Rh4BG074100

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001399
Physical Location & Seq
Reverse (-)
13067671 .. 13077252
9582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG074100.1

Sequence Viewer

Length: 2373 bp
ATGGGAATCTTCGAACCCTTCAGAGCAATCGGCTACACAACCACCTCCGTCCCCTTCTCCGTTCAGCGCCTCGGCACTGAAGCCTTCGTCACCGTCAGCGTCGGCAAAGCCTTCCAAATTTACAATTGCGCAAAGCTCACTCTGGTCCTCGTCGGTCCGCAATTGCCGAAGAAGATTCGAGCTCTTGCTTCGTATCGGGACTACACATTTGCTGCGTATGGGACTGATATTGCTGTTTTTAAGAGATCTCATCAGGTTGCTACGTGGAGCACCCATAATGCGAAGGTCAATTTGTTGCTATTGTTTGGAGAGCATATCCTAAGTGTTGATGTTGAAGGCAATCTGTATATATGGACATTTAAAGGAATTGATGAGAATCTTGATCCGGTTGGACATATTAAGTTGGACGAAAATTTTACTCCTAGCTGTATAATGCATCCGGATACCTACTTAAATAAGGTTATCATTGGGAGTCAAGAAGGTTCTCTGCAGCTTTGGAATATCAGCACAAAGAAAAAACTGTATGAGTTCAAGGGATGGAAATCTTCTATATGCAGCTGTGTTTCTTCTCCTGCGCTAGATGTTGTTGCAGTTGGTTGTGCTGATGGAAAAATTCATGTTCACAACATACGATTTGATGAAGAGTTAGTGACATTTTCCCATTCAACACGAGGTGCTGTGACTGCCTTATCTTTCAGCACAGATGGGCAGCCTCTTCTAGCATCTGGAGGTTCTTCTGGTGTTATAAGCATATGGAATCTCGAAAAAAGAAGGCTTCAATCAGTTATAAAAGAGGCACACGATGGTTCTATACTTTCACTGCACTTTTTTGTGAATGAACCAGTGTTAATGAGTTCATCATCAGACAATTCTATTAAAATGTGGATTTTTGACACAAGCGATGGAGATGCTCGTCTTTTACGGTTTAGAAGTGGTCATAGTGCTCCTCCACAATGCATAAGGTTTTATGCAAATGGGAGACATATTCTATCAGCCGGTCAGGATCGTGCCTTTCGCCTTTTCTCTATAATCCAGGATCAACAAAGTAGAGAGCTTTCTCAACGTCATATAACAAAACGAGCAAAGAAACTCAGGGTGAAGGAGGAAGAGATAAAACTGAAACCTGTCATTGCTTTTGATTGTGCTGAAATAAGAGAGCGAGATTGGTGCAATGTTGTTACATGTCATATGGATACTACACAGGCATATGTCTGGAGACTTCAAAACTTTGTTCTTGGGGAGCATATTCTGAAACCAAGACCAGAAAACCCAACACCTGTGAAGGCCTGTGCCATCAGTGCATGTGGAAATTTTGCCATACTAGGGACCGCTGGTGGTTGGATTGAGCGGTTCAACCTGCAGTCAGGAAGCAGCCGAGGTAATTATGTGGATCCATCGGAAAGAAGTATCTGTGCCCATGACGGGGAAGTTGTTGGAATTGCTTGTGATTCAACTAATACTCACATGATGAGTGCTGGATATCACGGAGACATAAAGGTCTGGGATTTCAAGGGACGTGGAGTGCCATCTAAATGGGAAGTTGGGAGCTCTGTTGTCAGGATTGTATTCAATCGCTTGAATGGTCTTTTGGCTGTTGCGGCTGATGACTTAATTATCCGCTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACTGATCGTGTTACAGACATGTGTTTTAGTGAGGATGGAAAATGGCTTTTGTCTTCCAGTATGGATGGAAGTCTTCGAGTTTGGGATGTTATCTTAGCAAGACAAATAGATGCGTTGCATGTTGATGTGTCTGTGACTGCATTGTCTCTATCACCGAATATGGATATTTTAGCGACCTCCCATGTTGATCAAAATGGGGTCTATCTTTGGGTGAATCAATCAATGTTTTCGGGAGCTTCAAAGGTAGATTCCTATGCAAGTGGAAAGCGAGTTGTGAGTGTTAAGTTGCCATCTGTCTCTTCGACAAAAGGTTCTGAAGCTGAGGATTCTGATGAGCCTGTTGGAAACTCTGAACAACTGAAAGATGCTTCTGCTCTTCCAAGTCCTGTACAGCAAATTCCAGATCTTGTTACTCTCTCATCATATCCTAAAAGCCAGTGGCAAAGCTTAATCAACTTAGACATTATAAAGGAACGAAATAAACCGATTGAGCCCCCAAAGAAACCTGAAAAGGCTCCTTTCTTTTTGCCTTCAGTTCCATCTCTTTCAGGAGAAATAATGTTTAAGCCTACTGGGTCGACAAATGAGGAGGCAAAAGGTGGTGAAGAATCATCTAGAATAAAGGCGGCTCTAGCGCCATCAATATTCCTTCAAGCGCTTCAGTCATCTGCAGAAATGAAGAATTGTAAATATGTTATCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

790

Amino Acids

87.3

Weight (kDa)

7.85

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 30 - 298 1.8e-100 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 103 - 260 2.3e-15 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 123 - 353 1.8e-16 G protein beta WD-40 repeat protein
WDR55 PF24796 134 - 255 1.1e-08 WDR55
Beta-prop_RIG_2nd PF23775 134 - 287 1.8e-07 RIG second beta-propeller
WD40_Prp19 PF24814 143 - 295 3e-17 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 146 - 299 2.5e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 259 3.6e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 152 - 258 1.3e-06 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 153 - 260 3.2e-12 WDR90/POC16, second beta-propeller
Beta-prop_WDR5 PF25175 154 - 295 2e-16 WDR5 beta-propeller domain
Beta-prop_HPS5 PF23756 154 - 286 1.6e-07 HPS5 beta-propeller
WDR90_beta-prop_4th PF23342 155 - 273 1.7e-07 WDR90, 4th beta-propeller
Beta-prop_WDR3_1st PF25173 155 - 343 2.1e-25 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 157 - 298 5.8e-15 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 175 - 343 2.3e-07 Aladin seven-bladed propeller
WDR55 PF24796 184 - 295 3.7e-10 WDR55
Beta-prop_VPS8 PF23410 194 - 290 5.4e-07 Vps8 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 223 - 360 1.6e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 225 - 346 9.2e-11 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 226 - 299 4.7e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 227 - 343 3e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 239 - 358 1.4e-08 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 239 - 343 3.5e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 319 - 636 3.9e-103 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 427 - 626 1.5e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 430 - 615 1.3e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 467 - 616 1.1e-14 Prp19 WD40 domain
WDR55 PF24796 472 - 586 2.5e-07 WDR55
Beta-prop_WDR3_1st PF25173 476 - 621 5.4e-20 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 480 - 585 5.4e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 482 - 618 1.1e-08 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 516 - 631 6.3e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 517 - 597 4.4e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 518 - 592 2.4e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 528 - 627 5.3e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR75_1st PF23869 533 - 609 7.8e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 549 - 585 3.8e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 550 - 621 3.9e-07 CAF1B/HIR1 beta-propeller domain
Utp21 PF04192 688 - 786 2e-26 Utp21 specific WD40 associated putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 746, 788, 2135
AasI GACNNNNNNGTC 2 cut(s) 1496, 1810
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 1365
AccBSI CCGCTC 1 cut(s) 1348
AccI GTMKAC 1 cut(s) 2246
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 6 cut(s) 158, 1329, 1348, 1598, 1618, 2294
AclWI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
AcsI RAATTY 6 cut(s) 117, 412, 612, 1309, 1655, 2064
AcuI CTGAAG 5 cut(s) 4, 99, 2004, 2184, 2312
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 1 cut(s) 2058
AfeI AGCGCT 1 cut(s) 2325
AfiI CCNNNNNNNGG 3 cut(s) 1323, 1422, 1423
AflIII ACRYGT 2 cut(s) 1181, 1686
AjiI CACGTC 1 cut(s) 1517
AjnI CCWGG 1 cut(s) 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 1571, 1603
Alw21I GWGCWC 4 cut(s) 184, 272, 946, 1550
Alw26I GTCTC 5 cut(s) 973, 1210, 1482, 1818, 1969
AlwI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
Aor13HI TCCGGA 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 2325
AoxI GGCC 2 cut(s) 1284, 1663
ApeKI GCWGC 5 cut(s) 212, 490, 555, 709, 1371
ApoI RAATTY 6 cut(s) 117, 412, 612, 1309, 1655, 2064
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 5 cut(s) 69, 131, 577, 2305, 2326
AspS9I GGNCC 3 cut(s) 145, 155, 1326
AsuHPI GGTGA 5 cut(s) 82, 1108, 1812, 1891, 2282
AsuII TTCGAA 1 cut(s) 12
AvaII GGWCC 3 cut(s) 145, 155, 1326
BaeGI GKGCMC 1 cut(s) 1417
BamHI GGATCC 1 cut(s) 1390
BanII GRGCYC 3 cut(s) 184, 1550, 2163
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 2 cut(s) 1713, 1733
Bbv12I GWGCWC 4 cut(s) 184, 272, 946, 1550
BbvCI CCTCAGC 1 cut(s) 1989
BbvI GCAGC 5 cut(s) 199, 502, 567, 721, 1383
BcgI CGANNNNNNTGC 4 cut(s) 890, 924, 1149, 1183
BciT130I CCWGG 1 cut(s) 1034
BciVI GTATCC 2 cut(s) 436, 1186
BclI TGATCA 1 cut(s) 1855
BcoDI GTCTC 5 cut(s) 973, 1210, 1482, 1818, 1969
BfaI CTAG 6 cut(s) 423, 578, 719, 1322, 2283, 2300
BfmI CTRYAG 3 cut(s) 488, 1358, 2337
BfoI RGCGCY 3 cut(s) 70, 2306, 2327
BfuAI ACCTGC 1 cut(s) 1365
BfuI GTATCC 2 cut(s) 436, 1186
BglII AGATCT 2 cut(s) 245, 2071
BisI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
BlsI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
Bme1390I CCNGG 1 cut(s) 1034
Bme18I GGWCC 3 cut(s) 145, 155, 1326
BmgBI CACGTC 1 cut(s) 1517
BmgT120I GGNCC 3 cut(s) 145, 155, 1326
BmiI GGNNCC 3 cut(s) 1327, 1392, 2184
BmrFI CCNGG 1 cut(s) 1034
BmrI ACTGGG 1 cut(s) 2250
BmsI GCATC 6 cut(s) 445, 731, 898, 1646, 1768, 2023
BmuI ACTGGG 1 cut(s) 2250
BpiI GAAGAC 2 cut(s) 1713, 1733
BpmI CTGGAG 2 cut(s) 747, 1234
Bpu10I CCTNAGC 1 cut(s) 1989
Bpu14I TTCGAA 1 cut(s) 12
BsaAI YACGTR 1 cut(s) 264
BsaBI GATNNNNATC 2 cut(s) 375, 541
BsaJI CCNNGG 2 cut(s) 70, 1375
BsaWI WCCGGW 2 cut(s) 385, 439
Bsc4I CCNNNNNNNGG 3 cut(s) 1323, 1422, 1423
Bse118I RCCGGY 1 cut(s) 995
Bse1I ACTGG 4 cut(s) 842, 1725, 2104, 2245
Bse3DI GCAATG 2 cut(s) 1128, 1177
Bse8I GATNNNNATC 2 cut(s) 375, 541
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 1 cut(s) 1034
BseDI CCNNGG 2 cut(s) 70, 1375
BseGI GGATG 5 cut(s) 436, 542, 1708, 1738, 1759
BseJI GATNNNNATC 2 cut(s) 375, 541
BseLI CCNNNNNNNGG 3 cut(s) 1323, 1422, 1423
BseMI GCAATG 2 cut(s) 1128, 1177
BseMII CTCAG 2 cut(s) 1105, 1980
BseNI ACTGG 4 cut(s) 842, 1725, 2104, 2245
BseRI GAGGAG 2 cut(s) 936, 2270
BseSI GKGCMC 1 cut(s) 1417
BseXI GCAGC 5 cut(s) 199, 502, 567, 721, 1383
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 2 cut(s) 1286, 1665
BsiHKAI GWGCWC 4 cut(s) 184, 272, 946, 1550
BsiSI CCGG 3 cut(s) 386, 440, 996
BslFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BslI CCNNNNNNNGG 3 cut(s) 1323, 1422, 1423
BsmAI GTCTC 5 cut(s) 973, 1210, 1482, 1818, 1969
BsmFI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
BsnI GGCC 2 cut(s) 1286, 1665
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 6 cut(s) 184, 272, 946, 1417, 1550, 2163
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 2056
Bsp143I GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
BspACI CCGC 6 cut(s) 158, 1329, 1348, 1598, 1618, 2294
BspANI GGCC 2 cut(s) 1286, 1665
BspCNI CTCAG 2 cut(s) 1104, 1981
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 1327, 1392, 2184
BspMAI CTGCAG 3 cut(s) 492, 1362, 2341
BspMI ACCTGC 1 cut(s) 1365
BspPI GGATC 5 cut(s) 377, 1011, 1044, 1385, 1398
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 1128, 1177
BsrFI RCCGGY 1 cut(s) 995
BsrGI TGTACA 1 cut(s) 2056
BsrI ACTGG 4 cut(s) 842, 1725, 2104, 2245
BssAI RCCGGY 1 cut(s) 995
BssECI CCNNGG 2 cut(s) 70, 1375
BssMI GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
BssSI CACGAG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 1 cut(s) 1034
Bst4CI ACNGT 3 cut(s) 94, 522, 924
Bst6I CTCTTC 5 cut(s) 636, 720, 1101, 1972, 2049
BstAUI TGTACA 1 cut(s) 2056
BstBAI YACGTR 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 12
BstDEI CTNAG 5 cut(s) 320, 1091, 1762, 1989, 2125
BstF5I GGATG 5 cut(s) 436, 542, 1708, 1738, 1759
BstH2I RGCGCY 3 cut(s) 70, 2306, 2327
BstHHI GCGC 5 cut(s) 69, 131, 577, 2305, 2326
BstKTI GATC 8 cut(s) 248, 385, 1006, 1039, 1393, 1675, 1858, 2074
BstMAI GTCTC 5 cut(s) 973, 1210, 1482, 1818, 1969
BstMBI GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
BstMWI GCNNNNNNNGC 4 cut(s) 683, 1298, 1598, 2300
BstNI CCWGG 1 cut(s) 1034
BstNSI RCATGY 4 cut(s) 1185, 1305, 1690, 1790
BstSCI CCNGG 1 cut(s) 1032
BstSFI CTRYAG 3 cut(s) 488, 1358, 2337
BstSLI GKGCMC 1 cut(s) 1417
BstV1I GCAGC 5 cut(s) 199, 502, 567, 721, 1383
BstV2I GAAGAC 2 cut(s) 1713, 1733
BstX2I RGATCY 3 cut(s) 245, 1390, 2071
BstXI CCANNNNNNTGG 1 cut(s) 1533
BstYI RGATCY 3 cut(s) 245, 1390, 2071
BsuI GTATCC 2 cut(s) 436, 1186
BsuRI GGCC 2 cut(s) 1286, 1665
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 1517
BtsCI GGATG 5 cut(s) 436, 542, 1708, 1738, 1759
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 5 cut(s) 75, 818, 849, 1303, 2111
BveI ACCTGC 1 cut(s) 1365
CfoI GCGC 5 cut(s) 69, 131, 577, 2305, 2326
Cfr10I RCCGGY 1 cut(s) 995
Cfr13I GGNCC 3 cut(s) 145, 155, 1326
CpoI CGGWCCG 1 cut(s) 155
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 2057
CspCI CAANNNNNGTGG 2 cut(s) 1498, 1533
CspI CGGWCCG 1 cut(s) 155
CviAII CATG 8 cut(s) 617, 1182, 1302, 1418, 1465, 1687, 1787, 1850
CviQI GTAC 1 cut(s) 2057
DdeI CTNAG 5 cut(s) 320, 1091, 1762, 1989, 2125
DpnI GATC 8 cut(s) 247, 384, 1005, 1038, 1392, 1674, 1857, 2073
DpnII GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 674
DrdI GACNNNNNNGTC 2 cut(s) 1496, 1810
DseDI GACNNNNNNGTC 2 cut(s) 1496, 1810
Eam1104I CTCTTC 5 cut(s) 636, 720, 1101, 1972, 2049
EarI CTCTTC 5 cut(s) 636, 720, 1101, 1972, 2049
Ecl136II GAGCTC 2 cut(s) 182, 1548
Eco147I AGGCCT 1 cut(s) 1286
Eco24I GRGCYC 3 cut(s) 184, 1550, 2163
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 3 cut(s) 145, 155, 1326
Eco47III AGCGCT 1 cut(s) 2325
Eco53kI GAGCTC 2 cut(s) 182, 1548
Eco57I CTGAAG 5 cut(s) 4, 99, 2004, 2184, 2312
EcoICRI GAGCTC 2 cut(s) 182, 1548
EcoRII CCWGG 1 cut(s) 1032
EcoRV GATATC 1 cut(s) 1481
EcoT22I ATGCAT 2 cut(s) 438, 959
EcoT38I GRGCYC 3 cut(s) 184, 1550, 2163
FaeI CATG 8 cut(s) 620, 1185, 1305, 1421, 1468, 1690, 1790, 1853
FaqI GGGAC 5 cut(s) 35, 212, 235, 1339, 1527
FatI CATG 8 cut(s) 616, 1181, 1301, 1417, 1464, 1686, 1786, 1849
FauNDI CATATG 3 cut(s) 752, 1188, 1207
FbaI TGATCA 1 cut(s) 1855
FblI GTMKAC 1 cut(s) 2246
Fnu4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FokI GGATG 5 cut(s) 423, 549, 1715, 1745, 1766
FriOI GRGCYC 3 cut(s) 184, 1550, 2163
Fsp4HI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
FspBI CTAG 6 cut(s) 423, 578, 719, 1322, 2283, 2300
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 5 cut(s) 68, 130, 576, 2304, 2325
GluI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
GsuI CTGGAG 2 cut(s) 747, 1234
HaeII RGCGCY 3 cut(s) 70, 2306, 2327
HaeIII GGCC 2 cut(s) 1286, 1665
HapII CCGG 3 cut(s) 386, 440, 996
HgaI GACGC 1 cut(s) 88
HhaI GCGC 5 cut(s) 69, 131, 577, 2305, 2326
Hin1II CATG 8 cut(s) 620, 1185, 1305, 1421, 1468, 1690, 1790, 1853
Hin6I GCGC 5 cut(s) 67, 129, 575, 2303, 2324
HinP1I GCGC 5 cut(s) 67, 129, 575, 2303, 2324
HincII GTYRAC 1 cut(s) 2247
HindII GTYRAC 1 cut(s) 2247
HindIII AAGCTT 1 cut(s) 2113
HpaII CCGG 3 cut(s) 386, 440, 996
HphI GGTGA 5 cut(s) 82, 1108, 1812, 1891, 2282
Hpy166II GTNNAC 2 cut(s) 622, 2247
Hpy188I TCNGA 7 cut(s) 23, 865, 1251, 1399, 1984, 1999, 2020
Hpy8I GTNNAC 2 cut(s) 622, 2247
Hpy99I CGWCG 2 cut(s) 104, 155
HpyCH4III ACNGT 3 cut(s) 94, 522, 924
HpyCH4IV ACGT 3 cut(s) 263, 1063, 1516
HpyF10VI GCNNNNNNNGC 4 cut(s) 683, 1298, 1598, 2300
HpyF3I CTNAG 5 cut(s) 320, 1091, 1762, 1989, 2125
HpySE526I ACGT 3 cut(s) 263, 1063, 1516
Hsp92II CATG 8 cut(s) 620, 1185, 1305, 1421, 1468, 1690, 1790, 1853
HspAI GCGC 5 cut(s) 67, 129, 575, 2303, 2324
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 1 cut(s) 1855
Kzo9I GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
LguI GCTCTTC 1 cut(s) 2049
LmnI GCTCC 6 cut(s) 267, 949, 1240, 1545, 1901, 2188
Lsp1109I GCAGC 5 cut(s) 199, 502, 567, 721, 1383
LweI GCATC 6 cut(s) 445, 731, 898, 1646, 1768, 2023
MaeI CTAG 6 cut(s) 423, 578, 719, 1322, 2283, 2300
MaeII ACGT 3 cut(s) 263, 1063, 1516
MaeIII GTNAC 7 cut(s) 88, 649, 679, 1177, 1678, 1801, 2077
MalI GATC 8 cut(s) 247, 384, 1005, 1038, 1392, 1674, 1857, 2073
MbiI CCGCTC 1 cut(s) 1348
MboI GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
MfeI CAATTG 2 cut(s) 124, 161
MflI RGATCY 3 cut(s) 245, 1390, 2071
MhlI GDGCHC 6 cut(s) 184, 272, 946, 1417, 1550, 2163
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 5 cut(s) 370, 384, 1319, 1414, 1990
Mph1103I ATGCAT 2 cut(s) 438, 959
MroI TCCGGA 1 cut(s) 439
MslI CAYNNNNRTG 2 cut(s) 1531, 1791
MspA1I CMGCKG 2 cut(s) 558, 1331
MspI CCGG 3 cut(s) 386, 440, 996
MspR9I CCNGG 1 cut(s) 1034
MunI CAATTG 2 cut(s) 124, 161
MvaI CCWGG 1 cut(s) 1034
MwoI GCNNNNNNNGC 4 cut(s) 683, 1298, 1598, 2300
NdeI CATATG 3 cut(s) 752, 1188, 1207
NdeII GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
NlaIII CATG 8 cut(s) 620, 1185, 1305, 1421, 1468, 1690, 1790, 1853
NlaIV GGNNCC 3 cut(s) 1327, 1392, 2184
NmeAIII GCCGAG 2 cut(s) 51, 1400
NmuCI GTSAC 4 cut(s) 88, 649, 679, 1801
NsbI TGCGCA 1 cut(s) 130
NsiI ATGCAT 2 cut(s) 438, 959
NspI RCATGY 4 cut(s) 1185, 1305, 1690, 1790
NspV TTCGAA 1 cut(s) 12
PceI AGGCCT 1 cut(s) 1286
PciI ACATGT 2 cut(s) 1181, 1686
PciSI GCTCTTC 1 cut(s) 2049
PfeI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 7 cut(s) 214, 492, 557, 711, 1373, 1600, 2296
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 1 cut(s) 264
PscI ACATGT 2 cut(s) 1181, 1686
PsiI TTATAA 3 cut(s) 746, 788, 2135
Psp124BI GAGCTC 2 cut(s) 184, 1550
Psp6I CCWGG 1 cut(s) 1032
PspGI CCWGG 1 cut(s) 1032
PspN4I GGNNCC 3 cut(s) 1327, 1392, 2184
PspPI GGNCC 3 cut(s) 145, 155, 1326
PstI CTGCAG 3 cut(s) 492, 1362, 2341
PsuI RGATCY 3 cut(s) 245, 1390, 2071
PvuII CAGCTG 1 cut(s) 558
RsaI GTAC 1 cut(s) 2058
RsaNI GTAC 1 cut(s) 2057
RseI CAYNNNNRTG 2 cut(s) 1531, 1791
Rsr2I CGGWCCG 1 cut(s) 155
RsrII CGGWCCG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 184, 1550
SalI GTCGAC 1 cut(s) 2245
SapI GCTCTTC 1 cut(s) 2049
SatI GCNGC 7 cut(s) 213, 491, 556, 710, 1372, 1599, 2295
Sau3AI GATC 8 cut(s) 245, 382, 1003, 1036, 1390, 1672, 1855, 2071
Sau96I GGNCC 3 cut(s) 145, 155, 1326
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 1 cut(s) 1034
SduI GDGCHC 6 cut(s) 184, 272, 946, 1417, 1550, 2163
SfaNI GCATC 6 cut(s) 445, 731, 898, 1646, 1768, 2023
SfcI CTRYAG 3 cut(s) 488, 1358, 2337
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 3 cut(s) 145, 155, 1326
SmiMI CAYNNNNRTG 2 cut(s) 1531, 1791
SseBI AGGCCT 1 cut(s) 1286
SsiI CCGC 6 cut(s) 158, 1329, 1348, 1598, 1618, 2294
SspI AATATT 1 cut(s) 2313
SspMI CTAG 6 cut(s) 423, 578, 719, 1322, 2283, 2300
SstI GAGCTC 2 cut(s) 184, 1550
StuI AGGCCT 1 cut(s) 1286
StyD4I CCNGG 1 cut(s) 1032
TaaI ACNGT 3 cut(s) 94, 522, 924
TaiI ACGT 3 cut(s) 266, 1066, 1519
TaqI TCGA 6 cut(s) 12, 178, 762, 1744, 1970, 2246
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 1 cut(s) 2056
TauI GCSGC 2 cut(s) 1601, 2297
TfiI GAWTC 9 cut(s) 6, 175, 376, 757, 1448, 1882, 1916, 1994, 2276
TscAI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
TseFI GTSAC 4 cut(s) 88, 649, 679, 1801
TseI GCWGC 5 cut(s) 212, 490, 555, 709, 1371
Tsp45I GTSAC 4 cut(s) 88, 649, 679, 1801
TspDTI ATGAA 5 cut(s) 605, 654, 846, 852, 2360
TspGWI ACGGA 3 cut(s) 37, 49, 1500
TspRI CASTG 5 cut(s) 82, 825, 849, 1303, 2111
VpaK11BI GGWCC 3 cut(s) 145, 155, 1326
XapI RAATTY 6 cut(s) 117, 412, 612, 1309, 1655, 2064
XbaI TCTAGA 1 cut(s) 2282
XceI RCATGY 4 cut(s) 1185, 1305, 1690, 1790
XmiI GTMKAC 1 cut(s) 2246
XspI CTAG 6 cut(s) 423, 578, 719, 1322, 2283, 2300
Zsp2I ATGCAT 2 cut(s) 438, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.