611, 2612
Bsa29I ATCGAT 1 cut(s) 1889
BsaBI GATNNNNATC 1 cut(s) 906
BsaJI CCNNGG 4 cut(s) 70, 282, 2566, 2610
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 3 cut(s) 1422, 1423, 1864
Bse118I RCCGGY 1 cut(s) 2454
Bse1I ACTGG 3 cut(s) 1277, 2098, 2239
Bse3DI GCAATG 1 cut(s) 1177
Bse8I GATNNNNATC 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 439
BseCI ATCGAT 1 cut(s) 1889
BseDI CCNNGG 4 cut(s) 70, 282, 2566, 2610
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 3 cut(s) 1422, 1423, 1864
BseMI GCAATG 1 cut(s) 1177
BseMII CTCAG 2 cut(s) 151, 1073
BseNI ACTGG 3 cut(s) 1277, 2098, 2239
BseRI GAGGAG 4 cut(s) 386, 936, 2264, 2450
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 3 cut(s) 199, 269, 282
BsgI GTGCAG 2 cut(s) 558, 1494
Bsh1285I CGRYCG 1 cut(s) 390
BshFI GGCC 6 cut(s) 110, 1601, 1665, 2255, 2458, 2609
BshNI GGYRCC 3 cut(s) 74, 2299, 2583
BshVI ATCGAT 1 cut(s) 1889
BsiEI CGRYCG 1 cut(s) 390
BsiHKAI GWGCWC 4 cut(s) 184, 250, 946, 1550
BsiHKCI CYCGRG 1 cut(s) 2610
BsiSI CCGG 3 cut(s) 440, 2455, 2611
BslFI GGGAC 1 cut(s) 1339
BslI CCNNNNNNNGG 3 cut(s) 1422, 1423, 1864
BsmAI GTCTC 8 cut(s) 100, 146, 973, 1210, 1482, 1818, 2388, 2498
BsmFI GGGAC 1 cut(s) 1339
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 6 cut(s) 110, 1601, 1665, 2255, 2458, 2609
BsoBI CYCGRG 1 cut(s) 2610
Bsp1286I GDGCHC 6 cut(s) 54, 184, 250, 946, 1550, 2179
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 2533
Bsp143I GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
Bsp19I CCATGG 1 cut(s) 2566
BspACI CCGC 4 cut(s) 1348, 2015, 2156, 2334
BspANI GGCC 6 cut(s) 110, 1601, 1665, 2255, 2458, 2609
BspCNI CTCAG 2 cut(s) 150, 1072
BspDI ATCGAT 1 cut(s) 1889
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 5 cut(s) 76, 808, 2178, 2301, 2585
BspMAI CTGCAG 3 cut(s) 557, 1201, 2601
BspMI ACCTGC 1 cut(s) 2061
BspPI GGATC 4 cut(s) 902, 1044, 2245, 2557
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 3 cut(s) 74, 2299, 2583
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 1 cut(s) 1177
BsrFI RCCGGY 1 cut(s) 2454
BsrGI TGTACA 1 cut(s) 2533
BsrI ACTGG 3 cut(s) 1277, 2098, 2239
BssAI RCCGGY 1 cut(s) 2454
BssECI CCNNGG 4 cut(s) 70, 282, 2566, 2610
BssMI GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
BssNAI GTATAC 1 cut(s) 1567
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 2 cut(s) 282, 2566
Bst1107I GTATAC 1 cut(s) 1567
Bst2BI CACGAG 1 cut(s) 669
Bst4CI ACNGT 8 cut(s) 94, 128, 197, 1007, 1142, 1330, 2661, 2706
Bst6I CTCTTC 5 cut(s) 492, 636, 720, 1101, 1966
BstAPI GCANNNNNTGC 1 cut(s) 674
BstAUI TGTACA 1 cut(s) 2533
BstC8I GCNNGC 2 cut(s) 246, 2456
BstDEI CTNAG 5 cut(s) 137, 320, 1059, 2022, 2693
BstDSI CCRYGG 1 cut(s) 2566
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 7 cut(s) 390, 910, 1039, 1675, 2068, 2240, 2565
BstMAI GTCTC 8 cut(s) 100, 146, 973, 1210, 1482, 1818, 2388, 2498
BstMBI GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
BstMCI CGRYCG 1 cut(s) 390
BstMWI GCNNNNNNNGC 8 cut(s) 254, 674, 683, 1039, 1060, 1298, 1598, 2464
BstNSI RCATGY 5 cut(s) 398, 1185, 1305, 1690, 1790
BstSCI CCNGG 2 cut(s) 2609, 2610
BstSFI CTRYAG 3 cut(s) 553, 1197, 2597
BstSLI GKGCMC 1 cut(s) 54
BstV1I GCAGC 3 cut(s) 199, 269, 282
BstV2I GAAGAC 2 cut(s) 1733, 2280
BstX2I RGATCY 3 cut(s) 907, 2065, 2562
BstYI RGATCY 3 cut(s) 907, 2065, 2562
BstZ17I GTATAC 1 cut(s) 1567
Bsu15I ATCGAT 1 cut(s) 1889
BsuI GTATCC 2 cut(s) 436, 1186
BsuRI GGCC 6 cut(s) 110, 1601, 1665, 2255, 2458, 2609
BsuTUI ATCGAT 1 cut(s) 1889
BtgI CCRYGG 1 cut(s) 2566
BtrI CACGTC 2 cut(s) 1395, 1795
BtsIMutI CAGTG 6 cut(s) 90, 1284, 1303, 1335, 2105, 2232
BveI ACCTGC 1 cut(s) 2061
Cac8I GCNNGC 2 cut(s) 246, 2456
CfoI GCGC 1 cut(s) 69
Cfr10I RCCGGY 1 cut(s) 2454
Cfr13I GGNCC 4 cut(s) 155, 1003, 2008, 2608
Cfr9I CCCGGG 1 cut(s) 2610
ClaI ATCGAT 1 cut(s) 1889
Csp6I GTAC 4 cut(s) 507, 1319, 2534, 2670
CspCI CAANNNNNGTGG 2 cut(s) 2661, 2696
CviQI GTAC 4 cut(s) 507, 1319, 2534, 2670
DdeI CTNAG 5 cut(s) 137, 320, 1059, 2022, 2693
DpnI GATC 7 cut(s) 389, 909, 1038, 1674, 2067, 2239, 2564
DpnII GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 2 cut(s) 1599, 2456
Eam1104I CTCTTC 5 cut(s) 492, 636, 720, 1101, 1966
EarI CTCTTC 5 cut(s) 492, 636, 720, 1101, 1966
Ecl136II GAGCTC 2 cut(s) 182, 248
Eco130I CCWWGG 2 cut(s) 282, 2566
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 184, 250, 2179
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 3 cut(s) 155, 1003, 2008
Eco53kI GAGCTC 2 cut(s) 182, 248
Eco57I CTGAAG 4 cut(s) 1112, 1139, 1271, 2309
Eco88I CYCGRG 1 cut(s) 2610
EcoICRI GAGCTC 2 cut(s) 182, 248
EcoRI GAATTC 1 cut(s) 541
EcoRV GATATC 1 cut(s) 1481
EcoT14I CCWWGG 2 cut(s) 282, 2566
EcoT22I ATGCAT 2 cut(s) 438, 959
EcoT38I GRGCYC 3 cut(s) 184, 250, 2179
ErhI CCWWGG 2 cut(s) 282, 2566
FalI AAGNNNNNCTT 4 cut(s) 471, 503, 2164, 2196
FaqI GGGAC 1 cut(s) 1339
FauNDI CATATG 4 cut(s) 217, 752, 1188, 1207
FblI GTMKAC 1 cut(s) 1566
Fnu4HI GCNGC 3 cut(s) 213, 258, 271
FriOI GRGCYC 3 cut(s) 184, 250, 2179
Fsp4HI GCNGC 3 cut(s) 213, 258, 271
FspBI CTAG 8 cut(s) 423, 578, 647, 719, 1322, 1530, 1838, 2621
GlaI GCGC 1 cut(s) 68
GluI GCNGC 3 cut(s) 213, 258, 271
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 6 cut(s) 110, 1601, 1665, 2255, 2458, 2609
HapII CCGG 3 cut(s) 440, 2455, 2611
HhaI GCGC 1 cut(s) 69
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HincII GTYRAC 1 cut(s) 1792
HindII GTYRAC 1 cut(s) 1792
HindIII AAGCTT 2 cut(s) 1408, 2107
HpaII CCGG 3 cut(s) 440, 2455, 2611
HphI GGTGA 3 cut(s) 1250, 1812, 2273
Hpy166II GTNNAC 7 cut(s) 158, 622, 835, 1567, 1792, 2008, 2232
Hpy8I GTNNAC 7 cut(s) 158, 622, 835, 1567, 1792, 2008, 2232
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 8 cut(s) 94, 128, 197, 1007, 1142, 1330, 2661, 2706
HpyCH4IV ACGT 3 cut(s) 1394, 1794, 2268
HpyF10VI GCNNNNNNNGC 8 cut(s) 254, 674, 683, 1039, 1060, 1298, 1598, 2464
HpyF3I CTNAG 5 cut(s) 137, 320, 1059, 2022, 2693
HpySE526I ACGT 3 cut(s) 1394, 1794, 2268
HspAI GCGC 1 cut(s) 67
Kpn2I TCCGGA 1 cut(s) 439
KroI GCCGGC 1 cut(s) 2454
KroNI GCCGGC 1 cut(s) 2456
Kzo9I GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 6 cut(s) 50, 267, 949, 1901, 2035, 2182
Lsp1109I GCAGC 3 cut(s) 199, 269, 282
LweI GCATC 8 cut(s) 445, 731, 784, 944, 1072, 1646, 1768, 2017
MaeI CTAG 8 cut(s) 423, 578, 647, 719, 1322, 1530, 1838, 2621
MaeII ACGT 3 cut(s) 1394, 1794, 2268
MaeIII GTNAC 9 cut(s) 88, 632, 649, 679, 1177, 1382, 1678, 1818, 2071
MalI GATC 7 cut(s) 389, 909, 1038, 1674, 2067, 2239, 2564
MbiI CCGCTC 1 cut(s) 1348
MboI GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
MfeI CAATTG 2 cut(s) 161, 2148
MflI RGATCY 3 cut(s) 907, 2065, 2562
MhlI GDGCHC 6 cut(s) 54, 184, 250, 946, 1550, 2179
MlyI GAGTC 4 cut(s) 481, 1755, 1982, 2386
MmeI TCCRAC 1 cut(s) 1414
Mph1103I ATGCAT 2 cut(s) 438, 959
MroI TCCGGA 1 cut(s) 439
MroNI GCCGGC 1 cut(s) 2454
MroXI GAANNNNTTC 2 cut(s) 2481, 2577
MseI TTAA 5 cut(s) 240, 1610, 1944, 2111, 2226
MslI CAYNNNNRTG 2 cut(s) 1210, 1277
MspI CCGG 3 cut(s) 440, 2455, 2611
MspR9I CCNGG 2 cut(s) 2611, 2612
MunI CAATTG 2 cut(s) 161, 2148
Mva1269I GAATGC 1 cut(s) 356
MwoI GCNNNNNNNGC 8 cut(s) 254, 674, 683, 1039, 1060, 1298, 1598, 2464
NaeI GCCGGC 1 cut(s) 2456
NciI CCSGG 2 cut(s) 2611, 2612
NcoI CCATGG 1 cut(s) 2566
NdeI CATATG 4 cut(s) 217, 752, 1188, 1207
NdeII GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
NgoMIV GCCGGC 1 cut(s) 2454
NlaIV GGNNCC 5 cut(s) 76, 808, 2178, 2301, 2585
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 4 cut(s) 88, 649, 679, 1818
NsiI ATGCAT 2 cut(s) 438, 959
NspI RCATGY 5 cut(s) 398, 1185, 1305, 1690, 1790
OliI CACNNNNGTG 1 cut(s) 1277
PceI AGGCCT 1 cut(s) 110
PciI ACATGT 2 cut(s) 394, 1686
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 1 cut(s) 919
PctI GAATGC 1 cut(s) 356
PdiI GCCGGC 1 cut(s) 2456
PdmI GAANNNNTTC 2 cut(s) 2481, 2577
PfeI GAWTC 6 cut(s) 175, 757, 924, 1368, 1448, 1916
PkrI GCNGC 3 cut(s) 214, 259, 272
Ple19I CGATCG 1 cut(s) 390
PleI GAGTC 4 cut(s) 480, 1754, 1982, 2386
PpsI GAGTC 4 cut(s) 480, 1754, 1982, 2386
PscI ACATGT 2 cut(s) 394, 1686
Psp124BI GAGCTC 2 cut(s) 184, 250
PspN4I GGNNCC 5 cut(s) 76, 808, 2178, 2301, 2585
PspPI GGNCC 4 cut(s) 155, 1003, 2008, 2608
PstI CTGCAG 3 cut(s) 557, 1201, 2601
PsuI RGATCY 3 cut(s) 907, 2065, 2562
PvuI CGATCG 1 cut(s) 390
RsaI GTAC 4 cut(s) 508, 1320, 2535, 2671
RsaNI GTAC 4 cut(s) 507, 1319, 2534, 2670
RseI CAYNNNNRTG 2 cut(s) 1210, 1277
SacI GAGCTC 2 cut(s) 184, 250
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 5 cut(s) 240, 1610, 1944, 2111, 2226
SatI GCNGC 3 cut(s) 213, 258, 271
Sau3AI GATC 7 cut(s) 387, 907, 1036, 1672, 2065, 2237, 2562
Sau96I GGNCC 4 cut(s) 155, 1003, 2008, 2608
SchI GAGTC 4 cut(s) 481, 1755, 1982, 2386
ScrFI CCNGG 2 cut(s) 2611, 2612
SduI GDGCHC 6 cut(s) 54, 184, 250, 946, 1550, 2179
SfaNI GCATC 8 cut(s) 445, 731, 784, 944, 1072, 1646, 1768, 2017
SfcI CTRYAG 3 cut(s) 553, 1197, 2597
SinI GGWCC 3 cut(s) 155, 1003, 2008
SmaI CCCGGG 1 cut(s) 2612
SmiMI CAYNNNNRTG 2 cut(s) 1210, 1277
SmlI CTYRAG 2 cut(s) 1977, 2558
SmoI CTYRAG 2 cut(s) 1977, 2558
SseBI AGGCCT 1 cut(s) 110
SsiI CCGC 4 cut(s) 1348, 2015, 2156, 2334
SspI AATATT 3 cut(s) 9, 613, 2220
SspMI CTAG 8 cut(s) 423, 578, 647, 719, 1322, 1530, 1838, 2621
SstI GAGCTC 2 cut(s) 184, 250
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 2609, 2610
StyI CCWWGG 2 cut(s) 282, 2566
TaaI ACNGT 8 cut(s) 94, 128, 197, 1007, 1142, 1330, 2661, 2706
TaiI ACGT 3 cut(s) 1397, 1797, 2271
TaqI TCGA 9 cut(s) 12, 178, 913, 1375, 1744, 1854, 1889, 2390, 2475
TatI WGTACW 4 cut(s) 506, 1318, 2533, 2669
TfiI GAWTC 6 cut(s) 175, 757, 924, 1368, 1448, 1916
Tru1I TTAA 5 cut(s) 240, 1610, 1944, 2111, 2226
Tru9I TTAA 5 cut(s) 240, 1610, 1944, 2111, 2226
TscAI CASTG 6 cut(s) 97, 1284, 1303, 1335, 2105, 2239
TseFI GTSAC 4 cut(s) 88, 649, 679, 1818
TseI GCWGC 3 cut(s) 212, 257, 270
Tsp45I GTSAC 4 cut(s) 88, 649, 679, 1818
TspDTI ATGAA 8 cut(s) 555, 605, 654, 846, 1112, 2357, 2463, 2499
TspGWI ACGGA 3 cut(s) 37, 88, 1607
TspMI CCCGGG 1 cut(s) 2610
TspRI CASTG 6 cut(s) 97, 1284, 1303, 1335, 2105, 2239
VpaK11BI GGWCC 3 cut(s) 155, 1003, 2008
XapI RAATTY 6 cut(s) 117, 541, 1504, 1655, 2058, 2347
XbaI TCTAGA 1 cut(s) 1529
XceI RCATGY 5 cut(s) 398, 1185, 1305, 1690, 1790
XmaI CCCGGG 1 cut(s) 2610
XmiI GTMKAC 1 cut(s) 1566
XmnI GAANNNNTTC 2 cut(s) 2481, 2577
XspI CTAG 8 cut(s) 423, 578, 647, 719, 1322, 1530, 1838, 2621
Zsp2I ATGCAT 2 cut(s) 438, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.