pycom13g20470

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001399
Physical Location & Seq
Reverse (-)
16592114 .. 16594731
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20470.4

Sequence Viewer

Length: 1206 bp
ATGGACACGTCAGAAAGAAGAAGCTTTGCTCATGATGGGGAAGTGGTTGGAATTGCTTGTGATTCAACAAATACTCTAATGATAAGCGCAGGATATCATGGAGACATAAAGGTTTGGAATTTCAAGGGGCGTGATGTAAAATCTAGATGGGAAGTTGGGTGCTCTGTTGTCAAGATTGTATACAATCGTTTGAATGGTCTTTTGGCTGTTGTGGCTGATGACTTAATCATCCGTTTGTTTGATGTTGTGGCATCAAGAATGGTTCGTAAATTTGAAGGCCATACAGATTGTGTTACAGACATGTGTTTTAGCGAGGATGGAAAATGGTTTTTGTCATCAAGTATGGATGGAAATCTTCGAGTCTGGGATGTTATTTTAGCAAGACAAATAGATGCATTGCATGTTGACGTGTCTATAACAGCATTGTCTCTATCACCGAATATGGATGTTCTAGCGACTGCTCATGTTGACCAAAATGGGGTCTATCTTTGGGTAAATCAATCGATGTTTTCTGGAGCTTCAAAAGTTGATTCTTATGCAAGTGGAAAGGAAGTTAGGAGTGTTAAGTTGCCATCTATCTCTTCAACAAAAGGTTATCAAGAAGAGGACTCTGACGAACCTATTGTGGACCATCCGCAGTCTAAATATGCTCCTGCTTGTAAAACTCTTGACCTGCAAATTCCAGATCTTGTTACTCTCTCTTTATTGCCTAAAAGCCAGTGGCAAAGCTTAATCAATTTAGGCACTATAAAGGAACGAAATAAACCAATTGAACTGCCAAAAAAACCCGAAAGGGCTCCTTTCTTTTTGCCTTCAATTCCCTCCTTTTCAGGAGAAATATTATTTAAGCCCACTGGATCAGAAAATGAGGAGGCCAAAGGTGATGACATAGAAGACACTAGAATAAAATCTGGTCTGGCACCATCACAATTCTTGCAACATCTTCAGTCGTCAGCAGAAATGAAGAATTTTTCAGCATTTACTGACTATATCAAAAGTTTGTCTCCATCGACTCTGGATATGGAACTCCAAATGCTTCAGGTTGTTGATGATGAGGAGGAAGAACTTGAAAGCCGGCCAGAGTTGCTTTCAATCGAACTGCTTCTTGATTATTCTATACATGAGACTTCATGTAGAAACAATTTTGACTTTGTACAAGCTGTAATCAAGGTGTTTCTCAAGATCCATGGCGAAACAATTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
ANAPC4_WD40 Utp21 WD40

Protein Analysis

402

Amino Acids

44.89

Weight (kDa)

4.93

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 681
AccB1I GGYRCC 1 cut(s) 919
AccI GTMKAC 1 cut(s) 180
AciI CCGC 1 cut(s) 635
AclWI GGATC 2 cut(s) 865, 1177
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 4 cut(s) 118, 269, 678, 967
AcuI CTGAAG 2 cut(s) 929, 1022
AfaI GTAC 1 cut(s) 1155
AfiI CCNNNNNNNGG 1 cut(s) 478
AflIII ACRYGT 3 cut(s) 6, 300, 408
AjiI CACGTC 2 cut(s) 9, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 24, 518, 729, 1160
AluI AGCT 4 cut(s) 24, 518, 729, 1160
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 4 cut(s) 96, 432, 1008, 1118
AlwI GGATC 2 cut(s) 865, 1177
AoxI GGCC 3 cut(s) 277, 873, 1076
ApoI RAATTY 4 cut(s) 118, 269, 678, 967
Asp700I GAANNNNTTC 2 cut(s) 1101, 1197
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 1 cut(s) 628
AsuHPI GGTGA 2 cut(s) 426, 893
AvaII GGWCC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 919
BanII GRGCYC 1 cut(s) 799
BbsI GAAGAC 1 cut(s) 900
Bbv12I GWGCWC 1 cut(s) 164
BccI CCATC 8 cut(s) 29, 141, 311, 341, 580, 639, 931, 1015
BcoDI GTCTC 4 cut(s) 96, 432, 1008, 1118
BfaI CTAG 3 cut(s) 144, 452, 900
BfuAI ACCTGC 1 cut(s) 681
BglII AGATCT 1 cut(s) 685
Bme18I GGWCC 1 cut(s) 628
BmgBI CACGTC 2 cut(s) 9, 409
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 2 cut(s) 798, 921
BmsI GCATC 2 cut(s) 260, 382
BpiI GAAGAC 1 cut(s) 900
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 1163
Bsa29I ATCGAT 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 1186
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 2 cut(s) 718, 859
Bse3DI GCAATG 1 cut(s) 395
Bse8I GATNNNNATC 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 1186
BseGI GGATG 6 cut(s) 228, 322, 352, 373, 451, 631
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMI GCAATG 1 cut(s) 395
BseNI ACTGG 2 cut(s) 718, 859
BseRI GAGGAG 2 cut(s) 884, 1070
BshFI GGCC 3 cut(s) 279, 875, 1078
BshNI GGYRCC 1 cut(s) 919
BshVI ATCGAT 1 cut(s) 503
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 1075
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 4 cut(s) 96, 432, 1008, 1118
BsnI GGCC 3 cut(s) 279, 875, 1078
Bsp1286I GDGCHC 2 cut(s) 164, 799
Bsp1407I TGTACA 1 cut(s) 1153
Bsp143I GATC 3 cut(s) 685, 857, 1182
Bsp19I CCATGG 1 cut(s) 1186
BspACI CCGC 1 cut(s) 635
BspANI GGCC 3 cut(s) 279, 875, 1078
BspDI ATCGAT 1 cut(s) 503
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 798, 921
BspMI ACCTGC 1 cut(s) 681
BspPI GGATC 2 cut(s) 865, 1177
BspT107I GGYRCC 1 cut(s) 919
BsrDI GCAATG 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 1074
BsrGI TGTACA 1 cut(s) 1153
BsrI ACTGG 2 cut(s) 718, 859
BssAI RCCGGY 1 cut(s) 1074
BssECI CCNNGG 1 cut(s) 1186
BssMI GATC 3 cut(s) 685, 857, 1182
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 1186
Bst1107I GTATAC 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 586, 597
BstAUI TGTACA 1 cut(s) 1153
BstC8I GCNNGC 1 cut(s) 1076
BstDSI CCRYGG 1 cut(s) 1186
BstF5I GGATG 6 cut(s) 228, 322, 352, 373, 451, 631
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 3 cut(s) 688, 860, 1185
BstMAI GTCTC 4 cut(s) 96, 432, 1008, 1118
BstMBI GATC 3 cut(s) 685, 857, 1182
BstMWI GCNNNNNNNGC 2 cut(s) 212, 1084
BstNSI RCATGY 2 cut(s) 304, 404
BstV2I GAAGAC 1 cut(s) 900
BstX2I RGATCY 2 cut(s) 685, 1182
BstYI RGATCY 2 cut(s) 685, 1182
BstZ17I GTATAC 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 503
BsuRI GGCC 3 cut(s) 279, 875, 1078
BsuTUI ATCGAT 1 cut(s) 503
BtgI CCRYGG 1 cut(s) 1186
BtrI CACGTC 2 cut(s) 9, 409
BtsCI GGATG 6 cut(s) 228, 322, 352, 373, 451, 631
BtsIMutI CAGTG 2 cut(s) 725, 852
BveI ACCTGC 1 cut(s) 681
Cac8I GCNNGC 1 cut(s) 1076
CciI TCATGA 1 cut(s) 31
CfoI GCGC 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 1 cut(s) 628
ClaI ATCGAT 1 cut(s) 503
Csp6I GTAC 1 cut(s) 1154
CviAII CATG 8 cut(s) 32, 98, 301, 401, 464, 1121, 1131, 1187
CviQI GTAC 1 cut(s) 1154
DpnI GATC 3 cut(s) 687, 859, 1184
DpnII GATC 3 cut(s) 685, 857, 1182
EaeI YGGCCR 1 cut(s) 1076
Eam1104I CTCTTC 2 cut(s) 586, 597
EarI CTCTTC 2 cut(s) 586, 597
Eco130I CCWWGG 1 cut(s) 1186
Eco24I GRGCYC 1 cut(s) 799
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 628
Eco57I CTGAAG 2 cut(s) 929, 1022
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 1186
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 1186
FaeI CATG 8 cut(s) 35, 101, 304, 404, 467, 1124, 1134, 1190
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FatI CATG 8 cut(s) 31, 97, 300, 400, 463, 1120, 1130, 1186
FblI GTMKAC 1 cut(s) 180
FokI GGATG 6 cut(s) 215, 329, 359, 380, 458, 618
FriOI GRGCYC 1 cut(s) 799
FspBI CTAG 3 cut(s) 144, 452, 900
GlaI GCGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 279, 875, 1078
HapII CCGG 1 cut(s) 1075
HhaI GCGC 1 cut(s) 89
Hin1II CATG 8 cut(s) 35, 101, 304, 404, 467, 1124, 1134, 1190
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 2 cut(s) 406, 469
HindII GTYRAC 2 cut(s) 406, 469
HindIII AAGCTT 2 cut(s) 22, 727
HinfI GANTC 5 cut(s) 62, 360, 530, 608, 1012
HpaII CCGG 1 cut(s) 1075
HphI GGTGA 2 cut(s) 426, 893
Hpy166II GTNNAC 4 cut(s) 181, 406, 469, 628
Hpy188I TCNGA 3 cut(s) 13, 613, 862
Hpy8I GTNNAC 4 cut(s) 181, 406, 469, 628
HpyAV CCTTC 2 cut(s) 269, 822
HpyCH4IV ACGT 2 cut(s) 8, 408
HpyCH4V TGCA 5 cut(s) 395, 400, 539, 676, 937
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 1084
HpySE526I ACGT 2 cut(s) 8, 408
Hsp92II CATG 8 cut(s) 35, 101, 304, 404, 467, 1124, 1134, 1190
HspAI GCGC 1 cut(s) 87
KroI GCCGGC 1 cut(s) 1074
KroNI GCCGGC 1 cut(s) 1076
Kzo9I GATC 3 cut(s) 685, 857, 1182
LmnI GCTCC 3 cut(s) 515, 655, 802
LweI GCATC 2 cut(s) 260, 382
MaeI CTAG 3 cut(s) 144, 452, 900
MaeII ACGT 2 cut(s) 8, 408
MaeIII GTNAC 2 cut(s) 292, 691
MalI GATC 3 cut(s) 687, 859, 1184
MboI GATC 3 cut(s) 685, 857, 1182
MboII GAAGA 8 cut(s) 30, 347, 573, 614, 905, 935, 976, 1073
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 685, 1182
MhlI GDGCHC 2 cut(s) 164, 799
MlyI GAGTC 3 cut(s) 369, 602, 1006
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 7 cut(s) 307, 598, 832, 862, 865, 1048, 1051
Mph1103I ATGCAT 1 cut(s) 397
MroNI GCCGGC 1 cut(s) 1074
MroXI GAANNNNTTC 2 cut(s) 1101, 1197
MseI TTAA 4 cut(s) 224, 564, 731, 846
MspI CCGG 1 cut(s) 1075
MunI CAATTG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 212, 1084
NaeI GCCGGC 1 cut(s) 1076
NcoI CCATGG 1 cut(s) 1186
NdeII GATC 3 cut(s) 685, 857, 1182
NgoMIV GCCGGC 1 cut(s) 1074
NlaIII CATG 8 cut(s) 35, 101, 304, 404, 467, 1124, 1134, 1190
NlaIV GGNNCC 2 cut(s) 798, 921
NsiI ATGCAT 1 cut(s) 397
NspI RCATGY 2 cut(s) 304, 404
PagI TCATGA 1 cut(s) 31
PciI ACATGT 1 cut(s) 300
PdiI GCCGGC 1 cut(s) 1076
PdmI GAANNNNTTC 2 cut(s) 1101, 1197
PfeI GAWTC 2 cut(s) 62, 530
PleI GAGTC 3 cut(s) 368, 602, 1006
PpsI GAGTC 3 cut(s) 368, 602, 1006
PscI ACATGT 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 798, 921
PspPI GGNCC 1 cut(s) 628
PsuI RGATCY 2 cut(s) 685, 1182
RsaI GTAC 1 cut(s) 1155
RsaNI GTAC 1 cut(s) 1154
SaqAI TTAA 4 cut(s) 224, 564, 731, 846
Sau3AI GATC 3 cut(s) 685, 857, 1182
Sau96I GGNCC 1 cut(s) 628
SchI GAGTC 3 cut(s) 369, 602, 1006
SduI GDGCHC 2 cut(s) 164, 799
SfaNI GCATC 2 cut(s) 260, 382
SinI GGWCC 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
SsiI CCGC 1 cut(s) 635
SspI AATATT 1 cut(s) 840
SspMI CTAG 3 cut(s) 144, 452, 900
StyI CCWWGG 1 cut(s) 1186
TaiI ACGT 2 cut(s) 11, 411
TaqI TCGA 4 cut(s) 358, 503, 1010, 1095
TatI WGTACW 1 cut(s) 1153
TfiI GAWTC 2 cut(s) 62, 530
Tru1I TTAA 4 cut(s) 224, 564, 731, 846
Tru9I TTAA 4 cut(s) 224, 564, 731, 846
TscAI CASTG 2 cut(s) 725, 859
TspDTI ATGAA 2 cut(s) 977, 1119
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 725, 859
VpaK11BI GGWCC 1 cut(s) 628
XapI RAATTY 4 cut(s) 118, 269, 678, 967
XbaI TCTAGA 1 cut(s) 143
XceI RCATGY 2 cut(s) 304, 404
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 1101, 1197
XspI CTAG 3 cut(s) 144, 452, 900
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.